SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 207406 MicrobesOnline__882:207406 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
42% identity, 82% coverage: 33:249/265 of query aligns to 17:223/241 of 4u00A

query
sites
4u00A

V
|
V

L

L

K

K

D

G

I

I

S

H

F

L

T

E

V

V

S

A

G

P

Q

G

S

E

T

K

T

L

A

V

I

I

I

I

G

G

P

P

S
|
S

G
|
G

T
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

C

T

I

I

N

N

R

R

L

L

I

E

E

D

P

F

T

Q

A

E

G

G

R

E

I

V

L

V

V

V

S

D

G

G

E

L

D

S

V

V

C

K

A

-

L

-

K

D

G

D

T

R

A

A

L

L

R

R

E

E

A

I

R

R

R

R

R

E

I

V

G

G

M

M

V

V

F

F

Q

Q

E

Q

Y

F

N

N

L

L

V

F

E

P

R

H

L

M

S

T

V

V

M

L

E

E

N

N

V

V

L

-

C

-

G

-

R

-

L

-

G

-

Y

T

I

L

S

A

P

P

W

M

R

R

A

-

W

-

L

V

R

R

K

R

F

W

P

P

Q

R

E

E

D

K

I

A

D

E

R

K

-

K

A

A

F

L

D

E

L

L

L

L

D

E

M

R

V

V

G

G

L

I

A

L

D

D

F

Q

A

A

R

R

A

K

R

Y

A

P

D

A

E

Q

L

L

S

S

G

G

G

G

Q

Q

R

Q

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

V

L

M

A

Q

M

E

E

P

P

H

K

I

I

L

M

L

L

A

F

D

D

E

E

P

P

T

T

S

S

S

A

L

L

D

D

P

P

K

E

T

M

S

V

V

G

E

E

I

V

M

L

E

D

L

V

L

M

R

R

A

D

V

L

A

A

E

Q

K

G

R

-

D

G

I

M

P

T

V

M

L

V

V

V

N

V

I

T

H

H

D

E

V

M

T

G

L

F

G

A

R

R

R

E

F

V

S

A

D

D

R

R

V

V

I

V

G

F

M

M

C

D

K

G

G

G

E

Q

V

I

L

V

F

E

D

E

D

G

V

R

P

P

S

E

A

E

L

I

binding adenosine-5'-diphosphate: V17 (= V33), S37 (= S53), G38 (= G54), S39 (≠ T55), G40 (= G56), K41 (= K57), S42 (= S58), T43 (= T59)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

3c4jA Abc protein artp in complex with atp-gamma-s
39% identity, 87% coverage: 22:251/265 of query aligns to 7:227/242 of 3c4jA

query
sites
3c4jA

H

H

L

Q

R

L

K

K

E

K

Y

S

V
x
F

R

G

G

S

K

L

A

E

V
|
V

L

L

K

K

D

G

I

I

S

N

F

V

T

H

V

I

S

R

G

E

Q

G

S

E

T

V

T

V

A

V

I

V

I

I

G

G

P

P

S
|
S

G
|
G

T
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

C

C

I

L

N

N

R

L

L

L

I

E

E

D

P

F

T

D

A

E

G

G

R

E

I

I

L

I

V

I

S

D

G

G

E

I

D

N

V

L

C

K

A

A

L

-

K

K

G

D

T

T

A

N

L

L

R

N

E

K

A

V

R

R

R

E

R

E

I

V

G

G

M

M

V

V

F

F

Q

Q

E

R

Y

F

N

N

L

L

V

F

E

P

R

H

L

M

S

T

V

V

M

L

E

N

N

N

V

I

L

-

C

-

G

-

R

-

L

-

G

-

Y

T

I

L

S

A

P

P

W

M

R

K

A

-

W

-

L

V

R

R

K

K

F

W

P

P

Q

R

E

E

D

K

I

A

D

E

-

A

R

K

A

A

F

M

D

E

L

L

L

L

D

D

M

K

V

V

G

G

L

L

A

K

D

D

F

K

A

A

R

H

A

A

R

Y

A

P

D

D

E

S

L

L

S

S

G

G

G

G

Q

Q

R

A

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

V

L

M

A

Q

M

E

E

P

P

H

K

I

I

L

M

L

L

A

F

D

D

E

E

P

P

T

T

S

S

S

A

L

L

D

D

P

P

K

E

T

M

S

V

V

G

E

E

I

V

M

L

E

S

L

V

L

M

R

K

A

Q

V

L

A

A

E

N

K

E

R

-

D

G

I

M

P

T

V

M

L

V

V

V

N

V

I

T

H

H

D

E

V

M

T

G

L

F

G

A

R

R

R

E

F

V

S

G

D

D

R

R

V

V

I

L

G

F

M

M

C

D

K

G

G

G

E

Y

V

I

L

I

F

E

D

E

D

G

V

K

P

P

S

E

A

D

L

L

Q

F

D

D

binding phosphothiophosphoric acid-adenylate ester: F13 (≠ V28), V18 (= V33), S38 (= S53), G39 (= G54), S40 (≠ T55), G41 (= G56), K42 (= K57), S43 (= S58), T44 (= T59)

3c41J Abc protein artp in complex with amp-pnp/mg2+
39% identity, 87% coverage: 22:251/265 of query aligns to 7:227/242 of 3c41J

query
sites
3c41J

H

H

L

Q

R

L

K

K

E

K

Y

S

V
x
F

R

G

G

S

K

L

A

E

V
|
V

L

L

K

K

D

G

I

I

S

N

F

V

T

H

V

I

S

R

G

E

Q

G

S

E

T

V

T

V

A

V

I

V

I

I

G

G

P

P

S
|
S

G
|
G

T

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

C

C

I

L

N

N

R

L

L

L

I

E

E

D

P

F

T

D

A

E

G

G

R

E

I

I

L

I

V

I

S

D

G

G

E

I

D

N

V

L

C

K

A

A

L

-

K

K

G

D

T

T

A

N

L

L

R

N

E

K

A

V

R

R

R

E

R

E

I

V

G

G

M

M

V

V

F

F

Q

Q

E

R

Y

F

N

N

L

L

V

F

E

P

R

H

L

M

S

T

V

V

M

L

E

N

N

N

V

I

L

-

C

-

G

-

R

-

L

-

G

-

Y

T

I

L

S

A

P

P

W

M

R

K

A

-

W

-

L

V

R

R

K

K

F

W

P

P

Q

R

E

E

D

K

I

A

D

E

-

A

R

K

A

A

F

M

D

E

L

L

L

L

D

D

M

K

V

V

G

G

L

L

A

K

D

D

F

K

A

A

R

H

A

A

R

Y

A

P

D

D

E

S

L

L

S

S

G

G

G

G

Q

Q

R

A

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

V

L

M

A

Q

M

E

E

P

P

H

K

I

I

L

M

L

L

A

F

D
|
D

E

E

P

P

T

T

S

S

S

A

L

L

D

D

P

P

K

E

T

M

S

V

V

G

E

E

I

V

M

L

E

S

L

V

L

M

R

K

A

Q

V

L

A

A

E

N

K

E

R

-

D

G

I

M

P

T

V

M

L

V

V

V

N

V

I

T

H

H

D

E

V

M

T

G

L

F

G

A

R

R

R

E

F

V

S

G

D

D

R

R

V

V

I

L

G

F

M

M

C

D

K

G

G

G

E

Y

V

I

L

I

F

E

D

E

D

G

V

K

P

P

S

E

A

D

L

L

Q

F

D

D

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ V28), V18 (= V33), S38 (= S53), G39 (= G54), G41 (= G56), K42 (= K57), S43 (= S58), T44 (= T59)
binding magnesium ion: S43 (= S58), D163 (= D186)

2olkA Abc protein artp in complex with adp-beta-s
39% identity, 87% coverage: 22:251/265 of query aligns to 7:227/242 of 2olkA

query
sites
2olkA

H

H

L

Q

R

L

K

K

E

K

Y

S

V
x
F

R

G

G

S

K

L

A

E

V
|
V

L

L

K

K

D

G

I

I

S

N

F

V

T

H

V

I

S

R

G

E

Q

G

S

E

T

V

T

V

A

V

I

V

I

I

G

G

P

P

S
|
S

G
|
G

T
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

C

C

I

L

N

N

R

L

L

L

I

E

E

D

P

F

T

D

A

E

G

G

R

E

I

I

L

I

V

I

S

D

G

G

E

I

D

N

V

L

C

K

A

A

L

-

K

K

G

D

T

T

A

N

L

L

R

N

E

K

A

V

R

R

R

E

R

E

I

V

G

G

M

M

V

V

F

F

Q

Q

E

R

Y

F

N

N

L

L

V

F

E

P

R

H

L

M

S

T

V

V

M

L

E

N

N

N

V

I

L

-

C

-

G

-

R

-

L

-

G

-

Y

T

I

L

S

A

P

P

W

M

R

K

A

-

W

-

L

V

R

R

K

K

F

W

P

P

Q

R

E

E

D

K

I

A

D

E

-

A

R

K

A

A

F

M

D

E

L

L

L

L

D

D

M

K

V

V

G

G

L

L

A

K

D

D

F

K

A

A

R

H

A

A

R

Y

A

P

D

D

E

S

L

L

S

S

G

G

G

G

Q

Q

R

A

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

V

L

M

A

Q

M

E

E

P

P

H

K

I

I

L

M

L

L

A

F

D

D

E

E

P

P

T

T

S

S

S

A

L

L

D

D

P

P

K

E

T

M

S

V

V

G

E

E

I

V

M

L

E

S

L

V

L

M

R

K

A

Q

V

L

A

A

E

N

K

E

R

-

D

G

I

M

P

T

V

M

L

V

V

V

N

V

I

T

H

H

D

E

V

M

T

G

L

F

G

A

R

R

R

E

F

V

S

G

D

D

R

R

V

V

I

L

G

F

M

M

C

D

K

G

G

G

E

Y

V

I

L

I

F

E

D

E

D

G

V

K

P

P

S

E

A

D

L

L

Q

F

D

D

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ V28), V18 (= V33), S38 (= S53), G39 (= G54), S40 (≠ T55), G41 (= G56), K42 (= K57), S43 (= S58), T44 (= T59)

2oljA Abc protein artp in complex with adp/mg2+
39% identity, 87% coverage: 22:251/265 of query aligns to 7:227/242 of 2oljA

query
sites
2oljA

H

H

L

Q

R

L

K

K

E

K

Y

S

V
x
F

R

G

G

S

K

L

A

E

V
|
V

L

L

K

K

D

G

I

I

S

N

F

V

T

H

V

I

S

R

G

E

Q

G

S

E

T

V

T

V

A

V

I

V

I

I

G

G

P

P

S
|
S

G
|
G

T
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

C

C

I

L

N

N

R

L

L

L

I

E

E

D

P

F

T

D

A

E

G

G

R

E

I

I

L

I

V

I

S

D

G

G

E

I

D

N

V

L

C

K

A

A

L

-

K

K

G

D

T

T

A

N

L

L

R

N

E

K

A

V

R

R

R

E

R

E

I

V

G

G

M

M

V

V

F

F

Q

Q

E

R

Y

F

N

N

L

L

V

F

E

P

R

H

L

M

S

T

V

V

M

L

E

N

N

N

V

I

L

-

C

-

G

-

R

-

L

-

G

-

Y

T

I

L

S

A

P

P

W

M

R

K

A

-

W

-

L

V

R

R

K

K

F

W

P

P

Q

R

E

E

D

K

I

A

D

E

-

A

R

K

A

A

F

M

D

E

L

L

L

L

D

D

M

K

V

V

G

G

L

L

A

K

D

D

F

K

A

A

R

H

A

A

R

Y

A

P

D

D

E

S

L

L

S

S

G

G

G

G

Q

Q

R

A

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

V

L

M

A

Q

M

E

E

P

P

H

K

I

I

L

M

L

L

A

F

D

D

E

E

P

P

T

T

S

S

S

A

L

L

D

D

P

P

K

E

T

M

S

V

V

G

E

E

I

V

M

L

E

S

L

V

L

M

R

K

A

Q

V

L

A

A

E

N

K

E

R

-

D

G

I

M

P

T

V

M

L

V

V

V

N

V

I

T

H

H

D

E

V

M

T

G

L

F

G

A

R

R

R

E

F

V

S

G

D

D

R

R

V

V

I

L

G

F

M

M

C

D

K

G

G

G

E

Y

V

I

L

I

F

E

D

E

D

G

V

K

P

P

S

E

A

D

L

L

Q

F

D

D

binding adenosine-5'-diphosphate: F13 (≠ V28), V18 (= V33), S38 (= S53), G39 (= G54), S40 (≠ T55), G41 (= G56), K42 (= K57), S43 (= S58), T44 (= T59)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
38% identity, 83% coverage: 29:247/265 of query aligns to 16:226/343 of P30750

query
sites
P30750

R

R

G

T

K

I

A

Q

V

A

L

L

K

N

D

N

I

V

S

S

F

L

T

H

V

V

S

P

G

A

Q

G

S

Q

T

I

T

Y

A

G

I

V

I

I

G

G

P

A

S
|
S

G
|
G

T
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

C

C

I

V

N

N

R

L

L

L

I

E

E

R

P

P

T

T

A

E

G

G

R

S

I

V

L

L

V

V

S

D

G

G

E

Q

D

E

V

L

C

T

A

T

L

L

K

S

G

E

T

S

A

E

L

L

R

T

E

K

A

A

R

R

R

R

R

Q

I

I

G

G

M

M

V

I

F

F

Q

Q

E

H

Y

F

N

N

L

L

V

L

E

S

R

S

L

R

S

T

V

V

M

F

E

G

N

N

V

V

L

A

C

L

G

-

R

-

L

-

G

-

Y

-

I

-

S

-

P

P

W

L

R

E

A

-

W

-

L

L

R

D

K

N

F

T

P

P

Q

K

E

D

D

E

I

V

D

K

R

R

-

R

A

V

F

T

D

E

L

L

L

L

D

S

M

L

V

V

G

G

L

L

A

G

D

D

F

K

A

H

R

D

A

S

R

Y

A

P

D

S

E

N

L

L

S

S

G

G

G

G

Q

Q

R

K

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

V

L

M

A

Q

S

E

N

P

P

H

K

I

V

L

L

L

L

A

C

D

D

E
|
E

P

A

T

T

S

S

S

A

L

L

D

D

P

P

K

A

T

T

S

T

V

R

E

S

I

I

M

L

E

E

L

L

L

L

R

K

A

D

V

I

A

N

E

R

K

R

R

L

D

G

I

L

P

T

V

I

L

L

V

L

N

I

I

T

H

H

D

E

V

M

T

D

L

V

G

V

R

K

R

R

F

I

S

C

D

D

R

C

V

V

I

A

G

V

M

I

C

S

K

N

G

G

E

E

V

L

L

I

F

E

D

Q

D

D

V

T

P

V

S

S

40:46 (vs. 53:59, 86% identical) binding
E166 (= E187) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
38% identity, 83% coverage: 29:247/265 of query aligns to 17:227/344 of 3tuzC

query
sites
3tuzC

R

R

G

T

K

I

A

Q

V

A

L

L

K

N

D

N

I

V

S

S

F

L

T

H

V

V

S

P

G

A

Q

G

S

Q

T

I

T

Y

A

G

I

V

I

I

G

G

P

A

S

S

G
|
G

T

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

C

C

I

V

N

N

R

L

L

L

I

E

E

R

P

P

T

T

A

E

G

G

R

S

I

V

L

L

V

V

S

D

G

G

E

Q

D

E

V

L

C

T

A

T

L

L

K

S

G

E

T

S

A

E

L

L

R

T

E

K

A

A

R

R

R

R

R

Q

I

I

G

G

M

M

V

I

F

F

Q

Q

E

H

Y

F

N

N

L

L

V

L

E

S

R

S

L

R

S

T

V

V

M

F

E

G

N

N

V

V

L

A

C

L

G

-

R

-

L

-

G

-

Y

-

I

-

S

-

P

P

W

L

R

E

A

-

W

-

L

L

R

D

K

N

F

T

P

P

Q

K

E

D

D

E

I

V

D

K

R

R

-

R

A

V

F

T

D

E

L

L

L

L

D

S

M

L

V

V

G

G

L

L

A

G

D

D

F

K

A

H

R

D

A

S

R

Y

A

P

D

S

E

N

L

L

S

S

G

G

G

G

Q

Q

R

K

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

V

L

M

A

Q

S

E

N

P

P

H

K

I

V

L

L

L

L

A

C

D

D

E

Q

P

A

T

T

S

S

S

A

L

L

D

D

P

P

K

A

T

T

S

T

V

R

E

S

I

I

M

L

E

E

L

L

L

L

R

K

A

D

V

I

A

N

E

R

K

R

R

L

D

G

I

L

P

T

V

I

L

L

V

L

N

I

I

T

H

H

D

E

V

M

T

D

L

V

G

V

R

K

R

R

F

I

S

C

D

D

R

C

V

V

I

A

G

V

M

I

C

S

K

N

G

G

E

E

V

L

L

I

F

E

D

Q

D

D

V

T

P

V

S

S

binding adenosine-5'-diphosphate: G42 (= G54), G44 (= G56), K45 (= K57), S46 (= S58), T47 (= T59)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 14
binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
38% identity, 83% coverage: 29:247/265 of query aligns to 17:227/344 of 3tuiC

query
sites
3tuiC

R

R

G

T

K

I

A

Q

V

A

L

L

K

N

D

N

I

V

S

S

F

L

T

H

V

V

S

P

G

A

Q

G

S

Q

T

I

T

Y

A

G

I

V

I

I

G

G

P

A

S

S

G
|
G

T
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

C

C

I

V

N

N

R

L

L

L

I

E

E

R

P

P

T

T

A

E

G

G

R

S

I

V

L

L

V

V

S

D

G

G

E

Q

D

E

V

L

C

T

A

T

L

L

K

S

G

E

T

S

A

E

L

L

R

T

E

K

A

A

R

R

R

R

R

Q

I

I

G

G

M

M

V

I

F

F

Q

Q

E

H

Y

F

N

N

L

L

V

L

E

S

R

S

L

R

S

T

V

V

M

F

E

G

N

N

V

V

L

A

C

L

G

-

R

-

L

-

G

-

Y

-

I

-

S

-

P

P

W

L

R

E

A

-

W

-

L

L

R

D

K

N

F

T

P

P

Q

K

E

D

D

E

I

V

D

K

R

R

-

R

A

V

F

T

D

E

L

L

L

L

D

S

M

L

V

V

G

G

L

L

A

G

D

D

F

K

A

H

R

D

A

S

R

Y

A

P

D

S

E

N

L

L

S

S

G

G

G

G

Q

Q

R

K

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

V

L

M

A

Q

S

E

N

P

P

H

K

I

V

L

L

L

L

A

C

D

D

E

Q

P

A

T

T

S

S

S

A

L

L

D

D

P

P

K

A

T

T

S

T

V

R

E

S

I

I

M

L

E

E

L

L

L

L

R

K

A

D

V

I

A

N

E

R

K

R

R

L

D

G

I

L

P

T

V

I

L

L

V

L

N

I

I

T

H

H

D

E

V

M

T

D

L

V

G

V

R

K

R

R

F

I

S

C

D

D

R

C

V

V

I

A

G

V

M

I

C

S

K

N

G

G

E

E

V

L

L

I

F

E

D

Q

D

D

V

T

P

V

S

S

binding adenosine-5'-diphosphate: G42 (= G54), A43 (≠ T55), G44 (= G56), K45 (= K57), S46 (= S58), T47 (= T59)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
38% identity, 83% coverage: 29:247/265 of query aligns to 17:227/344 of 6cvlD

query
sites
6cvlD

R

R

G

T

K
x
I

A

Q

V

A

L

L

K

N

D

N

I

V

S

S

F

L

T

H

V

V

S

P

G

A

Q

G

S

Q

T

I

T

Y

A

G

I

V

I

I

G

G

P

A

S
|
S

G
|
G

T
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

C

C

I

V

N

N

R

L

L

L

I

E

E

R

P

P

T

T

A

E

G

G

R

S

I

V

L

L

V

V

S

D

G

G

E

Q

D

E

V

L

C

T

A

T

L

L

K

S

G

E

T

S

A

E

L

L

R

T

E

K

A

A

R

R

R

R

R

Q

I

I

G

G

M

M

V

I

F

F

Q

Q

E

H

Y

F

N

N

L

L

V

L

E

S

R

S

L

R

S

T

V

V

M

F

E

G

N

N

V

V

L

A

C

L

G

-

R

-

L

-

G

-

Y

-

I

-

S

-

P

P

W

L

R

E

A

-

W

-

L

L

R

D

K

N

F

T

P

P

Q

K

E

D

D

E

I

V

D

K

R

R

-

R

A

V

F

T

D

E

L

L

L

L

D

S

M

L

V

V

G

G

L

L

A

G

D

D

F

K

A

H

R

D

A

S

R

Y

A

P

D

S

E
x
N

L

L

S
|
S

G

G

G

G

Q
|
Q

R

K

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

V

L

M

A

Q

S

E

N

P

P

H

K

I

V

L

L

L

L

A

C

D

D

E

Q

P

A

T

T

S

S

S

A

L

L

D

D

P

P

K

A

T

T

S

T

V

R

E

S

I

I

M

L

E

E

L

L

L

L

R

K

A

D

V

I

A

N

E

R

K

R

R

L

D

G

I

L

P

T

V

I

L

L

V

L

N

I

I

T

H
|
H

D

E

V

M

T

D

L

V

G

V

R

K

R

R

F

I

S

C

D

D

R

C

V

V

I

A

G

V

M

I

C

S

K

N

G

G

E

E

V

L

L

I

F

E

D

Q

D

D

V

T

P

V

S

S

binding phosphothiophosphoric acid-adenylate ester: I19 (≠ K31), S41 (= S53), G42 (= G54), A43 (≠ T55), G44 (= G56), K45 (= K57), S46 (= S58), T47 (= T59), N141 (≠ E161), S143 (= S163), Q146 (= Q166), H200 (= H220)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12, 14

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
37% identity, 82% coverage: 33:249/265 of query aligns to 16:223/240 of 4ymuJ

query
sites
4ymuJ

V
|
V

L

L

K

K

D

G

I

V

S

T

F

L

T

K

V

V

S

N

G

K

Q

G

S

E

T

V

T

V

A

V

I

I

I

I

G

G

P

P

S
|
S

G
|
G

T
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

L

R

R

C

C

I

I

N

N

R

L

L

L

I

E

E

E

P

P

T

T

A

K

G

G

R

E

I

V

L

F

V

I

S

D

G

G

E

V

D

K

V

I

C

N

A

N

L

G

K

K

G

-

T

V

A

N

L

I

R

N

E

K

A

V

R

R

R

Q

R

K

I

V

G

G

M

M

V

V

F

F

Q

Q

E

H

Y

F

N

N

L

L

V

F

E

P

R

H

L

L

S

T

V

A

M

I

E

E

N

N

V

I

L

-

C

-

G

-

R

-

L

-

G

-

Y

T

I

L

S

A

P

P

W

V

R

K

A

-

W

-

L

V

R

K

K

K

F

M

P

N

Q

K

E

K

D

E

I

A

D

E

R

E

-

L

A

A

F

V

D

D

L

L

L

L

D

A

M

K

V

V

G

G

L

L

A

L

D

D

F

K

A

K

R

D

A

Q

R

Y

A

P

D

I

E

K

L

L

S

S

G

G

G

G

Q

Q

R

K

Q

Q

R

R

V

L

G

A

I

I

A

A

R

R

A

A

V

L

M

A

Q

M

E

Q

P

P

H

E

I

V

L

M

L

L

A

F

D

D

E
|
E

P

P

T

T

S

S

S

A

L

L

D

D

P

P

K

E

T

M

S

V

V

K

E

E

I

V

M

L

E

N

L

V

L

M

R

K

A

Q

V

L

A

A

E

N

K

E

R

-

D

G

I

M

P

T

V

M

L

V

V

V

N

V

I

T

H
|
H

D

E

V

M

T

G

L

F

G

A

R

R

R

E

F

V

S

G

D

D

R

R

V

V

I

I

G

F

M

M

C

D

K

D

G

G

E

V

V

I

L

V

F

E

D

E

D

G

V

T

P

P

S

E

A

E

L

I

binding adenosine-5'-triphosphate: V16 (= V33), S36 (= S53), G37 (= G54), S38 (≠ T55), G39 (= G56), K40 (= K57), S41 (= S58), T42 (= T59), E162 (= E187), H194 (= H220)
binding magnesium ion: S41 (= S58), E162 (= E187)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 11

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
38% identity, 83% coverage: 20:240/265 of query aligns to 4:223/232 of 1f3oA

query
sites
1f3oA

V

L

E

K

H

N

L

V

R

T

K

K

E

T

Y
|
Y

V

K

R

M

G

G

K

E

A

E

V

I

-

I

-

Y

-

A

L

L

K

K

D

N

I

V

S

N

F

L

T

N

V

I

S

K

G

E

Q

G

S

E

T

F

T

V

A

S

I

I

I

M

G

G

P

P

S

S

G
|
G

T

S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

R

N

C

I

I

I

N

G

R

C

L

L

I

D

E

K

P

P

T

T

A

E

G

G

R

E

I

V

L

Y

V

I

S

D

G

N

E

I

D

K

V

T

C

N

A

D

L

L

K

D

G

D

T

D

A

E

L

L

R

T

E

K

A

I

R

R

R

R

-

D

R

K

I

I

G

G

M

F

V

V

F

F

Q

Q

E

Q

Y

F

N

N

L

L

V

I

E

P

R

L

L

L

S

T

V

A

M

L

E

E

N

N

V

V

L

-

C

-

G

-

R

E

L

L

G

P

Y

L

I

I

S

F

P

K

W

Y

R

R

A

G

W

A

L

M

R

S

K

G

F

-

P

-

Q

E

E

E

D

R

I

R

D

K

R

R

A

A

F

L

D

E

L

C

L

L

D

K

M

M

V

A

G

E

L

L

A

E

D

E

-

R

F

F

A

A

R

N

A

H

R

K

A

P

D

N

E

Q

L

L

S

S

G

G

G

G

Q

Q

R

Q

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

V

L

M

A

Q

N

E

N

P

P

H

P

I

I

L

I

L

L

A

A

D

D

E

E

P

P

T

T

S

G

S

A

L

L

D

D

P

S

K

K

T

T

S

G

V

E

E

K

I

I

M

M

E

Q

L

L

L

L

R

K

A

K

V

L

A

N

E

E

K

E

R

D

D

G

I

K

P

T

V

V

L

V

V

V

N

V

I

T

H

H

D

D

V

I

T

N

L

V

G

A

R

-

R

R

F

F

S

G

D

E

R

R

V

I

I

I

G

Y

M

L

C

K

K

D

G

G

E

E

V

V

binding adenosine-5'-diphosphate: Y11 (= Y27), G41 (= G54), G43 (= G56), K44 (= K57), S45 (= S58), T46 (= T59)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
38% identity, 83% coverage: 20:240/265 of query aligns to 4:223/230 of 1l2tA

query
sites
1l2tA

V

L

E

K

H

N

L

V

R

T

K

K

E

T

Y
|
Y

V

K

R

M

G

G

K

E

A

E

V

I

-

I

-

Y

-

A

L

L

K

K

D

N

I

V

S

N

F

L

T

N

V

I

S

K

G

E

Q

G

S

E

T

F

T

V

A

S

I

I

I

M

G

G

P

P

S
|
S

G
|
G

T
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

R

N

C

I

I

I

N

G

R

C

L

L

I

D

E

K

P

P

T

T

A

E

G

G

R

E

I

V

L

Y

V

I

S

D

G

N

E

I

D

K

V

T

C

N

A

D

L

L

K

D

G

D

T

D

A

E

L

L

R

T

E

K

A

I

R

R

R

R

-

D

R

K

I

I

G

G

M

F

V

V

F

F

Q

Q

E

Q

Y

F

N

N

L

L

V

I

E

P

R

L

L

L

S

T

V

A

M

L

E

E

N

N

V

V

L

-

C

-

G

-

R

E

L

L

G

P

Y

L

I

I

S

F

P

K

W

Y

R

R

A

G

W

A

L

M

R

S

K

G

F

-

P

-

Q

E

E

E

D

R

I

R

D

K

R

R

A

A

F

L

D

E

L

C

L

L

D

K

M

M

V

A

G

E

L

L

A

E

D

E

-

R

F
|
F

A

A

R

N

A

H

R

K

A

P

D

N

E
x
Q

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

Q

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

V

L

M

A

Q

N

E

N

P

P

H

P

I

I

L

I

L

L

A

A

D

D

E

Q

P

P

T

T

S

G

S

A

L

L

D

D

P

S

K

K

T

T

S

G

V

E

E

K

I

I

M

M

E

Q

L

L

L

L

R

K

A

K

V

L

A

N

E

E

K

E

R

D

D

G

I

K

P

T

V

V

L

V

V

V

N

V

I

T

H
|
H

D

D

V

I

T

N

L

V

G

A

R

-

R

R

F

F

S

G

D

E

R

R

V

I

I

I

G

Y

M

L

C

K

K

D

G

G

E

E

V

V

binding adenosine-5'-triphosphate: Y11 (= Y27), S40 (= S53), G41 (= G54), S42 (≠ T55), G43 (= G56), K44 (= K57), S45 (= S58), T46 (= T59), F138 (= F154), Q145 (≠ E161), S147 (= S163), G149 (= G165), Q150 (= Q166), H204 (= H220)

6z4wA Ftse structure from streptococcus pneumoniae in complex with adp (space group p 1) (see paper)
35% identity, 83% coverage: 25:244/265 of query aligns to 11:221/230 of 6z4wA

query
sites
6z4wA

K

K

E

K

Y
|
Y

V

D

R

N

G

G

K
x
T

A

T

V
x
A

L

L

K

R

D

G

I

V

S

S

F

V

T

S

V

V

S

Q

G

P

Q

G

S

E

T

F

T

A

A

Y

I

I

I

V

G

G

P

P

S

S

G
|
G

T

A

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

I

R

R

C

S

I

L

N

Y

R

R

L

E

I

V

E

K

P

I

T

D

A

K

G

G

R

S

I

L

L

S

V

V

S

A

G

G

E

F

D

N

V

L

C

V

A

K

L

I

K

K

G

K

T

K

A

D

L

V

R

P

E

L

A

L

R

R

R

R

R

S

I

V

G

G

M

V

V

V

F

F

Q

Q

E

D

Y

Y

N

K

L

L

V

L

E

P

R

K

L

K

S

T

V

V

M

Y

E

E

N

N

V

I

L

A

C

Y

G

A

R

-

L

M

G

E

Y

V

I

I

S

G

P

E

W

N

R

R

A

R

W

N

L

I

R

K

K

R

F

-

P

-

Q

-

E

-

D

-

I

-

D

-

R

R

A

V

F

M

D

E

L

V

L

L

D

D

M

L

V

V

G

G

L

L

A

K

D

H

F

K

A

V

R

R

A

S

R

F

A

P

D

N

E

E

L

L

S

S

G

G

G

G

Q

E

R

Q

Q

Q

R

R

V

I

G

A

I

I

A

A

R

R

A

A

V

I

M

V

Q

N

E

N

P

P

H

K

I

V

L

L

L

I

A

A

D

D

E

E

P

P

T

T

S

G

S

N

L

L

D

D

P

P

K

D

T

N

S

S

V

W

E

E

I

I

M

M

E

N

L

L

L

L

R

E

A

R

V

I

A

-

E

N

K

L

R

Q

D

G

I

T

P

T

V

I

L

L

V

M

N

A

I

T

H

H

D

N

V

S

T

Q

L

I

G

V

R

N

R

T

F

L

S

R

D

H

R

R

V

V

I

I

G

A

M

I

C

E

K

N

G

G

E

R

V

V

L

V

F

R

D

D

D

E

binding adenosine-5'-diphosphate: Y13 (= Y27), T17 (≠ K31), A19 (≠ V33), G40 (= G54), G42 (= G56), K43 (= K57), S44 (= S58), T45 (= T59)

6z67B Ftse structure of streptococcus pneumoniae in complex with amppnp at 2.4 a resolution (see paper)
35% identity, 83% coverage: 25:244/265 of query aligns to 11:221/229 of 6z67B

query
sites
6z67B

K

K

E

K

Y
|
Y

V

D

R

N

G
|
G

K

T

A

T

V
x
A

L

L

K

R

D

G

I

V

S

S

F

V

T

S

V

V

S

Q

G

P

Q

G

S

E

T

F

T

A

A

Y

I

I

I

V

G

G

P

P

S
|
S

G
|
G

T

A

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

I

R

R

C

S

I

L

N

Y

R

R

L

E

I

V

E

K

P

I

T

D

A

K

G

G

R

S

I

L

L

S

V

V

S

A

G

G

E

F

D

N

V

L

C

V

A

K

L

I

K

K

G

K

T

K

A

D

L

V

R

P

E

L

A

L

R

R

R

R

R

S

I

V

G

G

M

V

V

V

F

F

Q

Q

E

D

Y

Y

N

K

L

L

V

L

E

P

R

K

L

K

S

T

V

V

M

Y

E

E

N

N

V

I

L

A

C

Y

G

A

R

-

L

M

G

E

Y

V

I

I

S

G

P

E

W

N

R

R

A

R

W

N

L

I

R

K

K

R

F

-

P

-

Q

-

E

-

D

-

I

-

D

-

R

R

A

V

F

M

D

E

L

V

L

L

D

D

M

L

V

V

G

G

L

L

A

K

D

H

F

K

A

V

R

R

A

S

R

F

A

P

D

N

E

E

L

L

S

S

G

G

G

G

Q

E

R

Q

Q

Q

R

R

V

I

G

A

I

I

A

A

R

R

A

A

V

I

M

V

Q

N

E

N

P

P

H

K

I

V

L

L

L

I

A

A

D

D

E

E

P

P

T

T

S

G

S

N

L

L

D

D

P

P

K

D

T

N

S

S

V

W

E

E

I

I

M

M

E

N

L

L

L

L

R

E

A

R

V

I

A

-

E

N

K

L

R

Q

D

G

I

T

P

T

V

I

L

L

V

M

N

A

I

T

H

H

D

N

V

S

T

Q

L

I

G

V

R

N

R

T

F

L

S

R

D

H

R

R

V

V

I

I

G

A

M

I

C

E

K

N

G

G

E

R

V

V

L

V

F

R

D

D

D

E

binding phosphoaminophosphonic acid-adenylate ester: Y13 (= Y27), G16 (= G30), A19 (≠ V33), S39 (= S53), G40 (= G54), G42 (= G56), K43 (= K57), S44 (= S58), T45 (= T59)

P0A9R7 Cell division ATP-binding protein FtsE from Escherichia coli (strain K12) (see paper)
37% identity, 83% coverage: 21:240/265 of query aligns to 5:215/222 of P0A9R7

query
sites
P0A9R7

E

E

H

H

L

V

R

S

K

K

E

A

Y

Y

V

L

R

G

G

G

K

R

A

Q

V

A

L

L

K

Q

D

G

I

V

S

T

F

F

T

H

V

M

S

Q

G

P

Q

G

S

E

T

M

T

A

A

F

I

L

I

T

G

G

P

H

S

S

G

G

T

A

G

G

K
|
K

S

S

T

T

L

L

L

L

R

K

C

L

I

I

N
x
C

R

G

L

I

I

E

E

R

P

P

T

S

A

A

G

G

R

K

I

I

L

W

V

F

S

S

G

G

E

H

D

D

V

I

C

T

A

R

L

L

K

K

G

N

T

R

A

E

L

V

R

P

E

F

A

L

R

R

R

R

R

Q

I

I

G

G

M

M

V

I

F

F

Q

Q

E

D

Y

H

N

H

L

L

V

L

E

M

R

D

L

R

S

T

V

V

M

Y

E

D

N

N

V

V

L

A

C

I

G

P

R

L

L

I

G

-

Y

-

I

-

S

-

P

-

W

-

R

-

A

-

W

-

L

I

R

A

K

G

F

A

P

S

Q

G

E

D

D

D

I

I

D

R

R

R

A

R

F

V

D

S

-

A

L

A

L

L

D

D

M

K

V

V

G

G

L

L

A

L

D

D

F

K

A

A

R

K

A

N

R

F

A

P

D

I

E

Q

L

L

S

S

G

G

G

G

Q

E

R

Q

Q

Q

R

R

V

V

G

G

I

I

A

A

R

R

A

A

V

V

M

V

Q

N

E

K

P

P

H

A

I

V

L

L

L

L

A

A

D

D

E

E

P

P

T

T

S

G

S

N

L

L

D

D

P

D

K

A

T

L

S

S

V

E

E

G

I

I

M

L

E

R

L

L

L

F

R

E

A

E

V

F

A

-

E

N

K

R

R

V

D

G

I

V

P

T

V

V

L

L

V

M

N

A

I

T

H

H

D

D

V

I

T

N

L

L

G

I

R

S

R

R

F

R

S

S

D

Y

R

R

V

M

I

L

G

T

M

L

C

S

K

D

G

G

E

H

V

L

K41 (= K57) mutation to R: Does not bind ATP.
C49 (≠ N65) mutation to A: Prevents dimer formation. Does not alter ATP-binding.

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
37% identity, 80% coverage: 18:229/265 of query aligns to 6:213/233 of P75957

query
sites
P75957

L

L

V

Q

V

C

E

D

H

N

L

L

R

C

K

K

E

R

Y

Y

V

Q

R

E

G

G

K

S

A

V

-

Q

-

T

-

D

V

V

L

L

K

H

D

N

I

V

S

S

F

F

T

S

V

V

S

G

G

E

Q

G

S

E

T

M

T

M

A

A

I

I

I

V

G
|
G

P

S

S

S

G

G

T

S

G

G

K

K

S

S

T

T

L

L

L

L

R

H

C

L

I

L

N

G

R

G

L

L

I

D

E

T

P

P

T

T

A

S

G

G

R

D

I

V

L

I

V

F

S

N

G

G

E

Q

D

P

V

M

C

S

A

K

L

L

K

S

G

S

T

A

A

A

L

K

R

A

E

E

A

L

R

R

-

N

R

Q

R

K

I

L

G

G

M

F

V

I

F

Y

Q

Q

E

F

Y

H

N

H

L

L

V

L

E

P

R

D

L

F

S

T

V

A

M

L

E

E

N

N

V

V

L

A

C

M

G

P

R

L

L

L

G

-

Y

-

I

-

S

-

P

-

W

-

R

-

A

-

W

I

L

G

R

K

K

K

F

K

P

P

Q

A

E

E

D

I

I

N

D

S

R

R

A

A

F

L

D

E

L

M

L

L

D

K

M

A

V

V

G

G

L

L

A

D

D

H

F

R

A

A

R

N

A

H

R

R

A

P

D

S

E

E

L

L

S

S

G

G

G

G

Q

E

R

R

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

V

L

M

V

Q

N

E

N

P

P

H

R

I

L

L

V

L

L

A

A

D

D

E

E

P

P

T

T

S

G

S

N

L

L

D

D

P

A

K

R

T

N

S

A

V

D

E

S

I

I

M

F

E

Q

L

L

L

L

R

G

A

E

V

L

A

N

E

R

K

L

R

Q

D

G

I

T

P

A

V

F

L

L

V

V

N

V

I

T

H

H

D

D

V

L

T

Q

L

L

G

A

R

K

R

R

F

M

S

S

G42 (= G51) mutation to D: Loss of lipoprotein release when overexpressed.

7mdyC Lolcde nucleotide-bound
37% identity, 80% coverage: 18:229/265 of query aligns to 3:210/226 of 7mdyC

query
sites
7mdyC

L

L

V

Q

V

C

E

D

H

N

L

L

R

C

K

K

E

R

Y
|
Y

V

Q

R

E

G

G

K

S

A

V

-

Q

-

T

-

D

V

V

L

L

K

H

D

N

I

V

S

S

F

F

T

S

V

V

S

G

G

E

Q

G

S

E

T

M

T

M

A

A

I

I

I

V

G

G

P

S

S

S

G
|
G

T
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

L

R

H

C

L

I

L

N

G

R

G

L

L

I

D

E

T

P

P

T

T

A

S

G

G

R

D

I

V

L

I

V

F

S

N

G

G

E

Q

D

P

V

M

C

S

A

K

L

L

K

S

G

S

T

A

A

A

L

K

R

A

E

E

A

L

R

R

-

N

R

Q

R

K

I

L

G

G

M

F

V

I

F

Y

Q
|
Q

E

F

Y

H

N

H

L

L

V

L

E

P

R

D

L

F

S

T

V

A

M

L

E

E

N

N

V

V

L

A

C

M

G

P

R

L

L

L

G

-

Y

-

I

-

S

-

P

-

W

-

R

-

A

-

W

I

L

G

R

K

K

K

F

K

P

P

Q

A

E

E

D

I

I

N

D

S

R

R

A

A

F

L

D

E

L

M

L

L

D

K

M

A

V

V

G

G

L

L

A

D

D

H

F

R

A

A

R

N

A
x
H

R

R

A

P

D

S

E
|
E

L

L

S
|
S

G
|
G

G
|
G

Q

E

R

R

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

V

L

M

V

Q

N

E

N

P

P

H

R

I

L

L

V

L

L

A

A

D

D

E
|
E

P

P

T

T

S

G

S
x
N

L

L

D

D

P

A

K

R

T

N

S

A

V

D

E

S

I

I

M

F

E

Q

L

L

L

L

R

G

A

E

V

L

A

N

E

R

K

L

R

Q

D

G

I

T

P

A

V

F

L

L

V

V

N

V

I

T

H
|
H

D

D

V

L

T

Q

L

L

G

A

R

K

R

R

F

M

S

S

binding adp orthovanadate: Y12 (= Y27), G42 (= G54), S43 (≠ T55), G44 (= G56), K45 (= K57), S46 (= S58), T47 (= T59), Q91 (= Q102), H138 (≠ A157), E142 (= E161), S144 (= S163), G145 (= G164), G146 (= G165), E168 (= E187), N172 (≠ S191), H201 (= H220)
binding magnesium ion: S46 (= S58), Q91 (= Q102)

7arlD Lolcde in complex with lipoprotein and adp (see paper)
37% identity, 80% coverage: 18:229/265 of query aligns to 3:210/222 of 7arlD

query
sites
7arlD

L

L

V

Q

V

C

E

D

H

N

L

L

R

C

K

K

E

R

Y

Y

V

Q

R

E

G

G

K

S

A

V

-

Q

-

T

-

D

V

V

L

L

K

H

D

N

I

V

S

S

F

F

T

S

V

V

S

G

G

E

Q

G

S

E

T

M

T

M

A

A

I

I

I

V

G

G

P

S

S

S

G
|
G

T

S

G
|
G

K
|
K

S
|
S

T

T

L

L

L

L

R

H

C

L

I

L

N

G

R

G

L

L

I

D

E

T

P

P

T

T

A

S

G

G

R

D

I

V

L

I

V

F

S

N

G

G

E

Q

D

P

V

M

C

S

A

K

L

L

K

S

G

S

T

A

A

A

L

K

R

A

E

E

A

L

R

R

-

N

R

Q

R

K

I

L

G

G

M

F

V

I

F

Y

Q

Q

E

F

Y

H

N

H

L

L

V

L

E

P

R

D

L

F

S

T

V

A

M

L

E

E

N

N

V

V

L

A

C

M

G

P

R

L

L

L

G

-

Y

-

I

-

S

-

P

-

W

-

R

-

A

-

W

I

L

G

R

K

K

K

F

K

P

P

Q

A

E

E

D

I

I

N

D

S

R

R

A

A

F

L

D

E

L

M

L

L

D

K

M

A

V

V

G

G

L

L

A

D

D

H

F

R

A

A

R

N

A

H

R

R

A

P

D

S

E

E

L

L

S

S

G

G

G

G

Q

E

R

R

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

V

L

M

V

Q

N

E

N

P

P

H

R

I

L

L

V

L

L

A

A

D

D

E

E

P

P

T

T

S

G

S

N

L

L

D

D

P

A

K

R

T

N

S

A

V

D

E

S

I

I

M

F

E

Q

L

L

L

L

R

G

A

E

V

L

A

N

E

R

K

L

R

Q

D

G

I

T

P

A

V

F

L

L

V

V

N

V

I

T

H

H

D

D

V

L

T

Q

L

L

G

A

R

K

R

R

F

M

S

S

binding adenosine-5'-diphosphate: G42 (= G54), G44 (= G56), K45 (= K57), S46 (= S58)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
31% identity, 93% coverage: 13:259/265 of query aligns to 7:246/265 of P07821

query
sites
P07821

H

H

G

S

D

D

K

T

S

T

L

F

V

A

V

L

E

R

H

N

L

I

R

S

K

F

E

R

Y

-

V

V

R

P

G

G

K

R

A

T

V

L

L

L

K

H

D

P

I

L

S

S

F

L

T

T

V

F

S

P

G

A

Q

G

S

K

T

V

T

T

A

G

I

L

I

I

G

G

P

H

S

N

G

G

T

S

G

G

K
|
K

S

S

T

T

L

L

L

L

R

K

C

M

I

L

N

G

R

R

L

H

I

Q

E

P

P

P

T

S

A

E

G

G

R

E

I

I

L

L

V

L

S

-

G

-

E

-

D

D

V

A

C

Q

A

P

L

L

K

E

G

S

T

W

A

S

L

S

R

K

E

A

A

F

R

A

R

R

R

K

I

V

G

A

M

Y

V

L

F

P

Q

Q

E

Q

Y

L

N

P

L

P

V

A

E

E

R

G

L

M

S

T

V

V

M

R

E

E

N

L

V

V

L

A

C

I

G

G

R

R

L

Y

G

-

Y

-

I

-

S

-

P

P

W

W

R

H

A

G

W

A

L

L

R

G

K

R

F

F

P

G

Q

A

E

A

D

D

I

R

D

E

R

K

A

V

F

E

D

E

L

A

L

I

D

S

M

L

V

V

G

G

L

L

A

K

D

P

F

L

A

A

R

H

A

R

R

L

A

V

D

D

E

S

L

L

S

S

G

G

G

G

Q

E

R

R

Q

Q

R

R

V

A

G

W

I

I

A

A

R

M

A

L

V

V

M

A

Q

Q

E

D

P

S

H

R

I

C

L

L

L

L

A

L

D
|
D

E
|
E

P

P

T

T

S

S

S

A

L

L

D

D

P

I

K

A

T

H

S

Q

V

V

E

D

I

V

M

L

E

S

L

L

L

V

R

H

A

R

V

L

A

S

E

Q

K

E

R

R

D

G

I

L

P

T

V

V

L

I

V

A

N

V

I

L

H

H

D

D

V

I

T

N

L

M

G

A

R

A

R

R

F

Y

S

C

D

D

R

Y

V

L

I

V

G

A

M

L

C

R

K

G

G

G

E

E

V

M

L

I

F

A

D

Q

D

G

V

T

P

P

S

A

A

E

-

I

L

M

Q

R

D

G

D

E

H

T

L

L

K

E

Q

M

I

I

Y

Y

G

G

K50 (= K57) mutation to Q: Lack of activity.
D172 (= D186) mutation to E: Lack of activity.
E173 (= E187) mutation to A: Lack of activity.

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
37% identity, 80% coverage: 18:229/265 of query aligns to 5:212/229 of 7v8iD

query
sites
7v8iD

L

L

V

Q

V

C

E

D

H

N

L

L

R

C

K

K

E

R

Y

Y

V

Q

R

E

G

G

K

S

A

V

-

Q

-

T

-

D

V
|
V

L

L

K

H

D

N

I

V

S

S

F

F

T

S

V

V

S

G

G

E

Q

G

S

E

T

M

T

M

A

A

I

I

I

V

G

G

P

S

S
|
S

G
|
G

T

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

L

R

H

C

L

I

L

N

G

R

G

L

L

I

D

E

T

P

P

T

T

A

S

G

G

R

D

I

V

L

I

V

F

S

N

G

G

E

Q

D

P

V

M

C

S

A

K

L

L

K

S

G

S

T

A

A

A

L

K

R

A

E

E

A

L

R

R

-

N

R

Q

R

K

I

L

G

G

M

F

V

I

F

Y

Q
|
Q

E

F

Y

H

N

H

L

L

V

L

E

P

R

D

L

F

S

T

V

A

M

L

E

E

N

N

V

V

L

A

C

M

G

P

R

L

L

L

G

-

Y

-

I

-

S

-

P

-

W

-

R

-

A

-

W

I

L

G

R

K

K

K

F

K

P

P

Q

A

E

E

D

I

I

N

D

S

R

R

A

A

F

L

D

E

L

M

L

L

D

K

M

A

V

V

G

G

L

L

A

D

D

H

F
x
R

A

A

R

N

A
x
H

R

R

A

P

D

S

E
|
E

L

L

S
|
S

G

G

G
|
G

Q
x
E

R

R

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

V

L

M

V

Q

N

E

N

P

P

H

R

I

L

L

V

L

L

A

A

D

D

E

Q

P

P

T

T

S

G

S

N

L

L

D

D

P

A

K

R

T

N

S

A

V

D

E

S

I

I

M

F

E

Q

L

L

L

L

R

G

A

E

V

L

A

N

E

R

K

L

R

Q

D

G

I

T

P

A

V

F

L

L

V

V

N

V

I

T

H
|
H

D

D

V

L

T

Q

L

L

G

A

R

K

R

R

F

M

S

S

binding phosphoaminophosphonic acid-adenylate ester: V23 (= V33), S43 (= S53), G44 (= G54), G46 (= G56), K47 (= K57), S48 (= S58), T49 (= T59), Q93 (= Q102), R137 (≠ F154),