SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 202167 SO3048 isoquinoline 1-oxidoreductase, beta subunit, putative (NCBI ptt file) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8gy3C Cryo-em structure of membrane-bound aldehyde dehydrogenase from gluconobacter oxydans
28% identity, 94% coverage: 37:742/748 of query aligns to 6:718/732 of 8gy3C

query
sites
8gy3C

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binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): M38 (= M69), G39 (= G70), Q40 (= Q71), H41 (≠ G72), V42 (≠ I73), A45 (≠ G76), G79 (≠ D109), G80 (= G110), S81 (= S111), S83 (= S113), V84 (≠ I114), G374 (= G402), F375 (= F403), L379 (≠ S407), L499 (≠ M517), R500 (= R518), V624 (= V648), D625 (≠ N649), Q632 (= Q656), T687 (≠ A711), G688 (= G712), L689 (≠ V713), G690 (= G714), E691 (= E715)

Q0QLF2 Nicotinate dehydrogenase large molybdopterin subunit; NDH; Nicotinic acid hydroxylase large molybdopterin subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see 2 papers)
25% identity, 35% coverage: 189:448/748 of query aligns to 3:285/425 of Q0QLF2

query
sites
Q0QLF2

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C

Q208 (= Q372) binding
GFG 238:240 (= GFG 402:404) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2:425 modified: mature protein, Nicotinate dehydrogenase large molybdopterin subunit

3hrdE Crystal structure of nicotinate dehydrogenase (see paper)
25% identity, 35% coverage: 189:448/748 of query aligns to 2:284/420 of 3hrdE

query
sites
3hrdE

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V

A

A

T

A

N

Q

T

T

W

P

S

D

A

L

L

V

Q

Q

-

E

G

A

R

L

K

D

A

A

L

I

K

T

V

I

T

E

W

Y

T

-

Q

E

S

E

A

L

N

E

S

G

S

I

H

F

D

T

S

M

K

E

T

R

Y

A

L

L

Q

E

E

E

L

D

V

S

D

P

K

A

V

I

Q

H

A

G

P

D

G

T

K

N

V

I

V

H

R

Q

-

V

-

K

-

H

-

L

Q

E

V

Y

G

G

D

D

E

V

V

D

K

A

A

A

W

F

P

K

E

Q

A

C

N

D

-

I

T

V

L

V

K

E

A

D

I

T

Y

Y

T

S

V

T

P

H

Y

R

L

L

I

T

H

H

S

M

S

F

I

I

E

E

P

P

P

D

V

A

A

G

T

V

A

S

N

Y

V

Y

T

D

E

N

K

E

G

G

-

M

C

L

E

T

I

V

W

V

A

V

S

S

T
|
T

Q
|
Q

T
x
N

P

P

Q

H

S

Y

T
x
D

Q

R

Q

G

N

E

V

V

A

A

A

G

A

M

L

L

G

A

I

L

A

P

E

N

D

S

A

K

V

V

K

R

V

I

N

I

V

Q

T

A

L

T

G

G

G
|
G

F
|
F

G

G

R

G

K
|
K

S
x
L

K
x
D

P

L

D

S

F

V

S

Q

V

C

E

H

A

C

A

A

L

L

S

T

K

Y

Q

H

L

T

K

K

R

K

P

P

V

V

K

K

V

M

S

V

W

R

S

S

R

R

E

E

D

E

E

S

I

T

Q

T

-

V

N

S

G

S

Y

K

Y

R

H

H

A

P

I

M

S

T

A

M

Q

H

C

C

active site: Q207 (= Q372), L242 (≠ S407)
binding calcium ion: T206 (= T371), N208 (≠ T373), D212 (≠ T377), K241 (= K406), L242 (≠ S407), D243 (≠ K408)
binding pterin cytosine dinucleotide: G237 (= G402), F238 (= F403)
binding selenium atom: F238 (= F403)

Sites not aligning to the query:

active site: 318, 322, 350
binding pterin cytosine dinucleotide: 350
binding selenium atom: 348, 349, 350

3hrdA Crystal structure of nicotinate dehydrogenase (see paper)
25% identity, 35% coverage: 189:448/748 of query aligns to 2:284/420 of 3hrdA

query
sites
3hrdA

K

K

D

D

F

Y

K

Q

Q

V

I

L

G

G

A

K

S

N

R

K

Q

V

I

K

V

V

D

D

M

S

Q

L

A

E

M

K

L

V

T

M

G

G

K

T

A

A

V

K

Y

F

G

A

Y

A

D

D

I

Y

Q

S

V

F

D

P

N

D

M

M

L

L

Y

Y

A

A

S

G

I

V

V

F

R

R

P

S

P

T

V

V

L

P

G

H

S

A

D

R

V

I

A

V

S

S

L

L

D

D

D

L

T

S

A

K

A

A

R

R

K

A

V

I

Q

D

G

G

V

V

-

E

-

A

-

V

V

L

D

D

V

Y

Y

H

R

A

L

I

P

P

T

G

P

K

-

N

-

R

-

F

-

G

-

I

K

K

G

D

A

E

P

P

A

C

F

L

-

V

-

D

-

K

-

V

-

R

Q

R

A

Y

L

G

G

D

G

A

V

I

A

A

V

V

A

A

T

A

N

Q

T

T

W

P

S

D

A

L

L

V

Q

Q

-

E

G

A

R

L

K

D

A

A

L

I

K

T

V

I

T

E

W

Y

T

-

Q

E

S

E

A

L

N

E

S

G

S

I

H

F

D

T

S

M

K

E

T

R

Y

A

L

L

Q

E

E

E

L

D

V

S

D

P

K

A

V

I

Q

H

A

G

P

D

G

T

K

N

V

I

V

H

R

Q

-

V

-

K

-

H

-

L

Q

E

V

Y

G

G

D

D

E

V

V

D

K

A

A

A

W

F

P

K

E

Q

A

C

N

D

-

I

T

V

L

V

K

E

A

D

I

T

Y

Y

T

S

V

T

P

H

Y

R

L

L

I

T

H

H

S

M

S

F

I

I

E

E

P

P

P

D

V

A

A

G

T

V

A

S

N

Y

V

Y

T

D

E

N

K

E

G

G

-

M

C

L

E

T

I

V

W

V

A

V

S

S

T
|
T

Q
|
Q

T
x
N

P

P

Q

H

S

Y

T
x
D

Q

R

Q

G

N

E

V

V

A

A

A

G

A

M

L

L

G

A

I

L

A

P

E

N

D

S

A

K

V

V

K

R

V

I

N

I

V

Q

T

A

L

T

G

G

G
|
G

F
|
F

G
|
G

R

G

K
|
K

S
x
L

K
x
D

P

L

D

S

F

V

S

Q

V

C

E

H

A

C

A

A

L

L

S

T

K

Y

Q

H

L

T

K

K

R

K

P

P

V

V

K

K

V

M

S

V

W

R

S

S

R

R

E

E

D

E

E

S

I

T

Q

T

-

V

N

S

G

S

Y

K

Y

R

H

H

A

P

I

M

S

T

A

M

Q

H

C

C

active site: Q207 (= Q372), L242 (≠ S407)
binding pterin cytosine dinucleotide: G236 (= G401), G237 (= G402), F238 (= F403)
binding magnesium ion: T206 (= T371), N208 (≠ T373), D212 (≠ T377), K241 (= K406), L242 (≠ S407), D243 (≠ K408)
binding selenium atom: F238 (= F403), G239 (= G404)

Sites not aligning to the query:

active site: 318, 322, 350
binding pterin cytosine dinucleotide: 350
binding magnesium ion: 305, 308, 309, 346
binding nicotinic acid: 314, 318, 352
binding selenium atom: 348, 349, 350

5y6qC Crystal structure of an aldehyde oxidase from methylobacillus sp. Ky4400 (see paper)
35% identity, 16% coverage: 623:743/748 of query aligns to 614:743/748 of 5y6qC

query
sites
5y6qC

M

V

R

E

V

V

E

R

V

V

S

D

E

E

D

D

-

F

K

G

Q

T

L

I

K

R

V

V

L

K

N

R

A

M

I

V

A

A

I

L

D

D

A

S

G

G

T
x
R

V

L

V
x
Y

N
|
N

P

P

D

K

R

L

V

A

K

R

A

S

Q
|
Q

T

W

E

I

G

G

A

G

I

M

V

I

F

M

G

G

L

V

S

G

L

Q

A

A

L

L

M

M

G

E

E

E

-

G

-

I

I

V

S

D

Y

P

Q

R

E

N

G

G

K

R

V

V

V

I

Q

N

S

N

N

N

F

L

H

A

D

D

Y

Y

P

L

L

V

L

P

R

V

L

N

P

G

Q

D

V

I

P

P

E

S

I

I

E

T

I

T

I

I

I

N

I

V

-

G

-

E

-

P

D

D

S

F

D

E

A

A

P

T

A

M

G

G

-

G

-
x
K

-

A

V
|
V

G
|
G

E
|
E

P
x
L

G

G

V

I

P

V

P

G

V

V

A

A

P

A

S

A

L

I

T

S

N

N

A

A

I

V

F

F

A

H

A

A

T

T

G

G

V

K

R

R

I

V

R

R

D

G

L

L

P

P

V

I

N

T

active site: E715 (= E715), L716 (≠ P716)
binding pterin cytosine dinucleotide: R638 (≠ T646), Y640 (≠ V648), N641 (= N649), Q648 (= Q656), K711 (vs. gap), V713 (= V713), G714 (= G714), E715 (= E715)

Sites not aligning to the query:

active site: 204, 239, 310, 316, 344
binding pterin cytosine dinucleotide: 233, 234, 235, 461, 462, 463, 464, 468, 500, 502, 503, 504, 505

P77489 Aldehyde oxidoreductase molybdenum-binding subunit PaoC; EC 1.2.99.6 from Escherichia coli (strain K12) (see 2 papers)
31% identity, 15% coverage: 632:742/748 of query aligns to 601:719/732 of P77489

query
sites
P77489

Q

E

L

V

K

R

V

V

L

R

N

R

A

M

I

L

A

A

A

V

I

C

D

A

A

A

G

G

T
x
R

V
x
I

V
x
L

N
|
N

P
|
P

D
x
K

R
x
T

V

A

K

R

A

S

Q
|
Q

T

V

E

I

G

G

A

A

I

M

V

T

F

M

G

G

L

M

S

G

L

A

A

A

L

L

M

M

G

E

E

E

I

L

S

A

Y

V

Q

D

E

D

-

R

-

L

G

G

K

Y

V

F

V

V

Q

N

S

H

N

D

F

M

H

A

D

G

Y

Y

P

E

L

V

L

P

R

V

L

H

P

A

Q

D

V

I

P

P

E

K

I

Q

E

E

I

V

I

I

I

F

I

L

D

D

S

D

D

T

A

D

P

P

P

I

A

S

-

S

-

P

-

M

-

K

-

A

-
x
K

G
|
G

V
|
V

G
|
G

E
|
E

P

L

G

G

V

L

P

C

P

G

V

V

A

S

P

A

S

A

L

I

T

A

N

N

A

A

I

V

F

Y

A

N

A

A

T

T

G

G

V

I

R

R

I

V

R

R

D

D

L

Y

P

P

V

I

615:621 (vs. 646:652, 29% identical) binding
Q625 (= Q656) binding
KGVG 688:691 (≠ -GVG 712:714) binding
E692 (= E715) mutation to Q: Loss of activity.

Sites not aligning to the query:

241:242 binding
440 mutation R->H,K: Decrease in catalytic efficiency.
468:470 binding
511:512 binding

5g5gC Escherichia coli periplasmic aldehyde oxidase (see paper)
31% identity, 15% coverage: 632:743/748 of query aligns to 601:720/731 of 5g5gC

query
sites
5g5gC

Q

E

L

V

K

R

V

V

L

R

N

R

A

M

I

L

A

A

A

V

I

C

D

A

A

A

G

G

T

R

V

I

V
x
L

N
|
N

P

P

D

K

R
x
T

V

A

K

R

A

S

Q
|
Q

T

V

E

I

G

G

A

A

I

M

V

T

F

M

G

G

L

M

S

G

L

A

A

A

L

L

M

M

G

E

E

E

I

L

S

A

Y

V

Q

D

E

D

-

R

-

L

G

G

K

Y

V

F

V

V

Q

N

S

H

N

D

F

M

H

A

D

G

Y

Y

P

E

L

V

L

P

R

V

L

H

P

A

Q

D

V

I

P

P

E

K

I

Q

E

E

I

V

I

I

I

F

I

L

D

D

S

D

D

T

A

D

P

P

P

I

A

S

-

S

-

P

-

M

-

K

-

A

-
x
K

G

G

V
|
V

G
|
G

E
|
E

P
x
L

G

G

V

L

P

C

P

G

V

V

A

S

P

A

S

A

L

I

T

A

N

N

A

A

I

V

F

Y

A

N

A

A

T

T

G

G

V

I

R

R

I

V

R

R

D

D

L

Y

P

P

V

I

N

T

active site: E692 (= E715), L693 (≠ P716)
binding pterin cytosine dinucleotide: L617 (≠ V648), N618 (= N649), T621 (≠ R652), Q625 (= Q656), K688 (vs. gap), V690 (= V713), G691 (= G714), E692 (= E715)

Sites not aligning to the query:

active site: 211, 246, 316, 322, 350
binding pterin cytosine dinucleotide: 240, 241, 242, 350, 468, 469, 470, 507, 509, 511, 512

1rm6A Structure of 4-hydroxybenzoyl-coa reductase from thauera aromatica (see paper)
35% identity, 14% coverage: 641:743/748 of query aligns to 639:746/761 of 1rm6A

query
sites
1rm6A

A

A

I

V

D

D

A

V

G

G

T

K

V

A

V
x
L

N
|
N

P

P

D

L

R

A

V
|
V

K

E

A

G

Q
|
Q

T

T

E

Q

G

G

A

G

I

V

V

W

F

M

G

G

L

M

S

G

L

Q

A

A

L

L

M

S

G

E

E

E

I

T

S

V

Y

Y

Q

D

E

N

G

G

K

R

V

M

V

V

Q

H

S

G

N

N

F

I

H

L

D

D

Y

Y

P

R

L

V

L

P

R

T

L

I

P

V

Q

E

V

S

P

P

E

D

I

I

E

E

I

V

I

I

I

V

D

E

S

S

D

M

A

D

P

P

-

N

-

G

P

P

A

F

G

G

V

A

G
x
K

E
|
E

P
x
A

G
x
S

V
x
E

P
x
G

P

M

V

L

A

A

-

G

-

F

-

L

P

P

S

A

L

I

T

H

N

E

A

A

I

V

F

Y

A

E

A

A

T

V

G

G

V

V

R

R

I

A

R

T

D

D

L

F

P

P

V

L

N

S

active site: E718 (≠ V718), G719 (≠ P719)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): L646 (≠ V648), N647 (= N649), V651 (= V653), Q654 (= Q656), K714 (≠ G714), E715 (= E715), A716 (≠ P716), S717 (≠ G717), E718 (≠ V718)

Sites not aligning to the query:

active site: 206, 241, 318, 322, 350
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 235, 236, 237, 238, 350, 473, 474, 475, 476, 513, 514, 515, 517, 518

O33819 4-hydroxybenzoyl-CoA reductase subunit alpha; 4-HBCR subunit alpha; EC 1.1.7.1 from Thauera aromatica (see paper)
35% identity, 14% coverage: 641:743/748 of query aligns to 647:754/769 of O33819

query
sites
O33819

A

A

I

V

D

D

A
x
V

G
|
G

T
x
K

V
x
A

V
x
L

N
|
N

P

P

D

L

R

A

V

V

K

E

A

G

Q

Q

T

T

E

Q

G

G

A

G

I

V

V

W

F

M

G

G

L

M

S

G

L

Q

A

A

L

L

M

S

G

E

E

E

I

T

S

V

Y

Y

Q

D

E

N

G

G

K

R

V

M

V

V

Q

H

S

G

N

N

F

I

H

L

D

D

Y

Y

P

R

L

V

L

P

R

T

L

I

P

V

Q

E

V

S

P

P

E

D

I

I

E

E

I

V

I

I

I

V

D

E

S

S

D

M

A

D

P

P

-

N

-

G

P

P

A

F

G

G

V

A

G
x
K

E
|
E

P
x
A

G
x
S

V

E

P

G

P

M

V

L

A

A

-

G

-

F

-

L

P

P

S

A

L

I

T

H

N

E

A

A

I

V

F

Y

A

E

A

A

T

V

G

G

V

V

R

R

I

A

R

T

D

D

L

F

P

P

V

L

N

S

VGKALN 650:655 (≠ AGTVVN 644:649) binding
KEAS 722:725 (≠ GEPG 714:717) binding

Sites not aligning to the query:

214 binding
244:245 binding
522:526 binding

3hrdB Crystal structure of nicotinate dehydrogenase (see paper)
32% identity, 18% coverage: 605:742/748 of query aligns to 175:316/330 of 3hrdB

query
sites
3hrdB

Q

S

G

G

W

I

G

P

F

F

A

E

V

V

H

Y

R

-

S

S

F

Y

V

A

S

T

V

T

V

I

A

A

V

-

A

-

M

-

R

E

V

V

E

E

V

V

-

D

S

T

E

E

D

T

K

G

Q

E

L

V

K

D

V

V

L

L

N

K

A

V

I

V

A

S

A

A

I

H

D

D

A

V

G

G

T

T

V

P

V
x
I

N
|
N

P

R

D

S

R
x
M

V
|
V

K

E

A

G

Q
|
Q

T

I

E

E

G

G

A

G

I

V

V

T

F

M

G

G

L

Q

S

G

L

F

A

V

L

L

M

M

G

E

E

E

I

I

-

E

-

V

S

N

Y

T

Q

K

E

N

G

G

K

A

V

I

V

K

Q

N

S

P

N

S

F

M

H

S

D

K

Y

Y

P

I

L

I

L

P

R

S

L

N

P

R

Q

D

V

V

P

P

E

E

I

I

E

H

I

S

I

I

I

L

I

V

D

E

S

S

D

E

A

G

P

G

P

P

A

G

-

P

-

F

-

G

-

A

-
x
K

G
|
G

V
|
V

G
|
G

E
|
E

P
|
P

G

A

V

L

P

I

P

P

V

M

A

I

P

P

S

A

L

V

T

V

N

A

A

A

I

I

F

E

A

D

A

A

T

L

G

G

V

T

R

R

I

F

R

T

D

H

L

T

P

P

V

I

active site: E289 (= E715), P290 (= P716)
binding pterin cytosine dinucleotide: I215 (≠ V648), N216 (= N649), M219 (≠ R652), V220 (= V653), Q223 (= Q656), K285 (vs. gap), G286 (= G712), V287 (= V713), G288 (= G714), E289 (= E715)

Sites not aligning to the query:

binding pterin cytosine dinucleotide: 45, 46, 47, 48, 49, 85, 87, 89, 90
binding nicotinic acid: 17, 18

Q0QLF1 Nicotinate dehydrogenase medium molybdopterin subunit; NDH; Nicotinic acid hydroxylase medium molybdopterin subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see paper)
32% identity, 18% coverage: 605:742/748 of query aligns to 175:316/330 of Q0QLF1

query
sites
Q0QLF1

Q

S

G

G

W

I

G

P

F

F

A

E

V

V

H

Y

R

-

S

S

F

Y

V

A

S

T

V

T

V

I

A

A

V

-

A

-

M

-

R

E

V

V

E

E

V

V

-

D

S

T

E

E

D

T

K

G

Q

E

L

V

K

D

V

V

L

L

N

K

A

V

I

V

A

S

A

A

I

H

D

D

A
x
V

G
|
G

T
|
T

V
x
P

V
x
I

N
|
N

P
x
R

D
x
S

R
x
M

V
|
V

K
x
E

A
x
G

Q
|
Q

T

I

E

E

G

G

A

G

I

V

V

T

F

M

G

G

L

Q

S

G

L

F

A

V

L

L

M

M

G

E

E

E

I

I

-

E

-

V

S

N

Y

T

Q

K

E

N

G

G

K

A

V

I

V

K

Q

N

S

P

N

S

F

M

H

S

D

K

Y

Y

P

I

L

I

L

P

R

S

L

N

P

R

Q

D

V

V

P

P

E

E

I

I

E

H

I

S

I

I

I

L

I

V

D

E

S

S

D

E

A

G

P

G

P

P

A

G

-

P

-

F

-

G

-
x
A

-
x
K

G
|
G

V
|
V

G
|
G

E
|
E

P

P

G

A

V

L

P

I

P

P

V

M

A

I

P

P

S

A

L

V

T

V

N

A

A

A

I

I

F

E

A

D

A

A

T

L

G

G

V

T

R

R

I

F

R

T

D

H

L

T

P

P

V

I

211:223 (vs. 644:656, 38% identical) binding
AKGVGE 284:289 (≠ --GVGE 712:715) binding

Sites not aligning to the query:

45:49 binding
85:90 binding

4zohA Crystal structure of glyceraldehyde oxidoreductase (see paper)
32% identity, 16% coverage: 625:742/748 of query aligns to 571:695/701 of 4zohA

query
sites
4zohA

V

V

E

K

V

V

S

D

E

G

D

T

K

G

Q

K

L

V

K

F

V

V

L

K

N

K

A

Y

I

V

A

A

A

V

I

D

D

D

A

V

G

G

T

T

V

V

V
x
I

N
|
N

P

P

D

L

R
x
L

V

A

K

E

A

G

Q
|
Q

T

A

E

I

G

G

A

G

I

I

V

V

F

Q

G

G

L

M

S

A

L

Q

A

A

L

L

M

L

-

E

G

G

E

A

I

F

S

F

Y

D

Q

E

E

N

G

G

K

Q

V

L

Q

T

S

T

N

N

F

F

H

Q

D

D

Y

Y

P

P

L

I

L

P

R

T

L

A

P

V

Q

E

V

I

P

P

E

E

-

K

-

I

-

D

-

W

I

Y

E

Y

I

E

I

I

I

L

I

G

D

K

S

S

D

P

A

H

P

P

P

T

A

G

-

S

-
x
K

G
|
G

V
x
I

G
|
G

E
|
E

P
x
A

G

G

V

A

P

I

P

A

V

A

A

T

P

P

S

T

L

I

T

I

N

N

A

A

I

V

F

E

A

Q

A

C

T

I

G

K

V

K

R

R

I

I

R

T

D

K

L

M

P

P

V

V

active site: E668 (= E715), A669 (≠ P716)
binding pterin cytosine dinucleotide: I594 (≠ V648), N595 (= N649), L598 (≠ R652), Q602 (= Q656), K664 (vs. gap), G665 (= G712), I666 (≠ V713), G667 (= G714), E668 (= E715)

Sites not aligning to the query:

active site: 186, 219, 298, 300, 304, 332
binding pterin cytosine dinucleotide: 213, 214, 215, 332, 442, 443, 444, 446, 482, 484, 486, 487

Q7G9P4 Abscisic-aldehyde oxidase; Aldehyde oxidase 3; AO-3; AtAO-3; AtAO4; Indole-3-acetaldehyde oxidase; IAA oxidase; EC 1.2.3.14; EC 1.2.3.7 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
33% identity, 19% coverage: 324:462/748 of query aligns to 718:861/1332 of Q7G9P4

query
sites
Q7G9P4

V

V

G

G

D

D

E

V

V

I

K

K

A

G

W

M

P

E

E

E

A

A

N

E

T

-

L

-

K

R

A

K

I

I

Y

I

T

S

V

S

P

E

Y

L

L

R

I

L

H

G

S

S

S

Q

-

Y

-

F

-

Y

I

M

E

E

P

P

P

Q

V

T

A

A

T

L

A

A

N

L

V

P

T

D

E

E

K

D

G

N

C

C

-

V

E

K

I

V

W

F

A

S

S

S

T

S

Q

Q

T

A

P

P

Q

E

S

Y

T

V

Q

H

Q

S

N

V

V

I

A

A

A

T

A

C

L

L

G

G

I

I

A

Q

E

E

D

H

A

N

V

V

K

R

V

V

N

I

V

T

T

R

L

R

L

V

G

G

F

F

G

G

R

G

K

K

S

A

K

V

P

K

D

S

F

M

S

P

V

V

-

A

-

T

E

A

A

C

A

A

L

L

L

G

S

A

K

Y

Q

K

L
|
L

K
x
Q

R
|
R

P
|
P

V
|
V

K
|
K

V

M

S

F

W

L

S

N

R

R

E

K

-

T

D

D

E

M

I

I

Q

M

N

A

G

G

Y

G

Y

R

H

H

A

P

I

M

S

K

A

I

Q

N

C

-

Y

Y

Q

N

A

V

L

G

F

F

D

R

T

S

N

D

N

G

Q

K

P

L

T

T

A

A

L

L

LQRPVK 821:826 (≠ LKRPVK 422:427) mutation to WDLDQ: In aao3-3; wilty phenotype in rosette leaves, reduced ABA levels, reduced dormancy, abnormal water loss and abnormal response to water deficit.

1t3qB Crystal structure of quinoline 2-oxidoreductase from pseudomonas putida 86 (see paper)
29% identity, 33% coverage: 367:611/748 of query aligns to 219:462/786 of 1t3qB

query
sites
1t3qB

I

L

W

W

A

T

S

A

T

T

Q
|
Q

T

M

P

P

Q

S

S

F

T

V

Q

R

Q

T

N

M

V

V

A

A

A

M

A

F

L

C

G

A

I

I

A

P

E

E

D

H

A

L

V

I

K

E

V

V

N

R

V

V

T

P

L

D

L

V

G

G

G
|
G

F
|
F

G

G

R

Q

K

K

S
x
A

-

H

-

L

K

H

P

P

D

E

F

-

S

E

V

L

E

L

A

V

A

C

L

L

S

S

K

R

Q

A

L

L

K

G

R

R

P

P

V

V

K

R

V

-

S

-

W

W

S

I

R

-

E

E

D

D

E

R

I

Q

Q

E

N

N

-

F

-

L

G

G

Y

A

Y

T

H

H

A

A

I

K

S

Q

A

Q

Q

R

C

N

Y

E

Q

M

A

G

L

L

-

A

F

F

D

D

T

G

N

D

N

G

Q

R

P

F

T

L

A

A

L

L

L

E

A

N

R

R

T

S

-

I

-

T

-

D

G

G

F

G

P

A

S

Y

I

N

S

N

S

L

T

P

F

W

A

T

E

Q

G

L

V

V

E
|
E

Y

S

P

H

S

V

D

G

G

N

E

A

L
x
V

D

I

L

L

G

G

F

V

V

-

D

-

V

-

P

-

F

Y

A

K

L

V

P

P

H

A

L

V

R

S

Y

E

E

E

A

S

V

I

K

A

-

V

A

A

T

T

A

N

H

K

S

C

R

P

I

I

G

G

W

A

M

Y

R
|
R

S

G

V

V

C

-

N

-

I

-

Q

-

H

-

G

G

F

F

G

T

V

A

G

G

-

Q

-

I

-

A

-

R

-

E

S

T

F

L

V

I

D

D

E

R

M

A

A

A

H

R

K

Q

A

L

Q

G

L

L

S

S

C

P

P

F

D

E

M

I

W

R

R

R

S

R

L

N

L

V

G

V

Q

M

P

P

R

E

R

D

E

F

T

P

F

F

D

T

N

N

Q

-

G

-

F

-

K

R

Y

L

G

G

N

Q

Y

T

G

H

E

R

E

E

L

-

T

-

R

-

H

-

P

-

V

-

D

-

I

-

G

G

R

T

Y

Y

L

L

K

Q

V

T

I

I

-

N

-

L

-

E

-

M

-

V

-

N

-

P

E

E

A

A

V

F

E

R

Q

Q

A

R

Q

Q

A

A

K

E

A

A

P

R

K

A

A

R

G

G

K

K

N

Y

Q

L

G

G

W

L

G

G

F

V

A

S

V

V

active site: Q224 (= Q372), A259 (≠ S407), E336 (= E480), V343 (≠ L487), R371 (= R518)
binding pterin cytosine dinucleotide: G254 (= G402), F255 (= F403), R371 (= R518)

Sites not aligning to the query:

active site: 743, 744
binding pterin cytosine dinucleotide: 506, 507, 508, 510, 513, 545, 547, 549, 550, 666, 670, 674, 675, 678, 739, 740, 741, 742

2e3tA Crystal structure of rat xanthine oxidoreductase mutant (w335a and f336l) (see paper)
26% identity, 33% coverage: 202:447/748 of query aligns to 556:818/1291 of 2e3tA

query
sites
2e3tA

D

N

M

M

Q

Q

A

A

M

-

L

-

T

S

G

G

K

E

A

A

V

V

Y

Y

G

C

Y

D

D

D

I

I

-

P

Q

R

V

Y

D

E

N

N

M

E

L

L

Y

S

A

L

S

R

I

L

V

V

R

T

P

S

P

T

V

R

L

A

G

H

S

A

D

K

V

I

A

T

S

S

L

I

D

D

D

T

T

S

A

E

A

A

R

K

K

K

V

V

Q

P

G

G

V

F

V

V

D

C

V

F

Y

L

R

T

-

A

-

E

-

D

L

V

P

P

T

N

P

S

K

N

G

A

A

T

P

G

A

L

F

F

Q

N

-

D

-

E

-

T

-

V

-

F

-

A

-

K

-

D

-

E

-

V

-

T

A

C

L

V

G

G

G

H

V

I

-

I

-

G

A

A

V

V

A

A

T

D

N

T

T

P

W

E

S

H

A

A

L

Q

Q

R

G

A

R

A

K

R

A

G

L

V

K

K

V

I

T

T

W

Y

T

-

Q

-

S

-

A

-

N

-

S

E

S

D

H

L

D

P

S

A

K

I

T

I

Y

T

L

I

Q

Q

E

D

L

A

V

I

D

N

K

N

V

N

Q

S

A

F

P

Y

G

G

K

S

V

E

V

I

R

K

-

I

Q

E

V

K

G

G

D

D

E

L

V

K

K

K

A

G

W

F

P

S

E

E

A

A

-

D

N

N

T

V

L

V

K

S

A

G

I

E

Y

L

T

Y

V

I

P

G

Y

G

L

Q

I
x
E

H
|
H

S

F

S
x
Y

I
x
L

E

E

P

T

P

N

V

C

A

T

T

I

A

A

N

-

V

-

T

V

E

P

K

K

G

G

-

E

-

A

-

G

-

E

C

M

E

E

I

L

W

F

A

V

S

S

T

T

Q
|
Q

T

N

P

T

Q

M

S

K

T

T

Q

Q

Q

S

N

F

V

V

A

A

A

K

A

M

L

L

G

G

I

V

A

P

E

D

D

N

A

R

V

I

K

V

V

V

N

R

V

V

T

K

L

R

L

M

G

G

F

F

G

G

R

G

K

K

S
x
E

K

T

P

R

D

S

-

T

-

V

F

V

S

S

V

T

E

A

A

L

A

A

L

L

L

A

S

A

K

H

Q

K

L

T

K

G

R

R

P

P

V

V

K

R

V

C

S

M

W

L

S

D

R

R

-

D

E

E

D

D

E

M

I

L

Q

I

N
x
T

G
|
G

Y

G

Y
x
R

H
|
H

A

P

I

F

S

L

A

A

Q

K

active site: Q740 (= Q372), E775 (≠ S407)
binding bicarbonate ion: R812 (≠ Y441), H813 (= H442)
binding calcium ion: E713 (≠ I347), H714 (= H348), Y716 (≠ S350), T809 (≠ N438), G810 (= G439)
binding fe2/s2 (inorganic) cluster: L717 (≠ I351)
binding uric acid: E775 (≠ S407)

Sites not aligning to the query:

active site: 853, 857, 885, 1233, 1234
binding bicarbonate ion: 850, 882, 883, 887, 888, 891
binding calcium ion: 840, 843, 844, 847, 880, 881
binding flavin-adenine dinucleotide: 44, 228, 229, 230, 231, 232, 233, 234, 235, 236, 309, 318, 319, 322, 323, 325, 326, 331, 332, 375, 376
binding fe2/s2 (inorganic) cluster: 40, 41, 42, 44, 46, 47, 49, 69, 71, 109, 110, 113, 145, 147
binding uric acid: 853, 887, 982, 983, 1051, 1052, 1234

6a7xB Rat xanthine oxidoreductase, d428a variant, NAD bound form
26% identity, 33% coverage: 202:447/748 of query aligns to 554:816/1291 of 6a7xB

query
sites
6a7xB

D

N

M

M

Q

Q

A

A

M

-

L

-

T

S

G

G

K

E

A

A

V

V

Y

Y

G

C

Y

D

D

D

I

I

-

P

Q

R

V

Y

D

E

N

N

M

E

L

L

Y

S

A

L

S

R

I

L

V

V

R

T

P

S

P

T

V

R

L

A

G

H

S

A

D

K

V

I

A

T

S

S

L

I

D

D

D

T

T

S

A

E

A

A

R

K

K

K

V

V

Q

P

G

G

V

F

V

V

D

C

V

F

Y

L

R

T

-

A

-

E

-

D

L

V

P

P

T

N

P

S

K

N

G

A

A

T

P

G

A

L

F

F

Q

N

-

D

-

E

-

T

-

V

-

F

-

A

-

K

-

D

-

E

-

V

-

T

A

C

L

V

G

G

G

H

V

I

-

I

-

G

A

A

V

V

A

A

T

D

N

T

T

P

W

E

S

H

A

A

L

Q

Q

R

G

A

R

A

K

R

A

G

L

V

K

K

V

I

T

T

W

Y

T

-

Q

-

S

-

A

-

N

-

S

E

S

D

H

L

D

P

S

A

K

I

T

I

Y

T

L

I

Q

Q

E

D

L

A

V

I

D

N

K

N

V

N

Q

S

A

F

P

Y

G

G

K

S

V

E

V

I

R

K

-

I

Q

E

V

K

G

G

D

D

E

L

V

K

K

K

A

G

W

F

P

S

E

E

A

A

-

D

N

N

T

V

L

V

K

S

A

G

I

E

Y

L

T

Y

V

I

P

G

Y

G

L

Q

I

E

H

H

S

F

S

Y

I
x
L

E

E

P

T

P

N

V

C

A

T

T

I

A

A

N

-

V

-

T

V

E

P

K

K

G

G

-

E

-

A

-

G

-

E

C

M

E

E

I

L

W

F

A

V

S

S

T

T

Q
|
Q

T

N

P

T

Q

M

S

K

T

T

Q

Q

Q

S

N

F

V

V

A

A

A

K

A

M

L

L

G

G

I

V

A

P

E

D

D

N

A

R

V

I

K

V

V

V

N

R

V

V

T

K

L

R

L

M

G

G

F

F

G

G

R

G

K

K

S
x
E

K

T

P

R

D

S

-

T

-

V

F

V

S

S

V

T

E

A

A

L

A

A

L

L

L

A

S

A

K

H

Q

K

L

T

K

G

R

R

P

P

V

V

K

R

V

C

S

M

W

L

S

D

R

R

-

D

E

E

D

D

E

M

I

L

Q

I

N

T

G

G

Y

G

Y
x
R

H
|
H

A

P

I

F

S

L

A

A

Q

K

active site: Q738 (= Q372), E773 (≠ S407)
binding bicarbonate ion: R810 (≠ Y441), H811 (= H442)
binding fe2/s2 (inorganic) cluster: L715 (≠ I351)
binding uric acid: E773 (≠ S407)

Sites not aligning to the query:

active site: 851, 855, 883, 1231, 1232
binding bicarbonate ion: 880, 881, 885, 886, 889
binding flavin-adenine dinucleotide: 44, 227, 228, 229, 230, 231, 232, 233, 234, 307, 312, 317, 320, 321, 323, 324, 330, 373, 374
binding fe2/s2 (inorganic) cluster: 40, 41, 42, 44, 46, 47, 49, 69, 71, 109, 110, 111, 113, 145, 147
binding nicotinamide-adenine-dinucleotide: 327, 363, 364, 428, 430, 431, 471, 478
binding uric acid: 851, 885, 980, 981, 1049, 1050, 1232

4yswA Structure of rat xanthine oxidoreductase, c-terminal deletion protein variant, nadh bound form (see paper)
26% identity, 33% coverage: 202:447/748 of query aligns to 554:816/1286 of 4yswA

query
sites
4yswA

D

N

M

M

Q

Q

A

A

M

-

L

-

T

S

G

G

K

E

A

A

V

V

Y

Y

G

C

Y

D

D

D

I

I

-

P

Q

R

V

Y

D

E

N

N

M

E

L

L

Y

S

A

L

S

R

I

L

V

V

R

T

P

S

P

T

V

R

L

A

G

H

S

A

D

K

V

I

A

T

S

S

L

I

D

D

D

T

T

S

A

E

A

A

R

K

K

K

V

V

Q

P

G

G

V

F

V

V

D

C

V

F

Y

L

R

T

-

A

-

E

-

D

L

V

P

P

T

N

P

S

K

N

G

A

A

T

P

G

A

L

F

F

Q

N

-

D

-

E

-

T

-

V

-

F

-

A

-

K

-

D

-

E

-

V

-

T

A

C

L

V

G

G

G

H

V

I

-

I

-

G

A

A

V

V

A

A

T

D

N

T

T

P

W

E

S

H

A

A

L

Q

Q

R

G

A

R

A

K

R

A

G

L

V

K

K

V

I

T

T

W

Y

T

-

Q

-

S

-

A

-

N

-

S

E

S

D

H

L

D

P

S

A

K

I

T

I

Y

T

L

I

Q

Q

E

D

L

A

V

I

D

N

K

N

V

N

Q

S

A

F

P

Y

G

G

K

S

V

E

V

I

R

K

-

I

Q

E

V

K

G

G

D

D

E

L

V

K

K

K

A

G

W

F

P

S

E

E

A

A

-

D

N

N

T

V

L

V

K

S

A

G

I

E

Y

L

T

Y

V

I

P

G

Y

G

L

Q

I

E

H

H

S

F

S

Y

I
x
L

E

E

P

T

P

N

V

C

A

T

T

I

A

A

N

-

V

-

T

V

E

P

K

K

G

G

-

E

-

A

-

G

-

E

C

M

E

E

I

L

W

F

A

V

S

S

T

T

Q
|
Q

T

N

P

T

Q

M

S

K

T

T

Q

Q

Q

S

N

F

V

V

A

A

A

K

A

M

L

L

G

G

I

V

A

P

E

D

D

N

A

R

V

I

K

V

V

V

N

R

V

V

T

K

L

R

L

M

G

G

F

F

G

G

R

G

K

K

S
x
E

K

T

P

R

D

S

-

T

-

V

F

V

S

S

V

T

E

A

A

L

A

A

L

L

L

A

S

A

K

H

Q

K

L

T

K

G

R

R

P

P

V

V

K

R

V

C

S

M

W

L

S

D

R

R

-

D

E

E

D

D

E

M

I

L

Q

I

N

T

G

G

Y

G

Y
x
R

H
|
H

A

P

I

F

S

L

A

A

Q

K

active site: Q738 (= Q372), E773 (≠ S407)
binding bicarbonate ion: R810 (≠ Y441), H811 (= H442)
binding fe2/s2 (inorganic) cluster: L715 (≠ I351)
binding uric acid: E773 (≠ S407)

Sites not aligning to the query:

active site: 851, 855, 883, 1231, 1232
binding bicarbonate ion: 848, 880, 881, 882, 885, 886, 889
binding calcium ion: 838, 841, 842, 845, 878, 879
binding flavin-adenine dinucleotide: 44, 226, 227, 228, 229, 230, 231, 232, 233, 234, 307, 308, 312, 316, 317, 320, 321, 323, 324, 329, 330, 373, 374, 399
binding fe2/s2 (inorganic) cluster: 40, 41, 42, 44, 46, 47, 49, 71, 110, 111, 113, 145, 147
binding 1,4-dihydronicotinamide adenine dinucleotide: 233, 326, 327, 328, 363, 364, 400, 401, 428, 430, 431, 471, 478, 1196
binding uric acid: 851, 885, 980, 981, 1050, 1232

6a7xA Rat xanthine oxidoreductase, d428a variant, NAD bound form
26% identity, 33% coverage: 202:447/748 of query aligns to 556:818/1295 of 6a7xA

query
sites
6a7xA

D

N

M

M

Q

Q

A

A

M

-

L

-

T

S

G

G

K

E

A

A

V

V

Y

Y

G

C

Y

D

D

D

I

I

-

P

Q

R

V

Y

D

E

N

N

M

E

L

L

Y

S

A

L

S

R

I

L

V

V

R

T

P

S

P

T

V

R

L

A

G

H

S

A

D

K

V

I

A

T

S

S

L

I

D

D

D

T

T

S

A

E

A

A

R

K

K

K

V

V

Q

P

G

G

V

F

V

V

D

C

V

F

Y

L

R

T

-

A

-

E

-

D

L

V

P

P

T

N

P

S

K

N

G

A

A

T

P

G

A

L

F

F

Q

N

-

D

-

E

-

T

-

V

-

F

-

A

-

K

-

D

-

E

-

V

-

T

A

C

L

V

G

G

G

H

V

I

-

I

-

G

A

A

V

V

A

A

T

D

N

T

T

P

W

E

S

H

A

A

L

Q

Q

R

G

A

R

A

K

R

A

G

L

V

K

K

V

I

T

T

W

Y

T

-

Q

-

S

-

A

-

N

-

S

E

S

D

H

L

D

P

S

A

K

I

T

I

Y

T

L

I

Q

Q

E

D

L

A

V

I

D

N

K

N

V

N

Q

S

A

F

P

Y

G

G

K

S

V

E

V

I

R

K

-

I

Q

E

V

K

G

G

D

D

E

L

V

K

K

K

A

G

W

F

P

S

E

E

A

A

-

D

N

N

T

V

L

V

K

S

A

G

I

E

Y

L

T

Y

V

I

P

G

Y

G

L

Q

I

E

H

H

S

F

S

Y

I

L

E

E

P

T

P

N

V

C

A

T

T

I

A

A

N

-

V

-

T

V

E

P

K

K

G

G

-

E

-

A

-

G

-

E

C

M

E

E

I

L

W

F

A

V

S

S

T

T

Q
|
Q

T

N

P

T

Q

M

S

K

T

T

Q

Q

Q

S

N

F

V

V

A

A

A

K

A

M

L

L

G

G

I

V

A

P

E

D

D

N

A

R

V

I

K

V

V

V

N

R

V

V

T

K

L

R

L

M

G

G

F

F

G

G

R

G

K

K

S
x
E

K

T

P

R

D

S

-

T

-

V

F

V

S

S

V

T

E

A

A

L

A

A

L

L

L

A

S

A

K

H

Q

K

L

T

K

G

R

R

P

P

V

V

K

R

V

C

S

M

W

L

S

D

R

R

-

D

E

E

D

D

E

M

I

L

Q

I

N

T

G

G

Y

G

Y
x
R

H
|
H

A

P

I

F

S

L

A

A

Q

K

active site: Q740 (= Q372), E775 (≠ S407)
binding bicarbonate ion: R812 (≠ Y441), H813 (= H442)
binding uric acid: E775 (≠ S407)

Sites not aligning to the query:

active site: 853, 857, 885, 1233, 1234
binding bicarbonate ion: 850, 883, 884, 887, 888, 891
binding flavin-adenine dinucleotide: 44, 228, 229, 230, 231, 232, 233, 234, 235, 236, 309, 314, 319, 322, 323, 325, 326, 332, 376
binding fe2/s2 (inorganic) cluster: 40, 41, 42, 44, 46, 47, 49, 69, 71, 109, 110, 111, 113, 145, 147
binding nicotinamide-adenine-dinucleotide: 329, 365, 366, 432, 433, 473, 480
binding uric acid: 853, 887, 982, 983, 1052, 1234

P22985 Xanthine dehydrogenase/oxidase; EC 1.17.1.4; EC 1.17.3.2 from Rattus norvegicus (Rat) (see 2 papers)
26% identity, 33% coverage: 202:447/748 of query aligns to 583:845/1331 of P22985

query
sites
P22985

D

N

M

M

Q

Q

A

A

M

-

L

-

T

S

G

G

K

E

A

A

V

V

Y

Y

G

C

Y

D

D

D

I

I

-

P

Q

R

V

Y

D

E

N

N

M

E

L

L

Y

S

A

L

S

R

I

L

V

V

R

T

P

S

P

T

V

R

L

A

G

H

S

A

D

K

V

I

A

T

S

S

L

I

D

D

D

T

T

S

A

E

A

A

R

K

K

K

V

V

Q

P

G

G

V

F

V

V

D

C

V

F

Y

L

R

T

-

A

-

E

-

D

L

V

P

P

T

N

P

S

K

N

G

A

A

T

P

G

A

L

F

F

Q

N

-

D

-

E

-

T

-

V

-

F

-

A

-

K

-

D

-

E

-

V

-

T

A

C

L

V

G

G

G

H

V

I

-

I

-

G

A

A

V

V

A

A

T

D

N

T

T

P

W

E

S

H

A

A

L

Q

Q

R

G

A

R

A

K

R

A

G

L

V

K

K

V

I

T

T

W

Y

T

-

Q

-

S

-

A

-

N

-

S

E

S

D

H

L

D

P

S

A

K

I

T

I

Y

T

L

I

Q

Q

E

D

L

A

V

I

D

N

K

N

V

N

Q

S

A

F

P

Y

G

G

K

S

V

E

V

I

R

K

-

I

Q

E

V

K

G

G

D

D

E

L

V

K

K

K

A

G

W

F

P

S

E

E

A

A

-

D

N

N

T

V

L

V

K

S

A

G

I

E

Y

L

T

Y

V

I

P

G

Y

G

L

Q

I

E

H

H

S

F

S

Y

I

L

E

E

P

T

P

N

V

C

A

T

T

I

A

A

N

-

V

-

T

V

E

P

K

K

G

G

-

E

-

A

-

G

-

E

C

M

E

E

I

L

W

F

A

V

S

S

T

T

Q

Q

T

N

P

T

Q

M

S

K

T

T

Q

Q

Q

S

N

F

V

V

A

A

A

K

A

M

L

L

G

G

I

V

A

P

E

D

D

N

A

R

V

I

K

V

V

V

N

R

V

V

T

K

L

R

L

M

G

G

F

F

G

G

R

G

K

K

S

E

K

T

P

R

D

S

-

T

-

V

F

V

S

S

V

T

E

A

A

L

A

A

L

L

L

A

S

A

K

H

Q

K

L

T

K

G

R

R

P

P

V

V

K

R

V

C

S

M

W

L

S

D

R

R

-

D

E

E

D

D

E

M

I

L

Q

I

N

T

G

G

Y

G

Y

R

H

H

A

P

I

F

S

L

A

A

Q

K

Sites not aligning to the query:

43 binding
48 binding
51 binding
73 binding
112 binding
115 binding
147 binding
149 binding
256:263 binding
335:336 WF→AL: Converts the enzyme to the oxidase form that utilizes molecular oxygen as electron acceptor. Interferes with normal conversion to the dehydrogenase form by reducing agents.
346:350 binding
359 binding
403 binding
535 C→A: Slows the conversion from the dehydrogenase form to the oxidase form; when associated with R-992. Abolishes conversion from the dehydrogenase form to the oxidase form; when associated with R-992 and S-1316.
992 C→R: Slows the conversion from the dehydrogenase form to the oxidase form; when associated with A-535. Abolishes conversion from the dehydrogenase form to the oxidase form; when associated with A-535 and S-1316.
1316 C→S: Abolishes conversion from the dehydrogenase form to the oxidase form; when associated with A-535 and R-992.

Q8GUQ8 Xanthine dehydrogenase 1; AtXDH1; EC 1.17.1.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
24% identity, 37% coverage: 186:462/748 of query aligns to 590:888/1361 of Q8GUQ8

query
sites
Q8GUQ8

K

E

A

T

V

V

K

K

D

Q

F

G

K

T

Q

S

I

V

G

G

A

S

S

S

R

E

Q

V

I

H

V

L

D

S

M

A

Q

R

A

M

M

Q

L

V

T

T

G

G

K

E

A

A

V

E

Y

Y

G

T

Y

D

D

D

I

T

Q

P

V

V

D

P

-

P

N

N

M

T

L

L

Y

H

A

A

S

A

I

F

V

V

R

L

P

S

P

K

V

V

L

P

G

H

S

A

D

R

V

I

A

L

S

S

L

I

D

D

T

S

A

A

R

K

K

S

V

S

Q

S

G

G

V

F

V

V

D

G

V

L

Y

F

R

-

L

-

P

-

T

L

P

A

K

K

G

D

A

I

P

P

A

G

F

D

Q

N

A

M

L

I

G

G

-

P

-

I

-

V

-

P

-

D

-

E

-

L

-

F

-

A

-

T

-

D

-

V

-

T

-

C

-

V

-

G

-

Q

-

V

-

I

G

G

V

V

A

V

V

V

V

A

A

D

T

T

N

H

T

E

W

N

S

A

A

K

L

T

Q

A

G

A

R

G

K

K

A

V

-

D

-

V

-

R

-

Y

-

E

-

L

-

P

-

A

-

I

L

L

K

S

V

I

T

K

W

E

T

A

Q

I

S

N

A

A

N

K

S

S

S

F

H

H

D

P

S

N

-

T

-

E

K

K

T

R

Y

L

L

R

Q

K

E

G

L

D

V

V

D

E

K

L

V

C

Q

F

A

Q

P

S

G

G

K

Q

V

C

V

D

R

R

Q

V

V

I

G

E

D

G

E

E

V

V

K

Q

A

M

W

G

P

G

E

Q

A

E

N

-

T

-

L

-

K

-

A

-

I

-

Y

-

T

-

V

-

P

-

Y

-

L

-

I

-

H

H

S

F

S

Y

I

L

E

E

P

P

P

N

V

G

A

S

T

L

A

V

N

W

V

T

T

V

E

D

K

G

G

G

C

S

E

E

I

V

W

H

-

M

-

I

A

S

S

S

T

T

Q

Q

T

A

P

P

Q

Q

S

K

T

H

Q

Q

Q

K

N

Y

V

V

A

S

A

H

A

V

L

L

G

G

I

L

A

P

E

M

D

S

A

K

V

V

K

V

V

C

N

K

V

T

T

K

L

R

L

I

G

G

F

F

-

G

G

G

R

K

K
x
E

S

T

K

R

P

S

D

A

F

F

S

I

V

A

E

A

A

A

L

S

L

V

-

P

S

S

K

Y

Q

L

L

L

K

N

R

R

P

P

V

V

K

K

V

L

S

I

W

L

S

D

R

R

E

D

-

V

D

D

E

M

I

M

Q

I

N

T

G

G

Y

H

Y

R

H

H

A

S

I

F

S

L

A

G

Q

K

C

-

Y

Y

Q

K

A

V

L

G

F

F

D

T

T

N

N

E

N

G

Q

K

P

I

T

L

A

A

L

L

E831 (≠ K406) mutation to A: Loss of activity.

Sites not aligning to the query:

364 W→A: Decreases activity 8-fold.
421 Y→A: Decreases activity 4-fold.
909 R→A: Decreases activity 12-fold.
1297 E→A: Decreases activity 40-fold.

Query Sequence

>202167 SO3048 isoquinoline 1-oxidoreductase, beta subunit, putative (NCBI ptt file)
MSTFTAVENISRRDVLKLFGAVGGGLALGASGLSWSPMALAQDQQARLNLFIAIGEDDKV
YLTCHRSEMGQGIRTGILQILAEELEADWDKIVPIQGLADKRYASQNTDGSRSIRENYDR
MREMGAMARTMLEQAAAARWKVPVTEVQAKDHKVMHAKSGRSARFGELALDAAALPLPAA
DSLRLKAVKDFKQIGASRQIVDMQAMLTGKAVYGYDIQVDNMLYASIVRPPVLGSDVASL
DDTAARKVQGVVDVYRLPTPKGAPAFQALGGVAVVATNTWSALQGRKALKVTWTQSANSS
HDSKTYLQELVDKVQAPGKVVRQVGDEVKAWPEANTLKAIYTVPYLIHSSIEPPVATANV
TEKGCEIWASTQTPQSTQQNVAAALGIAEDAVKVNVTLLGGGFGRKSKPDFSVEAALLSK
QLKRPVKVSWSREDEIQNGYYHAISAQCYQALFDTNNQPTALLARTGFPSISSTFAEGVE
YPSDGELDLGFVDVPFALPHLRYEAVKATAHSRIGWMRSVCNIQHGFGVGSFVDEMAHKA
QLSCPDMWRSLLGQPRRETFDNQGFKYGNYGEELTRHPVDIGRYLKVIEAVEQAQAKAPK
AGKNQGWGFAVHRSFVSVVAVAMRVEVSEDKQLKVLNAIAAIDAGTVVNPDRVKAQTEGA
IVFGLSLALMGEISYQEGKVVQSNFHDYPLLRLPQVPEIEIIIIDSDAPPAGVGEPGVPP
VAPSLTNAIFAATGVRIRDLPVNKQLKV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory