SitesBLAST
Comparing 202600 FitnessBrowser__MR1:202600 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q9A3Q9 Omega-aminotransferase; Beta-alanine--pyruvate aminotransferase; EC 2.6.1.-; EC 2.6.1.18 from Caulobacter vibrioides (strain ATCC 19089 / CIP 103742 / CB 15) (Caulobacter crescentus) (see paper)
58% identity, 95% coverage: 14:438/446 of query aligns to 8:431/439 of Q9A3Q9
- V227 (= V234) mutation to G: Decreases activity toward 3-aminobutanoate by 2-fold. Increases activity toward the aromatic beta-amino acid 3-amino-3-phenylpropanoate by 2-fold.
- R260 (= R267) mutation to L: Decreases activity toward 3-aminobutanoate by 30-fold.
- N285 (= N292) mutation to A: Decreases activity toward 3-aminobutanoate by 4-fold. Increases activity toward the aromatic beta-amino acid 3-amino-3-phenylpropanoate by 3-fold.
Q9I700 Beta-alanine--pyruvate aminotransferase; Beta-A--Py AT; Beta-alanine--pyruvate transaminase; Omega-amino acid aminotransferase; Omega-amino acid AT; Omega-amino acid--pyruvate aminotransferase; Omega-APT; EC 2.6.1.18 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
50% identity, 97% coverage: 14:445/446 of query aligns to 16:447/448 of Q9I700
- W61 (= W59) binding substrate
- T327 (= T325) binding pyridoxal 5'-phosphate
- R414 (= R412) binding substrate
- Q421 (≠ A419) binding substrate
4b98A The structure of the omega aminotransferase from pseudomonas aeruginosa (see paper)
50% identity, 97% coverage: 14:445/446 of query aligns to 9:440/441 of 4b98A
- active site: F17 (= F22), Y146 (= Y151), E219 (= E226), D252 (= D259), I255 (= I262), K281 (= K288), Q414 (≠ A419)
- binding pyridoxal-5'-phosphate: G233 (= G240), Q236 (≠ K243), F270 (≠ W277), G271 (= G278)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: L53 (= L58), W54 (= W59), F82 (= F87), S112 (= S117), G113 (= G118), S114 (= S119), Y146 (= Y151), H147 (= H152), G148 (= G153), E219 (= E226), S224 (= S231), D252 (= D259), V254 (= V261), I255 (= I262), K281 (= K288), Y319 (= Y324), T320 (= T325), R407 (= R412), Q414 (≠ A419)
4uhmA Characterization of a novel transaminase from pseudomonas sp. Strain aac (see paper)
50% identity, 97% coverage: 14:445/446 of query aligns to 3:434/435 of 4uhmA
- active site: F11 (= F22), Y140 (= Y151), E213 (= E226), D246 (= D259), I249 (= I262), K275 (= K288), Q408 (≠ A419)
- binding magnesium ion: A91 (≠ T102), D99 (≠ N110)
- binding pyridoxal-5'-phosphate: G107 (= G118), S108 (= S119), Y140 (= Y151), H141 (= H152), G142 (= G153), E213 (= E226), D246 (= D259), V248 (= V261), I249 (= I262), K275 (= K288)
3a8uX Crystal structure of omega-amino acid:pyruvate aminotransferase
49% identity, 97% coverage: 14:445/446 of query aligns to 8:440/441 of 3a8uX
- active site: Y145 (= Y151), D252 (= D259), K281 (= K288), Q414 (≠ A419)
- binding pyridoxal-5'-phosphate: S111 (= S117), G112 (= G118), S113 (= S119), Y145 (= Y151), H146 (= H152), G147 (= G153), E219 (= E226), D252 (= D259), V254 (= V261), I255 (= I262), K281 (= K288)
6gwiB The crystal structure of halomonas elongata amino-transferase (see paper)
38% identity, 94% coverage: 17:436/446 of query aligns to 13:436/450 of 6gwiB
- active site: F18 (= F22), Y149 (= Y151), D255 (= D259), K284 (= K288)
- binding pyridoxal-5'-phosphate: S115 (= S117), G116 (= G118), S117 (= S119), Y149 (= Y151), H150 (= H152), G151 (= G153), E222 (= E226), D255 (= D259), V257 (= V261), I258 (= I262), K284 (= K288)
7qx3A Structure of the transaminase tr2e2 with eos (see paper)
36% identity, 93% coverage: 33:446/446 of query aligns to 2:422/422 of 7qx3A
5lh9D Amine transaminase crystal structure from an uncultivated pseudomonas species in the plp-bound (internal aldimine) form
38% identity, 97% coverage: 16:446/446 of query aligns to 10:449/449 of 5lh9D
- active site: Y148 (= Y151), D255 (= D259), K284 (= K288), T321 (= T325)
- binding pyridoxal-5'-phosphate: G115 (= G118), S116 (= S119), Y148 (= Y151), H149 (= H152), G150 (= G153), E222 (= E226), D255 (= D259), V257 (= V261), K284 (= K288)
5lhaA Amine transaminase crystal structure from an uncultivated pseudomonas species in the pmp-bound form
38% identity, 97% coverage: 16:446/446 of query aligns to 8:447/447 of 5lhaA
- active site: Y146 (= Y151), D253 (= D259), K282 (= K288), T319 (= T325)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G113 (= G118), S114 (= S119), Y146 (= Y151), H147 (= H152), G148 (= G153), E220 (= E226), D253 (= D259), K282 (= K288), Y318 (= Y324), T319 (= T325)
6fyqA The crystal structure of a new transaminase from the marine bacterium virgibacillus (see paper)
34% identity, 96% coverage: 16:445/446 of query aligns to 9:438/443 of 6fyqA
- active site: F15 (= F22), Y147 (= Y151), D243 (= D259), K272 (= K288)
- binding pyridoxal-5'-phosphate: G114 (= G118), S115 (= S119), Y147 (= Y151), H148 (= H152), G149 (= G153), E210 (= E226), D243 (= D259), V245 (= V261), I246 (= I262), K272 (= K288)
6io1B Crystal structure of a novel thermostable (s)-enantioselective omega- transaminase from thermomicrobium roseum (see paper)
36% identity, 97% coverage: 14:445/446 of query aligns to 12:446/448 of 6io1B
- active site: L20 (≠ F22), Y151 (= Y151), D257 (= D259), K286 (= K288)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (≠ S117), G118 (= G118), A119 (≠ S119), N122 (≠ V122), Y151 (= Y151), H152 (= H152), D257 (= D259), V259 (= V261), I260 (= I262), K286 (= K288)
3i5tA Crystal structure of aminotransferase prk07036 from rhodobacter sphaeroides kd131
36% identity, 93% coverage: 29:445/446 of query aligns to 22:437/444 of 3i5tA
- active site: Y144 (= Y151), E216 (= E226), D249 (= D259), V252 (≠ I262), K279 (= K288), V411 (≠ A419)
- binding pyridoxal-5'-phosphate: G111 (= G118), S112 (= S119), Y144 (= Y151), H145 (= H152), E216 (= E226), D249 (= D259), V251 (= V261), K279 (= K288), Y315 (= Y324), T316 (= T325)
Sites not aligning to the query:
8wqjA Crystal structure of transaminase from shimia marina
34% identity, 96% coverage: 17:442/446 of query aligns to 19:447/472 of 8wqjA
- binding pyridoxal-5'-phosphate: S121 (= S117), G122 (= G118), S123 (= S119), Y155 (= Y151), H156 (= H152), G157 (= G153), E228 (= E226), D261 (= D259), V263 (= V261), I264 (= I262), K290 (= K288), Y321 (= Y324), T322 (= T325)
7qx0B Transaminase structure of plurienzyme (tr2e2) in complex with plp (see paper)
34% identity, 96% coverage: 17:446/446 of query aligns to 12:443/443 of 7qx0B
7ypmA Crystal structure of transaminase cc1012 complexed with plp and l- alanine (see paper)
37% identity, 92% coverage: 33:442/446 of query aligns to 31:444/454 of 7ypmA
- binding alanine: W57 (= W59), Y150 (= Y151)
- binding pyridoxal-5'-phosphate: S116 (= S117), G117 (= G118), S118 (= S119), Y150 (= Y151), G152 (= G153), E223 (= E226), D256 (= D259), V258 (= V261), I259 (= I262), K285 (= K288)
6s4gA Crystal structure of the omega transaminase from chromobacterium violaceum in complex with pmp (see paper)
35% identity, 94% coverage: 17:435/446 of query aligns to 12:434/453 of 6s4gA
- active site: F17 (= F22), Y148 (= Y151), D254 (= D259), K283 (= K288)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S114 (= S117), G115 (= G118), S116 (= S119), Y148 (= Y151), H149 (= H152), G150 (= G153), E221 (= E226), D254 (= D259), V256 (= V261), I257 (= I262), K283 (= K288), F315 (≠ Y324), T316 (= T325)
7q9xAAA Probable aminotransferase
35% identity, 94% coverage: 17:435/446 of query aligns to 13:435/455 of 7q9xAAA
- binding (3E)-4-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}-2-oxobut-3-enoic acid: L55 (= L58), W56 (= W59), S115 (= S117), G116 (= G118), S117 (= S119), Y149 (= Y151), G151 (= G153), E222 (= E226), D255 (= D259), V257 (= V261), I258 (= I262), K284 (= K288), F316 (≠ Y324), T317 (= T325), R412 (= R412)
- binding pyridoxal-5'-phosphate: F316 (≠ Y324), T317 (= T325)
4a6tC Crystal structure of the omega transaminase from chromobacterium violaceum in complex with plp (see paper)
35% identity, 94% coverage: 17:435/446 of query aligns to 13:435/455 of 4a6tC
- active site: F18 (= F22), Y149 (= Y151), E222 (= E226), D255 (= D259), I258 (= I262), K284 (= K288), V419 (≠ A419)
- binding pyridoxal-5'-phosphate: G116 (= G118), S117 (= S119), Y149 (= Y151), H150 (= H152), G151 (= G153), E222 (= E226), D255 (= D259), V257 (= V261), I258 (= I262), K284 (= K288)
6s54A Transaminase from pseudomonas fluorescens (see paper)
36% identity, 93% coverage: 32:444/446 of query aligns to 31:450/453 of 6s54A
- active site: Y152 (= Y151), D258 (= D259), K287 (= K288)
- binding pyridoxal-5'-phosphate: G119 (= G118), S120 (= S119), Y152 (= Y151), H153 (= H152), G154 (= G153), E225 (= E226), D258 (= D259), V260 (= V261), I261 (= I262), K287 (= K288)
Sites not aligning to the query:
4ba5A Crystal structure of omega-transaminase from chromobacterium violaceum (see paper)
35% identity, 90% coverage: 33:435/446 of query aligns to 2:407/427 of 4ba5A
- active site: Y121 (= Y151), E194 (= E226), D227 (= D259), I230 (= I262), K256 (= K288), V391 (≠ A419)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: L27 (= L58), W28 (= W59), G88 (= G118), S89 (= S119), Y121 (= Y151), H122 (= H152), G123 (= G153), E194 (= E226), A199 (≠ S231), D227 (= D259), V229 (= V261), R384 (= R412)
Query Sequence
>202600 FitnessBrowser__MR1:202600
MADSPNNLAHEHPSLEHYWMPFTANRQFKASPRLLAQAEGMYYTDINGNKVLDSTAGLWC
CNAGHGRREISEAVSKQIRQMDYAPSFQMGHPIAFELAERLTELSPEGLNKVFFTNSGSE
SVDTALKMALCYHRANGQASRTRFIGREMGYHGVGFGGISVGGLSNNRKAFSGQLLQGVD
HLPHTLDIQHAAFSRGLPSLGAEKAEVLEQLVTLHGAENIAAVIVEPMSGSAGVILPPQG
YLKRLREITKKHGILLIFDEVITAFGRVGAAFASQRWGVIPDIITTAKAINNGAIPMGAV
FVQDYIHDTCMQGPTELIEFFHGYTYSGHPVAAAAALATLSIYQNEQLFERSFELERYFE
EAVHSLKGLPNVIDIRNTGLVAGFQLAPNSQGVGKRGYSVFEHCFHQGTLVRATGDIIAM
SPPLIVEKHQIDQMVNSLSDAIHAVG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory