SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 206109 FitnessBrowser__DvH:206109 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P26276 Phosphomannomutase/phosphoglucomutase; PMM / PGM; EC 5.4.2.2; EC 5.4.2.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 10 papers)
46% identity, 98% coverage: 3:446/453 of query aligns to 8:453/463 of P26276

query
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P26276

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R15 (= R10) mutation to A: KM halves, decreases processivity as dissociation of G1,6P intermediate increases 25-fold.
Y17 (= Y12) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate
R20 (= R15) mutation to A: No phosphoglucomutase activity.
S108 (= S103) binding via phosphate group; modified: Phosphoserine; mutation S->A,V: About 5% activity, still subject to substrate inhibition and requires G1,6P as an activator; phosphorylation occurs at a different site.; mutation to C: KM for G1P unchanged, kcat decreases 24-fold; G1,6P stimulates reaction by 2-3 orders of magnitude. No stable protein phosphorylation detected, altered ligation of metal residue.
N110 (= N105) mutation to A: KM halves, decreases processivity as dissociation of G1,6P intermediate increases 30-fold.
D242 (= D237) binding Mg(2+)
D244 (= D239) binding Mg(2+)
D246 (= D241) binding Mg(2+)
R247 (= R242) mutation to A: Small reduction in KM, small increase in dissociation of G1,6P intermediate.
R262 (≠ E257) mutation to A: Increases KM 2-fold, decreases kcat 9-fold for G1P. Alters flexibility of the hinge region.
K285 (= K280) binding alpha-D-glucose 1-phosphate
H308 (= H303) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate
E325 (= E320) mutation to A: Reduces KM and Vmax approximately 2-fold.
EMSGH 325:329 (≠ ELSGH 320:324) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate
H329 (= H324) mutation to A: No phosphoglucomutase activity using G1P as substrate, protein is less easily phosphorylated, no significant change in structure.
P368 (= P362) mutation to G: Increases KM 2-fold, decreases kcat 6-fold for G1P. Alters flexibility of the hinge region, structure is less compact.
R421 (= R414) mutation to C: Loss of phosphomannomutase activity, very low phosphoglucomutase activity.
RASNT 421:425 (= RASNT 414:418) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Q02E40 Phosphomannomutase/phosphoglucomutase; PMM / PGM; EC 5.4.2.2; EC 5.4.2.8 from Pseudomonas aeruginosa (strain UCBPP-PA14) (see paper)
46% identity, 98% coverage: 3:446/453 of query aligns to 8:453/463 of Q02E40

query
sites
Q02E40

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S108 (= S103) active site, Non-phosphorylated intermediate; modified: Phosphoserine

2h5aX Complex of the enzyme pmm/pgm with xylose 1-phosphate (see paper)
46% identity, 98% coverage: 4:446/453 of query aligns to 1:445/455 of 2h5aX

query
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2h5aX

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E

V

T

G

G

A

A

P

L

L

L

A

A

G

G

E
|
E

L

M

S
|
S

G

G

H
|
H

M

V

F

F

G

K

D

E

R

R

W

W

F

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

G

S

A

A

R

R

L

L

V

L

E

E

L

I

L

L

A

S

A

Q

S

D

D

Q

V

R

P

D

L

S

T

E

D

H

L

V

-

F

P

S

G

A

W

F

P

P

P

S

S

D

H

I

A

S

T

T

R

P

E

E

L

I

H

N

L

I

P

T

C

V

P

T

E

E

H

D

A

S

K

K

F

F

E

A

V

I

R

E

R

A

A

L

Q

Q

-

R

-

D

A

A

Y

Q

F

W

R

G

E

E

R

-

C

G

T

N

I

I

N

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

I

D

F

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

Q

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

Q

D

T

T

P

E

E

E

R

E

L

L

A

E

E

R

I

I

R

K

A

T

F

V

V

F

E

R

E

N

P

Q

L

L

R

K

active site: H101 (= H104), D234 (= D237), D236 (= D239), D238 (= D241), R239 (= R242), D332 (= D335)
binding 1-O-phosphono-alpha-D-xylopyranose: Y9 (= Y12), T298 (= T301), G299 (= G302), H300 (= H303), E317 (= E320), S319 (= S322), H321 (= H324), R413 (= R414), S415 (= S416), N416 (= N417), T417 (= T418)
binding zinc ion: S100 (= S103), D234 (= D237), D236 (= D239), D238 (= D241)

2h4lX Complex of pmm/pgm with ribose 1-phosphate (see paper)
46% identity, 98% coverage: 4:446/453 of query aligns to 1:445/455 of 2h4lX

query
sites
2h4lX

L

L

S

P

A

A

H

S

V

I

F

F

R

R

A

A

Y
|
Y

D

D

I

I

R
|
R

G

G

I

V

V

V

D

G

T

D

D

T

F

L

D

T

P

A

E

E

W

T

V

A

E

Y

R

W

L

I

G

G

R

R

A

A

C

I

G

G

T

S

Y

E

F

S

V

L

S

A

H

R

G

G

H

E

G

P

A

C

A

V

V

A

V

V

G

G

F

R

D

D

C

G

R

R

H

L

S

S

S

G

P

P

A

E

Y

L

H

V

D

K

A

Q

L

L

V

I

R

Q

G

G

L

L

L

V

S

D

T

C

G

G

V

C

D

Q

V

V

T

S

S

D

V

V

G

G

M

M

V

V

P

P

T

T

P

P

V

V

L

L

Y

Y

F

Y

A

A

V

A

K

N

H

V

L

L

G

E

R

G

K

K

A

S

G

G

V

V

M

M

I

L

T

T

A

G

S
|
S

H
|
H

N

N

P

P

S

P

E

D

Y

Y

N

N

G

G

F

F

K

K

V

I

V

V

A

V

G

A

E

G

S

E

T

T

I

L

H

A

G

N

E

E

E

Q

I

I

R

Q

R

A

I

L

W

R

E

E

V

R

F

I

E

E

R

K

G

N

E

D

F

L

A

A

S

S

G

G

H

V

G

G

I

S

G

V

C

E

S

Q

H

V

D

D

I

I

V

L

P

P

S

R

Y

Y

I

F

E

K

A

Q

I

I

T

R

S

D

D

D

V

I

H

A

P

M

A

A

R

K

K

P

L

M

K

K

V

V

D

D

G

C

G

G

N

N

G

G

A

V

G

A

G

G

E

V

L

I

C

A

V

P

E

Q

V

L

L

I

R

E

R

A

L

L

G

G

V

C

E

S

V

V

V

I

A

P

Q

L

F

Y

C

C

E

E

P

V

D

D

G

G

D

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

V

G

V

K

E

P

A

E

N

N

M

L

T

K

A

D

L

L

M

I

E

A

R

K

V

V

Q

K

V

A

E

E

R

N

A

A

D

D

L

L

G

G

I

L

G

A

L

F

D
|
D

G

G

D
|
D

A

G

D
|
D

R
|
R

L

V

G

G

A

V

V

V

D

T

G

N

M

T

G

G

R

T

L

I

N

Y

G

P

D

D

E

R

L

L

S

M

L

L

Y

F

A

A

R

K

E

D

M

V

L

V

A

S

R

R

R

N

P

P

G

G

E

A

T

D

V

I

I

I

A

F

D

D

V

V

K

K

C

C

S

T

H

R

R

R

L

L

F

I

D

A

D

L

I

I

E

S

A

G

H

Y

G

G

K

R

P

P

M

V

M

M

W

W

I

K

T
|
T

G

G

H

H

S

S

V

L

V

I

K

K

A

K

R

K

M

M

L

K

E

E

V

T

G

G

A

A

P

L

L

L

A

A

G

G

E
|
E

L

M

S

S

G

G

H

H

M

V

F

F

G

K

D

E

R

R

W

W

F

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

G

S

A

A

R

R

L

L

V

L

E

E

L

I

L

L

A

S

A

Q

S

D

D

Q

V

R

P

D

L

S

T

E

D

H

L

V

-

F

P

S

G

A

W

F

P

P

P

S

S

D

H

I

A

S

T

T

R

P

E

E

L

I

H

N

L

I

P

T

C

V

P

T

E

E

H

D

A

S

K

K

F

F

E

A

V

I

R

E

R

A

A

L

Q

Q

-

R

-

D

A

A

Y

Q

F

W

R

G

E

E

R

-

C

G

T

N

I

I

N

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

I

D

F

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

Q

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

Q

D

T

T

P

E

E

E

R

E

L

L

A

E

E

R

I

I

R

K

A

T

F

V

V

F

E

R

E

N

P

Q

L

L

R

K

active site: H101 (= H104), D234 (= D237), D236 (= D239), D238 (= D241), R239 (= R242), D332 (= D335)
binding 1-O-phosphono-alpha-D-ribofuranose: Y9 (= Y12), R12 (= R15), S100 (= S103), T298 (= T301), E317 (= E320), R413 (= R414), S415 (= S416), N416 (= N417), T417 (= T418)
binding zinc ion: S100 (= S103), D234 (= D237), D236 (= D239), D238 (= D241)

2fkfA Phosphomannomutase/phosphoglucomutase from pseudomonas aeruginosa with alpha-d-glucose 1,6-bisphosphate bound (see paper)
46% identity, 98% coverage: 4:446/453 of query aligns to 1:445/455 of 2fkfA

query
sites
2fkfA

L

L

S

P

A

A

H

S

V

I

F

F

R
|
R

A

A

Y

Y

D

D

I

I

R
|
R

G

G

I

V

V

V

D

G

T

D

D

T

F

L

D

T

P

A

E

E

W

T

V

A

E

Y

R

W

L

I

G

G

R

R

A

A

C

I

G

G

T

S

Y

E

F

S

V

L

S

A

H

R

G

G

H

E

G

P

A

C

A

V

V

A

V

V

G

G

F

R

D

D

C

G

R

R

H

L

S

S

S

G

P

P

A

E

Y

L

H

V

D

K

A

Q

L

L

V

I

R

Q

G

G

L

L

L

V

S

D

T

C

G

G

V

C

D

Q

V

V

T

S

S

D

V

V

G

G

M

M

V

V

P

P

T

T

P

P

V

V

L

L

Y

Y

F

Y

A

A

V

A

K

N

H

V

L

L

G

E

R

G

K

K

A

S

G

G

V

V

M

M

I

L

T

T

A

G

S
|
S

H
|
H

N

N

P

P

S

P

E

D

Y

Y

N

N

G

G

F

F

K
|
K

V

I

V

V

A

V

G

A

E

G

S

E

T

T

I

L

H

A

G

N

E

E

E

Q

I

I

R

Q

R

A

I

L

W

R

E

E

V

R

F

I

E

E

R

K

G

N

E

D

F

L

A

A

S

S

G

G

H

V

G

G

I

S

G

V

C

E

S

Q

H

V

D

D

I

I

V

L

P

P

S

R

Y

Y

I

F

E

K

A

Q

I

I

T

R

S

D

D

D

V

I

H

A

P

M

A

A

R

K

K

P

L

M

K

K

V

V

D

D

G

C

G

G

N

N

G

G

A

V

G

A

G

G

E

V

L

I

C

A

V

P

E

Q

V

L

L

I

R

E

R

A

L

L

G

G

V

C

E

S

V

V

V

I

A

P

Q

L

F

Y

C

C

E

E

P

V

D

D

G

G

D

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

V

G

V

K

E

P

A

E

N

N

M

L

T

K

A

D

L

L

M

I

E

A

R

K

V

V

Q

K

V

A

E

E

R

N

A

A

D

D

L

L

G

G

I

L

G

A

L

F

D
|
D

G

G

D
|
D

A

G

D
|
D

R
|
R

L

V

G

G

A

V

V

V

D

T

G

N

M

T

G

G

R

T

L

I

N

Y

G

P

D

D

E

R

L

L

S

M

L

L

Y

F

A

A

R

K

E

D

M

V

L

V

A

S

R

R

R

N

P

P

G

G

E

A

T

D

V

I

I

I

A

F

D

D

V

V

K

K

C

C

S

T

H

R

R

R

L

L

F

I

D

A

D

L

I

I

E

S

A

G

H

Y

G

G

K

R

P

P

M

V

M

M

W

W

I

K

T

T

G

G

H

H

S

S

V

L

V

I

K

K

A

K

R

K

M

M

L

K

E

E

V

T

G

G

A

A

P

L

L

L

A

A

G

G

E

E

L

M

S
|
S

G

G

H
|
H

M

V

F

F

G

K

D

E

R

R

W

W

F

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

G

S

A

A

R

R

L

L

V

L

E

E

L

I

L

L

A

S

A

Q

S

D

D

Q

V

R

P

D

L

S

T

E

D

H

L

V

-

F

P

S

G

A

W

F

P

P

P

S

S

D

H

I

A

S

T

T

R

P

E

E

L

I

H

N

L

I

P

T

C

V

P

T

E

E

H

D

A

S

K

K

F

F

E

A

V

I

R

E

R

A

A

L

Q

Q

-

R

-

D

A

A

Y

Q

F

W

R

G

E

E

R

-

C

G

T

N

I

I

N

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

I

D

F

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

Q

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

Q

D

T

T

P

E

E

E

R

E

L

L

A

E

E

R

I

I

R

K

A

T

F

V

V

F

E

R

E

N

P

Q

L

L

R

K

active site: R12 (= R15), S100 (= S103), H101 (= H104), K110 (= K113), D234 (= D237), D236 (= D239), D238 (= D241), R239 (= R242), H321 (= H324), D332 (= D335)
binding 1,6-di-O-phosphono-alpha-D-glucopyranose: R7 (= R10), H101 (= H104), S319 (= S322), R413 (= R414), S415 (= S416), N416 (= N417), T417 (= T418)
binding zinc ion: S100 (= S103), D234 (= D237), D236 (= D239), D238 (= D241)

1pcmX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
46% identity, 98% coverage: 4:446/453 of query aligns to 1:445/455 of 1pcmX

query
sites
1pcmX

L

L

S

P

A

A

H

S

V

I

F

F

R

R

A

A

Y
|
Y

D

D

I

I

R
|
R

G

G

I

V

V

V

D

G

T

D

D

T

F

L

D

T

P

A

E

E

W

T

V

A

E

Y

R

W

L

I

G

G

R

R

A

A

C

I

G

G

T

S

Y

E

F

S

V

L

S

A

H

R

G

G

H

E

G

P

A

C

A

V

V

A

V

V

G

G

F

R

D

D

C

G

R

R

H

L

S

S

S

G

P

P

A

E

Y

L

H

V

D

K

A

Q

L

L

V

I

R

Q

G

G

L

L

L

V

S

D

T

C

G

G

V

C

D

Q

V

V

T

S

S

D

V

V

G

G

M

M

V

V

P

P

T

T

P

P

V

V

L

L

Y

Y

F

Y

A

A

V

A

K

N

H

V

L

L

G

E

R

G

K

K

A

S

G

G

V

V

M

M

I

L

T

T

A

G

S
|
S

H
|
H

N

N

P

P

S

P

E

D

Y

Y

N

N

G

G

F

F

K
|
K

V

I

V

V

A

V

G

A

E

G

S

E

T

T

I

L

H

A

G

N

E

E

E

Q

I

I

R

Q

R

A

I

L

W

R

E

E

V

R

F

I

E

E

R

K

G

N

E

D

F

L

A

A

S

S

G

G

H

V

G

G

I

S

G

V

C

E

S

Q

H

V

D

D

I

I

V

L

P

P

S

R

Y

Y

I

F

E

K

A

Q

I

I

T

R

S

D

D

D

V

I

H

A

P

M

A

A

R

K

K

P

L

M

K

K

V

V

D

D

G

C

G

G

N

N

G

G

A

V

G

A

G

G

E

V

L

I

C

A

V

P

E

Q

V

L

L

I

R

E

R

A

L

L

G

G

V

C

E

S

V

V

V

I

A

P

Q

L

F

Y

C

C

E

E

P

V

D

D

G

G

D

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

V

G

V

K

E

P

A

E

N

N

M

L

T

K

A

D

L

L

M

I

E

A

R

K

V

V

Q

K

V

A

E

E

R

N

A

A

D

D

L

L

G

G

I

L

G

A

L

F

D
|
D

G

G

D
|
D

A

G

D
|
D

R
|
R

L

V

G

G

A

V

V

V

D

T

G

N

M

T

G

G

R

T

L

I

N

Y

G

P

D

D

E

R

L

L

S

M

L

L

Y

F

A

A

R

K

E

D

M

V

L

V

A

S

R

R

R

N

P

P

G

G

E

A

T

D

V

I

I

I

A

F

D

D

V

V

K

K

C

C

S

T

H

R

R

R

L

L

F

I

D

A

D

L

I

I

E

S

A

G

H

Y

G

G

K

R

P

P

M

V

M

M

W

W

I

K

T
|
T

G
|
G

H
|
H

S

S

V

L

V

I

K

K

A

K

R

K

M

M

L

K

E

E

V

T

G

G

A

A

P

L

L

L

A

A

G

G

E
|
E

L

M

S
|
S

G

G

H
|
H

M

V

F

F

G

K

D

E

R

R

W

W

F

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

G

S

A

A

R

R

L

L

V

L

E

E

L

I

L

L

A

S

A

Q

S

D

D

Q

V

R

P

D

L

S

T

E

D

H

L

V

-

F

P

S

G

A

W

F

P

P

P

S

S

D

H

I

A

S

T

T

R

P

E

E

L

I

H

N

L

I

P

T

C

V

P

T

E

E

H

D

A

S

K

K

F

F

E

A

V

I

R

E

R

A

A

L

Q

Q

-

R

-

D

A

A

Y

Q

F

W

R

G

E

E

R

-

C

G

T

N

I

I

N

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

I

D

F

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R
|
R

A

A

S
|
S

N

N

T

T

Q

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

Q

D

T

T

P

E

E

E

R

E

L

L

A

E

E

R

I

I

R

K

A

T

F

V

V

F

E

R

E

N

P

Q

L

L

R

K

active site: R12 (= R15), S100 (= S103), H101 (= H104), K110 (= K113), D234 (= D237), D236 (= D239), D238 (= D241), R239 (= R242), H321 (= H324), D332 (= D335)
binding 6-O-phosphono-alpha-D-mannopyranose: Y9 (= Y12), S100 (= S103), T298 (= T301), G299 (= G302), H300 (= H303), E317 (= E320), S319 (= S322), H321 (= H324), R413 (= R414), S415 (= S416)
binding zinc ion: S100 (= S103), D234 (= D237), D236 (= D239), D238 (= D241)

1p5gX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
46% identity, 98% coverage: 4:446/453 of query aligns to 1:445/455 of 1p5gX

query
sites
1p5gX

L

L

S

P

A

A

H

S

V

I

F

F

R

R

A

A

Y
|
Y

D

D

I

I

R
|
R

G

G

I

V

V

V

D

G

T

D

D

T

F

L

D

T

P

A

E

E

W

T

V

A

E

Y

R

W

L

I

G

G

R

R

A

A

C

I

G

G

T

S

Y

E

F

S

V

L

S

A

H

R

G

G

H

E

G

P

A

C

A

V

V

A

V

V

G

G

F

R

D

D

C

G

R

R

H

L

S

S

S

G

P

P

A

E

Y

L

H

V

D

K

A

Q

L

L

V

I

R

Q

G

G

L

L

L

V

S

D

T

C

G

G

V

C

D

Q

V

V

T

S

S

D

V

V

G

G

M

M

V

V

P

P

T

T

P

P

V

V

L

L

Y

Y

F

Y

A

A

V

A

K

N

H

V

L

L

G

E

R

G

K

K

A

S

G

G

V

V

M

M

I

L

T

T

A

G

S
|
S

H
|
H

N

N

P

P

S

P

E

D

Y

Y

N

N

G

G

F

F

K
|
K

V

I

V

V

A

V

G

A

E

G

S

E

T

T

I

L

H

A

G

N

E

E

E

Q

I

I

R

Q

R

A

I

L

W

R

E

E

V

R

F

I

E

E

R

K

G

N

E

D

F

L

A

A

S

S

G

G

H

V

G

G

I

S

G

V

C

E

S

Q

H

V

D

D

I

I

V

L

P

P

S

R

Y

Y

I

F

E

K

A

Q

I

I

T

R

S

D

D

D

V

I

H

A

P

M

A

A

R

K

K

P

L

M

K

K

V

V

D

D

G

C

G

G

N

N

G

G

A

V

G

A

G

G

E

V

L

I

C

A

V

P

E

Q

V

L

L

I

R

E

R

A

L

L

G

G

V

C

E

S

V

V

V

I

A

P

Q

L

F

Y

C

C

E

E

P

V

D

D

G

G

D

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

V

G

V

K

E

P

A

E

N

N

M

L

T

K

A

D

L

L

M

I

E

A

R

K

V

V

Q

K

V

A

E

E

R

N

A

A

D

D

L

L

G

G

I

L

G

A

L

F

D
|
D

G

G

D
|
D

A

G

D
|
D

R
|
R

L

V

G

G

A

V

V

V

D

T

G

N

M

T

G

G

R

T

L

I

N

Y

G

P

D

D

E

R

L

L

S

M

L

L

Y

F

A

A

R

K

E

D

M

V

L

V

A

S

R

R

R

N

P

P

G

G

E

A

T

D

V

I

I

I

A

F

D

D

V

V

K
|
K

C

C

S

T

H

R

R

R

L

L

F

I

D

A

D

L

I

I

E

S

A

G

H

Y

G

G

K

R

P

P

M

V

M

M

W

W

I

K

T

T

G
|
G

H
|
H

S

S

V

L

V

I

K

K

A

K

R

K

M

M

L

K

E

E

V

T

G

G

A

A

P

L

L

L

A

A

G

G

E
|
E

L

M

S
|
S

G

G

H
|
H

M

V

F

F

G

K

D

E

R

R

W

W

F

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

G

S

A

A

R

R

L

L

V

L

E

E

L

I

L

L

A

S

A

Q

S

D

D

Q

V

R

P

D

L

S

T

E

D

H

L

V

-

F

P

S

G

A

W

F

P

P

P

S

S

D

H

I

A

S

T

T

R

P

E

E

L

I

H

N

L

I

P

T

C

V

P

T

E

E

H

D

A

S

K

K

F

F

E

A

V

I

R

E

R

A

A

L

Q

Q

-

R

-

D

A

A

Y

Q

F

W

R

G

E

E

R

-

C

G

T

N

I

I

N

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

I

D

F

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

Q

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

Q

D

T

T

P

E

E

E

R

E

L

L

A

E

E

R

I

I

R

K

A

T

F

V

V

F

E

R

E

N

P

Q

L

L

R

K

active site: R12 (= R15), S100 (= S103), H101 (= H104), K110 (= K113), D234 (= D237), D236 (= D239), D238 (= D241), R239 (= R242), H321 (= H324), D332 (= D335)
binding 6-O-phosphono-alpha-D-glucopyranose: Y9 (= Y12), S100 (= S103), K277 (= K280), G299 (= G302), H300 (= H303), E317 (= E320), S319 (= S322), H321 (= H324), R413 (= R414), S415 (= S416), N416 (= N417), T417 (= T418)
binding zinc ion: S100 (= S103), D234 (= D237), D236 (= D239), D238 (= D241)

1p5dX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
46% identity, 98% coverage: 4:446/453 of query aligns to 1:445/455 of 1p5dX

query
sites
1p5dX

L

L

S

P

A

A

H

S

V

I

F

F

R

R

A

A

Y
|
Y

D

D

I

I

R
|
R

G

G

I

V

V

V

D

G

T

D

D

T

F

L

D

T

P

A

E

E

W

T

V

A

E

Y

R

W

L

I

G

G

R

R

A

A

C

I

G

G

T

S

Y

E

F

S

V

L

S

A

H

R

G

G

H

E

G

P

A

C

A

V

V

A

V

V

G

G

F

R

D

D

C

G

R

R

H

L

S

S

S

G

P

P

A

E

Y

L

H

V

D

K

A

Q

L

L

V

I

R

Q

G

G

L

L

L

V

S

D

T

C

G

G

V

C

D

Q

V

V

T

S

S

D

V

V

G

G

M

M

V

V

P

P

T

T

P

P

V

V

L

L

Y

Y

F

Y

A

A

V

A

K

N

H

V

L

L

G

E

R

G

K

K

A

S

G

G

V

V

M

M

I

L

T

T

A

G

S
|
S

H
|
H

N

N

P

P

S

P

E

D

Y

Y

N

N

G

G

F

F

K
|
K

V

I

V

V

A

V

G

A

E

G

S

E

T

T

I

L

H

A

G

N

E

E

E

Q

I

I

R

Q

R

A

I

L

W

R

E

E

V

R

F

I

E

E

R

K

G

N

E

D

F

L

A

A

S

S

G

G

H

V

G

G

I

S

G

V

C

E

S

Q

H

V

D

D

I

I

V

L

P

P

S

R

Y

Y

I

F

E

K

A

Q

I

I

T

R

S

D

D

D

V

I

H

A

P

M

A

A

R

K

K

P

L

M

K

K

V

V

D

D

G

C

G

G

N

N

G

G

A

V

G

A

G

G

E

V

L

I

C

A

V

P

E

Q

V

L

L

I

R

E

R

A

L

L

G

G

V

C

E

S

V

V

V

I

A

P

Q

L

F

Y

C

C

E

E

P

V

D

D

G

G

D

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

V

G

V

K

E

P

A

E

N

N

M

L

T

K

A

D

L

L

M

I

E

A

R

K

V

V

Q

K

V

A

E

E

R

N

A

A

D

D

L

L

G

G

I

L

G

A

L

F

D
|
D

G

G

D
|
D

A

G

D
|
D

R
|
R

L

V

G

G

A

V

V

V

D

T

G

N

M

T

G

G

R

T

L

I

N

Y

G

P

D

D

E

R

L

L

S

M

L

L

Y

F

A

A

R

K

E

D

M

V

L

V

A

S

R

R

R

N

P

P

G

G

E

A

T

D

V

I

I

I

A

F

D

D

V

V

K

K

C

C

S

T

H

R

R

R

L

L

F

I

D

A

D

L

I

I

E

S

A

G

H

Y

G

G

K

R

P

P

M

V

M

M

W

W

I

K

T
|
T

G
|
G

H
|
H

S

S

V

L

V

I

K

K

A

K

R

K

M

M

L

K

E

E

V

T

G

G

A

A

P

L

L

L

A

A

G

G

E
|
E

L

M

S
|
S

G

G

H
|
H

M

V

F

F

G

K

D

E

R

R

W

W

F

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

G

S

A

A

R

R

L

L

V

L

E

E

L

I

L

L

A

S

A

Q

S

D

D

Q

V

R

P

D

L

S

T

E

D

H

L

V

-

F

P

S

G

A

W

F

P

P

P

S

S

D

H

I

A

S

T

T

R

P

E

E

L

I

H

N

L

I

P

T

C

V

P

T

E

E

H

D

A

S

K

K

F

F

E

A

V

I

R

E

R

A

A

L

Q

Q

-

R

-

D

A

A

Y

Q

F

W

R

G

E

E

R

-

C

G

T

N

I

I

N

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

I

D

F

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R
|
R

A

A

S
|
S

N

N

T
|
T

Q

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

Q

D

T

T

P

E

E

E

R

E

L

L

A

E

E

R

I

I

R

K

A

T

F

V

V

F

E

R

E

N

P

Q

L

L

R

K

active site: R12 (= R15), S100 (= S103), H101 (= H104), K110 (= K113), D234 (= D237), D236 (= D239), D238 (= D241), R239 (= R242), H321 (= H324), D332 (= D335)
binding 1-O-phosphono-alpha-D-glucopyranose: Y9 (= Y12), S100 (= S103), R239 (= R242), T298 (= T301), G299 (= G302), H300 (= H303), E317 (= E320), S319 (= S322), H321 (= H324), R413 (= R414), S415 (= S416), T417 (= T418)
binding zinc ion: S100 (= S103), D234 (= D237), D236 (= D239), D238 (= D241)

1pcjX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
46% identity, 98% coverage: 3:446/453 of query aligns to 3:448/458 of 1pcjX

query
sites
1pcjX

A

T

L

L

S

P

A

A

H

S

V

I

F

F

R

R

A

A

Y
|
Y

D

D

I

I

R
|
R

G

G

I

V

V

V

D

G

T

D

D

T

F

L

D

T

P

A

E

E

W

T

V

A

E

Y

R

W

L

I

G

G

R

R

A

A

C

I

G

G

T

S

Y

E

F

S

V

L

S

A

H

R

G

G

H

E

G

P

A

C

A

V

V

A

V

V

G

G

F

R

D

D

C

G

R

R

H

L

S

S

S

G

P

P

A

E

Y

L

H

V

D

K

A

Q

L

L

V

I

R

Q

G

G

L

L

L

V

S

D

T

C

G

G

V

C

D

Q

V

V

T

S

S

D

V

V

G

G

M

M

V

V

P

P

T

T

P

P

V

V

L

L

Y

Y

F

Y

A

A

V

A

K

N

H

V

L

L

G

E

R

G

K

K

A

S

G

G

V

V

M

M

I

L

T

T

A

G

S
|
S

H
|
H

N

N

P

P

S

P

E

D

Y

Y

N

N

G

G

F

F

K
|
K

V

I

V

V

A

V

G

A

E

G

S

E

T

T

I

L

H

A

G

N

E

E

E

Q

I

I

R

Q

R

A

I

L

W

R

E

E

V

R

F

I

E

E

R

K

G

N

E

D

F

L

A

A

S

S

G

G

H

V

G

G

I

S

G

V

C

E

S

Q

H

V

D

D

I

I

V

L

P

P

S

R

Y

Y

I

F

E

K

A

Q

I

I

T

R

S

D

D

D

V

I

H

A

P

M

A

A

R

K

K

P

L

M

K

K

V

V

D

D

G

C

G

G

N

N

G

G

A

V

G

A

G

G

E

V

L

I

C

A

V

P

E

Q

V

L

L

I

R

E

R

A

L

L

G

G

V

C

E

S

V

V

V

I

A

P

Q

L

F

Y

C

C

E

E

P

V

D

D

G

G

D

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

V

G

V

K

E

P

A

E

N

N

M

L

T

K

A

D

L

L

M

I

E

A

R

K

V

V

Q

K

V

A

E

E

R

N

A

A

D

D

L

L

G

G

I

L

G

A

L

F

D
|
D

G

G

D
|
D

A

G

D
|
D

R
|
R

L

V

G

G

A

V

V

V

D

T

G

N

M

T

G

G

R

T

L

I

N

Y

G

P

D

D

E

R

L

L

S

M

L

L

Y

F

A

A

R

K

E

D

M

V

L

V

A

S

R

R

R

N

P

P

G

G

E

A

T

D

V

I

I

I

A

F

D

D

V

V

K

K

C

C

S

T

H

R

R

R

L

L

F

I

D

A

D

L

I

I

E

S

A

G

H

Y

G

G

K

R

P

P

M

V

M

M

W

W

I

K

T
|
T

G
|
G

H

H

S

S

V

L

V

I

K

K

A

K

R

K

M

M

L

K

E

E

V

T

G

G

A

A

P

L

L

L

A

A

G

G

E
|
E

L

M

S
|
S

G

G

H
|
H

M

V

F

F

G

K

D

E

R

R

W

W

F

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

G

S

A

A

R

R

L

L

V

L

E

E

L

I

L

L

A

S

A

Q

S

D

D

Q

V

R

P

D

L

S

T

E

D

H

L

V

-

F

P

S

G

A

W

F

P

P

P

S

S

D

H

I

A

S

T

T

R

P

E

E

L

I

H

N

L

I

P

T

C

V

P

T

E

E

H

D

A

S

K

K

F

F

E

A

V

I

R

E

R

A

A

L

Q

Q

-

R

-

D

A

A

Y

Q

F

W

R

G

E

E

R

-

C

G

T

N

I

I

N

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

I

D

F

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

Q

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

Q

D

T

T

P

E

E

E

R

E

L

L

A

E

E

R

I

I

R

K

A

T

F

V

V

F

E

R

E

N

P

Q

L

L

R

K

active site: R15 (= R15), S103 (= S103), H104 (= H104), K113 (= K113), D237 (= D237), D239 (= D239), D241 (= D241), R242 (= R242), H324 (= H324), D335 (= D335)
binding 1-O-phosphono-alpha-D-mannopyranose: Y12 (= Y12), S103 (= S103), T301 (= T301), G302 (= G302), E320 (= E320), S322 (= S322), H324 (= H324), R416 (= R414), S418 (= S416), N419 (= N417), T420 (= T418)
binding zinc ion: S103 (= S103), D237 (= D237), D239 (= D239), D241 (= D241)

1k2yX Crystal structure of phosphomannomutase/phosphoglucomutase s108a mutant from p. Aeruginosa (see paper)
45% identity, 98% coverage: 3:446/453 of query aligns to 4:449/459 of 1k2yX

query
sites
1k2yX

A

T

L

L

S

P

A

A

H

S

V

I

F

F

R

R

A

A

Y

Y

D

D

I

I

R

R

G

G

I

V

V

V

D

G

T

D

D

T

F

L

D

T

P

A

E

E

W

T

V

A

E

Y

R

W

L

I

G

G

R

R

A

A

C

I

G

G

T

S

Y

E

F

S

V

L

S

A

H

R

G

G

H

E

G

P

A

C

A

V

V

A

V

V

G

G

F

R

D

D

C

G

R

R

H

L

S

S

S

G

P

P

A

E

Y

L

H

V

D

K

A

Q

L

L

V

I

R

Q

G

G

L

L

L

V

S

D

T

C

G

G

V

C

D

Q

V

V

T

S

S

D

V

V

G

G

M

M

V

V

P

P

T

T

P

P

V

V

L

L

Y

Y

F

Y

A

A

V

A

K

N

H

V

L

L

G

E

R

G

K

K

A

S

G

G

V

V

M

M

I

L

T

T

A

G

S

A

H
|
H

N

N

P

P

S

P

E

D

Y

Y

N

N

G

G

F

F

K

K

V

I

V

V

A

V

G

A

E

G

S

E

T

T

I

L

H

A

G

N

E

E

E

Q

I

I

R

Q

R

A

I

L

W

R

E

E

V

R

F

I

E

E

R

K

G

N

E

D

F

L

A

A

S

S

G

G

H

V

G

G

I

S

G

V

C

E

S

Q

H

V

D

D

I

I

V

L

P

P

S

R

Y

Y

I

F

E

K

A

Q

I

I

T

R

S

D

D

D

V

I

H

A

P

M

A

A

R

K

K

P

L

M

K

K

V

V

D

D

G

C

G

G

N

N

G

G

A

V

G

A

G

G

E

V

L

I

C

A

V

P

E

Q

V

L

L

I

R

E

R

A

L

L

G

G

V

C

E

S

V

V

V

I

A

P

Q

L

F

Y

C

C

E

E

P

V

D

D

G

G

D

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

V

G

V

K

E

P

A

E

N

N

M

L

T

K

A

D

L

L

M

I

E

A

R

K

V

V

Q

K

V

A

E

E

R

N

A

A

D

D

L

L

G

G

I

L

G

A

L

F

D
|
D

G

G

D
|
D

A

G

D
|
D

R
|
R

L

V

G

G

A

V

V

V

D

T

G

N

M

T

G

G

R

T

L

I

N

Y

G

P

D

D

E

R

L

L

S

M

L

L

Y

F

A

A

R

K

E

D

M

V

L

V

A

S

R

R

R

N

P

P

G

G

E

A

T

D

V

I

I

I

A

F

D

D

V

V

K

K

C

C

S

T

H

R

R

R

L

L

F

I

D

A

D

L

I

I

E

S

A

G

H

Y

G

G

K

R

P

P

M

V

M

M

W

W

I

K

T

T

G

G

H

H

S

S

V

L

V

I

K

K

A

K

R

K

M

M

L

K

E

E

V

T

G

G

A

A

P

L

L

L

A

A

G

G

E

E

L

M

S

S

G

G

H

H

M

V

F

F

G

K

D

E

R

R

W

W

F

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

G

S

A

A

R

R

L

L

V

L

E

E

L

I

L

L

A

S

A

Q

S

D

D

Q

V

R

P

D

L

S

T

E

D

H

L

V

-

F

P

S

G

A

W

F

P

P

P

S

S

D

H

I

A

S

T

T

R

P

E

E

L

I

H

N

L

I

P

T

C

V

P

T

E

E

H

D

A

S

K

K

F

F

E

A

V

I

R

E

R

A

A

L

Q

Q

-

R

-

D

A

A

Y

Q

F

W

R

G

E

E

R

-

C

G

T

N

I

I

N

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

I

D

F

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R

R

A

A

S

S

N

N

T

T

Q

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

Q

D

T

T

P

E

E

E

R

E

L

L

A

E

E

R

I

I

R

K

A

T

F

V

V

F

E

R

E

N

P

Q

L

L

R

K

active site: H105 (= H104), D238 (= D237), D240 (= D239), D242 (= D241), R243 (= R242), D336 (= D335)
binding zinc ion: D238 (= D237), D240 (= D239), D242 (= D241)

4il8A Crystal structure of an h329a mutant of p. Aeruginosa pmm/pgm (see paper)
45% identity, 98% coverage: 3:446/453 of query aligns to 4:449/459 of 4il8A

query
sites
4il8A

A

T

L

L

S

P

A

A

H

S

V

I

F

F

R

R

A

A

Y

Y

D

D

I

I

R
|
R

G

G

I

V

V

V

D

G

T

D

D

T

F

L

D

T

P

A

E

E

W

T

V

A

E

Y

R

W

L

I

G

G

R

R

A

A

C

I

G

G

T

S

Y

E

F

S

V

L

S

A

H

R

G

G

H

E

G

P

A

C

A

V

V

A

V

V

G

G

F

R

D

D

C

G

R

R

H

L

S

S

S

G

P

P

A

E

Y

L

H

V

D

K

A

Q

L

L

V

I

R

Q

G

G

L

L

L

V

S

D

T

C

G

G

V

C

D

Q

V

V

T

S

S

D

V

V

G

G

M

M

V

V

P

P

T

T

P

P

V

V

L

L

Y

Y

F

Y

A

A

V

A

K

N

H

V

L

L

G

E

R

G

K

K

A

S

G

G

V

V

M

M

I

L

T

T

A

G

S
|
S

H
|
H

N

N

P

P

S

P

E

D

Y

Y

N

N

G

G

F

F

K
|
K

V

I

V

V

A

V

G

A

E

G

S

E

T

T

I

L

H

A

G

N

E

E

E

Q

I

I

R

Q

R

A

I

L

W

R

E

E

V

R

F

I

E

E

R

K

G

N

E

D

F

L

A

A

S

S

G

G

H

V

G

G

I

S

G

V

C

E

S

Q

H

V

D

D

I

I

V

L

P

P

S

R

Y

Y

I

F

E

K

A

Q

I

I

T

R

S

D

D

D

V

I

H

A

P

M

A

A

R

K

K

P

L

M

K

K

V

V

D

D

G

C

G

G

N

N

G

G

A

V

G

A

G

G

E

V

L

I

C

A

V

P

E

Q

V

L

L

I

R

E

R

A

L

L

G

G

V

C

E

S

V

V

V

I

A

P

Q

L

F

Y

C

C

E

E

P

V

D

D

G

G

D

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

V

G

V

K

E

P

A

E

N

N

M

L

T

K

A

D

L

L

M

I

E

A

R

K

V

V

Q

K

V

A

E

E

R

N

A

A

D

D

L

L

G

G

I

L

G

A

L

F

D
|
D

G

G

D
|
D

A

G

D
|
D

R
|
R

L

V

G

G

A

V

V

V

D

T

G

N

M

T

G

G

R

T

L

I

N

Y

G

P

D

D

E

R

L

L

S

M

L

L

Y

F

A

A

R

K

E

D

M

V

L

V

A

S

R

R

R

N

P

P

G

G

E

A

T

D

V

I

I

I

A

F

D

D

V

V

K

K

C

C

S

T

H

R

R

R

L

L

F

I

D

A

D

L

I

I

E

S

A

G

H

Y

G

G

K

R

P

P

M

V

M

M

W

W

I

K

T

T

G

G

H

H

S

S

V

L

V

I

K

K

A

K

R

K

M

M

L

K

E

E

V

T

G

G

A

A

P

L

L

L

A

A

G

G

E

E

L

M

S

S

G

G

H
x
A

M

V

F

F

G

K

D

E

R

R

W

W

F

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

G

S

A

A

R

R

L

L

V

L

E

E

L

I

L

L

A

S

A

Q

S

D

D

Q

V

R

P

D

L

S

T

E

D

H

L

V

-

F

P

S

G

A

W

F

P

P

P

S

S

D

H

I

A

S

T

T

R

P

E

E

L

I

H

N

L

I

P

T

C

V

P

T

E

E

H

D

A

S

K

K

F

F

E

A

V

I

R

E

R

A

A

L

Q

Q

-

R

-

D

A

A

Y

Q

F

W

R

G

E

E

R

-

C

G

T

N

I

I

N

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

I

D

F

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R

R

A

A

S

S

N

N

T

T

Q

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

Q

D

T

T

P

E

E

E

R

E

L

L

A

E

E

R

I

I

R

K

A

T

F

V

V

F

E

R

E

N

P

Q

L

L

R

K

active site: R16 (= R15), S104 (= S103), H105 (= H104), K114 (= K113), D238 (= D237), D240 (= D239), D242 (= D241), R243 (= R242), A325 (≠ H324), D336 (= D335)
binding magnesium ion: S104 (= S103), D238 (= D237), D240 (= D239), D242 (= D241)

3rsmA Crystal structure of s108c mutant of pmm/pgm (see paper)
45% identity, 92% coverage: 30:446/453 of query aligns to 14:426/436 of 3rsmA

query
sites
3rsmA

L

I

G

G

R

R

A

A

C

I

G

G

T

S

Y

E

F

S

V

L

S

A

H

R

G

G

H

E

G

P

A

C

A

V

V

A

V

V

G

G

F

R

D

D

C

G

R

R

H

L

S

S

S

G

P

P

A

E

Y

L

H

V

D

K

A

Q

L

L

V

I

R

Q

G

G

L

L

L

V

S

D

T

C

G

G

V

C

D

Q

V

V

T

S

S

D

V

V

G

G

M

M

V

V

P

P

T

T

P

P

V

V

L

L

Y

Y

F

Y

A

A

V

A

K

N

H

V

L

L

G

E

R

G

K

K

A

S

G

G

V

V

M

M

I

L

T

T

A

G

S
x
C

H

-

N

-

P

-

S

-

E

-

Y

-

N

N

G

G

F

F

K
|
K

V

I

V

V

A

V

G

A

E

G

S

E

T

T

I

L

H

A

G

N

E

E

E

Q

I

I

R

Q

R

A

I

L

W

R

E

E

V

R

F

I

E

E

R

K

G

N

E

D

F

L

A

A

S

S

G

G

H

V

G

G

I

S

G

V

C

E

S

Q

H

V

D

D

I

I

V

L

P

P

S

R

Y

Y

I

F

E

K

A

Q

I

I

T

R

S

D

D

D

V

I

H

A

P

M

A

A

R

K

K

P

L

M

K

K

V

V

D

D

G

C

G

G

N

N

G

G

A

V

G

A

G

G

E

V

L

I

C

A

V

P

E

Q

V

L

L

I

R

E

R

A

L

L

G

G

V

C

E

S

V

V

V

I

A

P

Q

L

F

Y

C

C

E

E

P

V

D

D

G

G

D

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

V

G

V

K

E

P

A

E

N

N

M

L

T

K

A

D

L

L

M

I

E

A

R

K

V

V

Q

K

V

A

E

E

R

N

A

A

D

D

L

L

G

G

I

L

G

A

L

F

D
|
D

G

G

D
|
D

A

G

D
|
D

R
|
R

L

V

G

G

A

V

V

V

D

T

G

N

M

T

G

G

R

T

L

I

N

Y

G

P

D

D

E

R

L

L

S

M

L

L

Y

F

A

A

R

K

E

D

M

V

L

V

A

S

R

R

R

N

P

P

G

G

E

A

T

D

V

I

I

I

A

F

D

D

V

V

K

K

C

C

S

T

H

R

R

R

L

L

F

I

D

A

D

L

I

I

E

S

A

G

H

Y

G

G

K

R

P

P

M

V

M

M

W

W

I

K

T

T

G

G

H

H

S

S

V

L

V

I

K

K

A

K

R

K

M

M

L

K

E

E

V

T

G

G

A

A

P

L

L

L

A

A

G

G

E

E

L

M

S

S

G

G

H
|
H

M

V

F

F

G

K

D

E

R

R

W

W

F

F

G

G

F

F

D
|
D

D

D

A

G

I

I

Y

Y

G

S

A

A

R

R

L

L

V

L

E

E

L

I

L

L

A

S

A

Q

S

D

D

Q

V

R

P

D

L

S

T

E

D

H

L

V

-

F

P

S

G

A

W

F

P

P

P

S

S

D

H

I

A

S

T

T

R

P

E

E

L

I

H

N

L

I

P

T

C

V

P

T

E

E

H

D

A

S

K

K

F

F

E

A

V

I

R

E

R

A

A

L

Q

Q

-

R

-

D

A

A

Y

Q

F

W

R

G

E

E

R

-

C

G

T

N

I

I

N

T

D

T

I

L

D

D

G

G

A

V

R

R

V

V

I

D

F

Y

P

P

D

K

G

G

W

W

G

G

L

L

V

V

R

R

A

A

S

S

N

N

T

T

Q

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

Q

D

T

T

P

E

E

E

R

E

L

L

A

E

E

R

I

I

R

K

A

T

F

V

V

F

E

R

E

N

P

Q

L

L

R

K

active site: C87 (≠ S103), K91 (= K113), D215 (= D237), D217 (= D239), D219 (= D241), R220 (= R242), H302 (= H324), D313 (= D335)
binding phosphate ion: C87 (≠ S103), D215 (= D237), D217 (= D239), D219 (= D241), R220 (= R242)
binding zinc ion: D215 (= D237), D217 (= D239), D219 (= D241)

3uw2A X-ray crystal structure of phosphoglucomutase/phosphomannomutase family protein (bth_i1489)from burkholderia thailandensis (see paper)
44% identity, 96% coverage: 4:438/453 of query aligns to 2:440/458 of 3uw2A

query
sites
3uw2A

L

I

S

S

A

Q

H

S

V

I

F

F

R

K

A

A

Y

Y

D

D

I

I

R
|
R

G

G

I

V

V

I

D

G

T

K

D

T

F

L

D

D

P

A

E

D

W

V

V

A

E

R

R

S

L

I

G

G

R

R

A

A

C

F

G

G

T

S

Y

E

F

V

V

R

S

A

H

Q

G

G

H

G

G

D

A

A

A

V

V

V

G

A

F

R

D

D

C

G

R

R

H

L

S

S

S

G

P

P

A

E

Y

L

H

V

D

G

A

A

L

L

V

A

R

D

G

G

L

L

L

R

S

A

T

A

G

G

V

V

D

D

V

V

T

V

S

D

V

V

G

G

M

M

V

V

P

P

T

T

P

P

V

V

L

G

Y

Y

F

F

A

A

V

A

K

S

-

V

H

P

L

L

G

A

R

L

K

S

A

G

G

G

-

E

-

R

-

V

-

D

-

S

-

C

V

I

M

V

I

V

T

T

A

G

S
|
S

H
|
H

N

N

P

P

S

P

E

D

Y

Y

N

N

G

G

F

F

K
|
K

V

M

V

V

A

L

G

R

E

G

S

A

T

A

I

I

H

Y

G

G

E

D

E

Q

I

I

R

Q

R

G

I

L

W

Y

E

K

V

R

F

I

E

V

R

D

G

A

E

R

F

F

A

E

S

T

G

G

H

S

G

G

I

S

G

Y

C

E

S

Q

H

Y

D

D

I

V

V

A

P

D

S

Q

Y

Y

I

V

E

E

A

R

I

I

T

V

S

G

D

D

V

I

H

K

P

L

A

T

R

R

K

P

L

L

K

K

V

L

V

V

D

D

G

A

G

G

N

N

G

G

A

V

G

A

G

G

E

P

L

L

C

A

V

T

E

R

V

L

L

F

R

K

R

A

L

L

G

G

V

C

E

E

V

L

V

V

A

E

Q

L

F

F

C

T

E

D

P

I

D

D

G

G

D

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

V

A

V

H

E

P

A

E

N

N

M

L

T

Q

A

D

L

V

M

I

E

A

R

K

V

L

Q

K

V

A

E

T

R

D

A

A

D

E

L

I

G

G

I

F

G

A

L

F

D
|
D

G

G

D
|
D

A

G

D
|
D

R
|
R

L

L

G

G

A

V

V

V

D

T

G

K

M

D

G

G

R

Q

L

I

N

Y

G

P

D

D

E

R

L

Q

L

L

S

M

L

L

Y

F

A

A

R

E

E

E

M

V

L

L

A

S

R

R

R

N

P

P

G

G

E

A

T

Q

V

I

I

I

A

Y

D

D

V

V

K

K

C

C

S

T

H

R

R

N

L

L

F

A

D

R

D

W

I

V

E

R

A

E

H

K

G

G

K

E

P

P

M

L

M

M

W

W

I

K

T

T

G

G

H

H

S

S

V

L

V

V

K

K

A

A

R

K

M

L

L

R

E

E

V

T

G

G

A

A

P

P

L

L

A

A

G

G

E

E

L

M

S

S

G

G

H
|
H

M

V

F

F

G

K

D

D

R

R

W

W

F

Y

G

G

F

F

D

D

A

G

I

L

Y

Y

G

T

A

G

A

A

R

R

L

L

V

L

E

E

L

I

L

L

A

A

A

R

S

V

D

A

V

D

P

P

L

S

T

A

D

L

L

L

P

N

G

G

W

L

P

P

P

N

S

A

H

V

A

S

T

T

R

P

E

E

L

L

H

Q

L

L

P

-

C

-

P

-

E

-

H

N

A

V

K

K

F

L

E

I

V

D

V

K

R

L

R

R

A

A

Q

D

A

A

Y

K

F

F

R

D

E

G

R

A

C

D

T

E

I

V

N

V

D

T

I

I

D

D

G

G

A

L

R

R

V

V

I

E

F

Y

P

P

D

D

G

G

W

F

G

G

L

L

V

A

R

R

A

S

S

S

N

N

T

T

Q

T

P

P

V

V

L

V

V

V

L

L

R

R

F

F

E

E

A

A

Q

T

T

S

P

D

E

A

R

A

L

L

A

A

E

R

I

I

R

Q

active site: R13 (= R15), S109 (= S103), H110 (= H104), K119 (= K113), D243 (= D237), D245 (= D239), D247 (= D241), R248 (= R242), H330 (= H324)
binding zinc ion: D243 (= D237), D245 (= D239), D247 (= D241)

1wqaA Crystal structure of pyrococcus horikoshii phosphomannomutase/phosphoglucomutase complexed with mg2+
36% identity, 95% coverage: 7:436/453 of query aligns to 3:441/455 of 1wqaA

query
sites
1wqaA

H

K

V

L

F

F

R

G

A

T

Y

F

D

G

I

V

R
|
R

G

G

I

I

V

A

D

N

T

E

D

K

F

I

D

T

P

P

E

E

W

F

V

A

E

M

R

K

L

I

G

G

R

M

A

A

C

F

G

G

T

T

Y

L

F

L

V

K

S

R

H

E

G

G

H

R

G

K

A

K

-

P

-

L

A

V

V

V

G

G

F

R

D

D

C

T

R

R

H

V

S

S

S

G

P

E

A

M

Y

L

H

K

D

E

A

A

L

L

V

I

R

S

G

G

L

L

S

S

T

V

G

G

V

C

D

D

V

V

T

I

S

D

V

V

G

G

M

I

V

A

P

P

T

T

P

P

V

A

L

V

Y

Q

F

W

A

A

V

T

K

K

H

H

L

F

G

N

R

A

K

D

A

G

G

G

V

A

M

V

I

I

T

T

A

A

S
|
S

H
|
H

N

N

P

P

S

P

E

E

Y

Y

N

N

G

G

F

I

K
|
K

V

L

A

E

G

P

E

N

S

G

T

M

-

G

I

L

H

K

G

K

E

E

E

R

I

E

R

A

R

I

I

V

W

E

E

E

V

L

F

F

E

F

R

K

G

E

E

D

F

F

-

D

-

R

A

A

S

K

G

W

H

Y

G

E

I

I

G

G

-

E

-

V

C

R

S

R

H

E

D

D

I

I

V

I

P

K

S

P

Y

Y

I

I

E

E

A

A

I

I

T

K

S

S

-

K

-

V

D

D

V

V

H

E

P

A

A

I

R

K

K

K

L

R

K

K

-

P

-

F

V

V

D

D

G

T

G

S

N

N

G

G

A

A

G

G

G

S

E

L

L

T

C

L

V

P

E

Y

V

L

L

L

R

R

R

E

L

L

G

G

V

C

E

K

V

V

V

I

A

T

Q

V

F

N

C

A

E

Q

P

P

D

D

G

G

D

Y

F

F

P

P

N

A

H

R

H

N

P

P

D

E

P

P

V

-

V

N

E

E

A

E

N

N

M

L

T

K

A

E

L

F

M

M

E

E

R

I

V

V

Q

K

V

A

E

L

R

G

A

A

D

D

L

F

G

G

I

V

G

A

L

Q

D
|
D

G

G

D
|
D

A

A

D
|
D

R
|
R

L

A

G

V

A

F

V

I

D

D

G

E

M

N

G

G

R

R

L

F

L

I

N

Q

G

G

D

D

E

K

L

T

L

F

S

A

L

L

Y

V

A

A

R

D

E

A

M

V

L

L

A

K

R

E

R

K

P

G

G

G

E

G

T

L

V

L

I

V

A

T

D

T

V

V

K

A

C

T

S

S

H

N

R

L

L

L

F

D

D

D

D

I

I

A

E

K

A

K

H

H

G

G

G

A

K

K

P

V

M

M

M

R

W

T

I

K

T

V

G

G

H

D

S

L

V

I

V

V

K

A

A

R

R

A

M

L

L

Y

E

E

V

N

G

N

A

G

P

T

L

I

A

G

G

G

E

E

L

E

S

N

G

G

H
x
G

M

V

F

I

F

F

G

P

D

E

R

H

W

V

F

L

G

G

F
x
R

D

D

D

G

A

A

I

M

Y

T

G

-

A

V

A

A

R

K

L

V

V

V

E

E

L

I

L

F

A

A

A

K

S

S

D

G

V

K

P

K

L

F

T

S

D

E

L

L

P

I

G

D

W

E

P

L

P

P

S

K

H

Y

-

Y

-

Q

A

I

T

K

R

T

E

K

L

R

H

H

L

V

P

E

C

G

P

D

E

R

H

H

A

A

K

-

F

-

E

-

V

I

V

V

R

N

R

K

A

V

Q

A

A

E

Y

M

F

A

R

R

E

E

R

R

-

G

C

Y

T

T

I

V

N

D

D

T

I

T

D

D

G

G

A

A

R

K

V

I

I

I

F

F

P

E

D

D

G

G

W

W

G

V

L

L

V

V

R

R

A

A

S

S

N

G

T

T

Q

E

P

P

V

I

L

I

V

R

L

I

R

F

F

S

E

E

A

A

Q

K

T

S

P

K

E

E

R

K

L

A

A

Q

E

E

active site: R11 (= R15), S101 (= S103), H102 (= H104), K111 (= K113), D243 (= D237), D245 (= D239), D247 (= D241), R248 (= R242), G330 (≠ H324), R340 (≠ F334)
binding magnesium ion: S101 (= S103), D243 (= D237), D245 (= D239), D247 (= D241)

6nqhA Xanthomonas citri dephospho-pgm in complex with xylose-1-phosphate
32% identity, 95% coverage: 9:438/453 of query aligns to 6:438/448 of 6nqhA

query
sites
6nqhA

F

F

R

K

A

A

Y

Y

D

D

I

I

R
|
R

G

G

I

R

V

V

D

P

T

D

D

E

F

L

D

N

P

E

E

D

W

L

V

A

E

R

R

R

L

I

G

G

R

V

A

A

C

L

G

A

T

A

Y

Q

F

L

V

-

S

-

H

-

G

D

H

Q

G

G

A

P

A

V

V

V

V

L

G

G

F

H

D

D

C

V

R

R

H

L

S

A

S

S

P

P

A

A

Y

L

H

Q

D

E

A

A

L

L

V

S

R

A

G

G

L

L

L

R

S

A

T

S

G

G

V

R

D

D

V

V

T

I

S

D

V

I

G

G

M

L

V

C

P

G

T

T

P

E

V

E

L

V

Y

Y

F

F

A

Q

V

T

K

D

H

Y

L

L

G

K

R

A

K

A

A

G

G

G

V

V

M

M

I

V

T

T

A

A

S
|
S

H
|
H

N

N

P

P

S

M

E

D

Y

Y

N

N

G

G

F

M

K
|
K

V

L

V

V

A

R

G

E

E

Q

S

A

T

R

I

P

H

I

G

S

E

S

E

D

-

T

-

G

I

L

R

F

R

A

I

I

W

R

E

D

V

T

F

V

E

A

R

A

G

D

E

T

F

A

A

A

S

P

G

G

H

E

G

P

I

T

G

A

C

S

-

E

-

Q

S

S

H

R

D

T

I

D

V

K

P

T

S

A

Y

Y

I

L

E

E

A

H

I

L

T

L

S

S

-

Y

-

V

D

D

V

R

H

S

P

T

A

L

R

K

K

P

L

L

K

K

V

L

V

V

D

N

G

A

G

G

N

N

G

G

A

G

G

A

G

G

E

L

L

I

C

V

V

D

E

L

V

L

L

A

R

P

R

H

L

L

G

P

V

F

E

E

V

F

V

V

A

R

Q

V

F

F

C

H

E

E

P

P

D

D

G

G

D

N

F

F

P

P

N

N

H

G

H

I

P

P

D

N

P

P

V

L

E

P

A

E

N

N

M

R

T

D

A

A

L

T

M

A

E

K

R

A

V

V

Q

K

V

D

E

N

R

G

A

A

D

D

L

F

G

G

I

I

G

A

L

W

D
|
D

G

G

D
|
D

A

F

D
|
D

R
|
R

L

C

G

F

A

F

V

F

D

D

G

H

M

T

G

G

R

R

L

F

L

I

N

E

G

G

D

Y

E

Y

L

L

L

V

S

G

L

L

Y

L

A

A

R

Q

E

A

M

I

L

L

A

A

R

K

R

Q

P

P

G

G

E

G

T

K

V

V

I

V

A

H

D

D

V

P

K
x
R

C

L

S

T

H

W

R

N

L

T

F

V

D

E

D

Q

I

V

E

E

A

E

H

A

G

G

K

I

P

P

M

V

M

L

W

C

I

K

T
x
S

G
|
G

H

H

S

A

V

F

V

I

K

K

A

E

R

K

M

M

L

R

E

S

V

E

G

N

A

A

P

V

L

Y

A

G

G

G

E
|
E

L

M

S
|
S

G

A

H
|
H

M

H

F

Y

F

F

G

-

D

-

R

R

W

E

F

F

G

A

F

Y

-

A

D

D

D

S

A

G

I

M

Y

I

G

P

A

W

A

L

R

L

L

I

V

A

E

E

L

L

L

V

A

S

A

Q

S

S

D

G

V

R

P

S

L

L

T

A

D

D

L

L

P

V

G

-

W

-

P

-

S

-

H

-

A

E

T

A

R

R

E

M

L

Q

H

K

L

F

P

P

C

C

P

S

-

G

-

E

-

I

-

N

-

F

-

K

-

V

E

A

H

D

A

A

K

K

F

A

E

S

V

V

V

A

R

R

R

V

A

M

Q

E

A

H

Y

Y

F

A

R

S

E

L

R

S

C

P

T

E

I

L

N

D

D

Y

I

T

D

D

G

G

A

I

R

S

V

A

I

D

F

F

P

G

D

Q

G

W

W

R

G

F

L

N

V

L

R
|
R

A

S

S
|
S

N
|
N

T
|
T

Q

E

P

P

V

L

L

L

V
x
R

L

L

R

N

F

V

E

E

A

T

Q

R

T

G

P

D

E

A

R

A

L

L

A

L

E

E

I

T

R

R

active site: R12 (= R15), S97 (= S103), H98 (= H104), K107 (= K113), D237 (= D237), D239 (= D239), D241 (= D241), R242 (= R242), H324 (= H324)
binding magnesium ion: D237 (= D237), D239 (= D239), D241 (= D241)
binding 1-O-phosphono-alpha-D-xylopyranose: R12 (= R15), S97 (= S103), H98 (= H104), K107 (= K113), D239 (= D239), R242 (= R242), R280 (≠ K280), S301 (≠ T301), G302 (= G302), E320 (= E320), S322 (= S322), H324 (= H324), R414 (= R414), S416 (= S416), N417 (= N417), T418 (= T418), R423 (≠ V423)

6np8A Xanthomonas citri phospho-pgm in complex with mannose-6-phosphate (see paper)
32% identity, 95% coverage: 9:438/453 of query aligns to 6:438/448 of 6np8A

query
sites
6np8A

F

F

R

K

A

A

Y
|
Y

D

D

I

I

R
|
R

G

G

I

R

V

V

D

P

T

D

D

E

F

L

D

N

P

E

E

D

W

L

V

A

E

R

R

R

L

I

G

G

R

V

A

A

C

L

G

A

T

A

Y

Q

F

L

V

-

S

-

H

-

G

D

H

Q

G

G

A

P

A

V

V

V

V

L

G

G

F

H

D

D

C

V

R

R

H

L

S

A

S

S

P

P

A

A

Y

L

H

Q

D

E

A

A

L

L

V

S

R

A

G

G

L

L

L

R

S

A

T

S

G

G

V

R

D

D

V

V

T

I

S

D

V

I

G

G

M

L

V

C

P

G

T

T

P

E

V

E

L

V

Y

Y

F

F

A

Q

V

T

K

D

H

Y

L

L

G

K

R

A

K

A

A

G

G

G

V

V

M

M

I

V

T

T

A

A

S
|
S

H
|
H

N

N

P

P

S

M

E

D

Y

Y

N

N

G

G

F

M

K
|
K

V

L

V

V

A

R

G

E

E

Q

S

A

T

R

I

P

H

I

G

S

E

S

E

D

-

T

-

G

I

L

R

F

R

A

I

I

W

R

E

D

V

T

F

V

E

A

R

A

G

D

E

T

F

A

A

A

S

P

G

G

H

E

G

P

I

T

G

A

C

S

-

E

-

Q

S

S

H

R

D

T

I

D

V

K

P

T

S

A

Y

Y

I

L

E

E

A

H

I

L

T

L

S

S

-

Y

-

V

D

D

V

R

H

S

P

T

A

L

R

K

K

P

L

L

K

K

V

L

V

V

D

N

G

A

G

G

N

N

G

G

A

G

G

A

G

G

E

L

L

I

C

V

V

D

E

L

V

L

L

A

R

P

R

H

L

L

G

P

V

F

E

E

V

F

V

V

A

R

Q

V

F

F

C

H

E

E

P

P

D

D

G

G

D

N

F

F

P

P

N

N

H

G

H

I

P

P

D

N

P

P

V

L

E

P

A

E

N

N

M

R

T

D

A

A

L

T

M

A

E

K

R

A

V

V

Q

K

V

D

E

N

R

G

A

A

D

D

L

F

G

G

I

I

G

A

L

W

D
|
D

G

G

D
|
D

A

F

D
|
D

R
|
R

L

C

G

F

A

F

V

F

D

D

G

H

M

T

G

G

R

R

L

F

L

I

N

E

G

G

D

Y

E

Y

L

L

L

V

S

G

L

L

Y

L

A

A

R

Q

E

A

M

I

L

L

A

A

R

K

R

Q

P

P

G

G

E

G

T

K

V

V

I

V

A

H

D

D

V

P

K
x
R

C

L

S

T

H

W

R

N

L

T

F

V

D

E

D

Q

I

V

E

E

A

E

H

A

G

G

K

I

P

P

M

V

M

L

W

C

I

K

T

S

G
|
G

H
|
H

S

A

V

F

V

I

K

K

A

E

R

K

M

M

L

R

E

S

V

E

G

N

A

A

P

V

L

Y

A

G

G

G

E
|
E

L

M

S
|
S

G

A

H
|
H

M

H

F

Y

F

F

G

-

D

-

R

R

W

E

F

F

G

A

F

Y

-

A

D

D

D

S

A

G

I

M

Y

I

G

P

A

W

A

L

R

L

L

I

V

A

E

E

L

L

L

V

A

S

A

Q

S

S

D

G

V

R

P

S

L

L

T

A

D

D

L

L

P

V

G

-

W

-

P

-

S

-

H

-

A

E

T

A

R

R

E

M

L

Q

H

K

L

F

P

P

C

C

P

S

-

G

-

E

-

I

-

N

-

F

-

K

-

V

E

A

H

D

A

A

K

K

F

A

E

S

V

V

V

A

R

R

R

V

A

M

Q

E

A

H

Y

Y

F

A

R

S

E

L

R

S

C

P

T

E

I

L

N

D

D

Y

I

T

D

D

G

G

A

I

R

S

V

A

I

D

F

F

P

G

D

Q

G

W

W

R

G

F

L

N

V

L

R
|
R

A

S

S
|
S

N
|
N

T
|
T

Q

E

P

P

V

L

L

L

V
x
R

L

L

R

N

F

V

E

E

A

T

Q

R

T

G

P

D

E

A

R

A

L

L

A

L

E

E

I

T

R

R

active site: R12 (= R15), S97 (= S103), H98 (= H104), K107 (= K113), D237 (= D237), D239 (= D239), D241 (= D241), R242 (= R242), H324 (= H324)
binding calcium ion: S97 (= S103), D237 (= D237), D239 (= D239), D241 (= D241)
binding 6-O-phosphono-alpha-D-mannopyranose: Y9 (= Y12), R280 (≠ K280), G302 (= G302), H303 (= H303), E320 (= E320), S322 (= S322), H324 (= H324), R414 (= R414), S416 (= S416), N417 (= N417), T418 (= T418), R423 (≠ V423)

6nolA Xanthomonas citri dephospho-pgm in complex with mannose-1-phosphate (see paper)
32% identity, 95% coverage: 9:438/453 of query aligns to 6:438/448 of 6nolA

query
sites
6nolA

F

F

R

K

A

A

Y

Y

D

D

I

I

R
|
R

G

G

I

R

V

V

D

P

T

D

D

E

F

L

D

N

P

E

E

D

W

L

V

A

E

R

R

R

L

I

G

G

R

V

A

A

C

L

G

A

T

A

Y

Q

F

L

V

-

S

-

H

-

G

D

H

Q

G

G

A

P

A

V

V

V

V

L

G

G

F

H

D

D

C

V

R

R

H

L

S

A

S

S

P

P

A

A

Y

L

H

Q

D

E

A

A

L

L

V

S

R

A

G

G

L

L

L

R

S

A

T

S

G

G

V

R

D

D

V

V

T

I

S

D

V

I

G

G

M

L

V

C

P

G

T

T

P

E

V

E

L

V

Y

Y

F

F

A

Q

V

T

K

D

H

Y

L

L

G

K

R

A

K

A

A

G

G

G

V

V

M

M

I

V

T

T

A

A

S
|
S

H
|
H

N

N

P

P

S

M

E

D

Y

Y

N

N

G

G

F

M

K
|
K

V

L

V

V

A

R

G

E

E

Q

S

A

T

R

I

P

H

I

G

S

E

S

E

D

-

T

-

G

I

L

R

F

R

A

I

I

W

R

E

D

V

T

F

V

E

A

R

A

G

D

E

T

F

A

A

A

S

P

G

G

H

E

G

P

I

T

G

A

C

S

-

E

-

Q

S

S

H

R

D

T

I

D

V

K

P

T

S

A

Y

Y

I

L

E

E

A

H

I

L

T

L

S

S

-

Y

-

V

D

D

V

R

H

S

P

T

A

L

R

K

K

P

L

L

K

K

V

L

V

V

D

N

G

A

G

G

N

N

G

G

A

G

G

A

G

G

E

L

L

I

C

V

V

D

E

L

V

L

L

A

R

P

R

H

L

L

G

P

V

F

E

E

V

F

V

V

A

R

Q

V

F

F

C

H

E

E

P

P

D

D

G

G

D

N

F

F

P

P

N

N

H

G

H

I

P

P

D

N

P

P

V

L

E

P

A

E

N

N

M

R

T

D

A

A

L

T

M

A

E

K

R

A

V

V

Q

K

V

D

E

N

R

G

A

A

D

D

L

F

G

G

I

I

G

A

L

W

D
|
D

G

G

D
|
D

A

F

D
|
D

R
|
R

L

C

G

F

A

F

V

F

D

D

G

H

M

T

G

G

R

R

L

F

L

I

N

E

G

G

D

Y

E

Y

L

L

L

V

S

G

L

L

Y

L

A

A

R

Q

E

A

M

I

L

L

A

A

R

K

R

Q

P

P

G

G

E

G

T

K

V

V

I

V

A

H

D

D

V

P

K

R

C

L

S

T

H

W

R

N

L

T

F

V

D

E

D

Q

I

V

E

E

A

E

H

A

G

G

K

I

P

P

M

V

M

L

W

C

I

K

T

S

G
|
G

H

H

S

A

V

F

V

I

K

K

A

E

R

K

M

M

L

R

E

S

V

E

G

N

A

A

P

V

L

Y

A

G

G

G

E
|
E

L

M

S
|
S

G

A

H
|
H

M

H

F

Y

F

F

G

-

D

-

R

R

W

E

F

F

G

A

F

Y

-

A

D

D

D

S

A

G

I

M

Y

I

G

P

A

W

A

L

R

L

L

I

V

A

E

E

L

L

L

V

A

S

A

Q

S

S

D

G

V

R

P

S

L

L

T

A

D

D

L

L

P

V

G

-

W

-

P

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S

-

H

-

A

E

T

A

R

R

E

M

L

Q

H

K

L

F

P

P

C

C

P

S

-

G

-

E

-

I

-

N

-

F

-

K

-

V

E

A

H

D

A

A

K

K

F

A

E

S

V

V

V

A

R

R

R

V

A

M

Q

E

A

H

Y

Y

F

A

R

S

E

L

R

S

C

P

T

E

I

L

N

D

D

Y

I

T

D

D

G

G

A

I

R

S

V

A

I

D

F

F

P

G

D

Q

G

W

W

R

G

F

L

N

V

L

R
|
R

A

S

S
|
S

N
|
N

T
|
T

Q

E

P

P

V

L

L

L

V
x
R

L

L

R

N

F

V

E

E

A

T

Q

R

T

G

P

D

E

A

R

A

L

L

A

L

E

E

I

T

R

R

active site: R12 (= R15), S97 (= S103), H98 (= H104), K107 (= K113), D237 (= D237), D239 (= D239), D241 (= D241), R242 (= R242), H324 (= H324)
binding 1-O-phosphono-alpha-D-mannopyranose: G302 (= G302), E320 (= E320), S322 (= S322), H324 (= H324), R414 (= R414), S416 (= S416), N417 (= N417), T418 (= T418), R423 (≠ V423)
binding magnesium ion: S97 (= S103), D237 (= D237), D239 (= D239), D241 (= D241)

6nnpA Xanthomonas citri dephospho-pgm in complex with glucose-6-phosphate (see paper)
32% identity, 95% coverage: 9:438/453 of query aligns to 6:438/448 of 6nnpA

query
sites
6nnpA

F

F

R

K

A

A

Y

Y

D

D

I

I

R
|
R

G

G

I

R

V

V

D

P

T

D

D

E

F

L

D

N

P

E

E

D

W

L

V

A

E

R

R

R

L

I

G

G

R

V

A

A

C

L

G

A

T

A

Y

Q

F

L

V

-

S

-

H

-

G

D

H

Q

G

G

A

P

A

V

V

V

V

L

G

G

F

H

D

D

C

V

R

R

H

L

S

A

S

S

P

P

A

A

Y

L

H

Q

D

E

A

A

L

L

V

S

R

A

G

G

L

L

L

R

S

A

T

S

G

G

V

R

D

D

V

V

T

I

S

D

V

I

G

G

M

L

V

C

P

G

T

T

P

E

V

E

L

V

Y

Y

F

F

A

Q

V

T

K

D

H

Y

L

L

G

K

R

A

K

A

A

G

G

G

V

V

M

M

I

V

T

T

A

A

S
|
S

H
|
H

N

N

P

P

S

M

E

D

Y

Y

N

N

G

G

F

M

K
|
K

V

L

V

V

A

R

G

E

E

Q

S

A

T

R

I

P

H

I

G

S

E

S

E

D

-

T

-

G

I

L

R

F

R

A

I

I

W

R

E

D

V

T

F

V

E

A

R

A

G

D

E

T

F

A

A

A

S

P

G

G

H

E

G

P

I

T

G

A

C

S

-

E

-

Q

S

S

H

R

D

T

I

D

V

K

P

T

S

A

Y

Y

I

L

E

E

A

H

I

L

T

L

S

S

-

Y

-

V

D

D

V

R

H

S

P

T

A

L

R

K

K

P

L

L

K

K

V

L

V

V

D

N

G

A

G

G

N

N

G

G

A

G

G

A

G

G

E

L

L

I

C

V

V

D

E

L

V

L

L

A

R

P

R

H

L

L

G

P

V

F

E

E

V

F

V

V

A

R

Q

V

F

F

C

H

E

E

P

P

D

D

G

G

D

N

F

F

P

P

N

N

H

G

H

I

P

P

D

N

P

P

V

L

E

P

A

E

N

N

M

R

T

D

A

A

L

T

M

A

E

K

R

A

V

V

Q

K

V

D

E

N

R

G

A

A

D

D

L

F

G

G

I

I

G

A

L

W

D
|
D

G

G

D
|
D

A

F

D
|
D

R
|
R

L

C

G

F

A

F

V

F

D

D

G

H

M

T

G

G

R

R

L

F

L

I

N

E

G

G

D

Y

E

Y

L

L

L

V

S

G

L

L

Y

L

A

A

R

Q

E

A

M

I

L

L

A

A

R

K

R

Q

P

P

G

G

E

G

T

K

V

V

I

V

A

H

D

D

V

P

K
x
R

C

L

S

T

H

W

R

N

L

T

F

V

D

E

D

Q

I

V

E

E

A

E

H

A

G

G

K

I

P

P

M

V

M

L

W

C

I

K

T

S

G
|
G

H
|
H

S

A

V

F

V

I

K

K

A

E

R

K

M

M

L

R

E

S

V

E

G

N

A

A

P

V

L

Y

A

G

G

G

E
|
E

L

M

S

S

G

A

H
|
H

M

H

F

Y

F

F

G

-

D

-

R

R

W

E

F

F

G

A

F

Y

-

A

D

D

D

S

A

G

I

M

Y

I

G

P

A

W

A

L

R

L

L

I

V

A

E

E

L

L

L

V

A

S

A

Q

S

S

D

G

V

R

P

S

L

L

T

A

D

D

L

L

P

V

G

-

W

-

P

-

S

-

H

-

A

E

T

A

R

R

E

M

L

Q

H

K

L

F

P

P

C

C

P

S

-

G

-

E

-

I

-

N

-

F

-

K

-

V

E

A

H

D

A

A

K

K

F

A

E

S

V

V

V

A

R

R

R

V

A

M

Q

E

A

H

Y

Y

F

A

R

S

E

L

R

S

C

P

T

E

I

L

N

D

D

Y

I

T

D

D

G

G

A

I

R

S

V

A

I

D

F

F

P

G

D

Q

G

W

W

R

G

F

L

N

V

L

R
|
R

A

S

S
|
S

N
|
N

T
|
T

Q

E

P

P

V

L

L

L

V
x
R

L

L

R

N

F

V

E

E

A

T

Q

R

T

G

P

D

E

A

R

A

L

L

A

L

E

E

I

T

R

R

active site: R12 (= R15), S97 (= S103), H98 (= H104), K107 (= K113), D237 (= D237), D239 (= D239), D241 (= D241), R242 (= R242), H324 (= H324)
binding 6-O-phosphono-alpha-D-glucopyranose: R280 (≠ K280), G302 (= G302), H303 (= H303), E320 (= E320), H324 (= H324), R414 (= R414), S416 (= S416), N417 (= N417), T418 (= T418), R423 (≠ V423)
binding magnesium ion: S97 (= S103), D237 (= D237), D239 (= D239), D241 (= D241)

6nn2A Xanthomonas citri pgm apo-phospho (see paper)
32% identity, 95% coverage: 9:438/453 of query aligns to 6:438/448 of 6nn2A

query
sites
6nn2A

F

F

R

K

A

A

Y

Y

D

D

I

I

R
|
R

G

G

I

R

V

V

D

P

T

D

D

E

F

L

D

N

P

E

E

D

W

L

V

A

E

R

R

R

L

I

G

G

R

V

A

A

C

L

G

A

T

A

Y

Q

F

L

V

-

S

-

H

-

G

D

H

Q

G

G

A

P

A

V

V

V

V

L

G

G

F

H

D

D

C

V

R

R

H

L

S

A

S

S

P

P

A

A

Y

L

H

Q

D

E

A

A

L

L

V

S

R

A

G

G

L

L

L

R

S

A

T

S

G

G

V

R

D

D

V

V

T

I

S

D

V

I

G

G

M

L

V

C

P

G

T

T

P

E

V

E

L

V

Y

Y

F

F

A

Q

V

T

K

D

H

Y

L

L

G

K

R

A

K

A

A

G

G

G

V

V

M

M

I

V

T

T

A

A

S
|
S

H
|
H

N

N

P

P

S

M

E

D

Y

Y

N

N

G

G

F

M

K
|
K

V

L

V

V

A

R

G

E

E

Q

S

A

T

R

I

P

H

I

G

S

E

S

E

D

-

T

-

G

I

L

R

F

R

A

I

I

W

R

E

D

V

T

F

V

E

A

R

A

G

D

E

T

F

A

A

A

S

P

G

G

H

E

G

P

I

T

G

A

C

S

-

E

-

Q

S

S

H

R

D

T

I

D

V

K

P

T

S

A

Y

Y

I

L

E

E

A

H

I

L

T

L

S

S

-

Y

-

V

D

D

V

R

H

S

P

T

A

L

R

K

K

P

L

L

K

K

V

L

V

V

D

N

G

A

G

G

N

N

G

G

A

G

G

A

G

G

E

L

L

I

C

V

V

D

E

L

V

L

L

A

R

P

R

H

L

L

G

P

V

F

E

E

V

F

V

V

A

R

Q

V

F

F

C

H

E

E

P

P

D

D

G

G

D

N

F

F

P

P

N

N

H

G

H

I

P

P

D

N

P

P

V

L

E

P

A

E

N

N

M

R

T

D

A

A

L

T

M

A

E

K

R

A

V

V

Q

K

V

D

E

N

R

G

A

A

D

D

L

F

G

G

I

I

G

A

L

W

D
|
D

G

G

D
|
D

A

F

D
|
D

R
|
R

L

C

G

F

A

F

V

F

D

D

G

H

M

T

G

G

R

R

L

F

L

I

N

E

G

G

D

Y

E

Y

L

L

L

V

S

G

L

L

Y

L

A

A

R

Q

E

A

M

I

L

L

A

A

R

K

R

Q

P

P

G

G

E

G

T

K

V

V

I

V

A

H

D

D

V

P

K

R

C

L

S

T

H

W

R

N

L

T

F

V

D

E

D

Q

I

V

E

E

A

E

H

A

G

G

K

I

P

P

M

V

M

L

W

C

I

K

T

S

G

G

H

H

S

A

V

F

V

I

K

K

A

E

R

K

M

M

L

R

E

S

V

E

G

N

A

A

P

V

L

Y

A

G

G

G

E

E

L

M

S

S

G

A

H
|
H

M

H

F

Y

F

F

G

-

D

-

R

R

W

E

F

F

G

A

F

Y

-

A

D

D

D

S

A

G

I

M

Y

I

G

P

A

W

A

L

R

L

L

I

V

A

E

E

L

L

L

V

A

S

A

Q

S

S

D

G

V

R

P

S

L

L

T

A

D

D

L

L

P

V

G

-

W

-

P

-

S

-

H

-

A

E

T

A

R

R

E

M

L

Q

H

K

L

F

P

P

C

C

P

S

-

G

-

E

-

I

-

N

-

F

-

K

-

V

E

A

H

D

A

A

K

K

F

A

E

S

V

V

V

A

R

R

R

V

A

M

Q

E

A

H

Y

Y

F

A

R

S

E

L

R

S

C

P

T

E

I

L

N

D

D

Y

I

T

D

D

G

G

A

I

R

S

V

A

I

D

F

F

P

G

D

Q

G

W

W

R

G

F

L

N

V

L

R

R

A

S

S

S

N

N

T

T

Q

E

P

P

V

L

L

L

V

R

L

L

R

N

F

V

E

E

A

T

Q

R

T

G

P

D

E

A

R

A

L

L

A

L

E

E

I

T

R

R

active site: R12 (= R15), S97 (= S103), H98 (= H104), K107 (= K113), D237 (= D237), D239 (= D239), D241 (= D241), R242 (= R242), H324 (= H324)
binding calcium ion: S97 (= S103), D237 (= D237), D239 (= D239), D241 (= D241)

6n1eA Crystal structure of x. Citri phosphoglucomutase in complex with 1- methyl-glucose 6-phosphate (see paper)
32% identity, 95% coverage: 9:438/453 of query aligns to 6:438/448 of 6n1eA

query
sites
6n1eA

F

F

R

K

A

A

Y

Y

D

D

I

I

R

R

G

G

I

R

V

V

D

P

T

D

D

E

F

L

D

N

P

E

E

D

W

L

V

A

E

R

R

R

L

I

G

G

R

V

A

A

C

L

G

A

T

A

Y

Q

F

L

V

-

S

-

H

-

G

D

H

Q

G

G

A

P

A

V

V

V

V

L

G

G

F

H

D

D

C

V

R

R

H

L

S

A

S

S

P

P

A

A

Y

L

H

Q

D

E

A

A

L

L

V

S

R

A

G

G

L

L

L

R

S

A

T

S

G

G

V

R

D

D

V

V

T

I

S

D

V

I

G

G

M

L

V

C

P

G

T

T

P

E

V

E

L

V

Y

Y

F

F

A

Q

V

T

K

D

H

Y

L

L

G

K

R

A

K

A

A

G

G

G

V

V

M

M

I

V

T

T

A

A

S
|
S

H

H

N

N

P

P

S

M

E

D

Y

Y

N

N

G

G

F

M

K

K

V

L

V

V

A

R

G

E

E

Q

S

A

T

R

I

P

H

I

G

S

E

S

E

D

-

T

-

G

I

L

R

F

R

A

I

I

W

R

E

D

V

T

F

V

E

A

R

A

G

D

E

T

F

A

A

A

S

P

G

G

H

E

G

P

I

T

G

A

C

S

-

E

-

Q

S

S

H

R

D

T

I

D

V

K

P

T

S

A

Y

Y

I

L

E

E

A

H

I

L

T

L

S

S

-

Y

-

V

D

D

V

R

H

S

P

T

A

L

R

K

K

P

L

L

K

K

V

L

V

V

D

N

G

A

G

G

N

N

G

G

A

G

G

A

G

G

E

L

L

I

C

V

V

D

E

L

V

L

L

A

R

P

R

H

L

L

G

P

V

F

E

E

V

F

V

V

A

R

Q

V

F

F

C

H

E

E

P

P

D

D

G

G

D

N

F

F

P

P

N

N

H

G

H

I

P

P

D

N

P

P

V

L

E

P

A

E

N

N

M

R

T

D

A

A

L

T

M

A

E

K

R

A

V

V

Q

K

V

D

E

N

R

G

A

A

D

D

L

F

G

G

I

I

G

A

L

W

D
|
D

G

G

D
|
D

A

F

D
|
D

R

R

L

C

G

F

A

F

V

F

D

D

G

H

M

T

G

G

R

R

L

F

L

I

N

E

G

G

D

Y

E

Y

L

L

L

V

S

G

L

L

Y

L

A

A

R

Q

E

A

M

I

L

L

A

A

R

K

R

Q

P

P

G

G

E

G

T

K

V

V

I

V

A

H

D

D

V

P

K

R

C

L

S

T

H

W

R

N

L

T

F

V

D

E

D

Q

I

V

E

E

A

E

H

A

G

G

K

I

P

P

M

V

M

L

W

C

I

K

T

S

G
|
G

H

H

S

A

V

F

V

I

K

K

A

E

R

K

M

M

L

R

E

S

V

E

G

N

A

A

P

V

L

Y

A

G

G

G

E
|
E

L

M

S

S

G

A

H
|
H

M

H

F

Y

F

F

G

-

D

-

R

R

W

E

F

F

G

A

F

Y

-

A

D

D

D

S

A

G

I

M

Y

I

G

P

A

W

A

L

R

L

L

I

V

A

E

E

L

L

L

V

A

S

A

Q

S

S

D

G

V

R

P

S

L

L

T

A

D

D

L

L

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G

-

W

-

P

-

S

-

H

-

A

E

T

A

R

R

E

M

L

Q

H

K

L

F

P

P

C

C

P

S

-

G

-

E

-

I

-

N

-

F

-

K

-

V

E

A

H

D

A

A

K

K

F

A

E

S

V

V

V

A

R

R

R

V

A

M

Q

E

A

H

Y

Y

F

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R

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R

S

C

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T

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L

N

D

D

Y

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T

D

D

G

G

A

I

R

S

V

A

I

D

F

F

P

G

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G

W

W

R

G

F

L

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V

L

R
|
R

A

S

S
|
S

N

N

T

T

Q

E

P

P

V

L

L

L

V
x
R

L

L

R

N

F

V

E

E

A

T

Q

R

T

G

P

D

E

A

R

A

L

L

A

L

E

E

I

T

R

R

binding magnesium ion: S97 (= S103), D237 (= D237), D239 (= D239), D241 (= D241)
binding 1-deoxy-7-O-phosphono-alpha-D-gluco-hept-2-ulopyranose: G302 (= G302), E320 (= E320), H324 (= H324), R414 (= R414), S416 (= S416), R423 (≠ V423)

Query Sequence

>206109 FitnessBrowser__DvH:206109
MKALSAHVFRAYDIRGIVDTDFDPEWVERLGRACGTYFVSHGHGAAVVGFDCRHSSPAYH
DALVRGLLSTGVDVTSVGMVPTPVLYFAVKHLGRKAGVMITASHNPSEYNGFKVVAGEST
IHGEEIRRIWEVFERGEFASGHGIGCSHDIVPSYIEAITSDVHPARKLKVVVDGGNGAGG
ELCVEVLRRLGVEVVAQFCEPDGDFPNHHPDPVVEANMTALMERVQVERADLGIGLDGDA
DRLGAVDGMGRLLNGDELLSLYAREMLARRPGETVIADVKCSHRLFDDIEAHGGKPMMWI
TGHSVVKARMLEVGAPLAGELSGHMFFGDRWFGFDDAIYGAARLVELLAASDVPLTDLPG
WPPSHATRELHLPCPEHAKFEVVRRAQAYFRERCTINDIDGARVIFPDGWGLVRASNTQP
VLVLRFEAQTPERLAEIRAFVEEPLRRWVAELS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory