SitesBLAST

O32148 (S)-ureidoglycine--glyoxylate transaminase; UGXT; (S)-ureidoglycine--glyoxylate aminotransferase; Purine catabolism protein PucG; EC 2.6.1.112 from Bacillus subtilis (strain 168) (see paper)
35% identity, 98% coverage: 8:376/376 of query aligns to 7:387/416 of O32148

query
sites
O32148

L

L

G

C

H

T

P

P

L

L

R

R

N

T

I

I

K

-

A

-

L

-

M

M

G

T

D

P

G

G

P

P

S

V

G

E

V

V

P

D

D

P

S

R

V

V

Y

L

S

R

A

V

L

M

A

S

L

T

P

P

T

V

V

V

G

G

P
x
Q

L

F

D

D

P

P

G

A

F

F

D

T

E

G

I

I

L

M

D

N

R

E

L

T

Q

M

G

E

Q

M

L

L

R

R

N

E

I

L

F

F

N

Q

V

T

S

K

D

N

G

R

V

W

C

A

A

Y

V

P

L

I

D

D

G

G

P

T

A

S

A

R

V

A

G

G

M

I

E

E

T

A

C

V

L

L

V

A

N

S

L

V

L

I

E

E

P

P

G

E

E

D

R

D

L

V

L

L

V

I

L

P

D

I

N

Y

G

G

M

R

G

F

G

G

E

Y

R

L

L

L

R

T

E

E

T

I

A

A

Q

E

R

R

L

Y

G

G

L

A

A

N

V

V

D

H

V

M

L

L

T

E

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C

P

E

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W

G

G

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T

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A

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L

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Y

P

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K

A

I

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A

M

M

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H

H

A

G

E

E

S

T

S

S

T

T

G

G

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R

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L

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K

A

A

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E

L

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T

H

E

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D

A

A

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L

F

F

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I

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D

C

A

E

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I

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A

A

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|
K

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G

G

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M

A

A

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D

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A

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T

K

-

V

-

E

-

R

-

G

-

I

-

A

-

T

-

Q

-

A

-

D

-

R

-

A

-

L

-

S

-

G

-

N

-

R

P

P

I

I

P

T

G

S

R

N

Y

Y

F

F

D

D

I

L

A

S

L

Q

H

L

L

E

D

D

Y

Y

W

W

Q

S

G

-

A

E

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R

R

R

V

L

C
x
N

H

H

L

H

M

T

P

E

S

A

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T

N

T

L

M

L

L

Y

Y

A

A

L

L

H

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M

E

A

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L

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E

R

G

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V

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L

L

N

E

E

E

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G

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L

P

E

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F

A

E

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R

H

H

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R

A

H

H

E

E

A

Q

A

L

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A

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A

A

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M

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F

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E

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I

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L

L

A

A

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I

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W

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I

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G

L

T

M

M

G

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H

Y

S

S

A

C

R

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T

K

T

E

N

N

V

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L

A

F

L

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L

L

E

A

A

G

L

L

G

E

R

A

L

V

L

L

Q37 (≠ P43) mutation to H: 5-fold decrease in transamination activity.
K198 (= K204) modified: N6-(pyridoxal phosphate)lysine
N264 (≠ C253) mutation to S: 9-fold decrease in transamination activity.; mutation to Y: Loss of transamination activity.

2huuA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase in complex with alanine (see paper)
37% identity, 94% coverage: 19:372/376 of query aligns to 21:376/385 of 2huuA

query
sites
2huuA

L

L

M

M

G

G

D
x
P

G

G

P

P

S

S

G

N

V

A

P

P

D

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V

Y

L

S

D

A

A

L

M

A

S

L

R

P

P

T

I

V

L

G

G

P
x
H

L

L

D

H

P

P

G

E

F

T

D

L

E

K

I

I

L

M

D

D

R

D

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Q

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G

E

Q

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L

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R

R

N

Y

I

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F

F

N

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T

S

N

D

N

G

I

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A

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T

A

F

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C

L

L

D

S

G

A

P

S

A

G

A

H

V

G

G

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M

M

E

E

T

A

C

T

L

L

V

C

N

N

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L

E

E

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D

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G

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D

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H

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G

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A

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A

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Y

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D

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P

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L

L

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S

L

E

D

Q

E

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I

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R

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D

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A

L

L

A

L

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I

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H

D

K

Y

P

H

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A

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V

L

V

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M

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V

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H

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A

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E

D

S
|
S

S

S

T

T

G

G

V

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R

L

S

Q

P

G

V

L

G

E

A

G

V

V

G

G

D

A

L

L

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C

S

H

M

Q

H

H

G

N

A

C

L

L

F

L

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I

V

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D

D

C

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E

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T

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S

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L

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M

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F

M

M

A

D

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W

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E

A

I

D

D

A

A

L

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F

Y

A

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S

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S

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H

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K
|
K

C

V

L

L

S

G

C

A

P

P

G

G

L

I

A

T

P

P

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A

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F

F

S

S

M

H

R

R

A

A

L

V

D

E

R

R

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Y

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K

R

R

R

N

T

T

P

K

I

V

P

K

G

V

R

Y

Y

Y

F

W

D

D

I

M

A

S

L

L

H

V

L

G

D

D

Y

Y

W

W

Q

G

-

C

-

F

G

G

A

R

P

P

R

R

V

I

C
x
Y

H

H

L

H

M

T

P

I

S

S

V

S

N

T

L

L

Y

Y

A

G

L

L

H

R

M

E

A

A

L

I

E

A

G

M

V

A

L

C

N

E

E

E

G

G

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L

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P

N

A

V

L

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I

A

A

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R

H

H

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E

A

D

A

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H

A

E

K

Q

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T

R

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L

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A

Q

A

D

L

A

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G

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F

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P

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Y

A

A

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W

D

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L

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A

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E

Q

G

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D

A

W

E

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A

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A

K

E

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Y

G

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E

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I

I

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S

G

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x
L

G

G

R

P

L

T

A

A

G

G

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Q

V

V

W

F

R
|
R

I

I

G

G

L

L

M

M

G

G

H

Q

S

N

A

A

R

T

T

T

T

E

N

R

V

V

V

D

A

R

L

V

L

L

E

Q

A

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L

F

binding 1-butanol: H45 (≠ P43), Y257 (≠ C253)
binding alanine: P25 (≠ D23), S155 (= S153), K206 (= K204), L347 (≠ V343), R356 (= R352)

2huiA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase in complex with glyoxylic acid (see paper)
37% identity, 94% coverage: 19:372/376 of query aligns to 21:376/385 of 2huiA

query
sites
2huiA

L

L

M

M

G

G

D
x
P

G

G

P

P

S

S

G

N

V

A

P

P

D

Q

S

R

V

V

Y

L

S

D

A

A

L

M

A

S

L

R

P

P

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I

V

L

G

G

P

H

L

L

D

H

P

P

G

E

F

T

D

L

E

K

I

I

L

M

D

D

R

D

L

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Q

K

G

E

Q

G

L

V

R

R

N

Y

I

L

F

F

N

Q

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T

S

N

D

N

G

I

V

A

C

T

A

F

V

C

L

L

D

S

G

A

P

S

A

G

A

H

V

G

G

G

M

M

E

E

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A

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T

L

L

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C

N

N

L

L

E

E

P

D

G

G

E

D

R

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L

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D

H

N

T

G

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H

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A

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A

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L

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A

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L

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I

A

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A

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L

A

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K

Y

P

H

S

A

V

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M

L

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T

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A

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E

D

S
|
S

S

S

T

T

G

G

V

V

R

L

S

Q

P

G

V

L

G

E

A

G

V

V

G

G

D

A

L

L

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S

H

M

Q

H

H

G

N

A

C

L

L

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L

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I

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T

A

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S

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M

A

D

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M

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F

M

M

A

D

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W

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E

A

I

D

D

A

A

L

M

F

Y

A

T

S

G

S

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H

Q

K
|
K

C

V

L

L

S

G

C

A

P

P

G

G

L

I

A

T

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P

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V

A

S

F

F

S

S

M

H

R

R

A

A

L

V

D

E

R

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V

Y

G

K

R

R

R

N

T

T

P

K

I

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P

K

G

V

R

Y

Y

Y

F

W

D

D

I

M

A

S

L

L

H

V

L

G

D

D

Y

Y

W

W

Q

G

-

C

-

F

G

G

A

R

P

P

R

R

V

I

C

Y

H

H

L

H

M

T

P

I

S

S

V

S

N

T

L

L

Y

Y

A

G

L

L

H

R

M

E

A

A

L

I

E

A

G

M

V

A

L

C

N

E

E

E

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G

L

L

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P

N

A

V

L

F

I

A

A

R

R

H

H

R

E

A

D

A

C

H

A

E

K

Q

R

L

L

V

Y

T

R

G

G

L

L

A

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A

D

L

A

G

G

L

F

R

E

P

L

L

Y

A

A

D

D

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P

P

K

W

D

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R

L

L

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M

T

L

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T

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I

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A

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P

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E

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G

G

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D

A

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E

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A

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A

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Y

G

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E

E

I

I

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G

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L

T

A

A

G

G

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Q

V

V

W

F

R
|
R

I

I

G

G

L

L

M

M

G

G

H

Q

S

N

A

A

R

T

T

T

T

E

N

R

V

V

V

D

A

R

L

V

L

L

E

Q

A

V

L

F

binding glyoxylic acid: P25 (≠ D23), S155 (= S153), K206 (= K204), R356 (= R352)

2hufA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase (see paper)
37% identity, 94% coverage: 19:372/376 of query aligns to 21:376/385 of 2hufA

query
sites
2hufA

L

L

M

M

G

G

D

P

G

G

P

P

S

S

G

N

V

A

P

P

D

Q

S

R

V

V

Y

L

S

D

A

A

L

M

A

S

L

R

P

P

T

I

V

L

G

G

P
x
H

L

L

D

H

P

P

G

E

F

T

D

L

E

K

I

I

L

M

D

D

R

D

L

I

Q

K

G

E

Q

G

L

V

R

R

N

Y

I

L

F

F

N

Q

V

T

S

N

D

N

G

I

V

A

C

T

A

F

V

C

L

L

D

S

G

A

P

S

A

G

A

H

V

G

G

G

M

M

E

E

T

A

C

T

L

L

V

C

N

N

L

L

E

E

P

D

G

G

E

D

R

V

L

I

L

L

V

I

L

G

D

H

N

T

G

G

M

H

G

W

G

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E

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L

S

R

A

E

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T

M

A

A

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R

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Y

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G

L

A

A

D

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V

D

R

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K

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K

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V

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G

D

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L

L

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S

L

E

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Q

E

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I

A

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Q

D

R

A

L

L

A

L

R

I

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H

D

K

Y

P

H

S

A

V

V

L

V

F

M

L

V

T

H

Q

A

G

E

D

S
|
S

S

S

T

T

G

G

V

V

R

L

S

Q

P

G

V

L

G

E

A

G

V

V

G

G

D

A

L

L

V

C

S

H

M

Q

H

H

G

N

A

C

L

L

F

L

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I

V

V

D

D

C

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E

V

T

A

S

S

L

L

G

G

M

A

D

P

V

M

D

F

M

M

A

D

R

R

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W

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E

A

I

D

D

A

A

L

M

F

Y

A

T

S

G

S

S

H

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K

K

C

V

L

L

S

G

C

A

P

P

G

G

L

I

A

T

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P

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V

A

S

F

F

S

S

M

H

R

R

A

A

L

V

D

E

R

R

V

Y

G

K

R

R

R

N

T

T

P

K

I

V

P

K

G

V

R

Y

Y

Y

F

W

D

D

I

M

A

S

L

L

H

V

L

G

D

D

Y

Y

W

W

Q

G

-

C

-

F

G

G

A

R

P

P

R

R

V

I

C
x
Y

H

H

L

H

M

T

P

I

S

S

V

S

N

T

L

L

Y

Y

A

G

L

L

H

R

M

E

A

A

L

I

E

A

G

M

V

A

L

C

N

E

E

E

G

G

L

L

P

P

N

A

V

L

F

I

A

A

R

R

H

H

R

E

A

D

A

C

H

A

E

K

Q

R

L

L

V

Y

T

R

G

G

L

L

A

Q

A

D

L

A

G

G

L

F

R

E

P

L

L

Y

A

A

D

D

V

P

P

K

W

D

R

R

L

L

P

S

M

T

L

V

H

T

V

T

V

I

P

K

A

V

P

P

E

Q

G

G

V

V

D

D

A

W

E

L

D

K

V

A

R

A

G

Q

R

Y

L

A

R

M

A

K

E

T

H

Y

G

L

I

V

E

E

I

I

G

S

G

G

V

L

G

G

R

P

L

T

A

A

G

G

R

Q

V

V

W

F

R
|
R

I

I

G

G

L

L

M

M

G

G

H

Q

S

N

A

A

R

T

T

T

T

E

N

R

V

V

V

D

A

R

L

V

L

L

E

Q

A

V

L

F

binding 1-butanol: H45 (≠ P43), S155 (= S153), Y257 (≠ C253), R356 (= R352)

Q3LSM4 Alanine--glyoxylate aminotransferase; EC 2.6.1.44 from Aedes aegypti (Yellowfever mosquito) (Culex aegypti) (see paper)
37% identity, 94% coverage: 19:372/376 of query aligns to 21:376/393 of Q3LSM4

query
sites
Q3LSM4

L

L

M

M

G

G

D

P

G

G

P

P

S

S

G

N

V

A

P

P

D

Q

S

R

V

V

Y

L

S

D

A

A

L

M

A

S

L

R

P

P

T

I

V

L

G

G

P

H

L

L

D

H

P

P

G

E

F

T

D

L

E

K

I

I

L

M

D

D

R

D

L

I

Q

K

G

E

Q

G

L

V

R

R

N

Y

I

L

F

F

N

Q

V

T

S

N

D

N

G

I

V

A

C

T

A

F

V

C

L

L

D

S

G

A

P
x
S

A
x
G

A
x
H

V

G

G

G

M

M

E

E

T

A

C

T

L

L

V

C

N

N

L

L

E

E

P

D

G

G

E

D

R

V

L

I

L

L

V

I

L

G

D

H

N

T

G

G

M

H

G

W

G

G

E

D

R

R

L

S

R

A

E

D

T

M

A

A

Q

T

R

R

L

Y

G

G

L

A

A

D

V

V

D

R

V

V

L

V

T

K

V

S

P

K

W

V

G

G

D

Q

P

S

V

L

L

S

S

L

E

D

Q

E

V

I

A

R

Q

D

R

A

L

L

A

L

R

I

S

H

D

K

Y

P

H

S

A

V

V

L

V

F

M

L

V

T

H

Q

A

G

E

D

S
|
S

S

S

T

T

G

G

V

V

R

L

S

Q

P

G

V

L

G

E

A

G

V

V

G

G

D

A

L

L

V

C

S

H

M

Q

H

H

G

N

A

C

L

L

F

L

I

I

V

V

D

D

C

T

E

V

T

A

S

S

L

L

G

G

M

A

D

P

V

M

D

F

M

M

A

D

R

R

W

W

R

E

A

I

D

D

A

A

L

M

F

Y

A

T

S

G

S

S

H
x
Q

K
|
K

C

V

L

L

S

G

C

A

P

P

G

G

L

I

A

T

P

P

V

V

A

S

F

F

S

S

M

H

R

R

A

A

L

V

D

E

R

R

V

Y

G

K

R

R

R

N

T

T

P

K

I

V

P

K

G

V

R

Y

Y

Y

F

W

D

D

I

M

A

S

L

L

H

V

L

G

D

D

Y

Y

W

W

Q

G

-

C

-

F

G

G

A

R

P

P

R

R

V

I

C
x
Y

H

H

L

H

M
x
T

P

I

S

S

V

S

N

T

L

L

Y

Y

A

G

L

L

H

R

M

E

A

A

L

I

E

A

G

M

V

A

L

C

N

E

E

E

G

G

L

L

P

P

N

A

V

L

F

I

A

A

R

R

H

H

R

E

A

D

A

C

H

A

E

K

Q

R

L

L

V

Y

T

R

G

G

L

L

A

Q

A

D

L

A

G

G

L

F

R

E

P

L

L

Y

A

A

D

D

V

P

P

K

W

D

R

R

L

L

P

S

M

T

L

V

H

T

V

T

V

I

P

K

A

V

P

P

E

Q

G

G

V

V

D

D

A

W

E

L

D

K

V

A

R

A

G

Q

R

Y

L

A

R

M

A

K

E

T

H

Y

G

L

I

V

E

E

I

I

G

S

G

G

V

L

G

G

R

P

L

T

A

A

G

G

R

Q

V

V

W

F

R
|
R

I

I

G

G

L

L

M

M

G

G

H

Q

S

N

A

A

R

T

T

T

T

E

N

R

V

V

V

D

A

R

L

V

L

L

E

Q

A

V

L

F

SGH 78:80 (≠ PAA 76:78) binding in other chain
S155 (= S153) binding glyoxylate; binding L-alanine
Q205 (≠ H203) binding in other chain
K206 (= K204) modified: N6-(pyridoxal phosphate)lysine
Y257 (≠ C253) binding pyridoxal 5'-phosphate
T260 (≠ M256) binding pyridoxal 5'-phosphate
R356 (= R352) binding glyoxylate

3kgwB Crystal structure of putative aminotransferase (aah25799.1) from mus musculus at 1.65 a resolution
34% identity, 97% coverage: 7:372/376 of query aligns to 17:378/388 of 3kgwB

query
sites
3kgwB

P

P

L

L

G

S

H

V

P

P

L

T

R

R

N

-

I

-

K

-

A

-

L

-

L

L

M

L

G

G

D

P

G

G

P

P

S

S

G

N

V

L

P

A

D

P

S

R

V

V

Y

L

S

A

A

A

L

G

A

S

L

L

P

R

T

M

V

I

G

G

P

H

L

M

D

Q

P

K

G

E

F

M

D

L

E

Q

I

I

L

M

D

E

R

E

L

I

Q

K

G

Q

Q

G

L

I

R

Q

N

Y

I

V

F

F

N

Q

V

T

S

R

D

N

G

P

V

L

C

T

A

L

V

V

L

V

D

S

G

G

P
x
S

A
x
G

A
x
H

V

C

G

A

M

M

E

E

T

T

C

A

L

L

V

F

N

N

L

L

E

E

P

P

G

G

E

D

R

S

L

F

L

L

V

T

L

G

D

T

N

N

G

G

M

I

G
x
W

G

G

E

M

R

R

L

A

R

A

E

E

T

I

A

A

Q

D

R

R

L

I

G

G

L

A

A

R

V

V

D

H

V

Q

L

M

T

I

V

K

P

K

W

P

G

G

D

E

P

H

V

Y

L

T

S

L

E

Q

Q

E

V

V

A

E

Q

E

R

G

L

L

A

A

R

Q

S

H

D

K

Y

P

H

V

A

L

V

L

V

F

M

L

V

V

H

H

A

G

E

E

S
|
S

S

S

T

T

G

G

V

V

R

V

S

Q

P

P

V

L

G

D

A

G

V

F

G

G

D

E

L

L

V

C

S

H

M

R

H

Y

G

Q

A

C

L

L

F

L

I

L

V

V

D
|
D

C

S

E
x
V

T

A

S

S

L

L

G

G

M

V

D

P

V

I

D

Y

M

M

A

D

R

Q

W

Q

R

G

A

I

D

D

A

I

L

M

F

Y

A

S

S

S

H

Q

K
|
K

C

V

L

L

S

N

C

A

P

P

G

G

L

I

A

S

P

L

V

I

A

S

F

F

S

N

M

D

R

K

A

A

L

K

D

Y

R

K

V

V

G

Y

R

S

R

R

R

K

T

T

P

K

I

P

P

V

G

S

R

F

Y

Y

F

T

D

D

I

I

A

T

L

Y

H

L

L

A

D

K

Y

L

W

W

Q

G

G

C

A

E

P

T

R

R

V

V

C

I

H

H

L

H

M

T

P

T

S

P

V

V

N

T

L

S

L

L

Y

Y

A

C

L

L

H

R

M

E

A

S

L

L

E

A

G

L

V

I

L

A

N

E

E

Q

G

G

L

L

P

E

N

N

V

C

F

W

A

R

R

R

H

H

R

R

A

E

A

A

H

T

E

A

Q

H

L

L

V

H

T

K

G

H

L

L

A

Q

A

E

L

M

G

G

L

L

R

K

P

F

L

F

A

V

D

K

V

D

P

P

-

E

W

I

R

R

L

L

P

P

M

T

L

I

H

T

V

T

V

V

P

T

A

V

P

P

E

A

G

G

V

Y

D

N

A

W

E

R

D

D

V

I

R

V

G

S

R

Y

L

V

R

L

A

D

E

H

H

F

G

S

I

I

E

E

I

I

G

S

G

G

V

L

G

G

R

P

L

T

A

E

G

E

R

R

V

V

W

L

R

R

I

I

G

G

L

L

M

L

G

G

H

Y

S

N

A

A

R

T

T

T

T

E

N

N

V

V

V

D

A

R

L

V

L

A

E

E

A

A

L

L

binding pyridoxal-5'-phosphate: S81 (≠ P76), G82 (≠ A77), H83 (≠ A78), W108 (≠ G103), S158 (= S153), D183 (= D178), V185 (≠ E180), K209 (= K204)

2yrrA Hypothetical alanine aminotransferase (tth0173) from thermus thermophilus hb8
40% identity, 95% coverage: 18:376/376 of query aligns to 1:350/352 of 2yrrA

query
sites
2yrrA

L

M

L

L

M

L

G

T

D

P

G

G

P

P

S

T

G

P

V

I

P

P

D

E

S

R

V

V

Y

Q

S

K

A

A

L

L

A

L

L

R

P

P

T

M

V

R

G

G

P

H

L

L

D

D

P

P

G

E

F

V

D

L

E

R

I

V

L

N

D

R

R

A

L

I

Q

Q

G

E

Q

R

L

L

R

A

N

A

I

L

F

F

N

D

V

P

S

G

D

E

G

G

-

A

V

L

C

V

A

A

V

A

L

L

D

A

G

G

P
x
S

A
x
G

A
x
S

V

L

G

G

M

M

E

E

T

A

C

G

L

L

V

A

N

N

L

L

L

-

E

-

P

-

G

-

E

D

R

R

-

G

-

P

L

V

L

L

V

V

L

L

D

V

N

N

G

G

M

A

G
x
F

G

S

E

Q

R

R

L

V

R

A

E

E

T

M

A

A

Q

A

R

L

L

H

G

G

L

L

A

D

V

P

D

E

V

V

L

L

T

D

V

F

P

P

W

P

G

G

D

E

P

P

V

V

L

D

S

P

E

E

Q

A

V

V

A

A

Q

R

R

A

L

L

A

K

R

R

S

R

D

R

Y

Y

H

R

A

M

V

V

V

A

M

L

V

V

H

H

A

G

E

E

S
x
T

S

S

T

T

G

G

V

V

R

L

S

N

P

P

V

A

G

E

A

A

V

I

G

G

D

A

L

L

V

A

S

K

M

E

H

A

G

G

A

A

L

L

F

F

I

F

V

L

D
|
D

C

A

E
x
V

T

T

S

T

L

L

G

G

G

M

M

L

D

P

V

F

D

S

M

M

A

R

R

A

W

M

R

G

A

V

D

D

A

Y

L

A

F

F

A

T

S

G

S

S

H

Q

K
|
K

C

C

L

L

S

S

C

A

P

P

G

G

L

L

A

A

P

P

V

I

A

A

F

A

S

S

M

L

R

E

A

A

L

R

D

K

R

A

V

F

G

T

R

G

R

K

R

R

T

-

P

-

I

-

P

-

G

G

R

W

Y

Y

F

L

D

D

I

L

A

A

L

R

H

V

L

A

D

E

Y

H

W

W

Q

E

G

R

A

G

P

G

R

-

V

-

C

Y

H

H

L

H

M

T

P

T

S

P

V

V

N

L

L

L

L

H

Y

Y

A

A

L

L

H

L

M

E

A

A

L

L

E

D

G

L

V

V

L

L

N

E

E

E

G

G

L

V

P

A

N

A

V

R

F

E

A

R

R

R

H

A

R

R

A

E

A

V

H

Y

E

A

Q

W

L

V

V

L

T

E

G

E

L

L

A

K

A

A

L

R

G

G

L

F

R

R

P

P

L

Y

A

P

D

K

V

A

P

S

W

-

R

P

L

L

P

P

M

T

L

V

H

L

V

V

P

R

A

P

P

P

E

E

G

G

V

V

D

D

A

A

E

D

D

R

V

L

R

V

G

R

R

A

L

L

R

Y

A

A

E

E

H

-

G

G

I

V

E

A

I

V

G

A

G

G

V

I

G

G

R

P

L

T

A

R

G

G

R

Q

V

V

W

L

R

R

I

L

G

G

L

L

M

M

G

G

H

E

S

G

A

A

R

R

T

R

T

E

N

A

V

Y

V

Q

A

A

L

F

L

L

E

K

A

A

L

L

G

D

R

R

L

A

L

L

binding pyridoxal-5'-phosphate: S60 (≠ P76), G61 (≠ A77), S62 (≠ A78), F85 (≠ G103), T135 (≠ S153), D160 (= D178), V162 (≠ E180), K186 (= K204)

2yriA Crystal structure of alanine-pyruvate aminotransferase with 2- methylserine
40% identity, 95% coverage: 18:376/376 of query aligns to 1:350/352 of 2yriA

query
sites
2yriA

L

M

L

L

M

L

G

T

D

P

G
|
G

P

P

S

T

G

P

V

I

P

P

D

E

S

R

V

V

Y

Q

S

K

A

A

L

L

A

L

L

R

P

P

T

M

V

R

G

G

P

H

L

L

D

D

P

P

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N

D

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N

A

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G

-

A

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C

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A

A

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A

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D

A

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G

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x
S

A
x
G

A
x
S

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L

G

G

M

M

E

E

T

A

C

G

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A

N

N

L

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-

E

-

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-

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-

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-

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-

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x
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x
T

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K

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x
Y

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x
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-

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R

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A

A

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A

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A

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A

L

L

binding (s,e)-3-hydroxy-2-((3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl)methyleneamino)-2-methylpropanoic acid: G7 (= G24), S60 (≠ P76), G61 (≠ A77), S62 (≠ A78), F85 (≠ G103), T135 (≠ S153), D160 (= D178), V162 (≠ E180), K186 (= K204), R326 (= R352)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: Y229 (≠ C253), T232 (≠ M256)

3kgxA Crystal structure of putative aminotransferase (aah25799.1) from mus musculus at 1.80 a resolution
34% identity, 97% coverage: 7:372/376 of query aligns to 13:373/383 of 3kgxA

query
sites
3kgxA

P

P

L

L

G

S

H

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P

P

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N

-

I

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K

-

A

-

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-

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G

G

D

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G

G

P

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N

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Y

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A

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N

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P

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x
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x
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binding magnesium ion: H290 (≠ Q290), H294 (≠ G294), D367 (≠ V366)

P21549 Alanine--glyoxylate aminotransferase; AGT; Serine--pyruvate aminotransferase; SPT; EC 2.6.1.44; EC 2.6.1.51 from Homo sapiens (Human) (see 24 papers)
34% identity, 95% coverage: 14:372/376 of query aligns to 19:380/392 of P21549

query
sites
P21549

N

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x
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K

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x
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I

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x
V

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x
I

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A

A

L

L

R36 (≠ S31) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177157
G41 (≠ L36) to E: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177168; to R: in HP1; associated in cis with L-11 and M-340; results in loss of protein stability; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; partial mitochondrial mistargeting; intraperoxisomal protein aggregation seen; dbSNP:rs121908523; to V: in HP1; reduced alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177168
G47 (= G42) to R: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity; reduced expression levels; reduced pyridoxal phosphate binding; reduced dimerization; reduced thermostability; increased propensity to aggregation; increased susceptibility to proteolytic degradation within the N-terminal region; mitochondrial mistargeting; exposure to pyridoxine can rescue the functionality by partially preventing aggregation and degradation and by redirecting all the protein to the peroxisome; dbSNP:rs180177173
G82 (≠ A77) to E: in HP1; abolishes alanine--glyoxylate aminotransferase activity by interfering with pyridoxal phosphate binding; dbSNP:rs121908522
W108 (≠ G103) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs180177197
A112 (≠ L107) to D: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs796052061
L150 (≠ A145) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177222
F152 (≠ V147) to I: in HP1; associated in cis with L-11 and M-340; results in protein destabilization; no loss of dimerization; decreased alanine--glyoxylate aminotransferase activity; loss of alanine--glyoxylate aminotransferase activity when associated with L-11 and M-340; mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908524
G156 (≠ A151) to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs121908530
S158 (= S153) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177225
G161 (= G156) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227; to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; loss of dimerization; dbSNP:rs180177227; to S: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227
L166 (≠ V161) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177230
G170 (= G165) to R: in HP1; associated in cis with L-11 and M-340; decrease in alanine--glyoxylate aminotransferase activity; loss of dimerization; partial loss of protein stability but protein stability increases in the presence of pyridoxal phosphate; causes protein aggregation; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; results in mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908529
C173 (≠ V168) to Y: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs180177231
D183 (= D178) to N: in HP1; loss of alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177236
S187 (= S182) to F: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization but improved dimerization in the presence of pyridoxal phosphate; decreased protein stability; dbSNP:rs180177238
I202 (≠ A197) to N: in HP1; uncertain significance; dbSNP:rs536352238
S205 (≠ A200) to P: in HP1; loss of alanine--glyoxylate aminotransferase activity; decreased protein stability; dbSNP:rs121908520
K209 (= K204) mutation to R: Affects pyridoxal phosphate binding; loss of alanine--glyoxylate aminotransferase activity.
S218 (≠ A213) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; no effect on protein stability; dbSNP:rs180177253
R233 (= R228) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908526; to H: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908527
I244 (= I239) to T: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity in vitro; no loss of dimerization; partial mitochondrial mistargeting; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; dbSNP:rs121908525
C253 (vs. gap) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177264
I279 (≠ V272) to T: in dbSNP:rs140992177
A280 (≠ L273) to V: in dbSNP:rs73106685
V326 (≠ A318) to I: in dbSNP:rs115057148
I340 (≠ R332) to M: in allele minor; associated in cis with L-11; no effect on alanine--glyoxylate aminotransferase activity in vitro; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with L-11; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and I-152; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and R-170; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and T-244; results in mitochondrial mistargeting when associated with L-11 and R-170; dbSNP:rs4426527

Sites not aligning to the query:

9 T → N: no loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs115014558
11 P → L: in allele minor; associated in cis with M-340; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with I-152 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with R-170 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with T-244 and M-340; causes mitochondrial mistargeting when associated with R-170 and M-340; dbSNP:rs34116584

5hhyA Structure of human alanine:glyoxylate aminotransferase major allele (agt-ma) showing x-ray induced reduction of plp internal aldimine to 4'-deoxy-piridoxine-phosphate (plr) (see paper)
34% identity, 95% coverage: 14:372/376 of query aligns to 14:375/385 of 5hhyA

query
sites
5hhyA

N

S

I

I

K

P

A

N

L

Q

L

L

M

L

G

G

D

P

G

G

P

P

S

S

G

N

V

L

P

P

D

P

S

R

V

I

Y

M

S

A

A

A

L

G

A

G

L

L

P

Q

T

M

V

I

G

G

P

S

L

M

D

S

P

K

G

D

F

M

D

Y

E

Q

I

I

L

M

D

D

R

E

L

I

Q

K

G

E

Q

G

L

I

R

Q

N

Y

I

V

F

F

N

Q

V

T

S

R

D

N

G

P

V

L

C

T

A

L

V

V

L

I

D

S

G

G

P
x
S

A
x
G

A
x
H

V

C

G

A

M

L

E

E

T

A

C

A

L

L

V

V

N

N

L

V

L

L

E

E

P

P

G

G

E

D

R

S

L

F

L

L

V

V

L

G

D

A

N

N

G

G

M

I

G
x
W

G

G

E

Q

R

R

L

A

R

V

E

D

T

I

A

G

Q

E

R

R

L

I

G

G

L

A

A

R

V

V

D

H

V

P

L

M

T

T

V

K

P

D

W

P

G

G

D

G

P

H

V

Y

L

T

S

L

E

Q

Q

E

V

V

A

E

Q

E

R

G

L

L

A

A

R

Q

S

H

D

K

Y

P

H

V

A

L

V

L

V

F

M

L

V

T

H

H

A

G

E

E

S
|
S

S

S

T

T

G

G

V

V

R

L

S

Q

P

P

V

L

G

D

A

G

V

F

G

G

D

E

L

L

V

C

S

H

M

R

H

Y

G

K

A

C

L

L

F

L

I

L

V

V

D
|
D