SitesBLAST

O52681 Bifurcating [FeFe] hydrogenase gamma subunit; Hydrogenase (NAD(+), ferredoxin) gamma subunit; Iron-hydrogenase gamma subunit; EC 1.12.1.4 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
42% identity, 93% coverage: 12:158/158 of query aligns to 6:152/161 of O52681

query
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O52681

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D79 (≠ M85) mutation to P: Increase (30 mV) in the redox potential.
G80 (= G86) mutation to P: Large decrease (149 mV) in the redox potential. Causes a change to a [4Fe-4S] cluster protein.
T81 (= T87) mutation to P: Small increase (11 mV) in the redox potential.
A82 (= A88) mutation to P: Decrease (46 mV) in the redox potential.
L120 (≠ V126) mutation to P: Large decrease (65 mV) in the redox potential.
G121 (= G127) mutation to P: Decrease (22 mV) in the redox potential.
A122 (= A128) mutation to P: Large decrease (132 mV) in the redox potential.
V128 (= V134) mutation V->A,C,H,Y: Small increase (about 2-14 mV) in the redox potential.; mutation V->D,G,R,S,T,W: Increase (about 23-33 mV) in the redox potential.; mutation V->E,K,L,M: Decrease (about 37-50 mV) in the redox potential.; mutation V->F,I,P,Q: Small decrease (about 4-20 mV) in the redox potential.; mutation to N: Large increase (85 mV) in the redox potential.

7p5hC Tmhydabc- d2 map (see paper)
42% identity, 93% coverage: 12:158/158 of query aligns to 3:149/156 of 7p5hC

query
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7p5hC

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binding fe2/s2 (inorganic) cluster: C75 (= C84), G77 (= G86), A79 (= A88), C80 (= C89), C116 (= C125), L117 (≠ V126), A119 (= A128), C120 (= C129)

2auvA Solution structure of hndac : a thioredoxin-like [2fe-2s] ferredoxin involved in the NADP-reducing hydrogenase complex (see paper)
62% identity, 53% coverage: 73:156/158 of query aligns to 1:84/85 of 2auvA

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2auvA

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binding fe2/s2 (inorganic) cluster: C12 (= C84), C17 (= C89), C53 (= C125), V54 (= V126), G55 (= G127), C57 (= C129)

8oh5A Cryo-em structure of the electron bifurcating transhydrogenase stnabc complex from sporomusa ovata (state 2) (see paper)
41% identity, 87% coverage: 20:157/158 of query aligns to 12:149/150 of 8oh5A

query
sites
8oh5A

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binding fe2/s2 (inorganic) cluster: C76 (= C84), C81 (= C89), C117 (= C125), L118 (≠ V126), G119 (= G127), C120 (≠ A128), C121 (= C129)

5gupE structure of mammalian respiratory supercomplex I1III2IV1 (see paper)
41% identity, 87% coverage: 21:158/158 of query aligns to 50:187/197 of 5gupE

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sites
5gupE

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binding fe2/s2 (inorganic) cluster: C113 (= C84), T115 (≠ G86), P117 (≠ A88), C118 (= C89), C154 (= C125), L155 (≠ V126), G156 (= G127), A157 (= A128), C158 (= C129), M163 (≠ V134)

7dgq9 NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (see paper)
41% identity, 87% coverage: 21:158/158 of query aligns to 33:170/207 of 7dgq9

query
sites
7dgq9

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binding fe2/s2 (inorganic) cluster: C96 (= C84), T98 (≠ G86), C101 (= C89), C137 (= C125), L138 (≠ V126), A140 (= A128), C141 (= C129)
binding : H35 (≠ N23), R49 (≠ H37)

Sites not aligning to the query:

binding : 20, 23, 29, 32

7v2cO Active state complex i from q10 dataset (see paper)
41% identity, 87% coverage: 21:158/158 of query aligns to 40:177/217 of 7v2cO

query
sites
7v2cO

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binding fe2/s2 (inorganic) cluster: C103 (= C84), P107 (≠ A88), C108 (= C89), C144 (= C125), L145 (≠ V126), G146 (= G127), A147 (= A128), C148 (= C129)

8eswV2 NADH dehydrogenase (Ubiquinone) 24 kDa subunit, isoform A (see paper)
41% identity, 87% coverage: 22:158/158 of query aligns to 38:174/214 of 8eswV2

query
sites
8eswV2

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binding fe2/s2 (inorganic) cluster: C100 (= C84), C105 (= C89), C141 (= C125), L142 (≠ V126), G143 (= G127), C145 (= C129)
binding : R53 (≠ H37)

Sites not aligning to the query:

binding : 27, 30, 37

8b9zE Drosophila melanogaster complex i in the active state (dm1) (see paper)
41% identity, 87% coverage: 22:158/158 of query aligns to 38:174/214 of 8b9zE

query
sites
8b9zE

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I

K

I

V

A

A

S

E

K

I

L

L

R

Q

I

L

P

P

V

N

S

M

K

R

V

V

Y

Y

G

E

V

V

V

A

T

T

F

F

Y

Y

T

T

F

M

F

F

T

M

M

R

T

K

P

P

K

T

G

G

K

K

H

Y

P

H

I

I

S

Q

V

V

C
|
C

M

T

G
x
T

T
|
T

A
x
P

C
|
C

Y

W

V

L

R

R

G

G

S

S

E

D

K

D

L

I

L

L

E

E

E

T

F

C

K

K

R

K

V

Q

L

L

G

G

I

I

E

G

V

V

G

G

E

D

T

T

P

K

D

D

G

R

K

K

Y

F

S

T

L

I

D

S

C

E

L

V

R

E

C
|
C

V
x
L

G
|
G

A
|
A

C
|
C

G

V

L

N

A

A

P

P

V

M

V

V

M

A

I

I

G

N

E

D

K

D

V

Y

Y

Y

G

E

R

D

L

L

Q

T

P

S

V

K

D

D

V

M

K

Q

K

D

I

I

I

L

E

N

E

D

L

L

E

K

binding fe2/s2 (inorganic) cluster: C100 (= C84), T102 (≠ G86), T103 (= T87), P104 (≠ A88), C105 (= C89), C141 (= C125), L142 (≠ V126), G143 (= G127), A144 (= A128), C145 (= C129)

P19404 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; NADH-ubiquinone oxidoreductase 24 kDa subunit; EC 7.1.1.2 from Homo sapiens (Human) (see 2 papers)
41% identity, 87% coverage: 22:158/158 of query aligns to 73:209/249 of P19404

query
sites
P19404

G

G

N

H

N

K

A

A

G

A

E

A

L

V

I

L

N

P

I

V

L

L

H

D

E

L

A

A

Q

Q

H

R

L

Q

H

N

G

G

Y

W

L

L

P

P

E

I

E

S

M

A

Q

M

R

N

I

K

I

V

A

A

S

E

K

V

L

L

R

Q

I

V

P

P

V

P

S

M

K

R

V

V

Y

Y

G

E

V

V

V

A

T

T

F

F

Y

Y

T

T

F

M

F

Y

T

N

M

R

T

K

P

P

K

V

G

G

K

K

H

Y

P

H

I

I

S

Q

V

V

C

C

M

T

G

T

T

T

A

P

C

C

Y

M

V

L

R

R

G

N

S

S

E

D

K

S

L

I

L

L

E

E

E

A

F

I

K

Q

R

K

V

K

L

L

G

G

I

I

E

K

V

V

G

G

E

E

T

T

P

P

D

D

G

K

K

L

Y

F

S

T

L

L

D

I

C

E

L

V

R

E

C

C

V

L

G

G

A

A

C

C

G

V

L

N

A

A

P

P

V

M

V

V

M

Q

I

I

G

N

E

D

K

N

V

Y

Y
|
Y

G

E

R

D

L

L

Q

T

P

A

V

K

D

D

V

I

K

E

I

I

E

D

E

E

L

L

E

K

Y193 (= Y142) modified: Phosphotyrosine; by SRC

Sites not aligning to the query:

29 V → A: in dbSNP:rs906807

6vw8C Formate dehydrogenase fdsabg subcomplex fdsbg from c. Necator (see paper)
38% identity, 84% coverage: 26:157/158 of query aligns to 18:149/152 of 6vw8C

query
sites
6vw8C

G

G

E

A

L

L

I

L

N

P

I

I

L

L

H

H

E

E

A

I

Q

Q

H

D

L

T

H

Q

G

G

Y

Y

L

I

P

P

E

D

E

A

M

A

Q

V

R

P

I

V

I

I

A

A

S

R

K

A

L

L

R

N

I

L

P

S

V

R

S

A

K

E

V

V

Y

H

G

G

V

V

V

I

T

T

F

F

Y

Y

T

H

F

H

F

F

T

R

M

Q

T

Q

P

P

K

A

G

G

K

R

H

H

P

V

I

V

S

Q

V

V

C
|
C

M

R

G

A

T

E

A
|
A

C
|
C

Y

Q

V

S

R

V

G

G

S

A

E

E

K

A

L

L

L

A

E

E

E

H

F

A

K

Q

R

R

V

A

L

L

G

G

I

C

E

G

V

F

G

H

E

E

T

T

P

A

D

D

G

G

K

Q

Y

V

S

T

L

L

D

E

C

P

L

V

R

Y

C
|
C

V
x
L

G
|
G

A
x
Q

C
|
C

G

A

L

C

A

G

P

P

V

A

V

V

M

M

I

V

G

G

E

E

K

Q

V

L

Y

H

G

G

R

Y

L

V

Q

D

P

A

V

R

D

R

V

F

K

D

K

A

I

L

I

V

E

R

E

S

L

L

binding fe2/s2 (inorganic) cluster: C76 (= C84), A80 (= A88), C81 (= C89), C117 (= C125), L118 (≠ V126), G119 (= G127), Q120 (≠ A128), C121 (= C129)

6r7pA Crystal structure of oxidized aquifex aeolicus nadh-quinone oxidoreductase subunits nuoe and nuof s96m (see paper)
37% identity, 91% coverage: 12:154/158 of query aligns to 7:153/157 of 6r7pA

query
sites
6r7pA

E

E

Q

E

V

L

K

K

T

T

I

K

C

L

D

Q

K

E

H

H

G

I

N

N

-

Y

-

F

-

P

-

K

N

K

A

R

G

Q

E

A

L

I

I

L

N

L

I

C

L

L

H

H

E

E

A

I

Q

Q

H

N

L

Y

H

Y

G

G

Y

Y

L

I

P

P

E

P

E

E

M

S

Q

L

R

K

I

P

I

L

A

A

S

D

K

M

L

L

R

E

I

L

P

P

V

L

S

N

K

H

V

V

Y

E

G

G

V

V

T

A

F

F

Y

Y

T

D

F

M

F

F

T

D

M

R

T

E

P

D

K

K

G

A

K

K

H

Y

P

R

I

I

S

R

V

V

C
|
C

M

V

G

S

T

I

A
x
V

C
|
C

Y

H

V

L

R

M

G

G

S

T

E

N

K

K

L

L

E

K

E

A

F

L

K

E

R

N

V

I

L

L

G

G

I

I

E

K

V

P

G

G

E

E

T

V

T

T

P

P

D

D

G

G

K

K

Y

F

S

K

L

I

D

V

C

P

L

V

R

Q

C
|
C

V
x
L

G

G

A
|
A

C
|
C

G

S

L

E

A

A

P

P

V

V

V

F

M

M

I

V

G

N

E

D

K

D

V

E

Y

Y

G

K

R

F

L

E

Q

S

P

E

V

V

D

Q

V

L

K

N

K

E

I

I

I

L

binding fe2/s2 (inorganic) cluster: C83 (= C84), V87 (≠ A88), C88 (= C89), C124 (= C125), L125 (≠ V126), A127 (= A128), C128 (= C129)

6tg9G Cryo-em structure of nadh reduced form of NAD+-dependent formate dehydrogenase from rhodobacter capsulatus (see paper)
32% identity, 92% coverage: 13:157/158 of query aligns to 5:147/148 of 6tg9G

query
sites
6tg9G

Q

R

V

L

K

R

T

A

I

I

C

L

D

A

K

A

H

H

G

R

N

G

N

R

A

E

G

G

E

A

L

L

I

L

N

P

I

I

L

L

H

H

E

D

A

V

Q

Q

H

A

L

A

H

F

G

G

Y

F

L

I

P

P

E

E

E

D

M

A

Q

Y

R

A

I

P

I

I

A

A

S

A

K

D

L

L

R

G

I

L

P

T

V

R

S

A

K

E

V

V

Y

A

G

G

V

V

T

G

F

F

Y

Y

T

H

F

D

F

F

T

R

M

K

T

A

P

P

K

A

G

G

K

R

H

H

P

V

I

I

S

K

V

L

C
|
C

M

R

G
x
A

T

E

A

A

C
|
C

Y

Q

V

A

R

M

G

G

S

M

E

D

K

A

L

V

L

Q

E

A

E

R

F

L

K

E

R

S

V

A

L

L

G

G

I

L

E

R

V

L

G

G

E

D

T

S

P

E

D

-

G

-

K

A

Y

V

S

T

L

L

D

E

C

A

L

V

R

Y

C
|
C

V
x
L

G

G

A
x
L

C
|
C

G

A

L

C

A

A

P

P

V

A

M

M

I

V

G

D

E

D

K

R

V

L

Y

V

G

G

R

R

L

L

Q

D

P

A

V

A

D

A

V

V

K

A

K

G

I

I

I

V

E

A

E

E

L

L

binding fe2/s2 (inorganic) cluster: C76 (= C84), A78 (≠ G86), C81 (= C89), C115 (= C125), L116 (≠ V126), L118 (≠ A128), C119 (= C129)

8e9gE Mycobacterial respiratory complex i with both quinone positions modelled (see paper)
31% identity, 94% coverage: 9:157/158 of query aligns to 23:175/233 of 8e9gE

query
sites
8e9gE

D

D

V

V

V

T

E

E

Q

S

V

L

K

R

T

A

-

D

-

A

-

E

-

Q

I

I

C

I

D

A

K

R

H

Y

G

P

N

D

N

A

A

R

G

S

E

A

L

L

I

L

N

P

I

L

L

L

H

H

E

L

A

V

Q

Q

H

A

L

Q

H

D

G

G

Y

Y

L

L

P

T

E

P

E

A

M

G

Q

I

R

G

I

F

I

C

A

A

S

A

K

Q

L

L

R

G

I

L

P

T

V

E

S

A

K

E

V

V

Y

T

G

A

V

V

V

A

T

T

F

F

Y

Y

T

S

F

M

F

Y

T

R

M

R

T

T

P

P

K

T

G

G

K

D

H

Y

P

L

I

V

S

G

V

V

C
|
C

M

T

G

N

T

T

A

L

C
|
C

Y

A

V

I

R

M

G

G

S

G

E

D

K

A

L

I

L

L

E

E

E

A

F

L

K

E

R

D

V

H

L

L

G

G

I

V

E

H

V

P

G

G

E

Q

T

T

P

P

D

D

G

G

K

R

Y

V

S

T

L

L

D

E

C

H

L

V

R

E

C
|
C

V
x
N

G
x
A

A
|
A

C
|
C

G

D

L

Y

A

A

P

P

V
|
V

V

V

M

M

I

V

G

N

E

W

K

E

V

F

Y

Y

G

D

R

N

L

Q

Q

T

P

P

V

S

D

S

V

A

K

R

K

D

I

L

I

V

E

D

E

G

L

L

binding fe2/s2 (inorganic) cluster: C102 (= C84), C107 (= C89), C143 (= C125), N144 (≠ V126), A145 (≠ G127), A146 (= A128), C147 (= C129), V152 (= V134)

7zmbH Cryoem structure of mitochondrial complex i from chaetomium thermophilum (state 2) (see paper)
35% identity, 83% coverage: 28:158/158 of query aligns to 45:177/221 of 7zmbH

query
sites
7zmbH

L

V

I

M

N

P

I

L

L

L

H

D

E

L

A

G

Q

Q

H

R

L

Q

H

H

G

G

Y

F

L

C

P

S

E

I

E

S

M

V

Q

M

R

N

I

E

I

V

A

A

S

R

K

I

L

L

R

E

I

M

P

P

V

P

S

M

K

R

V

V

Y

Y

G

E

V

V

V

A

T

S

F

F

Y

Y

T

T

F

M

F

Y

T

N

M

R

T

T

P

P

K

V

G

G

K

K

H

F

P

H

I

V

S

Q

V

A

C
|
C

M

T

G

T

T

T

A
x
P

C
|
C

Y

Q

V

L

R

G

G

G

-

C

-

G

S

S

E

D

K

A

L

I

L

V

E

K

E

A

F

I

K

K

R

E

V

H

L

L

G

G

I

I

E

N

V

Q

G

G

E

E

T

T

P

P

D

D

G

G

K

L

Y

F

S

T

L

F

D

I

C

E

L

V

R

E

C
|
C

V

L

G

G

A
|
A

C
|
C

G

V

L

N

A

A

P

P

V

M

V

V

M

Q

I

I

G

N

E

D

K

D

V

Y

Y

Y

G

E

R

D

L

L

Q

T

P

P

V

E

D

T

V

I

K

K

K

Q

I

V

I

L

E

T

E

A

L

L

E

K

binding fe2/s2 (inorganic) cluster: C101 (= C84), P105 (≠ A88), C106 (= C89), C144 (= C125), A147 (= A128), C148 (= C129)

7b0nE 3.7-angstrom structure of Yarrowia lipolytica complex I with an R121M mutation in NUCM. (see paper)
34% identity, 80% coverage: 31:156/158 of query aligns to 47:172/216 of 7b0nE

query
sites
7b0nE

I

L

L

L

H

D

E

I

A

G

Q

Q

H

R

L

Q

H

L

G

G

Y

Y

L

T

P

S

E

I

E

S

M

V

Q

M

R

N

I

Y

I

V

A

A

S

K

K

L

L

L

R

E

I

M

P

P

V

P

S

M

K

R

V

V

Y

Y

G

E

V

V

V

A

T

T

F

F

Y

Y

T

T

F

M

F

Y

T

N

M

R

T

T

P

P

K

M

G

G

K

R

H

Y

P

H

I

L

S

Q

V

I

C
|
C

M

T

G
x
T

T

T

A
x
P

C
|
C

Y

Q

V

L

R

C

G

G

S

S

E

D

K

G

L

I

L

M

E

E

E

A

F

V

K

Q

R

N

V

T

L

L

G

N

I

I

E

K

V

P

G

G

E

E

T

T

P

K

D

D

G

N

K

L

Y

F

S

T

L

L

D

S

C

E

L

V

R

E

C
|
C

V
x
L

G

G

A
|
A

C
|
C

G

V

L

N

A

A

P

P

V

M

M

A

I

I

G

N

E

D

K

D

V

Y

Y

Y

G

E

R

D

L

L

Q

T

P

P

V

E

D

G

V

T

K

V

K

K

I

L

E

E

E

D

binding fe2/s2 (inorganic) cluster: C100 (= C84), T102 (≠ G86), P104 (≠ A88), C105 (= C89), C141 (= C125), L142 (≠ V126), A144 (= A128), C145 (= C129)

7arcE Cryo-em structure of polytomella complex-i (peripheral arm) (see paper)
32% identity, 85% coverage: 25:158/158 of query aligns to 45:189/235 of 7arcE

query
sites
7arcE

A

A

G

S

E

A

L

M

I

I

N

P

I

L

L

L

H

D

E

L

A

A

Q

Q

H

Q

L

Q

H

N

G

G

-

G

Y

V

L

V

P

S

E

L

E

A

M

V

Q

M

R

N

I

R

I

V

A

A

S

Q

K

I

L

L

R

E

I

V

P

P

V

P

S

I

K

K

V

V

Y

Y

G

E

V

V

V

A

T

T

F

F

Y

F

T

T

F

M

F

F

T

N

M

R

T

S

P

K

K

M

G

G

K

K

H

Y

P

H

I

V

S

C

V

I

C
|
C

M

G

G

T

T

T

A
x
P

C
|
C

Y

R

V

L

R

Q

G

G

S

A

E

Q

K

K

L

I

L

E

E

E

E

A

F

I

K

T

R

K

V

H

L

L

G

G

I

V

E

G

V

I

G

G

E

Q

T

T

P

A

D

D

G

G

K

T

Y

F

S

T

L

L

D

G

C

E

L

M

R

E

C
|
C

V
x
M

G

G

A
|
A

C
|
C

G

V

L

N

A

A

P

P

V

M

V

I

M

A

I

V

G

A

E

D

-

Y

-

R

-

N

-

G

-

V

-

E

-

G

-

F

-

S

-

Y

K

N

V

Y

Y

Y

G

E

R

D

L

L

Q

T

P

P

V

Q

D

D

V

A

K

V

K

N

I

I

I

L

E

E

E

K

L

L

E

K

binding fe2/s2 (inorganic) cluster: C105 (= C84), P109 (≠ A88), C110 (= C89), C146 (= C125), M147 (≠ V126), A149 (= A128), C150 (= C129)

Query Sequence

>349651 FitnessBrowser__Btheta:349651
MSDIKLACDVVEQVKTICDKHGNNAGELINILHEAQHLHGYLPEEMQRIIASKLRIPVSK
VYGVVTFYTFFTMTPKGKHPISVCMGTACYVRGSEKLLEEFKRVLGIEVGETTPDGKYSL
DCLRCVGACGLAPVVMIGEKVYGRLQPVDVKKIIEELE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory