SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 201976 FitnessBrowser__MR1:201976 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

8gxkB Pseudomonas jinjuensis n-acetyltransferase (see paper)
32% identity, 93% coverage: 6:186/194 of query aligns to 4:182/188 of 8gxkB

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binding coenzyme a: M40 (≠ T39), T93 (= T97), W94 (= W98), H100 (≠ Q104), G101 (≠ R105), T129 (= T133), N133 (= N137), L134 (≠ Q138), R135 (≠ A139), S136 (= S140), A139 (= A143), K142 (≠ R146)

1yreB Hypothetical protein pa3270 from pseudomonas aeruginosa in complex with coa
38% identity, 69% coverage: 53:186/194 of query aligns to 51:182/187 of 1yreB

query
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binding coenzyme a: T93 (= T97), W94 (= W98), H100 (≠ Q104), G101 (≠ R105), N105 (= N109), R135 (≠ A139), G138 (≠ Q142)

Sites not aligning to the query:

binding coenzyme a: 40

8gxfB Pseudomonas flexibilis gcn5 family acetyltransferase (see paper)
31% identity, 89% coverage: 15:186/194 of query aligns to 13:182/187 of 8gxfB

query
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binding coenzyme a: T93 (= T97), H100 (≠ Q104), G101 (≠ R105), K109 (= K113), T129 (= T133), R135 (≠ A139)

Query Sequence

>201976 FitnessBrowser__MR1:201976
MGNDDLSACLNGEYIVLEPLSLSHVAALTDGELWRLWFTSVPEPSEMKAYVMKALAGKSR
GECFPFAVRDKVSGEIVGCTRICHWESEHRHLEIGYTWYAKRAQRTGINTDAKLLLLTFA
FETLEAIAVEFRTHWHNQASRQAIARLGAKQDRVLRNNKILKDGTIRDTVVYSIIDSEWV
TVKQHLLFRLQQYP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory