SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 208688 DVU3168 glutamate-1-semialdehyde-2,1-aminomutase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3bs8A Crystal structure of glutamate 1-semialdehyde aminotransferase complexed with pyridoxamine-5'-phosphate from bacillus subtilis (see paper)
55% identity, 99% coverage: 3:421/423 of query aligns to 5:423/430 of 3bs8A

query
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3bs8A

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active site: V22 (= V20), Y145 (= Y143), E207 (= E205), D240 (= D238), M243 (≠ I241), K268 (= K266), G401 (≠ A399)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S117 (= S115), G118 (= G116), T119 (= T117), Y145 (= Y143), H146 (= H144), G147 (= G145), E207 (= E205), N212 (= N210), D240 (= D238), V242 (= V240), M243 (≠ I241), K268 (= K266)

3fqaA Gabaculien complex of gabaculine resistant gsam version (see paper)
56% identity, 99% coverage: 4:421/423 of query aligns to 4:422/426 of 3fqaA

query
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3fqaA

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active site: V20 (= V20), Y143 (= Y143), D238 (= D238), I241 (= I241), K266 (= K266), A400 (= A399)
binding 3-aminobenzoic acid: S22 (= S22), R25 (= R25), W60 (= W60), Y143 (= Y143), S156 (= S156)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S115 (= S115), G116 (= G116), T117 (= T117), C120 (≠ T120), Y143 (= Y143), H144 (= H144), G145 (= G145), E205 (= E205), N210 (= N210), D238 (= D238), V240 (= V240), I241 (= I241), K266 (= K266), G297 (= G297), T298 (= T298)

2gsaA Crystal structure of glutamate-1-semialdehyde aminomutase (aminotransferase, wild-type form) (see paper)
56% identity, 99% coverage: 4:421/423 of query aligns to 5:423/427 of 2gsaA

query
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2gsaA

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L

A

A

P

P

S

S

P

Q

F

F

E

E

A
|
A

A

G

M

F

V

T

S

S

F

L

A

A

H

H

T

T

D

E

D

E

D

D

L

I

A

D

A

A

M

T

L

L

D

A

A

A

A

A

R

R

K

T

V

V

active site: V21 (= V20), Y144 (= Y143), E206 (= E205), D239 (= D238), M242 (≠ I241), K267 (= K266), A401 (= A399)
binding pyridoxal-5'-phosphate: G298 (= G297), T299 (= T298)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S116 (= S115), G117 (= G116), T118 (= T117), Y144 (= Y143), H145 (= H144), G146 (= G145), E206 (= E205), N211 (= N210), D239 (= D238), V241 (= V240), M242 (≠ I241), K267 (= K266)

3usfA Crystal structure of dava-4
55% identity, 99% coverage: 4:421/423 of query aligns to 5:423/427 of 3usfA

query
sites
3usfA

R

K

S

S

S

D

E

E

L

I

F

F

E

A

R

A

A

A

Q

Q

Q

K

L

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V
|
V

R

R

A

A

C

F

L

K

G

S

V

V

D

G

S

G

E

Q

P

P

L

I

F

V

I

F

A

D

R

R

A

V

A

K

G

D

S

A

R

Y

L

A

H

W

T

D

V

V

D

D

G

G

E

N

T

R

F

Y

I

I

D

D

F

Y

V

V

E

G

S

T

W
|
W

G

G

P

P

M

A

L

I

L

C

G

G

H

H

T

A

H

H

P

P

E

E

V

V

T

I

A

E

A

A

V

L

H

K

A

V

A

A

V

M

D

E

R

K

G

G

T

T

S

S

Y

F

G

G

A

A

P

P

C

C

E

A

D

L

E

E

V

N

V

V

L

L

A

A

A

E

K

M

V

V

V

N

D

D

A

A

L

V

P

P

G

S

V

I

D

E

M

M

V

V

R

R

M

F

V

V

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

T

C

M

M

S

A

A

V

L

L

R

R

L

I

A

M

R

R

G

A

Y

Y

T

T

G

G

R

R

T

D

K

K

L

I

V

I

K

K

F

F

V

E

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

A

A

D

D

P

M

F

F

L

L

A

V

S

K

A

A

G

G

S
|
S

G

G

V

V

A

A

T

T

L

L

S

G

I

L

P

P

G

S

T

S

P

P

G

G

V

V

P

P

E

K

S

K

T

T

V

T

R

A

D

N

T

T

L

L

L

T

A

T

P

P

Y

Y

N

N

D

D

L

L

A

E

A

A

V

V

K

K

D

A

L

L

F

F

A

A

L

E

H

N

G

P

K

G

D

E

I

I

A

A

A

G

I

V

I

I

V

L

E
|
E

A

P

V

I

A

V

G

G

N
|
N

M

S

G

G

L

F

V

I

P

V

P

P

K

D

A

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

E

E

L

I

C

T

D

L

Q

E

H

H

G

D

A

A

L

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

I
x
M

T

T

G

G

F

F

R

R

V

I

S

A

F

Y

G

G

G

G

A

V

Q

Q

Q

E

R

K

F

F

G

G

I

V

T

T

P

P

D

D

L

L

T

T

T

T

L

L

G

G

K
|
K

I

I

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

Y

Y

G

G

G

G

K

K

R

R

E

E

I

I

M

M

Q

Q

R

L

I

V

A

A

P

P

C

A

G

G

E

P

V

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

A

T

A

A

G

G

I

I

A

K

T

T

L

L

D

E

V

L

L

L

S

R

R

Q

-

P

S

G

D

T

Y

Y

A

E

G

Y

L

L

E

D

A

Q

R

I

V

T

A

K

A

R

F

L

V

S

K

D

E

G

L

L

E

L

A

A

I

I

L

A

K

Q

G

E

K

T

G

G

V

H

P

A

V

A

R

C

I

G

N

G

T

Q

L

V

A

S

S

G

M

M

F

F

T

G

V

F

F

F

F

F

T

T

N

E

D

G

P

P

V

V

T

H

D

N

F

Y

A

E

S

D

A

A

K

K

T

K

A

S

D

D

G

L

A

Q

L

K

Y

F

T

S

S

R

F

F

Y

H

K

R

Q

G

M

M

R

L

A

E

Q

Q

G

G

I

I

Y

Y

L

L

A

A

P

P

S

S

P

Q

F

F

E
|
E

A
|
A

A

G

M

F

V

T

S

S

F

L

A

A

H

H

T

T

D

E

D

E

D

D

L

I

A

D

A

A

M

T

L

L

D

A

A

A

A

A

R

R

K

T

V

V

active site: V21 (= V20), Y144 (= Y143), D239 (= D238), M242 (≠ I241), K267 (= K266), A401 (= A399)
binding (4s)-4,5-diaminopentanoic acid: S23 (= S22), V25 (= V24), W61 (= W60), S157 (= S156), N211 (= N210), M242 (≠ I241), K267 (= K266), E400 (= E398)
binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: S116 (= S115), G117 (= G116), T118 (= T117), Y144 (= Y143), H145 (= H144), G146 (= G145), E206 (= E205), N211 (= N210), D239 (= D238), V241 (= V240), M242 (≠ I241), K267 (= K266), G298 (= G297), T299 (= T298)

3fq7A Gabaculine complex of gsam (see paper)
55% identity, 99% coverage: 4:421/423 of query aligns to 5:423/427 of 3fq7A

query
sites
3fq7A

R

K

S

S

S

D

E

E

L

I

F

F

E

A

R

A

A

A

Q

Q

Q

K

L

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V
|
V

R

R

A

A

C

F

L

K

G

S

V

V

D

G

S

G

E

Q

P

P

L

I

F

V

I

F

A

D

R

R

A

V

A

K

G

D

S

A

R

Y

L

A

H

W

T

D

V

V

D

D

G

G

E

N

T

R

F

Y

I

I

D

D

F

Y

V

V

E

G

S

T

W
|
W

G

G

P

P

M

A

L

I

L

C

G

G

H

H

T

A

H

H

P

P

E

E

V

V

T

I

A

E

A

A

V

L

H

K

A

V

A

A

V

M

D

E

R

K

G

G

T

T

S

S

Y

F

G

G

A

A

P

P

C

C

E

A

D

L

E

E

V

N

V

V

L

L

A

A

A

E

K

M

V

V

V

N

D

D

A

A

L

V

P

P

G

S

V

I

D

E

M

M

V

V

R

R

M

F

V

V

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

T
x
C

M

M

S

A

A

V

L

L

R

R

L

I

A

M

R

R

G

A

Y

Y

T

T

G

G

R

R

T

D

K

K

L

I

V

I

K

K

F

F

V

E

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

A

A

D

D

P

M

F

F

L

L

A

V

S

K

A

A

G

G

S
|
S

G

G

V

V

A

A

T

T

L

L

S

G

I

L

P

P

G

S

T

S

P

P

G

G

V

V

P

P

E

K

S

K

T

T

V

T

R

A

D

N

T

T

L

L

L

T

A

T

P

P

Y

Y

N

N

D

D

L

L

A

E

A

A

V

V

K

K

D

A

L

L

F

F

A

A

L

E

H

N

G

P

K

G

D

E

I

I

A

A

A

G

I

V

I

I

V

L

E
|
E

A

P

V

I

A

V

G

G

N
|
N

M

S

G

G

L

F

V

I

P

V

P

P

K

D

A

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

E

E

L

I

C

T

D

L

Q

E

H

H

G

D

A

A

L

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

I
x
M

T

T

G

G

F

F

R

R

V

I

S

A

F

Y

G

G

G

G

A

V

Q

Q

Q

E

R

K

F

F

G

G

I

V

T

T

P

P

D

D

L

L

T

T

T

T

L

L

G

G

K
|
K

I

I

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

Y

Y

G

G

G

G

K

K

R

R

E

E

I

I

M

M

Q

Q

R

L

I

V

A

A

P

P

C

A

G

G

E

P

V

M

Y

Y

Q

Q

A
|
A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

A

T

A

A

G

G

I

I

A

K

T

T

L

L

D

E

V

L

L

L

S

R

R

Q

-

P

S

G

D

T

Y

Y

A

E

G

Y

L

L

E

D

A

Q

R

I

V

T

A

K

A

R

F

L

V

S

K

D

E

G

L

L

E

L

A

A

I

I

L

A

K

Q

G

E

K

T

G

G

V

H

P

A

V

A

R

C

I

G

N

G

T

Q

L

V

A

S

S

G

M

M

F

F

T

G

V

F

F

F

F

F

T

T

N

E

D

G

P

P

V

V

T

H

D

N

F

Y

A

E

S

D

A

A

K

K

T

K

A

S

D

D

G

L

A

Q

L

K

Y

F

T

S

S

R

F

F

Y

H

K

R

Q

G

M

M

R

L

A

E

Q

Q

G

G

I

I

Y

Y

L

L

A

A

P

P

S

S

P

Q

F

F

E
|
E

A
|
A

A

G

M

F

V

T

S

S

F

L

A

A

H

H

T

T

D

E

D

E

D

D

L

I

A

D

A

A

M

T

L

L

D

A

A

A

A

A

R

R

K

T

V

V

active site: V21 (= V20), Y144 (= Y143), D239 (= D238), M242 (≠ I241), K267 (= K266), A401 (= A399)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: S23 (= S22), V25 (= V24), W61 (= W60), S116 (= S115), G117 (= G116), T118 (= T117), C121 (≠ T120), Y144 (= Y143), H145 (= H144), G146 (= G145), S157 (= S156), E206 (= E205), N211 (= N210), D239 (= D238), V241 (= V240), M242 (≠ I241), K267 (= K266), A297 (= A296), G298 (= G297), T299 (= T298), E400 (= E398)

2hp2A Inter-subunit signaling in gsam (see paper)
55% identity, 99% coverage: 4:421/423 of query aligns to 5:423/427 of 2hp2A

query
sites
2hp2A

R

K

S

S

S

D

E

E

L

I

F

F

E

A

R

A

A

A

Q

Q

Q

K

L

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V
|
V

R

R

A

A

C

F

L

K

G

S

V

V

D

G

S

G

E

Q

P

P

L

I

F

V

I

F

A

D

R

R

A

V

A

K

G

D

S

A

R

Y

L

A

H

W

T

D

V

V

D

D

G

G

E

N

T

R

F

Y

I

I

D

D

F

Y

V

V

E

G

S

T

W
|
W

G

G

P

P

M

A

L

I

L

C

G

G

H

H

T

A

H

H

P

P

E

E

V

V

T

I

A

E

A

A

V

L

H

K

A

V

A

A

V

M

D

E

R

K

G

G

T

T

S

S

Y

F

G

G

A

A

P

P

C

C

E

A

D

L

E

E

V

N

V

V

L

L

A

A

A

E

K

M

V

V

V

N

D

D

A

A

L

V

P

P

G

S

V

I

D

E

M

M

V

V

R

R

M

F

V

V

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

T
x
C

M

M

S

A

A

V

L

L

R

R

L

I

A

M

R

R

G

A

Y

Y

T

T

G

G

R

R

T

D

K

K

L

I

V

I

K

K

F

F

V

E

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

A

A

D

D

P

M

F

F

L

L

A

V

S

K

A

A

G

G

S

S

G

G

V

V

A

A

T

T

L

L

S

G

I

L

P

P

G

S

T

S

P

P

G

G

V

V

P

P

E

K

S

K

T

T

V

T

R

A

D

N

T

T

L

L

L

T

A

T

P

P

Y

Y

N

N

D

D

L

L

A

E

A

A

V

V

K

K

D

A

L

L

F

F

A

A

L

E

H

N

G

P

K

G

D

E

I

I

A

A

A

G

I

V

I

I

V

L

E
|
E

A

P

V

I

A

V

G

G

N
|
N

M

S

G

G

L

F

V

I

P

V

P

P

K

D

A

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

E

E

L

I

C

T

D

L

Q

E

H

H

G

D

A

A

L

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

I
x
M

T

T

G

G

F

F

R

R

V

I

S

A

F

Y

G

G

G

G

A

V

Q

Q

Q

E

R

K

F

F

G

G

I

V

T

T

P

P

D

D

L

L

T

T

T

T

L

L

G

G

K
|
K

I

I

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

Y

Y

G

G

G

G

K

K

R

R

E

E

I

I

M

M

Q

Q

R

L

I

V

A

A

P

P

C

A

G

G

E

P

V

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

A

T

A

A

G

G

I

I

A

K

T

T

L

L

D

E

V

L

L

L

S

R

R

Q

-

P

S

G

D

T

Y

Y

A

E

G

Y

L

L

E

D

A

Q

R

I

V

T

A

K

A

R

F

L

V

S

K

D

E

G

L

L

E

L

A

A

I

I

L

A

K

Q

G

E

K

T

G

G

V

H

P

A

V

A

R

C

I

G

N

G

T

Q

L

V

A

S

S

G

M

M

F

F

T

G

V

F

F

F

F

F

T

T

N

E

D

G

P

P

V

V

T

H

D

N

F

Y

A

E

S

D

A

A

K

K

T

K

A

S

D

D

G

L

A

Q

L

K

Y

F

T

S

S

R

F

F

Y

H

K

R

Q

G

M

M

R

L

A

E

Q

Q

G

G

I

I

Y

Y

L

L

A

A

P

P

S

S

P

Q

F

F

E
|
E

A
|
A

A

G

M

F

V

T

S

S

F

L

A

A

H

H

T

T

D

E

D

E

D

D

L

I

A

D

A

A

M

T

L

L

D

A

A

A

A

A

R

R

K

T

V

V

active site: V21 (= V20), Y144 (= Y143), D239 (= D238), M242 (≠ I241), K267 (= K266), A401 (= A399)
binding (4s)-4,5-diaminopentanoic acid: G298 (= G297), T299 (= T298)
binding (4r)-5-amino-4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: S23 (= S22), V25 (= V24), W61 (= W60), S116 (= S115), G117 (= G116), T118 (= T117), C121 (≠ T120), Y144 (= Y143), H145 (= H144), G146 (= G145), E206 (= E205), N211 (= N210), D239 (= D238), V241 (= V240), M242 (≠ I241), K267 (= K266), E400 (= E398)
binding pyridoxal-5'-phosphate: G298 (= G297), T299 (= T298)

2hp1A Inter-subunit signaling in gsam (see paper)
55% identity, 99% coverage: 4:421/423 of query aligns to 5:423/427 of 2hp1A

query
sites
2hp1A

R

K

S

S

S

D

E

E

L

I

F

F

E

A

R

A

A

A

Q

Q

Q

K

L

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V
|
V

R

R

A

A

C

F

L

K

G

S

V

V

D

G

S

G

E

Q

P

P

L

I

F

V

I

F

A

D

R

R

A

V

A

K

G

D

S

A

R

Y

L

A

H

W

T

D

V

V

D

D

G

G

E

N

T

R

F

Y

I

I

D

D

F

Y

V

V

E

G

S

T

W
|
W

G

G

P

P

M

A

L

I

L

C

G

G

H

H

T

A

H

H

P

P

E

E

V

V

T

I

A

E

A

A

V

L

H

K

A

V

A

A

V

M

D

E

R

K

G

G

T

T

S

S

Y

F

G
|
G

A

A

P

P

C

C

E

A

D

L

E

E

V

N

V

V

L

L

A

A

A

E

K

M

V

V

V

N

D

D

A

A

L

V

P

P

G

S

V

I

D

E

M

M

V

V

R

R

M

F

V

V

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

T
x
C

M

M

S

A

A

V

L

L

R

R

L

I

A

M

R

R

G

A

Y

Y

T

T

G

G

R

R

T

D

K

K

L

I

V

I

K

K

F

F

V

E

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

A

A

D

D

P

M

F

F

L

L

A

V

S

K

A

A

G

G

S

S

G

G

V

V

A

A

T

T

L

L

S

G

I

L

P

P

G

S

T

S

P

P

G

G

V

V

P

P

E

K

S

K

T

T

V

T

R

A

D

N

T

T

L

L

L

T

A

T

P

P

Y

Y

N

N

D

D

L

L

A

E

A

A

V

V

K

K

D

A

L

L

F

F

A

A

L

E

H

N

G

P

K

G

D

E

I

I

A

A

A

G

I

V

I

I

V

L

E
|
E

A

P

V

I

A

V

G

G

N
|
N

M

S

G

G

L

F

V

I

P

V

P

P

K

D

A

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

E

E

L

I

C

T

D

L

Q

E

H

H

G

D

A

A

L

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

I
x
M

T

T

G

G

F

F

R

R

V

I

S

A

F

Y

G

G

G

G

A

V

Q

Q

Q

E

R

K

F

F

G

G

I

V

T

T

P

P

D

D

L

L

T

T

T

T

L

L

G

G

K
|
K

I

I

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

Y

Y

G

G

G

G

K

K

R

R

E

E

I

I

M

M

Q

Q

R

L

I

V

A

A

P

P

C

A

G

G

E

P

V

M

Y

Y

Q

Q

A
|
A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

A

T

A

A

G

G

I

I

A

K

T

T

L

L

D

E

V

L

L

L

S

R

R

Q

-

P

S

G

D

T

Y

Y

A

E

G

Y

L

L

E

D

A

Q

R

I

V

T

A

K

A

R

F

L

V

S

K

D

E

G

L

L

E

L

A

A

I

I

L

A

K

Q

G

E

K

T

G

G

V

H

P

A

V

A

R

C

I

G

N

G

T

Q

L

V

A

S

S

G

M

M

F

F

T

G

V

F

F

F

F

F

T

T

N

E

D

G

P

P

V

V

T

H

D

N

F

Y

A

E

S

D

A

A

K

K

T

K

A

S

D

D

G

L

A

Q

L

K

Y

F

T

S

S

R

F

F

Y

H

K

R

Q

G

M

M

R

L

A

E

Q

Q

G

G

I

I

Y

Y

L

L

A

A

P

P

S

S

P

Q

F

F

E
|
E

A
|
A

A

G

M

F

V

T

S

S

F

L

A

A

H

H

T

T

D

E

D

E

D

D

L

I

A

D

A

A

M

T

L

L

D

A

A

A

A

A

R

R

K

T

V

V

active site: V21 (= V20), Y144 (= Y143), D239 (= D238), M242 (≠ I241), K267 (= K266), A401 (= A399)
binding (4s)-4-amino-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: S23 (= S22), V25 (= V24), W61 (= W60), S116 (= S115), G117 (= G116), T118 (= T117), C121 (≠ T120), Y144 (= Y143), H145 (= H144), G146 (= G145), E206 (= E205), N211 (= N210), D239 (= D238), V241 (= V240), M242 (≠ I241), K267 (= K266), E400 (= E398)
binding (4s)-4,5-diaminopentanoic acid: G88 (= G87), A297 (= A296), G298 (= G297), T299 (= T298)
binding pyridoxal-5'-phosphate: G298 (= G297), T299 (= T298)

2hozA Inter-subunit signaling in gsam (see paper)
55% identity, 99% coverage: 4:421/423 of query aligns to 5:423/427 of 2hozA

query
sites
2hozA

R

K

S

S

S

D

E

E

L

I

F

F

E

A

R

A

A

A

Q

Q

Q

K

L

L

I

M

P

P

G

G

G

G

V
|
V

N

S

S

S

P

P

V

V

R

R

A

A

C

F

L

K

G

S

V

V

D

G

S

G

E

Q

P

P

L

I

F

V

I

F

A

D

R

R

A

V

A

K

G

D

S

A

R

Y

L

A

H

W

T

D

V

V

D

D

G

G

E

N

T

R

F

Y

I

I

D

D

F

Y

V

V

E

G

S

T

W

W

G

G

P

P

M

A

L

I

L

C

G

G

H

H

T

A

H

H

P

P

E

E

V

V

T

I

A

E

A

A

V

L

H

K

A

V

A

A

V

M

D

E

R

K

G

G

T

T

S

S

Y

F

G

G

A

A

P

P

C

C

E

A

D

L

E

E

V

N

V

V

L

L

A

A

A

E

K

M

V

V

V

N

D

D

A

A

L

V

P

P

G

S

V

I

D

E

M

M

V

V

R

R

M

F

V

V

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

T
x
C

M

M

S

A

A

V

L

L

R

R

L

I

A

M

R

R

G

A

Y

Y

T

T

G

G

R

R

T

D

K

K

L

I

V

I

K

K

F

F

V
x
E

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

A

A

D

D

P

M

F

F

L

L

A

V

S

K

A

A

G
|
G

S
|
S

G

G

V

V

A

A

T

T

L

L

S

G

I

L

P

P

G

S

T

S

P

P

G

G

V

V

P

P

E

K

S

K

T

T

V

T

R

A

D

N

T

T

L

L

L

T

A

T

P
|
P

Y

Y

N

N

D

D

L

L

A

E

A

A

V

V

K

K

D

A

L

L

F

F

A

A

L

E

H

N

G

P

K

G

D

E

I

I

A

A

A

G

I

V

I

I

V

L

E
|
E

A

P

V

I

A

V

G

G

N
|
N

M

S

G

G

L

F

V

I

P

V

P

P

K

D

A

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

E

E

L

I

C

T

D

L

Q

E

H

H

G

D

A

A

L

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

I
x
M

T

T

G

G

F

F

R

R

V

I

S

A

F

Y

G

G

G

G

A

V

Q

Q

Q

E

R

K

F

F

G

G

I

V

T

T

P

P

D

D

L

L

T

T

T

T

L

L

G

G

K
|
K

I

I

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

Y

Y

G

G

G

G

K

K

R

R

E

E

I

I

M

M

Q

Q

R

L

I

V

A

A

P

P

C

A

G

G

E

P

V

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

A

T

A

A

G

G

I

I

A

K

T

T

L

L

D

E

V

L

L

L

S

R

R

Q

-

P

S

G

D

T

Y

Y

A

E

G

Y

L

L

E

D

A

Q

R

I

V

T

A

K

A

R

F

L

V

S

K

D

E

G

L

L

E

L

A

A

I

I

L

A

K

Q

G

E

K

T

G

G

V

H

P

A

V

A

R

C

I

G

N

G

T

Q

L

V

A

S

S

G

M

M

F

F

T

G

V

F

F

F

F

F

T

T

N

E

D

G

P

P

V

V

T

H

D
x
N

F

Y

A
x
E

S

D

A

A

K
|
K

T

K

A

S

D

D

G

L

A

Q

L

K

Y

F

T

S

S

R

F

F

Y

H

K

R

Q

G

M

M

R

L

A

E

Q

Q

G

G

I

I

Y

Y

L

L

A

A

P

P

S

S

P

Q

F

F

E

E

A
|
A

A

G

M

F

V

T

S

S

F

L

A

A

H

H

T

T

D

E

D

E

D

D

L

I

A

D

A

A

M

T

L

L

D

A

A

A

A

A

R

R

K

T

V

V

active site: V21 (= V20), Y144 (= Y143), D239 (= D238), M242 (≠ I241), K267 (= K266), A401 (= A399)
binding (4s)-4,5-diaminopentanoic acid: E141 (≠ V140), G156 (= G155), S157 (= S156), P182 (= P181), N368 (≠ D366), E370 (≠ A368), K373 (= K371)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S116 (= S115), G117 (= G116), T118 (= T117), C121 (≠ T120), Y144 (= Y143), H145 (= H144), G146 (= G145), E206 (= E205), N211 (= N210), D239 (= D238), V241 (= V240), M242 (≠ I241), K267 (= K266), G298 (= G297), T299 (= T298)

Q42522 Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic; GSA 2; Glutamate-1-semialdehyde aminotransferase 2; GSA-AT 2; EC 5.4.3.8 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
54% identity, 99% coverage: 3:421/423 of query aligns to 49:468/472 of Q42522

query
sites
Q42522

Q

Q

R

K

S

S

S

E

E

E

L

A

F

F

E

N

R

A

A

A

Q

K

Q

N

L

L

I

M

P

P

G

G

G

G

V

V

N

N

S

S

P

P

V

V

R

R

A

A

C

F

L

K

G

S

V

V

D

G

S

G

E

Q

P

P

L

V

F

V

I

M

A

D

R

S

A

A

A

K

G

G

S

S

R

R

L

I

H
x
R

T

D

V

I

D

D

G

G

E

N

T

E

F

Y

I

I

D

D

F

Y

V

V

E

G

S

S

W

W

G

G

P

P

M

A

L

I

L

I

G

G

H

H

T

A

H

D

P

D

E

E

V

V

T

L

A

A

A

A

V

L

H

A

A

E

A

T

V

M

D

K

R

K

G

G

T

T

S

S

Y

F

G

G

A

A

P

P

C

C

E

L

D

L

E

E

V

N

V

V

L

L

A

A

A

E

K

M

V

V

V

I

D

S

A

A

L

V

P

P

G

S

V

I

D

E

M

M

V

V

R

R

M

F

V

V

N

N

S

S

G
|
G

T

T

E

E

A

A

T

C

M

M

S

G

A

V

L

L

R

R

L

L

A

A

R

R

G

A

Y

F

T

T

G

G

R

K

T

Q

K

K

L

F

V

I

K

K

F

F

V

E

G

G

C

C

Y

Y

H

H

G

G

H

H

A

A

D

N

P

S

F

F

L

L

A

V

S

K

A

A

G

G

S

S

G

G

V

V

A

A

T

T

L

L

S

G

I

L

P

P

G

D

T

S

P

P

G

G

V

V

P

P

E

K

S

A

T

A

V

T

R

S

D

D

T

T

L

L

L

T

A

A

P

P

Y

Y

N

N

D

D

L

I

A

A

A

A

V

V

K

E

D

K

L

L

F

F

A

E

L

A

H

N

G

K

K

G

D

E

I

I

A

A

A

A

I

I

I

I

V

L

E

E

A

P

V

V

A

V

G

G

N

N

M

S

G

G

L

F

V

I

P

T

P

P

K

K

A

P

G

E

F

F

L

I

E

E

G

G

L

I

R

R

E

R

L

I

C

T

D

K

Q

D

H

N

G

G

A

A

L

L

L

L

I

I

F

F

D

D

E

E

V

V

I

M

T

T

G

G

F

F

R

R

V

L

S

A

F

Y

G

G

G

G

A

A

Q

Q

Q

E

R

Y

F

F

G

G

I

I

T

T

P

P

D

D

L

L

T

T

T

T

L

L

G

G

K

K

I

I

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

Y

Y

G

G

G

G

K

R

R

R

E

D

I

I

M

M

Q

E

R

M

I

V

A

A

P

P

C

A

G

G

E

P

V

M

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

A

T

A

A

G

G

I

I

A

H

T

T

L

L

D

K

V

R

L

L

S

S

R

Q

-

P

S

G

D

T

Y

Y

A

E

G

Y

L

L

E

D

A

K

R

I

V

T

A

K

A

E

F

L

V

T

K

N

E

G

L

I

E

L

A

E

I

A

L

G

K

K

G

K

K

T

G

G

V

H

P

A

V

M

R

C

I

G

N

G

T

Y

L

I

A

S

S

G

M

M

F

F

T

G

V

F

F

F

F

F

T

T

N

E

D

G

P

P

V

V

T

Y

D

D

F

F

A

S

S

D

A

A

K

K

T

K

A

S

D

D

G

T

A

E

L

K

Y

F

T

G

S

K

F

F

Y

F

K

R

Q

G

M

M

R

L

A

E

Q

E

G

G

I

V

Y

Y

L

L

A

A

P

P

S

S

P

Q

F

F

E

E

A

A

A

G

M

F

V

T

S

S

F

L

A

A

H

H

T

T

D

S

D

E

D

D

L

I

A

Q

A

F

M

T

L

I

D

A

A

A

A

A

R

E

K

K

V

V

R92 (≠ H46) mutation to K: In gsa2-1; suppression of enf1 mutant pleiotropic developmental phenotypes; when associated with S-162.
G162 (= G116) mutation to S: In gsa2-1; suppression of enf1 mutant pleiotropic developmental phenotypes; when associated with K-92.

3k28A Crystal structure of a glutamate-1-semialdehyde aminotransferase from bacillus anthracis with bound pyridoxal 5'phosphate
54% identity, 96% coverage: 4:410/423 of query aligns to 6:407/423 of 3k28A

query
sites
3k28A

R

K

S

S

S

I

E

A

L

A

F

F

E

E

R

E

A

A

Q

Q

Q

D

L

L

I

M

P

P

G

G

G

G

V
|
V

N

N

S

S

P

P

V

V

R

R

A

A

C

F

L

K

G

S

V

V

D

G

S

M

E

N

P

P

L

L

F

F

I

M

A

E

R

R

A

G

A

K

G

G

S

S

R

K

L

V

H

Y

T

D

V

I

D

D

G

G

E

N

T

E

F

Y

I

I

D

D

F

Y

V

V

E

L

S

S

W

W

G

G

P

P

M

L

L

I

L

H

G

G

H

H

T

A

H

N

P

D

E

R

V

V

T

V

A

E

A

A

V

L

H

K

A

A

A

V

V

A

D

E

R

R

G

G

T

T

S

S

Y

F

G

G

A

A

P

P

C

T

E

E

D

I

E

E

V

N

V

K

L

L

A

A

A

K

K

L

V

V

V
x
I

D

E

A

R

L
x
V

P
|
P

G

S

V
x
I

D

E

M

I

V

V

R

R

M

M

V

V

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

T

T

M

M

S

S

A

A

L

L

R

R

L

L

A

A

R

R

G

G

Y

Y

T

T

G

G

R

R

T

N

K

K

L

I

V

L

K

K

F

F

V

I

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

A

G

D

D

P

S

F

L

L

L

A

I

S

K

A

A

G

G

S

S

G

G

V

V

A

-

T

-

L

-

S

-

I

-

P

P

G

D

T

S

P

P

G

G

V

V

P

P

E

E

S

G

T

V

V

A

R

K

D

N

T

T

L

I

L

T

A

V

P

A

Y

Y

N

N

D

D

L

L

A

E

A

S

V

V

K

K

D

Y

L

A

F

F

A

E

L

Q

H

F

G

G

K

D

D

D

I

I

A

A

A

C

I

V

I

I

V

V

E
|
E

A

P

V

V

A

A

G

G

N

N

M

M

G

G

L

V

V

V

P

P

P

P

K

Q

A

P

G

G

F

F

L

L

E

E

G

G

L

L

R

R

E

E

L

V

C

T

D

E

Q

Q

H

N

G

G

A

A

L

L

L

L

I

I

F

F

D
|
D

E

E

V
|
V

I
x
M

T

T

G

G

F

F

R

R

V

V

S

A

F

Y

G

N

G

C

A

G

Q

Q

Q

G

R

Y

F

Y

G

G

I

V

T

T

P

P

D

D

L

L

T

T

T

C

L

L

G

G

K
|
K

I

V

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

Y

Y

G

G

G

G

K

K

R

A

E

E

I

I

M

M

Q

R

R

Q

I

V

A

A

P

P

C

S

G

G

E

P

V

I

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

A

A

A

A

G

G

I

Y

A

E

T

T

L

L

D

V

V

Q

L

L

S

T

R

P

S

E

D

S

Y

Y

A

V

G

E

L

F

E

E

A

R

R

K

V

A

A

E

A

M

F

L

V

E

K

A

E

G

L

L

E

R

A

K

I

A

L

A

K

E

G

K

K

H

G

G

V

I

P

P

V

H

R

H

I

I

N

N

T

R

L

A

A

G

S

S

M

M

F

I

T

G

V

I

F

F

F

F

T

T

N

D

D

E

P

P

V

V

T

I

D

N

F

Y

A

D

S

A

A

A

K

K

T

S

A

S

D

N

G

L

A

Q

L

F

Y

F

T

A

S

A

F

Y

Y

Y

K

R

Q

E

M

M

R

V

A

E

Q

Q

G

G

I

V

Y

F

L

L

A

P

P

P

S

S

P

Q

F

F

E

E

A
x
G

A

L

M

F

V

L

S

S

F

T

A

V

H

H

T

S

D

D

D

A

D

D

active site: V22 (= V20), Y145 (= Y143), E202 (= E205), D235 (= D238), M238 (≠ I241), K263 (= K266), G396 (≠ A399)
binding calcium ion: I103 (≠ V101), V106 (≠ L104), P107 (= P105), I109 (≠ V107)
binding pyridoxal-5'-phosphate: S117 (= S115), G118 (= G116), T119 (= T117), Y145 (= Y143), H146 (= H144), G147 (= G145), E202 (= E205), D235 (= D238), V237 (= V240), M238 (≠ I241), K263 (= K266), G294 (= G297), T295 (= T298)

P42799 Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic; AtGSA1; GSA 1; Glutamate-1-semialdehyde aminotransferase 1; GSA-AT 1; EC 5.4.3.8 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
53% identity, 99% coverage: 3:421/423 of query aligns to 51:470/474 of P42799

query
sites
P42799

Q

Q

R

K

S

S

S

E

E

E

L

A

F

F

E

N

R

A

A

A

Q

K

Q

N

L

L

I

M

P

P

G

G

G

G

V

V

N

N

S

S

P

P

V

V

R

R

A

A

C

F

L

K

G

S

V

V

D

G

S

G

E

Q

P

P

L

V

F

L

I

I

A

D

R

S

A

V

A

K

G

G

S

S

R

K

L

M

H

W

T

D

V

I

D

D

G

G

E

N

T

E

F

Y

I

I

D

D

F

Y

V

V

E

G

S

S

W

W

G

G

P

P

M

A

L

I

L

I

G

G

H

H

T

A

H

D

P

D

E

E

V

V

T

L

A

A

A

A

V

L

H

A

A

E

A

T

V

M

D

K

R

K

G

G

T

T

S

S

Y

F

G

G

A

A

P

P

C

C

E

L

D

L

E

E

V

N

V

V

L

L

A

A

A

E

K

M

V

V

V

I

D

S

A

A

L

V

P

P