SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 202710 FitnessBrowser__MR1:202710 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P33221 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; GAR transformylase T; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Escherichia coli (strain K12) (see 5 papers)
69% identity, 99% coverage: 1:376/378 of query aligns to 16:390/392 of P33221

query
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P33221

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EL 22:23 (= EL 7:8) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
E82 (= E67) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
R114 (= R99) binding ATP
K155 (= K140) binding ATP
SSGKGQ 160:165 (= SSGKGQ 145:150) binding ATP
G162 (= G147) mutation to I: Strong decrease in the reaction rate for the conversion of formate to FGAR and in the affinity for formate. 3- and 2-fold decrease in the affinity for ATP and GAR, respectively.
K179 (≠ Q164) modified: N6-acetyllysine
EGVV 195:198 (≠ EGFI 180:183) binding ATP
E203 (= E188) binding ATP
E267 (= E252) binding Mg(2+)
E279 (= E264) binding Mg(2+)
D286 (= D271) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
K355 (= K341) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
RR 362:363 (= RR 348:349) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1kjiA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-amppcp (see paper)
69% identity, 99% coverage: 1:376/378 of query aligns to 15:387/389 of 1kjiA

query
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1kjiA

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active site: E114 (= E100), K154 (= K140), S159 (= S145), G161 (= G147), E264 (= E252), E276 (= E264), D283 (= D271), T284 (= T272), R360 (= R349)
binding phosphomethylphosphonic acid adenylate ester: R113 (= R99), I152 (≠ V138), K154 (= K140), S159 (= S145), S160 (= S146), G161 (= G147), Q164 (= Q150), E192 (= E180), V195 (≠ I183), E200 (= E188), Q222 (= Q210), E264 (= E252), F266 (= F254), E276 (= E264)
binding magnesium ion: E264 (= E252), E276 (= E264)

1ez1A Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg, amppnp, and gar (see paper)
69% identity, 99% coverage: 1:376/378 of query aligns to 15:387/389 of 1ez1A

query
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active site: E114 (= E100), K154 (= K140), S159 (= S145), G161 (= G147), E264 (= E252), E276 (= E264), D283 (= D271), T284 (= T272), R360 (= R349)
binding phosphoaminophosphonic acid-adenylate ester: R113 (= R99), I152 (≠ V138), K154 (= K140), S159 (= S145), S160 (= S146), G161 (= G147), E192 (= E180), V194 (≠ F182), V195 (≠ I183), F197 (= F185), E200 (= E188), Q222 (= Q210), E264 (= E252), F266 (= F254), E276 (= E264)
binding glycinamide ribonucleotide: G20 (= G6), E21 (= E7), L22 (= L8), E81 (= E67), I82 (= I68), S160 (= S146), D283 (= D271), K352 (= K341), R359 (= R348), R360 (= R349)
binding magnesium ion: E264 (= E252), E276 (= E264)

1eyzA Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg and amppnp (see paper)
69% identity, 99% coverage: 1:376/378 of query aligns to 15:387/389 of 1eyzA

query
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1eyzA

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D

A

A

P

P

A

A

M

M

Q

H

I

V

A

A

H

H

R

R

S

S

H

H

V

V

I

I

N

N

M

M

L

L

D

D

A

G

K

D

A

A

L

L

R

R

A

R

V

V

I

V

E

E

L

L

E

E

K

K

P

P

H

H

L

Y

V

I

I

V

P

P

E

E

I

I

E

E

A

A

I

I

A

A

T

T

Q

D

T

M

L

L

V

I

E

Q

M

L

E

E

A

E

E

E

G

G

L

L

N

N

V

V

P

P

T

C

A

A

R

R

A

A

T

T

K

K

L

L

T

T

M

M

D

N

R
|
R

E
|
E

G

G

I

I

R

R

L

L

A

A

E

E

T

E

L

L

G

Q

L

L

P

P

T

T

S

S

P

T

Y

Y

F

R

F

F

C

A

D

D

T

S

E

E

T

S

E

L

F

F

N

R

Q

E

A

A

I

V

S

A

K

D

I

I

G

G

V

Y

P

P

C

C

V
x
I

V

V

K
|
K

P

P

V

V

M

M

S

S

S
|
S

G
|
G

K

K

G

G

Q
|
Q

S

T

V

F

I

I

R

R

N

S

V

A

A

E

Q

Q

S

L

A

A

K

Q

A

A

W

W

Q

K

Y

Y

A

A

Q

Q

E

Q

G

G

R

R

A

A

G

-

G

G

R

R

V

V

I

I

V

V

E
|
E

G

G

F

V

I
x
V

P

K

F
|
F

D

D

Y

F

E
|
E

I

I

T

T

L

L

T

T

I

V

S

S

A

A

V

V

N

D

G

G

I

V

H

H

F

F

C

C

A

A

P

P

I

V

G

G

H

H

R

R

Q

Q

E

E

D

D

G

G

D

D

Y

Y

R

R

E

E

S

S

W

W

Q

Q

P

P

Q

Q

A

Q

M

M

S

S

A

P

D

L

V

A

L

L

A

E

K

R

S

A

Q

Q

A

E

I

I

A

A

S

R

K

K

V

V

E

L

A

A

L

L

G

G

Y

Y

G

G

L

L

F

F

G

G

V

V

E
|
E

L

L

F
|
F

V

V

K

C

G

G

S

D

D

E

V

V

Y

I

F

F

S

S

E
|
E

V

V

S

S

P

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

L

M

V

V

T

T

L

L

I

I

S

S

Q

Q

D

D

L

L

S

S

E

E

F

F

A

A

L

L

H

H

V

V

R

R

A

A

I

F

L

L

G

G

L

L

P

P

I

V

P

G

N

G

I

I

H

R

Q

Q

H

Y

G

G

P

P

S

A

A

A

S

S

A

A

V

V

V

I

L

L

V

P

E

Q

G

L

K

T

S

S

K

Q

N

N

I

V

R

T

Y

F

Q

D

G

N

L

V

A

Q

A

N

A

A

L

V

A

G

A

A

E

-

N

D

T

L

Q

Q

L

I

R

R

L

L

F

F

A

G

K

K

P

P

E

E

I

I

D

D

G

G

R

S

R

R

R
|
R

L

L

G

G

V

V

A

A

L

L

A

A

R

T

D

A

K

E

D

S

I

V

D

V

S

D

A

A

V

I

N

E

K

R

A

A

L

K

D

H

S

A

A

A

A

G

K

Q

V

V

Q

K

V

V

active site: E114 (= E100), K154 (= K140), S159 (= S145), G161 (= G147), E264 (= E252), E276 (= E264), D283 (= D271), T284 (= T272), R360 (= R349)
binding phosphoaminophosphonic acid-adenylate ester: R113 (= R99), I152 (≠ V138), K154 (= K140), S159 (= S145), S160 (= S146), G161 (= G147), Q164 (= Q150), E192 (= E180), V195 (≠ I183), F197 (= F185), E200 (= E188), E264 (= E252), F266 (= F254), E276 (= E264)
binding magnesium ion: E264 (= E252), E276 (= E264)

1kjqA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-adp (see paper)
69% identity, 99% coverage: 1:376/378 of query aligns to 15:386/388 of 1kjqA

query
sites
1kjqA

M

M

L

L

G

G

C

S

G

G

E

E

L

L

G

G

K

K

E

E

V

V

A

A

I

I

E

E

L

C

Q

Q

R

R

L

L

G

G

V

V

E

E

V

V

I

I

G

A

V

V

D

D

R

R

Y

Y

P

A

N

D

A

A

P

P

A

A

M

M

Q

H

I

V

A

A

H

H

R

R

S

S

H

H

V

V

I

I

N

N

M

M

L

L

D

D

A

G

K

D

A

A

L

L

R

R

A

R

V

V

I

V

E

E

L

L

E

E

K

K

P

P

H

H

L

Y

V

I

I

V

P

P

E

E

I

I

E

E

A

A

I

I

A

A

T

T

Q

D

T

M

L

L

V

I

E

Q

M

L

E

E

A

E

E

E

G

G

L

L

N

N

V

V

P

P

T

C

A

A

R

R

A

A

T

T

K

K

L

L

T

T

M

M

D

N

R
|
R

E
|
E

G

G

I

I

R

R

L

L

A

A

E

E

T

E

L

L

G

Q

L

L

P

P

T

T

S

S

P

T

Y

Y

F

R

F

F

C

A

D

D

T

S

E

E

T

S

E

L

F

F

N

R

Q

E

A

A

I

V

S

A

K

D

I

I

G

G

V

Y

P

P

C

C

V
x
I

V

V

K
|
K

P

P

V

V

M

M

S

S

S

-

G

-

K

K

G

G

Q

Q

S

T

V

F

I

I

R

R

N

S

V

A

A

E

Q

Q

S

L

A

A

K

Q

A

A

W

W

Q

K

Y

Y

A

A

Q

Q

E

Q

G

G

R

R

A

A

G

G

G

A

G

G

R

R

V

V

I

I

V

V

E
|
E

G

G

F
x
V

I
x
V

P

K

F
|
F

D

D

Y

F

E
|
E

I

I

T

T

L

L

T

T

I

V

S

S

A

A

V

V

N

D

G

G

I

V

H

H

F

F

C

C

A

A

P

P

I

V

G

G

H

H

R

R

Q
|
Q

E

E

D

D

G

G

D

D

Y

Y

R

R

E

E

S

S

W

W

Q

Q

P

P

Q

Q

A

Q

M

M

S

S

A

P

D

L

V

A

L

L

A

E

K

R

S

A

Q

Q

A

E

I

I

A

A

S

R

K

K

V

V

E

L

A

A

L

L

G

G

Y

Y

G

G

L

L

F

F

G

G

V

V

E
|
E

L

L

F
|
F

V

V

K

C

G

G

S

D

D

E

V

V

Y

I

F

F

S

S

E
|
E

V

V

S

S

P

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

L

M

V

V

T

T

L

L

I

I

S

S

Q

Q

D

D

L

L

S

S

E

E

F

F

A

A

L

L

H

H

V

V

R

R

A

A

I

F

L

L

G

G

L

L

P

P

I

V

P

G

N

G

I

I

H

R

Q

Q

H

Y

G

G

P

P

S

A

A

A

S

S

A

A

V

V

V

I

L

L

V

P

E

Q

G

L

K

T

S

S

K

Q

N

N

I

V

R

T

Y

F

Q

D

G

N

L

V

A

Q

A

N

A

A

L

V

A

G

A

A

E

-

N

D

T

L

Q

Q

L

I

R

R

L

L

F

F

A

G

K

K

P

P

E

E

I

I

D

D

G

G

R

S

R

R

R
|
R

L

L

G

G

V

V

A

A

L

L

A

A

R

T

D

A

K

E

D

S

I

V

D

V

S

D

A

A

V

I

N

E

K

R

A

A

L

K

D

H

S

A

A

A

A

G

K

Q

V

V

Q

K

V

V

active site: E114 (= E100), K154 (= K140), E263 (= E252), E275 (= E264), D282 (= D271), T283 (= T272), R359 (= R349)
binding adenosine-5'-diphosphate: R113 (= R99), I152 (≠ V138), K154 (= K140), E191 (= E180), V193 (≠ F182), V194 (≠ I183), F196 (= F185), E199 (= E188), Q221 (= Q210), F265 (= F254), E275 (= E264)
binding magnesium ion: E263 (= E252), E275 (= E264)

1kjjA Crystal structure of glycniamide ribonucleotide transformylase in complex with mg-atp-gamma-s (see paper)
68% identity, 99% coverage: 1:376/378 of query aligns to 15:384/386 of 1kjjA

query
sites
1kjjA

M

M

L

L

G

G

C

S

G

G

E

E

L

L

G

G

K

K

E

E

V

V

A

A

I

I

E

E

L

C

Q

Q

R

R

L

L

G

G

V

V

E

E

V

V

I

I

G

A

V

V

D

D

R

R

Y

Y

P

A

N

D

A

A

P

P

A

A

M

M

Q

H

I

V

A

A

H

H

R

R

S

S

H

H

V

V

I

I

N

N

M

M

L

L

D

D

A

G

K

D

A

A

L

L

R

R

A

R

V

V

I

V

E

E

L

L

E

E

K

K

P

P

H

H

L

Y

V

I

I

V

P

P

E

E

I

I

E

E

A

A

I

I

A

A

T

T

Q

D

T

M

L

L

V

I

E

Q

M

L

E

E

A

E

E

E

G

G

L

L

N

N

V

V

P

P

T

C

A

A

R

R

A

A

T

T

K

K

L

L

T

T

M

M

D

N

R
|
R

E
|
E

G

G

I

I

R

R

L

L

A

A

E

E

T

E

L

L

G

Q

L

L

P

P

T

T

S

S

P

T

Y

Y

F

R

F

F

C

A

D

D

T

S

E

E

T

S

E

L

F

F

N

R

Q

E

A

A

I

V

S

A

K

D

I

I

G

G

V

Y

P

P

C

C

V
x
I

V

V

K
|
K

P

P

V

V

M

M

S

S

S
|
S

G
|
G

K

K

G

G

Q
|
Q

S

T

V

F

I

I

R

R

N

S

V

A

A

E

Q

Q

S

L

A

A

K

Q

A

A

W

W

Q

K

Y

Y

A

A

Q

Q

E

Q

G

-

R

-

A

-

G

-

G

G

R

R

V

V

I

I

V

V

E
|
E

G

G

F

V

I
x
V

P

K

F

F

D

D

Y

F

E
|
E

I

I

T

T

L

L

T

T

I

V

S

S

A

A

V

V

N

D

G

G

I

V

H

H

F

F

C

C

A

A

P

P

I

V

G

G

H

H

R

R

Q
|
Q

E

E

D

D

G

G

D

D

Y

Y

R

R

E

E

S

S

W

W

Q

Q

P

P

Q

Q

A

Q

M

M

S

S

A

P

D

L

V

A

L

L

A

E

K

R

S

A

Q

Q

A

E

I

I

A

A

S

R

K

K

V

V

E

L

A

A

L

L

G

G

Y

Y

G

G

L

L

F

F

G

G

V

V

E
|
E

L

L

F
|
F

V

V

K

C

G

G

S

D

D

E

V

V

Y

I

F

F

S

S

E
|
E

V

V

S

S

P

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

L

M

V

V

T

T

L

L

I

I

S

S

Q

Q

D

D

L

L

S

S

E

E

F

F

A

A

L

L

H

H

V

V

R

R

A

A

I

F

L

L

G

G

L

L

P

P

I

V

P

G

N

G

I

I

H

R

Q

Q

H

Y

G

G

P

P

S

A

A

A

S

S

A

A

V

V

V

I

L

L

V

P

E

Q

G

L

K

T

S

S

K

Q

N

N

I

V

R

T

Y

F

Q

D

G

N

L

V

A

Q

A

N

A

A

L

V

A

G

A

A

E

-

N

D

T

L

Q

Q

L

I

R

R

L

L

F

F

A

G

K

K

P

P

E

E

I

I

D

D

G

G

R

S

R

R

R
|
R

L

L

G

G

V

V

A

A

L

L

A

A

R

T

D

A

K

E

D

S

I

V

D

V

S

D

A

A

V

I

N

E

K

R

A

A

L

K

D

H

S

A

A

A

A

G

K

Q

V

V

Q

K

V

V

active site: E114 (= E100), K154 (= K140), S159 (= S145), G161 (= G147), E261 (= E252), E273 (= E264), D280 (= D271), T281 (= T272), R357 (= R349)
binding phosphothiophosphoric acid-adenylate ester: R113 (= R99), I152 (≠ V138), K154 (= K140), S159 (= S145), S160 (= S146), G161 (= G147), Q164 (= Q150), E189 (= E180), V192 (≠ I183), E197 (= E188), Q219 (= Q210), E261 (= E252), F263 (= F254), E273 (= E264)
binding magnesium ion: E261 (= E252), E273 (= E264)

1kj8A Crystal structure of purt-encoded glycinamide ribonucleotide transformylase in complex with mg-atp and gar (see paper)
68% identity, 99% coverage: 1:376/378 of query aligns to 15:384/386 of 1kj8A

query
sites
1kj8A

M

M

L

L

G

G

C

S

G
|
G

E
|
E

L
|
L

G

G

K

K

E

E

V

V

A

A

I

I

E

E

L

C

Q

Q

R

R

L

L

G

G

V

V

E

E

V

V

I

I

G

A

V

V

D

D

R

R

Y

Y

P

A

N

D

A

A

P

P

A

A

M

M

Q

H

I

V

A

A

H

H

R

R

S

S

H

H

V

V

I

I

N

N

M

M

L

L

D

D

A

G

K

D

A

A

L

L

R

R

A

R

V

V

I

V

E

E

L

L

E

E

K

K

P

P

H

H

L

Y

V

I

I

V

P

P

E
|
E

I
|
I

E

E

A

A

I

I

A

A

T

T

Q

D

T

M

L

L

V

I

E

Q

M

L

E

E

A

E

E

E

G

G

L

L

N

N

V

V

P

P

T

C

A

A

R

R

A

A

T

T

K

K

L

L

T

T

M

M

D

N

R
|
R

E
|
E

G

G

I

I

R

R

L

L

A

A

E

E

T

E

L

L

G

Q

L

L

P

P

T

T

S

S

P

T

Y

Y

F

R

F

F

C

A

D

D

T

S

E

E

T

S

E

L

F

F

N

R

Q

E

A

A

I

V

S

A

K

D

I

I

G

G

V

Y

P

P

C

C

V
x
I

V

V

K
|
K

P

P

V

V

M

M

S

S

S
|
S

G
|
G

K

K

G

G

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active site: E114 (= E100), K154 (= K140), S159 (= S145), G161 (= G147), E261 (= E252), E273 (= E264), D280 (= D271), T281 (= T272), R357 (= R349)
binding adenosine-5'-triphosphate: R113 (= R99), I152 (≠ V138), K154 (= K140), S159 (= S145), S160 (= S146), G161 (= G147), Q164 (= Q150), E189 (= E180), V192 (≠ I183), F194 (= F185), E197 (= E188), Q219 (= Q210), G222 (= G213), E261 (= E252), F263 (= F254), E273 (= E264)
binding glycinamide ribonucleotide: G20 (= G6), E21 (= E7), L22 (= L8), E81 (= E67), I82 (= I68), S160 (= S146), D280 (= D271), K349 (= K341), R356 (= R348)
binding magnesium ion: E261 (= E252), E273 (= E264)

O58056 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
49% identity, 97% coverage: 1:367/378 of query aligns to 20:401/430 of O58056

query
sites
O58056

M

L

L

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R118 (= R99) binding ATP
K159 (= K140) binding ATP
EEHI 199:202 (≠ EGFI 180:183) binding ATP
E207 (= E188) binding ATP

2dwcB Crystal structure of probable phosphoribosylglycinamide formyl transferase from pyrococcus horikoshii ot3 complexed with adp
47% identity, 97% coverage: 1:367/378 of query aligns to 22:390/409 of 2dwcB

query
sites
2dwcB

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E

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A

active site: E265 (= E252), E277 (= E264), D284 (= D271), T285 (= T272), R372 (= R349)
binding adenosine-5'-diphosphate: R120 (= R99), H159 (≠ V138), K161 (= K140), H190 (≠ F182), I191 (= I183), F193 (= F185), E196 (= E188), F267 (= F254), E277 (= E264)

3aw8A Crystal structure of n5-carboxyaminoimidazole ribonucleotide synthetase from thermus thermophilus hb8
29% identity, 90% coverage: 19:357/378 of query aligns to 21:338/360 of 3aw8A

query
sites
3aw8A

L

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H

D
x
N

T
x
S

G

G

L

H

V

W

T

T

L

I

I

E

S

G

Q

A

D

E

L

T

S

S

E

Q

F

F

A

E

L

N

H

H

V

L

R

R

A

A

I

V

L

L

G

G

L

L

P

P

I

L

P

G

N

S

I

T

H

A

Q

P

H

R

G

G

P

Q

S

S

A

A

S

M

A

V

V

N

V

L

L

I

V

G

E

E

G

K

K

P

S

P

K

-

N

-

I

-

R

-

Y

-

Q

-

G

-

L

F

A

A

A

E

A

V

L

L

A

K

A

V

E

E

N

G

T

A

Q

H

L

L

R

H

L

W

F

Y

A

G

K

K

P

A

E

V

I

R

D

P

G

G

R

R

R
x
K

R

V

L

G

G

H

V

I

A

T

L

L

A

R

R

R

D

D

active site: E240 (= E252), E252 (= E264), N259 (≠ D271), S260 (≠ T272), K329 (≠ R348)
binding adenosine monophosphate: L135 (≠ V138), K137 (= K140), Q142 (= Q150), F168 (= F182), V169 (≠ I183), E174 (= E188), H197 (≠ Q210), F242 (= F254), E252 (= E264)

3ax6A Crystal structure of n5-carboxyaminoimidazole ribonucleotide synthetase from thermotoga maritima
26% identity, 97% coverage: 12:377/378 of query aligns to 16:358/360 of 3ax6A

query
sites
3ax6A

V

M

A

T

I

L

E

E

L

A

Q

K

R

K

L

M

G

G

V

F

E

Y

V

V

I

I

G

V

V

L

D

D

R

P

Y

T

P

P

N

R

A

S

P

P

A

A

M

G

Q

Q

I

V

A

A

H

D

R

E

S

Q

H

I

V

V

I

A

N

G

M

F

L

F

D

D

A

S

K

E

A

R

L

I

R

E

A

D

V

L

I

V

E

K

L

-

E

-

K

G

P

S

H

D

L

V

V

T

I

T

P

Y

E

D

I

L

E

E

A

H

I

I

A

D

T

V

Q

Q

T

T

L

L

V

K

E

K

M

L

E

Y

A

N

E

E

G

G

L

Y

N

K

V

I

V

H

P

P

T

S

A

P

R

Y

A

T

T

L

K

E

L

I

T

I

M

Q

D

D

R
x
K

E

-

G

F

I

V

R

Q

R

K

L

E

A

F

A

L

E

K

T

K

L

N

G

G

L

I

P

P

T

V

S

P

P

E

Y

Y

F

K

F

L

C

V

-

K

D

D

T

L

E

E

T

S

E

D

F

-

N

-

Q

-

A

-

I

V

S

R

K

E

I

F

G

G

V

F

P

P

C

V

V
|
V

V

Q

K
|
K

P

-

V

-

M

-

S

-

S

A

S

R

G

K

K

G

G

G

Q

V

S

F

V

I

I

I

R

K

N

N

V

E

A

K

Q

D

S

L

A

E

K

N

A

A

W

I

Q

K

Y

-

A

-

Q

-

E

-

G

-

R

-

A

-

G

-

G

G

R

E

V

T

I

Y

V

L

E
|
E

G

E

F
|
F

I
x
V

P

E

F

I

D

E

Y

K

E
|
E

I

L

T

A

L

V

L

M

T

V

I

A

S

R

A

N

V

E

N

K

G

G

I

E

H

I

F

A

C

C

A

Y

P

P

I

V

G

V

H

E

R

M

Q

-

E

-

D

-

G

-

D

-

Y

Y

R

D

E

T

S

V

W

I

Q

A

P

P

Q

A

A

R

M

I

S

E

A

E

D

K

V

Y

L

S

A

K

K

I

S

A

Q

R

A

E

I

I

A

A

S

T

K

S

V

V

E

E

A

A

L

L

G

E

G

G

Y

V

G

G

L

I

F

F

G

G

V

I

E
|
E

L

M

F
|
F

V

L

-

T

K

K

G

Q

S

G

D

E

V

I

Y

L

F

V

S
x
N

E
|
E

V

I

S

A

P

P

R

R

P

P

H

H

D
x
N

T
x
S

G

G

L

H

V

Y

T

T

L

I

I

E

S

A

Q

C

D

V

L

T

S

S

E

Q

F

F

A

E

L

Q

H

H

V

I

R

R

A

A

I

I

L

M

G

N

L

L

P

P

I

L

P

G

N

S

I

T

H

E

Q

L

H

L

G

I

P

P

S

A

A

V

S

M

A

V

V

N

V

L

L

L

V

G

E

E

-

E

-

G

-

Y

G

G

K

K

S

P

K

A

N

L

I

I

R

-

Y

-

Q

-

G

G

L

L

A

E

A

A

L

L

A

A

A

I

E

E

N

G

T

L

Q

S

L

L

R

H

L

F

F

Y

A

G

K

K

P

K

E

E

I

T

D

R

G

P

R

Y

R

R

R
x
K

L

M

G

G

V

H

A

F

L

T

A

V

R

V

D

D

K

R

D

D

I

V

D

E

S

R

A

A

V

L

N

E

K

K

A

A

L

L

D

R

S

A

A

K

K

I

V

L

Q

K

V

V

I

V

active site: E231 (= E252), E244 (= E264), N251 (≠ D271), S252 (≠ T272), K330 (≠ R349)
binding adenosine-5'-diphosphate: K101 (≠ R99), V136 (= V138), K138 (= K140), E164 (= E180), F166 (= F182), V167 (≠ I183), E172 (= E188), F233 (= F254), N243 (≠ S263)

3r5hA Crystal structure of adp-air complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
26% identity, 96% coverage: 7:367/378 of query aligns to 18:366/383 of 3r5hA

query
sites
3r5hA

E
x
Q

L
|
L

G

G

K

R

E

M

V

M

A

A

I

L

E

A

L

A

Q

K

R

E

L

M

G

G

V

Y

E

K

V

I

I

A

G

V

V

L

D

D

R

P

Y

T

P

K

N

N

A

S

P

P

A

C

M

A

Q

Q

I

V

A

A

H

D

R

I

S

E

H

I

V

V

I

A

N

S

M

Y

L

D

D

D

A

L

K

K

A

A

L

I

R

Q

A

H

V

L

I

A

E

E

L

I

E

S

K

D

P

-

H

-

L

V

V

V

I

T

P

Y

E
|
E

I

F

E

E

A

N

I

I

A

D

T

Y

Q

R

T

C

L

L

V

Q

E

W

M

L

E

E

A

K

E

H

G

A

L

Y

N

-

V

-

V

L

P

P

T

Q

A

G

R

S

A

Q

T

L

K

L

L

S

T

K

M

T

D

Q

R

N

E
x
R

G

F

I

T

R

E

R

K

L

N

A

A

A

I

E

E

T

K

L

A

G

G

L

L

P

P

T

V

S

A

P

T

Y

Y

F

R

F

L

C

V

D

Q

T

N

E

Q

T

E

E

Q

F

L

N

T

Q

E

A

A

I

I

S

A

K

E

I

L

G

S

V

Y

P

P

C

S

V

V

V

L

K
|
K

P

T

V

T

M

T

S

G

-
x
Y

S

D

G

G

K

K

G

G

Q
|
Q

S

V

V

V

I

L

R

R

N

S

V

E

A

A

Q

D

S

V

A

D

K

E

A

A

W

R

Q

K

Y

L

A

A

Q

-

E

-

G

-

R

-

A

-

G

N

G

A

R

E

V

C

I

I

V

L

E

E

G

K

F
x
W

I
x
V

P

P

F
|
F

D

E

Y

K

E
|
E

I

V

T

S

L

V

L

I

T

V

I

I

S

R

A

S

V

V

N

S

G

G

I

E

H

T

F

K

C

V

A

F

P

P

I

V

G

A

H

E

R

N

-

I

Q

H

E

V

D

N

G
x
N

D

I

Y

L

R

H

E

E

S

S

W

I

Q

V

P

P

Q

A

A

R

M

I

S

T

A

E

D

E

V

L

L

S

A

Q

K

K

S

A

Q

I

A

A

I

Y

A

A

S

K

K

V

V

L

V

A

E

D

A

E

L

L

G

E

G

L

Y

V

G

G

L

T

F

L

G

A

V

V

E

E

L

M

F
|
F

V

A

K

T

G

A

S

D

-

G

D

E

V

I

Y

Y

F

I

S
x
N

E
|
E

V

L

S

A

P

P

R
|
R

P

P

H

H

D

N

T

S

G

G

L

H

V

Y

T

T

L

Q

I

D

S

A

Q

C

D

E

L

T

S

S

E

Q

F

F

A

G

L

Q

H

H

V

I

R

R

A

A

I

I

L

C

G

N

L

L

P

P

I

L

P

G

N

E

I

T

H

N

Q

L

H

L

G

K

P

P

S

-

A

-

S

-

A

-

V

V

L

M

V

V

E

-

G

-

K

-

S

-

K

-

N

N

I

I

R

L

Y

G

Q

E

G

H

L

I

A

E

A

G

A

V

L

L

A

R

A

Q

E

V

N

N

-

R

-

L

-

T

-

G

T

C

Q

Y

L

L

R

H

L

L

F

Y

A

G

K
|
K

P

E

E

E

I

A

D

K

G

A

R

Q

R
|
R

R

K

L

M

G

G

V

H

A

V

L

N

A

I

R

L

D

N

K

D

D

N

I

I

D

E

S

V

A

A

V

L

N

E

K

K

A

A

binding adenosine-5'-diphosphate: R107 (≠ E100), K147 (= K140), Q158 (= Q150), W184 (≠ F182), V185 (≠ I183), F187 (= F185), E190 (= E188), N216 (≠ G213), F257 (= F254), N267 (≠ S263), E268 (= E264)
binding 5-aminoimidazole ribonucleotide: Q18 (≠ E7), L19 (= L8), E76 (= E67), Y153 (vs. gap), R272 (= R268), K340 (= K341), R347 (= R348)

Sites not aligning to the query:

binding 5-aminoimidazole ribonucleotide: 17

3q2oB Crystal structure of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
26% identity, 96% coverage: 7:367/378 of query aligns to 18:366/377 of 3q2oB

query
sites
3q2oB

E

Q

L

L

G

G

K

R

E

M

V

M

A

A

I

L

E

A

L

A

Q

K

R

E

L

M

G

G

V

Y

E

K

V

I

I

A

G

V

V

L

D

D

R

P

Y

T

P

K

N

N

A

S

P

P

A

C

M

A

Q

Q

I

V

A

A

H

D

R

I

S

E

H

I

V

V

I

A

N

S

M

Y

L

D

D

D

A

L

K

K

A

A

L

I

R

Q

A

H

V

L

I

A

E

E

L

I

E

S

K

D

P

-

H

-

L

V

V

V

I

T

P

Y

E

E

I

F

E

E

A

N

I

I

A

D

T

Y

Q

R

T

C

L

L

V

Q

E

W

M

L

E

E

A

K

E

H

G

A

L

Y

N

-

V

-

V

L

P

P

T

Q

A

G

R

S

A

Q

T

L

K

L

L

S

T

K

M
x
T

D

Q

R

N

E

R

G

F

I

T

R
x
E

R

K

L

N

A

A

A

I

E

E

T

K

L

A

G

G

L

L

P

P

T

V

S

A

P

T

Y

Y

F

R

F

L

C

V

D

Q

T

N

E

Q

T

E

E

Q

F

L

N

T

Q

E

A

A

I

I

S

A

K

E

I

L

G

S

V

Y

P

P

C

S

V

V

V

L

K

K

P

T

V

T

M

T

S

G

-

Y

S

D

G

G

K

K

G

G

Q

Q

S

V

V

V

I

L

R

R

N

S

V

E

A

A

Q

D

S

V

A

D

K

E

A

A

W

R

Q

K

Y

L

A

A

Q

-

E

-

G

-

R

-

A

-

G

N

G

A

R

E

V

C

I

I

V

L

E

E

G

K

F

W

I

V

P

P

F

F

D

E

Y

K

E

E

I

V

T

S

L

V

L

I

T

V

I

I

S

R

A

S

V

V

N

S

G

G

I

E

H

T

F

K

C

V

A

F

P

P

I

V

G

A

H

E

R

N

-

I

Q

H

E

V

D

N

G

N

D

I

Y

L

R

H

E

E

S

S

W

I

Q

V

P

P

Q

A

A

R

M

I

S

T

A

E

D

E

V

L

L

S

A

Q

K

K

S

A

Q

I

A

A

I

Y

A

A

S

K

K

V

V

L

V

A

E

D

A

E

L

L

G

E

G

L

Y

V

G

G

L

T

F

L

G

A

V

V

E

E

L

M

F

F

V

A

K

T

G

A

S

D

-

G

D

E

V

I

Y

Y

F

I

S

N

E

E

V
x
L

S

A

P

P

R

R

P

P

H

H

D

N

T

S

G

G

L

H

V

Y

T

T

L

Q

I

D

S

A

Q

C

D

E

L

T

S

S

E

Q

F

F

A

G

L

Q

H

H

V

I

R

R

A

A

I

I

L

C

G

N

L

L

P

P

I

L

P

G

N

E

I

T

H

N

Q

L

H

L

G

K

P

P

S

-

A

-

S

-

A

-

V

V

L

M

V

V

E

-

G

-

K

-

S

-

K

-

N

N

I

I

R

L

Y

G

Q

E

G

H

L

I

A

E

A

G

A

V

L

L

A

R

A

Q

E

V

N

N

-

R

-

L

-

T

-

G

T

C

Q

Y

L

L

R

H

L

L

F

Y

A

G

K

K

P

E

E

E

I

A

D

K

G

A

R

Q

R

R

R

K

L

M

G

G

V

H

A

V

L

N

A

I

R

L

D

N

K

D

D

N

I

I

D

E

S

V

A

A

V

L

N

E

K

K

A

A

binding magnesium ion: T104 (≠ M97), E110 (≠ R103), L269 (≠ V265)

3v4sB Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
26% identity, 96% coverage: 7:367/378 of query aligns to 19:367/381 of 3v4sB

query
sites
3v4sB

E

Q

L

L

G

G

K

R

E

M

V

M

A

A

I

L

E

A

L

A

Q

K

R

E

L

M

G

G

V

Y

E

K

V

I

I

A

G

V

V

L

D

D

R

P

Y

T

P

K

N

N

A

S

P

P

A

C

M

A

Q

Q

I

V

A

A

H

D

R

I

S

E

H

I

V

V

I

A

N

S

M

Y

L

D

D

D

A

L

K

K

A

A

L

I

R

Q

A

H

V

L

I

A

E

E

L

I

E

S

K

D

P

-

H

-

L

V

V

V

I

T

P

Y

E

E

I

F

E

E

A

N

I

I

A

D

T

Y

Q

R

T

C

L

L

V

Q

E

W

M

L

E

E

A

K

E

H

G

A

L

Y

N

-

V

-

V

L

P

P

T

Q

A

G

R

S

A

Q

T

L

K

L

L

S

T

K

M
x
T

D

Q

R

N

E
x
R

G

F

I

T

R
x
E

R

K

L

N

A

A

A

I

E

E

T

K

L

A

G

G

L

L

P

P

T

V

S

A

P

T

Y

Y

F

R

F

L

C

V

D

Q

T

N

E

Q

T

E

E

Q

F

L

N

T

Q

E

A

A

I

I

S

A

K

E

I

L

G

S

V

Y

P

P

C

S

V

V

V

L

K
|
K

P

T

V

T

M

T

S

G

-
x
Y

S
x
D

G
|
G

K

K

G

G

Q
|
Q

S

V

V

V

I

L

R

R

N

S

V

E

A

A

Q

D

S

V

A

D

K

E

A

A

W

R

Q

K

Y

L

A

A

Q

-

E

-

G

-

R

-

A

-

G

N

G

A

R

E

V

C

I

I

V

L

E
|
E

G

K

F
x
W

I
x
V

P

P

F
|
F

D

E

Y

K

E
|
E

I

V

T

S

L

V

L

I

T

V

I

I

S

R

A

S

V

V

N

S

G

G

I

E

H

T

F

K

C

V

A

F

P

P

I

V

G

A

H

E

R

N

-

I

Q
x
H

E

V

D

N

G
x
N

D

I

Y

L

R

H

E

E

S

S

W

I

Q

V

P

P

Q

A

A

R

M

I

S

T

A

E

D

E

V

L

L

S

A

Q

K

K

S

A

Q

I

A

A

I

Y

A

A

S

K

K

V

V

L

V

A

E

D

A

E

L

L

G

E

G

L

Y

V

G

G

L

T

F

L

G

A

V

V

E
|
E

L

M

F
|
F

V

A

K

T

G

A

S

D

-

G

D

E

V

I

Y

Y

F

I

S

N

E
|
E

V
x
L

S

A

P

P

R
|
R

P

P

H
|
H

D
x
N

T

S

G

G

L

H

V

Y

T

T

L

Q

I

D

S

A

Q

C

D

E

L

T

S

S

E

Q

F

F

A

G

L

Q

H

H

V

I

R

R

A

A

I

I

L

C

G

N

L

L

P

P

I

L

P

G

N

E

I

T

H

N

Q

L

H

L

G

K

P

P

S

-

A

-

S

-

A

-

V

V

L

M

V

V

E

-

G

-

K

-

S

-

K

-

N

N

I

I

R

L

Y

G

Q

E

G

H

L

I

A

E

A

G

A

V

L

L

A

R

A

Q

E

V

N

N

-

R

-

L

-

T

-

G

T

C

Q

Y

L

L

R

H

L

L

F

Y

A

G

K

K

P

E

E

E

I

A

D

K

G

A

R

Q

R

R

R

K

L

M

G

G

V

H

A

V

L

N

A

I

R

L

D

N

K

D

D

N

I

I

D

E

S

V

A

A

V

L

N

E

K

K

A

A

binding adenosine-5'-triphosphate: R108 (≠ E100), K148 (= K140), Y154 (vs. gap), D155 (≠ S146), G156 (= G147), Q159 (= Q150), E183 (= E180), W185 (≠ F182), V186 (≠ I183), F188 (= F185), E191 (= E188), H214 (≠ Q210), N217 (≠ G213), E256 (= E252), F258 (= F254), E269 (= E264)
binding carbonate ion: R273 (= R268), H275 (= H270), N276 (≠ D271)
binding magnesium ion: T105 (≠ M97), E111 (≠ R103), E256 (= E252), E269 (= E264), L270 (≠ V265)

3v4sA Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
26% identity, 96% coverage: 7:367/378 of query aligns to 17:365/380 of 3v4sA

query
sites
3v4sA

E

Q

L

L

G

G

K

R

E

M

V

M

A

A

I

L

E

A

L

A

Q

K

R

E

L

M

G

G

V

Y

E

K

V

I

I

A

G

V

V

L

D

D

R

P

Y

T

P

K

N

N

A

S

P

P

A

C

M

A

Q

Q

I

V

A

A

H

D

R

I

S

E

H

I

V

V

I

A

N

S

M

Y

L

D

D

D

A

L

K

K

A

A

L

I

R

Q

A

H

V

L

I

A

E

E

L

I

E

S

K

D

P

-

H

-

L

V

V

V

I

T

P

Y

E

E

I

F

E

E

A

N

I

I

A

D

T

Y

Q

R

T

C

L

L

V

Q

E

W

M

L

E

E

A

K

E

H

G

A

L

Y

N

-

V

-

V

L

P

P

T

Q

A

G

R

S

A

Q

T

L

K

L

L

S

T

K

M

T

D

Q

R

N

E
x
R

G

F

I

T

R

E

R

K

L

N

A

A

A

I

E

E

T

K

L

A

G

G

L

L

P

P

T

V

S

A

P

T

Y

Y

F

R

F

L

C

V

D

Q

T

N

E

Q

T

E

E

Q

F

L

N

T

Q

E

A

A

I

I

S

A

K

E

I

L

G

S

V

Y

P

P

C

S

V

V

V

L

K
|
K

P

T

V

T

M

T

S

G

-
x
Y

S

D

G
|
G

K

K

G

G

Q
|
Q

S

V

V

V

I

L

R

R

N

S

V

E

A

A

Q

D

S

V

A

D

K

E

A

A

W

R

Q

K

Y

L

A

A

Q

-

E

-

G

-

R

-

A

-

G

N

G

A

R

E

V

C

I

I

V

L

E

E

G

K

F
x
W

I
x
V

P

P

F

F

D

E

Y

K

E
|
E

I

V

T

S

L

V

L

I

T

V

I

I

S

R

A

S

V

V

N

S

G

G

I

E

H

T

F

K

C

V

A

F

P

P

I

V

G

A

H

E

R

N

-

I

Q

H

E

V

D

N

G
x
N

D

I

Y

L

R

H

E

E

S

S

W

I

Q

V

P

P

Q

A

A

R

M

I

S

T

A

E

D

E

V

L

L

S

A

Q

K

K

S

A

Q

I

A

A

I

Y

A

A

S

K

K

V

V

L

V

A

E

D

A

E

L

L

G

E

G

L

Y

V

G

G

L

T

F

L

G

A

V

V

E

E

L

M

F
|
F

V

A

K

T

G

A

S

D

-

G

D

E

V

I

Y

Y

F

I

S
x
N

E
|
E

V

L

S

A

P

P

R
|
R

P

P

H
|
H

D
x
N

T

S

G

G

L

H

V

Y

T

T

L

Q

I

D

S

A

Q

C

D

E

L

T

S

S

E

Q

F

F

A

G

L

Q

H

H

V

I

R

R

A

A

I

I

L

C

G

N

L

L

P

P

I

L

P

G

N

E

I

T

H

N

Q

L

H

L

G

K

P

P

S

-

A

-

S

-

A

-

V

V

L

M

V

V

E

-

G

-

K

-

S

-

K

-

N

N

I

I

R

L

Y

G

Q

E

G

H

L

I

A

E

A

G

A

V

L

L

A

R

A

Q

E

V

N

N

-

R

-

L

-

T

-

G

T

C

Q

Y

L

L

R

H

L

L

F

Y

A

G

K

K

P

E

E

E

I

A

D

K

G

A

R

Q

R

R

R

K

L

M

G

G

V

H

A

V

L

N

A

I

R

L

D

N

K

D

D

N

I

I

D

E

S

V

A

A

V

L

N

E

K

K

A

A

binding adenosine-5'-diphosphate: R106 (≠ E100), K146 (= K140), Y152 (vs. gap), G154 (= G147), Q157 (= Q150), W183 (≠ F182), V184 (≠ I183), E189 (= E188), N215 (≠ G213), F256 (= F254), N266 (≠ S263), E267 (= E264)
binding carbonate ion: R271 (= R268), H273 (= H270), N274 (≠ D271)

4dlkA Crystal structure of atp-ca++ complex of purk: n5- carboxyaminoimidazole ribonucleotide synthetase (see paper)
26% identity, 96% coverage: 7:367/378 of query aligns to 18:366/380 of 4dlkA

query
sites
4dlkA

E

Q

L

L

G

G

K

R

E

M

V

M

A

A

I

L

E

A

L

A

Q

K

R

E

L

M

G

G

V

Y

E

K

V

I

I

A

G

V

V

L

D

D

R

P

Y

T

P

K

N

N

A

S

P

P

A

C

M

A

Q
|
Q

I

V

A
|
A

H

D

R

I

S

E

H

I

V

V

I

A

N

S

M

Y

L

D

D

D

A

L

K

K

A

A

L

I

R

Q

A

H

V

L

I

A

E

E

L

I

E

S

K

D

P

-

H

-

L

V

V

V

I

T

P

Y

E
|
E

I
x
F

E

E

A

N

I

I

A

D

T

Y

Q

R

T

C

L

L

V

Q

E

W

M

L

E

E

A

K

E

H

G

A

L

Y

N

-

V

-

V

L

P

P

T

Q

A

G

R

S

A

Q

T

L

K

L

L

S

T

K

M

T

D

Q

R

N

E
x
R

G

F

I

T

R

E

R

K

L

N

A

A

A

I

E

E

T

K

L

A

G

G

L

L

P

P

T

V

S

A

P

T

Y

Y

F

R

F

L

C

V

D

Q

T

N

E

Q

T

E

E

Q

F

L

N

T

Q

E

A

A

I

I

S

A

K

E

I

L

G

S

V

Y

P

P

C

S

V

V

V

L

K
|
K

P

T

V

T

M

T

S

G

-
x
Y

S
x
D

G
|
G

K

K

G

G

Q
|
Q

S

V

V

V

I

L

R

R

N

S

V

E

A

A

Q

D

S

V

A

D

K

E

A

A

W

R

Q

K

Y

L

A

A

Q

-

E

-

G

-

R

-

A

-

G

N

G

A

R

E

V

C

I

I

V

L

E

E

G

K

F
x
W

I
x
V

P

P

F
|
F

D

E

Y

K

E
|
E

I

V

T

S

L

V

L

I

T

V

I

I

S

R

A

S

V

V

N

S

G

G

I

E

H

T

F

K

C

V

A

F

P

P

I

V

G

A

H

E

R

N

-

I

Q

H

E

V

D

N

G

N

D

I

Y

L

R

H

E

E

S

S

W

I

Q

V

P

P

Q

A

A

R

M

I

S

T

A

E

D

E

V

L

L

S

A

Q

K

K

S

A

Q

I

A

A

I

Y

A

A

S

K

K

V

V

L

V

A

E

D

A

E

L

L

G

E

G

L

Y

V

G

G

L

T

F

L

G

A

V

V

E
|
E

L

M

F
|
F

V

A

K

T

G

A

S

D

-

G

D

E

V

I

Y

Y

F

I

S
x
N

E
|
E

V

L

S

A

P

P

R
|
R

P

P

H
|
H

D
x
N

T
x
S

G

G

L

H

V

Y

T

T

L

Q

I

D

S

A

Q

C

D

E

L

T

S

S

E

Q

F

F

A

G

L

Q

H

H

V

I

R

R

A

A

I

I

L

C

G

N

L

L

P

P

I

L

P

G

N

E

I

T

H

N

Q

L

H

L

G

K

P

P

S

-

A

-

S

-

A

-

V

V

L

M

V

V

E

-

G

-

K

-

S

-

K

-

N

N

I

I

R

L

Y

G

Q

E

G

H

L

I

A

E

A

G

A

V

L

L

A

R

A

Q

E

V

N

N

-

R

-

L

-

T

-

G

T

C

Q

Y

L

L

R

H

L

L

F

Y

A

G

K
|
K

P

E

E

E

I

A

D

K

G

A

R

Q

R
|
R

R
x
K

L

M

G

G

V

H

A

V

L

N

A

I

R

L

D

N

K

D

D

N

I

I

D

E

S

V

A

A

V

L

N

E

K

K

A

A

active site: Y153 (vs. gap), G155 (= G147), E255 (= E252), E268 (= E264), N275 (≠ D271), S276 (≠ T272), K348 (≠ R349)
binding adenosine-5'-triphosphate: E76 (= E67), F77 (≠ I68), R107 (≠ E100), K147 (= K140), Y153 (vs. gap), D154 (≠ S146), G155 (= G147), Q158 (= Q150), W184 (≠ F182), V185 (≠ I183), F187 (= F185), E190 (= E188), E255 (= E252), F257 (= F254), N267 (≠ S263), E268 (= E264), R272 (= R268), H274 (= H270), N275 (≠ D271), K340 (= K341), R347 (= R348), K348 (≠ R349)
binding calcium ion: E255 (= E252), E268 (= E264)
binding phosphate ion: Q47 (= Q36), A49 (= A38)

3orqA Crystal structure of n5-carboxyaminoimidazole synthetase from staphylococcus aureus complexed with adp (see paper)
26% identity, 78% coverage: 7:299/378 of query aligns to 21:304/373 of 3orqA

query
sites
3orqA

E

Q

L

L

G

G

K

K

E

M

V

M

A

A

I

Q

E

S

L

A

Q

Q

R

K

L

M

G

G

V

Y

E

K

V

V

I

V

G

V

V

L

D

D

R

P

Y

S

P

E

N

D

A

C

P

P

A

C

M

R

Q

Y

I

V

A

A

H

H

R

E

S

F

H

I

V

Q

I

A

N

K

M

Y

L

D

D

D

A

E

K

K

A

A

L

L

R

N

A

Q

V

L

I

-

E

-

L

G

E

Q

K

K

P

C

H

D

L

V

V

I

I

T

P

Y

E

E

I

F

E

E

A

N

I

I

A

S

T

A

Q

Q

T

Q

L

L

V

-

E

K

M

L

E

L

A

C

E

E

G

K

L

Y

N

N

V

I

V

P

P

Q

T

G

A

Y

R

Q

A

A

T

I

K

Q

L

L

T

L

M

Q

D

D

R
|
R

E

-

G

L

I

T

R

E

R

K

L

E

A

T

A

L

E

K

T

S

L

A

G

G

L

T

P

K

T

V

S

V

P

P

Y

F

F

I

F

S

C

V

D

K

T

E

E

S

T

T

E

D

F

I

N

D

Q

K

A

A

I

I

S

E

K

T

I

L

G

G

V

Y

P

P

C

F

V

I

V

V

K
|
K

P

T

V

R

M

F

S

G

-
x
Y

S
x
D

G

G

K

K

G

G

Q
|
Q

S

V

V

L

I

I

R

N

N

N

V

E

A

K

Q

D

S

-

A

-

K

-

A

-

W

-

Q

-

Y

-

A

L

Q

Q

E

E

G

G

G

F

R

K

A

L

-

I

G

E

G

T

G

S

R

E

V

C

I

V

V

A

E
|
E

G

K

F
x
Y

I
x
L

P

N

F
x
I

D

K

Y

K

E
|
E

I

V

T

S

L

L

-

T

L

V

T

T

I

R

S

G

A

N

V

N

N

N

G

Q

I

I

H

T

F

F

C

F

A

P

P

L

I

Q

G

E

H

N

R

E

Q
x
H

E

R

D

N

G

Q

D

I

Y

L

R

F

E

K

S

T

W

I

Q

V

P

P

Q

A

A

R

M

I

S

D

A

K

D

T

V

A

L

E

A

A

K

K

S

E

Q

Q

A

V

I

-

A

-

S

N

K

K

V

I

E

Q

A

S

L

I

G

H

G

F

Y

I

G

G

L

T

F

F

G

T

V

V

E
|
E

L

F

F
|
F

V

I

K

D

G

S

S

N

D

N

-

Q

V

L

Y

Y

F

V

S

N

E
|
E

V

I

S

A

P

P

R

R

P

P

H

H

D
x
N

T
x
S

G

G

L

H

V

Y

T

S

L

I

I

E

S

A

Q

C

D

D

L

Y

S

S

E

Q

F

F

A

D

L

T

H

H

V

I

R

L

A

A

I

V

L

T

G

G

L

Q

P

S

I

L

P

P

N

N

active site: Y156 (vs. gap), E256 (= E252), E269 (= E264), N276 (≠ D271), S277 (≠ T272)
binding adenosine-5'-diphosphate: R110 (= R99), K150 (= K140), Y156 (vs. gap), D157 (≠ S146), Q161 (= Q150), E185 (= E180), Y187 (≠ F182), L188 (≠ I183), I190 (≠ F185), E193 (= E188), H216 (≠ Q210), F258 (= F254), E269 (= E264)
binding magnesium ion: E256 (= E252), E269 (= E264)
binding pyrophosphate 2-: Y156 (vs. gap)

Sites not aligning to the query:

active site: 350
binding pyrophosphate 2-: 342, 349

5jqwA The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with adp
30% identity, 56% coverage: 84:295/378 of query aligns to 83:290/365 of 5jqwA

query
sites
5jqwA

L

V

N

S

V

V

V

Y

P

P

T

S

A

A

R

K

A

A

T

I

K

A

L

I

T

S

M

Q

D

D

R
|
R

E

L

G

-

I

L

R

E

R

K

L

S

A

F

A

M

E

Q

T

D

L

H

G

G

L

I

P

A

T

T

S

A

P

K

Y

F

F

V

F

N

C

I

D

D

T

S

E

L

T

A

E

K

F

L

N

Q

Q

S

A

A

I

V

S

D

K

D

I

H

G

G

V

L

P

P

C

A

V

I

V

L

K
|
K

P

T

V

R

-

R

M

F

S
x
G

S
x
Y

S
x
D

G
|
G

K

K

G

G

Q

Q

S

F

V

V

I

I

R

R

N

S

V

Q

A

E

Q

D

S

I

A

T

K

K

A

A

W

W

Q

D

Y

V

A

L

Q

K

E

D

G

A

G

P

R

D

A

G

G

-

R

-

V

L

I

I

V

Y

E

E

G

A

F

F

I
x
V

P

D

F

F

D

D

Y

Y

E
|
E

I

V

T

S

L

Q

L

I

T

C

I

T

S

A

A

D

V

L

N

K

G

G

-

N

I

I

H

A

F

F

C

Y

A

P

P

L

I

A

G

R

H

N

R

T

Q
x
H

E

K

D

Q

G

G

D

I

Y

I

R

V

E

E

S

S

W

E

Q

A

P

P

Q

-

A

-

M

F

S

E

A

N

D

V

V

V

L

L

A

A

-

E

K

K

S

A

Q

Q

A

Q

I

I

A

A

S

K

K

I

V

L

V

V

E

K

A

E

L

F

G

A

G

Y

Y

V

G

G

L

T

F

L

G

A

V

I

E
|
E

L

F

F
|
F

V

V

K

K

G

G

S

D

D

E

V

L

Y

I

F

V

S

N

E
|
E

V

I

S

A

P

P

R

R

P

V

H

H

D
x
N

T
x
S

G

G

L

H

V

W

T

S

L

I

I

D

S

G

Q

A

D

V

L

T

S

S

E

Q

F

F

A

E

L

N

H

H

V

V

R

R

A

A

I

I

L

A

G

G

L

L

active site: Y144 (≠ S145), G146 (= G147), E247 (= E252), E259 (= E264), N266 (≠ D271), S267 (≠ T272)
binding adenosine-5'-diphosphate: R98 (= R99), K138 (= K140), G143 (≠ S144), Y144 (≠ S145), D145 (≠ S146), G146 (= G147), V178 (≠ I183), E183 (= E188), H206 (≠ Q210), F249 (= F254), E259 (= E264)

Sites not aligning to the query:

active site: 335

4mamA The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with an adp analog, amp-cp
30% identity, 56% coverage: 84:295/378 of query aligns to 83:290/373 of 4mamA

query
sites
4mamA

L

V

N

S

V

V

V

Y

P

P

T

S

A

A

R

K

A

A

T

I

K

A

L

I

T

S

M

Q

D

D

R
|
R

E

L

G

-

I

L

R

E

R

K

L

S

A

F

A

M

E

Q

T

D

L

H

G

G

L

I

P

A

T

T

S

A

P

K

Y

F

F

V

F

N

C

I

D

D

T

S

E

L

T

A

E

K

F

L

N

Q

Q

S

A

A

I

V

S

D

K

D

I

H

G

G

V

L

P

P

C

A

V
x
I

V

L

K
|
K

P

T

V

R

-

R

M

F

S

G

S
x
Y

S

D

G
|
G

K

K

G

G

Q
|
Q

S

F

V

V

I

I

R

R

N

S

V

Q

A

E

Q

D

S

I

A

T

K

K

A

A

W

W

Q

D

Y

V

A

L

Q

K

E

D

G

A

G

P

R

D

A

G

G

-

R

-

V

L

I

I

V

Y

E
|
E

G

A

F
|
F

I
x
V

P

D

F
|
F

D

D

Y

Y

E
|
E

I

V

T

S

L

Q

L

I

T

C

I

T

S

A

A

D

V

L

N

K

G

G

-

N

I

I

H

A

F

F

C

Y

A

P

P

L

I

A

G

R

H

N

R

T

Q
x
H

E

K

D

Q

G

G

D

I

Y

I

R

V

E

E

S

S

W

E

Q

A

P

P

Q

-

A

-

M

F

S

E

A

N

D

V

V

V

L

L

A

A

-

E

K

K

S

A

Q

Q

A

Q

I

I

A

A

S

K

K

I

V

L

V

V

E

K

A

E

L

F

G

A

G

Y

Y

V

G

G

L

T

F

L

G

A

V

I

E
|
E

L

F

F
|
F

V

V

K

K

G

G

S

D

D

E

V

L

Y

I

F

V

S

N

E
|
E

V

I

S

A

P

P

R

R

P

V

H

H

D
x
N

T
x
S

G

G

L

H

V

W

T

S

L

I

I

D

S

G

Q

A

D

V

L

T

S

S

E

Q

F

F

A

E

L

N

H

H

V

V

R

R

A

A

I

I

L

A

G

G

L

L

active site: Y144 (≠ S145), G146 (= G147), E247 (= E252), E259 (= E264), N266 (≠ D271), S267 (≠ T272)
binding phosphomethylphosphonic acid adenosyl ester: R98 (= R99), I136 (≠ V138), K138 (= K140), Y144 (≠ S145), G146 (= G147), Q149 (= Q150), E175 (= E180), F177 (= F182), V178 (≠ I183), F180 (= F185), E183 (= E188), H206 (≠ Q210), F249 (= F254), E259 (= E264)

Sites not aligning to the query:

active site: 335
binding phosphomethylphosphonic acid adenosyl ester: 371

4ma0A The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with partially hydrolysed atp
30% identity, 56% coverage: 84:295/378 of query aligns to 83:290/366 of 4ma0A

query
sites
4ma0A

L

V

N

S

V

V

V

Y

P

P

T

S

A

A

R

K

A

A

T

I

K

A

L

I

T

S

M

Q

D

D

R

R

E

L

G

-

I

L

R

E

R

K

L

S

A

F

A

M

E

Q

T

D

L

H

G

G

L

I

P

A

T

T

S

A

P

K

Y

F

F

V

F

N

C

I

D

D

T

S

E

L

T

A

E

K

F

L

N

Q

Q

S

A

A

I

V

S

D

K

D

I

H

G

G

V

L

P

P

C

A

V
x
I

V

L

K
|
K

P

T

V

R

-

R

M

F

S

G

S
x
Y

S

D

G
|
G

K

K

G

G

Q

Q

S

F

V

V

I

I

R

R

N

S

V

Q

A

E

Q

D

S

I

A

T

K

K

A

A

W

W

Q

D

Y

V

A

L

Q

K

E

D

G

A

G

P

R

D

A

G

G

-

R

-

V

L

I

I

V

Y

E
|
E

G
x
A

F
|
F

I
x
V

P

D

F

F

D

D

Y

Y

E
|
E

I

V

T

S

L

Q

L

I

T

C

I

T

S

A

A

D

V

L

N

K

G

G

-

N

I

I

H

A

F

F

C

Y

A

P

P

L

I

A

G

R

H

N

R

T

Q
x
H

E

K

D

Q

G

G

D

I

Y

I

R

V

E

E

S

S

W

E

Q

A

P

P

Q

-

A

-

M

F

S

E

A

N

D

V

V

V

L

L

A

A

-

E

K

K

S

A

Q

Q

A

Q

I

I

A

A

S

K

K

I

V

L

V

V

E

K

A

E

L

F

G

A

G

Y

Y

V

G

G

L

T

F

L

G

A

V

I

E
|
E

L

F

F
|
F

V

V

K

K

G

G

S

D

D

E

V

L

Y

I

F

V

S

N

E
|
E

V

I

S

A

P

P

R

R

P

V

H

H

D
x
N

T
x
S

G

G

L

H

V

W

T

S

L

I

I

D

S

G

Q

A

D

V

L

T

S

S

E

Q

F

F

A

E

L

N

H

H

V

V

R

R

A

A

I

I

L

A

G

G

L

L

active site: Y144 (≠ S145), G146 (= G147), E247 (= E252), E259 (= E264), N266 (≠ D271), S267 (≠ T272)
binding adenosine monophosphate: I136 (≠ V138), K138 (= K140), E175 (= E180), A176 (≠ G181), F177 (= F182), V178 (≠ I183), E183 (= E188), H206 (≠ Q210), F249 (= F254), E259 (= E264)

Sites not aligning to the query:

active site: 335

Query Sequence

>202710 FitnessBrowser__MR1:202710
MLLGCGELGKEVAIELQRLGVEVIGVDRYPNAPAMQIAHRSHVINMLDAKALRAVIELEK
PHLVIPEIEAIATQTLVEMEAEGLNVVPTARATKLTMDREGIRRLAAETLGLPTSPYFFC
DTETEFNQAISKIGVPCVVKPVMSSSGKGQSVIRNVAQSAKAWQYAQEGGRAGGGRVIVE
GFIPFDYEITLLTISAVNGIHFCAPIGHRQEDGDYRESWQPQAMSADVLAKSQAIASKVV
EALGGYGLFGVELFVKGSDVYFSEVSPRPHDTGLVTLISQDLSEFALHVRAILGLPIPNI
HQHGPSASAVVLVEGKSKNIRYQGLAAALAAENTQLRLFAKPEIDGRRRLGVALARDKDI
DSAVNKALDSAAKVQVIF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory