SitesBLAST
Comparing 202522 FitnessBrowser__MR1:202522 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
O81852 Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
37% identity, 99% coverage: 3:812/822 of query aligns to 91:907/916 of O81852
- I441 (= I350) mutation to A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
- Q443 (= Q352) mutation to A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
- I522 (= I431) mutation to A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
- Q524 (= Q433) mutation to A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
2hmfA Structure of a threonine sensitive aspartokinase from methanococcus jannaschii complexed with mg-adp and aspartate (see paper)
37% identity, 56% coverage: 3:462/822 of query aligns to 3:463/464 of 2hmfA
- binding adenosine-5'-diphosphate: G7 (= G7), T229 (= T228), D230 (= D229), V231 (= V230), Y235 (= Y234), T237 (= T236), D238 (= D237), P239 (= P238), R240 (= R239), K265 (= K264), V266 (= V265)
- binding aspartic acid: S39 (= S38), T45 (= T44), F192 (= F191), R206 (= R205), G207 (≠ N206), S209 (= S208)
3c1nA Crystal structure of allosteric inhibition threonine-sensitive aspartokinase from methanococcus jannaschii with l-threonine (see paper)
37% identity, 56% coverage: 3:462/822 of query aligns to 3:458/458 of 3c1nA
- binding threonine: G7 (= G7), G8 (= G8), T9 (= T9), S10 (= S10), W227 (= W227), T228 (= T228), D229 (= D229), A406 (≠ H410), I409 (≠ V413), A410 (= A414), N423 (= N427), I424 (= I428), Q429 (= Q433), E433 (= E437)
3c1mC Cyrstal structure of threonine-sensitive aspartokinase from methanococcus jannaschii with mgamp-pnp and l-aspartate (see paper)
37% identity, 56% coverage: 3:462/822 of query aligns to 3:467/468 of 3c1mC
- binding phosphoaminophosphonic acid-adenylate ester: K5 (= K5), G7 (= G7), G8 (= G8), S39 (= S38), T229 (= T228), D230 (= D229), Y235 (= Y234), D238 (= D237), P239 (= P238), R240 (= R239), K265 (= K264), V266 (= V265)
- binding aspartic acid: T45 (= T44), E129 (= E128), F192 (= F191), R206 (= R205), G207 (≠ N206), S209 (= S208)
O94671 Homoserine dehydrogenase; HDH; HSD; EC 1.1.1.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
35% identity, 43% coverage: 467:818/822 of query aligns to 8:369/376 of O94671
- S201 (= S653) modified: Phosphoserine
2cdqA Crystal structure of arabidopsis thaliana aspartate kinase complexed with lysine and s- adenosylmethionine (see paper)
31% identity, 56% coverage: 3:462/822 of query aligns to 6:460/470 of 2cdqA
- binding lysine: S40 (= S38), A41 (= A39), T46 (= T44), E124 (= E128), M327 (= M326), Q330 (≠ M329), F333 (≠ M332), L334 (≠ A333), S347 (= S346), V348 (= V347), D349 (≠ S348)
- binding s-adenosylmethionine: G345 (= G344), I346 (≠ V345), S347 (= S346), W368 (≠ A369), S369 (≠ K370), R370 (≠ V371), L372 (= L373), E376 (= E378)
2j0xA Crystal structure of e. Coli aspartokinase iii in complex with lysine and aspartate (t-state) (see paper)
30% identity, 56% coverage: 3:462/822 of query aligns to 4:447/447 of 2j0xA
- binding aspartic acid: F182 (= F191), G197 (≠ N206), G198 (= G207), S199 (= S208), D200 (= D209)
- binding lysine: M316 (= M326), S319 (≠ M329), F322 (≠ M332), L323 (≠ A333), S336 (= S346), V337 (= V347), D338 (≠ S348), S343 (= S353), E344 (≠ S354)
2j0wA Crystal structure of e. Coli aspartokinase iii in complex with aspartate and adp (r-state) (see paper)
30% identity, 56% coverage: 3:462/822 of query aligns to 4:447/447 of 2j0wA
- binding adenosine-5'-diphosphate: T219 (= T228), D220 (= D229), I224 (≠ V233), Y225 (= Y234), D228 (= D237), R230 (= R239), K255 (= K264), V256 (= V265)
- binding aspartic acid: S37 (= S38), T43 (= T44), E117 (= E128), F182 (= F191), R196 (= R205), G197 (≠ N206), S199 (= S208)
P08660 Lysine-sensitive aspartokinase 3; Aspartate kinase III; AKIII; Lysine-sensitive aspartokinase III; EC 2.7.2.4 from Escherichia coli (strain K12) (see paper)
30% identity, 56% coverage: 3:462/822 of query aligns to 6:449/449 of P08660
- K8 (= K5) mutation to R: Reduces activity about 98%. Increases KM for aspartate about 40-fold, enzyme is less sensitive to lysine inhibition.
- E119 (= E128) mutation to D: Increases KM for aspartate about 3000-fold.
- R198 (= R205) mutation to K: Increases KM for aspartate about 200-fold.
- D202 (= D209) mutation to E: Reduces activity about 98%. Increases KM for aspartate about 40-fold, enzyme is less sensitive to lysine inhibition.
1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
32% identity, 39% coverage: 465:788/822 of query aligns to 3:331/358 of 1tveA