SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 207007 MicrobesOnline__882:207007 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1dgjA Crystal structure of the aldehyde oxidoreductase from desulfovibrio desulfuricans atcc 27774 (see paper)
69% identity, 100% coverage: 1:903/906 of query aligns to 1:905/906 of 1dgjA

query
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1dgjA

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active site: V391 (≠ I390), F427 (= F425), R503 (= R501), Y507 (≠ F505), R535 (= R533), E869 (= E867), M870 (≠ L868)
binding molybdenum (iv)oxide: G424 (= G422), R535 (= R533), G698 (= G696), E869 (= E867)
binding fe2/s2 (inorganic) cluster: V38 (= V38), G39 (= G39), C40 (= C40), G41 (= G41), G43 (= G43), Q44 (≠ H44), C45 (= C45), G46 (= G46), C48 (= C48), R58 (= R58), C60 (= C60), C100 (= C100), G101 (= G101), C103 (= C103), C137 (= C137), C139 (= C139)
binding pterin cytosine dinucleotide: Q99 (= Q99), C139 (= C139), F423 (= F421), G424 (= G422), R535 (= R533), W652 (= W650), H655 (= H653), G656 (= G654), Q657 (= Q655), G658 (= G656), A697 (= A695), G698 (= G696), S700 (= S698), S702 (= S700), Q703 (= Q701), C799 (≠ V797), N800 (= N798), V803 (≠ T801), V804 (= V802), Q807 (= Q805), S865 (≠ G863), G866 (= G864), V867 (= V865), G868 (= G866), E869 (= E867)

4usaA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with trans-cinnamaldehyde (see paper)
70% identity, 100% coverage: 1:904/906 of query aligns to 1:906/907 of 4usaA

query
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4usaA

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active site: I390 (= I390), F425 (= F425), R501 (= R501), F505 (= F505), R533 (= R533), E869 (= E867), L870 (= L868)
binding bicarbonate ion: R460 (= R460), A531 (= A531), F532 (= F532), Y535 (= Y535), Q539 (= Q539)
binding fe2/s2 (inorganic) cluster: V38 (= V38), C40 (= C40), E41 (≠ G41), G43 (= G43), C45 (= C45), G46 (= G46), C48 (= C48), R58 (= R58), C60 (= C60), C100 (= C100), G101 (= G101), C103 (= C103), C137 (= C137), C139 (= C139)
binding hydrocinnamic acid: I255 (= I255), F425 (= F425), F494 (= F494), L497 (= L497), Y535 (= Y535), L626 (= L626)
binding magnesium ion: E899 (= E897), E903 (≠ A901)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q99), C139 (= C139), T420 (= T420), F421 (= F421), G422 (= G422), R533 (= R533), W650 (= W650), H653 (= H653), G654 (= G654), Q655 (= Q655), G656 (= G656), S695 (≠ A695), G696 (= G696), G697 (= G697), Q700 (≠ S700), Q701 (= Q701), C799 (≠ V797), N800 (= N798), T804 (≠ V802), Q807 (= Q805), S865 (≠ G863), G866 (= G864), V867 (= V865), G868 (= G866), E869 (= E867)

4us9A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with 3- phenylpropionaldehyde (see paper)
70% identity, 100% coverage: 1:904/906 of query aligns to 1:906/907 of 4us9A

query
sites
4us9A

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I

K

R

A

V

G

A

C

C

E

E

L

N

L

L

G

K

G

A

M

C

R

E

K

K

A

P

D

G

G

G

T

G

F

Y

R

Y

T

T

Y

Y

A

D

E

E

M

L

R

K

D

A

E

A

K

D

I

K

A

P

T

T

R

K

Y

I

S

T

G

G

K

N

W

W

S

T

A

A

P

S

-

G

C

A

T

T

D
x
H

C

C

N

D

A

A

-

V

E

T

G

G

Q

L

G

G

S

K

P

P

F

F

A

V

V

V

Y

Y

M

M

Y

Y

G

G

V

V

F

F

L

M

A

A

E

E

V

V

S

T

V

V

E

D

L

V

A

A

T

T

G

G

K

Q

T

T

V

V

D

D

K

G

M

M

T

T

L

L

V

M

A

A

D

D

I

L

G

G

K

S

I

L

V
x
C

N
|
N

R

Q

L

L

T

A

V
x
T

D

D

G

G

Q
|
Q

L

I

Y

Y

G

G

I

L

A

A

Q

Q

G

G

I

I

G

G

L

L

A

A

L

L

S

S

E

E

D

D

F

F

E

E

D

D

I

I

K

K

H

H

S

A

T

T

M

L

P

V

G

G

A

A

G

G

F

F

P

P

Y

F

I

I

K

K

Q

Q

I

I

P

P

D

D

E

K

I

L

D

D

L

I

V

V

Y

Y

V

V

E

N

V

H

P

P

R

R

P

P

E

D

G

G

P

P

F

F

G

G

A

A

G
x
S

G
|
G

V
|
V

G
|
G

E
|
E

L
|
L

P

P

L

L

T

T

C

S

P

P

H

H

A

A

I

I

N

N

A

A

I

I

H

K

N

S

A

A

C

T

G

G

V

V

R
|
R

V

I

T
x
Y

R

R

L

L

P

P

A

A

L

Y

P

P

E
|
E

K

K

V

V

L

L

A
x
E

G

A

L

L

Q

K

active site: I390 (= I390), F425 (= F425), R501 (= R501), F505 (= F505), R533 (= R533), E869 (= E867), L870 (= L868)
binding 3-phenylpropanal: I255 (= I255), F257 (= F257), P258 (= P258), H752 (≠ D751)
binding bicarbonate ion: R460 (= R460), L498 (= L498), A531 (= A531), F532 (= F532), Y535 (= Y535), Q539 (= Q539), R890 (= R888), Y892 (≠ T890)
binding fe2/s2 (inorganic) cluster: V38 (= V38), C40 (= C40), E41 (≠ G41), G43 (= G43), C45 (= C45), G46 (= G46), C48 (= C48), R58 (= R58), C60 (= C60), C100 (= C100), G101 (= G101), C103 (= C103), C137 (= C137), C139 (= C139)
binding magnesium ion: E899 (= E897), E903 (≠ A901)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q99), C139 (= C139), T420 (= T420), F421 (= F421), G422 (= G422), R533 (= R533), W650 (= W650), H653 (= H653), G654 (= G654), Q655 (= Q655), G656 (= G656), S695 (≠ A695), G696 (= G696), G697 (= G697), Q700 (≠ S700), Q701 (= Q701), C799 (≠ V797), N800 (= N798), T804 (≠ V802), Q807 (= Q805), S865 (≠ G863), G866 (= G864), V867 (= V865), G868 (= G866), E869 (= E867)

4us8A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with benzaldehyde (see paper)
70% identity, 100% coverage: 1:904/906 of query aligns to 1:906/907 of 4us8A

query
sites
4us8A

M

M

I

I

K

Q

R

K

M

V

V

I

T

T

I

V

N

N

G

G

A

I

P

E

R

Q

M

N

A

L

I

F

T

V

R

D

P

A

D

E

T

A

T

L

L

L

A

S

T

D

Y

V

L

L

R

R

E

Q

S

Q

L

L

G

G

L

L

T

T

S

G

V

V

K

K

V
|
V

G

G

C
|
C

G
x
E

Q

Q

G
|
G

H

Q

C
|
C

G
|
G

S

A

C
|
C

N

S

V

V

I

I

V

L

D

D

G

G

K

K

L

V

V

V

R
|
R

S

A

C
|
C

S

V

Y

T

K

K

M

M

S

K

R

R

L

V

T

A

E

D

G

G

A

A

T

Q

I

I

T

T

L

I

E

E

G

G

I

V

G

G

T

Q

P

P

D

E

N

N

L

L

H

H

P

P

L

L

Q

Q

V

K

A

A

W

W

M

V

A

L

H

H

G

G

A

G

A

A

Q
|
Q

C
|
C

G
|
G

F

F

C
|
C

S

S

P

P

G

G

F

F

I

I

V

V

S

S

A

A

K

K

A

G

L

L

I

L

D

D

T

T

N

N

P

A

K

D

P

P

S

S

R

R

D

E

D

D

V

V

R

R

D

D

W

W

F

F

Q

Q

K

K

H

H

R

R

N

N

A

A

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

K

K

P

P

L

L

V

V

D

D

A

A

V

V

M

M

D

D

A

A

V

V

V

I

R

N

G

G

E

K

M

K

K

P

V

E

D

T

D

D

L

L

L

E

Y

F

R

K

I

M

P

P

A

A

D

D

G

G

R

R

I

I

W

W

G

G

T

S

K

K

Y

Y

P

P

R

R

P

P

S

T

A

A

L

V

A

A

K

K

V

V

T

T

G

G

T

T

L

L

D

D

F

Y

G

G

A

A

D

D

L

L

G

G

I

L

K

K

M

M

P

P

E

A

E

G

T

T

L

L

R

H

C

L

A

A

L

M

V

V

Q

Q

A

A

E

K

A

V

S

S

H

H

A

A

K

N

I

I

L

K

G

G

I

I

D

D

T

T

S

S

E

E

A

A

E

L

K

T

M

M

P

P

G

G

V

V

F

H

K

S

V

V

V

I

T

T

H

H

K

K

D

D

I

V

K

K

G

G

K

K

N

N

R

R

I

I

T

T

G

G

L

L

I
|
I

T

T

F

F

P

P

S

T

N

N

K

K

G

G

D

D

G

G

W

W

D

D

R

R

P

P

I

I

L

L

C

C

D

D

E

E

K

K

V

V

F

F

Q

Q

Y

Y

G

G

D

D

A

C

I

I

A

A

I

L

V

V

C

C

A

A

D

D

T

S

E

E

E

A

H

N

A

A

K

R

A

A

A

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A

K

V

V

K

K

V

V

D

D

L

L

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E

V

E

L

L

P

P

A

A

Y

Y

M

M

S

S

A

G

P

P

A

A

M

A

A

A

D

E

D

D

A

A

M

I

E

E

I

I

H

H

P

P

G

G

T

T

P

P

N

N

V

V

Y

Y

F

F

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E

Q

Q

H

P

L

I

V

V

K

K

G

G

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T

K

G

P

P

I

I

F

F

D

A

K

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A

A

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D

V

V

V

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V

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G

D

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F

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Y

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V

G

G

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Q

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M

M

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I

I

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D

D

V

V

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A

F

F

A

A

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M

N

G

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D

D

D

G

G

K

K

L

C

C

Y

I

I

H

H

S

S

K

K

S

S

I
|
I

G

G

L

V

H

H

L
|
L

H

H

L

L

Y

Y

M

M

I

I

A

A

P

P

G

G

L

V

G

G

I

L

E

E

P

P

D

D

N

Q

I

L

I

V

M

L

V

V

Q

A

N

N

P

P

T

M

G

G

T
|
T

F
|
F

G
|
G

Y

Y

K

K

F
|
F

S

S

P

P

T

T

M

S

E

E

A

A

L

L

V

V

G

A

A

V

A

A

M

M

A

A

T

T

G

G

R

R

P

P

V

V

F

H

L

L

N

R

Y

Y

S

N

W

Y

Y

Q

Q

Q

T

Q

Y

Y

T

T

G

G

K

K

R
|
R

S

S

P

P

F

W

F

E

I

M

N

N

L

V

R

K

Y

F

A

A

T

K

R

K

E

D

G

G

K

T

L

L

A

A

M

M

E

E

S

S

D

D

W

W

S

L

V

V

D

D

H

H

G

G

P

P

Y

Y

S

S

E

E

F

F

G

G

D

D

L
|
L

T

T

L

L

R
|
R

G

G

A

A

Q

Q

F
|
F

I

I

G

G

A

A

G

G

Y

Y

D

N

I

I

P

P

S

N

I

I

R

R

G

G

L

L

G

G

R

R

T

T

V

V

C

A

T

T

N

N

H

H

A

V

W

W

G

G

S

S

A
|
A

F
|
F

R
|
R

G

G

Y
|
Y

G

G

S

A

P

P

Q
|
Q

S

S

E

M

F

F

A

A

S

S

E

E

V

C

L

L

M

M

D

D

E

M

L

L

A

A

E

E

K

K

L

L

G

G

I

M

D

D

P

P

L

L

E

E

L

L

R

R

Y

Y

R

K

N

N

V

A

Y

Y

R

R

E

P

G

G

A

D

T

T

T

N

P

P

T

T

G

G

Q

Q

V

E

P

P

E

E

V

V

L

F

S

S

L

L

P

P

E

D

L

M

L

I

D

D

T

Q

A

L

R

R

P

P

R

K

Y

Y

L

Q

A

A

K

L

E

E

K

K

A

A

A

Q

R

K

E

E

S

S

T

T

A

A

E

T

V

H

K

K

G

G

V

V

G

G

I

I

S

S

V

I

G

G

V

V

Y

Y

G

G

C

S

G

G

L
|
L

D

D

G

G

P

P

D

D

T

A

A

S

E

E

I

A

A

W

V

A

E

E

L

L

N

N

E

A

D

D

G

G

T

T

V

I

T

T

I

V

F

H

A

T

T

A

W

W

H

E

D

D

H
|
H

G
|
G

Q
|
Q

G
|
G

A

A

D

D

M

I

G

G

T

C

L

V

G

G

T

T

A

A

H

H

E

E

A

A

L

L

R

R

P

P

L

M

G

G

I

V

A

A

P

P

E

E

N

K

I

I

R

K

L

F

V

T

L

W

N

P

D

N

T

T

A

A

V

T

C

T

P

P

N

N

A

S

G

G

P
|
P

A
x
S

G
|
G

S

S

R

R

S
x
Q

Q
|
Q

V

V

V

M

V

T

G

G

R

N

A

A

I

I

K

R

A

V

G

A

C

C

E

E

L

N

L

L

G

K

G

A

M

C

R

E

K

K

A

P

D

G

G

G

T

G

F

Y

R

Y

T

T

Y

Y

A

D

E

E

M

L

R

K

D

A

E

A

K

D

I

K

A

P

T

T

R

K

Y

I

S

T

G

G

K

N

W

W

S

T

A

A

P

S

-

G

C

A

T

T

D

H

C

C

N

D

A

A

-

V

E

T

G

G

Q

L

G

G

S

K

P

P

F

F

A

V

V

V

Y

Y

M

M

Y

Y

G

G

V

V

F

F

L

M

A

A

E

E

V

V

S

T

V

V

E

D

L

V

A

A

T

T

G

G

K

Q

T

T

V

V

D

D

K

G

M

M

T

T

L

L

V

M

A

A

D

D

I

L

G

G

K

S

I

L

V
x
C

N
|
N

R

Q

L

L

T

A

V
x
T

D

D

G

G

Q
|
Q

L

I

Y

Y

G

G

I

L

A

A

Q

Q

G

G

I

I

G

G

L

L

A

A

L

L

S

S

E

E

D

D

F

F

E

E

D

D

I

I

K

K

H

H

S

A

T

T

M

L

P

V

G

G

A

A

G

G

F

F

P

P

Y

F

I

I

K

K

Q

Q

I

I

P

P

D

D

E

K

I

L

D

D

L

I

V

V

Y

Y

V

V

E

N

V

H

P

P

R

R

P

P

E

D

G

G

P

P

F

F

G

G

A

A

G
x
S

G
|
G

V
|
V

G
|
G

E
|
E

L
|
L

P

P

L

L

T

T

C

S

P

P

H

H

A

A

I

I

N

N

A

A

I

I

H

K

N

S

A

A

C

T

G

G

V

V

R

R

V

I

T

Y

R

R

L

L

P

P

A

A

L

Y

P

P

E

E

K

K

V

V

L

L

A

E

G

A

L

L

Q

K

active site: I390 (= I390), F425 (= F425), R501 (= R501), F505 (= F505), R533 (= R533), E869 (= E867), L870 (= L868)
binding bicarbonate ion: R460 (= R460), L498 (= L498), A531 (= A531), F532 (= F532), Y535 (= Y535), Q539 (= Q539)
binding fe2/s2 (inorganic) cluster: V38 (= V38), C40 (= C40), E41 (≠ G41), G43 (= G43), C45 (= C45), G46 (= G46), C48 (= C48), R58 (= R58), C60 (= C60), C100 (= C100), G101 (= G101), C103 (= C103), C137 (= C137), C139 (= C139)
binding benzaldehyde: I255 (= I255), I255 (= I255), L394 (= L394), F425 (= F425), F425 (= F425), F425 (= F425), F425 (= F425), L497 (= L497), L497 (= L497), R501 (= R501), A531 (= A531), Y535 (= Y535), Y535 (= Y535), L626 (= L626), L626 (= L626), L626 (= L626), P694 (= P694), G696 (= G696), G697 (= G697)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q99), C139 (= C139), T420 (= T420), F421 (= F421), G422 (= G422), R533 (= R533), H653 (= H653), G654 (= G654), Q655 (= Q655), G656 (= G656), S695 (≠ A695), G696 (= G696), G697 (= G697), Q700 (≠ S700), Q701 (= Q701), C799 (≠ V797), N800 (= N798), T804 (≠ V802), Q807 (= Q805), S865 (≠ G863), G866 (= G864), V867 (= V865), G868 (= G866), E869 (= E867)

4c7yA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with sodium dithionite and sodium sulfide (see paper)
70% identity, 100% coverage: 1:904/906 of query aligns to 1:906/907 of 4c7yA

query
sites
4c7yA

M

M

I

I

K

Q

R

K

M

V

V

I

T

T

I

V

N

N

G

G

A

I

P

E

R

Q

M

N

A

L

I

F

T

V

R

D

P

A

D

E

T

A

T

L

L

L

A

S

T

D

Y

V

L

L

R

R

E

Q

S

Q

L

L

G

G

L

L

T

T

S

G

V

V

K

K

V

V

G

G

C
|
C

G
x
E

Q

Q

G
|
G

H

Q

C
|
C

G
|
G

S

A

C
|
C

N

S

V

V

I

I

V

L

D

D

G

G

K

K

L

V

V

V

R
|
R

S

A

C
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