SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 207248 MicrobesOnline__882:207248 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

5odqB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with bromoethanesulfonate. (see paper)
24% identity, 81% coverage: 8:210/250 of query aligns to 1:224/291 of 5odqB

query
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5odqB

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G15), C9 (= C17), C41 (= C49), C42 (= C50), C78 (≠ S85), G80 (≠ S87), C81 (= C88), G152 (≠ S145), C153 (= C146), H154 (= H147), C193 (= C179), C194 (= C180)
binding 2-bromanylethanesulfonic acid: G46 (≠ A54), G80 (≠ S87), H154 (= H147), G197 (= G183), R201 (≠ S187)

Sites not aligning to the query:

5odhH Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
24% identity, 81% coverage: 8:210/250 of query aligns to 1:224/291 of 5odhH

query
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5odhH

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G15), C9 (= C17), C41 (= C49), C42 (= C50), C78 (≠ S85), G80 (≠ S87), C81 (= C88), C153 (= C146), H154 (= H147), C193 (= C179), C194 (= C180)
binding 1-thioethanesulfonic acid: A44 (≠ Q52), P45 (= P53), G46 (≠ A54), G80 (≠ S87), H154 (= H147), G197 (= G183), G198 (= G184)
binding Coenzyme B: R201 (≠ S187)

Sites not aligning to the query:

5odhB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
24% identity, 81% coverage: 8:210/250 of query aligns to 1:224/291 of 5odhB

query
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5odhB

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G15), C9 (= C17), I10 (≠ L18), C41 (= C49), C42 (= C50), C78 (≠ S85), G80 (≠ S87), C81 (= C88), G152 (≠ S145), C153 (= C146), H154 (= H147), C193 (= C179), C194 (= C180)
binding 1-thioethanesulfonic acid: P45 (= P53), G46 (≠ A54), G80 (≠ S87)

Sites not aligning to the query:

5odcB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
24% identity, 81% coverage: 8:210/250 of query aligns to 1:224/291 of 5odcB

query
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5odcB

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G15), C9 (= C17), I10 (≠ L18), C41 (= C49), C42 (= C50), C78 (≠ S85), N79 (≠ G86), G80 (≠ S87), C81 (= C88), G152 (≠ S145), C153 (= C146), C193 (= C179), C194 (= C180), G197 (= G183)

Sites not aligning to the query:

binding Non-cubane [4Fe-4S]-cluster: 231, 233, 234

Query Sequence

>207248 MicrobesOnline__882:207248
MYTYPEDIKTVYFFGTCLVDMSFPDAGMAGIRLLQRAGVEVVFPAAQSCCGQPAYNSGFF
DEARTVALRQVEAFSAHDWPIVVPSGSCAGMMRFHYPELFAEAPDYFEVRRFSERVFELG
DFLCNALHQTYEDKGAPIKVTWHSSCHAMREMGAVAAAKTLIGQLANVELVDLPYEYECC
GFGGTFSIKQPELSGAMVSDKVANIRSTGAARVLSGDCGCLMNINGAMEKQSVPVQGQHL
ASFLWERING

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory