SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 206150 MicrobesOnline__882:206150 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

Q6LX42 Sodium/alanine symporter AgcS; Alanine permease; Alanine/glycine:cation symporter from Methanococcus maripaludis (strain S2 / LL) (see paper)
53% identity, 95% coverage: 1:444/468 of query aligns to 1:444/453 of Q6LX42

query
sites
Q6LX42

M

M

T

D

T

F

L

V

Q

S

L

L

F

V

E

N

S

T

V

V

N

N

A

S

F

F

V

V

W

W

G

G

P

P

P

Y

L

M

L

L

A

V

L

L

L

L

V

L

G

G

T

T

G

G

I

I

Y

F

L

L

T

T

I

L

R

R

L

L

G

G

L

F

L

M

Q

Q

V

I

V

H

R

T

L

L

P

P

L

Y

A

A

L

L

R

K

Y

L

V

A

F

F

G

S

R

K

D

H

D

Q

E

D

H

E

H

T

E

S

E

E

G

G

D

D

V

I

S

S

S

H

F

F

A

Q

A

A

L

L

C

M

T

T

A

A

L

L

A

A

A

A

T
|
T

I

I

G

G

T

T

G
|
G

N
|
N

I

I

V

A

G

G

V

V

A

A

T

T

A

A

V

Y

A

V

A

L

G

G

G

G

P

P

G

G

A

A

L

I

F

F

W

W

M

M

W

W

V

V

A

T

A

A

F

F

F

F

G

G

M

M

A

A

T

T

K

K

Y

Y

A

A

E

E

G

A

L

V

L

L

A

A

V

I

K

K

Y

Y

R

R

V

T

T

V

D

D

A

D

N

N

G

G

Q

E

M

M

S

A

G

G

G

G

P

P

M

M

Y

Y

Y

F

L

L

E

E

R

K

G

G

L

L

G

P

S

D

R

H

M

G

L

L

A

G

R

K

A

I

F

L

A

G

L

V

-

A

-

F

-

A

C

F

G

F

I

G

L

A

V

F

A

A

F

A

F

F

G

G

I
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I

G

G

T

N

F

M

A

V

Q
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Q

V

T

N

N

A

S

I

V

K

A

D

D

A

A

A

V

S

A

L

S

S

N

L

F

G

G

I

V

P

D

P

P

L

L

A

I

T

T

A

G

V

F

V

V

L

L

T

A

T

I

T

F

V

T

A

A

L

A

V

V

T

I

L

L

G

G

G

G

L

I

R

K

S

S

I

I

A

G

R

K

V

A

A

T

E

G

K

I

V

I

V

V

P

P

F

F

M

M

A

A

V

V

F

F

Y

Y

I

I

I

L

A

A

S

G

C

L

A

V

V

I

L

L

V

A

M

M

H

N

L

I

D

G

R

Y

I

I

P

I

D

P

A

A

V

F

M

G

V

T

I

I

L

F

H

S

S

S

A

A

F

F

N

N

P

F

Q

S

A

A

A

G

L

F

G

G

G

A

A

L

A

I

G

G

M

-

T

-

V

-

L

-

T

T

A

A

M

I

R

M

A

W

G

G

V

V

A

K

R

R

G

G

V

V

F
|
F

S
|
S

N
|
N

E
|
E

S

A

G

G

L

L

G

G

S

S

A

A

P

P

I

I

A

A

A

A

A

A

A

A

A

A

R

K

T

T

K

D

S

H

C

P

V

G

R

R

Q

Q

G

A

L

L

I

V

S

S

M

M

T

T

G

G

T

T

F

F

F

L

D
|
D

T

T

I

I

V

V

I

V

C

C

T

T

M

I

T

T

G

G

L

L

V

V

L

L

V

T

V

I

T

A

G

G

V

L

W

K

N

A

D

F

P

P

-

G

-

L

-

T

Q

D

L

L

A

T

G

G

A

A

A

S

M

L

T

T

N

A

A

A

A

S

F

F

A

D

A

A

G

L

L

M

E

P

S

-

S

-

L

M

G

G

Q

G

Y

L

A

I

A

V

T

T

L

I

G

G

L

L

I

V

L

F

F

F

A

A

F

Y

T

S

T

T

I

V

L

L

G

G

W
|
W

N

S

Y

Y

Y

Y

G

G

E

E

R

K

C

C

T

F

E

E

Y

Y

L

L

F

I

G

G

V

T

R

K

G

G

I

I

L

R

P

L

Y

Y

R

R

L

I

V

A

F

F

I

V

G

L

L

V

V

A

A

F

A

W

G

G

A

A

F

T

V

A

H

S

L

L

E

P

L

L

I

V

W

W

V

N

L

I

A

A

D

D

I

T

V

L

N

N

G

G

L

A

M

M

A

A

I

I

P

P

N

N

L

L

I

I

G

G

L

L

L

L

G

L

L

L

S

S

G

G

V

V

I

V

I

V

A

S

E

E

T

T

R

K

A

A

F

F

F

N

E

E

T75 (= T75) binding ; binding
G79 (= G79) binding
N80 (= N80) binding ; mutation to A: Markedly reduces L-alanine uptake; when associated with A-274 and A-308.
I165 (= I162) mutation to A: Shows poor substrate selectivity, allowing additional amino acids such as L-valine and L-leucine to be efficiently transported. Decreases L-alanine uptake.
Q170 (= Q167) binding ; binding
F273 (= F274) mutation to A: Shows poor substrate selectivity, allowing additional amino acids such as L-valine and L-leucine to be efficiently transported. Decreases L-alanine uptake.
FS 273:274 (= FS 274:275) binding
FSNE 273:276 (= FSNE 274:277) binding
S274 (= S275) mutation to A: Markedly reduces L-alanine uptake; when associated with A-80 and A-308.
D308 (= D309) mutation to A: Markedly reduces L-alanine uptake; when associated with A-80 and A-274.
W370 (= W370) mutation to Q: Does not change uptake of L-alanine, but reduces the ability to transport D-alanine.

6csfM Crystal structure of sodium/alanine symporter agcs with d-alanine bound (see paper)
54% identity, 92% coverage: 15:444/468 of query aligns to 1:424/431 of 6csfM

query
sites
6csfM

W

W

G

G

P

P

P

Y

L

M

L

L

A

V

L

L

L

L

V

L

G

G

T

T

G

G

I

I

Y

F

L

L

T

T

I

L

R

R

L

L

G

G

L

F

L

M

Q

Q

V

I

V

H

R

T

L

L

P

P

L

Y

A

A

L

L

R

K

Y

L

V

A

F

F

G

S

R

K

D

E

D

T

E

S

H

-

H

-

E

-

E

E

G

G

D

D

V

I

S

S

S

H

F

F

A

Q

A

A

L

L

C

M

T

T

A

A

L

L

A

A

A

A

T
|
T

I
|
I

G
|
G

T

T

G
|
G

N
|
N

I

I

V

A

G

G

V

V

A

A

T

T

A

A

V

Y

A

V

A

L

G

G

G

G

P

P

G

G

A

A

L

I

F

F

W

W

M

M

W

W

V

V

A

T

A

A

F

F

F

F

G

G

M

M

A

A

T

T

K

K

Y

Y

A

A

E

E

G

A

L

V

L

L

A

A

V

I

K

K

Y

Y

R

R

V

T

T

V

D

D

A

D

N

N

G

G

Q

E

M

M

S

A

G

G

G

G

P

P

M

M

Y

Y

Y

F

L

L

E

E

R

K

G

G

L

L

G

P

S

L

R

G

M

K

L

I

A

L

R

G

A

V

F

A

A

F

L

A

C

F

G

F

I

G

L

A

V

F

A

A

F

A

F

F

G

G

I

I

G

G

T

N

F

M

A

V

Q
|
Q

V

T

N

N

A

S

I

V

K

A

D

D

A

A

A

V

S

A

L

S

S

N

L

F

G

G

I

V

P

D

P

P

L

L

A

I

T

T

A

G

V

F

V

V

L

L

T

A

T

I

T

F

V

T

A

A

L

A

V

V

T

I

L

L

G

G

G

G

L

I

R

K

S

S

I

I

A

G

R

K

V

A

A

T

E

G

K

I

V

I

V

V

P

P

F

F

M

M

A

A

V

V

F

F

Y

Y

I

I

I

L

A

A

S

G

C

L

A

V

V

I

L

L

V

A

M

M

H

N

L

I

D

G

R

Y

I

I

P

I

D

P

A

A

V

F

M

G

V

T

I

I

L

F

H

S

S

S

A

A

F

F

N

N

P

F

Q

S

A

A

A

G

L

F

G

G

G

A

A

L

A

I

G

G

M

-

T

-

V

-

L

-

T

T

A

A

M

I

R

M

A

W

G

G

V

V

A

K

R

R

G

G

V

V

F
|
F

S
|
S

N

N

E

E

S

A

G

G

L

L

G

G

S

S

A

A

P

P

I

I

A

A

A

A

A

A

A

A

A

A

R

K

T

T

K

D

S

H

C

P

V

G

R

R

Q

Q

G

A

L

L

I

V

S

S

M

M

T

T

G

G

T

T

F

F

F

L

D
|
D

T

T

I

I

V

V

I

V

C

C

T

T

M

I

T

T

G

G

L

L

V

V

L

L

V

T

V

I

T

A

G

G

V

L

W

K

N

A

D

F

P

P

-

G

-

L

-

T

Q

D

L

L

A

T

G

G

A

A

A

S

M

L

T

T

N

A

A

A

A

S

F

F

A

D

A

A

G

L

L

M

E

P

S

-

S

-

L

M

G

G

Q

G

Y

L

A

I

A

V

T

T

L

I

G

G

L

L

I

V

L

F

F

F

A

A

F

Y

T

S

T

T

I

V

L

L

G

G

W

W

N

S

Y

Y

Y

Y

G

G

E

E

R

K

C

C

T

F

E

E

Y

Y

L

L

F

I

G

G

V

T

R

K

G

G

I

I

L

R

P

L

Y

Y

R

R

L

I

V

A

F

F

I

V

G

L

L

V

V

A

A

F

A

W

G

G

A

A

F

T

V

A

H

S

L

L

E

P

L

L

I

V

W

W

V

N

L

I

A

A

D

D

I

T

V

L

N

N

G

G

L

A

M

M

A

A

I

I

P

P

N

N

L

L

I

I

G

G

L

L

L

L

G

L

L

L

S

S

G

G

V

V

I

V

I

V

A

S

E

E

T

T

R

K

A

A

F

F

F

N

E

E

binding d-alanine: T58 (= T75), G60 (= G77), G62 (= G79), N63 (= N80), Q150 (= Q167), F253 (= F274), S254 (= S275)
binding sodium ion: T58 (= T75), I59 (= I76), N63 (= N80), S254 (= S275), D288 (= D309)

6cseM Crystal structure of sodium/alanine symporter agcs with l-alanine bound (see paper)
54% identity, 92% coverage: 15:444/468 of query aligns to 1:424/431 of 6cseM

query
sites
6cseM

W

W

G

G

P

P

P

Y

L

M

L

L

A

V

L

L

L

L

V

L

G

G

T

T

G

G

I

I

Y

F

L

L

T

T

I

L

R

R

L

L

G

G

L

F

L

M

Q

Q

V

I

V

H

R

T

L

L

P

P

L

Y

A

A

L

L

R

K

Y

L

V

A

F

F

G

S

R

K

D

E

D

T

E

S

H

-

H

-

E

-

E

E

G

G

D

D

V

I

S

S

S

H

F

F

A

Q

A

A

L

L

C

M

T

T

A

A

L

L

A

A

A
|
A

T
|
T

I
|
I

G
|
G

T

T

G

G

N
|
N

I

I

V

A

G

G

V

V

A

A

T

T

A

A

V

Y

A

V

A

L

G

G

G

G

P

P

G

G

A

A

L

I

F

F

W

W

M

M

W

W

V

V

A

T

A

A

F

F

F

F

G

G

M

M

A

A

T

T

K

K

Y

Y

A

A

E

E

G

A

L

V

L

L

A

A

V

I

K

K

Y

Y

R

R

V

T

T

V

D

D

A

D

N

N

G

G

Q

E

M

M

S

A

G

G

G

G

P

P

M

M

Y

Y

Y

F

L

L

E

E

R

K

G

G

L

L

G

P

S

L

R

G

M

K

L

I

A

L

R

G

A

V

F

A

A

F

L

A

C

F

G

F

I

G

L

A

V

F

A

A

F

A

F

F

G

G

I

I

G

G

T

N

F

M

A

V

Q
|
Q

V

T

N

N

A

S

I

V

K

A

D

D

A

A

A

V

S

A

L

S

S

N

L

F

G

G

I

V

P

D

P

P

L

L

A

I

T

T

A

G

V

F

V

V

L

L

T

A

T

I

T

F

V

T

A

A

L

A

V

V

T

I

L

L

G

G

G

G

L

I

R

K

S

S

I

I

A

G

R

K

V

A

A

T

E

G

K

I

V

I

V

V

P

P

F

F

M

M

A

A

V

V

F

F

Y

Y

I

I

I

L

A

A

S

G

C

L

A

V

V

I

L

L

V

A

M

M

H

N

L

I

D

G

R

Y

I

I

P

I

D

P

A

A

V

F

M

G

V

T

I

I

L

F

H

S

S

S

A

A

F

F

N

N

P

F

Q

S

A

A

A

G

L

F

G

G

G

A

A

L

A

I

G

G

M

-

T

-

V

-

L

-

T

T

A

A

M

I

R

M

A

W

G

G

V

V

A

K

R

R

G

G

V

V

F
|
F

S
|
S

N

N

E
|
E

S

A

G

G

L

L

G

G

S

S

A

A

P

P

I

I

A

A

A

A

A

A

A

A

A

A

R

K

T

T

K

D

S

H

C

P

V

G

R

R

Q

Q

G

A

L

L

I

V

S

S

M

M

T

T

G

G

T

T

F

F

F

L

D
|
D

T

T

I

I

V

V

I

V

C

C

T

T

M

I

T

T

G

G

L

L

V

V

L

L

V

T

V

I

T

A

G

G

V

L

W

K

N

A

D

F

P

P

-

G

-

L

-

T

Q

D

L

L

A

T

G

G

A

A

A

S

M

L

T

T

N

A

A

A

A

S

F

F

A

D

A

A

G

L

L

M

E

P

S

-

S

-

L

M

G

G

Q

G

Y

L

A

I

A

V

T

T

L

I

G

G

L

L

I

V

L

F

F

F

A

A

F

Y

T

S

T

T

I

V

L

L

G

G

W

W

N

S

Y

Y

Y

Y

G

G

E

E

R

K

C

C

T

F

E

E

Y

Y

L

L

F

I

G

G

V

T

R

K

G

G

I

I

L

R

P

L

Y

Y

R

R

L

I

V

A

F

F

I

V

G

L

L

V

V

A

A

F

A

W

G

G

A

A

F

T

V

A

H

S

L

L

E

P

L

L

I

V

W

W

V

N

L

I

A

A

D

D

I

T

V

L

N

N

G

G

L

A

M

M

A

A

I

I

P

P

N

N

L

L

I

I

G

G

L

L

L

L

G

L

L

L

S

S

G

G

V

V

I

V

I

V

A

S

E

E

T

T

R

K

A

A

F

F

F

N

E

E

binding alanine: A57 (= A74), T58 (= T75), G60 (= G77), N63 (= N80), Q150 (= Q167), F253 (= F274), S254 (= S275), E256 (= E277)
binding sodium ion: T58 (= T75), I59 (= I76), N63 (= N80), S254 (= S275), D288 (= D309)

Query Sequence

>206150 MicrobesOnline__882:206150
MTTLQLFESVNAFVWGPPLLALLVGTGIYLTIRLGLLQVVRLPLALRYVFGRDDEHHEEG
DVSSFAALCTALAATIGTGNIVGVATAVAAGGPGALFWMWVAAFFGMATKYAEGLLAVKY
RVTDANGQMSGGPMYYLERGLGSRMLARAFALCGILVAFFGIGTFAQVNAIKDAASLSLG
IPPLATAVVLTTTVALVTLGGLRSIARVAEKVVPFMAVFYIIASCAVLVMHLDRIPDAVM
VILHSAFNPQAALGGAAGMTVLTAMRAGVARGVFSNESGLGSAPIAAAAARTKSCVRQGL
ISMTGTFFDTIVICTMTGLVLVVTGVWNDPQLAGAAMTNAAFAAGLESSLGQYAATLGLI
LFAFTTILGWNYYGERCTEYLFGVRGILPYRLVFIGLVAAGAFVHLELIWVLADIVNGLM
AIPNLIGLLGLSGVIIAETRAFFEADAPSVDAPCGSDADNASGACPRA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory