SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 203312 FitnessBrowser__MR1:203312 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0A6F3 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Escherichia coli (strain K12) (see 10 papers)
72% identity, 99% coverage: 2:491/494 of query aligns to 3:493/502 of P0A6F3

query
sites
P0A6F3

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T14 (= T13) binding ; binding
R18 (= R17) binding
S59 (= S58) mutation to W: Abolishes inhibition of GK by FBP via disruption of the dimer-tetramer assembly reaction. Inhibition by EIIA-Glc is unchanged compared to wild type. The activity of this mutant is significantly higher than wild-type, and the Michaelis constants are increased slightly compared to wild-type.
A66 (= A65) mutation to T: Although it completely abolishes FBP regulation and disrupts dimer-tetramer equilibrium, the crystal structure is essentially identical to the symmetric tetramer found in the FBP-bound form of the enzyme.
R84 (= R83) binding ; binding
E85 (= E84) binding ; binding
Y136 (= Y135) binding ; binding
G231 (≠ A230) mutation to D: Displays an increased enzymatic activity and a decreased allosteric regulation by FBP compared to wild-type. It displays a dimer form and is resistant to tetramer formation in the presence of FBP, whereas wild-type dimers are converted into inactive tetramers in the presence of FBP.
K233 (≠ E232) modified: N6-malonyllysine
G235 (= G233) binding
R237 (≠ E235) binding ; mutation to A: Drastically reduces inhibition of GK by FBP and lowers, but did not eliminate, the ability of FBP to promote tetramer association.
D246 (= D244) binding ; binding
Q247 (= Q245) binding
T268 (= T266) binding
G305 (= G303) mutation to S: In glpK22; abolishes glucose control of glycerol utilization.
G311 (= G309) binding
G412 (= G410) binding
N416 (= N414) binding
I475 (= I473) mutation to D: It decreases Vmax to about 10% of the wild-type value and the affinity for substrate is increased about two- to fourfold. This mutation decreases the catalytic activity in a manner that is analogous to that obtained upon EIIA-Glc binding. It increases the affinity for FBP about fivefold.
E479 (≠ Y477) binding
R480 (= R478) mutation to D: It decreases Vmax to about 10% of the wild-type value and the affinity for substrate is increased about two- to fourfold. This mutation decreases the catalytic activity in a manner that is analogous to that obtained upon EIIA-Glc binding. Regulation by FBP is not affected by this substitution. No inhibition by EIIA-Glc is observed, which is consistent with a decrease in affinity for EIIA-Glc of about 250-fold.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1glfO Crystal structures of escherichia coli glycerol kinase and the mutant a65t in an inactive tetramer: conformational changes and implications for allosteric regulation (see paper)
72% identity, 99% coverage: 2:491/494 of query aligns to 1:491/498 of 1glfO

query
sites
1glfO

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binding adenosine-5'-diphosphate: R16 (= R17), G265 (= G265), T266 (= T266), G309 (= G309), G410 (= G410), A411 (= A411)
binding glycerol: R82 (= R83), E83 (= E84), Y134 (= Y135), D244 (= D244)
binding phosphate ion: G232 (vs. gap), G233 (= G233), R235 (≠ E235)

1bo5O Crystal structure of the complex between escherichia coli glycerol kinase and the allosteric regulator fructose 1,6-bisphosphate. (see paper)
72% identity, 99% coverage: 2:491/494 of query aligns to 1:491/498 of 1bo5O

query
sites
1bo5O

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T

T

N

N

A

A

A

S

R

R

T

T

L

M

L

L

F

F

N

N

I

I

H

H

S

T

L

L

S

D

W

W

D

D

S

D

K

K

L

M

L

L

E

E

A

V

L

L

D

D

I

I

P

P

A

R

A

E

M

M

L

L

P

P

E

E

V

V

K

R

P

R

S

S

C

S

S

E

V

V

Y

Y

G

G

T

Q

T

T

R

N

I

I

A

G

G

G

E

K

-
x
G

G
|
G

S

T

E
x
R

I

I

P

P

L

I

A

S

G

G

I

I

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

L

L

C

C

V

V

E

K

Q

E

G

G

M

M

A

A

K

K

N

N

T

T

Y

Y

G

G

T

T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

S

E

K

K

A

A

V

V

R

K

S

S

S

E

H

N

G

G

L

L

L

L

T

T

T

T

V

I

A

A

V

C

G

G

A

P

Q

T

G

G

E

E

V

V

N

N

Y

Y

A

A

L

L

E

E

G

G

S

A

V

V

F

F

M

M

G

A

G

G

A

A

T

S

I

I

Q

Q

W

W

L

L

R

R

D

D

E

E

L

M

G

K

L

L

I

I

R

N

D

D

A

A

S

Y

D

D

T

S

E

E

Y

Y

F

F

A

A

S

T

K

K

V

V

A

Q

D

N

T

T

N

N

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

P

N

Y

A

A

R

R

G

G

A

A

L

I

F

F

G

G

L

L

T

T

R

R

G

G

A

V

N

N

R

A

N

N

H

H

I

I

I

I

R

R

A

A

A

T

L

L

E

E

S

S

I

I

A

A

Y

Y

Q

Q

S

T

K

R

D

D

L

V

L

L

D

E

A

A

M

M

I

Q

K

A

D

D

S

S

G

G

V

I

S

R

L

L

K

H

R

A

L

L

K

R

V

V

D

D

G

G

G

G

A

A

V

V

A

A

N

N

D

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

A

S

D

D

I

I

T

L

D

G

V

T

E

R

V

V

L

E

R

R

P

P

S

E

V

V

C

R

E

E

T

V

T

T

A

A

L

L

G

G

A

A

A

A

F

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

N

Q

S

N

V

L

T

D

E

E

L

L

E

Q

H

E

K

K

A

A

C

V

I

I

D

E

T

R

H

E

F

F

K

R

P

P

S

G

I

I

N

E

A

T

D

T

Y

E

R

R

Q

N

Q

Y

L

R

Y

Y

A

A

G

G

W

W

Q

K

D

K

A

A

V

V

A

K

R

R

binding 1,6-di-O-phosphono-beta-D-fructofuranose: G232 (vs. gap), G233 (= G233), R235 (≠ E235)
binding glycerol: R82 (= R83), E83 (= E84), W102 (= W103), Y134 (= Y135), D244 (= D244), Q245 (= Q245), F269 (= F269)

1bu6Y Crystal structures of escherichia coli glycerol kinase and the mutant a65t in an inactive tetramer: conformational changes and implications for allosteric regulation (see paper)
72% identity, 99% coverage: 2:491/494 of query aligns to 1:491/499 of 1bu6Y

query
sites
1bu6Y

Q

E

K

K

K

K

Y

Y

V

I

V

V

A

A

L

L

D

D

Q

Q

G

G

T

T

T

T

S

S

S

S

R

R

A

A

I

V

V

V

F

M

D

D

H

H

D

D

A

A

N

N

I

I

V

I

S

S

V

V

S

S

Q

Q

R

R

E

E

F

F

T

E

Q

Q

L

I

Y

Y

P

P

N

K

P

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

M

M

E

E

I

I

W

W

A

A

S

T

Q

Q

S

S

S

S

V

T

L

L

I

V

E

E

S

V

L

L

A

T

R

K

A

A

G

D

I

I

H

S

S

S

D

D

A

Q

V

I

A

A

A

A

I

I

G

G

I

I

T

T

N

N

Q
|
Q

R
|
R

E
|
E

T

T

T

T

I

I

I

V

W

W

E

E

K

K

A

E

T

T

G

G

K

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W
|
W

Q

Q

C

C

R

R

R

R

S

T

A

A

E

E

I

I

C

C

E

E

Q

H

L

L

K

K

A

R

Q

D

G

G

L

L

E

E

D

D

Y

Y

V

I

R

R

Q

S

N

N

T

T

G

G

L

L

L

V

L

I

D

D

P

P

Y
|
Y

F

F

S

S

G

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

P

E

D

G

A

S

R

R

A

E

K

R

A

A

K

R

R

R

G

G

E

E

L

L

L

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

L

I

W

W

K

K

L

M

T

T

E

Q

G

G

K

R

V

V

H

H

V

V

T

T

D

D

P

Y

T

T

N

N

A

A

A

S

R

R

T

T

L

M

L

L

F

F

N

N

I

I

H

H

S

T

L

L

S

D

W

W

D

D

S

D

K

K

L

M

L

L

E

E

A

V

L

L

D

D

I

I

P

P

A

R

A

E

M

M

L

L

P

P

E

E

V

V

K

R

P

R

S

S

C

S

S

E

V

V

Y

Y

G

G

T

Q

T

T

R

N

I

I

A

G

G

G

E

K

-

G

G

G

S

T

E

R

I

I

P

P

L

I

A

S

G

G

I

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

L

L

C

C

V

V

E

K

Q

E

G

G

M

M

A

A

K

K

N

N

T

T

Y

Y

G

G

T

T

G

G

C

C

F

F

L

M

L

L

M

M

N

N

T

T

G

G

S

E

K

K

A

A

V

V

R

K

S

S

S

E

H

N

G

G

L

L

L

L

T

T

T

T

V

I

A

A

V

C

G

G

A

P

Q

T

G

G

E

E

V

V

N

N

Y

Y

A

A

L

L

E

E

G

G

S

A

V

V

F

F

M

M

G

A

G

G

A

A

T

S

I

I

Q

Q

W

W

L

L

R

R

D

D

E

E

L

M

G

K

L

L

I

I

R

N

D

D

A

A

S

Y

D

D

T

S

E

E

Y

Y

F

F

A

A

S

T

K

K

V

V

A

Q

D

N

T

T

N

N

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

P

N

Y

A

A

R

R

G

G

A

A

L

I

F

F

G

G

L

L

T

T

R

R

G

G

A

V

N

N

R

A

N

N

H

H

I

I

I

I

R

R

A

A

A

T

L

L

E

E

S

S

I

I

A

A

Y

Y

Q

Q

S

T

K

R

D

D

L

V

L

L

D

E

A

A

M

M

I

Q

K

A

D

D

S

S

G

G

V

I

S

R

L

L

K

H

R

A

L

L

K

R

V

V

D

D

G

G

G

G

A

A

V

V

A

A

N

N

D

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

A

S

D

D

I

I

T

L

D

G

V

T

E

R

V

V

L

E

R

R

P

P

S

E

V

V

C

R

E

E

T

V

T

T

A

A

L

L

G

G

A

A

A

A

F

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

N

Q

S

N

V

L

T

D

E

E

L

L

E

Q

H

E

K

K

A

A

C

V

I

I

D

E

T

R

H

E

F

F

K

R

P

P

S

G

I

I

N

E

A

T

D

T

Y

E

R

R

Q

N

Q

Y

L

R

Y

Y

A

A

G

G

W

W

Q

K

D

K

A

A

V

V

A

K

R

R

binding glycerol: Q81 (= Q82), R82 (= R83), E83 (= E84), W102 (= W103), Y134 (= Y135), D244 (= D244)

1gllO Escherichia coli glycerol kinase mutant with bound atp analog showing substantial domain motion (see paper)
72% identity, 99% coverage: 2:491/494 of query aligns to 1:487/494 of 1gllO

query
sites
1gllO

Q

E

K

K

K

K

Y

Y

V

I

V

V

A

A

L

L

D

D

Q

Q

G

G

T
|
T

T
|
T

S

S

S

S

R

R

A

A

I

V

V

V

F

M

D

D

H

H

D

D

A

A

N

N

I

I

V

I

S

S

V

V

S

S

Q

Q

R

R

E

E

F

F

T

E

Q

Q

L

I

Y

Y

P

P

N

K

P

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

M

M

E

E

I

I

W

W

A

A

S

T

Q

Q

S

S

S

W

V

T

L

L

I

V

E

E

S

V

L

L

A

A

R

K

A

A

G

D

I

I

H

S

S

S

D

D

A

Q

V

I

A

A

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

I

V

W

W

E

E

K

K

A

E

T

T

G

G

K

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

C

C

R

R

R

R

S

T

A

A

E

E

I

I

C

C

E

E

Q

H

L

L

K

K

A

R

Q

D

G

G

L

L

E

E

D

D

Y

Y

V

I

R

R

Q

S

N

N

T

T

G

G

L

L

L

V

L

I

D

D

P

P

Y
|
Y

F

F

S

S

G

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

P

E

D

G

A

S

R

R

A

E

K

R

A

A

K

R

R

R

G

G

E

E

L

L

L

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

L

I

W

W

K

K

L

M

T

T

E

Q

G

G

K

R

V

V

H

H

V

V

T

T

D

D

P

Y

T

T

N

N

A

A

A

S

R

R

T

T

L

M

L

L

F

F

N

N

I

I

H

H

S

T

L

L

S

D

W

W

D

D

S

D

K

K

L

M

L

L

E

E

A

V

L

L

D

D

I

I

P

P

A

R

A

E

M

M

L

L

P

P

E

E

V

V

K

R

P

R

S

S

C

S

S

E

V

V

Y

Y

G

G

T

Q

T

T

R

N

I

I

A

-

G

-

E

-

G

G

S

T

E

R

I

I

P

P

L

I

A

S

G

G

I

I

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

L

L

C

C

V

V

E

K

Q

E

G

G

M

M

A

A

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

S

E

K

K

A

A

V

V

R

K

S

S

S

E

H

N

G

G

L

L

L

L

T

T

T

T

V

I

A

A

V

C

G

G

A

P

Q

T

G

G

E

E

V

V

N

N

Y

Y

A

A

L

L

E

E

G

G

S

A

V

V

F

F

M

M

G

A

G
|
G

A

A

T

S

I
|
I

Q
|
Q

W

W

L

L

R

R

D

D

E

E

L

M

G

K

L

L

I

I

R

N

D

D

A
|
A

S

Y

D

D

T

S

E

E

Y

Y

F

F

A

A

S

T

K

K

V

V

A

Q

D

N

T

T

N

N

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

P

N

Y

A

A

R

R

G

G

A

A

L

I

F

F

G

G

L

L

T

T

R

R

G

G

A

V

N

N

R

A

N

N

H

H

I

I

I

I

R

R

A

A

A

T

L

L

E

E

S

S

I

I

A

A

Y

Y

Q

Q

S

T

K

R

D

D

L

V

L

L

D

E

A

A

M

M

I

Q

K

A

D

D

S

S

G

G

V

I

S

R

L

L

K

H

R

A

L

L

K

R

V

V

D

D

G

G

G
|
G

A

A

V

V

A

A

N
|
N

D

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

A

S

D

D

I

I

T

L

D

G

V

T

E

R

V

V

L

E

R

R

P

P

S

E

V

V

C

R

E

E

T

V

T

T

A

A

L

L

G

G

A

A

A

A

F

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

N

Q

S

N

V

L

T

D

E

E

L

L

E

Q

H

E

K

K

A

A

C

V

I

I

D

E

T

R

H

E

F

F

K

R

P

P

S

G

I

I

N

E

A

T

D

T

Y

E

R

R

Q

N

Q

Y

L

R

Y

Y

A

A

G

G

W

W

Q

K

D

K

A

A

V

V

A

K

R

R

binding phosphomethylphosphonic acid adenylate ester: T12 (= T13), T13 (= T14), G261 (= G265), T262 (= T266), G305 (= G309), I308 (= I312), Q309 (= Q313), A321 (= A325), G406 (= G410), N410 (= N414)
binding glycerol: R82 (= R83), E83 (= E84), Y134 (= Y135), D240 (= D244), Q241 (= Q245), F265 (= F269)

1gljO Escherichia coli glycerol kinase mutant with bound atp analog showing substantial domain motion (see paper)
72% identity, 99% coverage: 2:491/494 of query aligns to 1:487/494 of 1gljO

query
sites
1gljO

Q

E

K

K

K

K

Y

Y

V

I

V

V

A

A

L

L

D

D

Q

Q

G

G

T
|
T

T
|
T

S

S

S

S

R

R

A

A

I

V

V

V

F

M

D

D

H

H

D

D

A

A

N

N

I

I

V

I

S

S

V

V

S

S

Q

Q

R

R

E

E

F

F

T

E

Q

Q

L

I

Y

Y

P

P

N

K

P

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

M

M

E

E

I

I

W

W

A

A

S

T

Q

Q

S

S

S

W

V

T

L

L

I

V

E

E

S

V

L

L

A

A

R

K

A

A

G

D

I

I

H

S

S

S

D

D

A

Q

V

I

A

A

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

I

V

W

W

E

E

K

K

A

E

T

T

G

G

K

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W
|
W

Q

Q

C

C

R

R

R

R

S

T

A

A

E

E

I

I

C

C

E

E

Q

H

L

L

K

K

A

R

Q

D

G

G

L

L

E

E

D

D

Y

Y

V

I

R

R

Q

S

N

N

T

T

G

G

L

L

L

V

L

I

D

D

P

P

Y
|
Y

F

F

S

S

G

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

P

E

D

G

A

S

R

R

A

E

K

R

A

A

K

R

R

R

G

G

E

E

L

L

L

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

L

I

W

W

K

K

L

M

T

T

E

Q

G

G

K

R

V

V

H

H

V

V

T

T

D

D

P

Y

T

T

N

N

A

A

A

S

R

R

T

T

L

M

L

L

F

F

N

N

I

I

H

H

S

T

L

L

S

D

W

W

D

D

S

D

K

K

L

M

L

L

E

E

A

V

L

L

D

D

I

I

P

P

A

R

A

E

M

M

L

L

P

P

E

E

V

V

K

R

P

R

S

S

C

S

S

E

V

V

Y

Y

G

G

T

Q

T

T

R

N

I

I

A

-

G

-

E

-

G

G

S

T

E

R

I

I

P

P

L

I

A

S

G

G

I

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

L

L

C

C

V

V

E

K

Q

E

G

G

M

M

A

A

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

S

E

K

K

A

A

V

V

R

K

S

S

S

E

H

N

G

G

L

L

L

L

T

T

T

T

V

I

A

A

V

C

G

G

A

P

Q

T

G

G

E

E

V

V

N

N

Y

Y

A

A

L

L

E

E

G

G

S

A

V

V

F

F

M

M

G

A

G
|
G

A

A

T

S

I

I

Q
|
Q

W

W

L

L

R

R

D

D

E

E

L

M

G

K

L

L

I

I

R

N

D

D

A
|
A

S

Y

D

D

T

S

E

E

Y

Y

F

F

A

A

S

T

K

K

V

V

A

Q

D

N

T

T

N

N

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

P

N

Y

A

A

R

R

G

G

A

A

L

I

F

F

G

G

L

L

T

T

R

R

G

G

A

V

N

N

R

A

N

N

H

H

I

I

I

I

R

R

A

A

A

T

L

L

E

E

S

S

I

I

A

A

Y

Y

Q

Q

S

T

K

R

D

D

L

V

L

L

D

E

A

A

M

M

I

Q

K

A

D

D

S

S

G

G

V

I

S

R

L

L

K

H

R

A

L

L

K

R

V

V

D

D

G

G

G
|
G

A
|
A

V

V

A

A

N

N

D

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

A

S

D

D

I

I

T

L

D

G

V

T

E

R

V

V

L

E

R

R

P

P

S

E

V

V

C

R

E

E

T

V

T

T

A

A

L

L

G

G

A

A

A

A

F

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

N

Q

S

N

V

L

T

D

E

E

L

L

E

Q

H

E

K

K

A

A

C

V

I

I

D

E

T

R

H

E

F

F

K

R

P

P

S

G

I

I

N

E

A

T

D

T

Y

E

R

R

Q

N

Q

Y

L

R

Y

Y

A

A

G

G

W

W

Q

K

D

K

A

A

V

V

A

K

R

R

binding gamma-arsono-beta, gamma-methyleneadenosine-5'-diphosphate: T12 (= T13), T13 (= T14), G261 (= G265), T262 (= T266), G305 (= G309), Q309 (= Q313), A321 (= A325), G406 (= G410), A407 (= A411)
binding glycerol: R82 (= R83), E83 (= E84), W102 (= W103), Y134 (= Y135), D240 (= D244), F265 (= F269)

1bwfO Escherichia coli glycerol kinase mutant with bound atp analog showing substantial domain motion (see paper)
72% identity, 99% coverage: 2:491/494 of query aligns to 1:487/494 of 1bwfO

query
sites
1bwfO

Q

E

K

K

K

K

Y

Y

V

I

V

V

A

A

L

L

D

D

Q

Q

G

G

T
|
T

T
|
T

S

S

S

S

R

R

A

A

I

V

V

V

F

M

D

D

H

H

D

D

A

A

N

N

I

I

V

I

S

S

V

V

S

S

Q

Q

R

R

E

E

F

F

T

E

Q

Q

L

I

Y

Y

P

P

N

K

P

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

M

M

E

E

I

I

W

W

A

A

S

T

Q

Q

S

S

S

W

V

T

L

L

I

V

E

E

S

V

L

L

A

A

R

K

A

A

G

D

I

I

H

S

S

S

D

D

A

Q

V

I

A

A

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

I

V

W

W

E

E

K

K

A

E

T

T

G

G

K

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W
|
W

Q

Q

C

C

R

R

R

R

S

T

A

A

E

E

I

I

C

C

E

E

Q

H

L

L

K

K

A

R

Q

D

G

G

L

L

E

E

D

D

Y

Y

V

I

R

R

Q

S

N

N

T

T

G

G

L

L

L

V

L

I

D

D

P

P

Y
|
Y

F

F

S

S

G

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

P

E

D

G

A

S

R

R

A

E

K

R

A

A

K

R

R

R

G

G

E

E

L

L

L

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

L

I

W

W

K

K

L

M

T

T

E

Q

G

G

K

R

V

V

H

H

V

V

T

T

D

D

P

Y

T

T

N

N

A

A

A

S

R

R

T

T

L

M

L

L

F

F

N

N

I

I

H

H

S

T

L

L

S

D

W

W

D

D

S

D

K

K

L

M

L

L

E

E

A

V

L

L

D

D

I

I

P

P

A

R

A

E

M

M

L

L

P

P

E

E

V

V

K

R

P

R

S

S

C

S

S

E

V

V

Y

Y

G

G

T

Q

T

T

R

N

I

I

A

-

G

-

E

-

G

G

S

T

E

R

I

I

P

P

L

I

A

S

G

G

I

I

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

L

L

C

C

V

V

E

K

Q

E

G

G

M

M

A

A

K

K

N

N

T

T

Y

Y

G

G

T
|
T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

S

E

K

K

A

A

V

V

R

K

S

S

S

E

H

N

G

G

L

L

L

L

T

T

T

T

V

I

A

A

V

C

G

G

A

P

Q

T

G

G

E

E

V

V

N

N

Y

Y

A

A

L

L

E

E

G

G

S

A

V

V

F

F

M

M

G

A

G
|
G

A

A

T

S

I
|
I

Q
|
Q

W

W

L

L

R

R

D

D

E

E

L

M

G

K

L

L

I

I

R

N

D

D

A
|
A

S

Y

D

D

T

S

E

E

Y

Y

F

F

A

A

S

T

K

K

V

V

A

Q

D

N

T

T

N

N

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

P

N

Y

A

A

R

R

G

G

A

A

L

I

F

F

G

G

L

L

T

T

R

R

G

G

A

V

N

N

R

A

N

N

H

H

I

I

I

I

R

R

A

A

A

T

L

L

E

E

S

S

I

I

A

A

Y

Y

Q

Q

S

T

K

R

D

D

L

V

L

L

D

E

A

A

M

M

I

Q

K

A

D

D

S

S

G

G

V

I

S

R

L

L

K

H

R

A

L

L

K

R

V

V

D

D

G

G

G
|
G

A

A

V

V

A

A

N
|
N

D

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

A

S

D

D

I

I

T

L

D

G

V

T

E

R

V

V

L

E

R

R

P

P

S

E

V

V

C

R

E

E

T

V

T

T

A

A

L

L

G

G

A

A

A

A

F

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

N

Q

S

N

V

L

T

D

E

E

L

L

E

Q

H

E

K

K

A

A

C

V

I

I

D

E

T

R

H

E

F

F

K

R

P

P

S

G

I

I

N

E

A

T

D

T

Y

E

R

R

Q

N

Q

Y

L

R

Y

Y

A

A

G

G

W

W

Q

K

D

K

A

A

V

V

A

K

R

R

binding phosphodifluoromethylphosphonic acid-adenylate ester: T12 (= T13), T13 (= T14), T262 (= T266), G305 (= G309), I308 (= I312), Q309 (= Q313), A321 (= A325), G406 (= G410), N410 (= N414)
binding glycerol: R82 (= R83), E83 (= E84), W102 (= W103), Y134 (= Y135), D240 (= D244), Q241 (= Q245), F265 (= F269)

1gldG Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
72% identity, 99% coverage: 4:491/494 of query aligns to 1:482/489 of 1gldG

query
sites
1gldG

K

K

Y

Y

V

I

V

V

A

A

L

L

D
|
D

Q

Q

G

G

T
|
T

T

T

S

S

S

S

R
|
R

A

A

I

V

V

V

F

M

D

D

H

H

D

D

A

A

N

N

I

I

V

I

S

S

V

V

S

S

Q

Q

R

R

E

E

F

F

T

E

Q

Q

L

I

Y

Y

P

P

N

K

P

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

M

M

E

E

I

I

W

W

A

A

S

T

Q

Q

S

S

S

S

V

T

L

L

I

V

E

E

S

V

L

L

A

A

R

K

A

A

G

D

I

I

H

S

S

S

D

D

A

Q

V

I

A

A

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

I

V

W

W

E

E

K

K

A

E

T

T

G

G

K

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

C

C

R

R

R

R

S

T

A

A

E

E

I

I

C

C

E

E

Q

H

L

L

K

K

A

R

Q

D

G

G

L

L

E

E

D

D

Y

Y

V

I

R

R

Q

S

N

N

T

T

G

G

L

L

L

V

L

I

D

D

P

P

Y
|
Y

F

F

S

S

G

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

P

E

D

G

A

S

R

R

A

E

K

R

A

A

K

R

R

R

G

G

E

E

L

L

L

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

L

I

W

W

K

K

L

M

T

T

E

Q

G

G

K

R

V

V

H

H

V

V

T

T

D

D

P

Y

T

T

N

N

A

A

A

S

R

R

T

T

L

M

L

L

F

F

N

N

I

I

H

H

S

T

L

L

S

D

W

W

D

D

S

D

K

K

L

M

L

L

E

E

A

V

L

L

D

D

I

I

P

P

A

R

A

E

M

M

L

L

P

P

E

E

V

V

K

R

P

R

S

S

C

S

S

E

V

V

Y

Y

G

G

T

Q

T

T

R

N

I

I

A

-

G

-

E

-

G

-

S

-

E

-

I

I

P

P

L

I

A

S

G

G

I

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

L

L

C

C

V

V

E

K

Q

E

G

G

M

M

A

A

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

S

E

K

K

A

A

V

V

R

K

S

S

S

E

H

N

G

G

L

L

L

L

T

T

T

T

V

I

A

A

V

C

G

G

A

P

Q

T

G

G

E

E

V

V

N

N

Y

Y

A

A

L

L

E

E

G

G

S

A

V

V

F

F

M

M

G

A

G
|
G

A

A

T

S

I

I

Q

Q

W

W

L

L

R

R

D

D

E

E

L

M

G

K

L

L

I

I

R

N

D

D

A
|
A

S

Y

D

D

T

S

E

E

Y

Y

F

F

A

A

S

T

K

K

V

V

A

Q

D

N

T

T

N

N

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

P

N

Y

A

A

R

R

G

G

A

A

L

I

F

F

G

G

L

L

T

T

R

R

G

G

A

V

N

N

R

A

N

N

H

H

I

I

I

I

R

R

A

A

A

T

L

L

E

E

S

S

I

I

A

A

Y

Y

Q

Q

S

T

K

R

D

D

L

V

L

L

D

E

A

A

M

M

I

Q

K

A

D

D

S

S

G

G

V

I

S

R

L

L

K

H

R

A

L

L

K

R

V

V

D

D

G

G

G
|
G

A
|
A

V

V

A

A

N
|
N

D

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

A

S

D

D

I

I

T

L

D

G

V

T

E

R

V

V

L

E

R

R

P

P

S

E

V

V

C

R

E

E

T

V

T

T

A

A

L

L

G

G

A

A

A

A

F

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

N

Q

S

N

V

L

T

D

E

E

L

L

E

Q

H

E

K

K

A

A

C

V

I

I

D

E

T

R

H

E

F

F

K

R

P

P

S

G

I

I

N

E

A

T

D

T

Y

E

R

R

Q

N

Q

Y

L

R

Y

Y

A

A

G

G

W

W

Q

K

D

K

A

A

V

V

A

K

R

R

binding adenosine-5'-diphosphate: R14 (= R17), G256 (= G265), T257 (= T266), G300 (= G309), A316 (= A325), G401 (= G410), A402 (= A411), N405 (= N414)
binding glyceraldehyde-3-phosphate: T10 (= T13), R80 (= R83), E81 (= E84), Y132 (= Y135), D235 (= D244), F260 (= F269)
binding manganese (ii) ion: D7 (= D10), R14 (= R17)

1glcG Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
72% identity, 99% coverage: 4:491/494 of query aligns to 1:482/489 of 1glcG

query
sites
1glcG

K

K

Y

Y

V

I

V

V

A

A

L

L

D

D

Q

Q

G

G

T
|
T

T

T

S

S

S

S

R

R

A

A

I

V

V

V

F

M

D

D

H

H

D

D

A

A

N

N

I

I

V

I

S

S

V

V

S

S

Q

Q

R

R

E

E

F

F

T

E

Q

Q

L

I

Y

Y

P

P

N

K

P

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

M

M

E

E

I

I

W

W

A

A

S

T

Q

Q

S

S

S

S

V

T

L

L

I

V

E

E

S

V

L

L

A

A

R

K

A

A

G

D

I

I

H

S

S

S

D

D

A

Q

V

I

A

A

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

I

V

W

W

E

E

K

K

A

E

T

T

G

G

K

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W
|
W

Q

Q

C

C

R

R

R

R

S

T

A

A

E

E

I

I

C

C

E

E

Q

H

L

L

K

K

A

R

Q

D

G

G

L

L

E

E

D

D

Y

Y

V

I

R

R

Q

S

N

N

T

T

G

G

L

L

L

V

L

I

D

D

P

P

Y
|
Y

F

F

S

S

G

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

P

E

D

G

A

S

R

R

A

E

K

R

A

A

K

R

R

R

G

G

E

E

L

L

L

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

L

I

W

W

K

K

L

M

T

T

E

Q

G

G

K

R

V

V

H

H

V

V

T

T

D

D

P

Y

T

T

N

N

A

A

A

S

R

R

T

T

L

M

L

L

F

F

N

N

I

I

H

H

S

T

L

L

S

D

W

W

D

D

S

D

K

K

L

M

L

L

E

E

A

V

L

L

D

D

I

I

P

P

A

R

A

E

M

M

L

L

P

P

E

E

V

V

K

R

P

R

S

S

C

S

S

E

V

V

Y

Y

G

G

T

Q

T

T

R

N

I

I

A

-

G

-

E

-

G

-

S

-

E

-

I

I

P

P

L

I

A

S

G

G

I

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

L

L

C

C

V

V

E

K

Q

E

G

G

M

M

A

A

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

S

E

K

K

A

A

V

V

R

K

S

S

S

E

H

N

G

G

L

L

L

L

T

T

T

T

V

I

A

A

V

C

G

G

A

P

Q

T

G

G

E

E

V

V

N

N

Y

Y

A

A

L

L

E

E

G

G

S

A

V

V

F

F

M

M

G

A

G
|
G

A

A

T

S

I

I

Q

Q

W

W

L

L

R

R

D

D

E

E

L

M

G

K

L

L

I

I

R

N

D

D

A
|
A

S

Y

D

D

T

S

E

E

Y

Y

F

F

A

A

S

T

K

K

V

V

A

Q

D

N

T

T

N

N

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

P

N

Y

A

A

R

R

G

G

A

A

L

I

F

F

G

G

L

L

T

T

R

R

G

G

A

V

N

N

R

A

N

N

H

H

I

I

I

I

R

R

A

A

A

T

L

L

E

E

S

S

I

I

A

A

Y

Y

Q

Q

S

T

K

R

D

D

L

V

L

L

D

E

A

A

M

M

I

Q

K

A

D

D

S

S

G

G

V

I

S

R

L

L

K

H

R

A

L

L

K

R

V

V

D

D

G

G

G
|
G

A
|
A

V

V

A

A

N
|
N

D

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

A

S

D

D

I

I

T

L

D

G

V

T

E

R

V

V

L

E

R

R

P

P

S

E

V

V

C

R

E

E

T

V

T

T

A

A

L

L

G

G

A

A

A

A

F

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

N

Q

S

N

V

L

T

D

E

E

L

L

E

Q

H

E

K

K

A

A

C

V

I

I

D

E

T

R

H

E

F

F

K

R

P

P

S

G

I

I

N

E

A

T

D

T

Y

E

R

R

Q

N

Q

Y

L

R

Y

Y

A

A

G

G

W

W

Q

K

D

K

A

A

V

V

A

K

R

R

binding adenosine-5'-diphosphate: G256 (= G265), T257 (= T266), G300 (= G309), A316 (= A325), G401 (= G410), A402 (= A411), N405 (= N414)
binding glyceraldehyde-3-phosphate: T10 (= T13), R80 (= R83), E81 (= E84), W100 (= W103), Y132 (= Y135), D235 (= D244), F260 (= F269)

1glbG Structure of the regulatory complex of escherichia coli iiiglc with glycerol kinase (see paper)
72% identity, 99% coverage: 4:491/494 of query aligns to 1:482/489 of 1glbG

query
sites
1glbG

K

K

Y

Y

V

I

V

V

A

A

L

L

D

D

Q

Q

G

G

T

T

T

T

S

S

S

S

R
|
R

A

A

I

V

V

V

F

M

D

D

H

H

D

D

A

A

N

N

I

I

V

I

S

S

V

V

S

S

Q

Q

R

R

E

E

F

F

T

E

Q

Q

L

I

Y

Y

P

P

N

K

P

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

M

M

E

E

I

I

W

W

A

A

S

T

Q

Q

S

S

S

S

V

T

L

L

I

V

E

E

S

V

L

L

A

A

R

K

A

A

G

D

I

I

H

S

S

S

D

D

A

Q

V

I

A

A

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

I

V

W

W

E

E

K

K

A

E

T

T

G

G

K

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W
|
W

Q

Q

C

C

R

R

R

R

S

T

A

A

E

E

I

I

C

C

E

E

Q

H

L

L

K

K

A

R

Q

D

G

G

L

L

E

E

D

D

Y

Y

V

I

R

R

Q

S

N

N

T

T

G

G

L

L

L

V

L

I

D

D

P

P

Y
|
Y

F

F

S

S

G

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

P

E

D

G

A

S

R

R

A

E

K

R

A

A

K

R

R

R

G

G

E

E

L

L

L

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

L

I

W

W

K

K

L

M

T

T

E

Q

G

G

K

R

V

V

H

H

V

V

T

T

D

D

P

Y

T

T

N

N

A

A

A

S

R

R

T

T

L

M

L

L

F

F

N

N

I

I

H

H

S

T

L

L

S

D

W

W

D

D

S

D

K

K

L

M

L

L

E

E

A

V

L

L

D

D

I

I

P

P

A

R

A

E

M

M

L

L

P

P

E

E

V

V

K

R

P

R

S

S

C

S

S

E

V

V

Y

Y

G

G

T

Q

T

T

R

N

I

I

A

-

G

-

E

-

G

-

S

-

E

-

I

I

P

P

L

I

A

S

G

G

I

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

L

L

C

C

V

V

E

K

Q

E

G

G

M

M

A

A

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

S

E

K

K

A

A

V

V

R

K

S

S

S

E

H

N

G

G

L

L

L

L

T

T

T

T

V

I

A

A

V

C

G

G

A

P

Q

T

G

G

E

E

V

V

N

N

Y

Y

A

A

L

L

E

E

G

G

S

A

V

V

F

F

M

M

G

A

G
|
G

A

A

T

S

I
|
I

Q

Q

W

W

L

L

R

R

D

D

E

E

L

M

G

K

L

L

I

I

R

N

D

D

A
|
A

S

Y

D

D

T

S

E

E

Y

Y

F

F

A

A

S

T

K

K

V

V

A

Q

D

N

T

T

N

N

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

P

N

Y

A

A

R

R

G

G

A

A

L

I

F

F

G

G

L

L

T

T

R

R

G

G

A

V

N

N

R

A

N

N

H

H

I

I

I

I

R

R

A

A

A

T

L

L

E

E

S

S

I

I

A

A

Y

Y

Q

Q

S

T

K

R

D

D

L

V

L

L

D

E

A

A

M

M

I

Q

K

A

D

D

S

S

G

G

V

I

S

R

L

L

K

H

R

A

L

L

K

R

V

V

D

D

G

G

G
|
G

A
|
A

V

V

A

A

N
|
N

D

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

A

S

D

D

I

I

T

L

D

G

V

T

E

R

V

V

L

E

R

R

P

P

S

E

V

V

C

R

E

E

T

V

T

T

A

A

L

L

G

G

A

A

A

A

F

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

N

Q

S

N

V

L

T

D

E

E

L

L

E

Q

H

E

K

K

A

A

C

V

I

I

D

E

T

R

H

E

F

F

K

R

P

P

S

G

I

I

N

E

A

T

D

T

Y

E

R

R

Q

N

Q

Y

L

R

Y

Y

A

A

G

G

W

W

Q

K

D

K

A

A

V

V

A

K

R

R

binding adenosine-5'-diphosphate: R14 (= R17), G256 (= G265), T257 (= T266), G300 (= G309), I303 (= I312), A316 (= A325), G401 (= G410), A402 (= A411), N405 (= N414)
binding glycerol: R80 (= R83), E81 (= E84), W100 (= W103), Y132 (= Y135), D235 (= D244), F260 (= F269)

P18157 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Bacillus subtilis (strain 168) (see paper)
60% identity, 98% coverage: 5:489/494 of query aligns to 4:489/496 of P18157

query
sites
P18157

Y

Y

V

I

V

L

A

S

L

L

D

D

Q

Q

G

G

T

T

T

T

S

S

S

S

R

R

A

A

I

I

V

L

F

F

D

N

H

K

D

E

A

G

N

K

I

I

V

V

S

H

V

S

S

A

Q

Q

R

K

E

E

F

F

T

T

Q

Q

L

Y

Y

F

P

P

N

H

P

P

G

G

W

W

V

V

E

E

H

H

D

N

P

A

M

N

E

E

I

I

W

W

A

G

S

S

Q

V

S

L

S

A

V

V

L

I

I

A

E

S

S

V

L

I

A

S

R

E

A

S

G

G

I

I

H

S

S

A

D

S

A

Q

V

I

A

A

A

G

I

I

G

G

I

I

T

T

N

N

Q

Q

R

R

E

E

T

T

T

T

I

V

I

V

W

W

E

D

K

K

A

D

T

T

G

G

K

S

P

P

V

V

Y

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

C

S

R

R

R

Q

S

T

A

S

E

G

I

I

C

C

E

E

Q

E

L

L

K

R

A

E

Q

K

G

G

L

Y

E

N

D

D

Y

K

V

F

R

R

Q

E

N

K

T

T

G

G

L

L

L

L

L

I

D

D

P

P

Y

Y

F

F

S

S

G

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

N

V

V

P

E

D

G

A

A

R

R

A

E

K

K

A

A

K

E

R

K

G

G

E

E

L

L

L

L

F

F

G

G

T

T

V

I

D

D

T

T

W

W

L

L

L

I

W

W

K

K

L

M

T

S

E

G

G

G

K

K

V

A

H

H

V

V

T

T

D

D

P

Y

T

S

N

N

A

A

A

S

R

R

T

T

L

L

L

M

F

F

N

N

I

I

H

Y

S

D

L

L

S

K

W

W

D

D

S

D

K

E

L

L

L

L

E

D

A

I

L

L

D

G

I

V

P

P

A

K

A

S

M

M

L

L

P

P

E

E

V

V

K

K

P

P

S

S

C

S

S

H

V

V

Y

Y

G

A

-

E

T

T

T

V

R

D

I

Y

A
x
H

G

F

E
x
F

G

G

S

K

E

N

I

I

P

P

L

I

A

A

G

G

I

A

A

A

G

G

D

D

Q

Q

Q

Q

A

S

A

A

L

L

F

F

G

G

Q

Q

L

A

C

C

V

F

E

E

Q

E

G

G

M

M

A

G

K

K

N

N

T

T

Y

Y

G

G

T

T

G

G

C

C

F

F

L

M

L

L

M

M

N

N

T

T

G

G

S

E

K

K

A

A

V

I

R

K

S

S

S

E

H

H

G

G

L

L

L

L

T

T

T

T

V

I

A

A

V

W

G

G

A

I

Q

D

G

G

E

K

V

V

N

N

Y

Y

A

A

L

L

E

E

G

G

S

S

V

I

F

F

M

V

G

A

G

G

A

S

T

A

I

I

Q

Q

W

W

L

L

R

R

D

D

E

G

L

L

G

R

L

M

I

F

R

Q

D

D

A

S

S

S

D

L

T

S

E

E

Y

S

F

Y

A

A

S

E

K

K

V

V

A

D

D

S

T

T

N

D

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

V

G

G

L

L

G

G

A

T

P

P

Y

Y

W

W

D

D

P

S

N

D

A

V

R

R

G

G

A

S

L

V

F

F

G

G

L

L

T

T

R

R

G

G

A

T

N

T

R

K

N

E

H

H

I

F

I

I

R

R

A

A

A

T

L

L

E

E

S

S

I

L

A

A

Y

Y

Q

Q

S

T

K

K

D

D

L

V

L

L

D

D

A

A

M

M

I

E

K

A

D

D

S

S

G

N

V

I

S

S

L

L

K

K

R

T

L

L

K

R

V

V

D

D

G

G

G

G

A

A

V

V

A

K

N

N

D

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

A

G

D

D

I

L

T

L

D

N

V

V

E

P

V

V

L

E

R

R

P

P

S

E

V

I

C

N

E

E

T

T

T

T

A

A

L

L

G

G

A

A

A

A

F

Y

L

L

A

A

G

G

L

I

A

A

V

V

G

G

F

F

W

W

N

K

S

D

V

R

T

S

E

E

L

I

E

A

H

N

K

Q

A

W

C

N

I

L

D

D

T

K

H

R

F

F

K

E

P

P

S

E

I

L

N

E

A

E

D

E

Y

K

R

R

Q

N

Q

E

L

L

Y

Y

A

K

G

G

W

W

Q

Q

D

K

A

A

V

V

H230 (≠ A230) mutation to R: Increased activity.
F232 (≠ E232) mutation to S: Increased activity.

3ge1A 2.7 angstrom crystal structure of glycerol kinase (glpk) from staphylococcus aureus in complex with adp and glycerol
59% identity, 99% coverage: 3:493/494 of query aligns to 3:494/499 of 3ge1A

query
sites
3ge1A

K

E

K

K

Y

Y

V

I

V

L

A

S

L

I

D

D

Q

Q

G

G

T

T

T

T

S

S

S

S

R

R

A

A

I

I

V

L

F

F

D

N

H

Q

D

K

A

G

N

E

I

I

V

A

S

G

V

V

S

A

Q

Q

R

R

E

E

F

F

T

K

Q

Q

L

Y

Y

F

P

P

N

Q

P

S

G

G

W

W

V

V

E

E

H

H

D

D

P

A

M

N

E

E

I

I

W

W

A

T

S

S

Q

V

S

L

S

A

V

V

L

M

I

T

E

E

S

V

L

I

A

N

R

E

A

N

G

D

I

V

H

R

S

A

D

D

A

Q

V

I

A

A

A

G

I

I

G

G

I

I

T

T

N

N

Q

Q

R

R

E

E

T

T

T

T

I

V

I

V

W

W

E

D

K

K

A

H

T

T

G

G

K

R

P

P

V

I

Y

Y

N

H

A

A

I

I

V

V

W

W

Q

Q

C

S

R

R

R

Q

S

T

A

Q

E

S

I

I

C

C

E

S

Q

E

L

L

K

K

A

Q

Q

Q

G

G

L

Y

E

E

D

Q

Y

T

V

F

R

R

Q

D

N

K

T

T

G

G

L

L

L

L

L

L

D

D

P

P

Y

Y

F

F

S

A

G

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

N

V

V

P

E

D

G

A

A

R

R

A

E

K

K

A

A

K

E

R

N

G

G

E

D

L

L

L

L

F

F

G

G

T

T

V

I

D

D

T

T

W

W

L

L

L

V

W

W

K

K

L

L

T

S

E

G

G

K

K

A

V

A

H

H

V

I

T

T

D

D

P

Y

T

S

N

N

A

A

A

S

R

R

T

T

L

L

L

M

F

F

N

N

I

I

H

H

S

D

L

L

S

E

W

W

D

D

S

D

K

E

L

L

L

L

E

E

A

L

L

L

D

T

I

V

P

P

A

K

A

N

M

M

L

L

P

P

E

E

V

V

K

K

P

A

S

S

C

S

S

E

V

V

Y

Y

G

G

T

K

T

T

-

I

R

D

I

Y

A

H

G

F

E

Y

G

G

S

Q

E

E

I

V

P

P

L

I

A

A

G

G

I

V

A

A

G

G

D

D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

L

A

C

C

V

F

E

E

Q

R

G

G

M

D

A

V

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

G

F

F

L

M

L

L

M

M

N

N

T

T

G

G

S

D

K

K

A

A

V

V

R

K

S

S

S

E

H

S

G

G

L

L

L

L

T

T

T

T

V

I

A

A

V

Y

G

G

A

I

Q

D

G

G

E

K

V

V

N

N

Y

Y

A

A

L

L

E

E

G

G

S

S

V

I

F

F

M

V

G

S

G
|
G

A

S

T

A

I
|
I

Q
|
Q

W

W

L

L

R

R

D

D

E

G

L

L

G

R

L

M

I

I

R

N

D

S

A
|
A

S
x
P

D

Q

T

S

E

E

Y

S

F

Y

A

A

S

T

K

R

V

V

A

D

D

S

T

T

N

E

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

V

G

G

L

L

G

G

A

T

P

P

Y

Y

W

W

D

D

P

S

N

E

A

A

R

R

G

G

A

A

L

I

F

F

G

G

L

L

T

T

R

R

G

G

A

T

N

E

R

K

N

E

H

H

I

F

I

I

R

R

A

A

A

T

L

L

E

E

S

S

I

L

A

C

Y

Y

Q

Q

S

T

K

R

D

D

L

V

L

M

D

E

A

A

M

M

I

S

K

K

D

D

S

S

G

G

V

I

S

D

L

V

K

Q

R

S

L

L

K

R

V

V

D

D

G

G

G
|
G

A
|
A

V

V

A

K

N
|
N

D

N

F

F

L

I

M

M

Q

Q

F

F

Q

Q

A

A

D

D

I

I

T

V

D

N

V

T

E

S

V

V

L

E

R

R

P

P

S

E

V

I

C

Q

E

E

T

T

T

T

A

A

L

L

G

G

A

A

A

A

F

F

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

N

E

S

S

V

K

T

D

E

D

L

I

E

A

H

K

K

N

A

W

C

K

I

L

D

E

T

E

H

K

F

F

K

D

P

P

S

K

I

M

N

D

A

E

D

G

Y

E

R

R

Q

E

Q

K

L

L

Y

Y

A

R

G

G

W

W

Q

K

D

K

A

A

V

V

A

E

R

A

T

T

R

Q

binding adenosine-5'-diphosphate: G266 (= G265), T267 (= T266), G310 (= G309), I313 (= I312), Q314 (= Q313), A326 (= A325), P327 (≠ S326), G411 (= G410), A412 (= A411), N415 (= N414)

Q5HGD2 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Staphylococcus aureus (strain COL)
59% identity, 99% coverage: 3:493/494 of query aligns to 2:493/498 of Q5HGD2

query
sites
Q5HGD2

K

E

K

K

Y

Y

V

I

V

L

A

S

L

I

D

D

Q

Q

G

G

T
|
T

T

T

S

S

S

S

R
|
R

A

A

I

I

V

L

F

F

D

N

H

Q

D

K

A

G

N

E

I

I

V

A

S

G

V

V

S

A

Q

Q

R

R

E

E

F

F

T

K

Q

Q

L

Y

Y

F

P

P

N

Q

P

S

G

G

W

W

V

V

E

E

H

H

D

D

P

A

M

N

E

E

I

I

W

W

A

T

S

S

Q

V

S

L

S

A

V

V

L

M

I

T

E

E

S

V

L

I

A

N

R

E

A

N

G

D

I

V

H

R

S

A

D

D

A

Q

V

I

A

A

A

G

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

V

I

V

W

W

E

D

K

K

A

H

T

T

G

G

K

R

P

P

V

I

Y

Y

N

H

A

A

I

I

V

V

W

W

Q

Q

C

S

R

R

R

Q

S

T

A

Q

E

S

I

I

C

C

E

S

Q

E

L

L

K

K

A

Q

Q

Q

G

G

L

Y

E

E

D

Q

Y

T

V

F

R

R

Q

D

N

K

T

T

G

G

L

L

L

L

L

L

D

D

P

P

Y
|
Y

F

F

S

A

G

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

N

V

V

P

E

D

G

A

A

R

R

A

E

K

K

A

A

K

E

R

N

G

G

E

D

L

L

L

L

F

F

G

G

T

T

V

I

D

D

T

T

W

W

L

L

L

V

W

W

K

K

L

L

T

S

E

G

G

K

K

A

V

A

H

H

V

I

T

T

D

D

P

Y

T

S

N

N

A

A

A

S

R

R

T

T

L

L

L

M

F

F

N

N

I

I

H

H

S

D

L

L

S

E

W

W

D

D

S

D

K

E

L

L

L

L

E

E

A

L

L

L

D

T

I

V

P

P

A

K

A

N

M

M

L

L

P

P

E

E

V

V

K

K

P

A

S

S

C

S

S

E

V

V

Y

Y

G

G

T

K

T

T

-

I

R

D

I

Y

A

H

G

F

E

Y

G

G

S

Q

E

E

I

V

P

P

L

I

A

A

G

G

I

V

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

L

A

C

C

V

F

E

E

Q

R

G

G

M

D

A

V

K

K

N

N

T

T

Y

Y

G

G

T
|
T

G

G

C

G

F

F

L

M

L

L

M

M

N

N

T

T

G

G

S

D

K

K

A

A

V

V

R

K

S

S

S

E

H

S

G

G

L

L

L

L

T

T

T

T

V

I

A

A

V

Y

G

G

A

I

Q

D

G

G

E

K

V

V

N

N

Y

Y

A

A

L

L

E

E

G

G

S

S

V

I

F

F

M

V

G

S

G
|
G

A

S

T

A

I

I

Q
|
Q

W

W

L

L

R

R

D

D

E

G

L

L

G

R

L

M

I

I

R

N

D

S

A

A

S

P

D

Q

T

S

E

E

Y

S

F

Y

A

A

S

T

K

R

V

V

A

D

D

S

T

T

N

E

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

V

G

G

L

L

G

G

A

T

P

P

Y

Y

W

W

D

D

P

S

N

E

A

A

R

R

G

G

A

A

L

I

F

F

G

G

L

L

T

T

R

R

G

G

A

T

N

E

R

K

N

E

H

H

I

F

I

I

R

R

A

A

A

T

L

L

E

E

S

S

I

L

A

C

Y

Y

Q

Q

S

T

K

R

D

D

L

V

L

M

D

E

A

A

M

M

I

S

K

K

D

D

S

S

G

G

V

I

S

D

L

V

K

Q

R

S

L

L

K

R

V

V

D

D

G

G

G
|
G

A

A

V

V

A

K

N
|
N

D

N

F

F

L

I

M

M

Q

Q

F

F

Q

Q

A

A

D

D

I

I

T

V

D

N

V

T

E

S

V

V

L

E

R

R

P

P

S

E

V

I

C

Q

E

E

T

T

T

T

A

A

L

L

G

G

A

A

A

A

F

F

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

N

E

S

S

V

K

T

D

E

D

L

I

E

A

H

K

K

N

A

W

C

K

I

L

D

E

T

E

H

K

F

F

K

D

P

P

S

K

I

M

N

D

A

E

D

G

Y

E

R

R

Q

E

Q

K

L

L

Y

Y

A

R

G

G

W

W

Q

K

D

K

A

A

V

V

A

E

R

A

T

T

R

Q

T12 (= T13) binding
R16 (= R17) binding
R82 (= R83) binding
E83 (= E84) binding
Y134 (= Y135) binding
D244 (= D244) binding
Q245 (= Q245) binding
T266 (= T266) binding
G309 (= G309) binding
Q313 (= Q313) binding
G410 (= G410) binding
N414 (= N414) binding

O34153 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Enterococcus casseliflavus (Enterococcus flavescens) (see 3 papers)
56% identity, 100% coverage: 2:494/494 of query aligns to 3:496/506 of O34153

query
sites
O34153

Q

E

K

K

K

N

Y

Y

V

V

V

M

A

A

L

I

D

D

Q

Q

G

G

T

T

T

T

S

S

S

S

R

R

A

A

I

I

V

I

F

F

D

D

H

R

D

N

A

G

N

K

I

K

V

I

S

G

V

S

S

S

Q

Q

R

K

E

E

F

F

T

P

Q

Q

L

Y

Y

F

P

P

N

K

P

S

G

G

W

W

V

V

E

E

H

H

D

N

P

A

M

N

E

E

I

I

W

W

A

N

S

S

Q

V

S

Q

S

S

V

V

L

I

I

A

E

G

S

A

L

F

A

I

R

E

A

S

G

G

I

I

H

R

S

P

D

E

A

A

V

I

A

A

A

G

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

V

I

V

W

W

E

D

K

K

A

T

T

T

G

G

K

Q

P

P

V

I

Y

A

N

N

A

A

I

I

V

V

W

W

Q

Q

C

S

R

R

R

Q

S

S

A

S

E

P

I

I

C

A

E

D

Q

Q

L

L

K

K

A

V

Q

D

G

G

L

H

E

T

D

E

Y

M

V

I

R

H

Q

E

N

K

T

T

G

G

L

L

L

V

L

I

D

D

P

A

Y
|
Y

F

F

S

S

G

A

T

T

K

K

I

V

K

R

W

W

I

L

L

L

D

D

N

N

V

I

P

E

D

G

A

A

R

Q

A

E

K

K

A

A

K

D

R

N

G

G

E

E

L

L

L

L

F

F

G

G

T

T

V

I

D

D

T

S

W

W

L

L

L

V

W

W

K

K

L

L

T

T

E

D

G

G

K

Q

V

V

H

H

V

V

T

T

D

D

P

Y

T

S

N

N

A

A

A

S

R

R

T

T

L

M

L

L

F

Y

N

N

I

I

H

H

S

K

L

L

S

E

W

W

D

D

S

Q

K

E

L

I

L

L

E

D

A

L

L

L

D

N

I

I

P

P

A

S

A

S

M

M

L

L

P

P

E

E

V

V

K

K

P

S

S

N

C

S

S

E

V

V

Y

Y

G

G

T

H

T

T

R

R

-

S

I

Y

A
x
H

G

F

E

Y

G

G

S

S

E

E

I

V

P

P

L

I

A

A

G

G

I

M

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

L

M

C

A

V

F

E

E

Q

K

G

G

M

M

A

I

K

K

N

N

T

T

Y

Y

G

G

T

T

G

G

C

A

F

F

L

I

L

V

M

M

N

N

T

T

G

G

S

E

K

E

A

P

V

Q

R

L

S

S

S

D

H

N

G

D

L

L

L

L

T

T

T

T

V

I

A

G

V

Y

G

G

A

I

Q

N

G

G

E

K

V

V

N

Y

Y

Y

A

A

L

L

E

E

G

G

S

S

V

I

F

F

M

V

G

A

G

G

A

S

T

A

I

I

Q

Q

W

W

L

L

R

R

D

D

E

G

L

L

G

R

L

M

I

I

R

E

D

T

A

S

S

P

D

Q

T

S

E

E

Y

E

F

L

A

A

S

A

K

K

V

A

A

K

D

G

T

D

N

N

G

E

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

S

N

E

A

A

R

R

G

G

A

A

L

V

F

F

G

G

L

L

T

T

R

R

G

G

A

T

N

T

R

K

N

E

H

D

I

F

I

V

R

R

A

A

A

T

L

L

E

Q

S

A

I

V

A

A

Y

Y

Q

Q

S

S

K

K

D

D

L

V

L

I

D

D

A

T

M

M

I

K

K

K

D

D

S

S

G

G

V

I

S

D

L

I

K

P

R

L

L

L

K

K

V

V

D

D

G

G

G

G

A

A

V

A

A

K

N

N

D

D

F

L

L

L

M

M

Q

Q

F

F

Q

Q

A

A

D

D

I

I

T

L

D

D

V

I

E

D

V

V

L

Q

R

R

P

A

S

A

V

N

C

L

E

E

T

T

T

T

A

A

L

L

G

G

A

A

A

A

F

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

N

K

S

D

V

L

T

D

E

E

L

L

E

K

H

S

K

M

A

A

C

E

I

E

D

G

T

Q

H

M

F

F

K

T

P

P

S

E

I

M

N

P

A

A

D

E

Y

E

R

R

Q

D

Q

N

L

L

Y

Y

A

E

G

G

W

W

Q

K

D

Q

A

A

V

V

A

A

R

A

T

T

R

Q

S

T

R84 (= R83) binding
E85 (= E84) binding
Y136 (= Y135) binding
H232 (≠ A230) modified: Phosphohistidine; by HPr; mutation to A: Loss of phosphorylation, no effect on activity.; mutation to E: Loss of phosphorylation, 2.5-fold reduced activity.; mutation to R: Loss of phosphorylation, 3.4-fold increased activity.
D246 (= D244) binding
Q247 (= Q245) binding

3h3nX Glycerol kinase h232r with glycerol (see paper)
56% identity, 100% coverage: 2:494/494 of query aligns to 2:495/501 of 3h3nX

query
sites
3h3nX

Q

E

K

K

K

N

Y

Y

V

V

V

M

A

A

L

I

D

D

Q

Q

G

G

T
|
T

T

T

S

S

S

S

R

R

A

A

I

I

V

I

F

F

D

D

H

R

D

N

A

G

N

K

I

K

V

I

S

G

V

S

S

S

Q

Q

R

K

E

E

F

F

T

P

Q

Q

L

Y

Y

F

P

P

N

K

P

S

G

G

W

W

V

V

E

E

H

H

D

N

P

A

M

N

E

E

I

I

W

W

A

N

S

S

Q

V

S

Q

S

S

V

V

L

I

I

A

E

G

S

A

L

F

A

I

R

E

A

S

G

G

I

I

H

R

S

P

D

E

A

A

V

I

A

A

A

G

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

V

I

V

W

W

E

D

K

K

A

T

T

T

G

G

K

Q

P

P

V

I

Y

A

N

N

A

A

I

I

V

V

W
|
W

Q

Q

C

S

R

R

R

Q

S

S

A

S

E

P

I

I

C

A

E

D

Q

Q

L

L

K

K

A

V

Q

D

G

G

L

H

E

T

D

E

Y

M

V

I

R

H

Q

E

N

K

T

T

G

G

L

L

L

V

L

I

D

D

P

A

Y
|
Y

F

F

S

S

G

A

T

T

K

K

I

V

K

R

W

W

I

L

L

L

D

D

N

N

V

I

P

E

D

G

A

A

R

Q

A

E

K

K

A

A

K

D

R

N

G

G

E

E

L

L

L

L

F

F

G

G

T

T

V

I

D

D

T

S

W

W

L

L

L

V

W

W

K

K

L

L

T

T

E

D

G

G

K

Q

V

V

H

H

V

V

T

T

D

D

P

Y

T

S

N

N

A

A

A

S

R

R

T

T

L

M

L

L

F

Y

N

N

I

I

H

H

S

K

L

L

S

E

W

W

D

D

S

Q

K

E

L

I

L

L

E

D

A

L

L

L

D

N

I

I

P

P

A

S

A

S

M

M

L

L

P

P

E

E

V

V

K

K

P

S

S

N

C

S

S

E

V

V

Y

Y

G

G

T

H

T

T

R

R

-

S

I

Y

A

R

G

F

E

Y

G

G

S

S

E

E

I

V

P

P

L

I

A

A

G

G

I

M

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

L

M

C

A

V

F

E

E

Q

K

G

G

M

M

A

I

K

K

N

N

T

T

Y

Y

G

G

T
|
T

G

G

C

A

F
|
F

L

I

L

V

M

M

N

N

T

T

G

G

S

E

K

E

A

P

V

Q

R

L

S

S

S

D

H

N

G

D

L

L

L

L

T

T

T

T

V

I

A

G

V

Y

G

G

A

I

Q

N

G

G

E

K

V

V

N

Y

Y

Y

A

A

L

L

E

E

G

G

S

S

V

I

F

F

M

V

G

A

G

G

A

S

T

A

I

I

Q

Q

W

W

L

L

R

R

D

D

E

G

L

L

G

R

L

M

I

I

R

E

D

T

A

S

S

P

D

Q

T

S

E

E

Y

E

F

L

A

A

S

A

K

K

V

A

A

K

D

G

T

D

N

N

G

E

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

S

N

E

A

A

R

R

G

G

A

A

L

V

F

F

G

G

L

L

T

T

R

R

G

G

A

T

N

T

R

K

N

E

H

D

I

F

I

V

R

R

A

A

A

T

L

L

E

Q

S

A

I

V

A

A

Y

Y

Q

Q

S

S

K

K

D

D

L

V

L

I

D

D

A

T

M

M

I

K

K

K

D

D

S

S

G

G

V

I

S

D

L

I

K

P

R

L

L

L

K

K

V

V

D

D

G

G

G

G

A

A

V

A

A

K

N

N

D

D

F

L

L

L

M

M

Q

Q

F

F

Q

Q

A

A

D

D

I

I

T

L

D

D

V

I

E

D

V

V

L

Q

R

R

P

A

S

A

V

N

C

L

E

E

T

T

T

T

A

A

L

L

G

G

A

A

A

A

F

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

N

K

S

D

V

L

T

D

E

E

L

L

E

K

H

S

K

M

A

A

C

E

I

E

D

G

T

Q

H

M

F

F

K

T

P

P

S

E

I

M

N

P

A

A

D

E

Y

E

R

R

Q

D

Q

N

L

L

Y

Y

A

E

G

G

W

W

Q

K

D

Q

A

A

V

V

A

A

R

A

T

T

R

Q

S

T

binding glycerol: R83 (= R83), E84 (= E84), W103 (= W103), Y135 (= Y135), D245 (= D244), F270 (= F269)
binding phosphate ion: T13 (= T13), T267 (= T266)

6udeB Crystal structure of glycerol kinase from elizabethkingia anophelis nuhp1 in complex with adp and glycerol
57% identity, 100% coverage: 3:494/494 of query aligns to 2:491/495 of 6udeB

query
sites
6udeB

K

E

K

K

Y

L

V

I

V

L

A

A

L

L

D

D

Q

Q

G
|
G

T
|
T

T
|
T

S
|
S

S

S

R
|
R

A

A

I

I

V

L

F

F

D

N

H

K

D

S

A

G

N

E

I

I

V

K

S

F

V

V

S

S

Q

Q

R

K

E

S

F

F

T

E

Q

Q

L

I

Y

F

P

P

N

T

P

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

M

N

E

E

I

I

W

W

A

S

S

S

Q

Q

S

I

S

S

V

V

L

A

I

A

E

E

S

V

L

I

A

A

R

K

A

A

G

G

I

I

H

S

S

G

D

L

A

E

V

V

A

A

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R

R

E

E

T

T

T

T

I

V

I

V

W

W

E

D

K

R

A

H

T

T

G

S

K

E

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

C

D

R

R

R

R

S

T

A

S

E

K

I

Y

C

C

E

D

Q

E

L

L

K

K

A

S

Q

Q

G

G

L

H

E

T

D

D

Y

E

V

I

R

K

Q

Q

N

K

T

T

G

G

L

L

L

V

L

L

D

D

P

A

Y

Y

F

F

S

S

G

A

T

T

K

K

I

L

K

K

W

W

I

I

L

L

D

D

N

N

V

V

P

E

D

G

A

A

R

R

A

E

K

K

A

A

K

E

R

A

G

G

E

D

L

L

L

C

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

L

I

W

W

K

K

L

L

T

T

E

R

G

G

K

K

V

M

H

F

V

I

T

T

D

D

P

V

T

S

N

N

A

A

A

S

R

R

T

T

L

M

L

M

F

F

N

N

I

I

H

R

S

T

L

M

S

D

W

W

D

D

S

D

K

D

L

L

L

L

E

K

A

L

L

F

D

N

I

I

P

P

A

R

A

A

M

I

L

L

P

P

E

E

V

V

K

K

P

Q

S

S

C

S

S

E

V

V

Y

Y

G

G

T

E

T

T

R

S

I

T

A

T

G

-

E

-

G

S

S

T

E

K

I

I

P

P

L

I

A

A

G

G

I

I

A

A

G

G

D

D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

L

M

C

C

V

T

E

K

Q

P

G

G

M

M

A

V

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

C

F

F

L

L

L

L

M

M

N

N

T

T

G

G

S

N

K

E

A

A

V

V

R

Y

S

S

S

K

H

N

G

N

L

L

L

L

T

T

T

T

V

V

A

A

V

W

G

K

A

I

Q

N

G

G

E

E

V

V

N

S

Y

Y

A

A

L

L

E

E

G

G

S

S

V

V

F

F

M

V

G

G

G
|
G

A

A

T

A

I
|
I

Q

Q

W

W

L

L

R

R

D

D

E

G

L

L

G

K

L

I

I

I

R

H

D

D

A

S

S
|
S

D

E

T

V

E

S

Y

T

F

L

A

A

S

E

K

T

V

V

A

E

D

D

T

N

N

G

G

G

V

V

Y

Y

L

F

V

V

P

P

A

A

F

L

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

Q

N

Y

A

A

R

R

G

G

A

T

L

I

F

I

G

G

L

V

T

T

R

R

G

G

A

T

N

T

R

D

N

G

H

H

I

I

I

A

R

R

A

A

A

T

L

L

E

E

S

G

I

I

A

A

Y

F

Q

Q

S

V

K

Y

D

D

L

I

L

V

D

K

A

A

M

M

I

E

K

A

D

D

S

A

G

E

V

T

S

Q

L

S

K

T

R

E

L

L

K

R

V

V

D

D

G
|
G

G
|
G

A
|
A

V

S

A

A

N

S

D

N

F

L

L

L

M

M

Q

Q

F

I

Q

Q

A

S

D

D

I

L

T

F

D

G

V

F

E

K

V

I

L

I

R

R

P

P

S

K

V

T

C

L

E

E

T

T

T

T

A

A

L

L

G

G

A

A

A

A

F

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

N

E

S

S

V

I

T

D

E

E

L

I

E

Q

H

S

K

Q

A

W

C

I

I

I

D

E

T

K

H

E

F

F

K

T

P

P

S

K

I

E

N

D

A

K

D

T

Y

K

R

I

Q

D

Q

N

L

M

Y

V

A

S

G

F

W

W

Q

H

D

K

A

A

V

V

A

K

R

R

T

S

R

Q

S

A

binding adenosine-5'-diphosphate: R16 (= R17), G262 (= G265), T263 (= T266), G306 (= G309), I309 (= I312), S323 (= S326), G406 (= G409), G407 (= G410), A408 (= A411)
binding magnesium ion: G11 (= G12), T12 (= T13), T13 (= T14), S14 (= S15)

O34154 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Enterococcus faecalis (strain ATCC 700802 / V583) (see 2 papers)
55% identity, 100% coverage: 2:493/494 of query aligns to 3:494/501 of O34154

query
sites
O34154

Q

E

K

E

K

K

Y

Y

V

I

V

M

A

A

L

I

D

D

Q

Q

G

G

T

T

T

T

S

S

S

S

R

R

A

A

I

I

V

I

F

F

D

D

H

K

D

K

A

G

N

N

I

K

V

I

S

G

V

S

S

S

Q

Q

R

K

E

E

F

F

T

T

Q

Q

L

Y

Y

F

P

P

N

N

P

A

G

G

W

W

V

V

E

E

H

H

D

N

P

A

M

N

E

E

I

I

W

W

A

N

S

S

Q

V

S

Q

S

S

V

V

L

I

I

A

E

G

S

S

L

L

A

I

R

E

A

S

G

G

I

V

H

K

S

P

D

T

A

D

V

I

A

A

A

G

I

I

G

G

I

I

T

T

N

N

Q

Q

R

R

E

E

T

T

T

T

I

V

I

V

W

W

E

D

K

K

A

A

T

T

G

G

K

L

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

C

S

R

R

R

Q

S

T

A

T

E

P

I

I

C

A

E

D

Q

Q

L

L

K

K

A

E

Q

D

G

G

L

Y

E

S

D

E

Y

M

V

I

R

H

Q

E

N

K

T

T

G

G

L

L

L

I

L

I

D

D

P

A

Y

Y

F

F

S

S

G

A

T

T

K

K

I

V

K

R

W

W

I

I

L

L

D

D

N

H

V

V

P

E

D

G

A

A

R

Q

A

E

K

R

A

A

K

E

R

N

G

G

E

E

L

L

L

M

F

F

G

G

T

T

V

I

D

D

T

T

W

W

L

L

L

V

W

W

K

K

L

L

T

T

E

-

G

G

K

D

V

T

H

H

V

V

T

T

D

D

P

Y

T

S

N

N

A

A

A

S

R

R

T

T

L

M

L

L

F

F

N

N

I

I

H

H

S

D

L

L

S

D

W

W

D

D

S

Q

K

E

L

I

L

L

E

D

A

L

L

L

D

N

I

I

P

P

A

R

A

V

M

M

L

L

P

P

E

K

V

V

K

V

P

S

S

N

C

S

S

E

V

V

Y

Y

G

G

T

L

T

T

R

K

-

N

I

Y

A
x
H

G

F

E

Y

G

G

S

S

E

E

I

V

P

P

L

I

A

A

G

G

I

M

A

A

G

G

D

D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

L

M

C

A

V

F

E

E

Q

P

G

G

M

M

A

V

K

K

N

N

T

T

Y

Y

G

G

T

T

G

G

C

S

F

F

L

I

L

V

M

M

N

N

T

T

G

G

S

E

K

E

A

P

V

Q

R

L

S

S

S

K

H

N

G

N

L

L

L

L

T

T

T

T

V

I

A

G

V

Y

G

G

A

I

Q

N

G

G

E

K

V

V

N

Y

Y

Y

A

A

L

L

E

E

G

G

S

S

V

I

F

F

M

V

G

A

G

G

A

S

T

A

I

I

Q

Q

W

W

L

L

R

R

D

D

E

G

L

L

G

K

L

M

I

L

R

Q

D

T

A

A

S

A

D

E

T

S

E

E

Y

A

F

V

A

A

S

K

K

A

V

S

A

T

D

G

T

H

N

N

G

E

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

S

N

Q

A

A

R

R

G

G

A

A

L

V

F

F

G

G

L

L

T

T

R

R

G

G

A

T

N

T

R

R

N

E

H

D

I

F

I

V

R

K

A

A

A

T

L

L

E

Q

S

A

I

V

A

A

Y

Y

Q

Q

S

V

K

R

D

D

L

I

L

I

D

D

A

T

M

M

I

K

K

E

D

D

S

T

G

G

V

I

S

D

L

I

K

P

R

V

L

L

K

K

V

V

D

D

G

G

G

G

A

A

V

A

A

N

N

N

D

D

F

F

L

L

M

M

Q

Q

F

F

Q

Q

A

A

D

D

I

I

T

L

D

N

V

T

E

A

V

V

L

Q

R

R

P

A

S

H

V

N

C

L

E

E

T

T

T

T

A

A

L

L

G

G

A

A

A

A

F

F

L

L

A

A

G

G

L

L

A

A

V

V

G

G

F

F

W

W

N

K

S

D

V

L

T

E

E

E

L

I

E

K

H

A

K

F

A

Q

C

E

I

E

D

G

T

Q

H

Q

F

F

K

E

P

P

S

I

I

M

N

A

A

E

D

E

Y

E

R

R

Q

E

Q

D

L

L

Y

Y

A

E

G

G

W

W

Q

Q

D

Q

A

A

V

V

A

A

R

A

T

T

R

Q

H231 (≠ A230) modified: Phosphohistidine; by HPr

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6k76A Glycerol kinase form thermococcus kodakarensis, complex structure with substrate.
55% identity, 98% coverage: 6:491/494 of query aligns to 1:476/485 of 6k76A

query
sites
6k76A

V

V

V

L

A

S

L

L

D

D

Q

E

G

G

T
|
T

T

T

S

S

S

A

R

R

A

A

I

I

V

I

F

F

D

D

H

R

D

E

A

S

N

N

I

I

V

H

S

G

V

I

S

G

Q

Q

R

Y

E

E

F

F

T

P

Q

Q

L

H

Y

Y

P

P

N

R

P

P

G

G

W

W

V

V

E

E

H

H

D

N

P

P

M

E

E

E

I

I

W

W

A

D

S

A

Q

Q

S

L

S

R

V

A

L

I

I

K

E

D

S

A

L

I

A

Q

R

S

A

A

G

R

I

-

H

E

S

P

D

N

A

Q

V

I

A

A

A

A

I

I

G

G

I

V

T

T

N

N

Q

Q

R

R

E

E

T

T

T

T

I

L

I

V

W

W

E

D

K

K

A

-

T

D

G

G

K

K

P

P

V

L

Y

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

C

C

R

R

R

R

S

T

A

A

E

E

I

M

C

V

E

E

Q

E

L

I

K

K

A

R

Q

E

G

-

L

Y

E

G

D

T

Y

M

V

I

R

K

Q

E

N

K

T

T

G

G

L

L

L

V

L

P

D

D

P

A

Y

Y

F

F

S

S

G

A

T

S

K

K

I

L

K

K

W

W

I

L

L

L

D

D

N

N

V

V

P

P

D

G

A

L

R

R

A

E

K

K

A

A

K

E

R

K

G

G

E

E

L

V

L

M

F

F

G

G

T

T

V

V

D

D

T

T

W

F

L

L

L

I

W

Y

K

R

L

L

T

T

E

-

G

G

K

E

V

-

H

H

V

V

T

T

D

D

P

Y

T

S

N

N

A

A

A

S

R

R

T

T

L

M

L

L

F

F

N

N

I

I

H

K

S

K

L

L

S

D

W

W

D

D

S

D

K

E

L

L

L

L

E

E

A

L

L

F

D

D

I

I

P

P

A

E

A

S

M

V

L

L

P

P

E

E

V

V

K

R

P

E

S

S

C

S

S

E

V

V

Y

Y

G

G

T

Y

T

T

R

K

I

-

A

-

G

K

E

E

G

L

S

A

E

E

I

I

P

P

L

V

A

S

G

G

I

D

A

A

G

G

D

D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

L

A

C

A

V

F

E

E

Q

A

G

G

M

M

A

V

K

K

N

A

T

T

Y

Y

G

G

T
|
T

G

G

C

S

F

F

L

I

L

L

M

V

N

N

T

T

G

-

S

D

K

K

A

M

V

V

R

L

S

Y

S

S

H

D

G

N

L

L

L

L

T

T

T

T

V

I

A

A

V

W

G

G

A

L

Q

N

G

G

E

R

V

V

N

S

Y

Y

A

A

L

L

E

E

G

G

S

S

V

I

F

F

M

V

G

T

G
|
G

A

A

T

A

I
x
V

Q
|
Q

W

W

L

L

R

R

D

D

E

G

L

I

G

K

L

I

I

I

R

K

D

H

A

A

S

S

D

E

T

T

E

E

Y

E

F

L

A

A

S

T

K

K

V

L

A

E

D

S

T

N

N

E

G

G

V

V

Y

Y

L

F

V

V

P

P

A

A

F

F

V

V

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

P

Q

N

F

A

A

R

R

G

G

A

I

L

I

F

I

G

G

L

I

T

T

R

R

G

G

A

T

N

G

R

R

N

E

H

H

I

L

I

A

R

R

A

A

A

T

L

L

E

E

S

A

I

I

A

A

Y

Y

Q

L

S

T

K

R

D

D

L

V

L

V

D

D

A

E

M

M

I

-

K

-

D

E

S

K

G

L

V

V

S

Q

L

I

K

K

R

E

L

L

K

R

V

V

D

D

G

G

G
|
G

A
|
A

V

T

A

A

N
|
N

D

D

F

F

L

L

M

M

Q

Q

F

F

Q

Q

A

A

D

D

I

I

T

L

D

N

V

R

E

K

V

V

L

I

R

R

P

P

S

V

V

V

C

K

E

E

T

T

T

T

A

A

L

L

G

G

A

A

A

A

F

Y

L

L

A

A

G

G

L

L

A

A

V

V

G

D

F

Y

W

W

N

A

S

D

V

T

T

R

E

E

L

I

E

A

H

E

K

L

A

W

C

K

I

A

D

E

T

R

H

I

F

F

K

E

P

P

S

K

I

M

N

D

A

E

D

K

Y

T

R

R

Q

E

Q

R

L

L

Y

Y

A

K

G

G

W

W

Q

K

D

E

A

A

V

V

A

K

R

R

binding phosphoaminophosphonic acid-adenylate ester: T8 (= T13), T254 (= T266), G296 (= G309), V299 (≠ I312), Q300 (= Q313), G395 (= G410), A396 (= A411), N399 (= N414)

O86033 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
52% identity, 99% coverage: 5:492/494 of query aligns to 4:493/497 of O86033

query
sites
O86033

Y

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V

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R82 (= R83) binding
E83 (= E84) binding
Y134 (= Y135) binding
D243 (= D244) binding
Q244 (= Q245) binding

5gn6A Crystal structure of glycerol kinase from trypanosoma brucei gambiense complexed with cumarin derivative-17b (see paper)
45% identity, 99% coverage: 4:493/494 of query aligns to 4:510/513 of 5gn6A

query
sites
5gn6A

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x
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-

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binding 4-butyl-7,8-bis(oxidanyl)chromen-2-one: Y141 (= Y135), R194 (= R188), F281 (= F269), L298 (= L286), E316 (= E302), A318 (≠ S304)
binding glycerol: R86 (= R83), E87 (= E84), W106 (= W103), D256 (= D244)

Query Sequence

>203312 FitnessBrowser__MR1:203312
MQKKYVVALDQGTTSSRAIVFDHDANIVSVSQREFTQLYPNPGWVEHDPMEIWASQSSVL
IESLARAGIHSDAVAAIGITNQRETTIIWEKATGKPVYNAIVWQCRRSAEICEQLKAQGL
EDYVRQNTGLLLDPYFSGTKIKWILDNVPDARAKAKRGELLFGTVDTWLLWKLTEGKVHV
TDPTNAARTLLFNIHSLSWDSKLLEALDIPAAMLPEVKPSCSVYGTTRIAGEGSEIPLAG
IAGDQQAALFGQLCVEQGMAKNTYGTGCFLLMNTGSKAVRSSHGLLTTVAVGAQGEVNYA
LEGSVFMGGATIQWLRDELGLIRDASDTEYFASKVADTNGVYLVPAFVGLGAPYWDPNAR
GALFGLTRGANRNHIIRAALESIAYQSKDLLDAMIKDSGVSLKRLKVDGGAVANDFLMQF
QADITDVEVLRPSVCETTALGAAFLAGLAVGFWNSVTELEHKACIDTHFKPSINADYRQQ
LYAGWQDAVARTRS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory