SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 206320 MicrobesOnline__882:206320 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P10880 Shikimate kinase 2; SK 2; Shikimate kinase II; SKII; EC 2.7.1.71 from Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi) (see paper)
47% identity, 90% coverage: 8:165/175 of query aligns to 5:161/173 of P10880

query
sites
P10880

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C13 (≠ S16) mutation to S: 66% of wild-type activity. Increase in substrates affinity.
K15 (= K18) mutation to M: Loss of activity. Increased thermostability.
D34 (= D37) mutation to N: Loss of activity.

Sites not aligning to the query:

162 C→S: No effect on activity and substrates affinity.

1e6cA K15m mutant of shikimate kinase from erwinia chrysanthemi (see paper)
46% identity, 90% coverage: 8:165/175 of query aligns to 5:161/170 of 1e6cA

query
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1e6cA

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binding phosphate ion: R11 (= R14), G12 (≠ A15), M15 (≠ K18), T16 (≠ S19)

2shkB The three-dimensional structure of shikimate kinase from erwinia chrysanthemi complexed with adp (see paper)
44% identity, 90% coverage: 8:165/175 of query aligns to 5:151/162 of 2shkB

query
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2shkB

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binding adenosine-5'-diphosphate: G12 (≠ A15), C13 (≠ S16), G14 (= G17), K15 (= K18), T16 (≠ S19), T17 (= T20), R110 (= R114), Q145 (≠ A159), P147 (≠ V161)
binding magnesium ion: Q134 (≠ A148), A137 (= A151)

1shkA The three-dimensional structure of shikimate kinase from erwinia chrysanthemi (see paper)
42% identity, 90% coverage: 8:165/175 of query aligns to 5:146/158 of 1shkA

query
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1shkA

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binding magnesium ion: R92 (= R96), A93 (≠ E97), G95 (= G99), Q129 (≠ A148), A132 (= A151)

4y0aA Shikimate kinase from acinetobacter baumannii in complex with shikimate (see paper)
34% identity, 88% coverage: 5:158/175 of query aligns to 8:158/179 of 4y0aA

query
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4y0aA

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binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: M17 (≠ R14), D40 (= D37), F63 (= F60), R64 (= R61), G86 (= G83), G87 (= G84), L126 (≠ S126), R143 (= R143)
binding sulfate ion: G18 (≠ A15), A19 (≠ S16), G20 (= G17), K21 (= K18), T22 (≠ S19), E112 (= E109), R124 (= R124), R137 (≠ A137)

1u8aA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and adp at 2.15 angstrom resolution (see paper)
40% identity, 81% coverage: 7:147/175 of query aligns to 3:137/163 of 1u8aA

query
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1u8aA

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binding adenosine-5'-diphosphate: G11 (≠ A15), G13 (= G17), K14 (= K18), S15 (= S19), T16 (= T20), R109 (≠ N118)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D37), F48 (≠ V52), R57 (= R61), G79 (= G83), G80 (= G84), L116 (≠ S126), R133 (= R143)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 150, 152

2iyvA Shikimate kinase from mycobacterium tuberculosis in complex with adp, open lid (conf. B) (see paper)
39% identity, 81% coverage: 7:147/175 of query aligns to 3:139/179 of 2iyvA

query
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2iyvA

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binding adenosine-5'-diphosphate: G11 (≠ A15), G13 (= G17), K14 (= K18), S15 (= S19), T16 (= T20), R109 (= R117)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 152, 154

2iywA Shikimate kinase from mycobacterium tuberculosis in complex with mgatp, open lid (conf. B) (see paper)
39% identity, 81% coverage: 7:147/175 of query aligns to 3:139/178 of 2iywA

query
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2iywA

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D

A

V

L

V

R

R

D

A

A

A

A

L

A

A

R

D

E

H

R

D

V

G

V

V

V

L

A

S

T

L

G

G

M

A

V

V

L

T

R

S

A

P

E

G

N

V

R

R

A

A

L

A

L

L

R

-

E

-

A

A

G

G

V

H

T

T

V

V

F

V

L

Y

S

-

L

-

P

-

V

L

E

E

V

I

V

S

A

A

R

E

-

G

L

V

A

R

R
|
R

N

T

P

G

E

G

H

N

G

T

Q

V

R

R

P

P

S

L

L

L

T

A

G

G

K

P

A

D

V

R

A

A

D

E

E

K

V

Y

A

R

D

A

V

L

M

M

R

A

E

K

R

R

A

A

P

P

L

L

Y

Y

binding adenosine-5'-triphosphate: G11 (≠ A15), G13 (= G17), K14 (= K18), S15 (= S19), T16 (= T20), R109 (= R117)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 152, 154

4bqsA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with adp and a shikimic acid derivative. (see paper)
39% identity, 81% coverage: 7:147/175 of query aligns to 3:139/169 of 4bqsA

query
sites
4bqsA

R

K

V

A

F

V

L

L

I

V

G

G

G

L

R
x
P

A
x
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

V

I

G

G

R

R

A

R

L

L

A

A

A

K

R

A

L

L

G

G

W

V

D

G

F

L

A

L

D

D

T

T

D
|
D

A

V

M

A

V

I

T

E

R

Q

R

R

A

T

G

G

C

R

D

S

I

I

A

A

T

D

L

I

V

F

A

A

E

T

R

D

G

G

W

E

D

Q

A

E

F

F

R

R

D

R

M

I

E

E

S

E

D

D

A

V

L

V

R

R

D

A

A

A

A

L

A

A

R

D

E

H

R

D

V

G

V

V

V

L

A

S

T

L

G

G

M

A

V

V

L

T

R

S

A

P

E

G

N

V

R

R

A

A

L

A

L

L

R

-

E

-

A

A

G

G

V

H

T

T

V

V

F

V

L

Y

S

-

L

-

P

-

V

L

E

E

V

I

V

S

A

A

R

E

-

G

L

V

A

R

R
|
R

N

T

P

G

E

G

H

N

G

T

Q

V

R

R

P

P

S

L

L

L

T

A

G

G

K

P

A

D

V

R

A

A

D

E

E

K

V

Y

A

R

D

A

V

L

M

M

R

A

E

K

R
|
R

A

A

P

P

L

L

Y

Y

binding adenosine-5'-diphosphate: P10 (≠ R14), G11 (≠ A15), G13 (= G17), K14 (= K18), S15 (= S19), T16 (= T20), R109 (= R117)
binding (1R,6R,10S)-6,10-dihydroxy-2-oxabicyclo[4.3.1]deca-4(Z),7-diene-8-carboxylic acid: D33 (= D37), R135 (= R143)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 152, 154

2iyyA Shikimate kinase from mycobacterium tuberculosis in complex with shikimate-3-phosphate and so4 (see paper)
39% identity, 81% coverage: 7:147/175 of query aligns to 3:139/169 of 2iyyA

query
sites
2iyyA

R

K

V

A

F

V

L

L

I

V

G

G

G

L

R

P

A

G

S

S

G

G

K
|
K

S

S

T

T

V

I

G

G

R

R

A

R

L

L

A

A

A

K

R

A

L

L

G

G

W

V

D

G

F

L

A

L

D

D

T

T

D
|
D

A

V

M

A

V

I

T
x
E

R

Q

R

R

A

T

G

G

C

R

D
x
S

I
|
I

A

A

T

D

L

I

V

F

A

A

E

T

R

D

G

G

W

E

D

Q

A

E

F

F

R
|
R

D

R

M

I

E

E

S

E

D

D

A

V

L

V

R

R

D

A

A

A

A

L

A

A

R

D

E

H

R

D

V

G

V

V

V

L

A

S

T

L

G

G

G
|
G

M

A

V

V

L

T

R

S

A

P

E

G

N

V

R

R

A

A

L

A

L

L

R

-

E

-

A

A

G

G

V

H

T

T

V

V

F

V

L

Y

S

-

L

-

P

-

V

L

E

E

V

I

V

S

A

A

R

E

-

G

L

V

A

R

R

R

N

T

P

G

E

G

H

N

G

T

Q

V

R

R

P

P

S
x
L

L

L

T

A

G

G

K

P

A

D

V

R

A

A

D

E

E

K

V

Y

A

R

D

A

V

L

M

M

R

A

E

K

R
|
R

A

A

P

P

L

L

Y

Y

binding magnesium ion: E37 (≠ T41), S43 (≠ D47), I44 (= I48)
binding phosphate ion: K14 (= K18), G79 (= G83)
binding shikimate-3-phosphate: K14 (= K18), D33 (= D37), R57 (= R61), G79 (= G83), G80 (= G84), L118 (≠ S126), R135 (= R143)

1zyuA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and amppcp at 2.85 angstrom resolution (see paper)
39% identity, 81% coverage: 7:147/175 of query aligns to 3:139/168 of 1zyuA

query
sites
1zyuA

R

K

V

A

F

V

L

L

I

V

G

G

G

L

R
x
P

A
x
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

V

I

G

G

R

R

A

R

L

L

A

A

A

K

R

A

L

L

G

G

W

V

D

G

F

L

A

L

D

D

T

T

D
|
D

A

V

M

A

V

I

T

E

R

Q

R

R

A

T

G

G

C

R

D

S

I

I

A

A

T

D

L

I

V

F

A

A

E

T

R

D

G

G

W

E

D

Q

A

E

F

F

R
|
R

D

R

M

I

E

E

S

E

D

D

A

V

L

V

R

R

D

A

A

A

A

L

A

A

R

D

E

H

R

D

V

G

V

V

V

L

A

S

T

L

G

G

G
|
G

M

A

V

V

L

T

R

S

A

P

E

G

N

V

R

R

A

A

L

A

L

L

R

-

E

-

A

A

G

G

V

H

T

T

V

V

F

V

L

Y

S

-

L

-

P

-

V

L

E

E

V

I

V

S

A

A

R

E

-

G

L

V

A

R

R
|
R

N

T

P

G

E

G

H

N

G

T

Q

V

R

R

P
|
P

S
x
L

L

L

T

A

G

G

K

P

A

D

V

R

A

A

D

E

E

K

V

Y

A

R

D

A

V

L

M

M

R

A

E

K

R
|
R

A

A

P

P

L

L

Y

Y

binding phosphomethylphosphonic acid adenylate ester: P10 (≠ R14), G11 (≠ A15), G13 (= G17), K14 (= K18), S15 (= S19), T16 (= T20), R109 (= R117)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D37), R57 (= R61), G80 (= G84), P117 (= P125), L118 (≠ S126), R135 (= R143)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenylate ester: 149, 152, 154

3bafA Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with amp-pnp
39% identity, 81% coverage: 7:147/175 of query aligns to 3:139/165 of 3bafA

query
sites
3bafA

R

K

V

A

F

V

L

L

I

V

G

G

G

L

R
x
P

A

G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

V

I

G

G

R

R

A

R

L

L

A

A

A

K

R

A

L

L

G

G

W

V

D

G

F

L

A

L

D

D

T

T

D
|
D

A

V

M

A

V

I

T

E

R

Q

R

R

A

T

G

G

C

R

D

S

I

I

A

A

T

D

L

I

V

F

A

A

E

T

R

D

G

G

W

E

D

Q

A

E

F

F

R

R

D

R

M

I

E

E

S

E

D

D

A

V

L

V

R

R

D

A

A

A

A

L

A

A

R

D

E

H

R

D

V

G

V

V

V

L

A

S

T

L

G

G

M

A

V

V

L

T

R

S

A

P

E

G

N

V

R

R

A

A

L

A

L

L

R

-

E

-

A

A

G

G

V

H

T

T

V

V

F

V

L

Y

S

-

L

-

P

-

V

L

E

E

V

I

V

S

A

A

R

E

-

G

L

V

A

R

R
|
R

N

T

P

G

E

G

H

N

G

T

Q

V

R
|
R

P

P

S

L

L

L

T

A

G

G

K

P

A

D

V

R

A

A

D

E

E

K

V

Y

A

R

D

A

V

L

M

M

R

A

E

K

R
|
R

A

A

P

P

L

L

Y

Y

binding phosphoaminophosphonic acid-adenylate ester: P10 (≠ R14), G13 (= G17), K14 (= K18), S15 (= S19), T16 (= T20), R109 (= R117), R116 (= R124)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D37), R135 (= R143)

2dfnA Structure of shikimate kinase from mycobacterium tuberculosis complexed with adp and shikimate at 1.9 angstrons of resolution (see paper)
39% identity, 81% coverage: 7:147/175 of query aligns to 3:139/165 of 2dfnA

query
sites
2dfnA

R

K

V

A

F

V

L

L

I

V

G

G

G

L

R
x
P

A
x
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

V

I

G

G

R

R

A

R

L

L

A

A

A

K

R

A

L

L

G

G

W

V

D

G

F

L

A

L

D

D

T

T

D
|
D

A

V

M

A

V

I

T

E

R

Q

R

R

A

T

G

G

C

R

D

S

I
|
I

A

A

T

D

L

I

V

F

A

A

E

T

R

D

G

G

W

E

D

Q

A

E

F
|
F

R
|
R

D

R

M

I

E

E

S

E

D

D

A

V

L

V

R

R

D

A

A

A

A

L

A

A

R

D

E

H

R

D

V

G

V

V

V

L

A

S

T

L

G
|
G

M

A

V

V

L

T

R

S

A

P

E

G

N

V

R

R

A

A

L

A

L

L

R

-

E

-

A

A

G

G

V

H

T

T

V

V

F

V

L

Y

S

-

L

-

P

-

V

L

E

E

V

I

V

S

A

A

R

E

-

G

L

V

A

R

R
|
R

N

T

P

G

E

G

H

N

G

T

Q

V

R
|
R

P

P

S

L

L

L

T

A

G

G

K

P

A

D

V

R

A

A

D

E

E

K

V

Y

A

R

D

A

V

L

M

M

R

A

E

K

R
|
R

A

A

P

P

L

L

Y

Y

binding adenosine-5'-diphosphate: G11 (≠ A15), S12 (= S16), G13 (= G17), K14 (= K18), S15 (= S19), T16 (= T20), R109 (= R117), R116 (= R124)
binding chloride ion: P10 (≠ R14), K14 (= K18), G79 (= G83)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D37), I44 (= I48), F56 (= F60), R57 (= R61), G78 (= G82), G79 (= G83), G80 (= G84), R135 (= R143)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 152, 154

1we2A Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with mgadp and shikimic acid (see paper)
39% identity, 81% coverage: 7:147/175 of query aligns to 3:139/165 of 1we2A

query
sites
1we2A

R

K

V

A

F

V

L

L

I

V

G

G

G

L

R
x
P

A
x
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

V

I

G

G

R

R

A

R

L

L

A

A

A

K

R

A

L

L

G

G

W

V

D

G

F

L

A

L

D

D

T

T

D
|
D

A

V

M

A

V

I

T

E

R

Q

R

R

A

T

G

G

C

R

D

S

I
|
I

A

A

T

D

L

I

V
x
F

A

A

E

T

R

D

G

G

W

E

D

Q

A

E

F

F

R

R

D

R

M

I

E

E

S

E

D

D

A

V

L

V

R

R

D

A

A

A

A

L

A

A

R

D

E

H

R

D

V

G

V

V

V

L

A

S

T

L

G
|
G

M

A

V

V

L

T

R

S

A

P

E

G

N

V

R

R

A

A

L

A

L

L

R

-

E

-

A

A

G

G

V

H

T

T

V

V

F

V

L

Y

S

-

L

-

P

-

V

L

E

E

V

I

V

S

A

A

R

E

-

G

L

V

A

R

R
|
R

N

T

P

G

E

G

H

N

G

T

Q

V

R
|
R

P

P

S
x
L

L

L

T

A

G

G

K

P

A

D

V

R

A

A

D

E

E

K

V

Y

A

R

D

A

V

L

M

M

R

A

E

K

R
|
R

A

A

P

P

L

L

Y

Y

binding adenosine-5'-diphosphate: P10 (≠ R14), G11 (≠ A15), G13 (= G17), K14 (= K18), S15 (= S19), T16 (= T20), R109 (= R117), R116 (= R124)
binding 3-dehydroshikimate: D33 (= D37), I44 (= I48), F48 (≠ V52), G78 (= G82), G79 (= G83), G80 (= G84), L118 (≠ S126), R135 (= R143)

3n2eA Crystal structure of helicobactor pylori shikimate kinase in complex with nsc162535 (see paper)
31% identity, 83% coverage: 10:155/175 of query aligns to 6:144/168 of 3n2eA

query
sites
3n2eA

L

L

I

I

G

G

G
x
F

R

M

A

G

S

S

G

G

K

K

S

S

T

S

V

L

G

A

R

Q

A

E

L

L

A

G

A

L

R

A

L

L

G

K

W

L

D

E

F

V

A

L

D

D

T

T

D

D

A

M

M

I

V

I

T

S

R

E

R

R

A

V

G

G

C

L

D

S

I

V

A
x
R

T

E

L

I

V
x
F

A

E

E

E

R

L

G

G

W

E

D

D

A

N

F

F

R
|
R

D

M

M

F

E

E

S

K

D

N

A

L

L

I

R

D

D

E

-

L

A

K

A

T

A

L

R

K

E

T

R

P

V

H

V

V

V

I

A

S

T

T

G

G

G
|
G

M

I

V

V

L
x
M

R

H

A

-

E

E

N

N

R

-

A

-

L

-

L

L

R

K

E

G

A

L

G

G

V

T

T

T

V

F

F

Y

L

L

S

K

L

M

P

D

V

F

E

E

V

T

V

L

A

I

A

K

R

R

L

L

A

N

R

Q

N

-

P

K

E

E

H

R

G

A

Q

K

R
|
R

P

P

S

L

L

L

T

N

G

N

K

L

A

T

V

Q

A

A

D

K

E

E

V

L

A

F

D

E

V

-

M

-

R

-

E

K

R
|
R

A

Q

P

A

L
|
L

Y
|
Y

A

E

E

K

A

N

A

A

R

S

H

F

V

I

binding 7-amino-4-hydroxy-3-[(E)-(5-hydroxy-7-sulfonaphthalen-2-yl)diazenyl]naphthalene-2-sulfonic acid: F9 (≠ G13), R45 (≠ A49), F48 (≠ V52), R57 (= R61), G80 (= G83), G81 (= G84), M84 (≠ L87), R116 (= R124), R132 (= R143), L135 (= L146), Y136 (= Y147)

3mufA Shikimate kinase from helicobacter pylori in complex with shikimate-3- phosphate and adp (see paper)
31% identity, 83% coverage: 10:155/175 of query aligns to 5:143/160 of 3mufA

query
sites
3mufA

L

L

I

I

G

G

G

F

R
x
M

A
x
G

S
|
S

G
|
G

K
|
K

S
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S

T
x
S

V

L

G

A

R

Q

A

E

L

L

A

G

A

L

R

A

L

L

G

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W

L

D

E

F

V

A

L

D

D

T

T

D
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D

A

M

M

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T

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E

R

R

A

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G

C

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T

E

L

I

V

F

A

E

E

E

R

L

G

G

W

E

D

D

A

N

F

F

R
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R

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M

M

F

E

E

S

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D

N

A

L

L

I

R

D

D

E

-

L

A

K

A

T

A

L

R

K

E

T

R

P

V

H

V

V

V

I

A

S

T

T

G
|
G

M

I

V

V

L

M

R

H

A

-

E

E

N

N

R

-

A

-

L

-

L

L

R

K

E

G

A

L

G

G

V

T

T

T

V

F

F

Y

L

L

S

K

L
x
M

P

D

V

F

E

E

V

T

V

L

A

I

A

K

R
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R

L

L

A

N

R

Q

N

-

P

K

E

E

H

R

G

E

Q

K

R
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R

P

P

S

L

L

L

T

N

G

N

K

L

A

T

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Q

A

A

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K

E

E

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L

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F

D

E

V

-

M

-

R

-

E

K

R
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R

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Q

P

A

L

L

Y

Y

A

E

E

K

A

N

A

A

R

S

H

F

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I

binding adenosine-5'-diphosphate: G10 (≠ A15), S11 (= S16), G12 (= G17), K13 (= K18), S14 (= S19), S15 (≠ T20), M98 (≠ L106), R106 (= R114)
binding shikimate-3-phosphate: M9 (≠ R14), D32 (= D37), R56 (= R61), G78 (= G82), G79 (= G83), G80 (= G84), R115 (= R124), R131 (= R143)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 147, 148

1zuiA Structural basis for shikimate-binding specificity of helicobacter pylori shikimate kinase (see paper)
30% identity, 83% coverage: 10:155/175 of query aligns to 5:141/158 of 1zuiA

query
sites
1zuiA

L

L

I

I

G

G

G

F

R
x
M

A

G

S

S

G

G

K

K

S

S

T

S

V

L

G

A

R

Q

A

E

L

L

A

G

A

L

R

A

L

L

G

K

W

L

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D

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M

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R

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V

G

G

C

L

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x
F

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E

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L

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G

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R

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N

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T

G
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G

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G

M

I

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L

M

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A

-

E

E

N

N

R

-

A

-

L

-

L

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G

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x
E

E

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R

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P

S

L

L

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R

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A

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L

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Y

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K

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N

A

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I

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: M9 (≠ R14), D32 (= D37), F47 (≠ V52), R56 (= R61), G78 (= G82), G79 (= G83), E111 (≠ P119), R113 (= R124), R129 (= R143)

6hqvA Pentafunctional arom complex from chaetomium thermophilum (see paper)
29% identity, 83% coverage: 8:152/175 of query aligns to 845:988/1555 of 6hqvA

query
sites
6hqvA

V

I

F

F

L

I

I

V

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G

G

M

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D

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G

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F

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A

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L

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L

L

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D

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A

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N

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Q

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K

-

G

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Y

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G

G
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G

M

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R

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E

E

A

N

A

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R

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K

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L

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I

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-

Y

-

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V

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V

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D

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V

V

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V

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E

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Y

L

L

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M

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R

N

D

P

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E

-

H

-

G

K

Q

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S

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L

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T

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G

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K

-

A

A

V

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S

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E

N

V

I

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R

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E

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Y

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E

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R
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R

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K

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P

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Y

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A

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R

N

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D874 (= D37), G923 (= G83), G924 (= G84), R979 (= R143)

Sites not aligning to the query:

active site: 123, 145, 187, 243, 253, 257, 261, 264, 268, 280
binding (4S,5R)-4,5-dihydroxy-3-oxocyclohex-1-ene-1-carboxylic acid: 1060, 1062, 1181, 1224, 1232, 1242, 1243
binding glutamic acid: 139, 145, 187, 243, 257, 264, 280
binding nicotinamide-adenine-dinucleotide: 42, 44, 45, 76, 79, 107, 108, 109, 112, 132, 133, 135, 139, 140, 145, 154, 175, 176, 177, 180, 280
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 413, 562, 563, 1277, 1279, 1323, 1327, 1348, 1368, 1526
binding zinc ion: 187, 264, 280

P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
36% identity, 54% coverage: 8:101/175 of query aligns to 866:961/1583 of P07547

query
sites
P07547

V

I

F

Y

L

I

I

I

G

G

G

M

R

R

A

G

S

A

G

G

K

K

S

S

T

T

V

A

G

G

R

N

A

W

L

V

A

S

A

K

R

A

L

L

G

N

W

R

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F

F

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D

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D

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L

L

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K

R

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T

A

L

A

K

A

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-

G

V

Y

V

V

V

F

A

A

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A

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L

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Y

G

H

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K

T

T

Sites not aligning to the query:

44:46 binding
81:84 binding
114:116 binding
119 binding
139:140 binding
161 binding
179:182 binding
190 binding
194 binding
271 binding
287 binding

7tbvA Crystal structure of the shikimate kinase + 3-dehydroquinate dehydratase + 3-dehydroshikimate dehydrogenase domains of aro1 from candida albicans (see paper)
32% identity, 51% coverage: 8:96/175 of query aligns to 6:96/682 of 7tbvA

query
sites
7tbvA

V

I

F

I

L

V

I

I

G

G

G

M

R

R

A

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F

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A

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D

E

A

C

A

F

A

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F

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S

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Y

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V

V

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T

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G

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V

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R

G

A

E

E

D

N

A

R

R

A

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L

Q

L

L

R

K

Sites not aligning to the query:

binding magnesium ion: 300, 302, 329

Query Sequence

>206320 MicrobesOnline__882:206320
MGSEVCRVFLIGGRASGKSTVGRALAARLGWDFADTDAMVTRRAGCDIATLVAERGWDAF
RDMESDALRDAAARERVVVATGGGMVLRAENRALLREAGVTVFLSLPVEVVAARLARNPE
HGQRPSLTGKAVADEVADVMRERAPLYAEAARHVVRGDAPVACIVEDITALLAFS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory