SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 208563 DVU3048 aspartate-semialdehyde dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q04797 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Bacillus subtilis (strain 168) (see paper)
56% identity, 96% coverage: 6:339/349 of query aligns to 5:346/346 of Q04797

query
sites
Q04797

L

L

V

H

V

V

A

A

V

V

G

G

A

A

T

T

G

G

A

A

V

V

G

G

R

Q

E

Q

M

M

L

L

K

K

T

T

L

L

E

E

G

D

R

R

D

N

F

F

P

E

A

M

A

D

E

T

V

L

I

T

P

L

F

L

A

S

S

S

A

K

R

R

S

S

A

A

G

G

S

T

K

K

V

V

P

T

F

F

R

K

D

G

G

Q

E

E

L

L

T

T

V

V

R

Q

E

E

L

A

T

S

E

P

D

E

C

S

F

F

T

E

G

G

I

V

D

N

I

I

A

A

L

L

F

F

S

S

A

A

G

G

S

S

T

V

S

S

E

Q

K

A

F

L

A

A

P

P

H

E

A

A

A

V

K

K

A

R

G

G

C

A

V

I

V

V

I

I

D

D

N

N

S

T

S
|
S

A

A

W

F

R

R

M

M

D

D

A

E

R

N

C

T

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

E

H

A

H

D

L

L

E

H

A

E

H

H

N

N

G

G

I

I

A

A

N

N

P

P

N

N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

V

V

A

A

A

L

L

K

E

P

P

L

I

H

R

D

K

A

A

A
x
Y

R

G

I

L

K

N

R

K

V

V

I

I

V

V

S

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G

G

T

A

G

G

Q

N

K

E

A

A

I

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D

K

E

E

L

L

D

Y

R

S

Q

Q

V

T

R

Q

Q

A

M

I

F

L

N

N

M

K

M

E

E

E

P

I

E

E

V

P

Q

E

V

I

Y

M

P

P

-

V

-

K

-

G

-

D

-

K

-

H

H

Y

R

Q

I

I

A

A

F

F

N

N

C

A

L

I

P

P

H

Q

I

I

D

D

V

K

F

F

L

Q

D

D

N

N

D

G

Y

Y

T

T

K

F

E

E

M

M

K

K

M

M

V

I

H

N

E

E

T

T

V

K

K

K

I

I

M

M

D

H

D

M

P

P

S

D

V

L

K

Q

V

V

T

A

A

A

T

T

C

C

V

V

R

R

V

L

P

P

V

I

F

Q

Y

T

G

G

H

H

S

S

E

E

S

S

V

V

N

Y

I

I

E

E

T

I

E

D

K

R

-

D

K

D

L

A

T

T

A

V

R

E

D

D

A

I

R

K

A

N

I

L

L

L

S

K

Q

E

A

A

P

P

G

G

V

V

Q

T

V

L

Y

Q

D

D

N

D

P

P

G

S

Q

Q

K

Q

M

L

Y

Y

P

P

M

M

P

P

I

A

D

D

A

A

A

V

G

G

E

K

D

N

A

D

T

V

F

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

E

L

T

D

I

R

E

A

N

N

G

G

L

F

N

H

L

L

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G

G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

E

S

L

L

I

K

D

K

R

L

D

N

L

L

V

V

S98 (= S99) modified: Phosphoserine
Y146 (≠ A147) modified: Phosphotyrosine

4r5hA Crystal structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide-adenine-dinucleotide-phosphate and 3-carboxy-propenyl- phthalic acid (see paper)
54% identity, 95% coverage: 8:339/349 of query aligns to 4:346/359 of 4r5hA

query
sites
4r5hA

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

R

A

E

Q

M

M

L

I

K

K

T

M

L

L

E

E

G

E

R

S

D

T

F

L

P

P

A

I

A

D

E

K

V

I

I

R

P

Y

F

L

A
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A

S
|
S

A

A

R
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R

S
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S

A

A

G

G

S

K

K

S

V

L

P

K

F

F

R

K

D

D

G

Q

E

D

L

I

T

T

V

I

R

E

E

E

L
x
T

T

T

E

E

D

T

C

A

F

F

T

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S
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S

A
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A

G
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G

G
x
S

S

S

T
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T

S

S

E

A

K

K

F

Y

A

A

P

P

H

Y

A

A

A

V

K

K

A

A

G

G

C

V

V

V

I

V

D

D

N
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N

S
x
T

S
|
S

A

Y

W

F

R
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R

M

Q

D

N

A

P

R

D

C

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

H

A

L

L

E

D

A

A

H

H

N

N

G

G

I

I

A

A

N

C

P
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P

N
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N

C
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C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

A

K

A

W

R

G

I

L

K

D

R

R

V

I

I

I

V

V

S

S

T

T

Y

Y

Q
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Q

A

A

V

V

S

S

G
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G

T

A

G
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G

Q
x
M

K

G

A

A

I

I

D

L

E

E

L

T

D

Q

R

R

Q

E

V

L

R

R

Q

E

M

V

F

L

N

N

-

D

M

G

M

V

E

K

P

P

-

C

-

D

-

L

E

H

V

A

Q

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

H

Y

R

P

I

I

A

A

F

F

N

N

C

A

L

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

D

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

H

K

E

E

T

T

V

K

K

K

I

I

M

M

D

E

D

D

P

D

S

S

V

I

K

A

V

V

T

S

A

A

T

T

C

C

V

V

R
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R

V

I

P

P

V

V

F

L

Y

S

G

A

H
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H

S

S

E

E

S

S

V

V

N

Y

I

I

E

E

T

T

E

K

K

E

K

V

L

A

T

P

A

I

R

E

D

E

A

V

R

K

A

A

I

A

L

I

S

A

Q

A

A

F

P

P

G

G

V

A

Q

V

V

L

Y

E

D

D

N

D

P

V

G

A

Q

H

K

Q

M

I

Y

Y

P

P

M

Q

P

A

I

I

D

N

A

A

A

V

G

G

E

S

D

R

A

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

E

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G
|
G

A
|
A

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

E

T

L

L

I

H

D

E

R

R

D

G

L

L

V

V

binding 3-[(1E)-3-carboxyprop-1-en-1-yl]benzene-1,2-dicarboxylic acid: S73 (≠ G77), T94 (≠ S98), S95 (= S99), R98 (= R102), N126 (= N130), C127 (= C131), Q154 (= Q158), G158 (= G162), E219 (= E212), K222 (= K215), R244 (= R237), H251 (= H244)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), V13 (= V17), A35 (= A39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), G72 (= G76), T75 (= T79), N93 (= N97), T94 (≠ S98), R98 (= R102), P125 (= P129), N126 (= N130), C127 (= C131), G160 (= G164), M161 (≠ Q165), G328 (= G321), A329 (= A322)

4r4jA Crystal structure of complex sp_asadh with 3-carboxypropyl-phthalic acid and nicotinamide adenine dinucleotide phosphate (see paper)
54% identity, 95% coverage: 8:339/349 of query aligns to 4:346/359 of 4r4jA

query
sites
4r4jA

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

R

A

E

Q

M

M

L

I

K

K

T

M

L

L

E

E

G

E

R

S

D

T

F

L

P

P

A

I

A

D

E

K

V

I

I

R

P

Y

F

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

S

K

K

S

V

L

P

K

F

F

R

K

D

D

G

Q

E

D

L

I

T

T

V

I

R

E

E

E

L
x
T

T

T

E

E

D

T

C

A

F

F

T

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G
x
S

S

S

T
|
T

S

S

E

A

K

K

F

Y

A

A

P

P

H

Y

A

A

A

V

K

K

A

A

G

G

C

V

V

V

I

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

W

F

R
|
R

M

Q

D

N

A

P

R

D

C

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

H

A

L

L

E

D

A

A

H

H

N

N

G

G

I

I

A

A

N

C

P
|
P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

A

K

A

W

R

G

I

L

K

D

R

R

V

I

I

I

V

V

S

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
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G

T

A

G
|
G

Q
x
M

K

G

A

A

I

I

D

L

E

E

L

T

D

Q

R

R

Q

E

V

L

R

R

Q

E

M

V

F

L

N

N

-

D

M

G

M

V

E

K

P

P

-

C

-

D

-

L

E

H

V

A

Q

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

H

Y

R

P

I

I

A

A

F

F

N

N

C

A

L

L

P

P

H

Q

I
|
I

D

D

V

V

F

F

L

T

D

D

N

N

D

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K
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K

M

M

V

T

H

K

E

E

T

T

V

K

K

K

I

I

M

M

D

E

D

D

P

D

S

S

V

I

K

A

V

V

T

S

A

A

T

T

C

C

V

V

R
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R

V

I

P

P

V

V

F

L

Y

S

G

A

H
|
H

S

S

E

E

S

S

V

V

N

Y

I

I

E

E

T

T

E

K

K

E

K

V

L

A

T

P

A

I

R

E

D

E

A

V

R

K

A

A

I

A

L

I

S

A

Q

A

A

F

P

P

G

G

V

A

Q

V

V

L

Y

E

D

D

N

D

P

V

G

A

Q

H

K

Q

M

I

Y

Y

P

P

M

Q

P

A

I

I

D

N

A

A

A

V

G

G

E

S

D

R

A

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

E

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G
|
G

A
|
A

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

E

T

L

L

I

H

D

E

R

R

D

G

L

L

V

V

binding 3-(3-carboxypropyl)benzene-1,2-dicarboxylic acid: S73 (≠ G77), T94 (≠ S98), S95 (= S99), R98 (= R102), N126 (= N130), C127 (= C131), Q154 (= Q158), G158 (= G162), I208 (= I201), E219 (= E212), K222 (= K215), R244 (= R237), H251 (= H244)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), V13 (= V17), A35 (= A39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), G72 (= G76), T75 (= T79), N93 (= N97), T94 (≠ S98), R98 (= R102), P125 (= P129), N126 (= N130), C127 (= C131), G160 (= G164), M161 (≠ Q165), G328 (= G321), A329 (= A322)

3pyxB Crystals structure of aspartate beta-semialdehyde dehydrogenase complex with NADP and 2-aminoterephthalate (see paper)
54% identity, 95% coverage: 8:339/349 of query aligns to 4:346/359 of 3pyxB

query
sites
3pyxB

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

R

A

E

Q

M

M

L

I

K

K

T

M

L

L

E

E

G

E

R

S

D

T

F

L

P

P

A

I

A

D

E

K

V

I

I

R

P

Y

F

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

S

K

K

S

V

L

P

K

F

F

R

K

D

D

G

Q

E

D

L

I

T

T

V

I

R

E

E

E

L
x
T

T

T

E

E

D

T

C

A

F

F

T

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G

S

S

S

T
|
T

S

S

E

A

K

K

F

Y

A

A

P

P

H

Y

A

A

A

V

K

K

A

A

G

G

C

V

V

V

I

V

D

D

N
|
N

S
x
T

S

S

A

Y

W

F

R
|
R

M

Q

D

N

A

P

R

D

C

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

H

A

L

L

E

D

A

A

H

H

N

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

A

K

A

W

R

G

I

L

K

D

R

R

V

I

I

I

V

V

S

S

T

T

Y

Y

Q

Q

A

A

V

V

S
|
S

G
|
G

T
x
A

G
|
G

Q
x
M

K

G

A

A

I

I

D

L

E

E

L

T

D

Q

R

R

Q

E

V

L

R

R

Q

E

M

V

F

L

N

N

-

D

M

G

M

V

E

K

P

P

-

C

-

D

-

L

E

H

V

A

Q

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

H

Y

R

P

I

I

A

A

F

F

N

N

C

A

L

L

P

P

H

Q

I
|
I

D
|
D

V

V

F

F

L

T

D

D

N

N

D

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

H

K

E

E

T

T

V

K

K

K

I

I

M

M

D

E

D

D

P

D

S

S

V

I

K

A

V

V

T

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

F

L

Y

S

G

A

H

H

S

S

E

E

S

S

V

V

N

Y

I

I

E

E

T

T

E

K

K

E

K

V

L

A

T

P

A

I

R

E

D

E

A

V

R

K

A

A

I

A

L

I

S

A

Q

A

A

F

P

P

G

G

V

A

Q

V

V

L

Y

E

D

D

N

D

P

V

G

A

Q

H

K

Q

M

I

Y

Y

P

P

M

Q

P

A

I

I

D

N

A

A

A

V

G

G

E

S

D

R

A

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

E

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N
|
N

I
x
L

R

L

K

K

G
|
G

A
|
A

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

E

T

L

L

I

H

D

E

R

R

D

G

L

L

V

V

binding 2-aminobenzene-1,4-dicarboxylic acid: R98 (= R102), N126 (= N130), C127 (= C131), G158 (= G162), A159 (≠ T163), I208 (= I201), D209 (= D202), E219 (= E212), K222 (= K215), R244 (= R237)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), V13 (= V17), A35 (= A39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), G72 (= G76), T75 (= T79), N93 (= N97), T94 (≠ S98), C127 (= C131), S157 (= S161), G158 (= G162), G160 (= G164), M161 (≠ Q165), N324 (= N317), L325 (≠ I318), G328 (= G321), A329 (= A322)

3q11A Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with NADP and aspartyl beta- difluorophosphonate (see paper)
54% identity, 95% coverage: 8:339/349 of query aligns to 4:346/358 of 3q11A

query
sites
3q11A

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V

V

G

G

R

A

E

Q

M

M

L

I

K

K

T

M

L

L

E

E

G

E

R

S

D

T

F

L

P

P

A

I

A

D

E

K

V

I

I

R

P

Y

F

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

S

K

K

S

V

L

P

K

F

F

R

K

D

D

G

Q

E

D

L

I

T

T

V

I

R

E

E

E

L
x
T

T

T

E

E

D

T

C

A

F

F

T

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G

G

G

S

S

S

T
|
T

S

S

E

A

K

K

F

Y

A

A

P

P

H

Y

A

A

A

V

K

K

A

A

G

G

C

V

V

V

I

V

D

D

N

N

S

T

S

S

A

Y

W

F

R
|
R

M

Q

D

N

A

P

R

D

C

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

H

A

L

L

E

D

A

A

H

H

N

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

A

K

A

W

R

G

I

L

K

D

R

R

V

I

I

I

V

V

S

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

T

A

G
|
G

Q
x
M

K
x
G

A

A

I

I

D

L

E

E

L

T

D

Q

R

R

Q

E

V

L

R

R

Q

E

M

V

F

L

N

N

-

D

M

G

M

V

E

K

P

P

-

C

-

D

-

L

E

H

V

A

Q

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

H

Y

R

P

I

I

A

A

F

F

N

N

C

A

L

L

P

P

H

Q

I
|
I

D

D

V

V

F

F

L

T

D

D

N

N

D

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

H

K

E

E

T

T

V

K

K

K

I

I

M

M

D

E

D

D

P

D

S

S

V

I

K

A

V

V

T

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

F

L

Y

S

G

A

H
|
H

S

S

E

E

S

S

V

V

N

Y

I

I

E

E

T

T

E

K

K

E

K

V

L

A

T

P

A

I

R

E

D

E

A

V

R

K

A

A

I

A

L

I

S

A

Q

A

A

F

P

P

G

G

V

A

Q

V

V

L

Y

E

D

D

N

D

P

V

G

A

Q

H

K

Q

M

I

Y

Y

P

P

M

Q

P

A

I

I

D

N

A

A

A

V

G

G

E

S

D

R

A

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

E

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G

G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

E

T

L

L

I

H

D

E

R

R

D

G

L

L

V

V

binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), A35 (= A39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), T75 (= T79), G160 (= G164), M161 (≠ Q165), G162 (≠ K166)
binding 5,5-difluoro-4-oxo-5-phosphono-D-norvaline: R98 (= R102), N126 (= N130), C127 (= C131), Q154 (= Q158), G158 (= G162), I208 (= I201), E219 (= E212), K222 (= K215), R244 (= R237), H251 (= H244)

4r54A Complex crystal structure of sp-aspartate-semialdehyde-dehydrogenase with 3-carboxy-ethyl-phthalic acid (see paper)
54% identity, 95% coverage: 8:339/349 of query aligns to 4:346/357 of 4r54A

query
sites
4r54A

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

R

A

E

Q

M

M

L

I

K

K

T

M

L

L

E

E

G

E

R

S

D

T

F

L

P

P

A

I

A

D

E

K

V

I

I

R

P

Y

F

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

S

K

K

S

V

L

P

K

F

F

R

K

D

D

G

Q

E

D

L

I

T

T

V

I

R

E

E

E

L
x
T

T

T

E

E

D

T

C

A

F

F

T

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G
x
S

S

S

T
|
T

S

S

E

A

K

K

F

Y

A

A

P

P

H

Y

A

A

A

V

K

K

A

A

G

G

C

V

V

V

I

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

W

F

R
|
R

M

Q

D

N

A

P

R

D

C

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

H

A

L

L

E

D

A

A

H

H

N

N

G

G

I

I

A

A

N

C

P
|
P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

A

K

A

W

R

G

I

L

K

D

R

R

V

I

I

I

V

V

S

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G

G

T

A

G
|
G

Q
x
M

K

G

A

A

I

I

D

L

E

E

L

T

D

Q

R

R

Q

E

V

L

R

R

Q

E

M

V

F

L

N

N

-

D

M

G

M

V

E

K

P

P

-

C

-

D

-

L

E

H

V

A

Q

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

H

Y

R

P

I

I

A

A

F

F

N

N

C

A

L

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

D

D

Y

Y

T

T

K

Y

E

E

M

M

K
|
K

M

M

V

T

H

K

E

E

T

T

V

K

K

K

I

I

M

M

D

E

D

D

P

D

S

S

V

I

K

A

V

V

T

S

A

A

T

T

C

C

V

V

R

R

V

I

P

P

V

V

F

L

Y

S

G

A

H

H

S

S

E

E

S

S

V

V

N

Y

I

I

E

E

T

T

E

K

K

E

K

V

L

A

T

P

A

I

R

E

D

E

A

V

R

K

A

A

I

A

L

I

S

A

Q

A

A

F

P

P

G

G

V

A

Q

V

V

L

Y

E

D

D

N

D

P

V

G

A

Q

H

K

Q

M

I

Y

Y

P

P

M

Q

P

A

I

I

D

N

A

A

A

V

G

G

E

S

D

R

A

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

E

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G
|
G

A
|
A

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

E

T

L

L

I

H

D

E

R

R

D

G

L

L

V

V

binding 3-(2-carboxyethyl)benzene-1,2-dicarboxylic acid: G72 (= G76), S73 (≠ G77), T94 (≠ S98), S95 (= S99), R98 (= R102), N126 (= N130), K222 (= K215)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), V13 (= V17), A35 (= A39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), G72 (= G76), T75 (= T79), N93 (= N97), T94 (≠ S98), R98 (= R102), P125 (= P129), N126 (= N130), C127 (= C131), G160 (= G164), M161 (≠ Q165), G328 (= G321), A329 (= A322)

4r41A Complex crystal structure of 4-nitro-2-phosphono-benzoic acid with sp- aspartate-semialdehyde dehydrogenase and nicotinamide-dinucleotide (see paper)
54% identity, 95% coverage: 8:339/349 of query aligns to 4:346/357 of 4r41A

query
sites
4r41A

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

R

A

E

Q

M

M

L

I

K

K

T

M

L

L

E

E

G

E

R

S

D

T

F

L

P

P

A

I

A

D

E

K

V

I

I

R

P

Y

F

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

S

K

K

S

V

L

P

K

F

F

R

K

D

D

G

Q

E

D

L

I

T

T

V

I

R

E

E

E

L
x
T

T

T

E

E

D

T

C

A

F

F

T

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G
x
S

S

S

T
|
T

S

S

E

A

K

K

F

Y

A

A

P

P

H

Y

A

A

A

V

K

K

A

A

G

G

C

V

V

V

I

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

W

F

R
|
R

M

Q

D

N

A

P

R

D

C

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

H

A

L

L

E

D

A

A

H

H

N

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

A

K

A

W

R

G

I

L

K

D

R

R

V

I

I

I

V

V

S

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G

G

T

A

G
|
G

Q
x
M

K
x
G

A

A

I

I

D
x
L

E

E

L

T

D

Q

R

R

Q

E

V

L

R

R

Q

E

M

V

F

L

N

N

-

D

M

G

M

V

E

K

P

P

-

C

-

D

-

L

E

H

V

A

Q

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

H

Y

R

P

I

I

A

A

F

F

N

N

C

A

L

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

D

D

Y

Y

T

T

K

Y

E

E

M

M

K
|
K

M

M

V

T

H

K

E

E

T

T

V

K

K

K

I

I

M

M

D

E

D

D

P

D

S

S

V

I

K

A

V

V

T

S

A

A

T

T

C

C

V

V

R

R

V

I

P

P

V

V

F

L

Y

S

G

A

H

H

S

S

E

E

S

S

V

V

N

Y

I

I

E

E

T

T

E

K

K

E

K

V

L

A

T

P

A

I

R

E

D

E

A

V

R

K

A

A

I

A

L

I

S

A

Q

A

A

F

P

P

G

G

V

A

Q

V

V

L

Y

E

D

D

N

D

P

V

G

A

Q

H

K

Q

M

I

Y

Y

P

P

M

Q

P

A

I

I

D

N

A

A

A

V

G

G

E

S

D

R

A

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

E

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G

G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

E

T

L

L

I

H

D

E

R

R

D

G

L

L

V

V

binding 4-nitro-2-phosphonobenzoic acid: V13 (= V17), S70 (= S74), A71 (= A75), G72 (= G76), S73 (≠ G77), N93 (= N97), T94 (≠ S98), S95 (= S99), R98 (= R102), N126 (= N130), K222 (= K215)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), A35 (= A39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), A71 (= A75), T75 (= T79), G160 (= G164), M161 (≠ Q165), G162 (≠ K166), L165 (≠ D169)

4r3nA Crystal structure of the ternary complex of sp-asadh with NADP and 1, 2,3-benzenetricarboxylic acid (see paper)
54% identity, 95% coverage: 8:339/349 of query aligns to 4:346/357 of 4r3nA

query
sites
4r3nA

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

R

A

E

Q

M

M

L

I

K

K

T

M

L

L

E

E

G

E

R

S

D

T

F

L

P

P

A

I

A

D

E

K

V

I

I

R

P

Y

F

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

S

K

K

S

V

L

P

K

F

F

R

K

D

D

G

Q

E

D

L

I

T

T

V

I

R

E

E

E

L
x
T

T

T

E

E

D

T

C

A

F

F

T

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G
x
S

S

S

T
|
T

S

S

E

A

K

K

F

Y

A

A

P

P

H

Y

A

A

A

V

K

K

A

A

G

G

C

V

V

V

I

V

D

D

N
|
N

S
x
T

S
|
S

A

Y

W

F

R
|
R

M

Q

D

N

A

P

R

D

C

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

H

A

L

L

E

D

A

A

H

H

N

N

G

G

I

I

A

A

N

C

P
|
P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

A

K

A

W

R

G

I

L

K

D

R

R

V

I

I

I

V

V

S

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G

G

T

A

G
|
G

Q
x
M

K

G

A

A

I

I

D

L

E

E

L

T

D

Q

R

R

Q

E

V

L

R

R

Q

E

M

V

F

L

N

N

-

D

M

G

M

V

E

K

P

P

-

C

-

D

-

L

E

H

V

A

Q

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

H

Y

R

P

I

I

A

A

F

F

N

N

C

A

L

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

D

D

Y

Y

T

T

K

Y

E

E

M

M

K
|
K

M

M

V

T

H

K

E

E

T

T

V

K

K

K

I

I

M

M

D

E

D

D

P

D

S

S

V

I

K

A

V

V

T

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

F

L

Y

S

G

A

H
|
H

S

S

E

E

S

S

V

V

N

Y

I

I

E

E

T

T

E

K

K

E

K

V

L

A

T

P

A

I

R

E

D

E

A

V

R

K

A

A

I

A

L

I

S

A

Q

A

A

F

P

P

G

G

V

A

Q

V

V

L

Y

E

D

D

N

D

P

V

G

A

Q

H

K

Q

M

I

Y

Y

P

P

M

Q

P

A

I

I

D

N

A

A

A

V

G

G

E

S

D

R

A

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

E

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G
|
G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

E

T

L

L

I

H

D

E

R

R

D

G

L

L

V

V

active site: C127 (= C131), Q154 (= Q158), R244 (= R237), H251 (= H244)
binding benzene-1,2,3-tricarboxylic acid: S73 (≠ G77), T94 (≠ S98), S95 (= S99), R98 (= R102), N126 (= N130), K222 (= K215)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), V13 (= V17), A35 (= A39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), G72 (= G76), T75 (= T79), N93 (= N97), T94 (≠ S98), R98 (= R102), P125 (= P129), N126 (= N130), C127 (= C131), G160 (= G164), M161 (≠ Q165), G328 (= G321)

3q1lA Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with cysteamine bound covalently to cys 128 (see paper)
54% identity, 95% coverage: 8:339/349 of query aligns to 4:346/357 of 3q1lA

query
sites
3q1lA

V

V

A

A

V

V

G

G

A

A

T

T

G

G

A

A

V

V

G

G

R

A

E

Q

M

M

L

I

K

K

T

M

L

L

E

E

G

E

R

S

D

T

F

L

P

P

A

I

A

D

E

K

V

I

I

R

P

Y

F

L

A

A

S

S

A

A

R

R

S

S

A

A

G

G

S

K

K

S

V

L

P

K

F

F

R

K

D

D

G

Q

E

D

L

I

T

T

V

I

R

E

E

E

L

T

T

T

E

E

D

T

C

A

F

F

T

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S

S

A

A

G

G

G

S

S

S

T

T

S

S

E

A

K

K

F

Y

A

A

P

P

H

Y

A

A

A

V

K

K

A

A

G

G

C

V

V

V

I

V

D

D

N

N

S

T

S

S

A

Y

W

F

R

R

M

Q

D

N

A

P

R

D

C

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

H

A

L

L

E

D

A

A

H

H

N

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

A

K

A

W

R

G

I

L

K

D

R

R

V

I

I

I

V

V

S

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

T

A

G

G

Q

M

K

G

A

A

I

I

D

L

E

E

L

T

D

Q

R

R

Q

E

V

L

R

R

Q

E

M

V

F

L

N

N

-

D

M

G

M

V

E

K

P

P

-

C

-

D

-

L

E

H

V

A

Q

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

H

Y

R

P

I

I

A

A

F

F

N

N

C

A

L

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

D

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K

K

M

M

V

T

H

K

E

E

T

T

V

K

K

K

I

I

M

M

D

E

D

D

P

D

S

S

V

I

K

A

V

V

T

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

F

L

Y

S

G

A

H
|
H

S

S

E

E

S

S

V

V

N

Y

I

I

E

E

T

T

E

K

K

E

K

V

L

A

T

P

A

I

R

E

D

E

A

V

R

K

A

A

I

A

L

I

S

A

Q

A

A

F

P

P

G

G

V

A

Q

V

V

L

Y

E

D

D

N

D

P

V

G

A

Q

H

K

Q

M

I

Y

Y

P

P

M

Q

P

A

I

I

D

N

A

A

A

V

G

G

E

S

D

R

A

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

E

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G

G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

E

T

L

L

I

H

D

E

R

R

D

G

L

L

V

V

active site: C127 (= C131), Q154 (= Q158), R244 (= R237), H251 (= H244)
binding 2-amino-ethanethiol: N126 (= N130), C127 (= C131), Q154 (= Q158), G158 (= G162), E219 (= E212)

3pwsA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
54% identity, 95% coverage: 8:339/349 of query aligns to 4:346/357 of 3pwsA

query
sites
3pwsA

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V

V

G

G

R

A

E

Q

M

M

L

I

K

K

T

M

L

L

E

E

G

E

R

S

D

T

F

L

P

P

A

I

A

D

E

K

V

I

I

R

P

Y

F

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

S

K

K

S

V

L

P

K

F

F

R

K

D

D

G

Q

E

D

L

I

T

T

V

I

R

E

E

E

L
x
T

T

T

E

E

D

T

C

A

F

F

T

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G

G

G

S

S

S

T
|
T

S

S

E

A

K

K

F

Y

A

A

P

P

H

Y

A

A

A

V

K

K

A

A

G

G

C

V

V

V

I

V

D

D

N

N

S

T

S

S

A

Y

W

F

R
|
R

M

Q

D

N

A

P

R

D

C

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

H

A

L

L

E

D

A

A

H

H

N

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

A

K

A

W

R

G

I

L

K

D

R

R

V

I

I

I

V

V

S

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

T
x
A

G
|
G

Q
x
M

K

G

A

A

I

I

D

L

E

E

L

T

D

Q

R

R

Q

E

V

L

R

R

Q

E

M

V

F

L

N

N

-

D

M

G

M

V

E

K

P

P

-

C

-

D

-

L

E

H

V

A

Q

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

H

Y

R

P

I

I

A

A

F

F

N

N

C

A

L

L

P

P

H

Q

I
|
I

D

D

V

V

F

F

L

T

D

D

N

N

D

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

H

K

E

E

T

T

V

K

K

K

I

I

M

M

D

E

D

D

P

D

S

S

V

I

K

A

V

V

T

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

F

L

Y

S

G

A

H
|
H

S

S

E

E

S

S

V

V

N

Y

I

I

E

E

T

T

E

K

K

E

K

V

L

A

T

P

A

I

R

E

D

E

A

V

R

K

A

A

I

A

L

I

S

A

Q

A

A

F

P

P

G

G

V

A

Q

V

V

L

Y

E

D

D

N

D

P

V

G

A

Q

H

K

Q

M

I

Y

Y

P

P

M

Q

P

A

I

I

D

N

A

A

A

V

G

G

E

S

D

R

A

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

E

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G

G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

E

T

L

L

I

H

D

E

R

R

D

G

L

L

V

V

binding (2R)-2-aminohexanedioic acid: R98 (= R102), N126 (= N130), C127 (= C131), Q154 (= Q158), G158 (= G162), A159 (≠ T163), I208 (= I201), E219 (= E212), K222 (= K215), R244 (= R237), H251 (= H244)
binding adenosine-2'-5'-diphosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), A35 (= A39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), T75 (= T79), G160 (= G164), M161 (≠ Q165)

3pwkA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
54% identity, 95% coverage: 8:339/349 of query aligns to 4:346/357 of 3pwkA

query
sites
3pwkA

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V

V

G

G

R

A

E

Q

M

M

L

I

K

K

T

M

L

L

E

E

G

E

R

S

D

T

F

L

P

P

A

I

A

D

E

K

V

I

I

R

P

Y

F

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

S

K

K

S

V

L

P

K

F

F

R

K

D

D

G

Q

E

D

L

I

T

T

V

I

R

E

E

E

L
x
T

T

T

E

E

D

T

C

A

F

F

T

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G

G

G

S

S

S

T
|
T

S

S

E

A

K

K

F

Y

A

A

P

P

H

Y

A

A

A

V

K

K

A

A

G

G

C

V

V

V

I

V

D

D

N

N

S

T

S

S

A

Y

W

F

R
|
R

M

Q

D

N

A

P

R

D

C

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

H

A

L

L

E

D

A

A

H

H

N

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

A

K

A

W

R

G

I

L

K

D

R

R

V

I

I

I

V

V

S

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G
|
G

T
x
A

G
|
G

Q
x
M

K

G

A

A

I

I

D

L

E

E

L

T

D

Q

R

R

Q

E

V

L

R

R

Q

E

M

V

F

L

N

N

-

D

M

G

M

V

E

K

P

P

-

C

-

D

-

L

E

H

V

A

Q

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

H

Y

R

P

I

I

A

A

F

F

N

N

C

A

L

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

D

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

H

K

E

E

T

T

V

K

K

K

I

I

M

M

D

E

D

D

P

D

S

S

V

I

K

A

V

V

T

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

F

L

Y

S

G

A

H

H

S

S

E

E

S

S

V

V

N

Y

I

I

E

E

T

T

E

K

K

E

K

V

L

A

T

P

A

I

R

E

D

E

A

V

R

K

A

A

I

A

L

I

S

A

Q

A

A

F

P

P

G

G

V

A

Q

V

V

L

Y

E

D

D

N

D

P

V

G

A

Q

H

K

Q

M

I

Y

Y

P

P

M

Q

P

A

I

I

D

N

A

A

A

V

G

G

E

S

D

R

A

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

E

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G

G

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

E

T

L

L

I

H

D

E

R

R

D

G

L

L

V

V

binding 5'-o-monophosphoryladenylyl(2'->5')adenylyl(2'->5')adenosine: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), A35 (= A39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), T75 (= T79), G160 (= G164), M161 (≠ Q165)
binding trans-cyclohexane-1,4-dicarboxylic acid: R98 (= R102), N126 (= N130), G158 (= G162), A159 (≠ T163), E219 (= E212), K222 (= K215), R244 (= R237)

2gz3A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP and aspartate- semialdehyde (see paper)
54% identity, 95% coverage: 8:339/349 of query aligns to 4:346/357 of 2gz3A

query
sites
2gz3A

V

V

A

A

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

R

A

E

Q

M

M

L

I

K

K

T

M

L

L

E

E

G

E

R

S

D

T

F

L

P

P

A

I

A

D

E

K

V

I

I

R

P

Y

F

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

A

A

G

G

S

K

K

S

V

L

P

K

F

F

R

K

D

D

G

Q

E

D

L

I

T

T

V

I

R

E

E

E

L
x
T

T

T

E

E

D

T

C

A

F

F

T

E

G

G

I

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G

S

S

S

T
|
T

S

S

E

A

K

K

F

Y

A

A

P

P

H

Y

A

A

A

V

K

K

A

A

G

G

C

V

V

V

I

V

D

D

N
|
N

S
x
T

S

S

A

Y

W

F

R

R

M

Q

D

N

A

P

R

D

C

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

H

A

L

L

E

D

A

A

H

H

N

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

A

A

L

L

K

E

P

P

L

V

H

R

D

Q

A

K

A

W

R

G

I

L

K

D

R

R

V

I

I

I

V

V

S

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S
|
S

G
|
G

T

A

G
|
G

Q
x
M

K

G

A

A

I

I

D

L

E

E

L

T

D

Q

R

R

Q

E

V

L

R

R

Q

E

M

V

F

L

N

N

-

D

M

G

M

V

E

K

P

P

-

C

-

D

-

L

E

H

V

A

Q

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

H

Y

R

P

I

I

A

A

F

F

N

N

C

A

L

L

P

P

H

Q

I

I

D

D

V

V

F

F

L

T

D

D

N

N

D

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K

K

M

M

V

T

H

K

E

E

T

T

V

K

K

K

I

I

M

M

D

E

D

D

P

D

S

S

V

I

K

A

V

V

T

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

F

L

Y

S

G

A

H
|
H

S

S

E

E

S

S

V

V

N

Y

I

I

E

E

T

T

E

K

K

E

K

V

L

A

T

P

A

I

R

E

D

E

A

V

R

K

A

A

I

A

L

I

S

A

Q

A

A

F

P

P

G

G

V

A

Q

V

V

L

Y

E

D

D

N

D

P

V

G

A

Q

H

K

Q

M

I

Y

Y

P

P

M

Q

P

A

I

I

D

N

A

A

A

V

G

G

E

S

D

R

A

D

T

T

F

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

E

L

T

D

I

A

E

E

N

K

G

G

L

I

N

H

L

M

W

W

I

V

V

V

A

S

D

D

N
|
N

I
x
L

R

L

K

K

G
|
G

A
|
A

A

A

L

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

E

T

L

L

I

H

D

E

R

R

D

G

L

L

V

V

active site: C127 (= C131), Q154 (= Q158), R244 (= R237), H251 (= H244)
binding (2r)-2-amino-4-oxobutanoic acid: N126 (= N130), C127 (= C131), Q154 (= Q158), G158 (= G162), E219 (= E212), R244 (= R237), H251 (= H244)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), V13 (= V17), A35 (= A39), S36 (= S40), R38