SitesBLAST

199 G→D: Disrupts mitochondrial function and results in enlarged mitochondria. Neurons present vacuolar lesions leading to neurodegeneration in the central brain. Results in behavioral defects and shorten lifespan.

8e73S8 qcr9 (see paper)
36% identity, 38% coverage: 83:138/147 of query aligns to 73:136/181 of 8e73S8

query
sites
8e73S8

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binding iron/sulfur cluster: C82 (= C92), I83 (≠ M93), C85 (= C95), K86 (≠ G96), C88 (= C98), C92 (= C102), I97 (≠ L107), C121 (= C123), I122 (≠ T124), Y123 (≠ A125), C124 (= C126), G125 (= G127), F126 (≠ L128), C127 (= C129), C131 (= C133), P132 (= P134), A135 (= A137), I136 (≠ M138)

Sites not aligning to the query:

binding iron/sulfur cluster: 70, 138

5gupH structure of mammalian respiratory supercomplex I1III2IV1 (see paper)
34% identity, 38% coverage: 83:138/147 of query aligns to 68:131/176 of 5gupH

query
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5gupH

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binding iron/sulfur cluster: C77 (= C92), I78 (≠ M93), A79 (≠ H94), C80 (= C95), K81 (≠ G96), C83 (= C98), C87 (= C102), C116 (= C123), I117 (≠ T124), Y118 (≠ A125), C119 (= C126), G120 (= G127), F121 (≠ L128), C122 (= C129), C126 (= C133), P127 (= P134), A130 (= A137), I131 (≠ M138)
binding : R108 (≠ T117)

Sites not aligning to the query:

8b6fAL ndutt15 (see paper)
34% identity, 38% coverage: 83:138/147 of query aligns to 109:172/218 of 8b6fAL

query
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8b6fAL

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binding iron/sulfur cluster: C118 (= C92), I119 (≠ M93), C121 (= C95), K122 (≠ G96), C124 (= C98), C128 (= C102), I133 (≠ L107), Y150 (≠ F118), C157 (= C123), I158 (≠ T124), Y159 (≠ A125), C160 (= C126), G161 (= G127), C163 (= C129), C167 (= C133), P168 (= P134), A171 (= A137), I172 (≠ M138)

Sites not aligning to the query:

binding iron/sulfur cluster: 106, 174

6cfwN Cryoem structure of a respiratory membrane-bound hydrogenase (see paper)
33% identity, 41% coverage: 84:143/147 of query aligns to 37:96/121 of 6cfwN

query
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6cfwN

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binding iron/sulfur cluster: C45 (= C92), G47 (≠ H94), C48 (= C95), M50 (= M97), C51 (= C98), C55 (= C102), C76 (= C123), M78 (≠ A125), C79 (= C126), C82 (= C129), C86 (= C133)

6h8kI NUAM protein (see paper)
40% identity, 39% coverage: 90:147/147 of query aligns to 40:101/137 of 6h8kI

query
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6h8kI

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binding iron/sulfur cluster: C42 (= C92), C45 (= C95), C48 (= C98), C52 (= C102), A56 (= A106), C78 (= C123), I79 (≠ T124), Y80 (≠ A125), C81 (= C126), G82 (= G127), Y83 (≠ L128), C84 (= C129), C88 (= C133), A92 (= A137)

7bkdA Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from methanospirillum hungatei (heterodislfide reductase core and mobile arm in conformational state 1, composite structure) (see paper)
40% identity, 36% coverage: 89:141/147 of query aligns to 593:647/664 of 7bkdA

query
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7bkdA

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binding iron/sulfur cluster: C596 (= C92), A597 (≠ M93), C599 (= C95), G600 (= G96), C602 (= C98), C606 (= C102), L611 (= L107), C629 (= C123), K630 (≠ T124), C632 (= C126), G633 (= G127), C635 (= C129), C639 (= C133), I644 (≠ M138)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 149, 150, 152, 153, 173, 174, 180, 181, 185, 188, 189, 339, 340, 341, 342, 368, 419, 422, 552, 557, 559
binding iron/sulfur cluster: 10, 12, 45, 69, 70, 257, 259, 260, 261, 263, 267, 268, 284, 285, 297, 304, 305, 306, 307, 308, 310, 314, 319, 397, 398, 412, 413, 414, 416, 417, 444

8a8oD Paps reductase from methanothermococcus thermolithotrophicus refined to 1.45 a (see paper)
33% identity, 37% coverage: 89:143/147 of query aligns to 6:60/102 of 8a8oD

query
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8a8oD

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binding iron/sulfur cluster: C9 (= C92), I10 (≠ M93), G11 (≠ H94), C12 (= C95), G13 (= G96), C15 (= C98), C19 (= C102), P20 (= P103), S32 (≠ T115), C40 (= C123), W41 (≠ T124), D42 (≠ A125), C43 (= C126), A44 (≠ G127), C46 (= C129), C50 (= C133), I55 (≠ M138)

Sites not aligning to the query:

binding iron/sulfur cluster: 2

7m31A Dihydropyrimidine dehydrogenase (dpd) c671s mutant soaked with thymine and NADPH anaerobically (see paper)
41% identity, 33% coverage: 88:135/147 of query aligns to 943:992/1011 of 7m31A

query
sites
7m31A

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binding iron/sulfur cluster: C947 (= C92), I948 (≠ M93), N949 (≠ H94), C950 (= C95), G951 (= G96), C953 (= C98), C957 (= C102), I965 (≠ L107), P974 (≠ V116), C980 (= C123), T981 (= T124), G982 (≠ A125), C983 (= C126), T984 (≠ G127), C986 (= C129), C990 (= C133), P991 (= P134)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 127, 192, 194, 195, 196, 216, 217, 223, 224, 228, 229, 233, 259, 281, 341, 478, 479, 486, 487, 490
binding flavin mononucleotide: 548, 549, 572, 573, 588, 607, 666, 703, 760, 761, 787, 788, 789, 810, 811, 814
binding nadp nicotinamide-adenine-dinucleotide phosphate: 338, 339, 340, 341, 362, 363, 435, 436, 437, 485
binding iron/sulfur cluster: 77, 78, 79, 80, 84, 85, 89, 93, 95, 128, 133, 134, 138, 139, 140, 148, 150, 154, 940, 995, 996
binding thymine: 607, 610, 730, 731

1h7xA Dihydropyrimidine dehydrogenase (dpd) from pig, ternary complex of a mutant enzyme (c671a), NADPH and 5-fluorouracil (see paper)
41% identity, 33% coverage: 88:135/147 of query aligns to 948:997/1016 of 1h7xA

query
sites
1h7xA

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binding iron/sulfur cluster: C952 (= C92), I953 (≠ M93), N954 (≠ H94), C955 (= C95), G956 (= G96), C958 (= C98), C962 (= C102), I970 (≠ L107), C985 (= C123), T986 (= T124), G987 (≠ A125), C988 (= C126), T989 (≠ G127), C991 (= C129), C995 (= C133), P996 (= P134)

Sites not aligning to the query:

active site: 573, 600, 604, 670, 672, 673, 708, 734
binding flavin-adenine dinucleotide: 128, 193, 195, 196, 197, 217, 218, 224, 225, 234, 259, 260, 282, 283, 285, 342, 480, 487, 488, 491
binding flavin mononucleotide: 549, 550, 573, 574, 589, 608, 667, 708, 765, 766, 792, 793, 794, 815, 816, 819
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 338, 339, 342, 363, 364, 370, 436, 437, 480, 483, 484, 485, 486
binding iron/sulfur cluster: 78, 79, 80, 81, 85, 86, 90, 94, 96, 129, 134, 135, 139, 140, 141, 149, 151, 155, 945, 1001
binding 5-fluorouracil: 608, 610, 735, 736

8f61B Dihydropyrimidine dehydrogenase (dpd) c671s mutant soaked with dihydrothymine quasi-anaerobically (see paper)
41% identity, 33% coverage: 88:135/147 of query aligns to 947:996/1016 of 8f61B

query
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8f61B

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binding iron/sulfur cluster: C951 (= C92), I952 (≠ M93), N953 (≠ H94), C954 (= C95), G955 (= G96), C957 (= C98), C961 (= C102), Y966 (vs. gap), I969 (≠ L107), P978 (≠ V116), C984 (= C123), T985 (= T124), G986 (≠ A125), C987 (= C126), T988 (≠ G127), C990 (= C129), C994 (= C133), P995 (= P134)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 127, 192, 194, 195, 196, 216, 217, 223, 224, 229, 233, 258, 259, 281, 341, 344, 478, 479, 486, 487, 490
binding flavin mononucleotide: 548, 549, 572, 573, 607, 666, 707, 734, 764, 765, 791, 792, 793, 814, 815
binding iron/sulfur cluster: 77, 78, 79, 80, 84, 85, 89, 93, 95, 128, 133, 134, 138, 139, 140, 148, 150, 154, 488, 944, 999, 1000
binding (5S)-5-methyl-1,3-diazinane-2,4-dione: 607, 609, 734, 735

7m32A Dihydropyrimidine dehydrogenase (dpd) c671a mutant soaked with uracil and NADPH anaerobically (see paper)
41% identity, 33% coverage: 88:135/147 of query aligns to 941:990/1011 of 7m32A

query
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7m32A

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binding iron/sulfur cluster: C945 (= C92), I946 (≠ M93), N947 (≠ H94), C948 (= C95), G949 (= G96), C951 (= C98), C955 (= C102), I963 (≠ L107), P972 (≠ V116), C978 (= C123), T979 (= T124), G980 (≠ A125), C981 (= C126), T982 (≠ G127), C984 (= C129), C988 (= C133), P989 (= P134)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 127, 192, 194, 195, 196, 216, 217, 223, 224, 233, 258, 259, 281, 282, 340, 341, 478, 479, 486, 487, 490
binding 1-deoxy-1-(7,8-dimethyl-2,4-dioxo-3,4-dihydro-2h-benzo[g]pteridin-1-id-10(5h)-yl)-5-o-phosphonato-d-ribitol: 548, 549, 572, 573, 588, 607, 666, 701, 758, 759, 786, 787, 808, 809, 812
binding leucine: 577, 615
binding iron/sulfur cluster: 77, 78, 79, 80, 84, 85, 89, 95, 128, 133, 134, 138, 139, 140, 148, 150, 154, 938, 994
binding uracil: 607, 609, 728, 729

7ljsA Porcine dihydropyrimidine dehydrogenase (dpd) complexed with 5- ethynyluracil (5eu) - open form (see paper)
41% identity, 33% coverage: 88:135/147 of query aligns to 937:986/1005 of 7ljsA

query
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7ljsA

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binding iron/sulfur cluster: C941 (= C92), I942 (≠ M93), C944 (= C95), G945 (= G96), C947 (= C98), C951 (= C102), I959 (≠ L107), P968 (≠ V116), C974 (= C123), T975 (= T124), G976 (≠ A125), C977 (= C126), T978 (≠ G127), C980 (= C129), C984 (= C133), P985 (= P134)

Sites not aligning to the query:

active site: 573, 670, 703
binding flavin-adenine dinucleotide: 128, 193, 195, 196, 197, 217, 218, 224, 225, 230, 234, 260, 282, 341, 342, 479, 480, 486, 487, 488, 491
binding flavin mononucleotide: 549, 550, 573, 574, 608, 667, 703, 760, 761, 787, 788, 789, 810, 811
binding iron/sulfur cluster: 78, 80, 81, 86, 90, 94, 96, 129, 135, 139, 140, 141, 149, 155, 989
binding 5-ethynylpyrimidine-2,4(1H,3H)-dione: 608, 611, 612, 730, 731

1gteA Dihydropyrimidine dehydrogenase (dpd) from pig, binary complex with 5- iodouracil (see paper)
41% identity, 33% coverage: 88:135/147 of query aligns to 937:986/1005 of 1gteA

query
sites
1gteA

D

D

E

E

G

M

C
|
C

M
x
I

H
x
N

C
|
C

G
|
G

M
x
K

C
|
C

T

Y

A

M

I

T

C
|
C

P

N

T

D

S

S

-

G

-

Y

-

Q

A

A

L
x
I

R

Q

M

F

N

D

L

P

E

E

N

T

R

H

T

L

V

P

T

T

F
x
V

E

T

L

-

D

D

R

T

C
|
C

T
|
T

A
x
G

C
|
C

G
x
T

L

L

C
|
C

T

L

R

S

V

V

C
|
C

P
|
P

V

I

binding iron/sulfur cluster: C941 (= C92), I942 (≠ M93), N943 (≠ H94), C944 (= C95), G945 (= G96), K946 (≠ M97), C947 (= C98), C951 (= C102), I959 (≠ L107), V970 (≠ F118), C974 (= C123), T975 (= T124), G976 (≠ A125), C977 (= C126), T978 (≠ G127), C980 (= C129), C984 (= C133), P985 (= P134)

Sites not aligning to the query:

active site: 573, 600, 604, 670, 672, 703, 729
binding flavin-adenine dinucleotide: 128, 193, 195, 196, 197, 217, 218, 224, 225, 234, 259, 260, 282, 283, 285, 341, 342, 479, 480, 486, 487, 488, 491
binding flavin mononucleotide: 549, 550, 573, 574, 589, 608, 667, 703, 730, 760, 761, 787, 788, 789, 810, 811, 814
binding 5-iodouracil: 608, 611, 612, 667, 730, 731
binding iron/sulfur cluster: 78, 79, 80, 81, 85, 86, 90, 94, 96, 129, 131, 134, 135, 139, 140, 141, 149, 151, 155, 934, 936, 990

Query Sequence

>207401 MicrobesOnline__882:207401
MSAQPQKPYRKNVHLTFPPEISGNPLVCNLTRLFDLTFNILKAQITPRKEGYLTLELMGD
EANCTKAMEYLREHDVVVTPVAQRISRDEEGCMHCGMCTAICPTSALRMNLENRTVTFEL
DRCTACGLCTRVCPVAAMHVELENGHY

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory