SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 209495 MicrobesOnline__882:209495 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4zrnA Crystal structure of udp-glucose 4-epimerase (tm0509) with udp-glucose from hyperthermophilic eubacterium thermotoga maritima (see paper)
35% identity, 97% coverage: 7:308/312 of query aligns to 4:304/309 of 4zrnA

query
sites
4zrnA

C

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active site: T117 (≠ S121), G119 (= G123), A120 (= A124), Y143 (= Y146), K147 (= K150), Y181 (≠ S184), G185 (= G188)
binding nicotinamide-adenine-dinucleotide: G7 (= G10), G10 (= G13), F11 (= F14), I12 (≠ L15), D31 (≠ S34), N32 (≠ R35), S34 (= S37), S35 (≠ R38), G36 (≠ R39), S51 (≠ D57), I52 (≠ F58), L73 (= L77), A74 (≠ V78), A75 (≠ S79), T92 (≠ E96), S115 (≠ A119), S116 (= S120), Y143 (= Y146), K147 (= K150), Y170 (≠ I173), V173 (≠ P176)
binding uridine-5'-diphosphate-glucose: T117 (≠ S121), G119 (= G123), A120 (= A124), Y143 (= Y146), N172 (= N175), G185 (= G188), V186 (= V189), H201 (= H204), F203 (≠ W206), Y208 (≠ V211), R210 (= R213), V244 (≠ L248), R267 (= R271), D270 (= D274)

2p5uA Crystal structure of thermus thermophilus hb8 udp-glucose 4-epimerase complex with NAD
34% identity, 96% coverage: 4:303/312 of query aligns to 1:302/311 of 2p5uA

query
sites
2p5uA

F

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active site: T117 (≠ S121), G119 (= G123), A120 (= A124), Y143 (= Y146), K147 (= K150), H181 (≠ S184), G185 (= G188)
binding nicotinamide-adenine-dinucleotide: G10 (= G13), F11 (= F14), I12 (≠ L15), D31 (vs. gap), N32 (vs. gap), L33 (vs. gap), A34 (≠ S34), T35 (≠ R35), G36 (≠ D36), D51 (= D57), L52 (≠ F58), Q73 (≠ L77), A74 (≠ V78), A75 (≠ S79), A77 (≠ T81), S116 (= S120), Y143 (= Y146), K147 (= K150), V173 (≠ P176)

6dntA Udp-n-acetylglucosamine 4-epimerase from methanobrevibacter ruminantium m1 in complex with udp-n-acetylmuramic acid (see paper)
32% identity, 91% coverage: 1:283/312 of query aligns to 1:281/310 of 6dntA

query
sites
6dntA

M

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active site: S120 (= S121), S121 (≠ G122), A122 (= A124), Y144 (= Y146), K148 (= K150), A187 (≠ G188)
binding (2R)-2-{[(2R,3R,4R,5S,6R)-3-(acetylamino)-2-{[(S)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-5-hydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-4-yl]oxy}propanoic acid: A80 (≠ T81), S120 (= S121), S121 (≠ G122), Y144 (= Y146), F172 (≠ S174), N173 (= N175), A187 (≠ G188), V188 (= V189), K191 (≠ V192), V203 (≠ H204), I204 (≠ V205), Y205 (≠ W206), Q210 (≠ V211), R212 (= R213), I246 (≠ L248), R269 (= R271), D272 (= D274)
binding nicotinamide-adenine-dinucleotide: G10 (= G10), G13 (= G13), F14 (= F14), I15 (≠ L15), D33 (≠ F33), N34 (≠ S34), L35 (≠ R35), S36 (≠ D36), S37 (= S37), G38 (≠ R38), D57 (= D57), L58 (≠ F58), L76 (= L77), A77 (≠ V78), A78 (≠ S79), A80 (≠ T81), S118 (≠ A119), S119 (= S120), Y144 (= Y146), K148 (= K150), Y171 (≠ I173), V174 (≠ P176)
binding zinc ion: E209 (≠ S210), H275 (≠ Y277)

7ysmA Crystal structure of udp-glucose 4-epimerase (rv3634c) co-crystallized with udp-n-acetylglucosamine from mycobacterium tuberculosis
36% identity, 96% coverage: 4:301/312 of query aligns to 1:301/311 of 7ysmA

query
sites
7ysmA

F

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Y

Y

L

L

A

N

L

T

Y

F

H

R

E

H

L

L

Y

Y

G

G

I

L

D

D

Y

C

A

S

A

H

L

I

R

A

I
x
P

S
x
A

N
|
N

P
x
V

F

Y

G

G

P

P

L

R

Q

Q

R

D

T

P

S

H

A

G

E

E

Q

A

G
|
G

V
|
V

I

V

G

A

V

I

F
|
F

L

A

G

Q

K

A

I

L

L

L

R

S

N

G

E

K

P

P

L

T

H
x
R

V
|
V

W
x
F

G

G

D

D

G

G

S

T

V
x
N

V

T

R
|
R

D

D

Y

Y

I

V

Y

F

V

V

E

D

D

D

V

V

A

V

R

D

A

A

L

F

V

V

L

R

A

V

A

S

R

A

L

D

N

V

T

G

E

G

H

G

H

L

V

R

F

F

N

N

I

I

G

G

S

T

G

G

A

K

G

E

L

T

S

S

L
x
D

N

R

D

Q

I

L

I

H

D

S

M

A

M

V

R

A

S

A

V

A

T

V

G

G

R

G

D

P

V

D

V

D

V

P

K

E

Y

F

D

H

Q

P

N

P

R
|
R

V

L

F

G

D

D

V

L

P

K

Y

R

S

S

V

C

L

L

D

D

V

I

S

G

L

L

A

A

E

E

R

R

E

V

L

L

G

G

W

W

R

R

A

P

L

Q

F

I

P

E

F

L

K

A

V

D

G

G

V

V

binding nicotinamide-adenine-dinucleotide: G7 (= G10), G10 (= G13), F11 (= F14), I12 (≠ L15), D31 (≠ S34), N32 (≠ R35), T35 (≠ S37), G36 (≠ R38), D56 (≠ F58), I57 (≠ F59), L77 (= L77), A78 (≠ V78), A79 (≠ S79), I81 (≠ T81), T119 (≠ A119), Y146 (= Y146), K150 (= K150), P173 (≠ I173), N175 (= N175), V176 (≠ P176)
binding uridine-diphosphate-n-acetylgalactosamine: I81 (≠ T81), R84 (≠ K84), S121 (= S121), G123 (= G123), S124 (≠ A124), Y146 (= Y146), A174 (≠ S174), N175 (= N175), G188 (= G188), V189 (= V189), F193 (= F193), R204 (≠ H204), V205 (= V205), F206 (≠ W206), N211 (≠ V211), R213 (= R213), D248 (≠ L248), R271 (= R271)

7ys9A Crystal structure of udp-glucose 4-epimerase (rv3634c) in complex with both udp-glucose and udp-galactose in chaina from mycobacterium tuberculosis
36% identity, 96% coverage: 4:301/312 of query aligns to 1:301/310 of 7ys9A

query
sites
7ys9A

F

M

R

R

A

A

C

L

V

V

L

T

G
|
G

A

A

S

A

G
|
G

F
|
F

L
x
I

G

G

S

S

H

T

L

L

V

V

H

D

H

R

L

L

L

L

K

A

A

D

G

G

C

H

Q

S

V

V

H

V

A

G

F

L

S
x
D

R
x
N

-

F

D
x
A

S
x
T

R
x
G

R

R

N

A

P

T

L

N

L

L

T

E

D

H

E

L

L

A

M

D

S

N

S

S

C

A

S

H

I

V

F

F

T

V

G

E

D

A

F
x
D

F
x
I

N

V

T

T

Q

A

D

D

V

L

E

H

R

A

A

I

L

L

A

E

D

Q

-

H

-

R

C

P

D

E

V

V

C

V

F

F

H

H

L
|
L

V
x
A

S
x
A

T

Q

T
x
I

I

D

P

V

K
x
R

T

R

S

S

N

V

D

A

D

D

P

P

L

Q

R

F

D

D

V

A

R

A

E

V

N

N

L

V

S

I

G

G

S

T

L

V

T

R

L

L

L

A

E

E

C

A

V

A

R

R

R

Q

T

T

G

G

V

V

R

R

K

K

V

I

V

V

Y

H

A
x
T

S

S

S
|
S

G

G

G
|
G

A

S

I

I

Y

Y

G

G

K

T

H

P

L

P

M

E

P

Y

R

P

I

T

S

P

E

E

N

T

H

A

P

P

T

T

D

D

P

P

L

A

C

S

S

P

Y
|
Y

G

A

I

A

V

G

K
|
K

L

V

A

A

V

G

E

E

K

I

Y

Y

L

L

A

N

L

T

Y

F

H

R

E

H

L

L

Y

Y

G

G

I

L

D

D

Y

C

A

S

A

H

L

I

R

A

I
x
P

S
x
A

N
|
N

P
x
V

F

Y

G

G

P

P

L

R

Q

Q

R

D

T

P

S

H

A

G

E

E

Q
x
A

G
|
G

V
|
V

I

V

G

A

V

I

F
|
F

L

A

G

Q

K

A

I

L

L

L

R

S

N

G

E

K

P

P

L

T

H
x
R

V

V

W
x
F

G

G

D

D

G

G

S

T

V
x
N

V

T

R
|
R

D

D

Y

Y

I

V

Y

F

V

V

E

D

D

D

V

V

A

V

R

D

A

A

L

F

V

V

L

R

A

V

A

S

R

A

L

D

N

V

T

G

E

G

H

G

H

L

V

R

F

F

N

N

I

I

G

G

S

T

G

G

A

K

G

E

L

T

S

S

L
x
D

N

R

D

Q

I

L

I

H

D

S

M

A

M

V

R

A

S

A

V

A

T

V

G

G

R

G

D

P

V

D

V

D

V

P

K

E

Y

F

D

H

Q

P

N

P

R
|
R

V

L

F

G

D

D

V

L

P

K

Y

R

S

S

V

C

L

L

D

D

V

I

S

G

L

L

A

A

E

E

R

R

E

V

L

L

G

G

W

W

R

R

A

P

L

Q

F

I

P

E

F

L

K

A

V

D

G

G

V

V

binding galactose-uridine-5'-diphosphate: I81 (≠ T81), R84 (≠ K84), S121 (= S121), G123 (= G123), Y146 (= Y146), A174 (≠ S174), N175 (= N175), A187 (≠ Q187), G188 (= G188), V189 (= V189), F193 (= F193), R204 (≠ H204), F206 (≠ W206), N211 (≠ V211), R213 (= R213), D248 (≠ L248), R271 (= R271)
binding nicotinamide-adenine-dinucleotide: G7 (= G10), G10 (= G13), F11 (= F14), I12 (≠ L15), D31 (≠ S34), N32 (≠ R35), A34 (≠ D36), T35 (≠ S37), G36 (≠ R38), D56 (≠ F58), I57 (≠ F59), L77 (= L77), A78 (≠ V78), A79 (≠ S79), I81 (≠ T81), T119 (≠ A119), Y146 (= Y146), K150 (= K150), P173 (≠ I173), A174 (≠ S174), V176 (≠ P176)
binding uridine-5'-diphosphate-glucose: I81 (≠ T81), R84 (≠ K84), S121 (= S121), G123 (= G123), Y146 (= Y146), A174 (≠ S174), N175 (= N175), A187 (≠ Q187), G188 (= G188), V189 (= V189), F193 (= F193), R204 (≠ H204), F206 (≠ W206), N211 (≠ V211), R213 (= R213), D248 (≠ L248), R271 (= R271)

7ystA Crystal structure of udp-glucose 4-epimerase (rv3634c) in complex with both udp-glucose and udp-galactose in chain b from mycobacterium tuberculosis
36% identity, 96% coverage: 4:301/312 of query aligns to 1:301/312 of 7ystA

query
sites
7ystA

F

M

R

R

A

A

C

L

V

V

L

T

G
|
G

A

A

S

A

G
|
G

F
|
F

L
x
I

G

G

S

S

H

T

L

L

V

V

H

D

H

R

L

L

L

L

K

A

A

D

G

G

C

H

Q

S

V

V

H

V

A

G

F

L

S
x
D

R
x
N

-

F

D

A

S
x
T

R
x
G

R

R

N

A

P

T

L

N

L

L

T

E

D

H

E

L

L

A

M

D

S

N

S

S

C

A

S

H

I

V

F

F

T

V

G

E

D

A

F
x
D

F
x
I

N

V

T

T

Q

A

D

D

V

L

E

H

R

A

A

I

L

L

A

E

D

Q

-

H

-

R

C

P

D

E

V

V

C

V

F

F

H

H

L
|
L

V
x
A

S
x
A

T

Q

T
x
I

I

D

P

V

K
x
R

T

R

S

S

N

V

D

A

D

D

P

P

L

Q

R

F

D

D

V

A

R

A

E
x
V

N

N

L

V

S

I

G

G

S

T

L

V

T

R

L

L

L

A

E

E

C

A

V

A

R

R

R

Q

T

T

G

G

V

V

R

R

K

K

V

I

V

V

Y

H

A
x
T

S

S

S
|
S

G

G

G
|
G

A

S

I

I

Y

Y

G

G

K

T

H

P

L

P

M

E

P

Y

R

P

I

T

S

P

E

E

N

T

H

A

P

P

T

T

D

D

P

P

L

A

C

S

S

P

Y
|
Y

G

A

I

A

V

G

K
|
K

L

V

A

A

V

G

E

E

K

I

Y

Y

L

L

A

N

L

T

Y

F

H

R

E

H

L

L

Y

Y

G

G

I

L

D

D

Y

C

A

S

A

H

L

I

R

A

I
x
P

S
x
A

N
|
N

P
x
V

F

Y

G

G

P

P

L

R

Q

Q

R

D

T

P

S

H

A

G

E

E

Q
x
A

G
|
G

V
|
V

I

V

G

A

V

I

F
|
F

L

A

G

Q

K

A

I

L

L

L

R

S

N

G

E

K

P

P

L

T

H
x
R

V
|
V

W
x
F

G

G

D

D

G

G

S

T

V

N

V

T

R
|
R

D

D

Y

Y

I

V

Y

F

V

V

E

D

D

D

V

V

A

V

R

D

A

A

L

F

V

V

L

R

A

V

A

S

R

A

L

D

N

V

T

G

E

G

H

G

H

L

V

R

F

F

N

N

I

I

G

G

S

T

G

G

A

K

G

E

L

T

S

S

L
x
D

N

R

D

Q

I

L

I

H

D

S

M

A

M

V

R

A

S

A

V

A

T

V

G

G

R

G

D

P

V

D

V

D

V

P

K

E

Y

F

D

H

Q

P

N

P

R
|
R

V

L

F

G

D

D

V

L

P

K

Y

R

S

S

V

C

L

L

D

D

V

I

S

G

L

L

A

A

E

E

R

R

E

V

L

L

G

G

W

W

R

R

A

P

L

Q

F

I

P

E

F

L

K

A

V

D

G

G

V

V

binding nicotinamide-adenine-dinucleotide: G7 (= G10), G10 (= G13), F11 (= F14), I12 (≠ L15), D31 (≠ S34), N32 (≠ R35), T35 (≠ S37), G36 (≠ R38), D56 (≠ F58), I57 (≠ F59), L77 (= L77), A78 (≠ V78), A79 (≠ S79), I81 (≠ T81), V96 (≠ E96), T119 (≠ A119), Y146 (= Y146), K150 (= K150), P173 (≠ I173), A174 (≠ S174), N175 (= N175), V176 (≠ P176)
binding uridine-5'-diphosphate-glucose: I81 (≠ T81), R84 (≠ K84), S121 (= S121), G123 (= G123), Y146 (= Y146), A174 (≠ S174), N175 (= N175), A187 (≠ Q187), G188 (= G188), V189 (= V189), F193 (= F193), R204 (≠ H204), V205 (= V205), F206 (≠ W206), R213 (= R213), D248 (≠ L248), R271 (= R271)

6wj9B Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-glcnac (see paper)
31% identity, 89% coverage: 5:281/312 of query aligns to 3:281/308 of 6wj9B

query
sites
6wj9B

R

R

A

I

C

L

V

V

L

T

G
|
G

A

G

S

A

G
|
G

F
|
F

L
x
I

G

G

S

S

H

H

L

L

V

V

H

D

H

A

L

L

L

L

K

A

A

K

G

G

C

Y

Q

A

V

V

H

R

A

V

F

L

-
x
D

-
x
D

-

L

S
|
S

R
x
T

D
x
G

S

K

R

V

R

G

N

N

P

L

L

P

L

M

T

G

D

D

E

-

L

-

M

-

S

A

S

G

C

L

S

E

I

L

F

L

T

V

G

G

D
|
D

F
x
A

F

A

N

D

T

A

Q

A

D

L

V

L

E

A

R

D

A

A

L

V

A

Q

D

G

C

C

D

D

V

A

C

V

F

V

H

H

L
|
L

V
x
A

S
x
A

T

V

T

A

I

S

P
x
V

K

Q

T

A

S

S

N

V

D

E

D

D

P

P

L

V

R

A

D

T

V

H

R

Q

E
x
S

N

N

L

F

S

I

G

A

S

T

L

L

T

R

L

L

L

C

E

E

C

A

V

M

R

T

R

A

T

A

G

G

V

I

R

R

K

R

V

V

V

V

Y

F

A
|
A

S

S

S
x
A

G

-

G
x
A

A
|
A

I

V

Y

Y

G

G

K

N

H

N

-

G

L

E

M

G

P

T

R

P

I

I

S

A

E

E

N

D

H

T

P

P

T

K

D

S

P

P

L

L

C

T

S

P

Y
x
F

G

A

I

A

V

D

K
|
K

L

L

A

A

V

S

E

E

K

Y

Y

Y

L

L

A

D

L

F

Y

Y

H

R

E

R

L

Q

Y

H

G

G

I

L

D

E

Y

P

A

V

A

I

L

L

R

R

I
x
F

S
x
F

N
|
N

P
x
I

F

F

G

G

P

P

L

R

Q

Q

R

D

T

P

S

S

A

S

E

P

-

Y

Q

S

G
|
G

V
|
V

I

I

G

S

V

I

F
|
F

L

S

G

E

K

R

I

A

L

K

R

A

N

G

E

R

P

P

L

I

H
x
T

V
x
L

W
x
F

G

G

D

D

G

G

S

G

V

Q

V

T

R
|
R

D

D

Y

F

I

V

Y

Y

V

V

E

A

D

D

V

L

A

V

R

K

A

I

L

L

V

V

L

Q

A

G

A

L

R

E

L

S

N

P

T

A

E

P

-

A

H

A

H

D

V

A

F

T

N

N

I

V

G

G

S

L

G

G

A

G

G

V

L

T

S

T

L
|
L

N

N

D

D

I

L

I

I

D

G

M

A

M

L

R

Q

S

Q

V

I

T

S

G

G

R

K

D

P

V

L

V

Q

V

V

K

S

Y

H

D

G

Q

A

N

T

R
|
R

V

S

F

G

D
|
D

V

I

P

R

Y

H

S

S

V

K

L

A

D

D

active site: A119 (≠ S121), A120 (≠ G123), A121 (= A124), F144 (≠ Y146), K148 (= K150)
binding nicotinamide-adenine-dinucleotide: G8 (= G10), G11 (= G13), F12 (= F14), I13 (≠ L15), D32 (vs. gap), D33 (vs. gap), S35 (= S34), T36 (≠ R35), G37 (≠ D36), D55 (= D57), A56 (≠ F58), L75 (= L77), A76 (≠ V78), A77 (≠ S79), S94 (≠ E96), A117 (= A119), A119 (≠ S121), F144 (≠ Y146), K148 (= K150), F171 (≠ I173), F172 (≠ S174), I174 (≠ P176)
binding uridine-diphosphate-n-acetylglucosamine: V81 (≠ P83), N173 (= N175), G187 (= G188), V188 (= V189), F192 (= F193), T203 (≠ H204), L204 (≠ V205), F205 (≠ W206), R212 (= R213), L248 (= L248), R271 (= R271), D274 (= D274)

6wjaA Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-galnac (see paper)
31% identity, 89% coverage: 5:281/312 of query aligns to 2:280/307 of 6wjaA

query
sites
6wjaA

R

R

A

I

C

L

V

V

L

T

G
|
G

A

G

S

A

G
|
G

F
|
F

L
x
I

G

G

S

S

H

H

L

L

V

V

H

D

H

A

L

L

L

L

K

A

A

K

G

G

C

Y

Q

A

V

V

H

R

A

V

F

L

-
x
D

-
x
D

-

L

S
|
S

R
x
T

D
x
G

S

K

R

V

R

G

N

N

P

L

L

P

L

M

T

G

D

D

E

-

L

-

M

-

S

A

S

G

C

L

S

E

I

L

F

L

T

V

G

G

D

D

F
x
A

F

A

N

D

T

A

Q

A

D

L

V

L

E

A

R

D

A

A

L

V

A

Q

D

G

C

C

D

D

V

A

C

V

F

V

H

H

L
|
L

V
x
A

S
x
A

T

V

T

A

I

S

P
x
V

K

Q

T

A

S

S

N

V

D

E

D

D

P

P

L

V

R

A

D

T

V

H

R

Q

E
x
S

N

N

L

F

S

I

G

A

S

T

L

L

T

R

L

L

L

C

E

E

C

A

V

M

R

T

R

A

T

A

G

G

V

I

R

R

K

R

V

V

V

V

Y

F

A

A

S

S

S
x
A

G

-

G
x
A

A
|
A

I

V

Y

Y

G

G

K

N

H

N

-

G

L

E

M

G

P

T

R

P

I

I

S

A

E

E

N

D

H

T

P

P

T

K

D

S

P

P

L

L

C

T

S

P

Y
x
F

G

A

I

A

V

D

K
|
K

L

L

A

A

V

S

E

E

K

Y

Y

Y

L

L

A

D

L

F

Y

Y

H

R

E

R

L

Q

Y

H

G

G

I

L

D

E

Y

P

A

V

A

I

L

L

R

R

I
x
F

S
x
F

N
|
N

P
x
I

F

F

G

G

P

P

L

R

Q

Q

R

D

T

P

S

S

A

S

E

P

-

Y

Q

S

G
|
G

V
|
V

I

I

G

S

V

I

F
|
F

L

S

G

E

K

R

I

A

L

K

R

A

N

G

E

R

P

P

L

I

H
x
T

V

L

W
x
F

G

G

D

D

G

G

S

G

V

Q

V

T

R
|
R

D

D

Y

F

I

V

Y

Y

V

V

E

A

D

D

V

L

A

V

R

K

A

I

L

L

V

V

L

Q

A

G

A

L

R

E

L

S

N

P

T

A

E

P

-

A

H

A

H

D

V

A

F

T

N

N

I

V

G

G

S

L

G

G

A

G

G

V

L

T

S

T

L
|
L

N

N

D

D

I

L

I

I

D

G

M

A

M

L

R

Q

S

Q

V

I

T

S

G

G

R

K

D

P

V

L

V

Q

V

V

K

S

Y

H

D

G

Q

A

N

T

R
|
R

V

S

F

G

D
|
D

V

I

P

R

Y

H

S

S

V

K

L

A

D

D

active site: A118 (≠ S121), A119 (≠ G123), A120 (= A124), F143 (≠ Y146), K147 (= K150)
binding nicotinamide-adenine-dinucleotide: G7 (= G10), G10 (= G13), F11 (= F14), I12 (≠ L15), D31 (vs. gap), D32 (vs. gap), S34 (= S34), T35 (≠ R35), G36 (≠ D36), A55 (≠ F58), L74 (= L77), A75 (≠ V78), A76 (≠ S79), S93 (≠ E96), F143 (≠ Y146), K147 (= K150), F170 (≠ I173), F171 (≠ S174), I173 (≠ P176)
binding uridine-diphosphate-n-acetylgalactosamine: V80 (≠ P83), A120 (= A124), N172 (= N175), G186 (= G188), V187 (= V189), F191 (= F193), T202 (≠ H204), F204 (≠ W206), R211 (= R213), L247 (= L248), R270 (= R271), D273 (= D274)

1sb9A Crystal structure of pseudomonas aeruginosa udp-n-acetylglucosamine 4- epimerase complexed with udp-glucose (see paper)
30% identity, 96% coverage: 8:307/312 of query aligns to 20:334/340 of 1sb9A

query
sites
1sb9A

V

I

L

T

G
|
G

A

V

S

A

G
|
G

F
|
F

L
x
I

G

G

S

S

H

N

L

L

V

L

H

E

H

T

L

L

L

L

K

K

A

L

G

D

C

Q

Q

K

V

V

-

V

-

G

-

L

H
x
D

A
x
N

F
|
F

S

A

R
x
T

D
x
G

S

H

R

Q

R

R

N

N

-

L

-

D

-

E

-

V

-

R

P

S

L

L

L

V

T

S

D

E

E

K

L

Q

M

W

S

S

S

N

C

F

S

K

I

F

F

I

T

Q

G

G

D
|
D

F
x
I

F

R

N

N

T

L

Q

D

D

D

V

C

E

N

R

N

A

A

L

C

A

A

D

G

C

V

D

D

V

Y

C

V

F

L

H

H

L
x
Q

V

A

S
x
A

T

L

-

G

T

S

I

V

P

P

K

R

T

S

S

I

N

N

D

D

D

-

P

P

L

I

R

T

D

S

V

N

R

A

E
x
T

N

N

L

I

S

D

G

G

S

F

L

L

T

N

L

M

L

L

E

I

C

A

V

A

R

R

R

D

T

A

G

K

V

V

R

Q

K

S

V

F

V

T

Y

Y

A
|
A

S
x
A

S
|
S

G

-

G
x
S

A
x
S

I

T

Y

Y

G

G

K

D

H

H

L

P

-

G

M

L

P

P

R

K

I

V

S

-

E

E

N

D

H

T

P

I

T

G

D

K

P

P

L

L

C

S

S

P

Y
|
Y

G

A

I

V

V

T

K
|
K

L

Y

A

V

V

N

E

E

K

L

Y

Y

L

A

A

D

L

V

Y

F

H

S

E

R

L

C

Y

Y

G

G

I

F

D

S

Y

T

A

I

A

G

L

L

R

R

I
x
Y

S

F

N
|
N

P
x
V

F

F

G

G

P

R

L

R

Q

Q

R

D

-

P

T
x
N

S

G

A

A

E

Y

Q

A

G
x
A

V
|
V

I

I

G

P

V

K

F
x
W

L

T

G

S

K

S

I

M

L

I

R

Q

N

G

E

D

P

D

L

V

H
x
Y

V
x
I

W
x
N

G

G

D

D

G

G

S

E

V

T

V

S

R

R

D

D

Y

F

I

C

Y

Y

V

I

E

E

D

N

V

T

A

V

R

Q

A

A

L

N

V

L

L

L

A

A

A

A

R

T

-

A

-

G

L

L

N

D

T

A

E

R

H

N

H

Q

V

V

F

Y

N

N

I

I

G

A

S

V

G

G

A

G

G

R

L

T

S

S

L
|
L

N

N

D

Q

I

L

I

F

D

F

M

A

M

L

R

R

S

D

V

G

T

L

G

A

R

E

D

N

V

G

V

V

V

S

K

Y

-

H

-

R

-

E

-

P

-

V

Y

Y

D

R

Q

D

N

F

R
|
R

V

E

F

G

D
|
D

V

V

P

R

Y

H

S

S

V

L

L

A

D

D

V

I

S

S

L

K

A

A

E

A

R

K

E

L

L

L

G

G

W

Y

R

A

A

P

L

K

F

Y

P

D

F

V

K

S

V

A

G

G

V

V

S

A

L

L

A

A

W

M

V

P

W

W

active site: S141 (= S121), S142 (≠ G123), S143 (≠ A124), Y165 (= Y146), K169 (= K150), N203 (≠ T183)
binding nicotinamide-adenine-dinucleotide: G22 (= G10), G25 (= G13), F26 (= F14), I27 (≠ L15), D46 (≠ H31), N47 (≠ A32), F48 (= F33), T50 (≠ R35), G51 (≠ D36), D77 (= D57), I78 (≠ F58), Q97 (≠ L77), A99 (≠ S79), T116 (≠ E96), A139 (= A119), A140 (≠ S120), Y165 (= Y146), K169 (= K150), Y192 (≠ I173), N194 (= N175), V195 (≠ P176)
binding uridine-5'-diphosphate-glucose: S141 (= S121), Y165 (= Y146), N194 (= N175), A208 (≠ G188), V209 (= V189), W213 (≠ F193), Y224 (≠ H204), I225 (≠ V205), N226 (≠ W206), L270 (= L248), R298 (= R271), D301 (= D274)

1sb8A Crystal structure of pseudomonas aeruginosa udp-n-acetylglucosamine 4- epimerase complexed with udp-n-acetylgalactosamine (see paper)
30% identity, 96% coverage: 8:307/312 of query aligns to 21:335/341 of 1sb8A

query
sites
1sb8A

V

I

L

T

G
|
G

A

V

S

A

G
|
G

F
|
F

L
x
I

G

G

S

S

H

N

L

L

V

L

H

E

H

T

L

L

L

L

K

K

A

L

G

D

C

Q

Q

K

V

V

-

V

-

G

-

L

H
x
D

A
x
N

F
|
F

S

A

R
x
T

D
x
G

S

H

R

Q

R

R

N

N

-

L

-

D

-

E

-

V

-

R

P

S

L

L

L

V

T

S

D

E

E

K

L

Q

M

W

S

S

S

N

C

F

S

K

I

F

F

I

T

Q

G

G

D
|
D

F
x
I

F

R

N

N

T

L

Q

D

D

D

V

C

E

N

R

N

A

A

L

C

A

A

D

G

C

V

D

D

V

Y

C

V

F

L

H

H

L
x
Q

V

A

S
x
A

T

L

-

G

T
x
S

I

V

P

P

K

R

T

S

S

I

N

N

D

D

D

-

P

P

L

I

R

T

D

S

V

N

R

A

E
x
T

N

N

L

I

S

D

G

G

S

F

L

L

T

N

L

M

L

L

E

I

C

A

V

A

R

R

R

D

T

A

G

K

V

V

R

Q

K

S

V

F

V

T

Y

Y

A
|
A

S
x
A

S
|
S

G

-

G
x
S

A
x
S

I

T

Y

Y

G

G

K

D

H

H

L

P

-

G

M

L

P

P

R

K

I

V

S

-

E

E

N

D

H

T

P

I

T

G

D

K

P

P

L

L

C

S

S

P

Y
|
Y

G

A

I

V

V

T

K
|
K

L

Y

A

V

V

N

E

E

K

L

Y

Y

L

A

A

D

L

V

Y

F

H

S

E

R

L

C

Y

Y

G

G

I

F

D

S

Y

T

A

I

A

G

L

L

R

R

I
x
Y

S

F

N
|
N

P
x
V

F

F

G

G

P

R

L

R

Q

Q

R

D

-

P

T
x
N

S

G

A

A

E

Y

Q

A

G
x
A

V
|
V

I

I

G

P

V

K

F
x
W

L

T

G

S

K

S

I

M

L

I

R

Q

N

G

E

D

P

D

L

V

H
x
Y

V
x
I

W
x
N

G

G

D

D

G

G

S

E

V

T

V

S

R
|
R

D

D

Y

F

I

C

Y

Y

V

I

E

E

D

N

V

T

A

V

R

Q

A

A

L

N

V

L

L

L

A

A

A

A

R

T

-

A

-

G

L

L

N

D

T

A

E

R

H

N

H

Q

V

V

F

Y

N

N

I

I

G

A

S

V

G

G

A

G

G

R

L

T

S

S

L
|
L

N

N

D

Q

I

L

I

F

D

F

M

A

M

L

R

R

S

D

V

G

T

L

G

A

R

E

D

N

V

G

V

V

V

S

K

Y

-

H

-

R

-

E

-

P

-

V

Y

Y

D

R

Q

D

N

F

R
|
R

V

E

F

G

D
|
D

V

V

P

R

Y

H

S
|
S

V

L

L

A

D

D

V

I

S

S

L

K

A

A

E

A

R

K

E

L

L

L

G

G

W

Y

R

A

A

P

L

K

F

Y

P

D

F

V

K

S

V

A

G

G

V

V

S

A

L

L

A

A

W

M

V

P

W

W

active site: S142 (= S121), S143 (≠ G123), S144 (≠ A124), Y166 (= Y146), K170 (= K150), N204 (≠ T183)
binding nicotinamide-adenine-dinucleotide: G23 (= G10), G26 (= G13), F27 (= F14), I28 (≠ L15), D47 (≠ H31), N48 (≠ A32), F49 (= F33), T51 (≠ R35), G52 (≠ D36), D78 (= D57), I79 (≠ F58), Q98 (≠ L77), A100 (≠ S79), T117 (≠ E96), A140 (= A119), A141 (≠ S120), Y166 (= Y146), K170 (= K150), Y193 (≠ I173), N195 (= N175), V196 (≠ P176)
binding uridine-diphosphate-n-acetylgalactosamine: S103 (≠ T81), S142 (= S121), S143 (≠ G123), S144 (≠ A124), Y166 (= Y146), N195 (= N175), A209 (≠ G188), V210 (= V189), W214 (≠ F193), Y225 (≠ H204), I226 (≠ V205), N227 (≠ W206), R234 (= R213), L271 (= L248), R299 (= R271), D302 (= D274), S306 (= S278)

6kvcA Moee5 in complex with udp-glucose and NAD (see paper)
33% identity, 96% coverage: 8:307/312 of query aligns to 5:293/299 of 6kvcA

query
sites
6kvcA

V

V

L

T

G
|
G

A

A

S

A

G
|
G

F
|
F

L
x
I

G

G

S

S

H

H

L

L

V

V

H

T

H

E

L

L

L

R

K

N

A

S

G

G

C

R

Q

N

V

V

H

V

A

A

F

V

S
x
D

R
|
R

D

-

S

-

R

-

R

-

N
x
R

P

P

L

L

L

P

T

T

D

G

E

S

L

L

M

R

S

E

S

-

C

-

S

-

I

-

F

I

T

R

G

G

D
|
D

F
x
L

F

-

N

N

T

S

Q

L

D

N

V

L

E

V

R

D

A

C

L

L

A

K

D

N

C

I

D

S

V

T

C

V

F

F

H

H

L
|
L

V
x
A

S
x
A

T

L

T
x
P

I

G

P

V

K
x
R

T

P

S

S

N

W

D

T

D

Q

P

F

L

P

R

E

D

Y

V

L

R

R

E

C

N

N

L

V

S

L

G

A

S

T

L

Q

T

R

L

L

L

M

E

E

C

A

V

C

R

V

R

Q

T

A

G

G

V

V

R

E

K

R

V

V

V

V

Y

V

A
|
A

S

S

S
|
S

G
x
S

G
x
S

A

V

I

Y

Y

G

G

G

K

A

H

D

L

G

M

V

P

-

R

-

I

M

S

S

E

E

N

D

H

D

P

L

T

P

D

R

P

P

L

L

C

S

S

P

Y
|
Y

G

G

I

V

V

T

K
|
K

L

L

A

A

V

A

E

E

K

R

Y

-

L

L

A

A

L

L

Y

A

H

F

E

A

L

A

Y

R

G

G

-

D

-

A

-

E

I

L

D

S

Y

V

A

G

A

A

L

L

R

R

I
x
F

S

F

N
x
T

P
x
V

F

Y

G

G

P

P

L

G

Q

Q

R
|
R

T

P

S

D

A
x
M

E

-

Q

-

G

-

V
x
F

I

I

G

S

V
x
R

F

L

L

I

G

R

K

A

I

T

L

L

R

R

N

G

E

E

P

P

L

V

H
x
E

V
x
I

W
x
Y

G

G

D

D

G

G

S

T

V
x
Q

V

L

R
|
R

D

D

Y

F

I

T

Y

H

V

V

E

S

D

D

V

V

A

V

R

R

A

A

L

L

V

M

L

L

A

T

A

A

R

S

L

V

N

R

T

D

E

R

-

G

H

S

H

A

V

V

F

L

N

N

I

I

G

G

S

T

G

G

A

S

G

A

L

V

S

S

L

V

N

N

D

E

I

V

I

V

D

S

M

M

M

T

R

A

S

E

V

L

T

T

G

G

R

L

D

R

V

P

V

C

V

T

K

A

Y

Y

D

G

Q

S

N

A

R

R

V

I

F

G

D
|
D

V

V

P

R

Y

S

S

T

V

T

L

A

D

D

V

V

S

R

L

Q

A

A

E

Q

R

S

E

V

L

L

G

G

W

F

R

T

A

A

L

R

F

T

P

G

F

L

K

R

V

E

G

G

V

L

S

A

L

T

A

Q

W

I

V

E

W

W

active site: S109 (= S121), S110 (≠ G122), S111 (≠ G123), Y132 (= Y146), K136 (= K150)
binding nicotinamide-adenine-dinucleotide: G7 (= G10), G10 (= G13), F11 (= F14), I12 (≠ L15), D31 (≠ S34), R32 (= R35), R33 (≠ N40), D46 (= D57), L47 (≠ F58), L65 (= L77), A66 (≠ V78), A67 (≠ S79), P69 (≠ T81), A107 (= A119), S109 (= S121), K136 (= K150), F161 (≠ I173), T163 (≠ N175), V164 (≠ P176), R170 (= R182), M173 (≠ A185)
binding uridine-5'-diphosphate-glucose: P69 (≠ T81), R72 (≠ K84), S109 (= S121), S110 (≠ G122), Y132 (= Y146), T163 (≠ N175), M173 (≠ A185), F174 (≠ V189), R177 (≠ V192), E189 (≠ H204), I190 (≠ V205), Y191 (≠ W206), Q196 (≠ V211), R198 (= R213), D260 (= D274)

6pmhA Structure of a udp-gale 4-epimerase (mth375) from the thermophilic pseudomurein-containing methanogen methanothermobacter thermautotrophicus
29% identity, 100% coverage: 1:311/312 of query aligns to 1:320/330 of 6pmhA

query
sites
6pmhA

M

M

S

D

S

E

F

F

R

R

A

A

-

Y

-

D

-

G

-

K

C

C

V

V

L

L

-

V

-

T

G
|
G

A

G

S

A

G
|
G

F

C

L

V

G

G

S

S

H

N

L

L

V

T

H

G

H

N

L

L

L

A

K

K

A

A

G

G

C

A

Q

N

V

V

H

I

A

I

F

L

S
x
D

R
x
N

D

L

S
|
S

R
x
S

R
x
S

N

Y

P

E

L

W

L

N

T

I

D

P

E

E

L

-

M

Y

S

E

S

N

C

I

S

E

I

F

F

V

T

K

G

G

D
|
D

F
x
I

F

L

N

D

T

D

Q

E

D

V

V

L

E

K

R

R

A

V

L

F

A

K

D

E

-

R

C

P

D

D

V

Y

C

V

F

F

H

H

L

L

V
x
A

S

A

T

H

T
x
F

I

A

P

N

K

Q

T

N

S

S

N

V

D

D

D

N

P

P

L

E

R

K

D

D

V

L

R

L

E

V

N

N

L

G

S

L

G

G

S

I

L

L

T

K

L

V

L

L

E

E

C

Y

V

A

R

Q

R

L

T

V

G

G

V

V

R

E

K

R

V

F

V

V

Y

Y

A

S

S

S

S
|
S

G

G

G
x
C

A
x
G

I

V

Y

Y

G

G

K

L

H

D

L

S

M

K

P

I

R

P

I

F

S

E

E

E

N

H

H

D

P

I

T

S

D

I

P

S

L

L

-

H

C

T

S

P

Y
|
Y

G

Q

I

V

V

T

K
|
K

L

L

A

L

V

G

E

E

K

L

Y

Y

L

T

A

N

L

Y

Y

F

H

H

E

N

L

L

Y

Y

G

E

I

M

D

P

Y

I

A

V

A

N

L

A

R

R

I

F

S

F

N
|
N

P

V

F

F

G

G

P

P

L

G

Q

E

R

V

T

P

S

G

A

K

E

Y

Q
x
R

G
x
N

V
|
V

I

I

G

P

V

N

F
|
F

L

F

G

Y

K

W

I

A

L

M

R

N

N

Q

E

Q

P

P

L

L

H
x
P

V
x
I

W
x
T

G

G

D

D

G

G

S

S

V

E

V

T

R
|
R

D

D

Y

W

I

T

Y

F

V

V

E

E

D

D

V

I

A

V

R

R

A

G

L

L

V

M

-

A

L

M

A

G

A

V

R

R

L

R

N

E

T

A

E

I

H

G

H

E

V

A

F

I

N

N

I

L

G

G

S

S

G

G

A

T

G

E

L

H

S

Q

L
x
V

N

I

D

E

I

M

I

A

D

G

M

I

M

I

R

N

S

E

V

L

T

T

G

E

R

N

D

P

V

A

V

G

V

V

K

V

Y

Y

D

R

Q

P

N

R

R
|
R

V

D

F

W

D

D

V

A

P

K

Y

T

S

R

V

L

L

L

-

S

D

S

V

I

S

D

L

K

A

A

E

R

R

R

E

L

L

L

G

D

W

Y

R

E

A

P

L

Q

F

V

P

S

F

F

K

R

V

E

G

G

V

L

S

E

L

R

A

T

W

H

V

R

W

W

L

F

C

T

E

E

N

N

active site: S127 (= S121), C129 (≠ G123), G130 (≠ A124), Y153 (= Y146), K157 (= K150)
binding adenosine monophosphate: G16 (= G10), G19 (= G13), D40 (≠ S34), N41 (≠ R35), S43 (= S37), S44 (≠ R38), S45 (≠ R39), D62 (= D57), I63 (≠ F58), A84 (≠ V78), F87 (≠ T81), R194 (≠ Q187)
binding uridine-5'-diphosphate: C129 (≠ G123), N182 (= N175), N195 (≠ G188), V196 (= V189), F200 (= F193), P211 (≠ H204), I212 (≠ V205), T213 (≠ W206), R220 (= R213), V256 (≠ L248), R279 (= R271)

6pnlA Structure of a udp-gale 4-epimerase (mth375) from the thermophilic pseudomurein-containing methanogen methanothermobacter thermautotrophicus
29% identity, 100% coverage: 1:311/312 of query aligns to 7:326/336 of 6pnlA

query
sites
6pnlA

M

M

S

D

S

E

F

F

R

R

A

A

-

Y

-

D

-

G

-

K

C

C

V

V

L

L

-

V

-

T

G
|
G

A

G

S

A

G
|
G

F
x
C

L

V

G

G

S

S

H

N

L

L

V

T

H

G

H

N

L

L

L

A

K

K

A

A

G

G

C

A

Q

N

V

V

H

I

A

I

F

L

S
x
D

R
x
N

D
x
L

S
|
S

R
x
S

R
x
S

N

Y

P

E

L

W

L

N

T

I

D

P

E

E

L

-

M

Y

S

E

S

N

C

I

S

E

I

F

F

V

T

K

G

G

D
|
D

F
x
I

F

L

N

D

T

D

Q

E

D

V

V

L

E

K

R

R

A

V

L

F

A