SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 201412 FitnessBrowser__MR1:201412 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7bw9A Crystal structure of serine acetyltransferase isoform 3 in complex with cysteine from entamoeba histolytica
43% identity, 64% coverage: 7:180/273 of query aligns to 103:271/280 of 7bw9A

query
sites
7bw9A

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binding cysteine: D114 (= D18), P115 (= P19), D179 (= D83), H180 (= H84), A206 (≠ G110), R214 (= R118), H215 (= H119)

6wyeA Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) (see paper)
43% identity, 62% coverage: 7:174/273 of query aligns to 82:253/261 of 6wyeA

query
sites
6wyeA

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binding (2S)-2-hydroxybutanedioic acid: D93 (= D18), P94 (= P19), D158 (= D83), H159 (= H84), G185 (= G110), R193 (= R118), H194 (= H119)

4n69A Soybean serine acetyltransferase complexed with serine (see paper)
47% identity, 59% coverage: 10:170/273 of query aligns to 83:243/243 of 4n69A

query
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4n69A

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binding serine: G183 (= G110), R191 (= R118), H192 (= H119)

4hzdA Crystal structure of serine acetyltransferase in complex with coenzyme a from brucella abortus strain s19 (see paper)
41% identity, 60% coverage: 7:170/273 of query aligns to 81:244/250 of 4hzdA

query
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4hzdA

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binding coenzyme a: H158 (= H84), G203 (= G129), A204 (= A130), A221 (≠ G147), A222 (≠ S148), T235 (= T161), A237 (≠ V163), I244 (≠ V170)
binding magnesium ion: S224 (= S150), A237 (≠ V163), G238 (= G164)

7ra4A Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) in complex with serine (see paper)
43% identity, 60% coverage: 7:170/273 of query aligns to 80:243/243 of 7ra4A

query
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7ra4A

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binding serine: D91 (= D18), P92 (= P19), D156 (= D83), H157 (= H84), R191 (= R118), H192 (= H119)

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
43% identity, 63% coverage: 3:173/273 of query aligns to 80:250/272 of 3gvdI

query
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3gvdI

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binding cysteine: D95 (= D18), P96 (= P19), D160 (= D83), G186 (= G109), G187 (= G110), R195 (= R118)

8i04A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with serine (see paper)
43% identity, 63% coverage: 3:173/273 of query aligns to 73:243/258 of 8i04A

query
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8i04A

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binding serine: D88 (= D18), P89 (= P19), D153 (= D83), H154 (= H84), G180 (= G110), R188 (= R118), H189 (= H119)

1ssqD Serine acetyltransferase- complex with cysteine (see paper)
43% identity, 59% coverage: 10:171/273 of query aligns to 80:241/257 of 1ssqD

query
sites
1ssqD

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D

I

I

E

Q

S

A

I

V

Y

R

H

H

R

R

D
|
D

P

P

A

A

A

V

R

E

S

L

A

W

F

S

E

T

I

P

L

L

V

L

N

Y

Y

L

P

K

G

G

M

F

H

H

A

A

I

I

W

Q

L

S

H

Y

R

R

I

I

S

T

H

H

K

Y

L

L

W

W

K

N

R

Q

D

N

W

R

R

K

L

S

M

L

A

A

R

L

C

Y

L

L

S

Q

T

N

F

Q

S

I

R

S

W

V

L

A

T

F

G

D

V

V

E

D

I

I

H

H

P

P

G

A

A

A

T

K

I

I

G

G

N

H

R

G

F

I

F

M

I

F

D
|
D

H
|
H

G

A

M

T

G

G

V

I

V

V

I

V

G

G

E

E

T

T

A

S

E

V

I

I

G

E

D

N

D

D

C

V

T

S

L

I

Y

L

H

Q

G

G

V

V

T

T

L

L

G

G

G

G

T

T

T

G

W

K

Q

E

A

S

G

G

K

D

R

R

H

H

P

P

T

K

L

V

G

R

N

E

N

G

V

V

V

M

V

I

G

G

A

A

G

G

A

A

K

K

I

I

L

L

G

G

P

N

I

I

T

E

M

V

H

G

D

K

G

Y

A

A

R

K

V

I

G

G

S

A

N

N

S

S

V

V

V

V

V

L

K

N

D

P

V

V

P

P

K

E

D

Y

T

A

T

T

V

A

V

A

G

G

I

V

P

P

G

A

R

R

V

I

V

V

A

S

binding cysteine: D88 (= D18), D153 (= D83), H154 (= H84)

8i09A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with butyl gallate (see paper)
43% identity, 63% coverage: 3:173/273 of query aligns to 76:246/246 of 8i09A

query
sites
8i09A

V

M

I

I

A

A

R

S

L

A

K

A

E

C

D

D

I

I

E

Q

S

A

I

V

Y

R

H

T

R

R

D
|
D

P

P

A

A

A

V

R

D

S

K

A

Y

F

S

E

T

I

P

L

L

V

L

N

Y

Y

L

P

K

G

G

M

F

H

H

A

A

I

L

W

Q

L

A

H

Y

R

R

I

I

S

G

H

H

K

W

L

L

W

W

K

N

R

K

D

G

W

R

R

R

L

A

M

L

A

A

R

I

C

F

L

L

S

Q

T

N

F

Q

S

V

R

S

W

V

L

S

T

F

G

Q

V

V

E

D

I

I

H

H

P

P

G

A

A

A

T

K

I

I

G

G

N

R

R

G

F

I

F

M

I

L

D
|
D

H
|
H

G

A

M

T

G

G

V

I

V

V

I

V

G
|
G

E

E

T

T

A

A

E

V

I

I

G

E

D

D

D

D

C

V

T

S

L

I

Y

L

H

Q

G

S

V

V

T

T

L

L

G

G

G
|
G

T
|
T

T

G

W

K

Q

T

A

S

G

G

K

D

R
|
R

H
|
H

P

P

T

K

L

I

G

R

N

E

N

G

V

V

V

M

V

I

G
|
G

A

A

G

G

A

A

K

K

I

I

L

L

G

G

P

N

I

I

T

E

M

V

H

G

D

R

G

G

A

A

R
x
K

V
x
I

G
|
G

S

A

N

G

S

S

V

V

V

V

V
x
L

K

Q

D

P

V

V

P

P

K

P

D

H

T

T

T

T

V

A

V
x
A

G

G

I

V

P

P

G

A

R

R

V

I

V

V

A

G

T

K

P

P

binding cysteine: D91 (= D18), D156 (= D83), H157 (= H84), G164 (= G91), R191 (= R118), H192 (= H119)
binding butyl 3,4,5-tris(oxidanyl)benzoate: G183 (= G110), T184 (= T111), G202 (= G129), K218 (≠ R145), I219 (≠ V146), G220 (= G147), L226 (≠ V153), A236 (≠ V163)

1t3dA Crystal structure of serine acetyltransferase from e.Coli at 2.2a (see paper)
42% identity, 63% coverage: 3:173/273 of query aligns to 77:247/262 of 1t3dA

query
sites
1t3dA

V

M

I

I

A

A

R

S

L

A

K

A

E

C

D

D

I

I

E

Q

S

A

I

V

Y

R

H

T

R

R

D
|
D

P
|
P

A

A

A

V

R

D

S

K

A

Y

F

S

E

T

I

P

L

L

V

L

N

Y

Y

L

P

K

G

G

M

F

H

H

A

A

I

L

W

Q

L

A

H

Y

R

R

I

I

S

G

H

H

K

W

L

L

W

W

K

N

R

Q

D

G

W

R

R

R

L

A

M

L

A

A

R

I

C

F

L

L

S

Q

T

N

F

Q

S

V

R

S

W

V

L

T

T

F

G

Q

V

V

E

D

I

I

H

H

P

P

G

A

A

A

T

K

I

I

G

G

N

R

R

G

F

I

F

M

I

L

D
|
D

H

H

G

A

M

T

G

G

V

I

V

V

I

V

G

G

E

E

T

T

A

A

E

V

I

I

G

E

D

N

D

D

C

V

T

S

L

I

Y

L

H

Q

G

S

V

V

T

T

L

L

G

G

G
|
G

T

T

T

G

W

K

Q

S

A

G

G

G

K

D

R
|
R

H
|
H

P

P

T

K

L

I

G

R

N

E

N

G

V

V

V

M

V

I

G

G

A

A

G

G

A

A

K

K

I

I

L

L

G

G

P

N

I

I

T

E

M

V

H

G

D

R

G

G

A

A

R

K

V

I

G

G

S

A

N

G

S

S

V

V

V

V

V

L

K

Q

D

P

V

V

P

P

K

P

D

H

T

T

T

T

V

A

V

A

G

G

I

V

P

P

G

A

R

R

V

I

V

V

A

G

T

K

P

P

binding cysteine: D92 (= D18), P93 (= P19), D157 (= D83), G184 (= G110), R192 (= R118), H193 (= H119)

8i06A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with coa (see paper)
43% identity, 62% coverage: 3:170/273 of query aligns to 77:244/244 of 8i06A

query
sites
8i06A

V

M

I

I

A

A

R

S

L

A

K

A

E

C

D

D

I

I

E

Q

S

A

I

V

Y

R

H

T

R

R

D

D

P
|
P

A

A

A

V

R

D

S

K

A

Y

F

S

E

T

I

P

L

L

V

L

N

Y

Y

L

P

K

G

G

M

F

H

H

A

A

I

L

W

Q

L

A

H

Y

R

R

I

I

S

G

H

H

K

W

L

L

W

W

K

N

R

K

D

G

W

R

R

R

L

A

M

L

A

A

R

I

C

F

L

L

S

Q

T

N

F

Q

S

V

R

S

W

V

L

S

T

F

G

Q

V

V

E

D

I

I

H

H

P

P

G

A

A

A

T

K

I

I

G

G

N

R

R

G

F

I

F

M

I

L

D

D

H
|
H

G

A

M

T

G

G

V

I

V

V

I

V

G

G

E

E

T

T

A

A

E

V

I

I

G

E

D

D

D

D

C

V

T

S

L

I

Y
x
L

H
x
Q

G

S

V

V

T

T

L

L

G

G

G

G

T

T

T

G

W

K

Q

T

A

S

G

G

K

D

R

R

H

H

P

P

T

K

L

I

G

R

N

E

N

G

V

V

V

M

V

I

G
|
G

A
|
A

G

G

A

A

K

K

I

I

L

L

G

G

P

N

I

I

T

E

M

V

H

G

D

R

G

G

A

A

R

K

V

I

G
|
G

S
x
A

N

G

S

S

V

V

V

V

V

L

K

Q

D

P

V

V

P

P

K

P

D

H

T

T

T
|
T

V

A

V
x
A

G

G

I

V

P

P

G

A

R

R

V

I

V

V

binding coenzyme a: H158 (= H84), L177 (≠ Y103), Q178 (≠ H104), G203 (= G129), A204 (= A130), G221 (= G147), A222 (≠ S148), T235 (= T161), A237 (≠ V163)
binding cysteine: P93 (= P19), H158 (= H84)

4h7oA Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
42% identity, 60% coverage: 10:173/273 of query aligns to 80:243/258 of 4h7oA

query
sites
4h7oA

D

D

I

I

E

C

S

A

I

T

Y

V

H

N

R

R

D
|
D

P
|
P

A

A

A

V

R

S

S

M

A

Y

F

S

E

M

I

P

L

L

V

L

N

Y

Y

L

P

K

G

G

M

Y

H

H

A

A

I

L

W

Q

L

G

H

Y

R

R

I

V

S

A

H

N

K

W

L

L

W

W

K

R

R

Q

D

G

W

R

R

K

L

A

M

L

A

A

R

T

C
x
Y

L

F

S

Q

T

N

F
x
Q

S

I

R

S

W

V

L

A

T

C

G

Q

V

V

E

D

I

I

H

H

P

P

G

A

A

A

T

R

I

I

G

G

N

R

R

G

F

I

F

M

I

L

D
|
D

H
|
H

G

A

M

T

G

G

V

I

V

V

I

I

G

G

E

E

T

T

A

A

E

V

I

V

G

E

D

D

D

D

C

V

T

S

L

I

Y

L

H

Q

G

D

V

V

T

T

L

L

G

G

G
|
G

T

T

T

G

W

K

Q

E

A

C

G

G

K

D

R
|
R

H
|
H

P

P

T

K

L

I

G

R

N

E

N

G

V

V

V

M

V

I

G

G

A

A

G

G

A

A

K

K

I

I

L

L

G

G

P

N

I

I

T

E

M

V

H

G

D

E

G

G

A

A

R

K

V

I

G

G

S

S

N

G

S

S

V

V

V

V

V

L

K

Q

D

A

V

V

P

P

K

P

D

H

T

T

T

T

V

V

V

A

G

G

I

V

P

P

G

A

R

R

V

I

V

V

A

G

T

R

P

P

binding arginine: Y127 (≠ C57), Q131 (≠ F61)
binding cysteine: D88 (= D18), P89 (= P19), D153 (= D83), H154 (= H84), G180 (= G110), R188 (= R118), H189 (= H119)

Sites not aligning to the query:

binding arginine: 51, 53

4n6bA Soybean serine acetyltransferase complexed with coa (see paper)
45% identity, 59% coverage: 10:170/273 of query aligns to 79:233/233 of 4n6bA

query
sites
4n6bA

D

D

I

L

E

R

S

A

I

A

Y

R

H

E

R

R

D

D

P

V

A

-

A

-

R

-

S

S

A

Y

F

S

E

H

I

C

L

L

V

L

N

N

Y

Y

P

K

G

G

M

F

H

L

A

A

I

C

W

Q

L

A

H

H

R

R

I

V

S

A

H

H

K

L

L

L

W

W

K

R

R

Q

D

S

W

R

R

R

L

P

M

L

A

A

R

L

C

A

L

L

S

H

T

S

F

R

S

I

R

A

W

N

L

V

T

F

G

A

V

V

E

D

I

I

H

H

P

P

G

A

A

A

T

R

I

I

G

G

N

K

R

G

F

I

F

L

I

F

D

D

H
|
H

G

A

M

T

G

G

V

V

V

V

I

V

G

G

E

E

T

T

A

A

E

V

I

I

G

G

D

N

D

N

C

V

T

S

L

I

Y
x
L

H

H

G

H

V

V

T

T

L

L

G

G

G
|
G

T

T

T

-

W

-

Q

-

A

G

G

G

K

D

R

R

H

H

P

P

T

K

L

I

G

G

N

D

N

G

V

V

V

L

V

I

G

G

A

A

G

G

A

A

K

T

I

I

L

L

G

G

P

N

I

I

T

K

M

I

H

G

D

E

G

G

A

A

R
x
K

V

V

G
|
G

S
x
A

N

G

S

S

V

V

V

V

V
x
L

K

I

D

D

V

V

P

P

K

P

D

R

T

T

T

T

V

A

V
|
V

G

G

I

N

P

P

G

A

R

R

V

L

V

V

binding coenzyme a: H150 (= H84), L169 (≠ Y103), G176 (= G110), K208 (≠ R145), G210 (= G147), A211 (≠ S148), L216 (≠ V153), V226 (= V163)

3p47A Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-cysteine (see paper)
43% identity, 57% coverage: 7:162/273 of query aligns to 105:268/270 of 3p47A

query
sites
3p47A

L

L

K

K

E

T

D

D

I

L

E

I

S

A

I

A

Y

Y

H

A

R

G

D

D

P

P

A

A

A

A

R

P

S

G

A

L

F

S

E

L

I

I

L

I

V

R

N

C

Y

Y

P

P

G

G

M

F

H

Q

A

A

I

V

W

I

L

V

H

Y

R

R

I

I

S

A

H

H

K

V

L

L

W

Y

K

E

R

C

D

G

W

E

R

R

L

Y

M

Y

A

C

R

R

C

E

L

M

S

M

T

E

F

S

S

V

R

H

W

S

L

Y

T

T

G

S

V

I

E

D

I

I

H

H

P

P

G

G

A

A

T

S

I

I

G

K

N

G

R

H

F

F

F

F

I

I

D

D

H

H

G

G

M

V

G

G

V

V

V

V

I

I

G

G

E

E

T

T

A

A

E

I

I

I

G

G

D

E

D

W

C

C

T

R

L

I

Y

Y

H

Q

G

S

V

V

T

T

L

L

G

G

G
x
A

T

M

T
x
H

W

F

Q

Q

-

E

-

E

-

G

-

G

-

V

-

I

-

K

-

R

A

G

G

T

K

K

R
|
R

H
|
H

P

P

T

T

L

V

G

G

N

D

N

Y

V

V

V

T

V

I

G

G

A

T

G

G

A

A

K

K

I

V

L

L

G

G

P

N

I

I

T

I

M

V

H

G

D

S

G

H

A

V

R

R

V

I

G

G

S

A

N

N

S

C

V

W

V

I

V

D

K

R

D

D

V

V

P

D

K

S

D

N

T

Q

T

T

V

V

binding cysteine: A208 (≠ G110), H210 (≠ T112), R224 (= R118), H225 (= H119)

3q1xA Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-serine (see paper)
43% identity, 57% coverage: 7:162/273 of query aligns to 103:266/267 of 3q1xA

query
sites
3q1xA

L

L

K

K

E

T

D

D

I

L

E

I

S

A

I

A

Y

Y

H

A

R

G

D

D

P

P

A

A

A

A

R

P

S

G

A

L

F

S

E

L

I

I

L

I

V

R

N

C

Y

Y

P

P

G

G

M

F

H

Q

A

A

I

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W

I

L

V

H

Y

R

R

I

I

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A

H

H

K

V

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L

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Y

K

E

R

C

D

G

W

E

R

R

L

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M

Y

A

C

R

R

C

E

L

M

S

M

T

E

F

S

S

V

R

H

W

S

L

Y

T

T

G

S

V

I

E

D

I

I

H

H

P

P

G

G

A

A

T

S

I

I

G

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N

G

R

H

F

F

F

F

I

I

D

D

H

H

G

G

M

V

G

G

V

V

V

V

I

I

G

G

E

E

T

T

A

A

E

I

I

I

G

G

D

E

D

W

C

C

T

R

L

I

Y

Y

H

Q

G

S

V

V

T

T

L

L

G

G

G

A

T

M

T
x
H

W

F

Q

Q

-

E

-

E

-

G

-

G

-

V

-

I

-

K

-

R

A

G

G

T

K

K

R
|
R

H
|
H

P

P

T

T

L

V

G

G

N

D

N

Y

V

V

V

T

V

I

G

G

A

T

G

G

A

A

K

K

I

V

L

L

G

G

P

N

I

I

T

I

M

V

H

G

D

S

G

H

A

V

R

R

V

I

G

G

S

A

N

N

S

C

V

W

V

I

V

D

K

R

D

D

V

V

P

D

K

S

D

N

T

Q

T

T

V

V

binding serine: H208 (≠ T112), R222 (= R118), H223 (= H119)

1sstA Serine acetyltransferase- complex with coa (see paper)
42% identity, 59% coverage: 10:170/273 of query aligns to 80:233/233 of 1sstA

query
sites
1sstA

D

D

I

I

E

Q

S

A

I

V

Y

R

H

H

R

R

D

D

P

P

A

A

A

V

R

E

S

L

A

W

F

S

E

T

I

P

L

L

V

L

N

Y

Y

L

P

K

G

G

M

F

H

H

A

A

I

I

W

Q

L

S

H

Y

R

R

I

I

S

T

H

H

K

Y

L

L

W

W

K

N

R

Q

D

N

W

R

R

K

L

S

M

L

A

A

R

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C

Y

L

L

S

Q

T

N

F

Q

S

I

R

S

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V

L

A

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V

V

E

D

I

I

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H

P

P

G

A

A

A

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K

I

I

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G

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H

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G

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I

F

M

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F

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D

H
|
H

G

A

M

T

G

G

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I

V

V

I

V

G

G

E

E

T

T

A

S

E

V

I

I

G

E

D

N

D

D

C

V

T

S

L

I

Y
x
L

H

Q

G

G

V

V

T

T

L

L

G

G

G
|
G

T
|
T

T

-

W

-

Q

-

A

-

G

-

K

-

R

-

H

H

P

P

T

K

L

V

G

R

N

E

N

G

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V

V

M

V

I

G

G

A
|
A

G

G

A

A

K

K

I

I

L
|
L

G

G

P

N

I

I

T

E

M

V

H

G

D

K

G

Y

A

A

R
x
K

V

I

G
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G

S
x
A

N

N

S

S

V
|
V

V

V

V
x
L

K

N

D

P

V

V

P

P

K

E

D

Y

T

A

T
|
T

V

A

V
x
A

G

G

I
x
V

P
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P

G

A

R

R

V

I

V
|
V

binding coenzyme a: H154 (= H84), L173 (≠ Y103), G180 (= G110), T181 (= T111), A193 (= A130), L198 (= L135), K208 (≠ R145), G210 (= G147), A211 (≠ S148), V214 (= V151), L216 (≠ V153), T224 (= T161), A226 (≠ V163), V228 (≠ I165), P229 (= P166), V233 (= V170)

Q8Z9S7 Bifunctional protein GlmU; EC 2.7.7.23; EC 2.3.1.157 from Yersinia pestis
31% identity, 42% coverage: 49:163/273 of query aligns to 316:438/456 of Q8Z9S7

query
sites
Q8Z9S7

R

E

D

D

W

A

R

R

L

L

M

D

A

A

R

N

C

C

-

T

L

V

S

G

T

P

F

F

S

A

R

R

W

L

L
x
R

T

P

G

G

V

A

E

E

I

L

H

A

P

E

G

G

A

A

T

H

I

V

G

G

N

N

R

F

F

V

F

E

I

I

D
x
K

H

K

-

A

-

R

-

L

G

G

M

K

G

G

V

S

V

K

I

A

G

G

E

H

T

L

A

S

E
x
Y

I

L

G

G

D

D

D

-

C

A

T

E

L

I

Y

G

H

A

G

G

V

V

T
x
N

L

I

G

G

G

A

T

G

T

T

W

I

Q

T

-

C

-

N

-

Y

-

D

-

G

A

A

G

N

K

K

R

F

H

K

P

T

T

I

L

I

G

G

N

D

N

D

V

V

V

F

V

V

G

G

A

S

G

D

A

T

K

Q

I

L

L

V

G

A

P

P

I

V

T

T

M

V

H

A

D

N

G

G

A

A

R

T

V

I

G

G

S

A

N

G

S

T

V

T

V

V

V

T

K

R

D

D

V

V

P

A

K

E

D

N

T

E

T

L

V

V

V

I

R333 (≠ L65) binding
K351 (≠ D83) binding
Y366 (≠ E95) binding
N377 (≠ T107) binding

4fceA Crystal structure of yersinia pestis glmu in complex with alpha-d- glucosamine 1-phosphate (gp1)
31% identity, 42% coverage: 49:163/273 of query aligns to 308:430/441 of 4fceA

query
sites
4fceA

R

E

D

D

W

A

R

R

L

L

M

D

A

A

R

N

C

C

-

T

L

V

S

G

T

P

F

F

S

A

R

R

W

L

L
x
R

T

P

G

G

V

A

E

E

I

L

H

A

P

E

G

G

A

A

T

H

I

V

G

G

N

N

R

F

F

V

F

E

I

I

D
x
K

H

K

-

A

-

R

-

L

G

G

M

K

G

G

V

S

V

K

I

A

G

G

E

H

T

L

A

S

E
x
Y

I

L

G

G

D

D

D

-

C

A

T

E

L

I

Y

G

H

A

G

G

V

V

T

N

L

I

G

G

G

A

T

G

T

T

W

I

Q

T

-

C

-
x
N

-
x
Y

-

D

-

G

A

A

G

N

K
|
K

R

F

H

K

P

T

T

I

L

I

G

G

N

D

N

D

V

V

V

F

V

V

G

G

A

S

G

D

A

T

K

Q

I

L

L

V

G

A

P

P

I

V

T

T

M

V

H

A

D

N

G

G

A

A

R

T

V

I

G

G

S

A

N

G

S

T

V

T

V

V

V

T

K

R

D

D

V

V

P

A

K

E

D

N

T

E

T

L

V

V

V

I

binding 2-amino-2-deoxy-1-O-phosphono-alpha-D-glucopyranose: R325 (≠ L65), K343 (≠ D83), Y358 (≠ E95), N378 (vs. gap), Y379 (vs. gap), K384 (= K117)

Sites not aligning to the query:

active site: 15

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
29% identity, 45% coverage: 76:198/273 of query aligns to 78:199/203 of P07464

query
sites
P07464

I

I

G

G

N

R

R

N

F

F

F

Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V

V

I

D

G
x
D

E

Y

T

T

A

V

E

T

I

I

G

G

D

D

D

N

C

V

T

L

L

I

Y

A

H

P

G

N

V

V

T

T

L

L

G

S

G

V

T

T

-

G

-
x
H

-

P

-

V

-

H

-

H

-

E

T

L

W

R

Q

K

A

N

G

G

K

E

R

M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

G

G

N

N

N

N

V

V

V

W

V

I

G

G

A
x
S

G

H

A

V

K

V

I

I

L

N

G

P

P

G

I

V

T

T

M

I

H

G

D

D

G

N

A

S

R

V

V

I

G

G

S
x
A

N

G

S

S

V

I

V

V

V
x
T

K
|
K

D

D

V

I

P

P

K

P

D

N

T

V

T

V

V

A

V

A

G

G

I

V

P

P

G

C

R
|
R

V

V

V

I

A

-

T

-

P

-

S

-

P

-

Q

-

S

-

K
x
R

E

E

K

I

S

N

E

D

R

R

R

D

S

-

A

-

M

-

A

-

K

K

K

H

Y

Y

G

Y

F

F

D

K

A

D

Y

Y

A

K

V

V

N85 (≠ D83) binding in other chain; binding in other chain
D93 (≠ G91) binding
H115 (vs. gap) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A130) binding in other chain
A160 (≠ S148) binding in other chain
TK 165:166 (≠ VK 153:154) binding
R180 (= R168) binding
R183 (≠ K178) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding
71 binding

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
29% identity, 45% coverage: 76:198/273 of query aligns to 77:198/200 of 1krrA

query
sites
1krrA

I

I

G

G

N

R

R

N

F

F

F

Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V

V

I

D

G

D

E

Y

T

T

A

V

E

T

I

I

G

G

D

D

D

N

C

V

T

L

L
x
I

Y
x
A

H
x
P

G

N

V

V

T

T

L

L

G

S

G

V

T
|
T

-

G

-
x
H

-

P

-

V

-

H

-

H

-

E

T

L

W

R

Q

K

A

N

G

G

K

E

R

M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

G

G

N

N

N

N

V

V

V

W

V

I

G
|
G

A
x
S

G

H

A

V

K

V

I

I

L
x
N

G

P

P

G

I

V

T

T

M

I

H

G

D

D

G

N

A

S

R

V

V

I

G
|
G

S
x
A

N

G

S

S

V

I

V

V

V

T

K

K

D

D

V

I

P

P

K

P

D

N

T

V

T

V

V

A

V
x
A

G

G

I

V

P
|
P

G

C

R

R

V

V

V

I

A

-

T

-

P

-

S

-

P

-

Q

-

S

-

K
x
R

E

E

K

I

S

N

E

D

R

R

R

-

S

-

A

-

M

-

A

D

K

K

K

H

Y

Y

G

Y

F

F

D

K

A

D

Y

Y

A

K

V

V

binding acetyl coenzyme *a: N84 (≠ D83), I103 (≠ L102), A104 (≠ Y103), P105 (≠ H104), T112 (= T111), H114 (vs. gap), G140 (= G129), S141 (≠ A130), N146 (≠ L135), G158 (= G147), A159 (≠ S148), A174 (≠ V163), P177 (= P166), R182 (≠ K178)

Query Sequence

>201412 FitnessBrowser__MR1:201412
MGVIARLKEDIESIYHRDPAARSAFEILVNYPGMHAIWLHRISHKLWKRDWRLMARCLST
FSRWLTGVEIHPGATIGNRFFIDHGMGVVIGETAEIGDDCTLYHGVTLGGTTWQAGKRHP
TLGNNVVVGAGAKILGPITMHDGARVGSNSVVVKDVPKDTTVVGIPGRVVATPSPQSKEK
SERRSAMAKKYGFDAYAVSPDNPDPVANAIGQMLDHMHLMDSKVQELCQAIQRLGGDVCT
ERLPELNVGDFNDVEKAAADKRQSALDEFDPSI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory