SitesBLAST
Comparing 207114 DVU1655 aminotransferase, classes I and II to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2x5dD Crystal structure of a probable aminotransferase from pseudomonas aeruginosa (see paper)
40% identity, 94% coverage: 23:388/388 of query aligns to 7:374/380 of 2x5dD
- active site: Y113 (= Y129), D191 (= D207), A193 (= A209), K225 (= K242)
- binding pyridoxal-5'-phosphate: Y51 (= Y67), G87 (= G103), S88 (= S104), K89 (= K105), Y113 (= Y129), N163 (= N179), D191 (= D207), A193 (= A209), Y194 (= Y210), T222 (≠ S239), S224 (= S241), K225 (= K242), R233 (= R250), Y256 (≠ S273)
6l1oA Product bound bacf structure from bacillus subtillis (see paper)
37% identity, 98% coverage: 7:388/388 of query aligns to 6:387/393 of 6l1oA
- binding pyridoxal-5'-phosphate: G102 (= G103), G103 (≠ S104), K104 (= K105), Y128 (= Y129), N174 (= N175), N178 (= N179), D206 (= D207), A208 (= A209), Y209 (= Y210), S236 (= S239), S238 (= S241), K239 (= K242), R247 (= R250)
- binding tyrosine: Y66 (= Y67), F69 (≠ Y70), V270 (≠ S273)
6l1lB Apo-bacf structure from bacillus subtillis (see paper)
37% identity, 98% coverage: 7:388/388 of query aligns to 6:387/393 of 6l1lB
- binding pyridoxal-5'-phosphate: G102 (= G103), G103 (≠ S104), K104 (= K105), Y128 (= Y129), N174 (= N175), N178 (= N179), D206 (= D207), A208 (= A209), Y209 (= Y210), S236 (= S239), S238 (= S241), K239 (= K242), R247 (= R250)
6l1oB Product bound bacf structure from bacillus subtillis (see paper)
37% identity, 98% coverage: 7:388/388 of query aligns to 6:387/392 of 6l1oB
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G102 (= G103), G103 (≠ S104), K104 (= K105), Y128 (= Y129), N174 (= N175), N178 (= N179), D206 (= D207), A208 (= A209), Y209 (= Y210), S236 (= S239), S238 (= S241), K239 (= K242), R247 (= R250)
- binding tyrosine: F17 (= F18), Q39 (≠ I40), G40 (= G41), K104 (= K105), Y128 (= Y129), E130 (≠ V131), K239 (= K242), Y325 (= Y327), R367 (= R368)
2o1bA Structure of aminotransferase from staphylococcus aureus
38% identity, 91% coverage: 35:388/388 of query aligns to 21:371/376 of 2o1bA
- active site: Y115 (= Y129), D192 (= D207), A194 (= A209), K225 (= K242)
- binding pyridoxal-5'-phosphate: G89 (= G103), T90 (≠ S104), K91 (= K105), L94 (≠ I108), Y115 (= Y129), N164 (= N179), D192 (= D207), A194 (= A209), Y195 (= Y210), S222 (= S239), S224 (= S241), K225 (= K242), R233 (= R250)
6l1nA Substrate bound bacf structure from bacillus subtillis (see paper)
37% identity, 98% coverage: 7:388/388 of query aligns to 6:386/393 of 6l1nA
- binding glycine: E16 (≠ L17), Q38 (≠ I40), G39 (= G41), Y127 (= Y129), N177 (= N179), Y208 (= Y210), K238 (= K242), Y324 (= Y327), R366 (= R368)
- binding pyridoxal-5'-phosphate: G101 (= G103), G102 (≠ S104), K103 (= K105), Y127 (= Y129), N173 (= N175), N177 (= N179), D205 (= D207), A207 (= A209), Y208 (= Y210), S235 (= S239), S237 (= S241), K238 (= K242), R246 (= R250)
1gdeA Crystal structure of pyrococcus protein a-1 e-form (see paper)
33% identity, 93% coverage: 27:388/388 of query aligns to 19:381/388 of 1gdeA
- active site: K232 (= K242)
- binding glutamic acid: G33 (= G41), F120 (≠ Y129), N170 (= N179), K232 (= K242), Y319 (= Y327), R361 (= R368)
- binding pyridoxal-5'-phosphate: G94 (= G103), A95 (≠ S104), N96 (≠ K105), F120 (≠ Y129), N166 (= N175), N170 (= N179), D198 (= D207), V200 (≠ A209), Y201 (= Y210), S231 (= S241), K232 (= K242), R240 (= R250)
1gd9A Crystall structure of pyrococcus protein-a1 (see paper)
33% identity, 93% coverage: 27:388/388 of query aligns to 19:381/388 of 1gd9A
- active site: K232 (= K242)
- binding pyridoxal-5'-phosphate: G94 (= G103), A95 (≠ S104), N96 (≠ K105), F120 (≠ Y129), N166 (= N175), N170 (= N179), D198 (= D207), V200 (≠ A209), Y201 (= Y210), S231 (= S241), K232 (= K242), R240 (= R250)
3jtxB Crystal structure of aminotransferase (np_283882.1) from neisseria meningitidis z2491 at 1.91 a resolution
32% identity, 98% coverage: 10:388/388 of query aligns to 5:391/393 of 3jtxB
Q02635 Aspartate/prephenate aminotransferase; AspAT / PAT; Transaminase A; EC 2.6.1.1; EC 2.6.1.79 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) (see paper)
30% identity, 99% coverage: 6:388/388 of query aligns to 4:395/400 of Q02635
- K12 (≠ P14) Important for prephenate aminotransferase activity; mutation to G: 7-fold increase in Km for prephenate. Weakly affects Km for oxaloacetate.
6f77A Crystal structure of the prephenate aminotransferase from rhizobium meliloti (see paper)
30% identity, 99% coverage: 6:388/388 of query aligns to 3:394/399 of 6f77A
- binding pyridoxal-5'-phosphate: G98 (= G103), G99 (≠ S104), K100 (= K105), W124 (≠ Y129), Y127 (= Y132), N170 (= N175), N174 (= N179), D203 (= D207), M205 (≠ A209), Y206 (= Y210), S237 (= S241), K238 (= K242), R246 (= R250)
Q56232 Aspartate/prephenate aminotransferase; AspAT / PAT; Transaminase A; EC 2.6.1.1; EC 2.6.1.78 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see 3 papers)
28% identity, 99% coverage: 6:388/388 of query aligns to 4:381/385 of Q56232