SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 209006 MicrobesOnline__882:209006 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

5uhsA Structure of a semisweet d57a mutant (see paper)
39% identity, 68% coverage: 6:82/113 of query aligns to 3:79/81 of 5uhsA

query
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5uhsA

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binding beta-D-glucopyranose: F41 (≠ L44), W48 (= W51)

B0SR19 Sugar transporter SemiSWEET from Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris) (see paper)
36% identity, 73% coverage: 6:88/113 of query aligns to 3:85/85 of B0SR19

query
sites
B0SR19

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W48 (= W51) mutation to A: Impairs glucose transport.
N64 (= N67) mutation to A: Impairs glucose transport.

P0DMV3 Sugar transporter SemiSWEET from Escherichia coli (strain UMEA 3162-1) (see paper)
33% identity, 71% coverage: 3:82/113 of query aligns to 2:81/89 of P0DMV3

query
sites
P0DMV3

D

D

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F19 (≠ Y20) mutation to A: Decreases sucrose transporter activity.
P21 (= P22) mutation to A: Strongly decreases sucrose transporter activity.
Q22 (= Q23) mutation to A: Slightly decreases sucrose transporter activity.
M39 (≠ T40) mutation to A: No effect on sucrose transporter activity.
Y40 (≠ F41) mutation to A: Decreases sucrose transporter activity.
W50 (= W51) mutation to A: Increases sucrose transporter activity.; mutation to F: No effect on sucrose transporter activity.
Y53 (= Y54) mutation to F: Increases sucrose transporter activity.
R57 (= R58) mutation to A: Increases sucrose transporter activity.
N66 (= N67) mutation to A: Impairs sucrose transporter activity.

Query Sequence

>209006 MicrobesOnline__882:209006
MPDTLDLIGYAAGLLTSLAYVPQVVRLWRTRSVADISLPTFCLLTVGIGLWLLYGMGREA
GPVIVANAVGMLLTLAIVVMKLWFGRHSAQATDEGIGHPVRQDAAKPHLRRDA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory