SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 202262 FitnessBrowser__MR1:202262 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3vbkC Crystal structure of the s84a mutant of antd, an n-acyltransferase from bacillus cereus in complex with dtdp-4-amino-4,6-dideoxyglucose and coenzyme a (see paper)
31% identity, 78% coverage: 46:209/209 of query aligns to 7:174/186 of 3vbkC

query
sites
3vbkC

Q

Q

T

E

N

E

L

L

C

K

A

K

L

I

E

G

T

F

V

L

T

S

I

V

G

G

D

K

N

N

C

V

F

L

I

I

A

S

P

K

E

K

A

A

Q

S

L

I

F
x
Y

A

-

E

N

P

P

N

G

R

V

D

-

I

I

N

S

I

I

G

G

N

N

H

N

C

V

M
x
R

I

I

A
x
D

D

F

C

C

F

I

L

L

H
x
S

G

G

P

K

I

V

T

T

L

I

G

G

N

S

E

Y

V

S

A
x
H

I

I

N

A

H

A

G

Y

C

T

S

A

L

L

D

Y

G

G

R

E

V

V

G

G

I

I

Q

E

I

M

G

Y

D

D

Q

F

T

A

R

N

I
|
I

A

A

N

S

H

R

V

T

T

I

I

V

Y
|
Y

A

A

F
x
A

-
x
I

-

D

-
x
F

-

S

-

G

N

N

H

A

G

L

M

M

A

G

P

P

D

T

T

I

P

P

I

N

Y

Q

Q

Y

Q

K

A

N

S

V

H

K

S

T

K

G

G

K

I

V

V

I

I

L

G

K

K

K

D

H

V

V

W

I

I

I

G

G

A

A

Q

H

A

S

G

I

I

I

V
x
F

D

P

G

N

V

V

T

V

I

I

G

G

D

E

H

G

A

V

V

A

I

V

G
|
G

M
x
A

G

M

C

S

I
x
M

V

V

T
x
K

K

E

D

S

V

L

P

D

A

D

W

W

A

Y

I

I

V

Y

A
x
V

G

G

N
x
V

P

P

A

V

R

R

V

K

I

I

G

K

D

A

R

R

R

K

D

R

K

K

binding dTDP-4-amino-4,6-dideoxyglucose: Y31 (≠ F70), R43 (≠ M84), D45 (≠ A86), D46 (≠ A87), S51 (≠ H92), H61 (≠ A102), A92 (≠ F133)
binding bicarbonate ion: H61 (≠ A102), I83 (= I124)
binding coenzyme a: Y90 (= Y131), I93 (vs. gap), F96 (vs. gap), F133 (≠ V168), G145 (= G180), A146 (≠ M181), M149 (≠ I184), K151 (≠ T186), V161 (≠ A196), V163 (≠ N198)

Sites not aligning to the query:

binding dTDP-4-amino-4,6-dideoxyglucose: 2

3vbjA Crystal structure of antd, an n-acyltransferase from bacillus cereus in complex with dtdp and 3-hydroxybutyryl-coa (see paper)
30% identity, 78% coverage: 46:209/209 of query aligns to 7:174/186 of 3vbjA

query
sites
3vbjA

Q

Q

T

E

N

E

L

L

C

K

A

K

L

I

E

G

T

F

V

L

T

S

I

V

G

G

D

K

N

N

C

V

F

L

I

I

A

S

P

K

E

K

A

A

Q

S

L

I

F
x
Y

A

-

E

N

P

P

N

G

R

V

D

-

I

I

N

S

I

I

G

G

N

N

H

N

C

V

M
x
R

I

I

A
x
D

D

F

C

C

F

I

L

L

H
x
S

G

G

P

K

I

V

T

T

L

I

G

G

N

S

E

Y

V

S

A
x
H

I

I

N

A

H

A

G

Y

C

T

S

A

L

L

D

Y

G

G

R

E

V

V

G

G

I

I

Q

E

I

M

G

Y

D

D

Q

F

T

A

R

N

I
|
I

A
x
S

N

S

H

R

V

T

T

I

I

V

Y
|
Y

A

A

F

A

-

I

-
x
D

-

D

-
x
F

-

S

-

G

N

N

H

A

G

L

M

M

A

G

P

P

D

T

T

I

P

P

I

N

Y

Q

Q

Y

Q

K

A

N

S

V

H

K

S

T

K

G

G

K

I

V

V

I

I

L

G

K

K

K

D

H

V

V

W

I

I

I

G

G

A

A

Q

H

A

S

G

I

I

I

V
x
F

D

P

G

N

V

V

T

V

I

I

G

G

D

E

H

G

A

V

V

A

I

V

G
|
G

M
x
A

G

M

C

S

I

M

V

V

T
x
K

K

E

D

S

V

L

P

D

A

D

W

W

A

Y

I

I

V

Y

A
x
V

G

G

N
x
V

P

P

A

V

R

R

V

K

I

I

G
x
K

D

A

R

R

R

K

D

R

K

K

binding 3-hydroxybutanoyl-coenzyme a: H61 (≠ A102), I83 (= I124), S84 (≠ A125), Y90 (= Y131), D94 (vs. gap), F96 (vs. gap), F133 (≠ V168), G145 (= G180), A146 (≠ M181), K151 (≠ T186), V161 (≠ A196), V163 (≠ N198), K169 (≠ G204)
binding thymidine-5'-diphosphate: Y31 (≠ F70), R43 (≠ M84), D45 (≠ A86), D46 (≠ A87), S51 (≠ H92)

Sites not aligning to the query:

binding thymidine-5'-diphosphate: 4

3vbiA Crystal structure of antd, an n-acyltransferase from bacillus cereus in complex with dtdp-4-amino-4,6-dideoxyglucose and coenzyme a (see paper)
30% identity, 78% coverage: 46:209/209 of query aligns to 7:174/186 of 3vbiA

query
sites
3vbiA

Q

Q

T

E

N

E

L

L

C

K

A

K

L

I

E

G

T

F

V

L

T

S

I

V

G

G

D

K

N

N

C

V

F

L

I

I

A

S

P

K

E

K

A

A

Q

S

L

I

F
x
Y

A

-

E

N

P

P

N

G

R

V

D

-

I

I

N

S

I

I

G

G

N

N

H

N

C

V

M
x
R

I

I

A
x
D

D

F

C

C

F

I

L

L

H
x
S

G

G

P

K

I

V

T

T

L

I

G

G

N

S

E

Y

V

S

A
x
H

I

I

N

A

H

A

G

Y

C

T

S

A

L

L

D
x
Y

G

G

R

E

V

V

G

G

I

I

Q

E

I

M

G

Y

D

D

Q

F

T

A

R

N

I

I

A
x
S

N

S

H

R

V

T

T

I

I

V

Y
|
Y

A

A

F
x
A

-
x
I

-
x
D

-

D

-
x
F

-

S

-

G

N

N

H

A

G

L

M

M

A

G

P

P

D

T

T

I

P

P

I

N

Y

Q

Q

Y

Q

K

A

N

S

V

H

K

S

T

K

G

G

K

I

V

V

I

I

L

G

K

K

K

D

H

V

V

W

I

I

I

G

G

A

A

Q

H

A

S

G

I

I

I

V
x
F

D

P

G

N

V

V

T

V

I

I

G

G

D

E

H

G

A

V

V

A

I

V

G
|
G

M
x
A

G

M

C

S

I

M

V

V

T
x
K

K

E

D

S

V

L

P

D

A

D

W

W

A

Y

I

I

V

Y

A
x
V

G

G

N
x
V

P

P

A

V

R

R

V

K

I

I

G
x
K

D

A

R

R

R

K

D

R

K

K

binding dTDP-4-amino-4,6-dideoxyglucose: Y31 (≠ F70), R43 (≠ M84), D45 (≠ A86), D46 (≠ A87), S51 (≠ H92), H61 (≠ A102), Y69 (≠ D110), A92 (≠ F133), D94 (vs. gap)
binding coenzyme a: S84 (≠ A125), Y90 (= Y131), I93 (vs. gap), F96 (vs. gap), F133 (≠ V168), G145 (= G180), A146 (≠ M181), K151 (≠ T186), V161 (≠ A196), V163 (≠ N198), K169 (≠ G204)

Sites not aligning to the query:

binding dTDP-4-amino-4,6-dideoxyglucose: 2, 3

3vbmE Crystal structure of the s84t mutant of antd, an n-acyltransferase from bacillus cereus in complex with dtdp and coenzyme a (see paper)
30% identity, 78% coverage: 46:209/209 of query aligns to 14:181/193 of 3vbmE

query
sites
3vbmE

Q

Q

T

E

N

E

L

L

C

K

A

K

L

I

E

G

T

F

V

L

T

S

I

V

G

G

D

K

N

N

C

V

F

L

I

I

A

S

P

K

E

K

A

A

Q

S

L

I

F
x
Y

A

-

E

N

P

P

N

G

R

V

D

-

I

I

N

S

I

I

G

G

N

N

H

N

C

V

M
x
R

I

I

A
x
D

D

F

C

C

F

I

L

L

H
x
S

G

G

P

K

I

V

T

T

L

I

G

G

N

S

E

Y

V

S

A

H

I

I

N

A

H

A

G

Y

C

T

S

A

L

L

D

Y

G

G

R

E

V

V

G

G

I

I

Q

E

I

M

G

Y

D

D

Q

F

T

A

R

N

I

I

A
x
T

N

S

H

R

V

T

T

I

I

V

Y
|
Y

A

A

F

A

-
x
I

-

D

-
x
F

-

S

-

G

N

N

H

A

G

L

M

M

A

G

P

P

D

T

T

I

P

P

I

N

Y

Q

Q

Y

Q

K

A

N

S

V

H

K

S

T

K

G

G

K

I

V

V

I

I

L

G

K

K

K

D

H

V

V

W

I

I

I

G
|
G

A

A

Q

H

A

S

G

I

I

I

V
x
F

D

P

G

N

V

V

T

V

I

I

G

G

D

E

H

G

A

V

V

A

I

V

G
|
G

M
x
A

G

M

C

S

I

M

V

V

T
x
K

K

E

D

S

V

L

P

D

A

D

W

W

A

Y

I

I

V

Y

A
x
V

G

G

N
x
V

P
|
P

A

V

R

R

V

K

I

I

G
x
K

D

A

R

R

R

K

D

R

K

K

binding coenzyme a: T91 (≠ A125), Y97 (= Y131), I100 (vs. gap), F103 (vs. gap), G134 (= G162), F140 (≠ V168), G152 (= G180), A153 (≠ M181), K158 (≠ T186), V168 (≠ A196), V170 (≠ N198), P171 (= P199), K176 (≠ G204)
binding thymidine-5'-diphosphate: Y38 (≠ F70), R50 (≠ M84), D52 (≠ A86), D53 (≠ A87), S58 (≠ H92)

Sites not aligning to the query:

binding thymidine-5'-diphosphate: 9

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
28% identity, 92% coverage: 17:209/209 of query aligns to 23:187/201 of 1krvA

query
sites
1krvA

K

E

K

K

R
|
R

L

L

S

R

W

G

M

K

P

T

W

L

L

M

Y

Y

F

-

S

-

L

-

K

-

E

-

K

-

H

E

L

F

T

N

W

H

A

S

K

H

P

P

W

S

Q

E

N

V

E

E

V

K

Q

R

T

E

N

S

L

L

C

I

A

K

L

E

E

M

T

F

V

A

T

T

I

V

G

G

D

E

N

N

C

A

F

W

I

V

A

E

P

P

E

P

A

V

Q

Y

L

F

F

-

A

-

E

-

P

-

N

-

R

-

D

-

I

-

N
x
S

I

Y

G

G

N

S

H

N

C

I

M

H

I

I

A

G

A

R

D

N

C

F

F
x
Y

L

A

H
x
N

G

F

P

N

I

L

T

T

L

I

G
x
V

N

D

E
x
D

V

Y

A

T

I

V

N

-

H

-

G

-

C

-

S

-

L

-

D

-

G

-

R

-

V

-

G

-

I

-

Q

T

I

I

G

G

D

D

Q

N

T

V

R

L

I

I

A
|
A

N

P

H

N

V

V

T

T

I

L

Y
x
S

A

V

F
x
T

N

G

H

H

G

-

M

-

A

-

P

-

D

-

T

-

P

P

I

V

Y

H

Q

H

Q

E

A

L

S

R

-

K

-

N

-

G

-

E

-
x
M

H

Y

S

S

K

F

G

P

I

I

V

T

I

I

G

G

K

N

D

N

V

V

W
|
W

I

I

G
|
G

A
x
S

Q

H

A

V

G

V

I

I

V
x
N

D

P

G

G

V

V

T

T

I

I

G

G

D

D

H

N

A

S

V

V

I

I

G

G

M
x
A

G

G

C

S

I

I

V

V

T

T

K

K

D

D

V

I

P

P

A

P

W

N

A

V

I

V

V

A

A

A

G

G

N

V

P
|
P

A

C

R
|
R

V

V

I

I

G
x
R

D

E

R

I

R

N

D

D

K

R

binding 4-nitrophenyl beta-D-galactopyranoside: R25 (= R19), S70 (≠ N78), Y82 (≠ F90), N84 (≠ H92), V90 (≠ G98), D92 (≠ E100), M126 (vs. gap)
binding coenzyme a: A104 (= A125), S110 (≠ Y131), T112 (≠ F133), W138 (= W160), G140 (= G162), S141 (≠ A163), N146 (≠ V168), A159 (≠ M181), P177 (= P199), R179 (= R201), R182 (≠ G204)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
28% identity, 92% coverage: 17:209/209 of query aligns to 23:187/201 of 1kruA

query
sites
1kruA

K

E

K

K

R
|
R

L

L

S

R

W

G

M

K

P

T

W

L

L

M

Y

Y

F

-

S

-

L

-

K

-

E

-

K

-

H

E

L

F

T

N

W

H

A

S

K

H

P

P

W

S

Q

E

N

V

E

E

V

K

Q

R

T

E

N

S

L

L

C

I

A

K

L

E

E

M

T

F

V

A

T

T

I

V

G

G

D

E

N

N

C

A

F
x
W

I

V

A

E

P

P

E

P

A

V

Q

Y

L

F

F

-

A

-

E

-

P

-

N

-

R

-

D

-

I

-

N
x
S

I

Y

G

G

N

S

H

N

C

I

M

H

I

I

A

G

A

R

D

N

C

F

F
x
Y

L

A

H
x
N

G

F

P

N

I

L

T

T

L

I

G
x
V

N

D

E
x
D

V

Y

A

T

I

V

N

-

H

-

G

-

C

-

S

-

L

-

D

-

G

-

R

-

V

-

G

-

I

-

Q

T

I

I

G

G

D

D

Q

N

T

V

R
x
L

I

I

A

A

N

P

H

N

V

V

T

T

I

L

Y

S

A

V

F

T

N

G

H
|
H

G

-

M

-

A

-

P

-

D

-

T

-

P

P

I

V

Y

H

Q

H

Q

E

A

L

S

R

-

K

-

N

-

G

-

E

-
x
M

H

Y

S

S

K

F

G

P

I

I

V

T

I

I

G

G

K

N

D

N

V

V

W
|
W

I

I

G
|
G

A
x
S

Q

H

A

V

G

V

I

I

V

N

D

P

G

G

V

V

T

T

I

I

G

G

D

D

H

N

A

S

V

V

I

I

G
|
G

M
x
A

G

G

C

S

I

I

V

V

T

T

K

K

D

D

V

I

P

P

A

P

W

N

A

V

I

V

V

A

A

A

G

G

N

V

P
|
P

A

C

R

R

V

V

I

I

G
x
R

D

E

R

I

R

N

D

D

K

R

binding coenzyme a: H114 (= H135), W138 (= W160), G140 (= G162), S141 (≠ A163), G158 (= G180), A159 (≠ M181), P177 (= P199), R182 (≠ G204)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: R25 (= R19), W62 (≠ F62), S70 (≠ N78), Y82 (≠ F90), Y82 (≠ F90), N84 (≠ H92), V90 (≠ G98), D92 (≠ E100), L102 (≠ R123), H114 (= H135), M126 (vs. gap)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
28% identity, 92% coverage: 17:209/209 of query aligns to 23:187/200 of 1krrA

query
sites
1krrA

K

E

K

K

R

R

L

L

S

R

W

G

M

K

P

T

W

L

L

M

Y

Y

F

-

S

-

L

-

K

-

E

-

K

-

H

E

L

F

T

N

W

H

A

S

K

H

P

P

W

S

Q

E

N

V

E

E

V

K

Q

R

T

E

N

S

L

L

C

I

A

K

L

E

E

M

T

F

V

A

T

T

I

V

G

G

D

E

N

N

C

A

F

W

I

V

A

E

P

P

E

P

A

V

Q

Y

L

F

F

-

A

-

E

-

P

-

N

-

R

-

D

-

I

-

N

S

I

Y

G

G

N

S

H

N

C

I

M

H

I

I

A

G

A

R

D

N

C

F

F

Y

L

A

H
x
N

G

F

P

N

I

L

T

T

L

I

G

V

N

D

E

D

V

Y

A

T

I

V

N

-

H

-

G

-

C

-

S

-

L

-

D

-

G

-

R

-

V

-

G

-

I

-

Q

T

I

I

G

G

D

D

Q

N

T

V

R

L

I
|
I

A
|
A

N
x
P

H

N

V

V

T

T

I

L

Y

S

A

V

F
x
T

N

G

H
|
H

G

-

M

-

A

-

P

-

D

-

T

-

P

P

I

V

Y

H

Q

H

Q

E

A

L

S

R

-

K

-

N

-

G

-

E

-

M

H

Y

S

S

K

F

G

P

I

I

V

T

I

I

G

G

K

N

D

N

V

V

W

W

I

I

G
|
G

A
x
S

Q

H

A

V

G

V

I

I

V
x
N

D

P

G

G

V

V

T

T

I

I

G

G

D

D

H

N

A

S

V

V

I

I

G
|
G

M
x
A

G

G

C

S

I

I

V

V

T

T

K

K

D

D

V

I

P

P

A

P

W

N

A

V

I

V

V

A

A
|
A

G

G

N

V

P
|
P

A

C

R

R

V

V

I

I

G
x
R

D

E

R

I

R

N

D

D

K

R

binding acetyl coenzyme *a: N84 (≠ H92), I103 (= I124), A104 (= A125), P105 (≠ N126), T112 (≠ F133), H114 (= H135), G140 (= G162), S141 (≠ A163), N146 (≠ V168), G158 (= G180), A159 (≠ M181), A174 (= A196), P177 (= P199), R182 (≠ G204)

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
28% identity, 92% coverage: 17:209/209 of query aligns to 24:188/203 of P07464

query
sites
P07464

K

E

K

K

R

R

L

L

S

R

W

G

M

K

P

T

W

L

L

M

Y

Y

F

-

S

-

L

-

K

-

E

-

K

-

H

E

L

F

T

N

W

H

A

S

K

H

P

P

W

S

Q

E

N

V

E

E

V

K

Q

R

T

E

N

S

L

L

C

I

A

K

L

E

E

M

T

F

V

A

T

T

I

V

G

G

D

E

N

N

C

A

F

W

I

V

A

E

P

P

E

P

A

V

Q

Y

L

F

F

-

A

-

E

-

P

-

N

-

R

-

D

-

I

-

N
x
S

I

Y

G

G

N

S

H

N

C

I

M

H

I

I

A

G

A

R

D

N

C

F

F

Y

L

A

H
x
N

G

F

P

N

I

L

T

T

L

I

G

V

N

D

E
x
D

V

Y

A

T

I

V

N

-

H

-

G

-

C

-

S

-

L

-

D

-

G

-

R

-

V

-

G

-

I

-

Q

T

I

I

G

G

D

D

Q

N

T

V

R

L

I

I

A

A

N

P

H

N

V

V

T

T

I

L

Y

S

A

V

F

T

N

G

H
|
H

G

-

M

-

A

-

P

-

D

-

T

-

P

P

I

V

Y

H

Q

H

Q

E

A

L

S

R

-

K

-

N

-

G

-

E

-

M

H

Y

S

S

K

F

G

P

I

I

V

T

I

I

G

G

K

N

D

N

V

V

W

W

I

I

G

G

A
x
S

Q

H

A

V

G

V

I

I

V

N

D

P

G

G

V

V

T

T

I

I

G

G

D

D

H

N

A

S

V

V

I

I

G

G

M
x
A

G

G

C

S

I

I

V

V

T
|
T

K
|
K

D

D

V

I

P

P

A

P

W

N

A

V

I

V

V

A

A

A

G

G

N

V

P

P

A

C

R
|
R

V

V

I

I

G
x
R

D

E

R

I

R

N

D

D

K

R

S71 (≠ N78) binding
N85 (≠ H92) binding in other chain; binding in other chain
D93 (≠ E100) binding
H115 (= H135) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A163) binding in other chain
A160 (≠ M181) binding in other chain
TK 165:166 (= TK 186:187) binding
R180 (= R201) binding
R183 (≠ G204) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding

4isxA The crystal structure of maltose o-acetyltransferase from clostridium difficile 630 in complex with acetyl-coa
37% identity, 65% coverage: 68:203/209 of query aligns to 52:181/186 of 4isxA

query
sites
4isxA

Q

Q

L

L

F

F

A

G

E

S

P

V

N

G

R

K

D

Q

I

I

N

N

I

V

G

E

N

Q

H

N

C

I

M

R

I

-

A

-

D

-

C

C

F

D

L

Y

H

G

G

Y

P

N

I

I

T

H

L

V

G

G

N

E

E

N

V

F

A

F

I

A

N
|
N

H

Y

G

D

C

C

-

I

S

F

L

L

D

D

G

V

G

C

R

K

V

-

G

-

I

I

Q

E

I

I

G

G

D

D

Q

N

T

V

R

M

I

L

A
|
A

N

P

H

N

V

V

T

Q

I

I

Y

Y

A

T

F

A

N

Y

H

H

G

-

M

-

A

-

P

-

D

-

T

-

P

P

I

I

Y

D

Q

A

Q

Q

A

L

S

R

H

N

S

S

-

G

-

I

-

E

-

Y

-

G

K

S

G

P

I

V

V

K

I

I

G

G

K

D

D

N

V

V

W
|
W

I

I

G
|
G

A
x
G

Q

G

A

V

G

I

I

I

V

T

D

P

G

G

V

I

T

T

I

I

G

G

D

D

H

N

A

V

V

V

I

I

G

G

M
x
A

G

G

C

S

I

V

V

V

T

T

K

K

D

D

V

I

P

P

A

P

W

N

A

T

I

V

V

A

A
x
V

G

G

N

N

P

P

A

C

R

R

V

V

I

I

binding acetyl coenzyme *a: N84 (= N104), A104 (= A125), W138 (= W160), G140 (= G162), G141 (≠ A163), A159 (≠ M181), V174 (≠ A196)

Sites not aligning to the query:

binding acetyl coenzyme *a: 182

3igjC Crystal structure of maltose o-acetyltransferase complexed with acetyl coenzyme a from bacillus anthracis
44% identity, 42% coverage: 116:203/209 of query aligns to 97:183/188 of 3igjC

query
sites
3igjC

I

V

Q

R

I

I

G

G

D

D

Q

H

T

C

R

M

I

F

A
|
A

N

P

H

G

V

V

T

H

I

I

Y
|
Y

A

T

F
x
A

N

T

H
|
H

G

-

M

-

A

-

P

P

D

L

T

H

P

P

I

V

Y

E

Q

R

Q

N

A

S

-

G

-

K

S

E

H

Y

S

G

K

K

G

P

I

V

V

K

I

I

G

G

K

N

D

N

V

V

W

W

I

V

G
|
G

A

G

Q

G

A

A

G

I

I

I

V
x
N

D

P

G

G

V

V

T

S

I

I

G

G

D

D

H

N

A

A

V

V

I

I

G
x
A

M
x
S

G

G

C

A

I

V

V

V

T
|
T

K

K

D

D

V

V

P

P

A

N

W

N

A

V

I

V

V

V

A

G

G

G

N
|
N

P

P

A

A

R

K

V

V

I

I

binding acetyl coenzyme *a: A106 (= A125), Y112 (= Y131), A114 (≠ F133), H116 (= H135), G142 (= G162), N148 (≠ V168), A160 (≠ G180), S161 (≠ M181), T166 (= T186), N178 (= N198)

Sites not aligning to the query:

binding acetyl coenzyme *a: 84

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
29% identity, 83% coverage: 30:203/209 of query aligns to 1:181/190 of 5u2kA

query
sites
5u2kA

L

M

K

T

E

E

K

K

H

E

L

K

T

M

W

L

A

A

K

E

P

K

W

W

Q

Y

N

D

E

A

V

N

Q

F

T

D

N

Q

L

Y

C

L

A

I

L

N

E

E

T

R

V

A

T

R

I

A

G

K

D

D

N

I

C

C

F

F

-

E

-

L

-

N

-

H

I

T

A

R

P

P

E

S

A

A

-

T

-

N

-

K

-

R

-

K

-

E

-

L

-

I

-

D

Q

Q

L

L

F

F

A

Q

E

T

P

T

N

T

R

D

D

N

I

V

N

S

I

I

G

S

N

-

H

-

C

-

M

-

I

I

A

P

A

F

D

D

C

T

F

D

L

Y

H

G

G

W

P

N

I

V

T

K

L

L

G

G

N

K

E

N

V

V

A

Y

I

V

N

N

H

T

G

N

C

C

S

Y

-

F

L
x
M

D

D

G

G

R

Q

V

-

G

-

I

I

Q

T

I

I

G

G

D

D

Q

N

T

V

R

F

I

I

A

G

N

P

H

N

V

C

T

G

I

F

Y
|
Y

A

T

F
x
A

N

T

H
|
H

G

-

M

-

A

-

P

P

D

L

T

N

P

F

I

H

Y

H

Q

R

Q

N

A

E

S

G

H

F

S

E

K

K

G

A

-

G

-

P

I

I

V

H

I

I

G

G

K

S

D

N

V

T

W

W

I

F

G

G

A

G

Q

H

A

V

G

A

I

V

V
x
L

D

P

G

G

V

V

T

T

I

I

G

G

D

E

H

G

A

S

V

V

I

I

G

G

M

A

G

G

C

S

I

V

V

V

T
|
T

K
|
K

D

D

V

I

P

P

A

P

W

H

A

S

I

L

V

A

A

V

G

G

N

N

P

P

A

C

R

K

V

V

I

V

binding coenzyme a: M90 (≠ L109), Y110 (= Y131), A112 (≠ F133), H114 (= H135), L146 (≠ V168), T164 (= T186), K165 (= K187)

A1ADJ6 Polysialic acid O-acetyltransferase; Capsule O-acetyl transferase; EC 2.3.1.136 from Escherichia coli O1:K1 / APEC (see paper)
31% identity, 65% coverage: 69:203/209 of query aligns to 141:277/307 of A1ADJ6

query
sites
A1ADJ6

L

I

F

F

A

R

E

G

P

D

N

N

R

N

D

Y

I

V

N

R

I

I

G

H

N

K

H

N

C

S

M

K

I

I

A

K

A

G

D

D

C

I

F

V

L

A

H

T

-

K

-

G

G

S

P

K

I

V

T

I

L

I

G

G

N

R

E

R

V

T

A

T

I

I

N

G

H

A

G

G

C

F

S

E

L

V

D

V

G

T

G

D

R

K

V

C

G

N

I

V

Q

T

I

I

G

G

D

H

Q

D

T

C

R

M

I

I

A

A

N

R

H

D

V

V

T

I

I

L

Y

R

A

A

F

S

N

D

H

G

G
x
H

M

P

A

I

P

F

D

D

T

I

P

H

I

S

Y

K

Q

K

Q

R

A

I

S

N

H

W

S

A

K

K

G

D

I

I

V

I

I

I

G

S

K

S

D

Y

V

V

W
|
W

I

V

G

G

A

R

Q

N

A

V

G

S

I

I

V

M

D

K

G

G

V

V

T

S

I

V

G

G

D

S

H

G

A

S

V

V

I

I

G

G

M

Y

G

G

C

S

I

I

V

V

T

T

K

K

D

D

V

V

P

P

A

S

W

M

A

C

I

A

V

A

A

A

G

G

N

N

P

P

A

A

R

K

V

I

I

I

H210 (≠ G136) mutation to A: Loss of activity.
W234 (= W160) mutation to A: Loss of activity.

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
34% identity, 60% coverage: 79:204/209 of query aligns to 31:142/294 of 4mzuF

query
sites
4mzuF

I

I

G

G

N

R

H

N

C

C

M

N

I

I

A
x
C

A

A

D

N

C

S

F

L

L

I

H

E

G

N

P

D

I

V

T

V

L

I

G

G

N

D

E

N

V

V

A

T

I

I

N

K

H

S

G

G

C

V

S

Q

L

I

-

W

D

D

G

G

G

-

R

-

V

-

G

-

I

I

Q

H

I

I

G

Q

D

D

Q

D

T

V

R

F

I

I

A

G

N

P

H

N

V

V

T

T

I

-

Y

-

A

-

F

F

N

T

H
x
N

G
x
D

M
x
K

A

Q

P

P

D

R

T

S

P

K

I

I

Y

-

Q

-

A

-

S

-

H

-

S

-

K

-

G

K

I

T

V

I

I

V

G

K

K

K

D

G

V

A

W

S

I

I

G
|
G

A
|
A

Q

N

A

S

G

T

I

I

V
x
L

D

P

G

G

V

I

T

L

I

I

G

G

D

E

H

N

A

A

V

M

I

V

G
|
G

M
x
A

G

G

C

A

I

V

V

I

T
|
T

K
|
K

D

N

V

V

P

P

A

D

W

N

A

A

I

I

V

V

A
x
I

G

G

N
|
N

P

P

A

G

R
|
R

V

I

I

T

G

G

binding coenzyme a: N81 (≠ H135), D82 (≠ G136), K83 (≠ M137), G100 (= G162), A101 (= A163), L106 (≠ V168), G118 (= G180), A119 (≠ M181), T124 (= T186), K125 (= K187), I134 (≠ A196), N136 (= N198), R139 (= R201)
binding thymine: C38 (≠ A86)

Sites not aligning to the query:

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
34% identity, 60% coverage: 79:204/209 of query aligns to 31:141/290 of 4mzuB

query
sites
4mzuB

I

I

G

G

N

R

H

N

C

C

M

N

I

I

A
x
C

A
|
A

D

N

C

S

F

L

L

I

H

E

G

N

P

D

I

V

T

V

L

I

G

G

N

D

E

N

V

V

A

T

I

I

N

K

H

S

G
|
G

C

V

S

Q

L

I

-

W

D

D

G

G

G

-

R

-

V

-

G

-

I

I

Q

H

I

I

G

Q

D

D

Q

D

T

V

R
x
F

I

I

A

G

N

P

H
x
N

V

V

T

T

I

-

Y

-

A

-

F

F

N
x
T

H
x
N

G
x
D

M
x
K

A

Q

P
|
P

D

-

T

-

P

-

I

-

Y

-

Q

-

A

-

S

-

H

R

S

S

K

L

G

K

I

T

V

I

I

V

G

K

K

K

D

G

V

A

W

S

I

I

G
|
G

A
|
A

Q

N

A

S

G

T

I

I

V

L

D

P

G

G

V

I

T

L

I

I

G

G

D

E

H

N

A

A

V

M

I

V

G
|
G

M
x
A

G

G

C

A

I

V

V

I

T
|
T

K
|
K

D

N

V

V

P

P

A

D

W

N

A

A

I

I

V

V

A
x
I

G

G

N

N

P

P

A

G

R
|
R

V

I

I

T

G

G

binding coenzyme a: F72 (≠ R123), T80 (≠ N134), N81 (≠ H135), D82 (≠ G136), K83 (≠ M137), P85 (= P139), G99 (= G162), A100 (= A163), G117 (= G180), A118 (≠ M181), T123 (= T186), K124 (= K187), I133 (≠ A196), R138 (= R201)
binding magnesium ion: G58 (= G106), N76 (≠ H127)
binding thymine: C38 (≠ A86), A39 (= A87)

Sites not aligning to the query:

binding coenzyme a: 199
binding thymine: 20, 21
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

4hurA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with acetyl coenzyme a (see paper)
33% identity, 57% coverage: 88:206/209 of query aligns to 47:166/211 of 4hurA

query
sites
4hurA

D

D

C

Q

F

V

L

L

H
x
Y

G

H

P

Y

I

E

T

V

L

I

G

G

N

D

E

K

V

L

A

I

I

I

N

G

H

R

G

F

C

C

S
|
S

L
x
I

D
x
G

G

P

G

G

R

T

V

T

G

F

I

I

Q

M

I

N

G

G

D
x
A

Q
x
N

T

H

R

R

I

M

-

D

A

G

N

S

H

T

V

Y

T

P

I

F

Y

H

A

L

F

F

N

R

H

M

G

G

M

W

A

E

P

K

D

Y

T

M

P

P

I

S

Y

L

Q

K

Q

D

A

L

S

P

H

L

S
x
K

K

G

G

D

I

I

V

E

I

I

G

G

K

N

D

D

V

V

W
|
W

I
|
I

G
|
G

A
x
R

Q

D

A

V

G

T

I

I

V
x
M

D

P

G

G

V

V

T

K

I

I

G

G

D

D

H

G

A

A

V

I

I

I

G
x
A

M
x
A

G

E

C

A

I

V

V

V

T
|
T

K

K

D

N

V

V

P

A

A

P

W

Y

A

S

I

I

V

V

A
x
G

G

G

N

N

P
|
P

A

L

R

K

V

F

I
|
I

G
x
R

D

K

R

R

binding acetyl coenzyme *a: Y51 (≠ H92), S67 (= S108), I68 (≠ L109), G69 (≠ D110), A79 (≠ D120), N80 (≠ Q121), K110 (≠ S150), W120 (= W160), I121 (= I161), G122 (= G162), R123 (≠ A163), M128 (≠ V168), A140 (≠ G180), A141 (≠ M181), T146 (= T186), G156 (≠ A196), P159 (= P199), I163 (= I203), R164 (≠ G204)

4husA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with virginiamycin m1 (see paper)
33% identity, 57% coverage: 88:206/209 of query aligns to 47:166/212 of 4husA

query
sites
4husA

D

D

C

Q

F

V

L

L

H

Y

G

H

P

Y

I

E

T

V

L

I

G

G

N

D

E

K

V

L

A

I

I

I

N

G

H

R

G

F

C

C

S

S

L

I

D

G

G

P

G

G

R

T

V

T

G

F

I

I

Q

M

I

N

G

G

D

A

Q

N

T

H

R

R

I

M

-

D

A

G

N

S

H

T

V

Y

T

P

I

F

Y

H

A

L

F

F

N

R

H

M

G

G

M

W

A

E

P

K

D

Y

T

M

P

P

I

S

Y

L

Q

K

Q

D

A

L

S

P

H

L

S

K

K

G

G

D

I

I

V

E

I

I

G

G

K

N

D

D

V

V

W

W

I

I

G

G

A

R

Q

D

A

V

G

T

I

I

V

M

D

P

G

G

V

V

T

K

I

I

G

G

D

D

H

G

A

A

V

I

I

I

G

A

M

A

G

E

C

A

I

V

V

V

T

T

K

K

D

N

V

V

P

A

A

P

W

Y

A

S

I

I

V

V

A

G

G

G

N

N

P

P

A

L

R

K

V

F

I

I

G

R

D

K

R

R

Sites not aligning to the query:

binding virginiamycin m1: 17, 36, 38

6x3cA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
33% identity, 57% coverage: 88:206/209 of query aligns to 47:166/207 of 6x3cA

query
sites
6x3cA

D

D

C

Q

F

V

L

L

H
x
Y

G

H

P
x
Y

I

E

T

V

L

I

G

G

N

D

E

K

V

L

A

I

I

I

N

G

H

R

G

F

C

C

S

S

L

I

D
x
G

G

P

G

G

R

T

V

T

G

F

I

I

Q

M

I

N

G
|
G

D

A

Q
x
N

T
x
H

R

R

I

M

-

D

A

G

N

S

H

T

V

Y

T

P

I

F

Y
x
H

A
x
L

F

F

N

R

H

M

G

G

M

W

A

E

P

K

D

Y

T
x
M

P
|
P

I

S

Y
x
L

Q

K

Q

D

A

L

S

P

H

L

S

K

K

G

G

D

I

I

V

E

I

I

G

G

K

N

D

D

V

V

W
|
W

I

I

G
|
G

A
x
R

Q

D

A

V

G

T

I

I

V
x
M

D

P

G

G

V

V

T

K

I

I

G

G

D

D

H

G

A

A

V

I

I

I

G
x
A

M
x
A

G

E

C

A

I

V

V

V

T

T

K

K

D

N

V

V

P

A

A

P

W

Y

A

S

I

I

V

V

A

G

G

G

N

N

P

P

A

L

R

K

V

F

I

I

G

R

D

K

R

R

binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: Y53 (≠ P94), G78 (= G119), N80 (≠ Q121), H81 (≠ T122), H91 (≠ Y131), L92 (≠ A132), M101 (≠ T141), P102 (= P142), L104 (≠ Y144)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: Y51 (≠ H92), G69 (≠ D110), N80 (≠ Q121), H81 (≠ T122), W120 (= W160), G122 (= G162), R123 (≠ A163), M128 (≠ V168), A140 (≠ G180), A141 (≠ M181)

Sites not aligning to the query:

binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: 17, 36

6x3jA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f0224 (46) (see paper)
33% identity, 57% coverage: 88:206/209 of query aligns to 47:166/206 of 6x3jA

query
sites
6x3jA

D

D

C

Q

F

V

L

L

H
x
Y

G

H

P
x
Y

I

E

T
x
V

L

I

G

G

N

D

E

K

V

L

A

I

I

I

N

G

H

R

G

F

C

C

S

S

L

I

D
x
G

G

P

G

G

R

T

V

T

G

F

I

I

Q

M

I

N

G

G

D
x
A

Q

N

T
x
H

R

R

I

M

-

D

A

G

N

S

H

T

V

Y

T

P

I

F

Y
x
H

A
x
L

F

F

N

R

H

M

G

G

M

W

A

E

P

K

D

Y

T
x
M

P
|
P

I

S

Y

L

Q

K

Q

D

A
x
L

S

P

H

L

S

K

K

G

G

D

I

I

V

E

I

I

G

G

K

N

D

D

V

V

W
|
W

I

I

G
|
G

A

R

Q

D

A

V

G

T

I

I

V

M

D

P

G

G

V

V

T

K

I

I

G

G

D

D

H

G

A

A

V

I

I

I

G

A

M

A

G

E

C

A

I

V

V

V

T

T

K

K

D

N

V

V

P

A

A

P

W

Y

A

S

I

I

V

V

A

G

G

G

N

N

P

P

A

L

R

K

V

F

I

I

G

R

D

K

R

R

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: Y51 (≠ H92), Y53 (≠ P94), V55 (≠ T96), H91 (≠ Y131), L92 (≠ A132), M101 (≠ T141), P102 (= P142), L107 (≠ A147)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (≠ D110), A79 (≠ D120), H81 (≠ T122), W120 (= W160), G122 (= G162)

6x3cE Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
33% identity, 57% coverage: 88:206/209 of query aligns to 47:166/203 of 6x3cE

query
sites
6x3cE

D

D

C

Q

F

V

L

L

H
x
Y

G

H

P
x
Y

I

E

T

V

L

I

G

G

N

D

E

K

V

L

A

I

I

I

N

G

H

R

G

F

C

C

S

S

L

I

D
x
G

G

P

G

G

R

T

V

T

G

F

I

I

Q

M

I

N

G
|
G

D

A

Q
x
N

T
x
H

R

R

I
x
M

-

D

A

G

N

S

H

T

V

Y

T

P

I

F

Y
x
H

A
x
L

F

F

N

R

H

M

G

G

M

W

A

E

P

K

D

Y

T
x
M

P
|
P

I

S

Y

L

Q

K

Q

D

A

L

S

P

H

L

S

K

K

G

G

D

I

I

V

E

I

I

G

G

K

N

D

D

V

V

W
|
W

I

I

G
|
G

A

R

Q

D

A

V

G

T

I

I

V

M

D

P

G

G

V

V

T

K

I

I

G

G

D

D

H

G

A

A

V

I

I

I

G
x
A

M
x
A

G

E

C

A

I

V

V

V

T

T

K

K

D

N

V

V

P

A

A

P

W

Y

A

S

I

I

V

V

A

G

G

G

N
|
N

P
|
P

A

L

R

K

V

F

I

I

G

R

D

K

R

R

binding magnesium ion: N158 (= N198), P159 (= P199)
binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: Y53 (≠ P94), G78 (= G119), N80 (≠ Q121), H81 (≠ T122), H91 (≠ Y131), L92 (≠ A132), M101 (≠ T141), P102 (= P142)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: Y51 (≠ H92), G69 (≠ D110), H81 (≠ T122), M83 (≠ I124), W120 (= W160), G122 (= G162), A140 (≠ G180), A141 (≠ M181)

6pubA Crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with crystal violet
31% identity, 68% coverage: 65:206/209 of query aligns to 15:165/210 of 6pubA

query
sites
6pubA

P

P

E

L

A

D

Q

Q

L

Q

F

V

A

T

E

N

P

P

N

N

R

-

D

-

I

I

N

I

I

V

G

G

N

K

H

H

C

S

M

Y

I

Y

A

S

A

G

-
x
Y

-

H

-

G

-

H

-

S

-

F

-

D

D

D

C

C

-

V

-

R

F

Y

L

L

H

H

G

P

P

E

I

R

T

D

L

D

G

V

N

D

E

K

V

L

A

V

I

I

N

G

H

S

G

F

C

C

S

S

L

I

D

G

G

S

G

G

R

A

V

V

G

F

I

M

Q

M

I

A

G

G

D

N

Q

Q

T

G

R

H

I

R

A

S

N

D

H

W

V

I

T

S

I

T

Y

F

A

P

F

F

N

F

H

Y

G

-

M

-

A

-

P

Q

D

D

T

N

P

D

I

N

Y

F

Q

A

Q

D

A

A

-

R

-

D

-

G

-

F

S

T

H

R

S

S

K

G

G

D

I

T

V

I

I

I

G

G

K

H

D

D

V

V

W

W

I

I

G

G

A

T

Q

E

A

A

G

M

I

I

V

M

D

P

G

G

V

V

T

K

I

I

G

G

D

H

H

G

A

A

V

I

I

I

G

A

M

S

G

R

C

S

I

V

V

V

T

T

K

K

D

D

V

V

P

A

A

P

W

Y

A

E

I

V

V

V

A

G

G

S

N

N

P

P

A

A

R

K

V

H

I

I

G

K

D

F

R

R

binding crystal violet: Y36 (vs. gap), Y37 (vs. gap)

Sites not aligning to the query:

binding crystal violet: 4, 7, 9, 10, 11

Query Sequence

>202262 FitnessBrowser__MR1:202262
MHTPITTALPDYQSQHKKRLSWMPWLYFSLKEKHLTWAKPWQNEVQTNLCALETVTIGDN
CFIAPEAQLFAEPNRDINIGNHCMIAADCFLHGPITLGNEVAINHGCSLDGGRVGIQIGD
QTRIANHVTIYAFNHGMAPDTPIYQQASHSKGIVIGKDVWIGAQAGIVDGVTIGDHAVIG
MGCIVTKDVPAWAIVAGNPARVIGDRRDK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory