SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 209435 MicrobesOnline__882:209435 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

7bkcK Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from methanospirillum hungatei (dimeric, composite structure) (see paper)
37% identity, 21% coverage: 319:424/501 of query aligns to 13:95/321 of 7bkcK

query
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7bkcK

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binding iron/sulfur cluster: C36 (= C343), T37 (= T344), C39 (= C346), C42 (= C349), C46 (= C353), P47 (= P354), I51 (≠ L358), C77 (= C406), S78 (≠ L407), Y79 (≠ G408), C80 (= C409), G81 (= G410), C83 (= C412), C87 (= C416), L92 (= L421)

Sites not aligning to the query:

binding iron/sulfur cluster: 119, 124, 125, 127, 130, 134, 135, 139, 178, 183, 184, 185, 186, 187, 189, 193, 194, 198, 208, 215, 216, 219, 221, 222, 226, 257, 258, 259, 260, 261, 263, 267, 268

7bkbK Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from methanospirillum hungatei (hexameric, composite structure) (see paper)
37% identity, 21% coverage: 319:424/501 of query aligns to 13:95/386 of 7bkbK

query
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7bkbK

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binding iron/sulfur cluster: C36 (= C343), T37 (= T344), C39 (= C346), C42 (= C349), C46 (= C353), P47 (= P354), I51 (≠ L358), C77 (= C406), S78 (≠ L407), Y79 (≠ G408), C80 (= C409), G81 (= G410), C83 (= C412), C87 (= C416), L92 (= L421)

Sites not aligning to the query:

binding iron/sulfur cluster: 119, 124, 125, 127, 130, 134, 139, 173, 174, 175, 176, 179, 183, 186, 211, 212, 214, 215, 217, 221, 222, 241, 248, 249, 251, 254, 258, 259, 263, 265, 273, 280, 281, 284, 286, 287, 291, 322, 323, 324, 325, 326, 328, 332, 333

7bkbL Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from methanospirillum hungatei (hexameric, composite structure) (see paper)
30% identity, 18% coverage: 339:427/501 of query aligns to 7:78/79 of 7bkbL

query
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7bkbL

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binding iron/sulfur cluster: C11 (= C343), T12 (= T344), C14 (= C346), N15 (≠ G347), C17 (= C349), C21 (= C353), P22 (= P354), Y40 (≠ G378), A47 (= A385), C57 (= C406), A58 (≠ L407), G59 (= G408), C60 (= C409), G61 (= G410), C63 (= C412), C67 (= C416), Y69 (≠ T418), I72 (≠ L421)

8e9iI Mycobacterial respiratory complex i, semi-inserted quinone (see paper)
36% identity, 17% coverage: 341:423/501 of query aligns to 50:114/166 of 8e9iI

query
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8e9iI

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binding iron/sulfur cluster: C52 (= C343), I53 (≠ T344), G54 (= G345), C55 (= C346), E56 (≠ G347), L57 (= L348), C58 (= C349), C62 (= C353), I67 (≠ L358), Y90 (≠ L399), C97 (= C406), I98 (≠ L407), G99 (= G408), C100 (= C409), G101 (= G410), C103 (= C412), C107 (= C416), P108 (= P417)

Sites not aligning to the query:

binding iron/sulfur cluster: 40

Query Sequence

>209435 MicrobesOnline__882:209435
MMTARTDAARGTSRPATLAVNVHTTYTTRQHDTWGRHTLSPARHTTGRHMGHLTGKDVYR
RLAGKLDNTPVRTPDTPAFRALLRELYTPEEADLVACMPYRPATASRIAAVCRMGRARVE
AMLDTLCAKGLVIDLREGDTATYMISPIVIGIFEFTMMRTGGDLPHGTWARLFNDYMFGD
TTFFDANFASGQRISIMRALPHEEAVEEAARTEILDHERARALVDRHTTFAVGLCSCRHE
KVHIGEEPCDAPLETCTSLGSGAEFLIRNGFARRIDRAEMLDILARSRDLGLTLSTDNVR
RDAGFICHCCGCCCNLMRGIKVTGHTGILVTSNWLATCDADACTGCGLCVRACPVDALHV
VRKDGQRTASNETVDARGDVPDDEAASSTARRGKARRRLEVDTSVCLGCGVCALRCPTGA
LRLEERPARVFHPEDSFERVILQSLERGTFQNLIFDNPASRSQAFMRGLVGGFLRLDPVK
RALMGDRLRSRFLGAIRRVAT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory