SitesBLAST
Comparing 203435 FitnessBrowser__MR1:203435 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P18776 Anaerobic dimethyl sulfoxide reductase chain B; DMSO reductase iron-sulfur subunit from Escherichia coli (strain K12) (see 2 papers)
55% identity, 95% coverage: 5:198/205 of query aligns to 3:194/205 of P18776
- C102 (= C106) mutation C->F,S,W,Y: Loss of electron transfer from menaquinol to DMSO.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
2ivfB Ethylbenzene dehydrogenase from aromatoleum aromaticum (see paper)
41% identity, 56% coverage: 46:159/205 of query aligns to 112:225/337 of 2ivfB
- binding fe3-s4 cluster: C148 (= C82), T150 (= T84), I153 (≠ M87), C169 (= C103), K170 (≠ I104), G171 (= G105), H172 (≠ C106), R173 (≠ E107), H174 (≠ S108), C175 (= C109), S193 (≠ M127)
- binding protoporphyrin ix containing fe: T150 (= T84), K170 (≠ I104), H172 (≠ C106)
- binding iron/sulfur cluster: M135 (≠ S69), C136 (= C70), N137 (= N71), H138 (= H72), C139 (= C73), P142 (= P76), C144 (= C78), V162 (= V96), C179 (= C113), Y181 (= Y115), A183 (= A117), I184 (≠ P118), K195 (= K129), C196 (= C130), I197 (≠ D131), L198 (≠ G132), C199 (= C133), N209 (≠ P143), A210 (≠ S144), C211 (= C145), C215 (= C149), G217 (≠ Q151), V219 (≠ A153), R220 (≠ L154)
Sites not aligning to the query:
- binding iron/sulfur cluster: 13, 14, 15, 16, 17, 18, 19, 23, 27, 38, 39, 41
5ch7B Crystal structure of the perchlorate reductase pcrab - phe164 gate switch intermediate - from azospira suillum ps (see paper)
33% identity, 77% coverage: 48:205/205 of query aligns to 111:258/329 of 5ch7B
- binding fe3-s4 cluster: C145 (= C82), I150 (≠ M87), C166 (= C103), K167 (≠ I104), G168 (= G105), A169 (≠ C106), Q170 (≠ E107), A171 (≠ S108), C172 (= C109), A190 (≠ M127)
- binding iron/sulfur cluster: P130 (≠ S67), M132 (≠ S69), C133 (= C70), N134 (= N71), H135 (= H72), C136 (= C73), P139 (= P76), C141 (= C78), C176 (= C113), P181 (= P118), K192 (= K129), C193 (= C130), I194 (≠ D131), G195 (= G132), C196 (= C133), P206 (= P143), A207 (≠ S144), C208 (= C145), C212 (= C149), V213 (≠ P150), G214 (≠ Q151)
- binding sulfite ion: W112 (= W49)
Sites not aligning to the query:
- binding iron/sulfur cluster: 17, 18, 19, 20, 21, 22, 23, 27, 31, 43, 43, 45
- binding sulfite ion: 29, 33, 106, 107
1kqfB Formate dehydrogenase n from e. Coli (see paper)
31% identity, 88% coverage: 11:190/205 of query aligns to 33:222/289 of 1kqfB
- binding protoporphyrin ix containing fe: Y137 (≠ S108)
- binding iron/sulfur cluster: C38 (= C16), I39 (≠ S17), G40 (= G18), C41 (= C19), K42 (= K20), A43 (≠ T21), C44 (= C22), C48 (= C26), N52 (≠ S30), T77 (= T50), M79 (≠ D52), K96 (≠ S67), C99 (= C70), M100 (≠ N71), H101 (= H72), C102 (= C73), P105 (= P76), G106 (≠ V77), C107 (= C78), C111 (= C82), P112 (= P83), A116 (≠ M87), I117 (≠ Y88), V125 (= V96), C132 (= C103), I133 (= I104), G134 (= G105), C135 (= C106), G136 (≠ E107), Y137 (≠ S108), C138 (= C109), C142 (= C113), F144 (≠ Y115), I146 (≠ A117), P147 (= P118), V156 (≠ M127), K158 (= K129), C159 (= C130), T160 (≠ D131), L161 (≠ G132), C162 (= C133), P172 (= P143), A173 (≠ S144), C174 (= C145), C178 (= C149), P179 (= P150), I183 (≠ L154)
Sites not aligning to the query:
P0AAJ3 Formate dehydrogenase, nitrate-inducible, iron-sulfur subunit; Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta from Escherichia coli (strain K12) (see paper)
31% identity, 88% coverage: 11:190/205 of query aligns to 34:223/294 of P0AAJ3
- C39 (= C16) binding
- C42 (= C19) binding
- C45 (= C22) binding
- C49 (= C26) binding
- C100 (= C70) binding
- C103 (= C73) binding
- C108 (= C78) binding
- C112 (= C82) binding
- C133 (= C103) binding
- C136 (= C106) binding
- C139 (= C109) binding
- C143 (= C113) binding
- C160 (= C130) binding
- C163 (= C133) binding
- C175 (= C145) binding
- C179 (= C149) binding
2vpyF Polysulfide reductase with bound quinone inhibitor, pentachlorophenol (pcp) (see paper)
36% identity, 74% coverage: 6:157/205 of query aligns to 3:143/193 of 2vpyF
- binding pentachlorophenol: I91 (= I104), C93 (= C106)
- binding iron/sulfur cluster: C13 (= C16), V14 (≠ S17), G15 (= G18), C16 (= C19), A17 (≠ K20), A18 (≠ T21), C19 (= C22), C23 (= C26), N27 (≠ S30), L36 (≠ N48), I38 (≠ T50), P55 (≠ S67), Q57 (≠ S69), C58 (= C70), L59 (≠ N71), H60 (= H72), C61 (= C73), P64 (= P76), P65 (≠ V77), C66 (= C78), C70 (= C82), P71 (= P83), T72 (= T84), S75 (≠ M87), V83 (= V96), C90 (= C103), I91 (= I104), A92 (≠ G105), C93 (= C106), G94 (≠ E107), A95 (≠ S108), C96 (= C109), C100 (= C113), P101 (= P114), Y102 (= Y115), R105 (≠ P118), V113 (= V126), C116 (= C130), T117 (≠ D131), F118 (≠ G132), C119 (= C133), P129 (= P143), A130 (≠ S144), C131 (= C145), C135 (= C149), T137 (≠ Q151), C139 (≠ A153), R140 (≠ L154)
Sites not aligning to the query:
2vpxB Polysulfide reductase with bound quinone (uq1) (see paper)
36% identity, 74% coverage: 6:157/205 of query aligns to 3:143/193 of 2vpxB
- binding iron/sulfur cluster: C13 (= C16), V14 (≠ S17), G15 (= G18), C16 (= C19), A18 (≠ T21), C19 (= C22), C23 (= C26), N27 (≠ S30), N35 (≠ G47), L36 (≠ N48), I38 (≠ T50), P55 (≠ S67), Q57 (≠ S69), C58 (= C70), L59 (≠ N71), H60 (= H72), C61 (= C73), P64 (= P76), C66 (= C78), C70 (= C82), P71 (= P83), T72 (= T84), A74 (= A86), S75 (≠ M87), V83 (= V96), C90 (= C103), I91 (= I104), A92 (≠ G105), C93 (= C106), G94 (≠ E107), A95 (≠ S108), C96 (= C109), C100 (= C113), Y102 (= Y115), R105 (≠ P118), R105 (≠ P118), V113 (= V126), K115 (= K129), C116 (= C130), T117 (≠ D131), F118 (≠ G132), C119 (= C133), P129 (= P143), A130 (≠ S144), C131 (= C145), C135 (= C149), T137 (≠ Q151), C139 (≠ A153), R140 (≠ L154)
- binding ubiquinone-1: I91 (= I104), C93 (= C106)
2vpwF Polysulfide reductase with bound menaquinone (see paper)
36% identity, 74% coverage: 6:157/205 of query aligns to 3:143/193 of 2vpwF
- binding menaquinone-7: I91 (= I104), C93 (= C106)
- binding iron/sulfur cluster: C13 (= C16), V14 (≠ S17), G15 (= G18), C16 (= C19), A17 (≠ K20), A18 (≠ T21), C19 (= C22), C23 (= C26), N27 (≠ S30), L36 (≠ N48), I38 (≠ T50), P55 (≠ S67), Q57 (≠ S69), C58 (= C70), L59 (≠ N71), H60 (= H72), C61 (= C73), P64 (= P76), P65 (≠ V77), C66 (= C78), C70 (= C82), P71 (= P83), T72 (= T84), S75 (≠ M87), V83 (= V96), C90 (= C103), I91 (= I104), A92 (≠ G105), C93 (= C106), G94 (≠ E107), A95 (≠ S108), C96 (= C109), C100 (= C113), P101 (= P114), Y102 (= Y115), R105 (≠ P118), R105 (≠ P118), V113 (= V126), C116 (= C130), T117 (≠ D131), F118 (≠ G132), C119 (= C133), P129 (= P143), A130 (≠ S144), C131 (= C145), C135 (= C149), T137 (≠ Q151), C139 (≠ A153), R140 (≠ L154)
Sites not aligning to the query:
6cz7B The arsenate respiratory reductase (arr) complex from shewanella sp. Ana-3 (see paper)
30% identity, 93% coverage: 6:196/205 of query aligns to 2:221/234 of 6cz7B
- binding iron/sulfur cluster: C12 (= C16), V13 (≠ S17), G14 (= G18), C15 (= C19), G16 (≠ K20), G17 (≠ T21), C18 (= C22), C22 (= C26), N26 (≠ S30), W34 (= W38), S35 (≠ G47), Y52 (= Y65), P54 (≠ S67), L56 (≠ S69), C57 (= C70), N58 (= N71), H59 (= H72), C60 (= C73), A63 (≠ P76), P64 (≠ V77), C65 (= C78), C69 (= C82), P70 (= P83), T71 (= T84), M74 (= M87), T82 (≠ V96), C89 (= C103), I90 (= I104), G91 (= G105), C92 (= C106), K93 (≠ E107), K94 (≠ S108), C95 (= C109), C99 (= C113), Y101 (= Y115), V103 (≠ A117), I104 (vs. gap), T161 (≠ M127), K163 (= K129), C164 (= C130), F166 (≠ G132), C167 (= C133), A178 (≠ S144), C179 (= C145), C183 (= C149), P184 (= P150), R188 (≠ L154)
Q7WTT9 Arsenate respiratory reductase iron-sulfur subunit ArrB; Arsenate respiratory reductase small subunit; ARR small subunit from Shewanella sp. (strain ANA-3) (see paper)
30% identity, 93% coverage: 6:196/205 of query aligns to 2:221/234 of Q7WTT9
- C12 (= C16) binding
- C15 (= C19) binding
- C18 (= C22) binding
- C22 (= C26) binding
- C57 (= C70) binding
- C60 (= C73) binding
- C65 (= C78) binding
- C69 (= C82) binding
- C89 (= C103) binding
- C92 (= C106) binding
- C95 (= C109) binding
- C99 (= C113) binding
- C164 (= C130) binding
- C167 (= C133) binding
- C179 (= C145) binding
- C183 (= C149) binding
8bqgB W-formate dehydrogenase from desulfovibrio vulgaris - soaking with formate 1 min (see paper)
30% identity, 89% coverage: 9:190/205 of query aligns to 4:199/214 of 8bqgB
- binding iron/sulfur cluster: F4 (= F9), C11 (= C16), T12 (≠ S17), A13 (≠ G18), C14 (= C19), R15 (≠ K20), G16 (≠ T21), C17 (= C22), C21 (= C26), K25 (≠ S30), K50 (vs. gap), Q72 (≠ S69), C73 (= C70), R74 (≠ N71), H75 (= H72), C76 (= C73), P80 (≠ V77), C81 (= C78), V102 (= V96), C120 (= C113), Y122 (= Y115), I124 (≠ A117), P125 (= P118), K136 (= K129), C137 (= C130), D138 (= D131), M139 (≠ G132), C140 (= C133), P150 (= P143), A151 (≠ S144), C152 (= C145), C156 (= C149), P157 (= P150), T158 (≠ Q151), T160 (≠ A153), M161 (≠ L154)
1h0hB Tungsten containing formate dehydrogenase from desulfovibrio gigas (see paper)
32% identity, 76% coverage: 8:163/205 of query aligns to 3:170/214 of 1h0hB
- binding iron/sulfur cluster: F4 (= F9), C11 (= C16), T12 (≠ S17), A13 (≠ G18), C14 (= C19), R15 (≠ K20), G16 (≠ T21), C17 (= C22), C21 (= C26), H25 (≠ R39), K50 (vs. gap), P69 (≠ S67), Q71 (≠ S69), C72 (= C70), R73 (≠ N71), H74 (= H72), C75 (= C73), P79 (≠ V77), C80 (= C78), V101 (= V96), C120 (= C113), Y122 (= Y115), V124 (≠ A117), P125 (= P118), K136 (= K129), C137 (= C130), D138 (= D131), M139 (≠ G132), C140 (= C133), A151 (≠ S144), C152 (= C145), C156 (= C149), P157 (= P150), A160 (= A153), M161 (≠ L154)
Q8GC87 Formate dehydrogenase subunit beta; FDH subunit beta; Formate dehydrogenase small subunit from Megalodesulfovibrio gigas (strain ATCC 19364 / DSM 1382 / NCIMB 9332 / VKM B-1759) (Desulfovibrio gigas) (see paper)
32% identity, 76% coverage: 8:163/205 of query aligns to 4:171/215 of Q8GC87
- C12 (= C16) binding
- C15 (= C19) binding
- C18 (= C22) binding
- C22 (= C26) binding
- C73 (= C70) binding
- C76 (= C73) binding
- C81 (= C78) binding
- C121 (= C113) binding
- C138 (= C130) binding
- C141 (= C133) binding
- C153 (= C145) binding
- C157 (= C149) binding
7qv7B Cryo-em structure of hydrogen-dependent co2 reductase. (see paper)
29% identity, 83% coverage: 5:175/205 of query aligns to 1:180/183 of 7qv7B
- binding iron/sulfur cluster: C14 (= C16), I15 (≠ S17), G16 (= G18), C17 (= C19), K18 (= K20), A19 (≠ T21), C20 (= C22), C24 (= C26), H28 (≠ K27), R48 (≠ A56), C63 (= C70), R64 (≠ N71), H65 (= H72), C66 (= C73), A69 (≠ P76), C71 (= C78), C75 (= C82), A79 (= A86), I80 (≠ M87), C94 (= C103), I95 (= I104), C97 (= C106), C100 (= C109), C104 (= C113), I109 (≠ P118), C139 (= C130), D140 (= D131), L141 (≠ G132), C142 (= C133), P148 (= P143), A149 (≠ S144), C150 (= C145), C154 (= C149), P155 (= P150), A158 (= A153), L159 (= L154)
P11349 Respiratory nitrate reductase 1 beta chain; Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta; EC 1.7.5.1 from Escherichia coli (strain K12) (see 2 papers)
34% identity, 49% coverage: 59:159/205 of query aligns to 173:273/512 of P11349
- C184 (= C70) binding
- C187 (= C73) binding
- C192 (= C78) binding
- C196 (= C82) binding
- C217 (= C103) binding
- C223 (= C109) binding
- C227 (= C113) binding
- C244 (= C130) binding
- C247 (= C133) binding
- C259 (= C145) binding
- C263 (= C149) binding
Sites not aligning to the query:
- 16 binding
- 19 binding
- 22 binding
- 26 binding
3egwB The crystal structure of the narghi mutant narh - c16a
34% identity, 49% coverage: 59:159/205 of query aligns to 173:273/509 of 3egwB
- binding fe3-s4 cluster: P181 (≠ S67), C196 (= C82), S198 (≠ T84), I201 (≠ M87), C217 (= C103), R218 (≠ I104), G219 (= G105), W220 (≠ C106), R221 (≠ E107), M222 (≠ S108), C223 (= C109), S241 (≠ M127), C263 (= C149), V264 (≠ P150), G265 (≠ Q151), I267 (≠ A153), R268 (≠ L154)
- binding protoporphyrin ix containing fe: W220 (≠ C106), R221 (≠ E107)
- binding iron/sulfur cluster: L183 (≠ S69), C184 (= C70), E185 (≠ N71), H186 (= H72), C187 (= C73), P190 (= P76), C192 (= C78), V210 (= V96), C227 (= C113), Y229 (= Y115), I232 (≠ P118), K243 (= K129), C244 (= C130), I245 (≠ D131), F246 (≠ G132), C247 (= C133), T257 (≠ P143), V258 (≠ S144), C259 (= C145)
Sites not aligning to the query:
7b04A of Nitrite oxidoreductase (Nxr) from the anammox bacterium Kuenenia stuttgartiensis.
26% identity, 69% coverage: 62:202/205 of query aligns to 166:305/409 of 7b04A
- binding fe3-s4 cluster: C186 (= C82), I191 (≠ M87), C207 (= C103), R208 (≠ I104), G209 (= G105), Y210 (≠ C106), R211 (≠ E107), K212 (≠ S108), C213 (= C109), S231 (≠ M127)
- binding protoporphyrin ix containing fe: P187 (= P83), R188 (≠ T84), R208 (≠ I104), Y210 (≠ C106)
- binding iron/sulfur cluster: Q171 (≠ S67), I173 (≠ S69), C174 (= C70), N175 (= N71), H176 (= H72), C177 (= C73), P180 (= P76), G181 (≠ V77), C182 (= C78), V200 (= V96), C217 (= C113), Y219 (= Y115), P222 (= P118), K233 (= K129), C234 (= C130), I235 (≠ D131), A236 (≠ G132), C237 (= C133), T255 (≠ P143), R256 (≠ S144), C257 (= C145), C261 (= C149), V262 (≠ I159), G263 (≠ A160), I265 (≠ L162), R266 (≠ K163)
Sites not aligning to the query:
- binding iron/sulfur cluster: 34, 35, 36, 37, 38, 39, 40, 44, 48, 59, 60, 62
6f0kB Alternative complex iii (see paper)
26% identity, 74% coverage: 6:157/205 of query aligns to 723:914/961 of 6f0kB
- binding fe3-s4 cluster: C803 (= C82), V805 (≠ T84), M818 (≠ V98), C823 (= C103), I824 (= I104), G825 (= G105), T826 (≠ C106), R827 (≠ E107), Y828 (≠ S108), C829 (= C109), M869 (= M127)
- binding heme c: A806 (≠ G85), N821 (≠ D101), R822 (≠ L102), R880 (≠ A138), Q883 (vs. gap), R884 (vs. gap), N887 (vs. gap)
- binding iron/sulfur cluster: C733 (= C16), T734 (≠ S17), G735 (= G18), C736 (= C19), N737 (≠ K20), A738 (≠ T21), C739 (= C22), C743 (= C26), W765 (= W38), L766 (≠ R39), I768 (≠ T41), C791 (= C70), M792 (≠ N71), H793 (= H72), C794 (= C73), P798 (≠ V77), C799 (= C78), N816 (≠ V96), C833 (= C113), R838 (vs. gap), C872 (= C130), Y874 (≠ G132), C875 (= C133), A901 (≠ S144), C902 (= C145), C906 (= C149)
Sites not aligning to the query:
6x6uB Wor5 from pyrococcus furiosus, taurine-bound (see paper)
29% identity, 72% coverage: 9:155/205 of query aligns to 5:131/167 of 6x6uB
- binding phosphate ion: D10 (≠ T14)
- binding iron/sulfur cluster: C12 (= C16), S13 (= S17), G14 (= G18), C15 (= C19), R16 (≠ K20), C18 (= C22), C22 (= C26), H26 (≠ Y44), R36 (≠ Q54), I37 (≠ G55), V39 (≠ Y57), P49 (≠ S67), C52 (= C70), V53 (≠ N71), Q54 (≠ H72), C55 (= C73), P59 (≠ V77), C60 (= C78), C64 (= C82), P65 (= P83), L69 (≠ M87), C85 (= C103), I86 (= I104), T87 (≠ G105), C88 (= C106), G89 (≠ E107), A90 (≠ S108), C91 (= C109), C95 (= C113), V99 (≠ A117), P100 (= P118), C112 (= C130), D113 (= D131), L114 (≠ G132), C115 (= C133), P119 (= P143), K120 (≠ S144), C121 (= C145), C125 (= C149), H130 (≠ L154)
Sites not aligning to the query:
8c0zC Cryoem structure of a tungsten-containing aldehyde oxidoreductase from aromatoleum aromaticum (see paper)
25% identity, 72% coverage: 9:156/205 of query aligns to 5:133/158 of 8c0zC