SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 201408 SO2258 lipoprotein releasing system ATP-binding protein LolD (NCBI ptt file) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
58% identity, 97% coverage: 1:223/230 of query aligns to 1:223/233 of P75957

query
sites
P75957

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G42 (= G42) mutation to D: Loss of lipoprotein release when overexpressed.

7mdyC Lolcde nucleotide-bound
58% identity, 96% coverage: 4:223/230 of query aligns to 1:220/226 of 7mdyC

query
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7mdyC

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binding adp orthovanadate: Y12 (= Y15), G42 (= G45), S43 (= S46), G44 (= G47), K45 (= K48), S46 (= S49), T47 (= T50), Q91 (= Q94), H138 (= H141), E142 (= E145), S144 (= S147), G145 (= G148), G146 (= G149), E168 (= E171), N172 (= N175), H201 (= H204)
binding magnesium ion: S46 (= S49), Q91 (= Q94)

7arlD Lolcde in complex with lipoprotein and adp (see paper)
58% identity, 96% coverage: 4:223/230 of query aligns to 1:220/222 of 7arlD

query
sites
7arlD

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binding adenosine-5'-diphosphate: G42 (= G45), G44 (= G47), K45 (= K48), S46 (= S49)

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
58% identity, 96% coverage: 4:223/230 of query aligns to 3:222/229 of 7v8iD

query
sites
7v8iD

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binding phosphoaminophosphonic acid-adenylate ester: V23 (= V24), S43 (= S44), G44 (= G45), G46 (= G47), K47 (= K48), S48 (= S49), T49 (= T50), Q93 (= Q94), R137 (= R138), H140 (= H141), E144 (= E145), S146 (= S147), G148 (= G149), E149 (= E150), H203 (= H204)
binding magnesium ion: S48 (= S49), Q93 (= Q94)

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
42% identity, 98% coverage: 5:230/230 of query aligns to 3:226/226 of 5xu1B

query
sites
5xu1B

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binding magnesium ion: G45 (= G47), K46 (= K48), S47 (= S49)

2pclA Crystal structure of abc transporter with complex (aq_297) from aquifex aeolicus vf5
48% identity, 87% coverage: 23:221/230 of query aligns to 17:214/223 of 2pclA

query
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2pclA

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R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

I

A

N

N

K

E

P

P

K

I

L

L

V

L

L

F

A

A

D

D

E
|
E

P
|
P

T
|
T

G

G

N

N

L

L

D

D

A

S

K

A

S

N

G

T

E

K

A

R

V

V

Y

M

E

D

L

I

I

F

R

L

E

K

L

I

A

-

N

N

Q

E

L

G

G

G

T

T

A

S

F

I

V

V

V

M

V

V

T
|
T

H

H

D

E

P

R

K

E

L

L

A

A

A

E

R

L

M

T

D

H

R

R

Q

T

L

L

T

E

M

M

K

K

N

D

G

G

binding magnesium ion: E165 (= E171), P166 (= P172), T167 (= T173), T196 (= T203)

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
45% identity, 94% coverage: 5:221/230 of query aligns to 4:219/648 of P75831

query
sites
P75831

L

L

Q

E

V

L

Q

K

A

D

V

I

S

R

K

R

S

S

Y

Y

H

P

D

A

G

G

D

D

V

E

T

Q

T

V

Q

E

V

V

L

L

S

K

D

G

V

I

D

S

L

L

Q

D

V

I

F

Y

K

A

G

G

E

E

Q

M

L

V

A

A

I

I

V

V

G

G

T

A

S

S

G

G

S

S

G

G

K
|
K

S

S

T

T

L

L

L

M

H

N

I

I

M

L

G

G

T

C

L

L

D

D

K

K

P

A

S

T

S

S

G

G

K

T

V

Y

L

R

L

V

A

A

G

G

E

Q

D

D

L

V

Y

A

Q

T

V

L

S

D

S

A

A

D

R

A

Q

L

A

A

Q

Q

I

L

R

R

N

R

Q

E

D

H

L

F

G

G

F

F

I

I

Y

F

Q

Q

F

R

H

Y

H

H

L

L

P

S

E

H

F

L

T

T

A

A

L

E

E

Q

N

N

V

V

A

E

M

V

P

P

A

A

F

V

I

Y

Q

A

G

G

R

L

D

E

R

R

T

K

L

Q

A

R

Q

L

A

L

D

R

A

A

K

Q

V

E

L

L

E

Q

R

R

V

L

G

G

L

L

G

E

H

D

R

R

M

T

S

E

H

Y

I

Y

P

P

A

A

E

Q

L

L

S

S

G

G

E

Q

R

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

I

M

N

N

K

G

P

G

K

Q

L

V

V

I

L

L

A

A

D
|
D

E

E

P

P

T

T

G

G

N

A

L

L

D

D

A

S

K

H

S

S

G

G

E

E

A

E

V

V

Y

M

E

A

L

I

I

L

R

H

E

Q

L

L

A

R

N

D

Q

R

L

-

G

G

T

H

A

T

F

V

V

I

V

V

T

T

H

H

D

D

P

P

K

Q

L

V

A

A

R

Q

M

A

D

E

R

R

Q

V

L

I

T

E

M

I

K

R

N

D

G

G

K47 (= K48) mutation to L: Lack of activity.
D169 (= D170) mutation to N: Lack of activity.

5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
40% identity, 94% coverage: 5:221/230 of query aligns to 3:218/615 of 5lilA

query
sites
5lilA

L

I

Q

E

V

I

Q

K

A

Q

V

L

S

N

K

R

S

Y

Y
x
F

H

G

D

E

G

G

D

E

V

N

T

R

T

V

Q

H

V
|
V

L

L

S

K

D

D

V

I

D

S

L

L

Q

S

V

I

F

E

K

R

G

G

E

D

Q

F

L

V

A

A

I

I

V

M

G

G

T

Q

S
|
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

M

H

N

I

I

M

I

G

G

T

C

L

L

D

D

K

T

P

A

S

T

S

G

G

G

K

S

V

S

L

K

L

I

A

D

G

G

E

K

D

E

L

T

Y

I

Q

E

V

L

S

T

S

N

A

D

R

Q

Q

L

A

S

Q

D

I

L

R

R

N

S

Q

Q

D

K

L

F

G

G

F

F

I

I

Y

F

Q
|
Q

F

R

H

Y

H

N

L

L

P

S

E

S

F

L

T

T

A

A

L

A

E

E

N

N

V

V

A

A

M

L

P

P

A

A

F

I

I

Y

Q

A

G

G

R

M

D

P

R

Q

T

S

L

Q

A

R

Q

L

A

E

D

R

A

A

K

K

V

Q

L

L

E

E

R

K

V

L

G

G

L

L

G

G

H

D

R
x
K

M

W

S

Q

H

N

I

K

P

P

A

N

E
x
Q

L

L

S
|
S

G

G

G
|
G

E
x
Q

R

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

I

M

N

N

K

G

P

G

K

E

L

I

V

I

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

A

L

L

D

D

A

S

K

H

S

S

G

G

E

E

A

N

V

V

Y

M

E

E

L

I

I

L

R

R

E

Q

L

L

A

H

N

E

Q

E

L

-

G

G

T

H

A

T

F

I

V

I

V

M

V

V

T

T

H

H

D

D

P

K

K

H

L

I

A

A

R

S

M

A

D

N

R

R

Q

I

L

I

T

E

M

I

K

K

N

D

G

G

binding adenosine-5'-triphosphate: F13 (≠ Y15), V22 (= V24), S42 (= S44), G43 (= G45), G45 (= G47), K46 (= K48), S47 (= S49), T48 (= T50), Q92 (= Q94), K136 (≠ R138), Q143 (≠ E145), S145 (= S147), G147 (= G149), Q148 (≠ E150)
binding magnesium ion: S47 (= S49), Q92 (= Q94)

5lj7A Structure of aggregatibacter actinomycetemcomitans macb bound to atp (p21) (see paper)
40% identity, 94% coverage: 5:221/230 of query aligns to 3:218/592 of 5lj7A

query
sites
5lj7A

L

I

Q

E

V

I

Q

K

A

Q

V

L

S

N

K

R

S

Y

Y

F

H

G

D

E

G

G

D

E

V

N

T

R

T

V

Q

H

V
|
V

L

L

S

K

D

D

V

I

D

S

L

L

Q

S

V

I

F

E

K

R

G

G

E

D

Q

F

L

V

A

A

I

I

V

M

G

G

T

Q

S
|
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

M

H

N

I

I

M

I

G

G

T

C

L

L

D

D

K

T

P

A

S

T

S

G

G

G

K

S

V

S

L

K

L

I

A

D

G

G

E

K

D

E

L

T

Y

I

Q

E

V

L

S

T

S

N

A

D

R

Q

Q

L

A

S

Q

D

I

L

R

R

N

S

Q

Q

D

K

L

F

G

G

F

F

I

I

Y

F

Q
|
Q

F

R

H

Y

H

N

L

L

P

S

E

S

F

L

T

T

A

A

L

A

E

E

N

N

V

V

A

A

M

L

P

P

A

A

F

I

I

Y

Q

A

G

G

R

M

D

P

R

Q

T

S

L

Q

A

R

Q

L

A

E

D

R

A

A

K

K

V

Q

L

L

E

E

R

K

V

L

G

G

L

L

G

G

H

D

R
x
K

M

W

S

Q

H

N

I

K

P

P

A

N

E
x
Q

L

L

S
|
S

G

G

G
|
G

E
x
Q

R

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

I

M

N

N

K

G

P

G

K

E

L

I

V

I

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

A

L

L

D

D

A

S

K

H

S

S

G

G

E

E

A

N

V

V

Y

M

E

E

L

I

I

L

R

R

E

Q

L

L

A

H

N

E

Q

E

L

-

G

G

T

H

A

T

F

I

V

I

V

M

V

V

T

T

H

H

D

D

P

K

K

H

L

I

A

A

R

S

M

A

D

N

R

R

Q

I

L

I

T

E

M

I

K

K

N

D

G

G

binding adenosine-5'-triphosphate: V22 (= V24), S42 (= S44), G43 (= G45), G45 (= G47), K46 (= K48), S47 (= S49), T48 (= T50), Q92 (= Q94), K136 (≠ R138), Q143 (≠ E145), S145 (= S147), G147 (= G149), Q148 (≠ E150)
binding magnesium ion: S47 (= S49), Q92 (= Q94)

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
42% identity, 94% coverage: 5:221/230 of query aligns to 4:219/650 of 5ws4A

query
sites
5ws4A

L

L

Q

E

V

V

Q

S

A

N

V

L

S

V

K

R

S

E

Y

F

H

P

D

A

G

G

D

E

V

S

T

T

T

I

Q

Q

V

I

L

L

S

K

D

G

V

I

D

D

L

L

Q

T

V

I

F

Y

K

E

G

G

E

E

Q

L

L

V

A

A

I

I

V

V

G

G

T

Q

S
|
S

G
|
G

S

S

G

G

K

K

S
|
S

T

T

L

L

L

M

H

N

I

I

M

L

G

G

T

C

L

L

D

D

K

R

P

P

S

T

S

S

G

G

K

S

V

Y

L

K

L

V

A

N

G

G

E

Q

D

E

L

T

Y

G

Q

K

V

L

S

E

S

P

A

D

R

Q

Q

L

A

A

Q

Q

I

L

R

R

N

R

Q

E

D

Y

L

F

G

G

F

F

I

I

Y

F

Q

Q

F

R

H

Y

H

H

L

L

P

G

E

D

F

L

T

S

A

A

L

E

E

G

N

N

V

V

A

E

M

V

P

P

A

A

F

V

I

Y

Q

A

G

G

R

V

D

T

R

P

T

A

L

D

A

R

Q

K

A

Q

D

R

A

A

K

T

V

A

L

L

E

T

R

E

V

L

G

G

L

L

G

G

H

T

R

K

M

T

S

Q

H

N

I

R

P

P

A

S

E

Q

L

L

S

S

G

G

E

Q

R

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

I

M

N

N

K

G

P

G

K

D

L

V

V

I

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

A

L

L

D

D

A

S

K

H

S

S

G

G

E

V

A

E

V

V

Y

M

E

R

L

I

I

L

R

R

E

E

L

L

A

-

N

N

Q

A

L

A

G

G

T

H

A

T

F

I

V

I

V

L

V

V

T

T

H

H

D

D

P

M

K

Q

L

V

A

A

A

K

R

N

M

A

D

T

R

R

Q

I

L

I

T

E

M

I

K

S

N

D

G

G

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S43 (= S44), G44 (= G45), S48 (= S49)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
39% identity, 97% coverage: 5:228/230 of query aligns to 1:228/232 of 1f3oA

query
sites
1f3oA

L

M

L

I

Q

K

V

L

Q

K

A

N

V

V

S

T

K

K

S

T

Y
|
Y

H

K

D

M

G

G

D

E

V

E

T

I

Q

Y

V

A

L

L

S

K

D

N

V

V

D

N

L

L

Q

N

V

I

F

K

K

E

G

G

E

E

Q

F

L

V

A

S

I

I

V

M

G

G

T

P

S

S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

H

N

I

I

M

I

G

G

T

C

L

L

D

D

K

K

P

P

S

T

S

E

G

G

K

E

V

V

L

Y

L

I

A

D

G

N

E

I

D

K

L

T

Y

N

Q

D

V

L

S

D

A

D

R

E

Q

L

A

T

Q

K

I

I

R

R

N

R

Q

D

D

K

L

I

G

G

F

F

I

V

Y

F

Q

Q

F

Q

H

F

H

N

L

L

L

I

P

P

E

L

F

L

T

T

A

A

L

L

E

E

N

N

V

V

A

E

M

L

P

P

A

L

F

I

I

F

Q

K

G

Y

R

R

D

G

R

A

T

M

L

S

A

G

Q

E

A

E

D

R

A

R

K

K

V

R

L

A

L

L

E

E

R

C

V

L

G

K

L

M

G

A

H

E

R

L

-

E

-

R

-

F

M

A

S

N

H

H

I

K

P

P

A

N

E

Q

L

L

S

S

G

G

E

Q

R

Q

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

I

A

N

N

K

N

P

P

K

P

L

I

V

I

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

A

L

L

D

D

A

S

K

K

S

T

G

G

E

E

A

K

V

I

Y

M

E

Q

L

L

I

L

R

K

E

K

L

L

A

N

N

E

Q

E

L

D

G

G

T

K

A

T

F

V

V

V

T

T

H

H

D

D

P

I

K

N

L

V

A

A

A

R

R

F

M

G

D

E

R

R

Q

I

L

I

T

Y

M

L

K

K

N

D

G

G

Y

E

L

V

Q

E

V

R

P

E

E

E

S

K

binding adenosine-5'-diphosphate: Y11 (= Y15), G41 (= G45), G43 (= G47), K44 (= K48), S45 (= S49), T46 (= T50)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
38% identity, 97% coverage: 5:228/230 of query aligns to 1:228/230 of 1l2tA

query
sites
1l2tA

L

M

L

I

Q

K

V

L

Q

K

A

N

V

V

S

T

K

K

S

T

Y
|
Y

H

K

D

M

G

G

D

E

V

E

T

I

Q

Y

V

A

L

L

S

K

D

N

V

V

D

N

L

L

Q

N

V

I

F

K

K

E

G

G

E

E

Q

F

L

V

A

S

I

I

V

M

G

G

T

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

H

N

I

I

M

I

G

G

T

C

L

L

D

D

K

K

P

P

S

T

S

E

G

G

K

E

V

V

L

Y

L

I

A

D

G

N

E

I

D

K

L

T

Y

N

Q

D

V

L

S

D

A

D

R

E

Q

L

A

T

Q

K

I

I

R

R

N

R

Q

D

D

K

L

I

G

G

F

F

I

V

Y

F

Q

Q

F

Q

H

F

H

N

L

L

L

I

P

P

E

L

F

L

T

T

A

A

L

L

E

E

N

N

V

V

A

E

M

L

P

P

A

L

F

I

I

F

Q

K

G

Y

R

R

D

G

R

A

T

M

L

S

A

G

Q

E

A

E

D

R

A

R

K

K

V

R

L

A

L

L

E

E

R

C

V

L

G

K

L

M

G

A

H

E

R

L

-

E

-

R

-
x
F

M

A

S

N

H

H

I

K

P

P

A

N

E
x
Q

L

L

S
|
S

G

G

G
|
G

E
x
Q

R

Q

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

I

A

N

N

K

N

P

P

K

P

L

I

V

I

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

A

L

L

D

D

A

S

K

K

S

T

G

G

E

E

A

K

V

I

Y

M

E

Q

L

L

I

L

R

K

E

K

L

L

A

N

N

E

Q

E

L

D

G

G

T

K

A

T

F

V

V

V

T

T

H
|
H

D

D

P

I

K

N

L

V

A

A

A

R

R

F

M

G

D

E

R

R

Q

I

L

I

T

Y

M

L

K

K

N

D

G

G

Y

E

L

V

Q

E

V

R

P

E

E

E

S

K

binding adenosine-5'-triphosphate: Y11 (= Y15), S40 (= S44), G41 (= G45), S42 (= S46), G43 (= G47), K44 (= K48), S45 (= S49), T46 (= T50), F138 (vs. gap), Q145 (≠ E145), S147 (= S147), G149 (= G149), Q150 (≠ E150), H204 (= H204)

8w6iD Cryo-em structure of escherichia coli str k12 ftsex complex with atp- gamma-s in peptidisc
39% identity, 96% coverage: 5:224/230 of query aligns to 1:216/219 of 8w6iD

query
sites
8w6iD

L

M

L

I

Q

R

V

F

Q

E

A

H

V

V

S

S

K

K

S

A

Y
|
Y

H

L

D

G

G

G

D

-

V

-

T

-

T

R

Q

Q

V

A

L

L

S

Q

D

G

V

V

D

T

L

F

Q

H

V

M

F

Q

K

P

G

G

E

E

Q

M

L

A

A

F

I

L

V

T

G

G

T

H

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

H

K

I

L

M

I

G

C

T

G

L

I

D

E

K

R

P

P

S

S

S

A

G

G

K

K

V

I

L

W

L

F

A

S

G

G

E

H

D

D

L

I

Y

T

Q

R

V

L

S

K

S

N

A

R

R

E

Q

V

A

P

Q

F

I

L

R

R

N

R

Q

Q

D

-

L

I

G

G

F

M

I

I

Y

F

Q
|
Q

F

D

H

H

L

L

P

M

E

D

F

R

T

T

A

V

L

Y

E

D

N

N

V

V

A

A

M

I

P

P

A

L

F

I

I

I

Q

A

G

G

R

A

D

S

R

G

T

D

L

D

A

I

Q

R

A

R

D

R

A

V

K

S

V

A

L

A

L

L

E

D

R

K

V

V

G

G

L

L

G

L

H

D

R
x
K

M

A

S

K

H

N

I

F

P

P

A

I

E
x
Q

L

L

S
|
S

G
|
G

E
|
E

R

Q

Q

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

L

V

I

V

N

N

K

K

P

P

K

A

L

V

V

L

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

A

D

K

A

S

L

G

S

E

E

A

G

V

I

Y

L

E

R

L

L

I

F

R

E

E

E

L

F

A

-

N

N

Q

R

L

V

G

G

T

V

A

T

F

V

V

L

V

M

V

A

T

T

H
|
H

D

D

P

I

K

N

L

L

A

I

A

S

R

R

M

R

D

S

-

Y

R

R

Q

M

L

L

T

T

M

L

K

S

N

D

G

G

Y

H

L

L

Q

H

binding phosphothiophosphoric acid-adenylate ester: Y11 (= Y15), S37 (= S44), G38 (= G45), A39 (≠ S46), G40 (= G47), K41 (= K48), S42 (= S49), T43 (= T50), Q86 (= Q94), K130 (≠ R138), Q137 (≠ E145), S139 (= S147), G140 (= G148), G141 (= G149), E142 (= E150), H195 (= H204)

P0A9R7 Cell division ATP-binding protein FtsE from Escherichia coli (strain K12) (see paper)
39% identity, 96% coverage: 5:224/230 of query aligns to 1:216/222 of P0A9R7

query
sites
P0A9R7

L

M

L

I

Q

R

V

F

Q

E

A

H

V

V

S

S

K

K

S

A

Y

Y

H

L

D

G

G

G

D

-

V

-

T

-

T

R

Q

Q

V

A

L

L

S

Q

D

G

V

V

D

T

L

F

Q

H

V

M

F

Q

K

P

G

G

E

E

Q

M

L

A

A

F

I

L

V

T

G

G

T

H

S

S

G

G

S

A

G

G

K
|
K

S

S

T

T

L

L

H

K

I

L

M

I

G
x
C

T

G

L

I

D

E

K

R

P

P

S

S

S

A

G

G

K

K

V

I

L

W

L

F

A

S

G

G

E

H

D

D

L

I

Y

T

Q

R

V

L

S

K

S

N

A

R

R

E

Q

V

A

P

Q

F

I

L

R

R

N

R

Q

Q

D

-

L

I

G

G

F

M

I

I

Y

F

Q

Q

F

D

H

H

L

L

P

M

E

D

F

R

T

T

A

V

L

Y

E

D

N

N

V

V

A

A

M

I

P

P

A

L

F

I

I

I

Q

A

G

G

R

A

D

S

R

G

T

D

L

D

A

I

Q

R

A

R

D

R

A

V

K

S

V

A

L

A

L

L

E

D

R

K

V

V

G

G

L

L

G

L

H

D

R

K

M

A

S

K

H

N

I

F

P

P

A

I

E

Q

L

L

S

S

G

G

E

E

R

Q

Q

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

L

V

I

V

N

N

K

K

P

P

K

A

L

V

V

L

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

A

D

K

A

S

L

G

S

E

E

A

G

V

I

Y

L

E

R

L

L

I

F

R

E

E

E

L

F

A

-

N

N

Q

R

L

V

G

G

T

V

A

T

F

V

V

L

V

M

V

A

T

T

H

H

D

D

P

I

K

N

L

L

A

I

A

S

R

R

M

R

D

S

-

Y

R

R

Q

M

L

L

T

T

M

L

K

S

N

D

G

G

Y

H

L

L

Q

H

K41 (= K48) mutation to R: Does not bind ATP.
C49 (≠ G56) mutation to A: Prevents dimer formation. Does not alter ATP-binding.

8i6rB Cryo-em structure of pseudomonas aeruginosa ftse(e163q)x/envc complex with atp in peptidisc (see paper)
38% identity, 95% coverage: 5:223/230 of query aligns to 1:215/222 of 8i6rB

query
sites
8i6rB

L

M

L

I

Q

R

V

F

Q

E

A

Q

V

V

S

G

K

K

S

R

Y
|
Y

H

P

D

N

G

G

D
x
H

V

V

T

G

T

-

Q

-

V

-

L

L

S

H

D

E

V

V

D

S

L

F

Q

R

V

V

F

H

K

R

G

G

E

E

Q

I

L

L

A

F

I

V

V

T

G

G

T

H

S
|
S

G

G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

H

R

I

L

M

I

G

L

T

A

L

M

D

E

K

R

P

P

S

T

S

S

G

G

K

K

V

L

L

L

A

G

G

G

E

Q

D

D

L

L

Y

G

Q

R

V

I

S

T

A

A

R

Q

Q

I

A

P

Q

F

I

L

R

R

N

R

Q

Q

D

-

L

I

G

G

F

V

I

V

Y

F

Q

Q

F

N

H

H

H

Q

L

L

P

T

E

D

F

R

T

T

A

V

L

A

E

D

N

N

V

I

A

A

M

L

P

P

A

L

F

Q

I

I

Q

L

G

G

R

M

D

P

R

K

T

P

L

E

A

I

Q

A

A

K

D

R

A

V

K

A

V

S

L

A

L

L

E

E

R

R

V

V

G

N

L

L

G

K

H

E

R

K

M

G

S

E

H

A

I

L

P

P

A

S

E
x
D

L

L

S
|
S

G
x
T

G
|
G

E
x
Q

R

Q

Q

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

L

I

I

V

N

H

K

Q

P

P

K

A

L

L

V

L

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

N

L

L

D

D

A

P

K

R

S

L

G

A

E

S

A

E

V

I

Y

M

E

G

L

V

I

F

R

E

E

D

L

I

A

-

N

N

Q

R

L

L

G

G

T

T

A

T

F

V

V

L

V

I

V

A

T

S

H

H

D

D

P

L

K

A

L

L

A

I

A

A

R

R

M

M

-

R

D

H

R

R

Q

M

L

L

T

T

M

L

K

Q

N

R

G

G

Y

R

L

I

binding adenosine-5'-triphosphate: Y11 (= Y15), H15 (≠ D19), S37 (= S44), A39 (≠ S46), G40 (= G47), K41 (= K48), S42 (= S49), T43 (= T50), D137 (≠ E145), S139 (= S147), T140 (≠ G148), G141 (= G149), Q142 (≠ E150)
binding magnesium ion: K41 (= K48), S42 (= S49)

8hd0A Cell divisome spg hydrolysis machinery ftsex-envc
38% identity, 96% coverage: 5:224/230 of query aligns to 1:216/218 of 8hd0A

query
sites
8hd0A

L

M

L

I

Q

R

V

F

Q

E

A

H

V

V

S

S

K

K

S

A

Y
|
Y

H

L

D

G

G

G

D

-

V

-

T

-

T
x
R

Q

Q

V
x
A

L

L

S

Q

D

G

V

V

D

T

L

F

Q

H

V

M

F

Q

K

P

G

G

E

E

Q

M

L

A

A

F

I

L

V

T

G

G

T

H

S

S

G

G

S

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

H

K

I

L

M

I

G

C

T

G

L

I

D

E

K

R

P

P

S

S

S

A

G

G

K

K

V

I

L

W

L

F

A

S

G

G

E

H

D

D

L

I

Y

T

Q

R

V

L

S

K

S

N

A

R

R

E

Q

V

A

P

Q

F

I

L

R

R

N

R

Q

Q

D

-

L

I

G

G

F

M

I

I

Y

F

Q

Q

F

D

H

H

L

L

P

M

E

D

F

R

T

T

A

V

L

Y

E

D

N

N

V

V

A

A

M

I

P

P

A

L

F

I

I

I

Q

A

G

G

R

A

D

S

R

G

T

D

L

D

A

I

Q

R

A

R

D

R

A

V

K

S

V

A

L

A

L

L

E

D

R

K

V

V

G

G

L

L

G

L

H

D

R

K

M

A

S

K

H

N

I

F

P

P

A

I

E

Q

L

L

S
|
S

G

G

G
|
G

E

E

R

Q

Q

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

L

V

I

V

N

N

K

K

P

P

K

A

L

V

V

L

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

N

L

L

D

D

A

D

K

A

S

L

G

S

E

E

A

G

V

I

Y

L

E

R

L

L

I

F

R

E

E

E

L

F

A

-

N

N

Q

R

L

V

G

G

T

V

A

T

F

V

V

L

V

M

V

A

T

T

H

H

D

D

P

I

K

N

L

L

A

I

A

S

R

R

M

R

D

S

-

Y

R

R

Q

M

L

L

T

T

M

L

K

S

N

D

G

G

Y

H

L

L

Q

H

binding adenosine-5'-triphosphate: Y11 (= Y15), R15 (≠ T22), A17 (≠ V24), G40 (= G47), K41 (= K48), S42 (= S49), T43 (= T50), S139 (= S147), G141 (= G149)

8g4cB Bceabs atpgs high res tm (see paper)
34% identity, 99% coverage: 4:230/230 of query aligns to 2:230/248 of 8g4cB

query
sites
8g4cB

V

V

L

I

L

L

Q

E

V

A

Q

N

A

K

V

I

S

R

K

K

S

S

Y
|
Y

H

G

D

N

G

K

D

L

V

N

T

K

T

Q

Q

E

V

V

L

L

S

K

D

G

V

I

D

D

L

I

Q

H

V

I

F

E

K

K

G

G

E

E

Q

F

L

V

A

S

I

I

V

M

G

G

T

A

S
|
S

G

G

S
|
S

G

G

K

K

S
x
T

T

T

L

L

H

N

I

V

M

L

G

S

T

S

L

I

D

D

K

Q

P

V

S

S

S

H

G

G

K

T

V

I

L

H

L

I

A

N

G

G

E

N

D

D

L

M

Y

T

Q

A

V

M

S

K

S

E

A

K

R

Q

Q

L

A

A

Q

E

I

F

R

R

N

K

Q

Q

D

H

L

L

G

G

F

F

I

I

Y

F

Q

Q

F

D

H

Y

H

N

L

L

P

D

E

T

F

L

T

T

A

V

L

K

E

E

N

N

V

I

A

L

M

L

P

P

A

L

F

S

I

I

Q

T

G

K

R

L

D

S

R

K

T

K

L

E

A

A

Q

N

A

R

D

K

A

F

K

E

V

E

L

V

L

A

E

K

R

E

V

L

G

G

L

I

G

Y

H

E

R

L

M

R

S

D

H

K

I

Y

P

P

A

N

E

E

L

I

S

S

G

G

E

Q

R

K

Q

Q

R

R

V

T

A

S

I

A

A

G

R

R

A

A

L

F

I

I

N

H

K

D

P

P

K

S

L

I

V

I

L

F

A

A

D
|
D

E

E

P

P

T

T

G

G

N

A

L

L

D

D

A

S

K

K

S

S

G

A

E

S

A

D

V

L

Y

L

E

N

L

K

I

L

R

S

E

Q

L

L

A

N

N

Q

Q

K

L

R

G

N

T

A

A

T

F

I

V

I

V

M

V

V

T

T

H

H

D

D

P

P

K

V

L

A

A

A

A

S

R

Y

M

C

D

G

R

R

Q

V

L

I

T

F

M

I

K

K

N

D

G

G

-

Q

-

M

Y

Y

L

T

Q

Q

V

L

P

N

E

K

S

G

A

G

Q

Q

binding phosphothiophosphoric acid-adenylate ester: Y13 (= Y15), S42 (= S44), S44 (= S46), T47 (≠ S49), D168 (= D170)

7tchB Bceab e169q variant atp-bound conformation (see paper)
33% identity, 99% coverage: 4:230/230 of query aligns to 1:229/245 of 7tchB

query
sites
7tchB

V

V

L

I

L

L

Q

E

V

A

Q

N

A

K

V

I

S

R

K

K

S

S

Y
|
Y

H

G

D

N

G

K

D

L

V

N

T

K

T
x
Q

Q

E

V
|
V

L

L

S

K

D

G

V

I

D

D

L

I

Q

H

V

I

F

E

K

K

G

G

E

E

Q

F

L

V

A

S

I

I

V

M

G

G

T

A

S
|
S

G
|
G

S
|
S

G

G

K
|
K

S
x
T

T

T

L

L

H

N

I

V

M

L

G

S

T

S

L

I

D

D

K

Q

P

V

S

S

S

H

G

G

K

T

V

I

L

H

L

I

A

N

G

G

E

N

D

D

L

M

Y

T

Q

A

V

M

S

K

S

E

A

K

R

Q

Q

L

A

A

Q

E

I

F

R

R

N

K

Q

Q

D

H

L

L

G

G

F

F

I

I

Y

F

Q

Q

F

D

H

Y

H

N

L

L

P

D

E

T

F

L

T

T

A

V

L

K

E

E

N

N

V

I

A

L

M

L

P

P

A

L

F

S

I

I

Q

T

G

K

R

L

D

S

R

K

T

K

L

E

A

A

Q

N

A

R

D

K

A

F

K

E

V

E

L

V

L

A

E

K

R

E

V

L

G

G

L

I

G

Y

H

E

R

L

M

R

S

D

H

K

I

Y

P

P

A

N

E
|
E

L

I

S

S

G
|
G

E

Q

R

K

Q

Q

R

R

V

T

A

S

I

A

A

G

R

R

A

A

L

F

I

I

N

H

K

D

P

P

K

S

L

I

V

I

L

F

A

A

D

D

E

Q

P

P

T

T

G

G

N

A

L

L

D

D

A

S

K

K

S

S

G

A

E

S

A

D

V

L

Y

L

E

N

L

K

I

L

R

S

E

Q

L

L

A

N

N

Q

Q

K

L

R

G

N

T

A

A

T

F

I

V

I

V

M

V

V

T

T

H

H

D

D

P

P

K

V

L

A

A

A

A

S

R

Y

M

C

D

G

R

R

Q

V

L

I

T

F

M

I

K

K

N

D

G

G

-

Q

-

M

Y

Y

L

T

Q

Q

V

L

P

N

E

K

S

G

A

G

Q

Q

binding adenosine-5'-triphosphate: Y12 (= Y15), Q19 (≠ T22), V21 (= V24), S41 (= S44), G42 (= G45), S43 (= S46), K45 (= K48), T46 (≠ S49), E142 (= E145), G145 (= G148), G146 (= G149)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
35% identity, 95% coverage: 5:223/230 of query aligns to 1:219/343 of P30750

query
sites
P30750

L

M

L

I

Q

K

V

L

Q

S

A

N

V

I

S

T

K

K

S

V

Y

F

H

H

D

Q

G

G

D

T

V

R

T

T

T

I

Q

Q

V

A

L

L

S

N

D

N

V

V

D

S

L

L

Q

H

V

V

F

P

K

A

G

G

E

Q

Q

I

L

Y

A

G

I

V

V

I

G

G

T

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

H

R

I

C

M

V

G

N

T

L

L

L

D

E

K

R

P

P

S

T

S

E

G

G

K

S

V

V

L

L

L

V

A

D

G

G

E

Q

D

E

L

L

Y

T

Q

T

V

L

S

S

S

E

A

S

R

E

Q

L

A

T

Q

K

I

A

R

R

N

R

Q

Q

D

-

L

I

G

G

F

M

I

I

Y

F

Q

Q

F

H

H

F

H

N

L

L

P

S

E

S

F

R

T

T

A

V

L

F

E

G

N

N

V

V

A

A

M

L

P

P

A

L

F

E

I

L

Q

D

G

N

R

T

D

P

R

K

T

D

L

E

A

V

Q

K

A

R

D

R

A

V

K

T

V

E

L

L

E

S

R

L

V

V

G

G

L

L

G

G

H

D

R

K

M

H

S

D

H

S

I

Y

P

P

A

S

E

N

L

L

S

S

G

G

E

Q

R

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

I

A

N

S

K

N

P

P

K

K

L

V

V

L

L

L

A

C

D

D

E
|
E

P

A

T

T

G

S

N

A

L

L

D

D

A

P

K

A

S

T

G

T

E

R

A

S

V

I

Y

L

E

E

L

L

I

L

R

K

E

D

L

I

A

N

N

R

Q

R

L

L

G

G

T

L

A

T

F

I

V

L

V

I

T

T

H

H

D

E

P

M

K

D

L

V

A

V

A

K

R

R

M

I

-

C

D

D

R

C

Q

V

L

A

T

V

M

I

K

S

N

N

G

G

Y

E

L

L

40:46 (vs. 44:50, 86% identical) binding
E166 (= E171) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

7w78A Heme exporter hrtba in complex with mg-amppnp (see paper)
41% identity, 94% coverage: 5:221/230 of query aligns to 4:213/218 of 7w78A

query
sites
7w78A

L

V

L

L

Q

S

V

I

Q

T

A

N

V

A

S

S

K

V

S

V

Y
|
Y

H

P

D

D

G

G

D

I

V

S

T

T

T

V

Q

T

V

A

L

L

S

D

D

S

V

A

D

N

L

V

Q

E

V

I

F

F

K

P

G

G

E

E

Q

L

L

V

A

A

I

I

V

V

G

G

T

E

S
|
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

H

S

I

I

M

A

G

G

T

F

L

L

D

Q

K

E

P

P

S

T

S

S

G

G

K

T

V

V

L

T

L

L

A

H

G

G

E

A

D

E

L

G

Y

L

Q

D

V

A

S

T

S

S

A

T

R

R

Q

R

A

E

Q

H

I

I

R

-

N

-

Q

-

D

-

L

-

G

G

F

F

I

V

Y

F

Q
|
Q

F

Q

H

P

H

N

L

L

P

G

E

S

F

L

T

T

A

A

L

R

E

E

N

Q

V

L

A

L

M

I

P

T

A

D

F

H

I

L

Q

R

G

G

R

I

D

K

R

P

T

R

L

K

A

D

Q

R

A

A

D

D

A

E

K

-

V

-

L

L

E

A

R

R

V

V

G

G

L

L

G

K

H

G

R

L

M

G

S

G

H

R

I

R

P

V

A

A

E

Q

L

L

S

S

G

G

E

Q

R

R

Q

Q

R

R

V

V

A

N

I

I

A

A

R

R

A

A

L

L

I

M

N

G

K

N

P

P

K

Q

L

L

V

L

L

L

A

A

D

D

E
|
E

P

P

T

T

G

S

N

A

L

L

D

D

A

A

K

R

S

L

G

S

E

K

A

E

V

I

Y

V

E

E

L

L

I

L

R

R

E

D

L

V

A

T

N

K

Q

E

L

F

G

A

T

L

A

A

F

T

V

L

V

M

V

V

T

T

H

H

D

D

P

R

K

S

L

Q

A

L

A

A

R

Y

M

A

D

D

R

R

Q

F

L

V

T

E

M

M

K

A

N

D

G

G

binding phosphoaminophosphonic acid-adenylate ester: Y14 (= Y15), S43 (= S44), G44 (= G45), G46 (= G47), K47 (= K48), S48 (= S49), T49 (= T50), Q88 (= Q94), E163 (= E171)
binding magnesium ion: S48 (= S49), Q88 (= Q94)

Query Sequence

>201408 SO2258 lipoprotein releasing system ATP-binding protein LolD (NCBI ptt file)
MQDVLLQVQAVSKSYHDGDVTTQVLSDVDLQVFKGEQLAIVGTSGSGKSTLLHIMGTLDK
PSSGKVLLAGEDLYQVSSARQAQIRNQDLGFIYQFHHLLPEFTALENVAMPAFIQGRDRT
LAQADAKVLLERVGLGHRMSHIPAELSGGERQRVAIARALINKPKLVLADEPTGNLDAKS
GEAVYELIRELANQLGTAFVVVTHDPKLAARMDRQLTMKNGYLQVPESAQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory