SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 207038 FitnessBrowser__DvH:207038 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

5ygeA Arga complexed with acecoa and glutamate (see paper)
36% identity, 77% coverage: 3:121/154 of query aligns to 5:123/169 of 5ygeA

query
sites
5ygeA

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binding acetyl coenzyme *a: Y25 (≠ C23), I73 (= I71), V76 (≠ L74), A77 (≠ V75), V78 (= V76), T83 (≠ R81), G84 (= G82), H85 (≠ Q83), G86 (= G84), G88 (= G86), H89 (≠ R87), T111 (= T109), E113 (≠ Q111), F117 (= F115)
binding glutamic acid: K33 (≠ R32), E44 (≠ D43), H64 (≠ S62), R74 (= R72)

Sites not aligning to the query:

binding acetyl coenzyme *a: 1, 2

6addA The crystal structure of rv2747 from mycobacterium tuberculosis in complex with coa and nlq (see paper)
36% identity, 77% coverage: 3:121/154 of query aligns to 4:122/168 of 6addA

query
sites
6addA

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binding coenzyme a: Y24 (≠ C23), I28 (≠ L28), V75 (≠ L74), A76 (≠ V75), V77 (= V76), T82 (≠ R81), G83 (= G82), H84 (≠ Q83), G85 (= G84), G87 (= G86), H88 (≠ R87), T110 (= T109), E112 (≠ Q111), F115 (= F114)
binding n~2~-acetyl-l-glutamine: K27 (≠ R27), I28 (≠ L28), L30 (= L30), T74 (≠ S73), L109 (= L108)

Sites not aligning to the query:

5yo2A The crystal structure of rv2747 from mycobacterium tuberculosis in complex with acetyl coa and l-arginine (see paper)
36% identity, 77% coverage: 3:121/154 of query aligns to 4:122/168 of 5yo2A

query
sites
5yo2A

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binding acetyl coenzyme *a: Y24 (≠ C23), I28 (≠ L28), I72 (= I71), R73 (= R72), T74 (≠ S73), V75 (≠ L74), V77 (= V76), T82 (≠ R81), G83 (= G82), H84 (≠ Q83), G85 (= G84), G87 (= G86), H88 (≠ R87), T110 (= T109), E112 (≠ Q111), F115 (= F114), F116 (= F115)
binding arginine: G26 (= G25), L29 (= L29), L30 (= L30), R73 (= R72), L109 (= L108)

Sites not aligning to the query:

binding arginine: 147

4i49A Structure of ngnags bound with bisubstrate analog coa-NAG (see paper)
31% identity, 85% coverage: 5:135/154 of query aligns to 277:407/424 of 4i49A

query
sites
4i49A

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binding (2S)-2-({(3S,5R,9R)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9-trihydroxy-8,8-dimethyl-3,5-dioxido-10,14,20-trioxo-2,4,6-trioxa-18-thia-11,15-diaza-3lambda~5~,5lambda~5~-diphosphaicosan-20-yl}amino)pentanedioic acid (non-preferred name): I300 (≠ L28), L301 (= L29), L302 (= L30), R304 (= R32), A343 (≠ R72), C344 (≠ S73), L345 (= L74), A346 (≠ V75), V347 (= V76), Q352 (≠ R81), D353 (≠ G82), G355 (= G84), G357 (= G86), E358 (≠ R87), L379 (= L108), S380 (≠ T109), T383 (≠ V112), W386 (≠ F114), F387 (= F115)

Sites not aligning to the query:

binding (2S)-2-({(3S,5R,9R)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9-trihydroxy-8,8-dimethyl-3,5-dioxido-10,14,20-trioxo-2,4,6-trioxa-18-thia-11,15-diaza-3lambda~5~,5lambda~5~-diphosphaicosan-20-yl}amino)pentanedioic acid (non-preferred name): 413, 415

3d2mA Crystal structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and l-glutamate (see paper)
31% identity, 85% coverage: 5:135/154 of query aligns to 277:407/424 of 3d2mA

query
sites
3d2mA

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binding coenzyme a: L345 (= L74), V347 (= V76), Q352 (≠ R81), D353 (≠ G82), G355 (= G84), G357 (= G86), E358 (≠ R87), S380 (≠ T109), T383 (≠ V112), W386 (≠ F114)
binding glutamic acid: L302 (= L30), R304 (= R32), A343 (≠ R72), C344 (≠ S73), L379 (= L108)

Sites not aligning to the query:

active site: 26
binding glutamic acid: 413, 415

3b8gA Crysta structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and n-acetyl-glutamate (see paper)
31% identity, 85% coverage: 5:135/154 of query aligns to 277:407/424 of 3b8gA

query
sites
3b8gA

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binding coenzyme a: I300 (≠ L28), L345 (= L74), V347 (= V76), Q352 (≠ R81), D353 (≠ G82), G355 (= G84), Y356 (≠ W85), G357 (= G86), E358 (≠ R87), S380 (≠ T109), T383 (≠ V112), E385 (= E113), W386 (≠ F114)
binding n-acetyl-l-glutamate: L302 (= L30), R304 (= R32), A343 (≠ R72), C344 (≠ S73), L379 (= L108)

Sites not aligning to the query:

active site: 26
binding n-acetyl-l-glutamate: 413, 415

2r8vA Native structure of n-acetylglutamate synthase from neisseria gonorrhoeae (see paper)
31% identity, 85% coverage: 5:135/154 of query aligns to 277:407/424 of 2r8vA

query
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2r8vA

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binding acetyl coenzyme *a: I300 (≠ L28), L301 (= L29), L345 (= L74), V347 (= V76), Q352 (≠ R81), D353 (≠ G82), G355 (= G84), G357 (= G86), E358 (≠ R87), T383 (≠ V112), E385 (= E113), W386 (≠ F114), F387 (= F115)

Sites not aligning to the query:

active site: 26

3d2pA Crystal structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and l-arginine (see paper)
30% identity, 85% coverage: 5:135/154 of query aligns to 285:411/424 of 3d2pA

query
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3d2pA

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K

I

T

T

F

W

A

E

E

-

A

D

D

I

C

A

G

E

E

I

I

R

A

S

C

L
|
L

V

A

V
|
V

L

S

E

P

S

Q

L

A

R
x
Q

G
x
D

Q

G

G
|
G

W

Y

G
|
G

R
x
E

R

R

L

L

V

L

E

A

A

H

C

I

M

I

S

D

D

K

A

A

V

R

T

G

L

I

G

G

L

I

Y

S

R

R

V

L

F

F

T

A

L

L

T

S

Y

T

Q

N

V

T

-

G

E
|
E

F
x
W

F

F

N

A

K

E

L

R

G

G

Y

F

S

Q

V

T

V

A

G

S

K

E

E

D

V

E

L

L

P

P

Q

E

K

T

V

R

W

R

A

K

D

D

binding coenzyme a: L303 (≠ C23), L349 (= L74), V351 (= V76), Q356 (≠ R81), D357 (≠ G82), G359 (= G84), G361 (= G86), E362 (≠ R87), E389 (= E113), W390 (≠ F114)

Sites not aligning to the query:

active site: 26
binding arginine: 13, 197, 216, 253, 266, 267, 269, 270, 271, 274, 276

P0A6C5 Amino-acid acetyltransferase; N-acetylglutamate synthase; AGS; NAGS; EC 2.3.1.1 from Escherichia coli (strain K12) (see paper)
28% identity, 84% coverage: 2:130/154 of query aligns to 295:423/443 of P0A6C5

query
sites
P0A6C5

A

A

P

E

F

Q

I

I

R

R

K

R

A

A

T

T

V

I

P

N

D

D

V

I

K

G

R

G

I

I

H

L

A

E

I

L

L

I

M

R

E

P

C

L

A

E

G

Q

Q

Q

R

G

L

I

L

L

L

V

P

R

R

R

S

S

L

R

N

E

D

Q

L

L

Y

E

S

M

R

E

I

I

R

D

D

K

F

F

V

T

V

I

V

I

D

Q

A

R

D

D

E

-

G

-

G

N

A

T

I

T

L

I

G

A

C

C

C

A

A

A

L

L

-

Y

S

P

I

F

T

P

W

E

E

E

D

K

I

I

A

G

E

E

I

M

R

A

S

C

L

V

V

A

V

V

L

H

E

P

S

D

L

Y

R

R

G

S

Q

S

G

S

W

R

G

G

R

E

R

V

L

L

V

L

E

E

A

R

C

I

M

A

S

A

D

Q

A

A

V

K

T

Q

L

S

G

G

L

L

Y

S

R

K

V

L

F

F

T

V

L

L

T

T

Y

T

Q

R

-

S

V

I

E

H

F

W

F

F

N

Q

K

E

L

R

G

G

Y

F

S

T

V

P

V

V

G

D

K

I

E

D

V

L

L

L

P

P

Q

E

Sites not aligning to the query:

15 H→Y: In EE17.
19 Y→C: In EE51.
54 S→N: In PT2M217.
58 R→H: In EE11.
287 G→S: In PT2M216.
432 Q→R: In PT2M217.

Query Sequence

>207038 FitnessBrowser__DvH:207038
MAPFIRKATVPDVKRIHAILMECAGQRLLLPRSLNDLYSRIRDFVVVDADEGGAILGCCA
LSITWEDIAEIRSLVVLESLRGQGWGRRLVEACMSDAVTLGLYRVFTLTYQVEFFNKLGY
SVVGKEVLPQKVWADCIHCPQFPECDETAMLIEL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory