SitesBLAST

Comparing 209431 DVU0494 aminotransferase, class V to proteins with known functional sites using BLASTp with E ≤ 0.001.

Found 20 (the maximum) hits to proteins with known functional sites

2dr1A Crystal structure of the ph1308 protein from pyrococcus horikoshii ot3
32% identity, 88% coverage: 4:346/389 of query aligns to 16:361/381 of 2dr1A

• binding pyridoxal-5'-phosphate: S75 (= S62), G76 (= G63), T77 (≠ S64), F102 (= F89), R105 (= R92), T153 (= T140), D178 (= D165), V180 (≠ I167), S181 (= S168), S201 (≠ G188), Q203 (= Q190), K204 (= K191), S252 (≠ F238), T253 (= T239)

1iugA The crystal structure of aspartate aminotransferase which belongs to subgroup iv from thermus thermophilus (see paper)
34% identity, 86% coverage: 7:342/389 of query aligns to 3:329/348 of 1iugA

• binding phosphate ion: K84 (= K88), R88 (= R92), K184 (= K191)

3kgwB Crystal structure of putative aminotransferase (aah25799.1) from mus musculus at 1.65 a resolution
32% identity, 91% coverage: 7:360/389 of query aligns to 25:385/388 of 3kgwB

• binding pyridoxal-5'-phosphate: S81 (= S64), G82 (= G65), H83 (vs. gap), W108 (≠ F89), G156 (≠ S138), S158 (≠ T140), D183 (= D165), V185 (≠ I167), A186 (≠ S168), S206 (≠ G188), Q208 (= Q190), K209 (= K191)

3kgxA Crystal structure of putative aminotransferase (aah25799.1) from mus musculus at 1.80 a resolution
32% identity, 91% coverage: 7:360/389 of query aligns to 21:380/383 of 3kgxA

• binding magnesium ion: H290 (≠ L273), H294 (≠ G277), D367 (= D345)

5hhyA Structure of human alanine:glyoxylate aminotransferase major allele (agt-ma) showing x-ray induced reduction of plp internal aldimine to 4'-deoxy-piridoxine-phosphate (plr) (see paper)
33% identity, 86% coverage: 7:339/389 of query aligns to 20:360/385 of 5hhyA

• binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: S76 (= S64), G77 (= G65), H78 (vs. gap), W103 (≠ F89), S153 (≠ T140), D178 (= D165), V180 (≠ I167), A181 (≠ S168), Q203 (= Q190), K204 (= K191), Y255 (≠ F238), H257 (vs. gap), T258 (= T239)

P21549 Alanine--glyoxylate aminotransferase; AGT; Serine--pyruvate aminotransferase; SPT; EC 2.6.1.44; EC 2.6.1.51 from Homo sapiens (Human) (see 24 papers)
33% identity, 86% coverage: 7:339/389 of query aligns to 25:365/392 of P21549

• R36 (= R18) to C: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177157
• G41 (≠ M23) to E: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177168; to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of protein stability; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; partial mitochondrial mistargeting; intraperoxisomal protein aggregation seen; dbSNP:rs121908523; to V: in HP1; reduced alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177168
• G47 (≠ H29) to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity; reduced expression levels; reduced pyridoxal phosphate binding; reduced dimerization; reduced thermostability; increased propensity to aggregation; increased susceptibility to proteolytic degradation within the N-terminal region; mitochondrial mistargeting; exposure to pyridoxine can rescue the functionality by partially preventing aggregation and degradation and by redirecting all the protein to the peroxisome; dbSNP:rs180177173
• G82 (= G65) to E: in HP1; abolishes alanine--glyoxylate aminotransferase activity by interfering with pyridoxal phosphate binding; dbSNP:rs121908522
• W108 (≠ F89) to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs180177197
• A112 (≠ W93) to D: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs796052061
• L150 (= L134) to P: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177222
• F152 (≠ Q136) to I: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in protein destabilization; decreased alanine--glyoxylate aminotransferase activity; no loss of dimerization; mitochondrial mistargeting; dbSNP:rs121908524
• G156 (vs. gap) to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs121908530
• S158 (≠ T140) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177225
• G161 (= G143) to C: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227; to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; loss of dimerization; dbSNP:rs180177227; to S: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227
• L166 (≠ V148) to P: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177230
• G170 (≠ A152) to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in mitochondrial mistargeting; slight decrease in alanine--glyoxylate aminotransferase activity; loss of dimerization; partial loss of protein stability but protein stability increases in the presence of pyridoxal phosphate; causes protein aggregation; dbSNP:rs121908529
• C173 (≠ T155) to Y: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs180177231
• D183 (= D165) to N: in HP1; loss of alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177236
• S187 (≠ A169) to F: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization but improved dimerization in the presence of pyridoxal phosphate; decreased protein stability; dbSNP:rs180177238
• I202 (≠ C184) to N: in HP1; unknown pathological significance; dbSNP:rs536352238
• S205 (≠ T187) to P: in HP1; loss of alanine--glyoxylate aminotransferase activity; decreased protein stability; dbSNP:rs121908520
• K209 (= K191) mutation to R: Affects pyridoxal phosphate binding; loss of alanine--glyoxylate aminotransferase activity.
• S218 (≠ A200) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; no effect on protein stability; dbSNP:rs180177253
• R233 (≠ D213) to C: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908526; to H: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908527
• I244 (≠ L224) to T: in HP1; prevalent mutation in the Canary islands; when associated with L-11 and M-340 on the minor AGXT allele; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity; no loss of dimerization; partial mitochondrial mistargeting; dbSNP:rs121908525
• C253 (= C231) to R: in HP1; when associated with L-11 and M-340 on the minor AGXT allele; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177264
• I279 (≠ F255) to T: in dbSNP:rs140992177
• A280 (≠ L256) to V: in dbSNP:rs73106685
• V326 (≠ L300) to I: in dbSNP:rs115057148
• I340 (≠ A314) to M: associated with hyperoxaluria; dbSNP:rs4426527

Sites not aligning to the query:

• 9 T → N: no loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs115014558
• 11 P → L: reduction of specific alanine--glyoxylate aminotransferase activity in vitro; causes mitochondrial mistargeting when associated with R-170; dbSNP:rs34116584

6rv0A Human alanine:glyoxylate aminotransferase major allele (agt-ma); with pmp in the active site (see paper)
33% identity, 86% coverage: 7:339/389 of query aligns to 20:360/384 of 6rv0A

• binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S76 (= S64), G77 (= G65), H78 (vs. gap), W103 (≠ F89), S153 (≠ T140), D178 (= D165), V180 (≠ I167), A181 (≠ S168), Q203 (= Q190), K204 (= K191)

1vjoA Crystal structure of alanine--glyoxylate aminotransferase (alr1004) from nostoc sp. At 1.70 a resolution (see paper)
32% identity, 86% coverage: 7:340/389 of query aligns to 21:358/377 of 1vjoA

• binding pyridoxal-5'-phosphate: T77 (≠ S64), G78 (= G65), T79 (vs. gap), F104 (= F89), T154 (= T140), D179 (= D165), V181 (≠ I167), T182 (≠ S168), Q204 (= Q190), K205 (= K191)

1j04A Structural mechanism of enzyme mistargeting in hereditary kidney stone disease in vitro (see paper)
33% identity, 86% coverage: 7:339/389 of query aligns to 22:362/387 of 1j04A

• binding (aminooxy)acetic acid: P25 (= P10), G26 (= G11), M345 (≠ A322), L348 (≠ Q325), R357 (= R334)

6pk1A Alanine-glyoxylate aminotransferase 1 (agt1) from arabidopsis thaliana in presence of serine (see paper)
28% identity, 97% coverage: 4:382/389 of query aligns to 7:389/399 of 6pk1A

• binding 3-hydroxypyruvic acid: P13 (= P10), G14 (= G11), F93 (= F89), T146 (= T140), K199 (= K191), R345 (= R334)
• binding pyridoxal-5'-phosphate: T66 (≠ S62), G67 (= G63), T68 (≠ S64), W71 (≠ M67), F93 (= F89), T146 (= T140), D173 (= D165), V175 (≠ I167), Q198 (= Q190), K199 (= K191)

6pk3B Alanine-glyoxylate aminotransferase 1 (agt1) from arabidopsis thaliana (see paper)
28% identity, 97% coverage: 4:382/389 of query aligns to 8:390/400 of 6pk3B

• binding pyridoxal-5'-phosphate: T67 (≠ S62), G68 (= G63), T69 (≠ S64), W72 (≠ M67), F94 (= F89), T147 (= T140), D174 (= D165), V176 (≠ I167), Q199 (= Q190), K200 (= K191)

Q56YA5 Serine--glyoxylate aminotransferase; Alanine--glyoxylate aminotransferase; AGT; Asparagine aminotransferase; Serine--pyruvate aminotransferase; EC 2.6.1.45; EC 2.6.1.44; EC 2.6.1.-; EC 2.6.1.51 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
28% identity, 97% coverage: 4:382/389 of query aligns to 9:391/401 of Q56YA5

• TGT 68:70 (≠ SGS 62:64) binding
• T148 (= T140) binding
• QK 200:201 (= QK 190:191) binding
• K201 (= K191) binding
• P251 (= P241) mutation to L: Abolishes aminotransferase activity.
• R347 (= R334) binding

1h0cA The crystal structure of human alanine:glyoxylate aminotransferase (see paper)
33% identity, 86% coverage: 7:339/389 of query aligns to 22:360/385 of 1h0cA