SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 207964 MicrobesOnline__882:207964 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

P0AAC6 Modulator of FtsH protease YccA from Escherichia coli (strain K12) (see 2 papers)
30% identity, 50% coverage: 117:233/233 of query aligns to 113:219/219 of P0AAC6

query
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P0AAC6

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Sites not aligning to the query:

1:12 MDRIVSSSHDRT→MTMITPSLHDRI: Protein is as sensitive to FtsH degradation as the wild-type.
5:12 mutation Missing: In yccA11; increases the half-life of overexpressed SecY, F(0) ATP synthase subunit a and itself in an HflKC-dependent fashion, but has no effect on degradation of cytosolic substrates sigma-32 or the phage lambda cII protein. Partially dominant over the wild-type allele. This protein interacts with but is not degraded by FtsH.

Q91VC9 Growth hormone-inducible transmembrane protein; Mitochondrial morphology and cristae structure 1; MICS1 from Mus musculus (Mouse) (see paper)
26% identity, 96% coverage: 11:233/233 of query aligns to 124:345/346 of Q91VC9

query
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Q91VC9

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D326 (= D216) mutation to R: Impaired protein stability. Skeletal muscle mitochondria are swollen, exhibiting a disrupted cristae structure.

Query Sequence

>207964 MicrobesOnline__882:207964
MYNRTMGSAARVEATNAYMRGVYSWMTLGLAVTAAAAWGVASSEALIAFIFGNTFVFFGL
IIAEFALVIGISAGIARLSAGMASGLFLLYSALNGLTLSSILLVYAQSAVFQAFITTAGM
FAVMSIYGATTKRDLTSMGSFLMMGLFGIILASVVNIFMRSSMMEFIISAVGVLVFTGLT
AYDTQKLKAFGENAPMDDAVAIRRGTILGALTLYLDFVNLFIMMVRLFGSSRD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory