SitesBLAST

Q9FFF4 Acetolactate synthase small subunit 2, chloroplastic; ALS-interacting protein 3; Acetohydroxy-acid synthase small subunit 2; Protein valine-tolerant 1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
36% identity, 65% coverage: 14:83/107 of query aligns to 310:386/477 of Q9FFF4

query
sites
Q9FFF4

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Sites not aligning to the query:

88 G→D: Resistance to valine ihibition.
119 T→I: In vat1-1; resistance to valine ihibition.
453 R→Q: In vat1-3; resistance to valine ihibition.

6vz8G Arabidopsis thaliana acetohydroxyacid synthase complex with valine bound (see paper)
39% identity, 65% coverage: 14:83/107 of query aligns to 6:82/159 of 6vz8G

query
sites
6vz8G

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binding valine: D12 (≠ N20), G15 (= G23), V16 (= V24), L17 (≠ M25), N30 (= N38), I31 (≠ V39), V36 (= V47)

P9WKJ3 Putative acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS; EC 2.2.1.6 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
33% identity, 87% coverage: 14:106/107 of query aligns to 8:101/168 of P9WKJ3

query
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P9WKJ3

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K44 (≠ E49) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Q93YZ7 Acetolactate synthase small subunit 1, chloroplastic; ALS-interacting protein 1; Acetohydroxyacid synthase small subunit 1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
39% identity, 65% coverage: 14:83/107 of query aligns to 322:398/491 of Q93YZ7

query
sites
Q93YZ7

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D328 (≠ N20) binding
G331 (= G23) mutation to D: Decreased feedback inhibition by leucine and valine/isoleucine.
V332 (= V24) binding
L333 (≠ M25) binding
F340 (= F32) mutation to A: Decreased feedback inhibition by valine/isoleucine.
Y345 (≠ F37) mutation to A: No effect.
N346 (= N38) binding ; mutation N->A,H: Decreased feedback inhibition by leucine and increased feedback inhibition by valine/isoleucine.
I347 (≠ V39) binding

Sites not aligning to the query:

95 binding
98 G→E: Increased feedback inhibition by valine/isoleucine.
99 binding
100 binding
107 F→A: Decreased feedback inhibition by leucine and valine/isoleucine.
112 Y→A: Increased feedback inhibition by leucine.
113 binding ; N→A: Decreased feedback inhibition by valine/isoleucine.
114 binding

A0QUX7 Acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS; EC 2.2.1.6 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
32% identity, 71% coverage: 13:88/107 of query aligns to 9:86/170 of A0QUX7

query
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A0QUX7

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K46 (≠ G50) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

2pc6A Crystal structure of putative acetolactate synthase- small subunit from nitrosomonas europaea (see paper)
31% identity, 68% coverage: 14:86/107 of query aligns to 6:80/164 of 2pc6A

query
sites
2pc6A

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binding calcium ion: N66 (≠ R72), L68 (= L74), V71 (= V77)

O60086 Probable acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
34% identity, 62% coverage: 18:83/107 of query aligns to 77:144/289 of O60086

query
sites
O60086

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Sites not aligning to the query:

34 modified: Phosphoserine

Query Sequence

>209295 MicrobesOnline__882:209295
MNGAAPAAGGLIALRLTVNNHPGVMTHICGLFARRAFNVEGILCMPVGEGTSRIWLLVNE
DERLEQMIRQVRKLQDVLEVLPFPADMSAFTRLEDSMKVWESGGCRL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory