SitesBLAST
Comparing 207304 MicrobesOnline__882:207304 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5uwyA The crystal structure of thioredoxin reductase from streptococcus pyogenes mgas5005
46% identity, 98% coverage: 4:305/307 of query aligns to 5:306/306 of 5uwyA
- binding flavin-adenine dinucleotide: G13 (= G12), P14 (= P13), A15 (= A14), E34 (= E33), Q35 (≠ R34), G40 (= G39), Q41 (= Q40), T45 (= T44), N50 (= N49), V82 (= V82), T110 (≠ S110), G111 (= G111), Y114 (= Y114), C136 (= C136), V242 (= V241), G275 (= G274), D276 (= D275), Q284 (= Q283), I285 (≠ V284)
5mh4A Crystal structure of lactococcus lactis thioredoxin reductase (fr conformation) (see paper)
44% identity, 99% coverage: 3:305/307 of query aligns to 1:303/303 of 5mh4A
- active site: V34 (≠ S36), P35 (= P37), M39 (≠ V41), E44 (= E46), C130 (= C133), C133 (= C136), D134 (= D137)
- binding flavin-adenine dinucleotide: G10 (= G12), P11 (= P13), A12 (= A14), E31 (= E33), R32 (= R34), G37 (= G39), Q38 (= Q40), T42 (= T44), N47 (= N49), G77 (≠ D80), V79 (= V82), T107 (≠ S110), G108 (= G111), E155 (= E158), V239 (= V241), F242 (= F244), G272 (= G274), D273 (= D275), Q281 (= Q283), I282 (≠ V284)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I146 (≠ V149), G147 (= G150), G148 (= G151), G149 (= G152), D150 (≠ N153), S151 (= S154), E154 (= E157), H170 (= H173), R171 (= R174), R172 (= R175), R176 (= R179), V234 (≠ I236), G235 (= G237), R280 (= R282), Q281 (= Q283)
7aawB Thioredoxin reductase from bacillus cereus (see paper)
40% identity, 98% coverage: 4:305/307 of query aligns to 4:305/311 of 7aawB
- binding flavin-adenine dinucleotide: G10 (= G10), G12 (= G12), P13 (= P13), A14 (= A14), E33 (= E33), R34 (= R34), G39 (= G39), Q40 (= Q40), T44 (= T44), N49 (= N49), G79 (≠ D80), D80 (≠ E81), V81 (= V82), S109 (= S110), G110 (= G111), Y113 (= Y114), C135 (= C136), G274 (= G274), D275 (= D275), Q283 (= Q283), I284 (≠ V284)
- binding alpha-D-glucopyranose: D46 (≠ E46), E48 (= E48), G126 (= G127), G128 (= G129), D136 (= D137), A138 (≠ N139), F139 (= F140), F139 (= F140), F140 (= F141)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G150 (= G151), G151 (= G152), D152 (≠ N153), S153 (= S154), E156 (= E157), H172 (= H173), R173 (= R174), R174 (= R175), R178 (= R179), I236 (= I236)
7aawA Thioredoxin reductase from bacillus cereus (see paper)
40% identity, 98% coverage: 4:305/307 of query aligns to 7:308/315 of 7aawA
- binding flavin-adenine dinucleotide: G13 (= G10), G15 (= G12), P16 (= P13), A17 (= A14), E36 (= E33), R37 (= R34), G42 (= G39), Q43 (= Q40), T47 (= T44), N52 (= N49), G82 (≠ D80), V84 (= V82), A111 (≠ C109), S112 (= S110), G113 (= G111), C138 (= C136), G277 (= G274), D278 (= D275), Q286 (= Q283), I287 (≠ V284)
- binding alpha-D-glucopyranose: R27 (= R24), D49 (≠ E46), K74 (≠ G72), F75 (≠ Y73), P122 (= P120), G123 (≠ D121), E126 (≠ R124), G129 (= G127), G131 (= G129), V132 (= V130), F143 (= F141), E206 (≠ A204), N208 (≠ H206)
4gcmB Crystal structure of a thioredoxine reductase (trxb) from staphylococcus aureus subsp. Aureus mu50 at 1.80 a resolution
40% identity, 99% coverage: 4:306/307 of query aligns to 5:307/309 of 4gcmB
- active site: C133 (= C133), C136 (= C136), D137 (= D137)
- binding flavin-adenine dinucleotide: G11 (= G10), G13 (= G12), P14 (= P13), A15 (= A14), E34 (= E33), R35 (= R34), G40 (= G39), Q41 (= Q40), T45 (= T44), N50 (= N49), D81 (= D80), I82 (≠ V82), T110 (≠ S110), G111 (= G111), C136 (= C136), G275 (= G274), D276 (= D275), Q284 (= Q283), I285 (≠ V284)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I117 (≠ L117), G151 (= G151), G152 (= G152), D153 (≠ N153), S154 (= S154), E157 (= E157), R174 (= R174), R175 (= R175), R179 (= R179), Q181 (≠ A181), I237 (= I236)
2q7vA Crystal structure of deinococcus radiodurans thioredoxin reductase (see paper)
41% identity, 96% coverage: 2:295/307 of query aligns to 6:303/313 of 2q7vA
- active site: P41 (= P37), I45 (≠ V41), E50 (= E46), C141 (= C133), C144 (= C136), D145 (= D137)
- binding flavin-adenine dinucleotide: G16 (= G12), P17 (= P13), A18 (= A14), E37 (= E33), K38 (≠ R34), G43 (= G39), Q44 (= Q40), I45 (≠ V41), N53 (= N49), E85 (= E81), V86 (= V82), T118 (≠ S110), G119 (= G111), C144 (= C136), G282 (= G274), D283 (= D275), Q291 (= Q283), L292 (≠ V284), S295 (≠ A287)
7jypA Structure of thioredoxin reductase from the thermophilic eubacterium thermosipho africanus tcf52b
40% identity, 96% coverage: 4:298/307 of query aligns to 6:300/305 of 7jypA
- binding flavin-adenine dinucleotide: G12 (= G10), G14 (= G12), P15 (= P13), A16 (= A14), F34 (≠ V32), E35 (= E33), K36 (≠ R34), G41 (= G39), A42 (≠ Q40), V83 (= V82), T112 (≠ S110), G113 (= G111), G276 (= G274), D277 (= D275)
- binding nicotinamide-adenine-dinucleotide: G153 (= G151), G154 (= G152), S156 (= S154), Q175 (≠ H173), N176 (≠ R174), T181 (≠ R179), V238 (≠ I236)
5uthA Crystal structure of thioredoxin reductase from mycobacterium smegmatis in complex with fad
41% identity, 98% coverage: 5:304/307 of query aligns to 5:305/306 of 5uthA
- active site: C133 (= C133), C136 (= C136), D137 (= D137)
- binding flavin-adenine dinucleotide: I9 (= I9), G10 (= G10), S11 (≠ G11), G12 (= G12), P13 (= P13), A14 (= A14), F32 (≠ V32), E33 (= E33), G34 (≠ R34), Q36 (≠ S36), G39 (= G39), A40 (≠ Q40), L41 (≠ V41), N49 (= N49), D81 (≠ E81), V82 (= V82), M110 (≠ S110), G111 (= G111), C136 (= C136), G275 (= G274), D276 (= D275), R283 (= R282), Q284 (= Q283), A285 (≠ V284), A288 (= A287)
8ccmA Crystal structure of mycobacterium smegmatis thioredoxin reductase in complex with compound 2-06
41% identity, 98% coverage: 5:304/307 of query aligns to 4:304/305 of 8ccmA
- binding flavin-adenine dinucleotide: I8 (= I9), G9 (= G10), S10 (≠ G11), G11 (= G12), P12 (= P13), A13 (= A14), E32 (= E33), G33 (≠ R34), Q35 (≠ S36), G38 (= G39), A39 (≠ Q40), L40 (≠ V41), T43 (= T44), N48 (= N49), D80 (≠ E81), V81 (= V82), M109 (≠ S110), G110 (= G111), T131 (≠ Y132), C135 (= C136), G274 (= G274), D275 (= D275), R282 (= R282), Q283 (= Q283), A284 (≠ V284), A287 (= A287)
- binding ~{N}6-(4-aminophenyl)-1,2-benzothiazole-3,6-diamine: R114 (≠ K115), H115 (≠ R116), L116 (= L117), R173 (= R174), E200 (≠ V201), I201 (≠ V202), I235 (= I236)
8cclA Crystal structure of mycobacterium smegmatis thioredoxin reductase in complex with fragment f2x-entry a09
41% identity, 98% coverage: 5:304/307 of query aligns to 4:304/305 of 8cclA
- binding flavin-adenine dinucleotide: I8 (= I9), G9 (= G10), S10 (≠ G11), G11 (= G12), P12 (= P13), A13 (= A14), E32 (= E33), G33 (≠ R34), Q35 (≠ S36), G38 (= G39), A39 (≠ Q40), L40 (≠ V41), T43 (= T44), N48 (= N49), D80 (≠ E81), V81 (= V82), M109 (≠ S110), G110 (= G111), T131 (≠ Y132), C135 (= C136), G274 (= G274), D275 (= D275), R282 (= R282), Q283 (= Q283), A284 (≠ V284), A287 (= A287)
- binding [1,2]thiazolo[5,4-b]pyridin-3-amine: L116 (= L117), R173 (= R174), E200 (≠ V201), I201 (≠ V202)
8cckA Crystal structure of mycobacterium smegmatis thioredoxin reductase in complex with fragment f2x-entry h07
41% identity, 98% coverage: 5:304/307 of query aligns to 4:304/305 of 8cckA
- binding flavin-adenine dinucleotide: G9 (= G10), S10 (≠ G11), G11 (= G12), P12 (= P13), A13 (= A14), E32 (= E33), G33 (≠ R34), Q35 (≠ S36), G38 (= G39), A39 (≠ Q40), L40 (≠ V41), T43 (= T44), N48 (= N49), D80 (≠ E81), V81 (= V82), M109 (≠ S110), G110 (= G111), T131 (≠ Y132), C135 (= C136), G274 (= G274), D275 (= D275), R282 (= R282), Q283 (= Q283), A284 (≠ V284), A287 (= A287)
- binding ~{N}-(4-hydroxyphenyl)-2-pyrazol-1-yl-ethanamide: R114 (≠ K115), H115 (≠ R116), L116 (= L117), V148 (= V149), R173 (= R174), E200 (≠ V201), I201 (≠ V202)
8ccjA Crystal structure of mycobacterium smegmatis thioredoxin reductase in complex with NADPH
41% identity, 98% coverage: 5:304/307 of query aligns to 4:304/305 of 8ccjA
- binding flavin-adenine dinucleotide: I8 (= I9), G9 (= G10), S10 (≠ G11), G11 (= G12), P12 (= P13), A13 (= A14), E32 (= E33), G33 (≠ R34), Q35 (≠ S36), G38 (= G39), A39 (≠ Q40), L40 (≠ V41), T43 (= T44), N48 (= N49), D80 (≠ E81), V81 (= V82), M109 (≠ S110), G110 (= G111), T131 (≠ Y132), C135 (= C136), G274 (= G274), D275 (= D275), R282 (= R282), Q283 (= Q283), A284 (≠ V284), A287 (= A287)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G150 (= G151), G151 (= G152), D152 (≠ N153), S153 (= S154), E156 (= E157), H172 (= H173), R173 (= R174), R174 (= R175), R178 (= R179), I235 (= I236)
3f8pD Structure of sulfolobus solfataricus trxr-b3 (see paper)
39% identity, 99% coverage: 2:304/307 of query aligns to 5:309/310 of 3f8pD
- active site: C135 (= C133), C138 (= C136), D139 (= D137)
- binding nicotinamide-adenine-dinucleotide: V12 (≠ I9), G13 (= G10), L14 (≠ G11), G15 (= G12), P16 (= P13), A17 (= A14), G36 (≠ E33), T38 (≠ S36), G41 (= G39), Q42 (= Q40), I82 (≠ E81), V83 (= V82), G111 (≠ C109), I112 (≠ S110), G113 (= G111), G277 (= G274), D278 (= D275)
2a87A Crystal structure of m. Tuberculosis thioredoxin reductase (see paper)
41% identity, 98% coverage: 5:304/307 of query aligns to 7:308/313 of 2a87A
- active site: F39 (≠ P37), L43 (≠ V41), D48 (≠ E46), C136 (= C133), C139 (= C136), D140 (= D137)
- binding flavin-adenine dinucleotide: G12 (= G10), S13 (≠ G11), G14 (= G12), P15 (= P13), A16 (= A14), F34 (≠ V32), E35 (= E33), G36 (≠ R34), G40 (= G38), G41 (= G39), A42 (≠ Q40), L43 (≠ V41), T46 (= T44), V49 (≠ I47), N51 (= N49), D83 (= D80), V84 (≠ E81), M113 (≠ S110), C139 (= C136), G278 (= G274), D279 (= D275), R286 (= R282), Q287 (= Q283), A288 (≠ V284), V289 (≠ T285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: L120 (= L117), G155 (= G152), D156 (≠ N153), S157 (= S154), H176 (= H173), R177 (= R174), R178 (= R175), R182 (= R179), I239 (= I236), Y259 (≠ F256), R283 (≠ K279), R286 (= R282)
P9WHH1 Thioredoxin reductase; TR; TRXR; EC 1.8.1.9 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
41% identity, 98% coverage: 5:304/307 of query aligns to 16:317/335 of P9WHH1
- SGPA 22:25 (≠ GGPA 11:14) binding
- Y32 (= Y21) modified: Phosphotyrosine; by PtkA; mutation to A: Significantly reduces phosphorylation.
- 44:51 (vs. 33:40, 50% identical) binding
- N60 (= N49) binding
- V93 (≠ E81) binding
- C145 (= C133) modified: Disulfide link with 148, Redox-active
- C148 (= C136) modified: Disulfide link with 145, Redox-active
- S166 (= S154) binding
- H185 (= H173) binding
- R191 (= R179) binding
- I248 (= I236) binding
- Y268 (≠ F256) binding
- D288 (= D275) binding
- R295 (= R282) binding
- RQAV 295:298 (≠ RQVT 282:285) binding
3f8rA Crystal structure of sulfolobus solfataricus thioredoxin reductase b3 in complex with two NADP molecules (see paper)
40% identity, 99% coverage: 2:304/307 of query aligns to 5:307/308 of 3f8rA
- active site: C133 (= C133), C136 (= C136), D137 (= D137)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V12 (≠ I9), G13 (= G10), L14 (≠ G11), G15 (= G12), P16 (= P13), A17 (= A14), E37 (≠ R34), T38 (≠ S36), G41 (= G39), Q42 (= Q40), I82 (≠ E81), V83 (= V82), G109 (≠ C109), I110 (≠ S110), G111 (= G111), R115 (≠ K115), L117 (= L117), D153 (≠ N153), S154 (= S154), E157 (= E157), R174 (= R174), R175 (= R175), Y184 (= Y184), I236 (= I236), G275 (= G274), D276 (= D275), L282 (≠ C281), G283 (vs. gap), R285 (= R282)
Q70G58 Thioredoxin reductase NTRC; NADPH-dependent thioredoxin reductase C; OsNTRC; EC 1.8.1.9 from Oryza sativa subsp. japonica (Rice) (see 2 papers)
37% identity, 96% coverage: 7:302/307 of query aligns to 73:377/515 of Q70G58
- C203 (= C133) mutation to S: Loss of thioredoxin reductase activity.
- C206 (= C136) mutation to S: Loss of thioredoxin reductase activity.
- A227 (= A155) mutation to G: Reduces activity 30-fold; when associated with E-245 and F-246.
- V245 (≠ H173) mutation to E: Reduces activity 30-fold; when associated with G-227 and F-246.
- R246 (= R174) mutation to F: Reduces activity 30-fold; when associated with G-227 and E-245.
Sites not aligning to the query:
- 440 C→S: Loss of thioredoxin activity.
- 443 C→S: Loss of thioredoxin activity.
3f8dA Structure of sulfolobus solfataricus thioredoxin reductase mutant c147a (see paper)
39% identity, 99% coverage: 2:304/307 of query aligns to 5:307/308 of 3f8dA
- active site: C133 (= C133), A136 (≠ C136), D137 (= D137)
- binding flavin-adenine dinucleotide: V12 (≠ I9), G13 (= G10), L14 (≠ G11), G15 (= G12), P16 (= P13), A17 (= A14), G36 (≠ E33), E37 (≠ R34), T38 (≠ S36), G41 (= G39), Q42 (= Q40), E45 (≠ M43), A46 (≠ T44), V49 (≠ I47), D51 (≠ N49), I82 (≠ E81), V83 (= V82), G109 (≠ C109), I110 (≠ S110), G111 (= G111), C133 (= C133), A136 (≠ C136), G275 (= G274), D276 (= D275), R285 (= R282), Q286 (= Q283), V287 (= V284)
Q39243 Thioredoxin reductase 1, mitochondrial; NADPH-dependent thioredoxin reductase 1; NTR1; NADPH-dependent thioredoxin reductase B; AtNTRB; EC 1.8.1.9 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
42% identity, 97% coverage: 8:305/307 of query aligns to 55:367/375 of Q39243
- SGPA 58:61 (≠ GGPA 11:14) binding
- FE 79:80 (≠ VE 32:33) binding
- IAPGGQ 87:92 (≠ LSPGGQ 35:40) binding
- N101 (= N49) binding
- V134 (= V82) binding
- C189 (= C133) modified: Disulfide link with 192, Redox-active
- C192 (= C136) binding ; modified: Disulfide link with 189, Redox-active
- D337 (= D275) binding
- RQA 344:346 (≠ RQV 282:284) binding
Sites not aligning to the query:
- 1:37 modified: transit peptide, Mitochondrion
1vdcA Structure of NADPH dependent thioredoxin reductase (see paper)
42% identity, 97% coverage: 8:305/307 of query aligns to 10:322/322 of 1vdcA
- active site: C144 (= C133), C147 (= C136), D148 (= D137)
- binding flavin-adenine dinucleotide: G12 (= G10), S13 (≠ G11), G14 (= G12), P15 (= P13), A16 (= A14), F34 (≠ V32), E35 (= E33), G36 (vs. gap), A39 (vs. gap), I42 (≠ L35), G46 (= G39), Q47 (= Q40), L48 (≠ V41), V54 (≠ I47), N56 (= N49), T88 (≠ E81), V89 (= V82), I117 (≠ S110), G118 (= G111), C147 (= C136), G291 (= G274), D292 (= D275), R299 (= R282), Q300 (= Q283), A301 (≠ V284), A304 (= A287)
Query Sequence
>207304 MicrobesOnline__882:207304
MQQFDAIVIGGGPAGMTAALYLARSGVSVAMVERLSPGGQVLMTSEIENYPGFPKGIQGW
ELADLFAAHLEGYAITRFNDEVREIVPAPADNRVRVGDDWISGRTLILCSGARYKRLGLP
DEERLTGKGVSYCALCDGNFFRGQVVGVVGGGNSALEESLYLSKLVKKLHLIHRRDDFRA
AKCYQDKVCIMPDIDVVRSSVVEAIHGDDRLTGVTVRNVKTGETSFLELDGLFIFIGFEP
VGGFLPGGIERDEQGFVITDGEMRTNLPGIFAAGDIRSKMCRQVTTAVGDGATAANAAFV
YLEQLDA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory