SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 206178 MicrobesOnline__882:206178 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4zv1A An ancestral arginine-binding protein bound to arginine (see paper)
41% identity, 81% coverage: 36:258/275 of query aligns to 6:226/226 of 4zv1A

query
sites
4zv1A

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binding arginine: E11 (≠ M41), F14 (≠ S44), F52 (≠ N82), A69 (≠ S99), G70 (≠ N100), M71 (= M101), T72 (= T102), R77 (= R107), Q117 (≠ M145), S120 (≠ T148), T121 (= T149), D161 (= D194)

4zv2A An ancestral arginine-binding protein bound to glutamine (see paper)
41% identity, 81% coverage: 36:258/275 of query aligns to 6:224/225 of 4zv2A

query
sites
4zv2A

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binding glutamine: E11 (≠ M41), F14 (≠ S44), F52 (≠ N82), A69 (≠ S99), G70 (≠ N100), M71 (= M101), T72 (= T102), R77 (= R107), S120 (≠ T148), T121 (= T149), D159 (= D194)

5eyfB Crystal structure of solute-binding protein from enterococcus faecium with bound glutamate
40% identity, 73% coverage: 58:257/275 of query aligns to 38:234/243 of 5eyfB

query
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5eyfB

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binding glutamic acid: S65 (≠ N82), R68 (= R85), T83 (≠ N100), M84 (= M101), T85 (= T102), R90 (= R107), G132 (= G147), S133 (≠ T148), T134 (= T149), Y154 (≠ E176), T171 (= T193), D172 (= D194), E196 (= E219)

Sites not aligning to the query:

binding glutamic acid: 20

5t0wA Crystal structure of the ancestral amino acid-binding protein anccdt- 1, a precursor of cyclohexadienyl dehydratase
36% identity, 84% coverage: 28:257/275 of query aligns to 4:228/229 of 5t0wA

query
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5t0wA

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binding arginine: D17 (≠ M41), Y20 (≠ S44), W58 (≠ N82), S75 (= S99), G76 (≠ N100), M77 (= M101), T78 (= T102), R83 (= R107), Q126 (≠ M145), T129 (= T148), T130 (= T149), D168 (= D194)

6svfA Crystal structure of the p235gk mutant of argbp from t. Maritima (see paper)
32% identity, 84% coverage: 26:257/275 of query aligns to 2:227/229 of 6svfA

query
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6svfA

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binding arginine: S17 (≠ M41), D19 (= D43), F20 (≠ S44), E24 (≠ G48), F58 (≠ N82), S75 (= S99), G76 (≠ N100), M77 (= M101), T78 (= T102), R83 (= R107), Q124 (≠ M145), T127 (= T148), T128 (= T149), D165 (= D194)

6h2tA Glnh bound to glu, mycobacterium tuberculosis (see paper)
34% identity, 77% coverage: 54:266/275 of query aligns to 73:281/288 of 6h2tA

query
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6h2tA

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S

H

I

T

T

R

C

E

E

R
|
R

D

R

K

K

S

L

V

V

D

N

F

F

S

S

I

T

T

V

Y

Y

F

L

F

D

D

A

G

N

Q

Q

K

R

V

I

L

L

A

A

K

P

K

R

-

D

G

S

Q

P

F

I

K

T

S

K

V

V

K

S

D

D

M

L

V

S

G

G

K

K

K

R

I

V

A

C

T

V

M

A

Q

R

G

G

T
|
T

S
|
S

E

-

V

-

N

-

V

-

K

L

R

R

A

R

L

I

K

R

E

E

A

I

G

A

D

P

P

P

N

-

P

-

D

-

Q

P

S

V

V

I

I

V

S

S

F

V

Q

V

K

N

E
x
W

S

A

E

D

C

C

F

L

Q

V

A

A

L

L

Q

Q

M

Q

G

R

R

E

V

I

A

D

G

A

W

V

S

S

T
|
T

D
|
D

A

D

S

T

I
|
I

L

L

V

A

G

G

Y

L

S

V

A

E

K

E

E

D

P

P

G

-

K

Y

F

L

E

H

L

I

V

V

G

G

N

P

F

D

L

M

A

A

E

D

E
x
Q

P

P

Y

Y

G

G

I

V

A

G

M

I

P

N

Q

L

D

D

D

N

S

T

A

G

L

L

R

V

D

R

A

F

V

V

N

N

A

G

A

T

L

L

Q

E

D

R

M

I

W

R

K

N

D

D

G

G

T

T

Y

W

K

N

T

T

I

L

Y

Y

N

R

K

K

W

W

Y

L

G

T

P

V

G

L

T

G

P

P

F

A

E

P

M

A

P

P

binding glutamic acid: R107 (= R85), T122 (≠ N100), M123 (= M101), S124 (≠ T102), R129 (= R107), T171 (= T148), T172 (= T149), S173 (= S150), W192 (≠ E176), T209 (= T193), D210 (= D194), I213 (= I197), Q234 (≠ E219)

6h20A Glnh bound to asn, mycobacterium tuberculosis (see paper)
35% identity, 77% coverage: 54:266/275 of query aligns to 72:280/287 of 6h20A

query
sites
6h20A

G

G

E

E

W

I

G

T

G

G

F

F

D

D

Y

V

D

D

I

I

A

A

T

G

E

E

I

V

A

A

K

R

R

D

M

I

-

F

G

G

V

V

-

P

-

S

D

H

I

V

E

E

R

Y

V

R

Q

I

V

L

N

S

N

A

K

A

T

E

R
|
R

I

V

A

T

Y

A

V

L

Q

Q

Q

K

G

S

R

Q

V

V

D

D

I

I

S

V

V

V

S

K

N
x
T

M
|
M

T
x
S

H

I

T

T

R

C

E

E

R
|
R

D

R

K

K

S

L

V

V

D

N

F

F

S

S

I

T

T

V

Y

Y

F

L

F

D

D

A

G

N

Q

Q

K

R

V

I

L

L

A

A

K

P

K

R

-

D

G

S

Q

P

F

I

K

T

S

K

V

V

K

S

D

D

M

L

V

S

G

G

K

K

K

R

I

V

A

C

T

V

M

A

Q

R

G

G

T
|
T

S

S

E

-

V

-

N

-

V

-

K

L

R

R

A

R

L

I

K

R

E

E

A

I

G

A

D

P

P

P

N

P

P

V

D

I

Q

V

S

S

V

V

I

V

S

N

F
x
W

Q

-

K

-

E

-

S

A

E

D

C

C

F

L

Q

V

A

A

L

L

Q

Q

M

Q

G

R

R

E

V

I

A

D

G

A

W

V

S

S

T

T

D
|
D

A

D

S

T

I
|
I

L

L

V

A

G

G

Y

L

S

V

A

E

K

E

E

D

P

P

G

-

K

Y

F

L

E

H

L

I

V

V

G

G

N

P

F

D

L

M

A

A

E

D

E
x
Q

P

P

Y

Y

G

G

I

V

A

G

M

I

P

N

Q

L

D

D

D

N

S

T

A

G

L

L

R

V

D

R

A

F

V

V

N

N

A

G

A

T

L

L

Q

E

D

R

M

I

W

R

K

N

D

D

G

G

T

T

Y

W

K

N

T

T

I

L

Y

Y

N

R

K

K

W

W

Y

L

G

T

P

V

G

L

T

G

P

P

F

A

E

P

M

A

P

P

binding asparagine: R106 (= R85), T121 (≠ N100), M122 (= M101), S123 (≠ T102), R128 (= R107), T170 (= T148), T171 (= T149), W191 (≠ F173), D209 (= D194), I212 (= I197), Q233 (≠ E219)

6h1uA Glnh bound to asp, mycobacterium tuberculosis (see paper)
35% identity, 77% coverage: 54:266/275 of query aligns to 72:280/287 of 6h1uA

query
sites
6h1uA

G

G

E

E

W

I

G

T

G

G

F

F

D

D

Y

V

D

D

I

I

A

A

T

G

E

E

I

V

A

A

K

R

R

D

M

I

-

F

G

G

V

V

-

P

-

S

D

H

I

V

E

E

R

Y

V

R

Q

I

V

L

N

S

N

A

K

A

T

E

R
|
R

I

V

A

T

Y

A

V

L

Q

Q

Q

K

G

S

R

Q

V

V

D

D

I

I

S

V

V

V

S

K

N
x
T

M
|
M

T
x
S

H

I

T

T

R

C

E

E

R
|
R

D

R

K

K

S

L

V

V

D

N

F

F

S

S

I

T

T

V

Y

Y

F

L

F

D

D

A

G

N

Q

Q

K

R

V

I

L

L

A

A

K

P

K

R

-

D

G

S

Q

P

F

I

K

T

S

K

V

V

K

S

D

D

M

L

V

S

G

G

K

K

K

R

I

V

A

C

T

V

M

A

Q

R

G

G

T
|
T

S

S

E

-

V

-

N

-

V

-

K

L

R

R

A

R

L

I

K

R

E

E

A

I

G

A

D

P

P

P

N

P

P

V

D

I

Q

V

S

S

V

V

I

V

S

N

F
x
W

Q

-

K

-

E

-

S

A

E

D

C

C

F

L

Q

V

A

A

L

L

Q

Q

M

Q

G

R

R

E

V

I

A

D

G

A

W

V

S

S

T

T

D
|
D

A

D

S

T

I
|
I

L

L

V

A

G

G

Y

L

S

V

A

E

K

E

E

D

P

P

G

-

K

Y

F

L

E

H

L

I

V

V

G

G

N

P

F

D

L

M

A

A

E

D

E
x
Q

P

P

Y

Y

G

G

I

V

A

G

M

I

P

N

Q

L

D

D

D

N

S

T

A

G

L

L

R

V

D

R

A

F

V

V

N

N

A

G

A

T

L

L

Q

E

D

R

M

I

W

R

K

N

D

D

G

G

T

T

Y

W

K

N

T

T

I

L

Y

Y

N

R

K

K

W

W

Y

L

G

T

P

V

G

L

T

G

P

P

F

A

E

P

M

A

P

P

binding aspartic acid: R106 (= R85), T121 (≠ N100), M122 (= M101), S123 (≠ T102), R128 (= R107), T170 (= T148), T171 (= T149), W191 (≠ F173), D209 (= D194), I212 (= I197), Q233 (≠ E219)

3vv5A Crystal structure of ttc0807 complexed with (s)-2-aminoethyl-l- cysteine (aec) (see paper)
28% identity, 85% coverage: 26:258/275 of query aligns to 5:231/237 of 3vv5A

query
sites
3vv5A

A

S

Y

F

D

E

N

E

V

I

M

K

K

R

D

S

K

G

K

E

I

I

R

R

V

I

G

G

L

T

M
x
E

T

G

D

A

S
x
F

I

P

P

P

G

F

G
x
N

F

Y

Y

F

N

D

E

E

K

R

G

N

E

Q

W

L

G

T

G

G

F

F

D

E

Y

V

D

D

I

L

A

G

T

N

E

A

I

I

A

A

K

E

R

R

M

L

G

G

V

L

D

K

I

P

E

R

R

W

V

I

Q

A

V

Q

N

S

N
x
F

K

D

T

T

R

L

I

L

A

I

Y

Q

V

L

Q

N

Q

Q

G

G

R

R

V

F

D

D

I

F

S

V

V

I

S
x
A

N
x
S

M
x
H

T
x
G

H

I

T

T

R

E

E

E

R
|
R

D

A

K

R

S

A

V

V

D

D

F

F

S

T

I

N

T

P

Y

H

F

Y

F

C

D

T

G

G

Q

G

K

V

V

I

L

V

A

S

K

R

K

K

G

G

Q

G

F

P

K

R

S

T

V

A

K

K

D

D

M

L

V

Q

G

G

K

K

K

V

I

V

A

G

T

V

M
x
Q

Q

V

G

G

T
|
T

S

-

E

-

V

-

N

-

V

-

K

-

R

-

A

-

L
x
Y

K

M

E

E

A

A

G

A

D

Q

P

K

N

I

P

P

D

G

-

I

Q

K

S

E

V

V

I

R

S

T

F

Y

Q

Q

K

R

E

D

S

P

E

D

C

A

F

L

Q

Q

A

D

L

L

Q

L

M

A

G

G

R

R

V

I

A

D

G

T

W

W

S

I

T

T

D

D

A

R

S

-

I

-

L

F

V

V

G

A

Y

K

S

E

A

A

K

I

E

K

P

E

G

R

K

K

F

L

E

E

-

N

-

T

-

L

L

Q

V

V

G

G

N

E

F

L

L

V

A

F

E

Q

E
|
E

P

R

Y

V

G

A

I

M

A

A

M

V

P

A

Q

K

D

G

D

N

S

K

A

S

L

L

R

L

D

D

A

A

V

L

N

N

A

R

A

A

L

L

Q

A

D

E

M

L

W

M

K

Q

D

D

G

G

T

T

Y

Y

K

A

T

R

I

I

Y

S

N

Q

K

K

W

W

Y

F

G

G

binding l-thialysine: E20 (≠ M41), F23 (≠ S44), N27 (≠ G48), F61 (≠ N82), A78 (≠ S99), S79 (≠ N100), H80 (≠ M101), G81 (≠ T102), R86 (= R107), Q124 (≠ M145), T127 (= T148), T128 (= T149), Y129 (≠ L158), E192 (= E219)

3vvfA Crystal structure of ttc0807 complexed with arginine (see paper)
28% identity, 85% coverage: 26:258/275 of query aligns to 9:235/241 of 3vvfA

query
sites
3vvfA

A

S

Y

F

D

E

N

E

V

I

M

K

K

R

D

S

K

G

K

E

I

I

R

R

V

I

G

G

L

T

M
x
E

T

G

D

A

S
x
F

I

P

P

P

G

F

G
x
N

F

Y

Y

F

N

D

E

E

K

R

G

N

E

Q

W

L

G

T

G

G

F

F

D

E

Y

V

D

D

I

L

A

G

T

N

E

A

I

I

A

A

K

E

R

R

M

L

G

G

V

L

D

K

I

P

E

R

R

W

V

I

Q

A

V

Q

N

S

N
x
F

K

D

T

T

R

L

I

L

A

I

Y

Q

V

L

Q

N

Q

Q

G

G

R

R

V

F

D

D

I

F

S

V

V

I

S
x
A

N
x
S

M
x
H

T
x
G

H

I

T

T

R

E

E

E

R
|
R

D

A

K

R

S

A

V

V

D

D

F

F

S

T

I

N

T

P

Y

H

F

Y

F

C

D

T

G

G

Q

G

K

V

V

I

L

V

A

S

K

R

K

K

G

G

Q

G

F

P

K

R

S

T

V

A

K

K

D

D

M

L

V

Q

G

G

K

K

K

V

I

V

A

G

T

V

M
x
Q

Q

V

G

G

T
|
T

S

-

E

-

V

-

N

-

V

-

K

-

R

-

A

-

L
x
Y

K

M

E

E

A

A

G

A

D

Q

P

K

N

I

P

P

D

G

-

I

Q

K

S

E

V

V

I

R

S

T

F

Y

Q

Q

K

R

E

D

S

P

E

D

C

A

F

L

Q

Q

A

D

L

L

Q

L

M

A

G

G

R

R

V

I

A

D

G

T

W

W

S

I

T

T

D

D

A

R

S

-

I

-

L

F

V

V

G

A

Y

K

S

E

A

A

K

I

E

K

P

E

G

R

K

K

F

L

E

E

-

N

-

T

-

L

L

Q

V

V

G

G

N

E

F

L

L

V

A

F

E

Q

E
|
E

P

R

Y

V

G

A

I

M

A

A

M

V

P

A

Q

K

D

G

D

N

S

K

A

S

L

L

R

L

D

D

A

A

V

L

N

N

A

R

A

A

L

L

Q

A

D

E

M

L

W

M

K

Q

D

D

G

G

T

T

Y

Y

K

A

T

R

I

I

Y

S

N

Q

K

K

W

W

Y

F

G

G

binding arginine: E24 (≠ M41), F27 (≠ S44), N31 (≠ G48), F65 (≠ N82), A82 (≠ S99), S83 (≠ N100), H84 (≠ M101), G85 (≠ T102), R90 (= R107), Q128 (≠ M145), T131 (= T148), T132 (= T149), Y133 (≠ L158), E196 (= E219)

3vveA Crystal structure of ttc0807 complexed with lysine (see paper)
28% identity, 85% coverage: 26:258/275 of query aligns to 9:235/241 of 3vveA

query
sites
3vveA

A

S

Y

F

D

E

N

E

V

I

M

K

K

R

D

S

K

G

K

E

I

I

R

R

V

I

G

G

L

T

M
x
E

T

G

D

A

S
x
F

I

P

P

P

G

F

G

N

F

Y

Y

F

N

D

E

E

K

R

G

N

E

Q

W

L

G

T

G

G

F

F

D

E

Y

V

D

D

I

L

A

G

T

N

E

A

I

I

A

A

K

E

R

R

M

L

G

G

V

L

D

K

I

P

E

R

R

W

V

I

Q

A

V

Q

N

S

N
x
F

K

D

T

T

R

L

I

L

A

I

Y

Q

V

L

Q

N

Q

Q

G

G

R

R

V

F

D

D

I

F

S

V

V

I

S
x
A

N
x
S

M
x
H

T
x
G

H

I

T

T

R

E

E

E

R
|
R

D

A

K

R

S

A

V

V

D

D

F

F

S

T

I

N

T

P

Y

H

F

Y

F

C

D

T

G

G

Q

G

K

V

V

I

L

V

A

S

K

R

K

K

G

G

Q

G

F

P

K

R

S

T

V

A

K

K

D

D

M

L

V

Q

G

G

K

K

K

V

I

V

A

G

T

V

M
x
Q

Q

V

G

G

T
|
T

S

-

E

-

V

-

N

-

V

-

K

-

R

-

A

-

L
x
Y

K

M

E

E

A

A

G

A

D

Q

P

K

N

I

P

P

D

G

-

I

Q

K

S

E

V

V

I

R

S

T

F

Y

Q

Q

K

R

E

D

S

P

E

D

C

A

F

L

Q

Q

A

D

L

L

Q

L

M

A

G

G

R

R

V

I

A

D

G

T

W

W

S

I

T

T

D

D

A

R

S

-

I

-

L

F

V

V

G

A

Y

K

S

E

A

A

K

I

E

K

P

E

G

R

K

K

F

L

E

E

-

N

-

T

-

L

L

Q

V

V

G

G

N

E

F

L

L

V

A

F

E

Q

E
|
E

P

R

Y

V

G

A

I

M

A

A

M

V

P

A

Q

K

D

G

D

N

S

K

A

S

L

L

R

L

D

D

A

A

V

L

N

N

A

R

A

A

L

L

Q

A

D

E

M

L

W

M

K

Q

D

D

G

G

T

T

Y

Y

K

A

T

R

I

I

Y

S

N

Q

K

K

W

W

Y

F

G

G

binding lysine: E24 (≠ M41), F27 (≠ S44), F65 (≠ N82), A82 (≠ S99), S83 (≠ N100), H84 (≠ M101), G85 (≠ T102), R90 (= R107), Q128 (≠ M145), T131 (= T148), T132 (= T149), Y133 (≠ L158), E196 (= E219)

3vvdA Crystal structure of ttc0807 complexed with ornithine (see paper)
28% identity, 85% coverage: 26:258/275 of query aligns to 9:235/241 of 3vvdA

query
sites
3vvdA

A

S

Y

F

D

E

N

E

V

I

M

K

K

R

D

S

K

G

K

E

I

I

R

R

V

I

G

G

L

T

M
x
E

T

G

D

A

S
x
F

I

P

P

P

G

F

G

N

F

Y

Y

F

N

D

E

E

K

R

G

N

E

Q

W

L

G

T

G

G

F

F

D

E

Y

V

D

D

I

L

A

G

T

N

E

A

I

I

A

A

K

E

R

R

M

L

G

G

V

L

D

K

I

P

E

R

R

W

V

I

Q

A

V

Q

N

S

N
x
F

K

D

T

T

R

L

I

L

A

I

Y

Q

V

L

Q

N

Q

Q

G

G

R

R

V

F

D

D

I

F

S

V

V

I

S

A

N
x
S

M
x
H

T
x
G

H

I

T

T

R

E

E

E

R
|
R

D

A

K

R

S

A

V

V

D

D

F

F

S

T

I

N

T

P

Y

H

F

Y

F

C

D

T

G

G

Q

G

K

V

V

I

L

V

A

S

K

R

K

K

G

G

Q

G

F

P

K

R

S

T

V

A

K

K

D

D

M

L

V

Q

G

G

K

K

K

V

I

V

A

G

T

V

M
x
Q

Q

V

G

G

T
|
T

S

-

E

-

V

-

N

-

V

-

K

-

R

-

A

-

L
x
Y

K

M

E

E

A

A

G

A

D

Q

P

K

N

I

P

P

D

G

-

I

Q

K

S

E

V

V

I

R

S

T

F

Y

Q

Q

K

R

E

D

S

P

E

D

C

A

F

L

Q

Q

A

D

L

L

Q

L

M

A

G

G

R

R

V

I

A

D

G

T

W

W

S

I

T

T

D

D

A

R

S

-

I

-

L

F

V

V

G

A

Y

K

S

E

A

A

K

I

E

K

P

E

G

R

K

K

F

L

E

E

-

N

-

T

-

L

L

Q

V

V

G

G

N

E

F

L

L

V

A

F

E

Q

E
|
E

P

R

Y

V

G

A

I

M

A

A

M

V

P

A

Q

K

D

G

D

N

S

K

A

S

L

L

R

L

D

D

A

A

V

L

N

N

A

R

A

A

L

L

Q

A

D

E

M

L

W

M

K

Q

D

D

G

G

T

T

Y

Y

K

A

T

R

I

I

Y

S

N

Q

K

K

W

W

Y

F

G

G

binding L-ornithine: E24 (≠ M41), F27 (≠ S44), F65 (≠ N82), S83 (≠ N100), H84 (≠ M101), G85 (≠ T102), R90 (= R107), Q128 (≠ M145), T131 (= T148), T132 (= T149), Y133 (≠ L158), E196 (= E219)

2pyyB Crystal structure of the glur0 ligand-binding core from nostoc punctiforme in complex with (l)-glutamate (see paper)
30% identity, 76% coverage: 57:264/275 of query aligns to 18:217/217 of 2pyyB

query
sites
2pyyB

G

S

G

G

F

F

D

S

Y

I

D

D

I

L

A

W

T

R

E

S

I

I

A

A

K

T

R

Q

M

I

G

G

V

I

D

E

I

S

E

K

R

L

V

I

Q

E

V

Y

N

S

K
x
V

T

P

R

E

-

L

I

I

A

S

Y

A

V

I

Q

K

Q

D

G

N

R

K

V

V

D

N

I

L

S

G

V

I

S

A

N
x
A

M
x
I

T
x
S

H

I

T

T

R

A

E

E

R
|
R

D

E

K

Q

S

N

V

F

D

D

F

F

S

S

I

L

T

P

Y

I

F

F

F

A

D

S

G

G

Q

L

K

Q

V

I

L

M

A

V

K

R

K

N

G

G

Q

D

F

I

K

R

S

S

V

I

K

D

D

D

M

L

V

P

G

G

K

K

K

V

I

V

A

A

T

T

M
x
T

Q

A

G

G

T
x
S

T
|
T

S

A

E

A

V

T

N

Y

V

L

K

R

R

E

A

H

L

-

K

-

E

-

A

-

G

-

D

-

P

-

N

-

P

-

D

H

Q

I

S

S

V

V

I

L

S

E

F

V

Q

P

K

K

E

I

S

E

E

E

C

A

F

Y

Q

K

A

A

L

L

Q

Q

M

T

G

K

R

K

V

A

A

D

G

A

W

V

S

V

T
x
F

D
|
D

A

A

S

P

I

V

L

L

V

L

G

F

Y

Y

S

A

A

A

K

N

E

E

-

G

P

K

G

G

K

K

F

V

E

E

L

I

V

V

G

G

N

S

F

I

L

L

A

R

E

E

P

S

Y

Y

G

G

I

I

A

I

M

L

P

P

Q

-

D

N

S

S

A

P

L

Y

R

R

D

K

A

P

V

I

N

N

A

Q

A

A

L

L

Q

L

D

N

M

L

W

K

K

E

D

N

G

G

T

T

Y

Y

K

Q

T

S

I

L

Y

Y

N

D

K

K

W

W

Y

F

G

D

P

P

G

K

T

N

P

S

F

L

E

E

binding glutamic acid: V44 (≠ K83), A62 (≠ N100), I63 (≠ M101), S64 (≠ T102), R69 (= R107), T107 (≠ M145), S110 (≠ T148), T111 (= T149), F146 (≠ T193), D147 (= D194)

Sites not aligning to the query:

binding glutamic acid: 7

8eyzA Engineered glutamine binding protein bound to gln and a cobaloxime ligand (see paper)
33% identity, 82% coverage: 33:258/275 of query aligns to 2:222/226 of 8eyzA

query
sites
8eyzA

D

D

K

K

I

L

R

V

V

V

G

A

L

T

M
x
D

T

T

D

A

S
x
F

I

V

P

P

G

F

G

E

F

F

Y

-

N

-

E

K

K

Q

G

G

E

D

-

I

W

Y

G

V

G

G

F

F

D

D

Y

V

D

D

I

L

A

W

T

A

E

A

I

I

A

A

K

K

R

E

M

L

G

K

V

L

D

D

I

Y

E

E

R

L

V

K

Q

P

V

M

N

D

N
x
F

K

S

T

G

R

I

I

I

A

P

Y

A

V

L

Q

Q

Q

T

G

K

R

N

V

V

D

D

I

L

S

A

V

L

S
x
A

N
x
G

M

I

T
|
T

H

I

T
x
C

R
x
D

E
|
E

R
|
R

D

K

K

K

S

A

V

I

D

D

F

F

S

S

I

D

T

G

Y

Y

F

Y

F

K

D

S

G

G

Q

L

K

L

V

V

L

M

A

V

K

K

-

A

-

N

K

N

G

N

Q

D

F

V

K

K

S

S

V

V

K

K

D

D

M

L

V

D

G

G

K

K

K

V

I

V

A

A

T

V

M
x
K

Q

S

G

G

T
|
T

T
x
G

S

S

E

V

V
x
D

N

Y

V

A

K

K

R

A

A

N

L

I

K

K

E

T

A

-

G

-

D

-

P

-

N

-

P

-

D

-

Q

K

S

D

V

L

I

R

S

Q

F

F

Q

P

K

N

E

I

S

D

E

N

C

A

F

Y

Q

M

A

E

L

L

Q

G

M

T

G

N

R

R

V

A

A

D

G

A

W

V

S

L

T
x
H

D
|
D

A

T

-

P

S

N

I

I

L

L

V

Y

G

F

Y

I

S

K

A

T

K

A

E

G

P

N

G

G

K

Q

F

F

E

K

L

A

V

V

G

G

N

D

F

S

L

L

A
x
E

E

A

E

Q

P

Q

Y

Y

G

G

I

I

A

A

M

F

P

P

Q

K

D

G

D

S

S

D

A

E

L

L

R

R

D

D

A

K

V

V

N

N

A

G

A

A

L

L

Q

K

D

T

M

L

W

R

K

E

D

N

G

G

T

T

Y

Y

K

N

T

E

I

I

Y

Y

N

K

K

K

W

W

Y

F

G

G

binding glutamine: D10 (≠ M41), F13 (≠ S44), F50 (≠ N82), A67 (≠ S99), G68 (≠ N100), T70 (= T102), R75 (= R107), K115 (≠ M145), T118 (= T148), G119 (≠ T149), H156 (≠ T193), D157 (= D194)
binding azidobis[N~2~,N~3~-dihydroxybut-2-ene-2,3-diaminato(2-)-kappa~2~N,N']cobalt: C72 (≠ T104), D73 (≠ R105), E74 (= E106), G119 (≠ T149), D122 (≠ V152), E181 (≠ A217)

2ia4B Crystal structure of novel amino acid binding protein from shigella flexneri
26% identity, 89% coverage: 19:262/275 of query aligns to 1:251/278 of 2ia4B

query
sites
2ia4B

S

A

A

A

A

P

L

A

A

A

G

G

P

S

A

T

Y

L

D

D

N

K

V

I

M

A

K

K

D

N

K

G

K

V

I

I

R

V

V

V

G

G

L

H

M
x
R

T

E

D

S

S

S

I

V

P

P

G

F

G

S

F

Y

Y

Y

N

D

E

N

K

Q

G

Q

E

K

W

V

G

V

G

G

F

Y

D

S

Y

Q

D

D

-

Y

-

S

-

N

-

A

I

I

A

V

T

E

E

A

I

V

A

K

K

K

R

K

M

L

G

N

-

K

-

P

-

D

V

L

D

Q

I

V

E

K

R

L

V

I

Q

P

V

I

N

T

N
x
S

K

Q

T

N

R
|
R

I

I

A

P

Y

L

V

L

Q

Q

Q

N

G

G

R

T

V

F

D

D

I

F

S

E

V

C

S

G

N
x
S

M
x
T

T
|
T

H

N

T

N

R

V

E

E

R
|
R

D

Q

K

K

S

Q

V

A

D

A

F

F

S

S

I

D

T

T

Y

I

F

F

F

V

D

V

G

G

Q

T

K

R

V

L

L

L

A

T

K

K

-

G

G

G

Q

D

F

I

K

K

S

D

V

F

K

A

D

D

M

L

V

K

G

G

K

K

K

A

I

V

A

V

T

V

M

T

Q

S

G

G

T
|
T

S

S

E

E

V

V

N

L

V

L

K

N

R

K

A

L

L

N

K

E

E

E

A

Q

G

-

D

-

P

-

N

K

P

M

D

N

Q

M

S

R

V

I

I

I

S

S

F

A

Q

K

K

D

E
x
H

S

G

E

D

C

S

F

F

Q

R

A

T

L

L

Q

E

M

S

G

G

R

R

V

A

A

V

G

A

W

F

S

M

T
x
M

D
|
D

A

D

S

A

I
x
L

L

L

V

A

G

G

-

E

-

R

Y

A

S

K

A

A

K

K

E

K

P

P

G

D

K

N

F

W

E

D

L

I

V

V

G

G

N

K

F

P

L

Q

A

S

E

Q

E
|
E

P

A

Y
|
Y

G

G

I

C

A

M

M

L

P

R

Q

K

D

D

S

P

A

Q

L

F

R

K

D

K

A

L

V

M

N

D

A

D

A

T

L

I

Q

A

D

Q

M

V

W

Q

K

T

D

S

G

G

T

E

Y

A

K

E

T

K

I

W

Y

F

N

D

K

K

W

W

Y

F

G

K

P

N

G

P

T

I

P

P

binding glutamic acid: R23 (≠ M41), S71 (≠ N82), R74 (= R85), S89 (≠ N100), T90 (≠ M101), T91 (= T102), R96 (= R107), T138 (= T148), T139 (= T149), H163 (≠ E176), M180 (≠ T193), D181 (= D194), L184 (≠ I197), E208 (= E219), Y210 (= Y221)

2vhaA Debp (see paper)
26% identity, 88% coverage: 20:262/275 of query aligns to 1:250/276 of 2vhaA

query
sites
2vhaA

A

A

A

P

L

A

A

A

G

G

P

S

A

T

Y

L

D

D

N

K

V

I

M

A

K

K

D

N

K

G

K

V

I

I

R

V

V

V

G

G

L

H

M
x
R

T

E

D

S

S

S

I

V

P

P

G

F

G

S

F

Y

Y

Y

N

D

E

N

K

Q

G

Q

E

K

W

V

G

V

G

G

F

Y

D

S

Y

Q

D

D

-

Y

-

S

-

N

-

A

I

I

A

V

T

E

E

A

I

V

A

K

K

K

R

K

M

L

G

N

-

K

-

P

-

D

V

L

D

Q

I

V

E

K

R

L

V

I

Q

P

V

I

N

T

N
x
S

K

Q

T

N

R
|
R

I

I

A

P

Y

L

V

L

Q

Q

Q

N

G

G

R

T

V

F

D

D

I

F

S

E

V

C

S

G

N
x
S

M
x
T

T
|
T

H

N

T

N

R

V

E

E

R
|
R

D

Q

K

K

S

Q

V

A

D

A

F

F

S

S

I

D

T

T

Y

I

F

F

F

V

D

V

G

G

Q

T

K

R

V

L

L

L

A

T

K

K

-

G

G

G

Q

D

F

I

K

K

S

D

V

F

K

A

D

D

M

L

V

K

G

G

K

K

K

A

I

V

A

V

T

V

M

T

Q

S

G

G

T
|
T

S

S

E

E

V

V

N

L

V

L

K

N

R

K

A

L

L

N

K

E

E

E

A

Q

G

-

D

-

P

-

N

K

P

M

D

N

Q

M

S

R

V

I

I

I

S

S

F

A

Q

K

K

D

E
x
H

S

G

E

D

C

S

F

F

Q

R

A

T

L

L

Q

E

M

S

G

G

R

R

V

A

A

V

G

A

W

F

S

M

T
x
M

D
|
D

A

D

S

A

I
x
L

L

L

V

A

G

G

-

E

-

R

Y

A

S

K

A

A

K

K

E

K

P

P

G

D

K

N

F

W

E

D

L

I

V

V

G

G

N

K

F

P

L

Q

A

S

E

Q

E
|
E

P

A

Y

Y

G

G

I

C

A

M

M

L

P

R

Q

K

D

D

S

P

A

Q

L

F

R

K

D

K

A

L

V

M

N

D

A

D

A

T

L

I

Q

A

D

Q

M

V

W

Q

K

T

D

S

G

G

T

E

Y

A

K

E

T

K

I

W

Y

F

N

D

K

K

W

W

Y

F

G

K

P

N

G

P

T

I

P

P

binding glutamic acid: R22 (≠ M41), S70 (≠ N82), R73 (= R85), S88 (≠ N100), T89 (≠ M101), T90 (= T102), R95 (= R107), T137 (= T148), T138 (= T149), H162 (≠ E176), M179 (≠ T193), D180 (= D194), L183 (≠ I197), E207 (= E219)

5lomB Crystal structure of the pbp soca from agrobacterium tumefaciens c58 in complex with dfg at 1.5 a resolution (see paper)
29% identity, 85% coverage: 36:268/275 of query aligns to 14:239/250 of 5lomB

query
sites
5lomB

I

I

R

S

V

V

G

G

L

T

M
|
M

T

G

D

D

S

A

I

K

P

P

G

Y

G

A

F

F

Y

T

N

T

E

A

K

D

G

G

E

N

W

F

G

T

G

G

F

F

D

D

Y

I

D

E

I

L

A

F

T

L

E

N

I

V

A

A

K

G

R

R

M

L

G

G

V

F

D

K

I

K

E

E

R

Q

V

V

Q

V

V

F

N

T

N

G

K

Q

T

E

R
x
F

I

S

A

A

Y

L

-

M

-

P

-

S

V

V

Q

A

Q

N

G

G

R

R

V

F

D

D

I

V

S

A

V

A

S
x
A

N
x
A

M
x
I

T
x
G

H

T

T

T

R

A

E

K

R
|
R

D

K

K

E

S

T

V

V

D

D

F

F

S

S

I

D

T

G

Y

Y

F
x
L

F
x
A

D
x
G

G

F

Q

L

K

S

V

V

L

L

A

T

K

S

K

E

G

A

Q

G

F

I

K

T

S

D

V

A

K

A

D

G

M

L

V

K

G

G

K

K

K

R

I

L

A

G

T

V

M

V

Q

Q

G

G

T
|
T

T
x
L

S
x
Q

E

E

V

I

N

Y

V

A

K

E

R

K

A

N

L

F

K

-

E

-

A

A

G

G

D

-

P

-

N

-

P

-

D

-

Q

T

S

D

V

L

I

V

S

K

F

F

Q

P

K

D

E

N

S

N

E

S

C

A

F

V

Q

S

A

A

L

L

Q

N

M

N

G

G

R

T

V

V

A

D

G

A

W

H

S

F

T

L

D
|
D

A

F

S

E

I

A

L

A

V

K

G

D

Y

Y

S

S

A

A

K

R

E

Y

P

P

G

A

K

L

F

K

E

I

L

A

V

V

G

N

N

I

F

P

L
x
S

A

F

E

D

E
x
A

P

P

Y
x
A

G

G

I

F

A

V

M

I

P

R

Q

K

D

G

D

N

S

D

A

A

L

L

R

R

D

N

A

A

V

L

N

D

A

K

A

G

L

L

Q

K

D

E

M

A

W

M

K

Q

D

D

G

G

T

T

Y

W

K

K

T

K

I

L

Y

H

N

E

K

K

W

W

Y

F

G

-

P

P

G

G

T

T

P

P

F

-

E

-

M

M

P

P

L

A

A

A

binding Deoxyfructosylglutamine: M19 (= M41), F63 (≠ R85), A80 (≠ S99), A81 (≠ N100), I82 (≠ M101), G83 (≠ T102), R88 (= R107), L99 (≠ F118), A100 (≠ F119), G101 (≠ D120), T129 (= T148), L130 (≠ T149), Q131 (≠ S150), D168 (= D194), S190 (≠ L216), A193 (≠ E219), A195 (≠ Y221)

5l9oB Crystal structure of agrobacterium tumefaciens c58 strain pbp soca in complex with glucopine (see paper)
29% identity, 85% coverage: 36:268/275 of query aligns to 13:238/243 of 5l9oB

query
sites
5l9oB

I

I

R

S

V

V

G

G

L

T

M
|
M

T

G

D

D

S

A

I

K

P

P

G

Y

G

A

F

F

Y

T

N

T

E

A

K

D

G

G

E
x
N

W
x
F

G

T

G

G

F

F

D

D

Y

I

D

E

I

L

A

F

T

L

E

N

I

V

A

A

K

G

R

R

M

L

G

G

V

F

D

K

I

K

E

E

R

Q

V

V

Q

V

V

F

N

T

N

G

K

Q

T

E

R
x
F

I

S

A

A

Y

L

-

M

-

P

-

S

V

V

Q

A

Q

N

G

G

R

R

V

F

D

D

I

V

S

A

V

A

S
x
A

N
x
A

M
x
I

T
x
G

H

T

T

T

R

A

E

K

R
|
R

D

K

K

E

S

T

V

V

D

D

F

F

S

S

I

D

T

G

Y

Y

F
x
L

F
x
A

D
x
G

G
x
F

Q

L

K

S

V

V

L

L

A

T

K

S

K

E

G

A

Q

G

F

I

K

T

S

D

V

A

K

A

D

G

M

L

V

K

G

G

K

K

K

R

I

L

A

G

T

V

M

V

Q

Q

G

G

T
|
T

T
x
L

S
x
Q

E

E

V

I

N

Y

V

A

K

E

R

K

A

N

L

F

K

-

E

-

A

A

G

G

D

-

P

-

N

-

P

-

D

-

Q

T

S

D

V

L

I

V

S

K

F

F

Q

P

K

D

E

N

S

N

E

S

C

A

F

V

Q

S

A

A

L

L

Q

N

M

N

G

G

R

T

V

V

A

D

G

A

W

H

S

F

T

L

D
|
D

A

F

S

E

I

A

L

A

V

K

G

D

Y

Y

S

S

A

A

K

R

E

Y

P

P

G

A

K

L

F

K

E

I

L

A

V

V

G

N

N

I

F

P

L
x
S

A

F

E

D

E
x
A

P

P

Y
x
A

G

G

I

F

A

V

M

I

P

R

Q

K

D

G

D

N

S

D

A

A

L

L

R

R

D

N

A

A

V

L

N

D

A

K

A

G

L

L

Q

K

D

E

M

A

W

M

K

Q

D

D

G

G

T

T

Y

W

K

K

T

K

I

L

Y

H

N

E

K

K

W

W

Y

F

G

-

P

P

G

G

T

T

P

P

F

-

E

-

M

M

P

P

L

A

A

A

binding calcium ion: N32 (≠ E55), F33 (≠ W56)
binding Glucopine: M18 (= M41), F62 (≠ R85), A79 (≠ S99), A80 (≠ N100), I81 (≠ M101), G82 (≠ T102), R87 (= R107), L98 (≠ F118), A99 (≠ F119), G100 (≠ D120), F101 (≠ G121), T128 (= T148), L129 (≠ T149), Q130 (≠ S150), D167 (= D194), S189 (≠ L216), A192 (≠ E219), A194 (≠ Y221)

5l9oA Crystal structure of agrobacterium tumefaciens c58 strain pbp soca in complex with glucopine (see paper)
29% identity, 85% coverage: 36:268/275 of query aligns to 12:237/241 of 5l9oA

query
sites
5l9oA

I

I

R

S

V

V

G

G

L

T

M
|
M

T

G

D

D

S

A

I

K

P

P

G

Y

G

A

F

F

Y

T

N

T

E

A

K

D

G

G

E

N

W

F

G

T

G

G

F

F

D

D

Y

I

D

E

I

L

A

F

T

L

E

N

I

V

A

A

K

G

R

R

M

L

G

G

V

F

D

K

I

K

E

E

R

Q

V

V

Q

V

V

F

N

T

N

G

K

Q

T

E

R
x
F

I

S

A

A

Y

L

-

M

-

P

-

S

V

V

Q

A

Q

N

G

G

R

R

V

F

D

D

I

V

S

A

V

A

S
x
A

N
x
A

M
x
I

T
x
G

H

T

T

T

R

A

E

K

R
|
R

D

K

K

E

S

T

V

V

D

D

F

F

S

S

I

D

T

G

Y

Y

F
x
L

F
x
A

D
x
G

G
x
F

Q
x
L

K

S

V

V

L

L

A

T

K

S

K

E

G

A

Q

G

F

I

K

T

S

D

V

A

K

A

D

G

M

L

V

K

G

G

K

K

K

R

I

L

A

G

T

V

M

V

Q

Q

G

G

T
|
T

T
x
L

S
x
Q

E

E

V

I

N

Y

V

A

K

E

R

K

A

N

L

F

K

-

E

-

A

A

G

G

D

-

P

-

N

-

P

-

D

-