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Comparing 206149 DVU0723 phosphoribosylglycinamide formyltransferase 2 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P33221 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; GAR transformylase T; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Escherichia coli (strain K12) (see 5 papers)
65% identity, 100% coverage: 1:392/393 of query aligns to 1:391/392 of P33221
- M1 (= M1) modified: Initiator methionine, Removed
- EL 22:23 (= EL 22:23) binding
- E82 (= E82) binding
- R114 (= R114) binding
- K155 (= K155) binding
- SSGKGQ 160:165 (= SSGKGQ 160:165) binding
- G162 (= G162) mutation to I: Strong decrease in the reaction rate for the conversion of formate to FGAR and in the affinity for formate. 3- and 2-fold decrease in the affinity for ATP and GAR, respectively.
- K179 (≠ A179) modified: N6-acetyllysine
- EGVV 195:198 (≠ EGFV 195:198) binding
- E203 (= E203) binding
- E267 (= E267) binding
- E279 (= E279) binding
- D286 (= D286) binding
- K355 (= K356) binding
- RR 362:363 (= RR 363:364) binding
1kjiA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-amppcp (see paper)
65% identity, 99% coverage: 4:392/393 of query aligns to 3:388/389 of 1kjiA
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E264 (= E267), E276 (= E279), D283 (= D286), T284 (= T287), R360 (= R364)
- binding phosphomethylphosphonic acid adenylate ester: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), Q164 (= Q165), E192 (= E195), V195 (= V198), E200 (= E203), Q222 (= Q225), E264 (= E267), F266 (= F269), E276 (= E279)
- binding magnesium ion: E264 (= E267), E276 (= E279)
1ez1A Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg, amppnp, and gar (see paper)
65% identity, 99% coverage: 4:392/393 of query aligns to 3:388/389 of 1ez1A
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E264 (= E267), E276 (= E279), D283 (= D286), T284 (= T287), R360 (= R364)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), E192 (= E195), V194 (≠ F197), V195 (= V198), F197 (= F200), E200 (= E203), Q222 (= Q225), E264 (= E267), F266 (= F269), E276 (= E279)
- binding glycinamide ribonucleotide: G20 (= G21), E21 (= E22), L22 (= L23), E81 (= E82), I82 (= I83), S160 (= S161), D283 (= D286), K352 (= K356), R359 (= R363), R360 (= R364)
- binding magnesium ion: E264 (= E267), E276 (= E279)
1eyzA Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg and amppnp (see paper)
65% identity, 99% coverage: 4:392/393 of query aligns to 3:388/389 of 1eyzA
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E264 (= E267), E276 (= E279), D283 (= D286), T284 (= T287), R360 (= R364)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), Q164 (= Q165), E192 (= E195), V195 (= V198), F197 (= F200), E200 (= E203), E264 (= E267), F266 (= F269), E276 (= E279)
- binding magnesium ion: E264 (= E267), E276 (= E279)
1kjqA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-adp (see paper)
64% identity, 99% coverage: 4:392/393 of query aligns to 3:387/388 of 1kjqA
- active site: E114 (= E115), K154 (= K155), E263 (= E267), E275 (= E279), D282 (= D286), T283 (= T287), R359 (= R364)
- binding adenosine-5'-diphosphate: R113 (= R114), I152 (≠ V153), K154 (= K155), E191 (= E195), V193 (≠ F197), V194 (= V198), F196 (= F200), E199 (= E203), Q221 (= Q225), F265 (= F269), E275 (= E279)
- binding magnesium ion: E263 (= E267), E275 (= E279)
1kjjA Crystal structure of glycniamide ribonucleotide transformylase in complex with mg-atp-gamma-s (see paper)
64% identity, 99% coverage: 4:392/393 of query aligns to 3:385/386 of 1kjjA
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E261 (= E267), E273 (= E279), D280 (= D286), T281 (= T287), R357 (= R364)
- binding phosphothiophosphoric acid-adenylate ester: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), Q164 (= Q165), E189 (= E195), V192 (= V198), E197 (= E203), Q219 (= Q225), E261 (= E267), F263 (= F269), E273 (= E279)
- binding magnesium ion: E261 (= E267), E273 (= E279)
1kj8A Crystal structure of purt-encoded glycinamide ribonucleotide transformylase in complex with mg-atp and gar (see paper)
64% identity, 99% coverage: 4:392/393 of query aligns to 3:385/386 of 1kj8A
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E261 (= E267), E273 (= E279), D280 (= D286), T281 (= T287), R357 (= R364)
- binding adenosine-5'-triphosphate: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), Q164 (= Q165), E189 (= E195), V192 (= V198), F194 (= F200), E197 (= E203), Q219 (= Q225), G222 (= G228), E261 (= E267), F263 (= F269), E273 (= E279)
- binding glycinamide ribonucleotide: G20 (= G21), E21 (= E22), L22 (= L23), E81 (= E82), I82 (= I83), S160 (= S161), D280 (= D286), K349 (= K356), R356 (= R363)
- binding magnesium ion: E261 (= E267), E273 (= E279)
O58056 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
50% identity, 99% coverage: 4:391/393 of query aligns to 8:410/430 of O58056
2dwcB Crystal structure of probable phosphoribosylglycinamide formyl transferase from pyrococcus horikoshii ot3 complexed with adp
49% identity, 98% coverage: 4:389/393 of query aligns to 10:397/409 of 2dwcB
- active site: E265 (= E267), E277 (= E279), D284 (= D286), T285 (= T287), R372 (= R364)
- binding adenosine-5'-diphosphate: R120 (= R114), H159 (≠ V153), K161 (= K155), H190 (≠ F197), I191 (≠ V198), F193 (= F200), E196 (= E203), F267 (= F269), E277 (= E279)
3q2oB Crystal structure of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
30% identity, 94% coverage: 13:383/393 of query aligns to 2:367/377 of 3q2oB
4dlkA Crystal structure of atp-ca++ complex of purk: n5- carboxyaminoimidazole ribonucleotide synthetase (see paper)
30% identity, 94% coverage: 13:383/393 of query aligns to 2:367/380 of 4dlkA
- active site: Y153 (vs. gap), G155 (= G162), E255 (= E267), E268 (= E279), N275 (≠ D286), S276 (≠ T287), K348 (≠ R364)
- binding adenosine-5'-triphosphate: E76 (= E82), F77 (≠ I83), R107 (≠ E115), K147 (= K155), Y153 (vs. gap), D154 (≠ S161), G155 (= G162), Q158 (= Q165), W184 (≠ F197), V185 (= V198), F187 (= F200), E190 (= E203), E255 (= E267), F257 (= F269), N267 (≠ S278), E268 (= E279), R272 (= R283), H274 (= H285), N275 (≠ D286), K340 (= K356), R347 (= R363), K348 (≠ R364)
- binding calcium ion: E255 (= E267), E268 (= E279)
- binding phosphate ion: Q47 (= Q51), A49 (= A53)
3v4sB Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
30% identity, 94% coverage: 13:383/393 of query aligns to 3:368/381 of 3v4sB
- binding adenosine-5'-triphosphate: R108 (≠ E115), K148 (= K155), Y154 (vs. gap), D155 (≠ S161), G156 (= G162), Q159 (= Q165), E183 (= E195), W185 (≠ F197), V186 (= V198), F188 (= F200), E191 (= E203), H214 (≠ Q225), N217 (≠ G228), E256 (= E267), F258 (= F269), E269 (= E279)
- binding carbonate ion: R273 (= R283), H275 (= H285), N276 (≠ D286)
- binding magnesium ion: T105 (≠ M112), E111 (≠ R118), E256 (= E267), E269 (= E279), L270 (≠ V280)
3v4sA Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
30% identity, 94% coverage: 13:383/393 of query aligns to 1:366/380 of 3v4sA
- binding adenosine-5'-diphosphate: R106 (≠ E115), K146 (= K155), Y152 (vs. gap), G154 (= G162), Q157 (= Q165), W183 (≠ F197), V184 (= V198), E189 (= E203), N215 (≠ G228), F256 (= F269), N266 (≠ S278), E267 (= E279)
- binding carbonate ion: R271 (= R283), H273 (= H285), N274 (≠ D286)
3r5hA Crystal structure of adp-air complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
30% identity, 94% coverage: 13:383/393 of query aligns to 2:367/383 of 3r5hA
- binding adenosine-5'-diphosphate: R107 (≠ E115), K147 (= K155), Q158 (= Q165), W184 (≠ F197), V185 (= V198), F187 (= F200), E190 (= E203), N216 (≠ G228), F257 (= F269), N267 (≠ S278), E268 (= E279)
- binding 5-aminoimidazole ribonucleotide: G17 (vs. gap), Q18 (≠ E22), L19 (= L23), E76 (= E82), Y153 (vs. gap), R272 (= R283), K340 (= K356), R347 (= R363)
3ax6A Crystal structure of n5-carboxyaminoimidazole ribonucleotide synthetase from thermotoga maritima
27% identity, 93% coverage: 27:391/393 of query aligns to 16:357/360 of 3ax6A
- active site: E231 (= E267), E244 (= E279), N251 (≠ D286), S252 (≠ T287), K330 (≠ R364)
- binding adenosine-5'-diphosphate: K101 (≠ R114), V136 (= V153), K138 (= K155), E164 (= E195), F166 (= F197), V167 (= V198), E172 (= E203), F233 (= F269), N243 (≠ S278)
3aw8A Crystal structure of n5-carboxyaminoimidazole ribonucleotide synthetase from thermus thermophilus hb8
32% identity, 78% coverage: 25:329/393 of query aligns to 12:302/360 of 3aw8A
- active site: E240 (= E267), E252 (= E279), N259 (≠ D286), S260 (≠ T287)
- binding adenosine monophosphate: L135 (≠ V153), K137 (= K155), Q142 (= Q165), F168 (= F197), V169 (= V198), E174 (= E203), H197 (≠ Q225), F242 (= F269), E252 (= E279)
Sites not aligning to the query:
5jqwA The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with adp
27% identity, 76% coverage: 14:310/393 of query aligns to 2:290/365 of 5jqwA
- active site: Y144 (≠ S160), G146 (= G162), E247 (= E267), E259 (= E279), N266 (≠ D286), S267 (≠ T287)
- binding adenosine-5'-diphosphate: R98 (= R114), K138 (= K155), G143 (≠ S159), Y144 (≠ S160), D145 (≠ S161), G146 (= G162), V178 (= V198), E183 (= E203), H206 (≠ Q225), F249 (= F269), E259 (= E279)
Sites not aligning to the query:
4ma0A The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with partially hydrolysed atp
27% identity, 76% coverage: 14:310/393 of query aligns to 2:290/366 of 4ma0A
- active site: Y144 (≠ S160), G146 (= G162), E247 (= E267), E259 (= E279), N266 (≠ D286), S267 (≠ T287)
- binding adenosine monophosphate: I136 (≠ V153), K138 (= K155), E175 (= E195), A176 (≠ G196), F177 (= F197), V178 (= V198), E183 (= E203), H206 (≠ Q225), F249 (= F269), E259 (= E279)
Sites not aligning to the query:
4mamA The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with an adp analog, amp-cp
27% identity, 76% coverage: 14:310/393 of query aligns to 2:290/373 of 4mamA
- active site: Y144 (≠ S160), G146 (= G162), E247 (= E267), E259 (= E279), N266 (≠ D286), S267 (≠ T287)
- binding phosphomethylphosphonic acid adenosyl ester: R98 (= R114), I136 (≠ V153), K138 (= K155), Y144 (≠ S160), G146 (= G162), Q149 (= Q165), E175 (= E195), F177 (= F197), V178 (= V198), F180 (= F200), E183 (= E203), H206 (≠ Q225), F249 (= F269), E259 (= E279)
Sites not aligning to the query:
3orqA Crystal structure of n5-carboxyaminoimidazole synthetase from staphylococcus aureus complexed with adp (see paper)
24% identity, 90% coverage: 22:376/393 of query aligns to 21:362/373 of 3orqA
- active site: Y156 (≠ S160), E256 (= E267), E269 (= E279), N276 (≠ D286), S277 (≠ T287), K350 (≠ R364)
- binding adenosine-5'-diphosphate: R110 (= R114), K150 (= K155), Y156 (≠ S160), D157 (≠ S161), Q161 (= Q165), E185 (= E195), Y187 (≠ F197), L188 (≠ V198), I190 (≠ F200), E193 (= E203), H216 (≠ Q225), F258 (= F269), E269 (= E279)
- binding magnesium ion: E256 (= E267), E269 (= E279)
- binding pyrophosphate 2-: Y156 (≠ S160), K342 (= K356), R349 (= R363)
Query Sequence
>206149 DVU0723 phosphoribosylglycinamide formyltransferase 2
MASIGTPLTPTATRILLLGSGELGREVALEAMRLGVEVVAVDRYPNAPAMQVAHRSHVVS
MLDGAALRAIIEAEKPYCIVPEIEAIATGTLLELEQEGYRVVPTARAARLTMDREGIRRL
AAEELGLPTSPYRFASTEEEYRAAIETVGLPCVVKPVMSSSGKGQSLVRTPADIDSAWAY
AQTGGRAGAGRVIVEGFVDFDYEITLLTVRHAGGVTFCDPIGHLQKDGDYRESWQPQPMD
AVALEKARAMADAVTGALGGWGIFGVELFVRGDEVWFSEVSPRPHDTGLVTLISQNMSEF
ALHVRAILGLPVPLLRQNGPAASCVILAEGDSEAPRFHGVDAALAEQDTALCLFGKPEVH
GRRRMGVALALGDDIDAARAKARRAAGSVTVEL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory