SitesBLAST
Comparing 200846 FitnessBrowser__MR1:200846 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3bptA Crystal structure of human beta-hydroxyisobutyryl-coa hydrolase in complex with quercetin
35% identity, 86% coverage: 30:360/383 of query aligns to 15:336/362 of 3bptA
- active site: G67 (= G82), P84 (≠ K103), R88 (≠ E107), G115 (= G134), G118 (= G137), E138 (= E157), D146 (= D165)
- binding (2r)-3-hydroxy-2-methylpropanoic acid: G66 (= G81), G67 (= G82), I69 (≠ V84), E90 (= E109), G114 (= G133), G115 (= G134), E138 (= E157), D146 (= D165), V147 (= V166)
- binding 3,5,7,3',4'-pentahydroxyflavone: F25 (≠ A40), L26 (= L41), A28 (= A43), G66 (= G81), G67 (= G82), I69 (≠ V84), P137 (= P156), I141 (= I160), L319 (= L343)
Q9LKJ1 3-hydroxyisobutyryl-CoA hydrolase 1; CoA-thioester hydrolase CHY1; EC 3.1.2.-; EC 3.1.2.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
33% identity, 88% coverage: 30:366/383 of query aligns to 19:350/378 of Q9LKJ1
- G70 (= G82) mutation to S: Loss of activity.
- E142 (= E157) mutation to A: Loss of activity.
- D150 (= D165) mutation to G: Reduced activity.
4hdtA Crystal structure of a carnitinyl-coa dehydratase from mycobacterium thermoresistibile (see paper)
37% identity, 88% coverage: 30:366/383 of query aligns to 12:325/340 of 4hdtA
- active site: G64 (= G82), I69 (≠ L87), W84 (≠ F106), Y88 (= Y110), G112 (= G134), G115 (= G137), E135 (= E157), P142 (= P164), D143 (= D165), R283 (≠ K320)
- binding zinc ion: H28 (≠ L46), E42 (≠ K60), E57 (= E75), E79 (≠ Q97), H93 (≠ L115), H185 (≠ R207)
Sites not aligning to the query:
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
30% identity, 53% coverage: 17:219/383 of query aligns to 1:192/255 of 3q0jC
- active site: A65 (≠ G82), M70 (≠ V101), T80 (≠ R111), F84 (≠ L115), G108 (= G134), E111 (≠ G137), P130 (= P156), E131 (= E157), V136 (≠ L162), P138 (= P164), G139 (≠ D165)
- binding acetoacetyl-coenzyme a: Q23 (≠ K39), A24 (= A40), L25 (= L41), A27 (= A43), A63 (= A80), G64 (= G81), A65 (≠ G82), D66 (= D83), I67 (≠ V84), K68 (≠ R85), M70 (≠ V101), F84 (≠ L115), G107 (= G133), G108 (= G134), E111 (≠ G137), P130 (= P156), E131 (= E157), P138 (= P164), G139 (≠ D165), M140 (≠ V166)
Sites not aligning to the query:
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
30% identity, 53% coverage: 17:219/383 of query aligns to 1:192/255 of 3q0gC
- active site: A65 (≠ G82), M70 (≠ V101), T80 (≠ R111), F84 (≠ L115), G108 (= G134), E111 (≠ G137), P130 (= P156), E131 (= E157), V136 (≠ L162), P138 (= P164), G139 (≠ D165)
- binding coenzyme a: L25 (= L41), A63 (= A80), I67 (≠ V84), K68 (≠ R85), Y104 (≠ I130), P130 (= P156), E131 (= E157), L134 (≠ I160)
Sites not aligning to the query:
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
30% identity, 53% coverage: 18:219/383 of query aligns to 1:191/256 of 3h81A
- active site: A64 (≠ G82), M69 (≠ V101), T79 (≠ R111), F83 (≠ L115), G107 (= G134), E110 (≠ G137), P129 (= P156), E130 (= E157), V135 (≠ L162), P137 (= P164), G138 (≠ D165)
Sites not aligning to the query:
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
32% identity, 47% coverage: 22:200/383 of query aligns to 7:178/260 of 2hw5C
- active site: A68 (≠ G82), M73 (≠ L87), S83 (≠ R111), L87 (= L115), G111 (= G134), E114 (≠ G137), P133 (= P156), E134 (= E157), T139 (≠ L162), P141 (= P164), G142 (≠ D165)
- binding crotonyl coenzyme a: K26 (= K39), A27 (= A40), L28 (= L41), A30 (= A43), K62 (= K76), I70 (≠ V84), F109 (≠ M132)
Sites not aligning to the query:
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
30% identity, 53% coverage: 18:219/383 of query aligns to 1:187/250 of 3q0gD
- active site: A64 (≠ G82), M69 (≠ F105), T75 (≠ R111), F79 (≠ L115), G103 (= G134), E106 (≠ G137), P125 (= P156), E126 (= E157), V131 (≠ L162), P133 (= P164), G134 (≠ D165)
Sites not aligning to the query:
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
26% identity, 48% coverage: 19:203/383 of query aligns to 3:180/259 of 5zaiC
- active site: A65 (≠ G82), F70 (≠ L87), S82 (≠ T99), R86 (≠ K103), G110 (= G134), E113 (≠ G137), P132 (= P156), E133 (= E157), I138 (≠ L162), P140 (= P164), G141 (≠ D165)
- binding coenzyme a: K24 (≠ A40), L25 (= L41), A63 (= A80), G64 (= G81), A65 (≠ G82), D66 (= D83), I67 (≠ V84), P132 (= P156), R166 (≠ H189)
Sites not aligning to the query:
6z1pBI mS93 (see paper)
29% identity, 50% coverage: 9:201/383 of query aligns to 12:201/1413 of 6z1pBI
- active site: T85 (≠ G82), S134 (≠ G134), E157 (= E157), D165 (= D165)
- binding : S12 (≠ A9), S14 (= S11), Y41 (≠ K39), K42 (≠ A40), Q43 (≠ L41), T45 (≠ A43), D47 (= D45), H49 (≠ D47), K83 (≠ A80), T85 (≠ G82), D86 (= D83), F87 (≠ V84), K88 (≠ R85), K92 (≠ H89), L130 (≠ I130), K152 (≠ R152)
Sites not aligning to the query:
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
31% identity, 45% coverage: 30:200/383 of query aligns to 47:208/290 of P14604
- E144 (≠ G137) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E157) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
31% identity, 45% coverage: 30:200/383 of query aligns to 17:178/260 of 1dubA
- active site: A68 (≠ G82), M73 (≠ L87), S83 (≠ R111), L87 (= L115), G111 (= G134), E114 (≠ G137), P133 (= P156), E134 (= E157), T139 (≠ L162), P141 (= P164), G142 (≠ D165)
- binding acetoacetyl-coenzyme a: K26 (= K39), A27 (= A40), L28 (= L41), A30 (= A43), A66 (= A80), A68 (≠ G82), D69 (= D83), I70 (≠ V84), Y107 (≠ I130), G110 (= G133), G111 (= G134), E114 (≠ G137), P133 (= P156), E134 (= E157), L137 (≠ I160), G142 (≠ D165)
Sites not aligning to the query:
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
31% identity, 45% coverage: 30:200/383 of query aligns to 15:176/258 of 1ey3A
- active site: A66 (≠ G82), M71 (≠ L87), S81 (≠ R111), L85 (= L115), G109 (= G134), E112 (≠ G137), P131 (= P156), E132 (= E157), T137 (≠ L162), P139 (= P164), G140 (≠ D165)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (= K39), L26 (= L41), A28 (= A43), A64 (= A80), G65 (= G81), A66 (≠ G82), D67 (= D83), I68 (≠ V84), L85 (= L115), W88 (vs. gap), G109 (= G134), P131 (= P156), L135 (≠ I160), G140 (≠ D165)
Sites not aligning to the query:
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
30% identity, 45% coverage: 30:200/383 of query aligns to 16:172/254 of 2dubA
- active site: A67 (≠ G82), M72 (≠ L87), S82 (≠ R111), G105 (= G134), E108 (≠ G137), P127 (= P156), E128 (= E157), T133 (≠ L162), P135 (= P164), G136 (≠ D165)
- binding octanoyl-coenzyme a: K25 (= K39), A26 (= A40), L27 (= L41), A29 (= A43), A65 (= A80), A67 (≠ G82), D68 (= D83), I69 (≠ V84), K70 (≠ R85), G105 (= G134), E108 (≠ G137), P127 (= P156), E128 (= E157), G136 (≠ D165), A137 (≠ V166)
Sites not aligning to the query:
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
31% identity, 45% coverage: 30:200/383 of query aligns to 17:176/258 of 1mj3A
- active site: A68 (≠ G82), M73 (≠ L87), S83 (≠ R111), L85 (= L112), G109 (= G134), E112 (≠ G137), P131 (= P156), E132 (= E157), T137 (≠ L162), P139 (= P164), G140 (≠ D165)
- binding hexanoyl-coenzyme a: K26 (= K39), A27 (= A40), L28 (= L41), A30 (= A43), A66 (= A80), G67 (= G81), A68 (≠ G82), D69 (= D83), I70 (≠ V84), G109 (= G134), P131 (= P156), E132 (= E157), L135 (≠ I160), G140 (≠ D165)
Sites not aligning to the query:
O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
30% identity, 45% coverage: 32:205/383 of query aligns to 20:189/266 of O53561
- K135 (≠ R152) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
- 135:142 (vs. 152:159, 13% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
- K142 (≠ T159) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.
6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
32% identity, 39% coverage: 30:177/383 of query aligns to 10:144/692 of 6iunB
Sites not aligning to the query:
- active site: 248, 407, 428, 440, 478
- binding nicotinamide-adenine-dinucleotide: 300, 301, 302, 321, 322, 365, 377, 378, 380, 384, 388, 405, 407
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
28% identity, 44% coverage: 34:203/383 of query aligns to 19:182/261 of 5jbxB
- active site: A67 (≠ G82), R72 (≠ A90), L84 (≠ F106), R88 (≠ Y110), G112 (= G134), E115 (≠ G137), T134 (≠ P156), E135 (= E157), I140 (≠ L162), P142 (= P164), G143 (≠ D165)
- binding coenzyme a: S24 (≠ K39), R25 (≠ A40), R26 (≠ L41), A28 (= A43), A65 (= A80), D68 (= D83), L69 (= L87), K70 (≠ Y88), L110 (≠ M132), G111 (= G133), T134 (≠ P156), E135 (= E157), L138 (≠ I160), R168 (≠ H189)
Sites not aligning to the query:
6eqoA Tri-functional propionyl-coa synthase of erythrobacter sp. Nap1 with bound NADP+ and phosphomethylphosphonic acid adenylate ester (see paper)
30% identity, 42% coverage: 25:185/383 of query aligns to 862:1016/1804 of 6eqoA
Sites not aligning to the query:
- binding phosphomethylphosphonic acid adenylate ester: 456, 458, 535, 536, 537, 538, 558, 559, 560, 561, 562, 688, 714
- binding nadp nicotinamide-adenine-dinucleotide phosphate: 1261, 1265, 1379, 1400, 1403, 1404, 1405, 1424, 1425, 1429, 1444, 1492, 1493, 1497, 1514, 1517, 1713, 1730, 1731, 1774
1jxzB Structure of the h90q mutant of 4-chlorobenzoyl-coenzyme a dehalogenase complexed with 4-hydroxybenzoyl-coenzyme a (product) (see paper)
25% identity, 47% coverage: 30:209/383 of query aligns to 13:190/269 of 1jxzB
- active site: C61 (= C79), F64 (≠ G82), I69 (≠ L87), A86 (≠ K103), Q90 (≠ E107), G113 (= G133), G114 (= G134), G117 (= G137), A136 (≠ P156), W137 (≠ E157), I142 (≠ L162), N144 (≠ P164), D145 (= D165)
- binding 4-hydroxybenzoyl coenzyme a: R22 (≠ K39), H23 (≠ A40), R24 (≠ L41), A62 (= A80), F64 (≠ G82), Y65 (≠ D83), L66 (≠ V84), R67 (= R85), W89 (≠ F106), G113 (= G133), A136 (≠ P156), W137 (≠ E157), I142 (≠ L162), D145 (= D165), T146 (≠ V166)
- binding calcium ion: G49 (≠ A66)
Sites not aligning to the query:
Query Sequence
>200846 FitnessBrowser__MR1:200846
MTNLVDKAAHSFATQNVVFQTLATASGKLVGVVTLNVEKALNALDLDMVRAMTVQLNLWK
KDPLIACVVLDGSGEKAFCAGGDVRALYHASVAAKGQVTEVAKVFFEEEYRLDYLLHTYG
KPVLVWGDGIVMGGGLGLMAGASHKVVTETSRIAMPEVTIGLYPDVGGSYFLNRMPGKMG
LFLGLTAYHMNAADACYVGLADHYLNRDDKELMFDAMATLDWSDSPALNHQRLDTMINEL
SNQVDIPKGDSVLAESQEMIDRLMAGSLTDIVTRMSTLSTDEAWLSKACATMLAGSPISW
HLAYIQTQLGTKLSLAQCFKWELTVSVNVCAKGDFCEGVRALLIDKDKQPKWQFADVQSV
PNSVIEDILTSPWGEEHPLSQLS
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory