SitesBLAST

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
51% identity, 97% coverage: 14:446/447 of query aligns to 8:446/446 of A0R083

query
sites
A0R083

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K363 (≠ N363) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
49% identity, 97% coverage: 12:444/447 of query aligns to 6:440/443 of 4lnkA

query
sites
4lnkA

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active site: D52 (= D58), E131 (= E136), E133 (= E138), E188 (= E189), E195 (= E196), H244 (= H245), R315 (= R316), E332 (= E336), R334 (= R338)
binding adenosine-5'-diphosphate: K43 (≠ A49), M50 (≠ G56), N127 (≠ Y132), G129 (= G134), E131 (= E136), F198 (≠ L199), K199 (≠ R200), Y200 (= Y201), N246 (≠ H247), L247 (≠ Q248), S248 (= S249), F250 (= F251), S324 (≠ G328), I327 (≠ A331), S328 (≠ A332), R330 (= R334)
binding glutamic acid: E133 (= E138), Y155 (= Y156), E188 (= E189), V189 (= V190), N239 (= N240), G240 (= G241), G242 (= G243), H244 (= H245), R297 (= R298), E303 (= E304), R334 (= R338)
binding magnesium ion: E131 (= E136), E188 (= E189), E195 (= E196), H244 (= H245), E332 (= E336)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
49% identity, 97% coverage: 12:444/447 of query aligns to 6:440/443 of 4lniA

query
sites
4lniA

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E

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D

E

L

R

G

M

K

E

H

N

G

G

I

I

G

V

S

D

L

L

P

P

G

A

S

T

L

L

Y

A

E

E

A

A

A

L

M

E

E

E

L

F

R

K

N

S

S

N

N

E

L

V

M

M

K

V

E

K

I

A

L

L

G

G

E

E

H

H

T

L

H

F

A

E

N

H

I

F

V

I

G

E

N

A

K

K

L

E

I

I

E

E

W

W

D

D

D

M

Y

F

R

R

T

T

H

Q

I

V

S

H

E

P

F

W

E

E

L

R

K

E

R

Q

Y

Y

L

M

active site: D52 (= D58), E131 (= E136), E133 (= E138), E188 (= E189), E195 (= E196), H244 (= H245), R315 (= R316), E332 (= E336), R334 (= R338)
binding adenosine-5'-diphosphate: N127 (≠ Y132), G129 (= G134), E131 (= E136), E183 (= E184), D197 (= D198), F198 (≠ L199), K199 (≠ R200), Y200 (= Y201), N246 (≠ H247), S248 (= S249), R315 (= R316), R320 (= R321), I327 (≠ A331), S328 (≠ A332), R330 (= R334), E332 (= E336)
binding magnesium ion: E131 (= E136), E131 (= E136), E133 (= E138), E188 (= E189), E195 (= E196), E195 (= E196), H244 (= H245), E332 (= E336)
binding l-methionine-s-sulfoximine phosphate: E131 (= E136), E133 (= E138), Y155 (= Y156), E188 (= E189), E195 (= E196), G240 (= G241), G242 (= G243), H244 (= H245), R297 (= R298), Y302 (= Y303), E303 (= E304), A304 (= A305), R315 (= R316), R334 (= R338)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
49% identity, 97% coverage: 12:444/447 of query aligns to 7:441/444 of P12425

query
sites
P12425

D

E

D

D

V

I

I

E

R

K

A

L

V

V

K

K

E

E

Y

E

N

N

V

V

S

K

F

Y

V

I

Q

R

F

L

W

Q

F

F

V

T

D

D

I

I

L

L

G

G

N

T

L

I

K

K

S

N

F

V

Q

E

V

I

T

P

P

V

S

S

E

Q

L

L

E

G

A

K

A

A

F

L

E

D

E

N

G

K

M

V

G

M

F

F

D

D

G

G

S

S

I

I

L

E

G
|
G

F

F

T

V

R
|
R

I

I

E

E

S

S

D

D

M

M

V

Y

A

L

F

Y

P

P

D

D

A

L

T

N

T

T

F

F

Q

V

I

I

C

F

S

P

W

W

R

T

P

A

L

E

E

K

R

G

P

K

V

V

A

A

R

R

M

F

F

I

C

C

D

D

I

I

R

Y

T

N

P

P

D

D

G

G

T

T

P

P

Y

F

E

E

G

G

D

D

P

P

R

R

Y

N

I

N

L

L

R

K

K

R

L

I

I

L

D

K

K

E

A

M

A

E

Q

D

K

L

G

G

Y

F

T

S

-

D

F

F

Y

N

V

L

G

G

P

P

E
|
E

L

P

E
|
E

F

F

F

L

F

F

S

K

S

L

P

D

Q

E

C

K

P

G

T

E

P

P

I

T

-

L

-

E

-

L

-

N

D

D

A

K

G

G

Y

Y

F

F

D

D

A

L

P

A

P

P

L

T

D

D

L

L

G

G

N

E

D

N

V

C

R

R

D

D

I

I

I

V

F

L

A

E

L

L

Q

E

R

E

M

M

G

G

I

F

P

E

V

I

E

E

Y

A

S

S

H

H

E
|
E

V
|
V

A

A

P

P

S

G

Q

Q

H

H

E
|
E

I

I

D

D

L

F

R

K

Y

Y

N

A

E

G

A

A

M

V

K

R

M

S

A

C

D

D

V

D

V

I

M

Q

T

T

Y

F

K

K

V

L

V

V

K

K

E

T

M

I

A

A

R

R

K

K

H

H

G

G

I

L

Y

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

L

F

F

G

G

E

V

N

N

G
|
G

S

S

G

G

M

M

H
|
H

V

C

H

N

Q

L

S

S

L

L

F

F

R

K

N

N

G

G

K

V

N

N

A

A

F

F

D

D

P

E

N

N

D

A

Q

D

H

L

N

Q

L

L

S

S

H

E

E

T

C

A

R

K

S

H

Y

F

I

I

A

A

G

G

L

I

L

V

K

K

H

H

A

A

R

T

E

S

F

F

C

T

C

A

V

V

T

T

N

N

Q

P

W

T

I

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G
|
G

Y

Y

E
|
E

A

A

P
|
P

V

C

Y

Y

I

V

A

A

W

W

A

S

Q

A

R

Q

N

N

R

R

S

S

A

P

L

L

I

I

R

R

V

I

P

P

M

A

Y

-

K

-

P

-

G

S

K

R

E

G

A

I

A

S

T

T

R

R

I

V

E
|
E

L

V

R

R

S

S

P

V

D

D

P

P

A

A

C

A

N

N

P

P

Y

Y

L

L

A

A

F

L

A

S

V

V

M

L

L

L

G

A

A

A

G

G

L

L

E

D

G

G

I

I

E

K

N

N

N

K

Y

L

E

E

L

A

P

P

K

A

A

P

V

I

E

D

A

R

N

N

I

I

F

Y

H

V

M

M

G

S

E

K

E

E

D

E

L

R

G

M

K

E

H

N

G

G

I

I

G

V

S

D

L

L

P

P

G

A

S

T

L

L

Y

A

E

E

A

A

A

L

M

E

E

E

L

F

R

K

N

S

S

N

N

E

L

V

M

M

K

V

E

K

I

A

L

L

G

G

E

E

H

H

T

L

H

F

A

E

N

H

I

F

V

I

G

E

N

A

K

K

L

E

I

I

E
|
E

W

W

D

D

D

M

Y

F

R

R

T

T

H

Q

I

V

S

H

E

P

F

W

E

E

L

R

K

E

R

Q

Y

Y

L

M

G59 (= G64) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (= R67) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E136) binding
E134 (= E138) binding
E189 (= E189) binding
V190 (= V190) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E196) binding
G241 (= G241) binding
H245 (= H245) binding
G302 (= G302) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (= E304) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P306) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E336) binding
E424 (= E427) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4s0rD Structure of gs-tnra complex (see paper)
49% identity, 97% coverage: 12:444/447 of query aligns to 10:444/447 of 4s0rD

query
sites
4s0rD

D

E

D

D

V

I

I

E

R

K

A

L

V

V

K

K

E

E

Y

E

N

N

V

V

S

K

F

Y

V

I

Q

R

F

L

W

Q

F

F

V

T

D

D

I

I

L

L

G

G

N

T

L

I

K

K

S

N

F

V

Q

E

V

I

T

P

P

V

S

S

E

Q

L

L

E

G

A

K

A

A

F

L

E

D

E

N

G

K

M

V

G

M

F

F

D
|
D

G

G

S

S

I

I

L

E

G

G

F
|
F

T
x
V

R
|
R

I
|
I

E

E

S

S

D

D

M

M

V

Y

A

L

F

Y

P

P

D

D

A

L

T

N

T

T

F

F

Q

V

I

I

C

F

S

P

W

W

R

T

P

A

L

E

E

K

R

G

P

K

V

V

A

A

R

R

M

F

F

I

C

C

D

D

I

I

R

Y

T

N

P

P

D

D

G

G

T

T

P

P

Y

F

E

E

G

G

D

D

P

P

R

R

Y

N

I

N

L

L

R

K

K

R

L

I

I

L

D

K

K

E

A

M

A

E

Q

D

K

L

G

G

Y

F

T

S

-

D

F

F

Y

N

V

L

G

G

P

P

E
|
E

L

P

E
|
E

F

F

F

L

F

F

S

K

S

L

P

D

Q

E

C

K

P

G

T

E

P

P

I

T

-

L

-

E

-

L

-

N

D

D

A

K

G

G

Y

Y

F

F

D
|
D

A

L

P

A

P

P

L

T

D

D

L

L

G

G

N

E

D

N

V

C

R

R

D

D

I

I

I

V

F

L

A

E

L

L

Q

E

R

E

M

M

G

G

I

F

P

E

V

I

E

E

Y

A

S

S

H

H

E
|
E

V
|
V

A
|
A

P
|
P

S

G

Q

Q

H

H

E
|
E

I

I

D

D

L

F

R

K

Y

Y

N

A

E

G

A

A

M

V

K

R

M

S

A

C

D

D

V

D

V

I

M

Q

T

T

Y

F

K

K

V

L

V

V

K

K

E

T

M

I

A

A

R

R

K

K

H

H

G

G

I

L

Y

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

L

F

F

G
|
G

E
x
V

N
|
N

G
|
G

S

S

G
|
G

M

M

H
|
H

V

C

H

N

Q

L

S

S

L

L

F

F

R

K

N

N

G

G

K

V

N

N

A

A

F

F

D

D

P

E

N

N

D

A

Q

D

H

L

N

Q

L

L

S

S

H

E

E

T

C

A

R

K

S

H

Y

F

I

I

A

A

G

G

L

I

L

V

K

K

H

H

A

A

R

T

E

S

F

F

C

T

C

A

V

V

T

T

N

N

Q

P

W

T

I

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G
|
G

Y
|
Y

E
|
E

A
|
A

P

P

V

C

Y

Y

I

V

A

A

W

W

A

S

Q

A

R

Q

N

N

R
|
R

S

S

A

P

L

L

I

I

R

R

V

I

P

P

M

A

Y

-

K

-

P

-

G

S

K

R

E

G

A

I

A

S

T

T

R

R

I

V

E
|
E

L

V

R
|
R

S

S

P

V

D

D

P

P

A

A

C

A

N

N

P

P

Y

Y

L

L

A

A

F

L

A

S

V

V

M

L

L

L

G

A

A

A

G

G

L

L

E

D

G

G

I

I

E

K

N

N

N

K

Y

L

E

E

L

A

P

P

K

A

A

P

V

I

E

D

A

R

N
|
N

I

I

F
x
Y

H

V

M
|
M

G

S

E

K

E

E

D

E

L

R

G

M

K

E

H

N

G

G

I

I

G

V

S

D

L

L

P

P

G

A

S

T

L

L

Y

A

E

E

A

A

A

L

M

E

E

E

L

F

R
x
K

N
x
S

S

N

N

E

L

V

M

M

K

V

E

K

I

A

L

L

G

G

E

E

H

H

T

L

H

F

A

E

N
x
H

I

F

V

I

G

E

N

A

K

K

L

E

I
|
I

E

E

W

W

D

D

D
x
M

Y

F

R

R

T

T

H

Q

I

V

S

H

E

P

F

W

E

E

L

R

K

E

R
x
Q

Y

Y

L

M

active site: D56 (= D58), E135 (= E136), E137 (= E138), E192 (= E189), E199 (= E196), H248 (= H245), R319 (= R316), E336 (= E336), R338 (= R338)
binding glutamine: E137 (= E138), E192 (= E189), E199 (= E196), G244 (= G241), G246 (= G243), R301 (= R298), Y306 (= Y303), E307 (= E304), A308 (= A305)
binding magnesium ion: I66 (= I68), E135 (= E136), E135 (= E136), E199 (= E196), H248 (= H245), H248 (= H245), E336 (= E336), K403 (≠ R403), S404 (≠ N404), H419 (≠ N419)
binding : F63 (= F65), V64 (≠ T66), R65 (= R67), I66 (= I68), D161 (= D158), V193 (= V190), A194 (= A191), P195 (= P192), G241 (= G238), V242 (≠ E239), N243 (= N240), G305 (= G302), Y306 (= Y303), R319 (= R316), N374 (= N374), Y376 (≠ F376), M378 (= M378), I426 (= I426), M430 (≠ D430), Q442 (≠ R442)

Sites not aligning to the query:

binding : 446

P9WN37 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
50% identity, 97% coverage: 14:446/447 of query aligns to 8:446/446 of P9WN37

query
sites
P9WN37

V

V

I

L

R

R

A

T

V

L

K

E

E

E

Y

R

N

D

V

I

S

R

F

F

V

V

Q

R

F

L

W

W

F

F

V

T

D

D

I

V

L

L

G

G

N

F

L

L

K

K

S

S

F

V

Q

A

V

I

T

A

P

P

S

A

E

E

L

L

E

E

A

G

A

A

F

F

E

E

G

G

M

I

G

G

F

F

D

D

G

G

S

S

I

I

L

E

G

G

F

F

T

A

R

R

I

V

E

S

E

E

S

S

D

D

M

T

V

V

A

A

F

H

P

P

D

D

A

P

T

S

T

T

F

F

Q

Q

I

V

C

L

S

P

W

W

R

A

P

T

L

S

E

S

-

G

-

H

R

H

P

H

V

S

A

A

R

R

M

M

F

F

C

C

D

D

I

I

R

T

T

M

P

P

D

D

G

G

T

S

P

P

Y

S

E

W

G

A

D

D

P

P

R

R

Y

H

I

V

L

L

R

R

K

R

L

Q

I

L

D

T

K

K

A

A

A

G

Q

E

K

L

G

G

Y

F

T

S

F

C

Y

Y

V

V

G

H

P

P

E

E

L

I

E

E

F

F

F

L

S

K

-

P

S

G

P

P

Q

E

-

D

-

G

-

S

C

V

P

P

T

V

P

P

I

V

D

D

A

N

G

A

G

G

Y

Y

F

F

D

D

A

Q

P

A

P

V

L

H

D

D

L

S

G

A

N

L

D

N

V

F

R

R

D

H

I

A

I

I

F

D

A

A

L

L

Q

E

R

F

M

M

G

G

I

I

P

S

V

V

E

E

Y

F

S

S

H

H

E

E

V

G

A

A

P

P

S

G

Q

Q

H

Q

E

E

I

I

D

D

L

L

R

R

Y

F

N

A

E

D

A

A

M

L

K

S

M

M

A

A

D

D

V

N

V

V

M

M

T

T

Y

F

K

R

V

Y

V

V

V

I

K

K

E

E

M

V

A

A

R

L

K

E

H

E

G

G

I

A

Y

R

A

A

T

S

F

F

M

M

P

P

K

K

P

P

I

F

F

G

G

Q

E

H

N

P

G

G

S

S

G

A

M

M

H

H

V

T

H

H

Q

M

S

S

L

L

F

F

R

E

N

G

G

D

K

V

N

N

A

A

F

F

F

H

D

S

P

A

N

D

D

D

Q

P

H

L

N

Q

L

L

S

S

H

E

E

V

C

G

R

K

S

S

Y

F

I

I

A

A

G

G

L

I

L

L

K

E

H

H

A

A

R

C

E

E

F

I

C

S

C

A

V

V

T

T

N

N

Q

Q

W

W

I

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

Q

G

G

Y

G

E

E

A

A

P

P

V

T

Y

A

I

A

A

S

W

W

A

G

Q

A

R

A

N

N

R

R

S

S

A

A

L

L

I

V

R

R

V

V

P

P

M

M

Y

Y

K

T

P

P

G

H

K

K

E

T

A

S

T

R

R

R

I

V

E

E

L

V

R

R

S

S

P

P

D

D

P

S

A

A

C

C

N

N

P

P

Y

Y

L

L

A

T

F

F

A

A

V

V

M

L

L

L

G

A

A

A

G

G

L

L

E

R

G

G

I

V

E

E

N
x
K

N

G

Y

Y

E

V

L

L

P

G

K

P

A

Q

V

A

E

E

A

D

N

N

I

V

F

W

H

D

M

L

G

T

E

P

E

E

D

E

L

R

G

R

K

A

H

M

G

G

I

Y

G

R

S

E

L

L

P

P

G

S

S

S

L

L

Y

D

E

S

A

A

A

L

M

R

E

A

L

M

R

E

N

A

S

S

N

E

L

L

M

V

K

A

E

E

I

A

L

L

G

G

E

E

H

H

T

V

H

F

A

D

N

F

I

F

V

L

G

R

N

N

K

K

L

R

I

T

E

E

W

W

D

A

D

N

Y

Y

R

R

T

S

H

H

I

V

S

T

E

P

F

Y

E

E

L

L

K

R

R

T

Y

Y

L

L

P

S

I

L

K363 (≠ N363) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

7tdvC Glutamine synthetase (see paper)
49% identity, 97% coverage: 12:444/447 of query aligns to 6:440/443 of 7tdvC

query
sites
7tdvC

D

E

D

D

V

I

I

R

R

K

A

F

V

V

K

E

E

E

Y

E

N

N

V

V

S

R

F

Y

V

L

Q

R

F

L

W

Q

F

F

V

T

D

D

I

I

L

L

G

G

N

T

L

I

K

K

S

N

F

V

Q

E

V

V

T

P

P

V

S

S

E

Q

L

L

E

E

A

K

A

V

F

L

E

D

E

N

G

E

M

M

G

M

F

F

D

D

G

G

S

S

I

I

L

E

G

G

F

F

T

V

R

R

I

I

E

E

S

S

D

D

M

M

V

Y

A

L

F

H

P

P

D

D

A

L

T

D

T

T

F

W

Q

V

I

I

C

F

S

P

W

W

R

T

P

A

L

G

E

Q

R

G

P

K

V

V

A

A

R

R

M

L

F

I

C

C

D

D

I

V

R

Y

T

K

P

T

D

D

G

G

T

T

P

P

Y

F

E

E

G

G

D

D

P

P

R

R

Y

A

I

N

L

L

R

K

K

R

L

V

I

L

D

K

K

E

A

M

A

E

Q

D

K

L

G

G

Y

F

T

T

-

D

F

F

Y
x
N

V

L

G
|
G

P
|
P

E
|
E

L

P

E
|
E

F

F

F

L

F

F

S

K

S

L

P

D

Q

E

C

K

P

G

T

E

P

P

I

T

-

L

-

E

-

L

-

N

D

D

A

D

G

G

Y

Y

F

F

D

D

A

L

P

A

P

P

L

T

D

D

L

L

G

G

N

E

D

N

V

C

R

R

D

D

I

I

I

V

F

L

A

E

L

L

Q

E

R

D

M

M

G

G

I

F

P

D

V

I

E
|
E

Y

A

S

S

H

H

E
|
E

V

V

A

A

P

P

S

G

Q
|
Q

H

H

E
|
E

I

I

D
|
D

L
x
F

R
x
K

Y
|
Y

N

A

E

D

A

A

M

V

K

T

M

A

A

C

D

D

V

N

V

I

M

Q

T

T

Y

F

K

K

V

L

V

V

K

K

E

T

M

I

A

A

R

R

K

K

H

H

G

N

I

L

Y

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

L

F

F

G

G

E

V

N

N

G
|
G

S

S

G
|
G

M

M

H
|
H

V

F

H
x
N

Q
x
V

S
|
S

L

L

F

F

R

K

N

G

G

K

K

E

N

N

A

A

F

F

D
|
D

P

P

N

N

D

T

Q

E

H

M

N

G

L

L

S

T

H
x
E

E

T

C

A

R

Y

S

Q

Y

F

I

T

A

A

G

G

L

V

L

L

K

K

H

N

A

A

R

R

E

G

F

F

C

T

C

A

V

V

T

C

N

N

Q

P

W

L

I

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G

G

Y
|
Y

E
|
E

A
|
A

P

P

V

C

Y

Y

I

I

A

A

W

W

A

S

Q

G

R

K

N

N

R
|
R

S

S

A

P

L

L

I

I

R
|
R

V

V

P

P

M

-

Y

-

K

-

P

S

G

S

K

R

E

G

A
x
L

A
x
S

T
|
T

R
|
R

I

I

E
|
E

L

V

R
|
R

S

S

P

V

D

D

P

P

A

A

C

A

N

N

P

P

Y

Y

L

M

A

A

F

L

A

A

V

A

M

I

L

L

G

E

A

A

G

G

L

L

E

D

G

G

I

I

E

K

N

N

N

K

Y

L

E

K

L

V

P

P

K

E

A

P

V

V

E

N

A

Q

N

N

I

I

F

Y

H

E

M

M

G

N

E

R

E

E

D

E

L

R

G

E

K

A

H

V

G

G

I

I

G

Q

S

D

L

L

P

P

G

S

S

T

L

L

Y

Y

E

T

A

A

A

L

M

K

E

A

L

M

R

R

N

E

S

N

N

E

L

V

M

I

K

K

E

K

I

A

L

L

G

G

E

N

H

H

T

I

H

Y

A

N

N

Q

I

F

V

I

G

N

N

S

K

K

L

S

I

I

E

E

W

W

D

D

D

Y

Y

Y

R

R

T

T

H

Q

I

V

S

S

E

E

F

W

E

E

L

R

K

D

R

Q

Y

Y

L

M

binding adenosine-5'-diphosphate: N127 (≠ Y132), G129 (= G134), P130 (= P135), E131 (= E136), E183 (= E184), E195 (= E196), D197 (= D198), F198 (≠ L199), K199 (≠ R200), Y200 (= Y201), N246 (≠ H247), V247 (≠ Q248), S248 (= S249), R315 (= R316), R320 (= R321), L327 (≠ A331), S328 (≠ A332), T329 (= T333), R330 (= R334), E332 (= E336)
binding magnesium ion: E131 (= E136), E131 (= E136), E133 (= E138), E188 (= E189), E195 (= E196), E195 (= E196), D197 (= D198), H244 (= H245), D259 (= D260), E268 (≠ H269), E332 (= E336)
binding l-methionine-s-sulfoximine phosphate: E131 (= E136), E133 (= E138), E188 (= E189), Q193 (= Q194), E195 (= E196), G240 (= G241), G242 (= G243), H244 (= H245), R297 (= R298), Y302 (= Y303), E303 (= E304), A304 (= A305), R315 (= R316), R334 (= R338)

7tf6A Glutamine synthetase (see paper)
49% identity, 97% coverage: 12:444/447 of query aligns to 5:435/438 of 7tf6A

query
sites
7tf6A

D

E

D

D

V

I

I

R

R

K

A

F

V

A

K

E

E

E

Y

E

N

N

V

V

S

R

F

Y

V

L

Q

R

F

L

W

Q

F

F

V

T

D

D

I

I

L

L

G

G

N

T

L

I

K

K

S

N

F

V

Q

E

V

V

T

P

P

V

S

S

E

Q

L

L

E

E

A

K

A

V

F

L

E

D

E

N

G

E

M

M

G

M

F

F

D

D

G

G

S

S

I

I

L

E

G

G

F
|
F

T
x
V

R
|
R

I

I

E

E

S

S

D

D

M

M

V

Y

A

L

F

H

P

P

D

D

A

L

T

D

T

T

F

W

Q

V

I

I

C

F

S

P

W

W

R

G

P

K

L

-

E

-

R

-

P

-

V

V

A

A

R

R

M

L

F

I

C

C

D

D

I

V

R

Y

T

K

P

T

D

D

G

G

T

T

P

P

Y

F

E

E

G

G

D

D

P

P

R

R

Y

A

I

N

L

L

R

K

K

R

L

V

I

L

D

K

K

E

A

M

A

E

Q

D

K

L

G

G

Y

F

T

T

-

D

F

F

Y

N

V

L

G

G

P

P

E
|
E

L

P

E
|
E

F

F

F

L

F

F

S

K

S

L

P

D

Q

E

C

K

P

G

T

E

P

P

I

T

-

L

-

E

-

L

-

N

D

D

A

D

G

G

Y
|
Y

F

F

D

D

A

L

P

A

P

P

L

T

D

D

L

L

G

G

N

E

D

N

V

C

R

R

D

D

I

I

I

V

F

L

A

E

L

L

Q

E

R

D

M

M

G

G

I

F

P

D

V

I

E

E

Y

A

S

S

H

H

E
|
E

V
|
V

A

A

P

P

S

G

Q

Q

H

H

E
|
E

I

I

D

D

L

F

R

K

Y

Y

N

A

E

D

A

A

M

V

K

T

M

A

A

C

D

D

V

N

V

I

M

Q

T

T

Y

F

K

K

V

L

V

V

K

K

E

T

M

I

A

A

R

R

K

K

H

H

G

N

I

L

Y

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

L

F

F

G
|
G

E

V

N
|
N

G
|
G

S
|
S

G
|
G

M

M

H
|
H

V

F

H

N

Q

V

S

S

L

L

F

F

R

K

N

G

G

K

K

E

N

N

A

A

F

F

D

D

P

P

N

N

D

T

Q

E

H

M

N

G