SitesBLAST
Comparing 208639 MicrobesOnline__882:208639 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1vjoA Crystal structure of alanine--glyoxylate aminotransferase (alr1004) from nostoc sp. At 1.70 a resolution (see paper)
39% identity, 100% coverage: 2:376/376 of query aligns to 8:376/377 of 1vjoA
3islA Crystal structure of ureidoglycine-glyoxylate aminotransferase (pucg) from bacillus subtilis
37% identity, 98% coverage: 8:376/376 of query aligns to 3:365/387 of 3islA
O32148 (S)-ureidoglycine--glyoxylate transaminase; UGXT; (S)-ureidoglycine--glyoxylate aminotransferase; Purine catabolism protein PucG; EC 2.6.1.112 from Bacillus subtilis (strain 168) (see paper)
35% identity, 98% coverage: 8:376/376 of query aligns to 7:387/416 of O32148
- Q37 (≠ P43) mutation to H: 5-fold decrease in transamination activity.
- K198 (= K204) modified: N6-(pyridoxal phosphate)lysine
- N264 (≠ C253) mutation to S: 9-fold decrease in transamination activity.; mutation to Y: Loss of transamination activity.
2huuA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase in complex with alanine (see paper)
37% identity, 94% coverage: 19:372/376 of query aligns to 21:376/385 of 2huuA
2huiA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase in complex with glyoxylic acid (see paper)
37% identity, 94% coverage: 19:372/376 of query aligns to 21:376/385 of 2huiA
2hufA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase (see paper)
37% identity, 94% coverage: 19:372/376 of query aligns to 21:376/385 of 2hufA
Q3LSM4 Alanine--glyoxylate aminotransferase; EC 2.6.1.44 from Aedes aegypti (Yellowfever mosquito) (Culex aegypti) (see paper)
37% identity, 94% coverage: 19:372/376 of query aligns to 21:376/393 of Q3LSM4
- SGH 78:80 (≠ PAA 76:78) binding in other chain
- S155 (= S153) binding ; binding
- Q205 (≠ H203) binding in other chain
- K206 (= K204) modified: N6-(pyridoxal phosphate)lysine
- Y257 (≠ C253) binding
- T260 (≠ M256) binding
- R356 (= R352) binding
3kgwB Crystal structure of putative aminotransferase (aah25799.1) from mus musculus at 1.65 a resolution
34% identity, 97% coverage: 7:372/376 of query aligns to 17:378/388 of 3kgwB
2yrrA Hypothetical alanine aminotransferase (tth0173) from thermus thermophilus hb8
40% identity, 95% coverage: 18:376/376 of query aligns to 1:350/352 of 2yrrA
2yriA Crystal structure of alanine-pyruvate aminotransferase with 2- methylserine
40% identity, 95% coverage: 18:376/376 of query aligns to 1:350/352 of 2yriA
- binding (s,e)-3-hydroxy-2-((3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl)methyleneamino)-2-methylpropanoic acid: G7 (= G24), S60 (≠ P76), G61 (≠ A77), S62 (≠ A78), F85 (≠ G103), T135 (≠ S153), D160 (= D178), V162 (≠ E180), K186 (= K204), R326 (= R352)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: Y229 (≠ C253), T232 (≠ M256)
3kgxA Crystal structure of putative aminotransferase (aah25799.1) from mus musculus at 1.80 a resolution
34% identity, 97% coverage: 7:372/376 of query aligns to 13:373/383 of 3kgxA
P21549 Alanine--glyoxylate aminotransferase; AGT; Serine--pyruvate aminotransferase; SPT; EC 2.6.1.44; EC 2.6.1.51 from Homo sapiens (Human) (see 24 papers)
34% identity, 95% coverage: 14:372/376 of query aligns to 19:380/392 of P21549
- R36 (≠ S31) to C: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177157
- G41 (≠ L36) to E: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177168; to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of protein stability; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; partial mitochondrial mistargeting; intraperoxisomal protein aggregation seen; dbSNP:rs121908523; to V: in HP1; reduced alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177168
- G47 (= G42) to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity; reduced expression levels; reduced pyridoxal phosphate binding; reduced dimerization; reduced thermostability; increased propensity to aggregation; increased susceptibility to proteolytic degradation within the N-terminal region; mitochondrial mistargeting; exposure to pyridoxine can rescue the functionality by partially preventing aggregation and degradation and by redirecting all the protein to the peroxisome; dbSNP:rs180177173
- G82 (≠ A77) to E: in HP1; abolishes alanine--glyoxylate aminotransferase activity by interfering with pyridoxal phosphate binding; dbSNP:rs121908522
- W108 (≠ G103) to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs180177197
- A112 (≠ L107) to D: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs796052061
- L150 (≠ A145) to P: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177222
- F152 (≠ V147) to I: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in protein destabilization; decreased alanine--glyoxylate aminotransferase activity; no loss of dimerization; mitochondrial mistargeting; dbSNP:rs121908524
- G156 (≠ A151) to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs121908530
- S158 (= S153) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177225
- G161 (= G156) to C: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227; to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; loss of dimerization; dbSNP:rs180177227; to S: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227
- L166 (≠ V161) to P: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177230
- G170 (= G165) to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in mitochondrial mistargeting; slight decrease in alanine--glyoxylate aminotransferase activity; loss of dimerization; partial loss of protein stability but protein stability increases in the presence of pyridoxal phosphate; causes protein aggregation; dbSNP:rs121908529
- C173 (≠ V168) to Y: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs180177231
- D183 (= D178) to N: in HP1; loss of alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177236
- S187 (= S182) to F: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization but improved dimerization in the presence of pyridoxal phosphate; decreased protein stability; dbSNP:rs180177238
- I202 (≠ A197) to N: in HP1; uncertain significance; dbSNP:rs536352238
- S205 (≠ A200) to P: in HP1; loss of alanine--glyoxylate aminotransferase activity; decreased protein stability; dbSNP:rs121908520
- K209 (= K204) mutation to R: Affects pyridoxal phosphate binding; loss of alanine--glyoxylate aminotransferase activity.
- S218 (≠ A213) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; no effect on protein stability; dbSNP:rs180177253
- R233 (= R228) to C: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908526; to H: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908527
- I244 (= I239) to T: in HP1; prevalent mutation in the Canary islands; when associated with L-11 and M-340 on the minor AGXT allele; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity; no loss of dimerization; partial mitochondrial mistargeting; dbSNP:rs121908525
- C253 (vs. gap) to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177264
- I279 (≠ V272) to T: in dbSNP:rs140992177
- A280 (≠ L273) to V: in dbSNP:rs73106685
- V326 (≠ A318) to I: in dbSNP:rs115057148
- I340 (≠ R332) to M: associated with hyperoxaluria; dbSNP:rs4426527
Sites not aligning to the query:
- 9 T → N: no loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs115014558
- 11 P → L: reduction of specific alanine--glyoxylate aminotransferase activity in vitro; causes mitochondrial mistargeting when associated with R-170; dbSNP:rs34116584
5hhyA Structure of human alanine:glyoxylate aminotransferase major allele (agt-ma) showing x-ray induced reduction of plp internal aldimine to 4'-deoxy-piridoxine-phosphate (plr) (see paper)
34% identity, 95% coverage: 14:372/376 of query aligns to 14:375/385 of 5hhyA
- binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: S76 (≠ P76), G77 (≠ A77), H78 (≠ A78), W103 (≠ G103), S153 (= S153), D178 (= D178), V180 (≠ E180), Q203 (≠ H203), K204 (= K204), Y255 (≠ C253), T258 (≠ M256)
6rv0A Human alanine:glyoxylate aminotransferase major allele (agt-ma); with pmp in the active site (see paper)
34% identity, 95% coverage: 14:372/376 of query aligns to 14:375/384 of 6rv0A
6mfbD Crystal structure of 3-hydroxykynurenine transaminase from aedes aegypti
32% identity, 95% coverage: 19:376/376 of query aligns to 20:380/386 of 6mfbD
- binding pyridoxal-5'-phosphate: S77 (≠ P76), A78 (= A77), H79 (≠ A78), W104 (≠ G103), S154 (= S153), D179 (= D178), V181 (≠ E180), Q204 (≠ H203), K205 (= K204), Y256 (≠ V252), T259 (≠ L255)
2ch2A Structure of the anopheles gambiae 3-hydroxykynurenine transaminase in complex with inhibitor (see paper)
32% identity, 97% coverage: 12:376/376 of query aligns to 8:378/387 of 2ch2A
- binding 4-(2-aminophenyl)-4-oxobutanoic acid: G23 (= G24), S41 (≠ G42), N42 (≠ P43), S152 (= S153), Y254 (≠ C253), Q342 (≠ G340), L345 (≠ V343), R354 (= R352)
- binding pyridoxal-5'-phosphate: S75 (≠ P76), A76 (= A77), H77 (≠ A78), W102 (≠ G103), S152 (= S153), D177 (= D178), V179 (≠ E180), K203 (= K204), Y254 (≠ C253), T257 (≠ M256)
2ch1A Structure of anopheles gambiae 3-hydroxykynurenine transaminase (see paper)
32% identity, 97% coverage: 12:376/376 of query aligns to 9:379/388 of 2ch1A
- binding pyridoxal-5'-phosphate: S76 (≠ P76), A77 (= A77), H78 (≠ A78), W103 (≠ G103), S153 (= S153), D178 (= D178), V180 (≠ E180), Q203 (≠ H203), K204 (= K204), Y255 (≠ C253), T258 (≠ M256)
Q7PRG3 3-hydroxykynurenine transaminase; AgHKT; Alanine--glyoxylate aminotransferase; EC 2.6.1.63; EC 2.6.1.44 from Anopheles gambiae (African malaria mosquito) (see paper)
32% identity, 97% coverage: 12:376/376 of query aligns to 10:380/396 of Q7PRG3
- SAH 77:79 (≠ PAA 76:78) binding in other chain
- S154 (= S153) binding in other chain
- Q204 (≠ H203) binding in other chain
- K205 (= K204) modified: N6-(pyridoxal phosphate)lysine
- Y256 (≠ C253) binding
- T259 (≠ M256) binding
Q0IG34 3-hydroxykynurenine transaminase; 3-hydroxykynurenine transaminase and alanine--glyoxylate aminotransferase; Ae-HKT/AGT; Alanine--glyoxylate aminotransferase; EC 2.6.1.63; EC 2.6.1.44 from Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
32% identity, 95% coverage: 19:376/376 of query aligns to 20:380/400 of Q0IG34
- SAH 77:79 (≠ PAA 76:78) binding in other chain
- S154 (= S153) binding in other chain
- Q204 (≠ H203) binding in other chain
- K205 (= K204) modified: N6-(pyridoxal phosphate)lysine
- Y256 (≠ V252) binding
- T259 (≠ L255) binding
1j04A Structural mechanism of enzyme mistargeting in hereditary kidney stone disease in vitro (see paper)
33% identity, 95% coverage: 14:372/376 of query aligns to 16:377/387 of 1j04A
Query Sequence
>208639 MicrobesOnline__882:208639
MRTQEGPLGHPLRNIKALLLMGDGPSGVPDSVYSALALPTVGPLDPGFDEILDRLQGQLR
NIFNVSDGVCAVLDGPAAVGMETCLVNLLEPGERLLVLDNGMGGERLRETAQRLGLAVDV
LTVPWGDPVLSEQVAQRLARSDYHAVVMVHAESSTGVRSPVGAVGDLVSMHGALFIVDCE
TSLGGMDVDMARWRADALFASSHKCLSCPPGLAPVAFSMRALDRVGRRRTPIPGRYFDIA
LHLDYWQGAPRVCHLMPSVNLLYALHMALEGVLNEGLPNVFARHRAAHEQLVTGLAALGL
RPLADVPWRLPMLHVVPAPEGVDAEDVRGRLRAEHGIEIGGGVGRLAGRVWRIGLMGHSA
RTTNVVALLEALGRLL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory