SitesBLAST

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
43% identity, 62% coverage: 17:234/354 of query aligns to 17:234/374 of 2awnB

query
sites
2awnB

V
|
V

L

S

H

K

D

D

V

I

N

N

L

L

T

D

A

I

A

H

A

E

G

G

E

E

V

F

V

V

C

V

L

F

V

V

G

G

P

P

S
|
S

G
|
G

V
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

C

M

I

I

A

A

G

G

L

L

D

E

A

T

P

I

D

T

E

S

G

G

T

D

I

L

R

F

V

I

-

G

-

E

-

K

-

R

-

M

-

N

-

D

T

T

P

P

Q

A

G

E

H

R

G

G

G

V

G

G

V

-

L

M

V

V

F

F

Q

Q

D

S

Y

Y

L

A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

F

A

E

E

N

N

V

M

A

S

F

F

G

G

P

L

R

K

A

L

R

A

G

G

V

A

R

K

G

K

A

E

A

V

L

I

K

N

E

Q

R

R

V

V

H

N

T

Q

M

V

L

A

R

E

A

V

F

L

R

Q

L

L

D

-

T

-

D

-

L

-

A

A

H

H

M

L

A

L

S

D

R

R

Y

K

P

P

A

K

Q

A

L

L

S

S

A

G

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

C

A

D

E

P

P

A

S

V

V

L

F

L

L

D

D

E

E

P

P

F

L

A

S

N

N

L

L

D

D

R

A

G

A

L

L

R

R

G

V

E

Q

M

M

A

R

A

I

F

E

V

I

R

S

D

R

V

L

V

H

R

K

R

R

F

L

G

G

V

R

A

T

T

M

V

I

T

Y

V

V

T

T

H

H

D

D

L

Q

E

V

E

E

A

A

F

M

A

T

I

L

G

A

D

D

R

K

L

I

G

V

V

V

M

L

L

D

G

A

G

G

T

R

L

V

A

A

Q

Q

L

V

A

G

P

K

P

P

L

L

D

E

V

L

Y

Y

R

H

H

Y

P

P

A

A

D

D

E

R

A

F

T

V

A

A

R

G

F

F

L

I

G

G

binding adenosine-5'-diphosphate: V17 (= V17), S37 (= S37), G38 (= G38), C39 (≠ V39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
43% identity, 62% coverage: 17:234/354 of query aligns to 15:232/367 of 1q12A

query
sites
1q12A

V
|
V

L

S

H

K

D

D

V

I

N

N

L

L

T

D

A

I

A

H

A

E

G

G

E

E

V

F

V

V

C

V

L

F

V

V

G

G

P

P

S
|
S

G
|
G

V
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

C

M

I

I

A

A

G

G

L

L

D

E

A

T

P

I

D

T

E

S

G

G

T

D

I

L

R

F

V

I

-

G

-

E

-

K

-

R

-

M

-

N

-

D

T

T

P

P

Q

A

G

E

H

R

G

G

G

V

G

G

V

-

L

M

V

V

F

F

Q

Q

D

S

Y

Y

L

A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

F

A

E

E

N

N

V

M

A

S

F

F

G

G

P

L

R

K

A

L

R

A

G

G

V

A

R

K

G

K

A

E

A

V

L

I

K

N

E

Q

R

R

V

V

H

N

T

Q

M

V

L

A

R

E

A

V

F

L

R

Q

L

L

D

-

T

-

D

-

L

-

A

A

H

H

M

L

A

L

S

D

R
|
R

Y

K

P

P

A
x
K

Q
x
A

L
|
L

S
|
S

A
x
G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

C

A

D

E

P

P

A

S

V

V

L

F

L

L

D

D

E

E

P

P

F

L

A

S

N
|
N

L

L

D

D

R

A

G

A

L

L

R

R

G

V

E

Q

M

M

A

R

A

I

F

E

V

I

R

S

D

R

V

L

V

H

R

K

R

R

F

L

G

G

V

R

A

T

T

M

V

I

T

Y

V

V

T

T

H
|
H

D

D

L

Q

E

V

E

E

A

A

F

M

A

T

I

L

G

A

D

D

R

K

L

I

G

V

V

V

M

L

L

D

G

A

G

G

T

R

L

V

A

A

Q

Q

L

V

A

G

P

K

P

P

L

L

D

E

V

L

Y

Y

R

H

H

Y

P

P

A

A

D

D

E

R

A

F

T

V

A

A

R

G

F

F

L

I

G

G

binding adenosine-5'-triphosphate: V15 (= V17), S35 (= S37), G36 (= G38), C37 (≠ V39), G38 (= G40), K39 (= K41), S40 (≠ T42), T41 (= T43), R126 (= R128), K129 (≠ A131), A130 (≠ Q132), L131 (= L133), S132 (= S134), G133 (≠ A135), G134 (= G136), Q135 (= Q137), N160 (= N162), H189 (= H191)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 10

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
43% identity, 62% coverage: 17:234/354 of query aligns to 18:235/371 of P68187

query
sites
P68187

V

V

L

S

H

K

D

D

V

I

N

N

L

L

T

D

A

I

A

H

A

E

G

G

E

E

V

F

V

V

C

V

L

F

V

V

G

G

P

P

S

S

G

G

V

C

G

G

K

K

T

S

T

T

L

L

R

R

C

M

I

I

A

A

G

G

L

L

D

E

A

T

P

I

D

T

E

S

G

G

T

D

I

L

R

F

V

I

-

G

-

E

-

K

-

R

-

M

-

N

-

D

T

T

P

P

Q

A

G

E

H

R

G

G

G

V

G

G

V

-

L

M

V

V

F

F

Q

Q

D

S

Y

Y

L
x
A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

F

A

E

E

N

N

V

M

A

S

F

F

G

G

P

L

R

K

A

L

R

A

G

G

V

A

R
x
K

G

K

A

E

A

V

L

I

K

N

E

Q

R

R

V
|
V

H

N

T

Q

M
x
V

L

A

R
x
E

A

V

F

L

R

Q

L

L

D

-

T

-

D

-

L

-

A
|
A

H

H

M

L

A

L

S

D

R

R

Y

K

P

P

A

K

Q

A

L

L

S

S

A

G

G
|
G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

C

A

D

E

P

P

A

S

V

V

L

F

L

L

D
|
D

E

E

P

P

F

L

A

S

N

N

L

L

D

D

R

A

G

A

L

L

R

R

G

V

E

Q

M

M

A

R

A

I

F

E

V

I

R

S

D

R

V

L

V

H

R

K

R

R

F

L

G

G

V

R

A

T

T

M

V

I

T

Y

V

V

T

T

H

H

D

D

L

Q

E

V

E

E

A

A

F

M

A

T

I

L

G

A

D

D

R

K

L

I

G

V

V

V

M

L

L

D

G

A

G

G

T

R

L

V

A

A

Q

Q

L

V

A

G

P

K

P

P

L

L

D

E

V

L

Y

Y

R

H

H

Y

P

P

A

A

D

D

E
x
R

A

F

T

V

A

A

R

G

F

F

L

I

G

G

A85 (≠ L79) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ R100) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V108) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ M111) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ R113) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (= A123) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G136) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D157) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ E227) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

2awoA Crystal structure of the adp-mg-bound e. Coli malk (crystallized with adp-mg) (see paper)
43% identity, 62% coverage: 17:234/354 of query aligns to 17:234/372 of 2awoA

query
sites
2awoA

V
|
V

L

S

H

K

D

D

V

I

N

N

L

L

T

D

A

I

A

H

A

E

G

G

E

E

V

F

V

V

C

V

L

F

V

V

G

G

P

P

S
|
S

G
|
G

V
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

C

M

I

I

A

A

G

G

L

L

D

E

A

T

P

I

D

T

E

S

G

G

T

D

I

L

R

F

V

I

-

G

-

E

-

K

-

R

-

M

-

N

-

D

T

T

P

P

Q

A

G

E

H

R

G

G

G

V

G

G

V

-

L

M

V

V

F

F

Q

Q

D

S

Y

Y

L

A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

F

A

E

E

N

N

V

M

A

S

F

F

G

G

P

L

R

K

A

L

R

A

G

G

V

A

R

K

G

K

A

E

A

V

L

I

K

N

E

Q

R

R

V

V

H

N

T

Q

M

V

L

A

R

E

A

V

F

L

R

Q

L

L

D

-

T

-

D

-

L

-

A

A

H

H

M

L

A

L

S

D

R

R

Y

K

P

P

A

K

Q

A

L

L

S

S

A

G

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

C

A

D

E

P

P

A

S

V

V

L

F

L

L

D
|
D

E

E

P

P

F

L

A

S

N

N

L

L

D

D

R

A

G

A

L

L

R

R

G

V

E

Q

M

M

A

R

A

I

F

E

V

I

R

S

D

R

V

L

V

H

R

K

R

R

F

L

G

G

V

R

A

T

T

M

V

I

T

Y

V

V

T

T

H

H

D

D

L

Q

E

V

E

E

A

A

F

M

A

T

I

L

G

A

D

D

R

K

L

I

G

V

V

V

M

L

L

D

G

A

G

G

T

R

L

V

A

A

Q

Q

L

V

A

G

P

K

P

P

L

L

D

E

V

L

Y

Y

R

H

H

Y

P

P

A

A

D

D

E

R

A

F

T

V

A

A

R

G

F

F

L

I

G

G

binding adenosine-5'-diphosphate: V17 (= V17), S37 (= S37), G38 (= G38), C39 (≠ V39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43)
binding magnesium ion: S42 (≠ T42), D157 (= D157)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
43% identity, 62% coverage: 17:234/354 of query aligns to 17:234/371 of 3puyA

query
sites
3puyA

V
|
V

L

S

H

K

D

D

V

I

N

N

L

L

T

D

A

I

A

H

A

E

G

G

E

E

V

F

V

V

C

V

L

F

V

V

G

G

P
|
P

S
|
S

G
|
G

V
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

C

M

I

I

A

A

G

G

L

L

D

E

A

T

P

I

D

T

E

S

G

G

T

D

I

L

R

F

V

I

-

G

-

E

-

K

-

R

-

M

-

N

-

D

T

T

P

P

Q

A

G

E

H

R

G

G

G

V

G

G

V

-

L

M

V

V

F

F

Q
|
Q

D

S

Y

Y

L

A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

F

A

E

E

N

N

V

M

A

S

F

F

G

G

P

L

R

K

A

L

R

A

G

G

V

A

R

K

G

K

A

E

A

V

L

I

K

N

E

Q

R

R

V

V

H

N

T

Q

M

V

L

A

R

E

A

V

F

L

R

Q

L

L

D

-

T

-

D

-

L

-

A

A

H

H

M

L

A

L

S

D

R
|
R

Y

K

P

P

A

K

Q
x
A

L

L

S
|
S

A
x
G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

C

A

D

E

P

P

A

S

V

V

L

F

L

L

D
|
D

E
|
E

P

P

F

L

A

S

N

N

L

L

D

D

R

A

G

A

L

L

R

R

G

V

E

Q

M

M

A

R

A

I

F

E

V

I

R

S

D

R

V

L

V

H

R

K

R

R

F

L

G

G

V

R

A

T

T

M

V

I

T

Y

V

V

T

T

H
|
H

D

D

L

Q

E

V

E

E

A

A

F

M

A

T

I

L

G

A

D

D

R

K

L

I

G

V

V

V

M

L

L

D

G

A

G

G

T

R

L

V

A

A

Q

Q

L

V

A

G

P

K

P

P

L

L

D

E

V

L

Y

Y

R

H

H

Y

P

P

A

A

D

D

E

R

A

F

T

V

A

A

R

G

F

F

L

I

G

G

binding phosphoaminophosphonic acid-adenylate ester: V17 (= V17), P36 (= P36), S37 (= S37), G38 (= G38), C39 (≠ V39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), Q81 (= Q76), R128 (= R128), A132 (≠ Q132), S134 (= S134), G135 (≠ A135), G136 (= G136), Q137 (= Q137), E158 (= E158), H191 (= H191)
binding magnesium ion: S42 (≠ T42), Q81 (= Q76), D157 (= D157)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 12

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
43% identity, 62% coverage: 17:234/354 of query aligns to 17:234/371 of 3puxA

query
sites
3puxA

V
|
V

L

S

H

K

D

D

V

I

N

N

L

L

T

D

A

I

A

H

A

E

G

G

E

E

V

F

V

V

C

V

L

F

V

V

G

G

P

P

S
|
S

G
|
G

V
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

C

M

I

I

A

A

G

G

L

L

D

E

A

T

P

I

D

T

E

S

G

G

T

D

I

L

R

F

V

I

-

G

-

E

-

K

-

R

-

M

-

N

-

D

T

T

P

P

Q

A

G

E

H

R

G

G

G

V

G

G

V

-

L

M

V

V

F

F

Q
|
Q

D

S

Y

Y

L

A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

F

A

E

E

N

N

V

M

A

S

F

F

G

G

P

L

R

K

A

L

R

A

G

G

V

A

R

K

G

K

A

E

A

V

L

I

K

N

E

Q

R

R

V

V

H

N

T

Q

M

V

L

A

R

E

A

V

F

L

R

Q

L

L

D

-

T

-

D

-

L

-

A

A

H

H

M

L

A

L

S

D

R
|
R

Y

K

P

P

A

K

Q
x
A

L
|
L

S
|
S

A
x
G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

C

A

D

E

P

P

A

S

V

V

L

F

L

L

D
|
D

E
|
E

P

P

F

L

A

S

N

N

L

L

D

D

R

A

G

A

L

L

R

R

G

V

E

Q

M

M

A

R

A

I

F

E

V

I

R

S

D

R

V

L

V

H

R

K

R

R

F

L

G

G

V

R

A

T

T

M

V

I

T

Y

V

V

T

T

H
|
H

D

D

L

Q

E

V

E

E

A

A

F

M

A

T

I

L

G

A

D

D

R

K

L

I

G

V

V

V

M

L

L

D

G

A

G

G

T

R

L

V

A

A

Q

Q

L

V

A

G

P

K

P

P

L

L

D

E

V

L

Y

Y

R

H

H

Y

P

P

A

A

D

D

E

R

A

F

T

V

A

A

R

G

F

F

L

I

G

G

binding adenosine-5'-diphosphate: V17 (= V17), S37 (= S37), G38 (= G38), C39 (≠ V39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), R128 (= R128), A132 (≠ Q132), L133 (= L133), S134 (= S134), Q137 (= Q137)
binding beryllium trifluoride ion: S37 (= S37), G38 (= G38), K41 (= K41), Q81 (= Q76), S134 (= S134), G135 (≠ A135), G136 (= G136), E158 (= E158), H191 (= H191)
binding magnesium ion: S42 (≠ T42), Q81 (= Q76), D157 (= D157)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
43% identity, 62% coverage: 17:234/354 of query aligns to 17:234/371 of 3puwA

query
sites
3puwA

V
|
V

L

S

H

K

D

D

V

I

N

N

L

L

T

D

A

I

A

H

A

E

G

G

E

E

V

F

V

V

C

V

L

F

V

V

G

G

P

P

S
|
S

G
|
G

V
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

C

M

I

I

A

A

G

G

L

L

D

E

A

T

P

I

D

T

E

S

G

G

T

D

I

L

R

F

V

I

-

G

-

E

-

K

-

R

-

M

-

N

-

D

T

T

P

P

Q

A

G

E

H

R

G

G

G

V

G

G

V

-

L

M

V

V

F

F

Q
|
Q

D

S

Y

Y

L

A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

F

A

E

E

N

N

V

M

A

S

F

F

G

G

P

L

R

K

A

L

R

A

G

G

V

A

R

K

G

K

A

E

A

V

L

I

K

N

E

Q

R

R

V

V

H

N

T

Q

M

V

L

A

R

E

A

V

F

L

R

Q

L

L

D

-

T

-

D

-

L

-

A

A

H

H

M

L

A

L

S

D

R
|
R

Y

K

P

P

A

K

Q
x
A

L

L

S
|
S

A
x
G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

C

A

D

E

P

P

A

S

V

V

L

F

L

L

D

D

E
|
E

P

P

F

L

A

S

N
|
N

L

L

D

D

R

A

G

A

L

L

R

R

G

V

E

Q

M

M

A

R

A

I

F

E

V

I

R

S

D

R

V

L

V

H

R

K

R

R

F

L

G

G

V

R

A

T

T

M

V

I

T

Y

V

V

T

T

H
|
H

D

D

L

Q

E

V

E

E

A

A

F

M

A

T

I

L

G

A

D

D

R

K

L

I

G

V

V

V

M

L

L

D

G

A

G

G

T

R

L

V

A

A

Q

Q

L

V

A

G

P

K

P

P

L

L

D

E

V

L

Y

Y

R

H

H

Y

P

P

A

A

D

D

E

R

A

F

T

V

A

A

R

G

F

F

L

I

G

G

binding adenosine-5'-diphosphate: V17 (= V17), S37 (= S37), G38 (= G38), C39 (≠ V39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), R128 (= R128), A132 (≠ Q132), S134 (= S134), Q137 (= Q137)
binding tetrafluoroaluminate ion: S37 (= S37), G38 (= G38), K41 (= K41), Q81 (= Q76), S134 (= S134), G135 (≠ A135), G136 (= G136), E158 (= E158), N162 (= N162), H191 (= H191)
binding magnesium ion: S42 (≠ T42), Q81 (= Q76)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
36% identity, 83% coverage: 17:310/354 of query aligns to 18:315/369 of P19566

query
sites
P19566

V

V

L

S

H

K

D

D

V

I

N

N

L

L

T

D

A

I

A

H

A

D

G

G

E

E

V

F

V

V

C

V

L

F

V

V

G

G

P

P

S

S

G

G

V

C

G

G

K

K

T

S

T

T

L

L

R

R

C

M

I

I

A

A

G

G

L

L

D

E

A

T

P

I

D

T

E

S

G

G

T

D

I

L

R

F

V

I

-

G

-

E

-

T

-

R

-

M

-

N

-

D

T

I

P

P

Q

A

G

E

H

R

G

G

G

V

G

G

V

-

L

M

V

V

F

F

Q

Q

D

S

Y

Y

L

A

L
|
L

F

Y

P

P

H

H

L

L

S

S

V

V

F

A

E

E

N

N

V

M

A

S

F

F

G

G

P

L

R

K

A

L

R

A

G

G

V

A

R

K

G

K

A

E

A

V

L

M

K

N

E

Q

R

R

V

V

H

N

T

Q

M

V

L

A

R

E

A

V

F

L

R

Q

L

L

D

-

T

-

D

-

L

-

A

A

H

H

M

L

A

L

S

E

R

R

Y

K

P

P

A

K

Q

A

L

L

S

S

A

G

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

C

A

D

E

P

P

A

R

V

V

L

F

L

L

D

D

E

E

P
|
P

F

L

A

S

N

N

L

L

D
|
D

R

A

G

A

L

L

R

R

G

V

E

Q

M

M

A

R

A

I

F

E

V

I

R

S

D

R

V

L

V

H

R

K

R

R

F

L

G

G

V

R

A

T

T

M

V

I

T

Y

V

V

T

T

H

H

D

D

L

Q

E

V

E

E

A

A

F

M

A

T

I

L

G

A

D

D

R

K

L

I

G

V

V

V

M

L

L

D

G

A

G

G

T

R

L

V

A

A

Q

Q

L

V

A

G

P

K

P

P

L

L

D

E

V

L

Y

Y

R

H

H

Y

P

P

A

A

D

D

E

R

A

F

T

V

A

A

R

G

F

F

L

I

G

G

-

S

-

P

-

K

-

M

-

N

-

F

-

L

P

P

V

V

T

K

V

V

L

-

D

T

E

A

T

T

T

A

R

I

R

E

T

Q

L

V

G

Q

I

V

D

E

T

L

P

P

P

N

A

R

A

Q

T

I

A

W

C

L

A

P

D

V

T

E

M

S

Q

R

G

G

L

V

R

Q

L

V

Y

G

R

A

P

N

E

M

A

S

L

L

A

G

V

I

R

R

P

P

W

E

A

H

D

L

G

L

P

P

A

S

V

D

L

I

V

A

S

D

A

V

R

T

F

L

T

E

G

G

Q

E

V

V

M

Q

Q

-

L

-

L

V

D
x
E

V

Q

E

L

G

G

Q

H

E

E

L

T

L

Q

V

I

H

H

L86 (= L80) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P159) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D164) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
E306 (≠ D301) mutation to K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
40% identity, 63% coverage: 18:240/354 of query aligns to 21:248/353 of 1oxvD

query
sites
1oxvD

L

L

H

D

D

N

V

V

N

N

L

I

T

N

A

I

A

E

A

N

G

G

E

E

V

R

V

F

C

G

L

I

V

L

G

G

P
|
P

S
|
S

G
|
G

V
x
A

G
|
G

K
|
K

T
|
T

L

F

L

M

R

R

C

I

I

I

A

A

G

G

L

L

D

D

A

V

P

P

D

S

E

T

G

G

T

E

I

L

R

Y

-

F

-

D

-

R

-

L

-

V

-

A

-

S

-

N

-

G

-

K

-

L

V

I

T

V

P

P

Q

E

G

D

H

R

G

K

G

I

G

G

V

-

L

M

V

V

F

F

Q
|
Q

D

T

Y

W

L

A

L

L

F

Y

P

P

H

N

L

L

S

T

V

A

F

F

E

E

N

N

V

I

A

A

F

F

G

P

P

L

R

T

A

N

R

M

G

K

V

M

R

S

G

K

A

E

L

I

K

R

E

K

R

R

V

V

H

E

T

E

M

V

L

A

R

K

A

I

F

L

R

-

L

-

D

-

T

-

D

-

D

D

L

I

A

H

H

H

M

V

A

L

S

N

R

H

Y

F

P

P

A

R

Q

E

L

L

S

S

A

G

G

G

Q

Q

R

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

V

V

C

K

D

D

P

P

A

S

V

L

L

L

D

D

E
|
E

P

P

F

F

A

S

N

N

L

L

D

D

R

A

G

R

L

M

R

R

G

D

E

S

M

A

A

R

A

A

F

L

V

V

R

K

D

E

V

V

V

Q

R

S

R

R

F

L

G

G

V

V

A

T

T

L

V

L

T

V

V

V

T

S

H

H

D

D

L

P

E

A

E

D

A

I

F

F

A

A

I

I

G

A

D

D

R

R

L

V

G

G

V

V

M

L

L

V

G

K

G

G

T

K

L

L

A

V

Q

Q

L

V

A

G

P

K

P

P

L

E

D

D

V

L

Y

Y

R

D

H

N

P

P

A

V

D

S

E

I

A

Q

T

V

A

A

R

S

F

L

L

I

G

G

P

E

V

I

T

N

V

E

L

L

D

E

binding phosphoaminophosphonic acid-adenylate ester: P39 (= P36), S40 (= S37), G41 (= G38), A42 (≠ V39), G43 (= G40), K44 (= K41), T45 (= T42), T46 (= T43), Q89 (= Q76), E166 (= E158)
binding magnesium ion: T45 (= T42), Q89 (= Q76)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13, 18, 20

1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
40% identity, 63% coverage: 18:240/354 of query aligns to 21:248/353 of 1oxvA

query
sites
1oxvA

L

L

H

D

D

N

V

V

N

N

L

I

T

N

A

I

A

E

A

N

G

G

E

E

V

R

V

F

C

G

L

I

V

L

G

G

P
|
P

S
|
S

G
|
G

V
x
A

G
|
G

K
|
K

T
|
T

L

F

L

M

R

R

C

I

I

I

A

A

G

G

L

L

D

D

A

V

P

P

D

S

E

T

G

G

T

E

I

L

R

Y

-

F

-

D

-

R

-

L

-

V

-

A

-

S

-

N

-

G

-

K

-

L

V

I

T

V

P

P

Q

E

G

D

H

R

G

K

G

I

G

G

V

-

L

M

V

V

F

F

Q

Q

D

T

Y

W

L

A

L

L

F

Y

P

P

H

N

L

L

S

T

V

A

F

F

E

E

N

N

V

I

A

A

F

F

G

P

P

L

R

T

A

N

R

M

G

K

V

M

R

S

G

K

A

E

L

I

K

R

E

K

R

R

V

V

H

E

T

E

M

V

L

A

R

K

A

I

F

L

R

-

L

-

D

-

T

-

D

-

D

D

L

I

A

H

H

H

M

V

A

L

S

N

R

H

Y

F

P

P

A

R

Q

E

L

L

S

S

A

G

G

G

Q

Q

R

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

V

V

C

K

D

D

P

P

A

S

V

L

L

L

D

D

E
|
E

P

P

F

F

A

S

N

N

L

L

D

D

R

A

G

R

L

M

R

R

G

D

E

S

M

A

A

R

A

A

F

L

V

V

R

K

D

E

V

V

V

Q

R

S

R

R

F

L

G

G

V

V

A

T

T

L

V

L

T

V

V

V

T

S

H

H

D

D

L

P

E

A

E

D

A

I

F

F

A

A

I

I

G

A

D

D

R

R

L

V

G

G

V

V

M

L

L

V

G

K

G

G

T

K

L

L

A

V

Q

Q

L

V

A

G

P

K

P

P

L

E

D

D

V

L

Y

Y

R

D

H

N

P

P

A

V

D

S

E

I

A

Q

T

V

A

A

R

S

F

L

L

I

G

G

P

E

V

I

T

N

V

E

L

L

D

E

binding phosphoaminophosphonic acid-adenylate ester: P39 (= P36), S40 (= S37), G41 (= G38), A42 (≠ V39), G43 (= G40), K44 (= K41), T45 (= T42), T46 (= T43), E166 (= E158)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13, 18, 20

1oxuA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
40% identity, 63% coverage: 18:240/354 of query aligns to 21:248/353 of 1oxuA

query
sites
1oxuA

L

L

H

D

D

N

V

V

N

N

L

I

T

N

A

I

A

E

A

N

G

G

E

E

V

R

V

F

C

G

L

I

V

L

G

G

P

P

S
|
S

G
|
G

V
x
A

G
|
G

K
|
K

T
|
T

L

F

L

M

R

R

C

I

I

I

A

A

G

G

L

L

D

D

A

V

P

P

D

S

E

T

G

G

T

E

I

L

R

Y

-

F

-

D

-

R

-

L

-

V

-

A

-

S

-

N

-

G

-

K

-

L

V

I

T

V

P

P

Q

E

G

D

H

R

G

K

G

I

G

G

V

-

L

M

V

V

F

F

Q

Q

D

T

Y

W

L

A

L

L

F

Y

P

P

H

N

L

L

S

T

V

A

F

F

E

E

N

N

V

I

A

A

F

F

G

P

P

L

R

T

A

N

R

M

G

K

V

M

R

S

G

K

A

E

L

I

K

R

E

K

R

R

V

V

H

E

T

E

M

V

L

A

R

K

A

I

F

L

R

-

L

-

D

-

T

-

D

-

D

D

L

I

A

H

H

H

M

V

A

L

S

N

R

H

Y

F

P

P

A

R

Q

E

L

L

S

S

A

G

G

G

Q

Q

R

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

V

V

C

K

D

D

P

P

A

S

V

L

L

L

D

D

E

E

P

P

F

F

A

S

N

N

L

L

D

D

R

A

G

R

L

M

R

R

G

D

E

S

M

A

A

R

A

A

F

L

V

V

R

K

D

E

V

V

V

Q

R

S

R

R

F

L

G

G

V

V

A

T

T

L

V

L

T

V

V

V

T

S

H

H

D

D

L

P

E

A

E

D

A

I

F

F

A

A

I

I

G

A

D

D

R

R

L

V

G

G

V

V

M

L

L

V

G

K

G

G

T

K

L

L

A

V

Q

Q

L

V

A

G

P

K

P

P

L

E

D

D

V

L

Y

Y

R

D

H

N

P

P

A

V

D

S

E

I

A

Q

T

V

A

A

R

S

F

L

L

I

G

G

P

E

V

I

T

N

V

E

L

L

D

E

binding adenosine-5'-diphosphate: S40 (= S37), G41 (= G38), A42 (≠ V39), G43 (= G40), K44 (= K41), T45 (= T42), T46 (= T43)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 13, 20

Q97UY8 Glucose import ATP-binding protein GlcV; EC 7.5.2.- from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
40% identity, 63% coverage: 18:240/354 of query aligns to 21:248/353 of Q97UY8

query
sites
Q97UY8

L

L

H

D

D

N

V

V

N

N

L

I

T

N

A

I

A

E

A

N

G

G

E

E

V

R

V

F

C

G

L

I

V

L

G

G

P

P

S

S

G

G

V

A

G

G

K

K

T

T

L

F

L

M

R

R

C

I

I

I

A

A

G

G

L

L

D

D

A

V

P

P

D

S

E

T

G

G

T

E

I

L

R

Y

-

F

-

D

-

R

-

L

-

V

-

A

-

S

-

N

-

G

-

K

-

L

V

I

T

V

P

P

Q

E

G

D

H

R

G

K

G

I

G

G

V

-

L

M

V

V

F

F

Q

Q

D

T

Y

W

L

A

L

L

F

Y

P

P

H

N

L

L

S

T

V

A

F

F

E

E

N

N

V

I

A

A

F

F

G

P

P

L

R

T

A

N

R

M

G

K

V

M

R

S

G

K

A

E

L

I

K

R

E

K

R

R

V

V

H

E

T

E

M

V

L

A

R

K

A

I

F

L

R

-

L

-

D

-

T

-

D

-

D

D

L

I

A

H

H

H

M

V

A

L

S

N

R

H

Y

F

P

P

A

R

Q

E

L

L

S
|
S

A

G

G
|
G

Q

Q

R

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

V

V

C

K

D

D

P

P

A

S

V

L

L

L

D

D

E
|
E

P

P

F

F

A

S

N

N

L

L

D

D

R

A

G

R

L

M

R

R

G

D

E

S

M

A

A

R

A

A

F

L

V

V

R

K

D

E

V

V

V

Q

R

S

R

R

F

L

G

G

V

V

A

T

T

L

V

L

T

V

V

V

T

S

H

H

D

D

L

P

E

A

E

D

A

I

F

F

A

A

I

I

G

A

D

D

R

R

L

V

G

G

V

V

M

L

L

V

G

K

G

G

T

K

L

L

A

V

Q

Q

L

V

A

G

P

K

P

P

L

E

D

D

V

L

Y

Y

R

D

H

N

P

P

A

V

D

S

E

I

A

Q

T

V

A

A

R

S

F

L

L

I

G

G

P

E

V

I

T

N

V

E

L

L

D

E

S142 (= S134) mutation to A: Decrease in ATPase activity. Can form dimers.
G144 (= G136) mutation to A: Loss of ATPase activity. Cannot form dimers. Forms an active heterodimer; when associated with A-166.
E166 (= E158) mutation to A: Loss of ATPase activity. Can form dimers in the presence of ATP-Mg(2+). Forms an active heterodimer; when associated with A-144.; mutation to Q: Strong decrease in ATPase activity. Can form dimers in the presence of ATP alone, without Mg(2+).

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
35% identity, 91% coverage: 14:334/354 of query aligns to 16:345/393 of P9WQI3

query
sites
P9WQI3

G

G

R

H

E

T

V

A

L

V

H

R

D

D

V

L

N

N

L

L

T

T

A

I

A

A

A

D

G

G

E

E

V

F

V

L

C

I

L

L

V

V

G

G

P

P

S

S

G

G

V

C

G

G

K

K

T

T

L

T

L

L

R

N

C

M

I

I

A

A

G

G

L

L

D

E

A

D

P

I

D

S

E

S

G

G

T

E

I

L

R

R

V

I

T

A

P

G

P

E

Q

R

G

V

H

N

G

E

G

K

G

A

-

P

-

K

-

D

-

R

-

D

V

I

V

A

L

M

V

V

F

F

Q

Q

D

S

Y

Y

L

A

L

L

F

Y

P

P

H

H

L

M

S

T

V

V

F

R

E

Q

N

N

V

I

A

A

F

F

G

P

P

L

R

T

A

L

R

A

G

K

V

M

R

R

G

K

A

A

A

D

L

I

K

A

E

Q

R

K

V

V

H

S

T

E

M

T

L

A

R

K

A

I

F

L

R

-

L

-

D

-

T

-

D

-

D

D

L

L

A

T

H

N

M

L

A

L

S

D

R

R

Y

K

P

P

A

S

Q

Q

L

L

S

S

A

G

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

V

V

C

R

D

H

P

P

A

K

V

A

L

F

L

L

L

M

D

D

E

E

P

P

F

L

A

S

N

N

L

L

D

D

R

A

G

K

L

L

R

R

G

V

E

Q

M

M

A

R

A

G

F

E

V

I

R

A

D

Q

V

L

V

Q

R

R

F

L

G

G

V

T

A

T

T

T

V

V

T

Y

V

V

T

T

H
|
H

D

D

L

Q

E

T

E

E

A

A

F

M

A

T

I

L

G

G

D

D

R

R

L

V

G

V

V

V

M

M

L

Y

G

G

T

I

L

A

A

Q

Q

Q

L

I

A

G

P

T

P

P

L

E

D

E

V

L

Y

Y

R

E

H

R

P

P

A

A

D

N

E

L

A

F

T

V

A

A

R

G

F

F

L

I

G

G

P

S

V

P

T

A

V

M

L

N

D

F

E

F

T

P

T

A

R

R

R

L

T

T

L

A

G

I

I

G

D

L

T

T

P

L

P

P

A

F

A

G

A

E

T

V

A

T

C

L

A

A

D

P

T

E

M

V

Q

Q

G

G

L

V

R

I

L

A

Y

A

R

H

P

P

E

K

-

P

-

E

-

N

-

V

A

I

L

V

A

G

V

V

R

R

P

P

W

E

A

H

D

I

G

Q

P

D

A

A

V

A

L

L

V

I

S

D

A

A

R

Y

-

Q

-

R

-

I

-

R

-

A

F

L

T

T

G

F

Q

Q

V

V

M

K

Q

V

L

N

L

L

L

V

D

E

V

S

E

L

G

G

Q

A

E

D

L

K

L

Y

V

L

H

Y

T

F

L

T

D

T

D

E

A

S

P

P

P

A

T

V

G

H

T

S

R

V

L

Q

C

L

V

D

S

E

L

L

R

A

E

E

I

V

P

E

G

G

M

E

T

S

S

A

H193 (= H191) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
40% identity, 64% coverage: 18:242/354 of query aligns to 22:254/375 of 2d62A

query
sites
2d62A

L

V

H

K

D

D

V

L

N

S

L

L

T

E

A

I

A

K

A

D

G

G

E

E

V

F

V

L

C

V

L

L

V

L

G

G

P
|
P

S
|
S

G
|
G

V
x
C

G
|
G

K
|
K

T
|
T

L

T

L

L

R

R

C

M

I

I

A

A

G

G

L

L

D

E

A

E

P

P

D

T

E

R

G

G

T

Q

I

I

R

Y

V

I

-

E

-

D

-

N

-

L

-

V

-

A

-

D

-

P

-

E

-

K

-

G

-

V

-

F

T

V

P

P

Q

K

G

E

H

R

G

D

G

-

V

V

V

A

L

M

V

V

F

F

Q

Q

D

S

Y

Y

L

A

L

L

F

Y

P

P

H

H

L

M

S

T

V

V

F

Y

E

D

N

N

V

I

A

A

F

F

G

P

P

L

R

K

A

L

R

R

G

K

V

V

R

P

G

K

A

Q

A

E

L

I

K

D

E

K

R

R

V

V

H

R

T

E

M

V

L

A

R

E

A

M

F

L

R

-

L

-

D

-

T

-

D

-

D

G

L

L

A

T

H

E

M

L

A

L

S

N

R

R

Y

K

P

P

A

R

Q

E

L

L

S

S

A

G

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

L

A

G

R

R

A

A

L

I

V

I

C

R

D

R

P

P

A

K

V

V

L

F

L

L

L

M

D

D

E

E

P

P

F

L

A

S

N

N

L

L

D

D

R

A

G

K

L

L

R

R

G

V

E

K

M

M

A

R

A

A

F

E

V

L

R

K

D

K

V

L

V

Q

R

R

R

Q

F

L

G

G

V

V

A

T

T

T

V

I

T

Y

V

V

T

T

H

H

D

D

L

Q

E

V

E

E

A

A

F

M

A

T

I

M

G

G

D

D

R

R

L

I

G

A

V

V

M

M

L

N

G

K

G

G

T

E

L

L

A

Q

Q

Q

L

V

A

G

P

T

P

P

L

D

D

E

V

V

Y

Y

R

Y

H

K

P

P

A

V

D

N

E

T

A

F

T

V

A

A

R

G

F

F

L

I

G

G

-

S

-

P

P

P

V

M

T

N

V

F

L

L

D

D

E

A

T

T

binding pyrophosphate 2-: P40 (= P36), S41 (= S37), G42 (= G38), C43 (≠ V39), G44 (= G40), K45 (= K41), T46 (= T42), T47 (= T43)

2awnC Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
43% identity, 62% coverage: 17:234/354 of query aligns to 10:204/344 of 2awnC

query
sites
2awnC

V
|
V

L

S

H

K

D

D

V

I

N

N

L

L

T

D

A

I

A

H

A

E

G

G

E

E

V

F

V

V

C

V

L

F

V

V

G

G

P

P

S
|
S

G
|
G

V
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

C

M

I

I

A

A

G

G

L

L

D

-

A

-

P

-

D

-

E

-

G

-

T

-

I

-

R

-

V

-

T

-

P

-

Q

-

G

-

H

-

G

-

G

G

V

V

V

G

L

M

V

V

F

F

Q

Q

D

S

Y

Y

L

A

L

L

F

Y

P

P

H

H

L

L

S

S

V

V

F

A

E

E

N

N

V

M

A

S

F

F

G

G

P

L

R

K

A

L

R

A

G

G

V

A

R

K

G

K

A

E

A

V

L

I

K

N

E

Q

R

R

V

V

H

N

T

Q

M

V

L

A

R

E

A

V

F

L

R

Q

L

L

D

-

T

-

D

-

L

-

A

A

H

H

M

L

A

L

S

D

R

R

Y

K

P

P

A

K

Q

A

L

L

S

S

A

G

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

C

A

D

E

P

P

A

S

V

V

L

F

L

L

D

D

E

E

P

P

F

L

A

S

N

N

L

L

D

D

R

A

G

A

L

L

R

R

G

V

E

Q

M

M

A

R

A

I

F

E

V

I

R

S

D

R

V

L

V

H

R

K

R

R

F

L

G

G

V

R

A

T

T

M

V

I

T

Y

V

V

T

T

H

H

D

D

L

Q

E

V

E

E

A

A

F

M

A

T

I

L

G

A

D

D

R

K

L

I

G

V

V

V

M

L

L

D

G

A

G

G

T

R

L

V

A

A

Q

Q

L

V

A

G

P

K

P

P

L

L

D

E

V

L

Y

Y

R

H

H

Y

P

P

A

A

D

D

E

R

A

F

T

V

A

A

R

G

F

F

L

I

G

G

binding adenosine-5'-diphosphate: V10 (= V17), S30 (= S37), G31 (= G38), C32 (≠ V39), G33 (= G40), K34 (= K41), S35 (≠ T42), T36 (= T43)

1g291 Malk (see paper)
40% identity, 63% coverage: 18:240/354 of query aligns to 19:249/372 of 1g291

query
sites
1g291

L

V

H

R

D

E

V

M

N

S

L

L

T

E

A

V

A

K

A

D

G

G

E

E

V

F

V

M

C

I

L

L

V

L

G

G

P
|
P

S
|
S

G
|
G

V
x
C

G
|
G

K
|
K

T
|
T

L

T

L

L

R

R

C

M

I

I

A

A

G

G

L

L

D

E

A

E

P

P

D

S

E

R

G

G

T

Q

I

I

R

Y

V

I

-

G

-

D

-

K

-

L

-

V

-

A

-
x
D

-

P

-
x
E

-
x
K

-

G

-

I

-

F

T

V

P

P

Q
x
K

G
x
D

H

R

G

D

G

-

V

I

V

A

L

M

V

V

F

F

Q

Q

D

S

Y

Y

L

A

L

L

F

Y

P

P

H

H

L

M

S

T

V

V

F

Y

E

D

N

N

V

I

A

A

F

F

G

P

P

L

R

K

A

L

R

R

G

K

V

V

R

P

G

R

A

Q

A

E

L

I

K

D

E

Q

R

R

V

V

H

R

T

E

M

V

L

A

R

E

A

L

F

L

R

-

L

-

D

-

T

-

D

-

D

G

L

L

A

T

H

E

M

L

A

L

S

N

R

R

Y

K

P

P

A

R

Q

E

L

L

S

S

A

G

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

L

A

G

R

R

A

A

L

I

V

V

C

R

D

K

P

P

A

Q

V

V

L

F

L

L

L

M

D

D

E

E

P

P

F

L

A

S

N

N

L

L

D

D

R

A

G

K

L

L

R

R

G

V

E

R

M

M

A

R

A

A

F

E

V

L

R

K

D

K

V

L

V

Q

R

R

R

Q

F

L

G

G

V

V

A

T

T

T

V

I

T

Y

V

V

T

T

H

H

D

D

L

Q

E

V

E

E

A

A

F

M

A

T

I

M

G

G

D

D

R

R

L

I

G

A

V

V

M

M

L

N

G

R

G

G

T

V

L

L

A

Q

Q

Q

L

V

A

G

P

S

P

P

L

D

D

E

V

V

Y
|
Y

R
x
D

H

K

P

P

A

A

D

N

E

T

A

F

T

V

A

A

R

G

F

F

L

I

G

G

-

S

-

P

P

P

V

M

T

N

V

F

L

L

D

D

binding magnesium ion: D69 (vs. gap), E71 (vs. gap), K72 (vs. gap), K79 (≠ Q65), D80 (≠ G66), Y228 (= Y221), D229 (≠ R222)
binding pyrophosphate 2-: P37 (= P36), S38 (= S37), G39 (= G38), C40 (≠ V39), G41 (= G40), K42 (= K41), T43 (= T42), T44 (= T43)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 341, 342, 359

7cagC Mycobacterium smegmatis lpqy-sugabc complex in the catalytic intermediate state (see paper)
37% identity, 73% coverage: 18:276/354 of query aligns to 16:276/362 of 7cagC

query
sites
7cagC

L

V

H

K

D

E

V

F

N

S

L

M

T

T

A

I

A

A

A

D

G

G

E

E

V

F

V

I

C

I

L

L

V

V

G

G

P
|
P

S
|
S

G
|
G

V
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

T

L

L

R

N

C

M

I

I

A

A

G

G

L

L

D

E

A

E

P

I

D

T

E

S

G

G

T

E

I

L

R

R

-

I

-

G

-

E

-

R

V

V

T

N

P

E

P

K

Q

A

G

P

H

K

G

D

G

R

G

D

V

I

V

A

L

M

V

V

F

F

Q
|
Q

D

S

Y

Y

L

A

L

L

F

Y

P

P

H

H

L

M

S

T

V

V

F

R

E

Q

N

N

V

I

A

A

F

F

G

P

P

L

R

T

A

L

R

A

G

K

V

V

R

P

G

K

A

A

A

E

L

I

K

A

E

A

R

K

V

V

H

E

T

E

M

T

L

A

R

K

A

I

F

L

R

-

L

-

D

-

T

-

D

-

D

D

L

L

A

S

H

E

M
x
L

A

L

S

D

R
|
R

Y

K

P

P

A

G

Q
|
Q

L

L

S
|
S

A
x
G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

V

V

C

R

D

S

P

P

A

K

V

A

L

F

L

L

L

M

D

D

E

Q

P

P

F

L

A

S

N

N

L

L

D

D

R

A

G

K

L

L

R

R

G

V

E

Q

M

M

A

R

A

A

F

E

V

I

R

S

D

R

V

L

V

Q

R

D

R

R

F

L

G

G

V

T

A

T

T

T

V

V

T

Y

V

V

T

T

H

H

D

D

L

Q

E

T

E

E

A

A

F

M

A

T

I

L

G

G

D

D

R

R

L

V

G

V

V

V

M

M

L

L

G

A

G

G

T

E

L

V

A

Q

Q

Q

L

I

A

G

P

T

P

P

L

D

D

E

V

L

Y

Y

R

S

H

S

P

P

A

A

D

N

E

L

A

F

T

V

A

A

R

G

F

F

L

I

G

G

-

S

P

P

V

A

T

M

V

N

L

F

D

F

E

P

T

A

T

T

R

R

R

T

T

D

L

V

G

G

I

V

D

R

T

L

P

P

P

F

A

G

A

E

A

V

T

T

A

L

C

T

A

P

D

H

T

M

M

L

Q

D

G

L

L

L

-

D

R

K

L

Q

Y

A

R

R

P

P

E

E

A

N

L

I

A

I

V

V

binding adenosine-5'-triphosphate: P34 (= P36), S35 (= S37), G36 (= G38), C37 (≠ V39), G38 (= G40), K39 (= K41), S40 (≠ T42), T41 (= T43), Q79 (= Q76), L123 (≠ M125), R126 (= R128), Q130 (= Q132), S132 (= S134), G133 (≠ A135), G134 (= G136), Q135 (= Q137)
binding magnesium ion: