SitesBLAST

C26 (≠ N16) mutation to A: Lower ATPase activity and transport efficiency.
F27 (= F17) mutation to L: Lower ATPase activity and transport efficiency.
F45 (= F35) mutation to L: Lower ATPase activity and transport efficiency.
C54 (= C44) mutation to T: Loss of ATPase activity and transport.
L60 (= L50) mutation to F: Lower ATPase activity and transport efficiency.
L76 (= L66) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V125) mutation to M: Loss of ATPase activity and transport.
D172 (= D162) mutation to N: Loss of ATPase activity and transport.
C276 (≠ F266) mutation to A: Lower ATPase activity and transport efficiency.
E297 (= E287) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
44% identity, 86% coverage: 8:324/368 of query aligns to 7:323/375 of 2d62A

query
sites
2d62A

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binding pyrophosphate 2-: G42 (= G43), C43 (= C44), G44 (= G45), K45 (= K46), T46 (= T47), T47 (= T48)

1g291 Malk (see paper)
41% identity, 91% coverage: 8:341/368 of query aligns to 4:345/372 of 1g291

query
sites
1g291

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V

binding magnesium ion: D69 (= D73), E71 (vs. gap), K72 (vs. gap), K79 (≠ E77), D80 (≠ A78), E292 (= E287), D293 (= D288)
binding pyrophosphate 2-: S38 (= S42), G39 (= G43), C40 (= C44), G41 (= G45), K42 (= K46), T43 (= T47), T44 (= T48)

Sites not aligning to the query:

binding magnesium ion: 359

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
50% identity, 63% coverage: 8:240/368 of query aligns to 4:237/393 of P9WQI3

query
sites
P9WQI3

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H

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D

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A

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S

H193 (= H196) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

8hprC Lpqy-sugabc in state 4 (see paper)
49% identity, 63% coverage: 8:240/368 of query aligns to 3:236/363 of 8hprC

query
sites
8hprC

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D

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A

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binding adenosine-5'-triphosphate: Y12 (≠ F17), S38 (= S42), G39 (= G43), G41 (= G45), K42 (= K46), S43 (≠ T47), Q82 (= Q86), Q133 (= Q137), G136 (= G140), G137 (= G141), Q138 (= Q142), H192 (= H196)
binding magnesium ion: S43 (≠ T47), Q82 (= Q86)

8hprD Lpqy-sugabc in state 4 (see paper)
49% identity, 63% coverage: 8:240/368 of query aligns to 3:236/362 of 8hprD

query
sites
8hprD

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D

R

R

Q

D

V

I

N

A

T

M

V

V

F

F

Q
|
Q

N

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

S

T

V

V

R

R

E

Q

N

N

V

I

G

A

F

F

G

P

L

L

R

T

M

L

Q

A

R

K

R

V

P

P

K

K

D

A

E

E

I

I

A

A

R

A

R

K

V

V

H

E

D

E

A

T

L

A

R

K

M

I

V

L

H

D

L

L

E

S

A

E

H

L

A

L

D

D

R
|
R

R

K

P

P

R

G

Q
|
Q

L

L

S
|
S

G
|
G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

M

A

G

R

R

A

A

V

I

V

V

N

R

N

S

P

P

L

K

V

A

L

F

L

L

L

M

D

D

E
x
Q

P

P

F

L

S

S

A

N

L

L

D

D

Y

A

K

K

L

L

R

R

K

V

Q

Q

M

M

Q

R

L

A

E

E

I

I

K

S

H

R

L

L

Q

Q

R

D

Q

R

L

L

G

G

I

T

T

T

F

T

V

V

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

T

E

E

A

A

F

M

A

T

M

L

S

G

D

D

R

R

V

V

M

M

N

L

D

A

G

G

K

E

I

V

E

Q

Q

Q

I

I

G

G

S

T

P

P

Q

D

E

E

I

L

Y

Y

E

S

P

P

A

A

N

N

L

L

Y

F

V

V

A

A

R

G

F

F

V

I

G

G

E

S

binding adenosine-5'-triphosphate: Y12 (≠ F17), S38 (= S42), C40 (= C44), G41 (= G45), K42 (= K46), S43 (≠ T47), T44 (= T48), Q82 (= Q86), R129 (= R133), Q133 (= Q137), S135 (= S139), G136 (= G140), G137 (= G141), Q159 (≠ E163), H192 (= H196)
binding magnesium ion: S43 (≠ T47), Q82 (= Q86)

8hplC Lpqy-sugabc in state 1 (see paper)
48% identity, 63% coverage: 8:240/368 of query aligns to 3:234/384 of 8hplC

query
sites
8hplC

I

I

E

V

L

L

R

D

G

R

V

V

T

T

K

K

N

S

F
x
Y

E

P

D

-

T

R

C

A

A

A

L

V

D

K

N

E

I

F

D

S

L

M

E

T

I

I

R

A

N

D

G

G

E

E

F

F

L

I

T

I

L

L

L

V

G

G

P

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

I

T

L

L

R

N

L

M

I

I

S

A

G

G

F

L

E

E

K

E

P

I

D

T

A

S

G

G

V

E

I

L

T

R

L

I

K

G

G

G

Q

E

R

R

M

V

D

N

D

E

A

K

P

A

P

P

E

K

A

D

R

R

Q

D

V

I

N

A

T

M

V

V

F

F

Q

Q

N

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

S

T

V

V

R

R

E

Q

N

N

V

I

G

A

F

F

G

P

L

L

R

T

M

L

Q

A

R

K

R

V

P

P

K

K

D

A

E

E

I

I

A

A

R

A

R

K

V

V

H

E

D

E

A

T

L

A

R

K

M

I

V

L

H

D

L

L

E

S

A

E

H

L

A

L

D

D

R

R

R

K

P

P

R

G

Q

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

M

A

G

R

R

A

A

V

I

V

V

N

R

N

S

P

P

L

K

V

A

L

F

L

L

L

M

D

D

E

Q

P

P

F

L

S

S

A

N

L

L

D

D

Y

A

K

K

L

L

R

R

K

V

Q

Q

M

M

Q

R

L

A

E

E

I

I

K

S

H

R

L

L

Q

Q

R

D

Q

R

L

L

G

G

I

T

T

T

F

T

V

V

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

T

E

E

A

A

F

M

A

T

M

L

S

G

D

D

R

R

V

V

M

M

N

L

D

A

G

G

K

E

I

V

E

Q

Q

Q

I

I

G

G

S

T

P

P

Q

D

E

E

I

L

Y

Y

E

S

P

P

A

A

N

N

L

L

Y

F

V

V

A

A

R

G

F

F

V

I

G

G

E

S

binding adenosine-5'-triphosphate: Y12 (≠ F17), G37 (= G43), C38 (= C44), G39 (= G45), K40 (= K46), S41 (≠ T47), T42 (= T48)

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
42% identity, 77% coverage: 8:289/368 of query aligns to 3:289/371 of 3puyA

query
sites
3puyA

I

V

E

Q

L

L

R

Q

G

N

V

V

T

T

K

K

N

A

F
x
W

E

G

D

E

T

V

C

V

A

V

L

S

D

K

N

D

I

I

D

N

L

L

E

D

I

I

R

H

N

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

I

L

L

L

R

R

L

M

I

I

S

A

G

G

F

L

E

E

K

T

P

I

D

T

A

S

G

G

V

D

I

L

T

F

L

I

K

G

G

E

Q

K

R

R

M

M

D

N

D

D

A

T

P

P

E

A

A

E

R

R

Q

G

V

V

N

G

T

M

V

V

F

F

Q
|
Q

N

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

R

A

E

E

N

N

V

M

G

S

F

F

G

G

L

L

R

K

M

L

Q

A

R

G

R

A

P

K

K

K

D

E

E

V

I

I

A

N

R

Q

R

R

V

V

H

N

D

Q

A

V

L

A

R

E

M

V

V

L

H

Q

L

L

E

A

A

H

H

L

A

L

D

D

R
|
R

R

K

P

P

R

K

Q
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

N

A

N

E

P

P

L

S

V

V

L

F

L

L

D

D

E
|
E

P

P

F

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

K

V

Q

Q

M

M

Q

R

L

I

E

E

I

I

K

S

H

R

L

L

Q

H

R

K

Q

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

F

M

A

T

M

L

S

A

D

D

R

K

V

I

V

V

M

L

N

D

D

A

G

G

K

R

I

V

E

A

Q

Q

I

V

G

G

S

K

P

P

Q

L

E

E

I

L

Y

Y

E

H

E

Y

P

P

A

A

N

D

L

R

Y

F

V

V

A

A

R

G

F

F

V

I

G

G

-

S

-

P

E

K

I

M

N

N

I

F

L

L

N

P

A

V

V

K

I

V

A

T

A

A

N

T

H

A

G

I

D

D

G

Q

L

V

Y

Q

D

V

A

E

V

L

I

P

-

M

-

P

-

N

-

R

E

Q

G

Q

V

V

T

W

F

L

P

P

I

V

R

E

S

S

Q

-

R

R

T

D

F

V

A

Q

P

V

G

G

D

A

K

N

V

M

N

S

V

L

L

G

L

I

R

R

P

P

E

E

D

H

L

L

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F17), S37 (= S42), G38 (= G43), C39 (= C44), G40 (= G45), K41 (= K46), S42 (≠ T47), T43 (= T48), Q81 (= Q86), R128 (= R133), A132 (≠ Q137), S134 (= S139), G136 (= G141), Q137 (= Q142), E158 (= E163), H191 (= H196)
binding magnesium ion: S42 (≠ T47), Q81 (= Q86)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
42% identity, 77% coverage: 8:289/368 of query aligns to 3:289/371 of 3puxA

query
sites
3puxA

I

V

E

Q

L

L

R

Q

G

N

V

V

T

T

K

K

N

A

F
x
W

E

G

D

E

T

V

C

V

A

V

L

S

D

K

N

D

I

I

D

N

L

L

E

D

I

I

R

H

N

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

I

L

L

L

R

R

L

M

I

I

S

A

G

G

F

L

E

E

K

T

P

I

D

T

A

S

G

G

V

D

I

L

T

F

L

I

K

G

G

E

Q

K

R

R

M

M

D

N

D

D

A

T

P

P

E

A

A

E

R

R

Q

G

V

V

N

G

T

M

V

V

F

F

Q
|
Q

N

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

R

A

E

E

N

N

V

M

G

S

F

F

G

G

L

L

R

K

M

L

Q

A

R

G

R

A

P

K

K

K

D

E

E

V

I

I

A

N

R

Q

R

R

V

V

H

N

D

Q

A

V

L

A

R

E

M

V

V

L

H

Q

L

L

E

A

A

H

H

L

A

L

D

D

R
|
R

R

K

P

P

R

K

Q

A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

N

A

N

E

P

P

L

S

V

V

L

F

L

L

D

D

E

E

P

P

F

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

K

V

Q

Q

M

M

Q

R

L

I

E

E

I

I

K

S

H

R

L

L

Q

H

R

K

Q

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

F

M

A

T

M

L

S

A

D

D

R

K

V

I

V

V

M

L

N

D

D

A

G

G

K

R

I

V

E

A

Q

Q

I

V

G

G

S

K

P

P

Q

L

E

E

I

L

Y

Y

E

H

E

Y

P

P

A

A

N

D

L

R

Y

F

V

V

A

A

R

G

F

F

V

I

G

G

-

S

-

P

E

K

I

M

N

N

I

F

L

L

N

P

A

V

V

K

I

V

A

T

A

A

N

T

H

A

G

I

D

D

G

Q

L

V

Y

Q

D

V

A

E

V

L

I

P

-

M

-

P

-

N

-

R

E

Q

G

Q

V

V

T

W

F

L

P

P

I

V

R

E

S

S

Q

-

R

R

T

D

F

V

A

Q

P

V

G

G

D

A

K

N

V

M

N

S

V

L

L

G

L

I

R

R

P

P

E

E

D

H

L

L

binding adenosine-5'-diphosphate: W12 (≠ F17), G38 (= G43), C39 (= C44), G40 (= G45), K41 (= K46), S42 (≠ T47), T43 (= T48), R128 (= R133), S134 (= S139), Q137 (= Q142)
binding beryllium trifluoride ion: S37 (= S42), G38 (= G43), K41 (= K46), Q81 (= Q86), S134 (= S139), G136 (= G141), H191 (= H196)
binding magnesium ion: S42 (≠ T47), Q81 (= Q86)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
42% identity, 77% coverage: 8:289/368 of query aligns to 3:289/371 of 3puwA

query
sites
3puwA

I

V

E

Q

L

L

R

Q

G

N

V

V

T

T

K

K

N

A

F
x
W

E

G

D

E

T

V

C

V

A
x
V

L

S

D

K

N

D

I

I

D

N

L

L

E

D

I

I

R

H

N

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

I

L

L

L

R

R

L

M

I

I

S

A

G

G

F

L

E

E

K

T

P

I

D

T

A

S

G

G

V

D

I

L

T

F

L

I

K

G

G

E

Q

K

R

R

M

M

D

N

D

D

A

T

P

P

E

A

A

E

R

R

Q

G

V

V

N

G

T

M

V

V

F

F

Q
|
Q

N

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

R

A

E

E

N

N

V

M

G

S

F

F

G

G

L

L

R

K

M

L

Q

A

R

G

R

A

P

K

K

K

D

E

E

V

I

I

A

N

R

Q

R

R

V

V

H

N

D

Q

A

V

L

A

R

E

M

V

V

L

H

Q

L

L

E

A

A

H

H

L

A

L

D

D

R
|
R

R

K

P

P

R

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

N

A

N

E

P

P

L

S

V

V

L

F

L

L

D

D

E
|
E

P

P

F

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

K

V

Q

Q

M

M

Q

R

L

I

E

E

I

I

K

S

H

R

L

L

Q

H

R

K

Q

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

F

M

A

T

M

L

S

A

D

D

R

K

V

I

V

V

M

L

N

D

D

A

G

G

K

R

I

V

E

A

Q

Q

I

V

G

G

S

K

P

P

Q

L

E

E

I

L

Y

Y

E

H

E

Y

P

P

A

A

N

D

L

R

Y

F

V

V

A

A

R

G

F

F

V

I

G

G

-

S

-

P

E

K

I

M

N

N

I

F

L

L

N

P

A

V

V

K

I

V

A

T

A

A

N

T

H

A

G

I

D

D

G

Q

L

V

Y

Q

D

V

A

E

V

L

I

P

-

M

-

P

-

N

-

R

E

Q

G

Q

V

V

T

W

F

L

P

P

I

V

R

E

S

S

Q

-

R

R

T

D

F

V

A

Q

P

V

G

G

D

A

K

N

V

M

N

S

V

L

L

G

L

I

R

R

P

P

E

E

D

H

L

L

binding adenosine-5'-diphosphate: W12 (≠ F17), V17 (≠ A22), G38 (= G43), C39 (= C44), G40 (= G45), K41 (= K46), S42 (≠ T47), T43 (= T48), R128 (= R133), A132 (≠ Q137), S134 (= S139), Q137 (= Q142)
binding tetrafluoroaluminate ion: S37 (= S42), G38 (= G43), K41 (= K46), Q81 (= Q86), S134 (= S139), G135 (= G140), G136 (= G141), E158 (= E163), H191 (= H196)
binding magnesium ion: S42 (≠ T47), Q81 (= Q86)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
42% identity, 77% coverage: 8:289/368 of query aligns to 3:289/371 of 3puvA

query
sites
3puvA

I

V

E

Q

L

L

R

Q

G

N

V

V

T

T

K

K

N

A

F
x
W

E

G

D

E

T

V

C

V

A
x
V

L

S

D

K

N

D

I

I

D

N

L

L

E

D

I

I

R

H

N

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

I

L

L

L

R

R

L

M

I

I

S

A

G

G

F

L

E

E

K

T

P

I

D

T

A

S

G

G

V

D

I

L

T

F

L

I

K

G

G

E

Q

K

R

R

M

M

D

N

D

D

A

T

P

P

E

A

A

E

R

R

Q

G

V

V

N

G

T

M

V

V

F

F

Q
|
Q

N

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

R

A

E

E

N

N

V

M

G

S

F

F

G

G

L

L

R

K

M

L

Q

A

R

G

R

A

P

K

K

K

D

E

E

V

I

I

A

N

R

Q

R

R

V

V

H

N

D

Q

A

V

L

A

R

E

M

V

V

L

H

Q

L

L

E

A

A

H

H

L

A

L

D

D

R
|
R

R

K

P

P

R

K

Q
x
A

L

L

S
|
S

G

G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

N

A

N

E

P

P

L

S

V

V

L

F

L

L

D

D

E

E

P

P

F

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

K

V

Q

Q

M

M

Q

R

L

I

E

E

I

I

K

S

H

R

L

L

Q

H

R

K

Q

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

F

M

A

T

M

L

S

A

D

D

R

K

V

I

V

V

M

L

N

D

D

A

G

G

K

R

I

V

E

A

Q

Q

I

V

G

G

S

K

P

P

Q

L

E

E

I

L

Y

Y

E

H

E

Y

P

P

A

A

N

D

L

R

Y

F

V

V

A

A

R

G

F

F

V

I

G

G

-

S

-

P

E

K

I

M

N

N

I

F

L

L

N

P

A

V

V

K

I

V

A

T

A

A

N

T

H

A

G

I

D

D

G

Q

L

V

Y

Q

D

V

A

E

V

L

I

P

-

M

-

P

-

N

-

R

E

Q

G

Q

V

V

T

W

F

L

P

P

I

V

R

E

S

S

Q

-

R

R

T

D

F

V

A

Q

P

V

G

G

D

A

K

N

V

M

N

S

V

L

L

G

L

I

R

R

P

P

E

E

D

H

L

L

binding adenosine-5'-diphosphate: W12 (≠ F17), V17 (≠ A22), G38 (= G43), C39 (= C44), G40 (= G45), K41 (= K46), S42 (≠ T47), T43 (= T48), R128 (= R133), A132 (≠ Q137), S134 (= S139), Q137 (= Q142)
binding magnesium ion: S42 (≠ T47), Q81 (= Q86)

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
42% identity, 77% coverage: 8:289/368 of query aligns to 3:289/374 of 2awnB

query
sites
2awnB

I

V

E

Q

L

L

R

Q

G

N

V

V

T

T

K

K

N

A

F
x
W

E

G

D

E

T

V

C

V

A
x
V

L

S

D

K

N

D

I

I

D

N

L

L

E

D

I

I

R

H

N

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C

C

G
|
G

K
|
K

T
x
S

T
|
T

I

L

L

L

R

R

L

M

I

I

S

A

G

G

F

L

E

E

K

T

P

I

D

T

A

S

G

G

V

D

I

L

T

F

L

I

K

G

G

E

Q

K

R

R

M

M

D

N

D

D

A

T

P

P

E

A

A

E

R

R

Q

G

V

V

N

G

T

M

V

V

F

F

Q

Q

N

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

R

A

E

E

N

N

V

M

G

S

F

F

G

G

L

L

R

K

M

L

Q

A

R

G

R

A

P

K

K

K

D

E

E

V

I

I

A

N

R

Q

R

R

V

V

H

N

D

Q

A

V

L

A

R

E

M

V

V

L

H

Q

L

L

E

A

A

H

H

L

A

L

D

D

R

R

R

K

P

P

R

K

Q

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

N

A

N

E

P

P

L

S

V

V

L

F

L

L

D

D

E

E

P

P

F

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

K

V

Q

Q

M

M

Q

R

L

I

E

E

I

I

K

S

H

R

L

L

Q

H

R

K

Q

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

F

M

A

T

M

L

S

A

D

D

R

K

V

I

V

V

M

L

N

D

D

A

G

G

K

R

I

V

E

A

Q

Q

I

V

G

G

S

K

P

P

Q

L

E

E

I

L

Y

Y

E

H

E

Y

P

P

A

A

N

D

L

R

Y

F

V

V

A

A

R

G

F

F

V

I

G

G

-

S

-

P

E

K

I

M

N

N

I

F

L

L

N

P

A

V

V

K

I

V

A

T

A

A

N

T

H

A

G

I

D

D

G

Q

L

V

Y

Q

D

V

A

E

V

L

I

P

-

M

-

P

-

N

-

R

E

Q

G

Q

V

V

T

W

F

L

P

P

I

V

R

E

S

S

Q

-

R

R

T

D

F

V

A

Q

P

V

G

G

D

A

K

N

V

M

N

S

V

L

L

G

L

I

R

R

P

P

E

E

D

H

L

L

binding adenosine-5'-diphosphate: W12 (≠ F17), V17 (≠ A22), S37 (= S42), G38 (= G43), G40 (= G45), K41 (= K46), S42 (≠ T47), T43 (= T48)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
42% identity, 77% coverage: 8:289/368 of query aligns to 4:290/371 of P68187

query
sites
P68187

I

V

E

Q

L

L

R

Q

G

N

V

V

T

T

K

K

N

A

F

W

E

G

D

E

T

V

C

V

A

V

L

S

D

K

N

D

I

I

D

N

L

L

E

D

I

I

R

H

N

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

I

L

L

L

R

R

L

M

I

I

S

A

G

G

F

L

E

E

K

T

P

I

D

T

A

S

G

G

V

D

I

L

T

F

L

I

K

G

G

E

Q

K

R

R

M

M

D

N

D

D

A

T

P

P

E

A

A

E

R

R

Q

G

V

V

N

G

T

M

V

V

F

F

Q

Q

N

S

Y

Y

A
|
A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

R

A

E

E

N

N

V

M

G

S

F

F

G

G

L

L

R

K

M

L

Q

A

R

G

R

A

P
x
K

K

K

D

E

E

V

I

I

A

N

R

Q

R

R

V
|
V

H

N

D

Q

A
x
V

L

A

R
x
E

M

V

V

L

H

Q

L

L

E
x
A

A

H

H

L

A

L

D

D

R

R

R

K

P

P

R

K

Q

A

L

L

S

S

G

G

G
|
G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

N

A

N

E

P

P

L

S

V

V

L

F

L

L

D
|
D

E

E

P

P

F

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

K

V

Q

Q

M

M

Q

R

L

I

E

E

I

I

K

S

H

R

L

L

Q

H

R

K

Q

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

F

M

A

T

M

L

S

A

D

D

R

K

V

I

V

V

M

L

N

D

D

A

G

G

K

R

I

V

E

A

Q

Q

I

V

G

G

S

K

P

P

Q

L

E

E

I

L

Y

Y

E

H

E

Y

P

P

A

A

N

D

L
x
R

Y

F

V

V

A

A

R

G

F

F

V

I

G

G

-

S

-

P

E

K

I

M

N

N

I
x
F

L

L

N

P

A

V

V

K

I

V

A

T

A

A

N

T

H

A

G

I

D

D

G

Q

L

V

Y

Q

D

V

A

E

V

L

I

P

-

M

-

P

-

N

-

R

E

Q

G

Q

V

V

T
x
W

F

L

P

P

I

V

R

E

S

S

Q

-

R

R

T

D

F

V

A

Q

P

V

G
|
G

D

A

K

N

V

M

N
x
S

V

L

L
x
G

L

I

R

R

P

P

E

E

D

H

L

L

A85 (= A89) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ P110) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V118) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ A121) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ R123) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ E128) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G141) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D162) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ L232) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
F241 (≠ I243) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
W267 (≠ T265) mutation to G: Normal maltose transport but constitutive mal gene expression.
G278 (= G277) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
S282 (≠ N281) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G284 (≠ L283) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
42% identity, 77% coverage: 8:289/368 of query aligns to 1:287/367 of 1q12A

query
sites
1q12A

I

V

E

Q

L

L

R

Q

G

N

V

V

T

T

K

K

N

A

F
x
W

E

G

D

E

T

V

C

V

A

V

L

S

D

K

N

D

I

I

D

N

L

L

E

D

I

I

R

H

N

E

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

I

L

L

L

R

R

L

M

I

I

S

A

G

G

F

L

E

E

K

T

P

I

D

T

A

S

G

G

V

D

I

L

T

F

L

I

K

G

G

E

Q

K

R

R

M

M

D

N

D

D

A

T

P

P

E

A

A

E

R

R

Q

G

V

V

N

G

T

M

V

V

F

F

Q

Q

N

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

R

A

E

E

N

N

V

M

G

S

F

F

G

G

L

L

R

K

M

L

Q

A

R

G

R

A

P

K

K

K

D

E

E

V

I

I

A

N

R

Q

R

R

V

V

H

N

D

Q

A

V

L

A

R

E

M

V

V

L

H

Q

L

L

E

A

A

H

H

L

A

L

D

D

R
|
R

R

K

P

P

R

K

Q
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

G

R

R

A

T

V

L

V

V

N

A

N

E

P

P

L

S

V

V

L

F

L

L

D

D

E

E

P

P

F

L

S

S

A

N

L

L

D

D

Y

A

K

A

L

L

R

R

K

V

Q

Q

M

M

Q

R

L

I

E

E

I

I

K

S

H

R

L

L

Q

H

R

K

Q

R

L

L

G

G

I

R

T

T

F

M

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

F

M

A

T

M

L

S

A

D

D

R

K

V

I

V

V

M

L

N

D

D

A

G

G

K

R

I

V

E

A

Q

Q

I

V

G

G

S

K

P

P

Q

L

E

E

I

L

Y

Y

E

H

E

Y

P

P

A

A

N

D

L

R

Y

F

V

V

A

A

R

G

F

F

V

I

G

G

-

S

-

P

E

K

I

M

N

N

I

F

L

L

N

P

A

V

V

K

I

V

A

T

A

A

N

T

H

A

G

I

D

D

G

Q

L

V

Y

Q

D

V

A

E

V

L

I

P

-

M

-

P

-

N

-

R

E

Q

G

Q

V

V

T

W

F

L

P

P

I

V

R

E

S

S

Q

-

R

R

T

D

F

V

A

Q

P

V

G

G

D

A

K

N

V

M

N

S

V

L

L

G

L

I

R

R

P

P

E

E

D

H

L

L

binding adenosine-5'-triphosphate: W10 (≠ F17), S35 (= S42), G36 (= G43), C37 (= C44), G38 (= G45), K39 (= K46), S40 (≠ T47), T41 (= T48), R126 (= R133), A130 (≠ Q137), S132 (= S139), G134 (= G141), Q135 (= Q142)

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
44% identity, 82% coverage: 8:309/368 of query aligns to 7:296/353 of 1vciA

query
sites
1vciA

I

V

E

K

L

L

R

E

G

N

V

L

T

T

K

K

N

R

F
|
F

E

G

D

N

T
x
F

C

T

A

A

L

V

D

N

N

K

I

L

D

N

L

L

E

T

I

I

R

K

N

D

G

G

E

E

F

F

L

L

T

V

L

L

G

G

P

P

S
|
S

G
|
G

C

C

G
|
G

K
|
K

T
|
T

I

T

L

L

R

R

L

M

I

I

S

A

G

G

F

L

E

E

K

E

P

P

D

T

A

E

G

G

V

R

I

I

T

Y

L

F

K

G

G

D

Q

R

R

D

M

V

D

T

D

Y

A

L

P

P

E

K

A

D

R

R

Q

N

V

I

N

S

T

M

V

V

F

F

Q

Q

N

-

Y

-

A

-

L

-

F

-

P

-

H

H

M

M

S

T

V

V

R

Y

E

E

N

N

V

I

G

A

F

F

G

P

L

L

R

K

M

-

Q

-

R

K

R

F

P

P

K

K

D

D

E

E

I

I

A

D

R

K

R

R

V

V

H

R

D

W

A

A

L

A

R

E

M

L

V

L

H

Q

L

I

E

E

A

E

H

L

A

L

D

N

R

R

R

Y

P

P

R

A

Q

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

A

A

I

V

A

A

R

R

A

A

V

I

V

V

N

V

N

E

P

P

L

D

V

V

L

L

L

M

D

D

E

E

P

P

F

L

S

S

A

N

L

L

D

D

Y

A

K

K

L

L

R

R

K

V

Q

A

M

M

Q

R

L

A

E

E

I

I

K

K

H

K

L

L

Q

Q

R

Q

Q

K

L

L

G

K

I

V

T

T

F

T

V

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

F

M

A

T

M

M

S

G

D

D

R

R

V

I

V

A

V

V

M

M

N

N

D

R

G

G

K

Q

I

L

E

L

Q

Q

I

I

G

G

S

S

P

P

Q

T

E

E

I

V

Y

Y

E

L

E

R

P

P

A

N

N

S

L

V

Y

F

V

V

A

A

R

T

F

F

V

I

G

G

-

A

-

P

E

E

I

M

N

N

I

I

L

L

N

E

A

V

V

S

I

V

A

-

N

-

H

-

G

G

D

D

G

G

L

Y

Y

L

D

E

A

G

V

R

I

G

E

F

G

R

V

I

T

E

F

L

P

P

I

Q

R

M

S

D

Q

L

R

L

T

K

F

D

A

Y

P

V

G

G

D

K

K

T

V

V

N

L

V

F

L

G

L

I

R

R

P

P

E

E

D

H

L

M

R

T

V

V

Y

E

T

G

L

V

T

H

E

M

D

K

R

R

P

T

A

A

G

-

P

-

H

R

L

L

T

I

G

G

R

K

I

V

E

D

binding adenosine-5'-triphosphate: F16 (= F17), F19 (≠ T20), S41 (= S42), G42 (= G43), G44 (= G45), K45 (= K46), T46 (= T47), T47 (= T48)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
42% identity, 77% coverage: 8:289/368 of query aligns to 4:288/369 of P19566

query
sites
P19566

I

V

E

Q

L

L

R

R

G

N

V

V

T

T

K

K

N

A

F

W

E

G

D

D

T

V

C

V

A

V

L

S

D

K

N

D

I

I

D

N

L

L

E

D

I

I

R

H

N

D

G

G

E

E

F

F

L

V

T

V

L

F

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

I

L

L

L

R

R

L

M

I

I

S

A

G

G

F

L

E

E

K

T

P

I

D

T

A

S

G

G

V

D

I

L

T

F

L

I

K

G

G

E

Q

T

R

R

M

M

D

N

D

D

A

I

P

P

E

A

A

E

R

R

Q

G

V

V

N

G

T

M

V

V

F

F

Q

Q

N

S

Y

Y

A

A

L
|
L

F

Y

P

P

H

H

M

L

S

S

V

V

R

A

E

E

N

N

V

M

G

S

F

F

G

G

L

L