SitesBLAST

1hm9A Crystal structure of s.Pneumoniae n-acetylglucosamine-1-phosphate uridyltransferase, glmu, bound to acetyl coenzyme a and udp-n- acetylglucosamine (see paper)
39% identity, 38% coverage: 116:191/201 of query aligns to 346:437/458 of 1hm9A

query
sites
1hm9A

C

V

E

E

I

V

N

K

A

G

K

S

S

S

I

I

G

G

K

E

N

N

F

T

S

K

C

A

R

G

H

H

L

L

T

T

T
x
Y

L

I

G

G

N

N

-

C

-

E

-

V

-

G

-

S

-

N

-

V

-

N

-

F

-

G

-

A

-

G

-

T

-

I

-

T

-
x
V

K
x
N

A
x
Y

D

D

G

G

D

K

N

N

D

K

N

Y

R

K

P

T

V

V

I

I

G

G

D
|
D

N

N

V

V

T

F

L

V

G

G

V

S

N

N

V

S

T

T

I

I

G

A

G

P

V

V

I

E

V

L

G

G

N
x
D

N

N

V

S

I

L

I

V

G

G

A

A

G

G

S

S

V

T

V

I

V

T

K

K

D

D

I

V

P

P

D

A

N

D

S

A

V

I

A

A

V

I

G

G

binding acetyl coenzyme *a: Y364 (≠ T134), V383 (vs. gap), N384 (≠ K138), Y385 (≠ A139)
binding calcium ion: D397 (= D151), D415 (≠ N169)

Sites not aligning to the query:

active site: 14
binding calcium ion: 101, 226
binding uridine-diphosphate-n-acetylglucosamine: 7, 8, 9, 10, 71, 74, 76, 77, 137, 138, 153, 168, 169, 196, 198, 226

1hm8A Crystal structure of s.Pneumoniae n-acetylglucosamine-1-phosphate uridyltransferase, glmu, bound to acetyl coenzyme a (see paper)
39% identity, 38% coverage: 116:191/201 of query aligns to 346:437/458 of 1hm8A

query
sites
1hm8A

C

V

E

E

I

V

N

K

A

G

K

S

S

S

I

I

G

G

K

E

N

N

F

T

S

K

C

A

R

G

H

H

L

L

T

T

T

Y

L

I

G

G

N

N

-

C

-

E

-

V

-

G

-

S

-

N

-

V

-

N

-

F

-

G

-

A

-

G

-

T

-

I

-

T

-
x
V

K

N

A
x
Y

D

D

G

G

D

K

N

N

D

K

N

Y

R

K

P

T

V

V

I

I

G

G

D

D

N

N

V

V

T

F

L

V

G

G

V

S

N

N

V

S

T

T

I

I

G

A

G

P

V

V

I

E

V

L

G

G

N

D

N

N

V

S

I

L

I

V

G

G

A

A

G

G

S

S

V

T

V

I

V

T

K

K

D

D

I

V

P

P

D

A

N

D

S

A

V

I

A

A

V

I

G

G

binding acetyl coenzyme *a: V383 (vs. gap), Y385 (≠ A139)

Sites not aligning to the query:

active site: 14

4ac3A S.Pneumoniae glmu in complex with an antibacterial inhibitor (see paper)
39% identity, 38% coverage: 116:191/201 of query aligns to 344:435/456 of 4ac3A

query
sites
4ac3A

C

V

E

E

I

V

N

K

A

G

K

S

S

S

I

I

G

G

K

E

N

N

F

T

S

K

C

A

R

G

H

H

L

L

T

T

T

Y

L

I

G

G

N

N

-

C

-

E

-

V

-

G

-

S

-

N

-

V

-

N

-

F

-

G

-

A

-

G

-

T

-

I

-

T

-
x
V

K

N

A
x
Y

D
|
D

G

G

D

K

N

N

D

K

N

Y

R

K

P

T

V

V

I

I

G

G

D

D

N

N

V

V

T

F

L

V

G

G

V

S

N

N

V

S

T

T

I

I

I
|
I

G

A

G

P

V

V

I

E

V

L

G

G

N

D

N

N

V

S

I

L

I

V

G

G

A

A

G

G

S

S

V

T

V

I

V

T

K

K

D

D

I

V

P

P

D

A

N

D

S

A

V

I

A

A

V

I

G

G

binding N-{2,4-DIMETHOXY-5-[(2-PIPERIDIN-1-YLBENZYL)sulfamoyl]phenyl}acetamide: V381 (vs. gap), Y383 (≠ A139), D384 (= D140), I406 (= I162)

Sites not aligning to the query:

active site: 14

7kr9A Bifunctional enzyme glmu bound to zn(ii) (see paper)
39% identity, 38% coverage: 116:191/201 of query aligns to 341:432/453 of 7kr9A

query
sites
7kr9A

C

V

E

E

I

V

N

K

A

G

K

S

S

S

I

I

G

G

K

E

N

N

F

T

S

K

C

A

R

G

H

H

L

L

T

T

T
x
Y

L

I

G

G

N

N

-

C

-

E

-

V

-

G

-

S

-

N

-

V

-

N

-

F

-

G

-

A

-

G

-

T

-

I

-

T

-
x
V

K
x
N

A
x
Y

D

D

G

G

D

K

N

N

D

K

N

Y

R

K

P

T

V

V

I

I

G

G

D

D

N

N

V

V

T

F

L

V

G

G

V

S

N

N

V

S

T

T

I

I

G

A

G

P

V

V

I

E

V

L

G

G

N

D

N

N

V

S

I

L

I

V

G

G

A

A

G

G

S

S

V

T

V

I

V

T

K

K

D

D

I

V

P

P

D

A

N

D

S

A

V

I

A

A

V

I

G

G

binding acetyl coenzyme *a: Y359 (≠ T134), V378 (vs. gap), N379 (≠ K138), Y380 (≠ A139)

Sites not aligning to the query:

binding zinc ion: 309, 324

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
41% identity, 39% coverage: 123:201/201 of query aligns to 103:185/190 of 5u2kA

query
sites
5u2kA

I

I

G

G

K

P

N

N

-

C

-

G

-

F

F
x
Y

S

T

C
x
A

R

T

H
|
H

L

P

T

L

T

N

L

F

G

H

N

H

K

R

A

N

D

E

G

G

D

F

N

E

D

K

N

A

R

G

P

P

V

I

-

H

I

I

G

G

D

S

N

N

V

T

T

W

L

F

G

G

V

G

N

H

V

V

T

A

I

V

I
x
L

G

P

G

G

V

V

I

T

V

I

G

G

N

E

N

G

V

S

I

V

I

I

G

G

A

A

G

G

S

S

V

V

V
x
T

K
|
K

D

D

I

I

P

P

D

P

N

H

S

S

V

L

A

A

V

V

G

G

N

N

P

P

C

C

R

K

V

V

I

V

K

R

T

K

I

I

E

D

binding coenzyme a: Y110 (≠ F127), A112 (≠ C129), H114 (= H131), L146 (≠ I162), T164 (≠ V180), K165 (= K181)

Sites not aligning to the query:

binding coenzyme a: 90

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
60% identity, 26% coverage: 149:200/201 of query aligns to 133:184/201 of 1krvA

query
sites
1krvA

I

I

G

G

D

N

N

N

V

V

T
x
W

L

I

G
|
G

V
x
S

N

H

V

V

T

V

I

I

I
x
N

G

P

G

G

V

V

I

T

V

I

G

G

N

D

N

N

V

S

I

V

I

I

G

G

A
|
A

G

G

S

S

V

I

V

V

V

T

K

K

D

D

I

I

P

P

D

P

N

N

S

V

V

V

A

A

V

A

G

G

N

V

P
|
P

C

C

R
|
R

V

V

I

I

K
x
R

T

E

I

I

binding coenzyme a: W138 (≠ T154), G140 (= G156), S141 (≠ V157), N146 (≠ I162), A159 (= A175), P177 (= P193), R179 (= R195), R182 (≠ K198)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25, 70, 82, 84, 90, 92, 126
binding coenzyme a: 104, 110, 112

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
60% identity, 26% coverage: 149:200/201 of query aligns to 133:184/201 of 1kruA

query
sites
1kruA

I

I

G

G

D

N

N

N

V

V

T
x
W

L

I

G
|
G

V
x
S

N

H

V

V

T

V

I

I

I

N

G

P

G

G

V

V

I

T

V

I

G

G

N

D

N

N

V

S

I

V

I

I

G
|
G

A
|
A

G

G

S

S

V

I

V

V

V

T

K

K

D

D

I

I

P

P

D

P

N

N

S

V

V

V

A

A

V

A

G

G

N

V

P
|
P

C

C

R

R

V

V

I

I

K
x
R

T

E

I

I

binding coenzyme a: W138 (≠ T154), G140 (= G156), S141 (≠ V157), G158 (= G174), A159 (= A175), P177 (= P193), R182 (≠ K198)

Sites not aligning to the query:

binding coenzyme a: 114
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25, 62, 70, 82, 82, 84, 90, 92, 102, 114, 126

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
60% identity, 26% coverage: 149:200/201 of query aligns to 134:185/203 of P07464

query
sites
P07464

I

I

G

G

D

N

N

N

V

V

T

W

L

I

G

G

V
x
S

N

H

V

V

T

V

I

I

I

N

G

P

G

G

V

V

I

T

V

I

G

G

N

D

N

N

V

S

I

V

I

I

G

G

A
|
A

G

G

S

S

V

I

V

V

V
x
T

K
|
K

D

D

I

I

P

P

D

P

N

N

S

V

V

V

A

A

V

A

G

G

N

V

P

P

C

C

R
|
R

V

V

I

I

K
x
R

T

E

I

I

S142 (≠ V157) binding in other chain
A160 (= A175) binding in other chain
TK 165:166 (≠ VK 180:181) binding
R180 (= R195) binding
R183 (≠ K198) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding
71 binding
85 binding in other chain; binding in other chain
93 binding
115 H→A: Results in an 1800-fold decrease in catalytic activity.

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
60% identity, 26% coverage: 149:200/201 of query aligns to 133:184/200 of 1krrA

query
sites
1krrA

I

I

G

G

D

N

N

N

V

V

T

W

L

I

G
|
G

V
x
S

N

H

V

V

T

V

I

I

I
x
N

G

P

G

G

V

V

I

T

V

I

G

G

N

D

N

N

V

S

I

V

I

I

G
|
G

A
|
A

G

G

S

S

V

I

V

V

V

T

K

K

D

D

I

I

P

P

D

P

N

N

S

V

V

V

A

A

V
x
A

G

G

N

V

P
|
P

C

C

R

R

V

V

I

I

K
x
R

T

E

I

I

binding acetyl coenzyme *a: G140 (= G156), S141 (≠ V157), N146 (≠ I162), G158 (= G174), A159 (= A175), A174 (≠ V190), P177 (= P193), R182 (≠ K198)

Sites not aligning to the query:

binding acetyl coenzyme *a: 84, 103, 104, 105, 112, 114

Q97R46 Bifunctional protein GlmU; EC 2.7.7.23; EC 2.3.1.157 from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) (see 2 papers)
38% identity, 38% coverage: 116:191/201 of query aligns to 347:438/459 of Q97R46

query
sites
Q97R46

C

V

E

E

I

V

N

K

A

G

K

S

S

S

I

I

G

G

K

E

N

N

F

T

S

K

C

A

R

G

H

H

L

L

T

T

T

Y

L

I

G

G

N

N

-

C

-

E

-

V

-

G

-

S

-

N

-

V

-

N

-

F

-

G

-

A

-

G

-

T

-

I

-

T

-

V

K
x
N

A
x
Y

D

D

G

G

D

K

N

N

D

K

N

Y

R

K

P

T

V

V

I

I

G

G

D

N

N

N

V

V

T

F

L

V

G

G

V

S

N

N

V

S

T

T

I

I

G

A

G

P

V

V

I

E

V

L

G

G

N

D

N

N

V

S

I

L

I

V

G

G

A

A

G

G

S

S

V

T

V

I

V

T

K

K

D

D

I

V

P

P

D

A

N

D

S

A

V

I

A

A

V

I

G

G

NY 385:386 (≠ KA 138:139) binding

Sites not aligning to the query:

8:11 binding
22 binding
72 binding
77:78 binding
101:102 binding
102 binding ; binding
139 binding
154 binding
169 binding
227 binding ; binding ; binding

1g97A S.Pneumoniae glmu complexed with udp-n-acetylglucosamine and mg2+ (see paper)
38% identity, 38% coverage: 116:191/201 of query aligns to 346:437/446 of 1g97A

query
sites
1g97A

C

V

E

E

I

V

N

K

A

G

K

S

S

S

I

I

G

G

K

E

N

N

F

T

S

K

C

A

R

G

H

H

L

L

T

T

T

Y

L

I

G

G

N

N

-

C

-

E

-

V

-

G

-

S

-

N

-

V

-

N

-

F

-

G

-

A

-

G

-

T

-

I

-

T

-

V

K

N

A

Y

D

D

G

G

D

K

N

N

D

K

N

Y

R

K

P

T

V

V

I

I

G

G

D

N

N

N

V

V

T

F

L

V

G

G

V

S

N

N

V

S

T

T

I

I

G

A

G

P

V

V

I

E

V

L

G

G

N

D

N

N

V

S

I

L

I

V

G

G

A

A

G

G

S

S

V

T

V

I

V

T

K

K

D

D

I

V

P

P

D

A

N

D

S

A

V

I

A

A

V

I

G

G

Sites not aligning to the query:

active site: 14
binding magnesium ion: 101, 226
binding uridine-diphosphate-n-acetylglucosamine: 7, 9, 10, 71, 74, 76, 77, 137, 138, 153, 168, 169, 196, 198, 226

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
36% identity, 29% coverage: 143:201/201 of query aligns to 81:144/290 of 4mzuB

query
sites
4mzuB

N
|
N

D
|
D

N
x
K

R

Q

P
|
P

-

R

-

S

-

L

-

K

-

T

V

I

I

V

G

K

D

K

N

G

V

A

T

S

L

I

G
|
G

V
x
A

N

N

V

S

T

T

I

I

I

L

G

P

G

G

V

I

I

L

V

I

G

G

N

E

N

N

V

A

I

M

I

V

G
|
G

A
|
A

G

G

S

A

V

V

V

I

V
x
T

K
|
K

D

N

I

V

P

P

D

D

N

N

S

A

V

I

A

V

V
x
I

G

G

N

N

P

P

C

G

R
|
R

V

I

I

T

K

G

T

Y

I

V

E

E

binding coenzyme a: N81 (= N143), D82 (= D144), K83 (≠ N145), P85 (= P147), G99 (= G156), A100 (≠ V157), G117 (= G174), A118 (= A175), T123 (≠ V180), K124 (= K181), I133 (≠ V190), R138 (= R195)

Sites not aligning to the query:

binding coenzyme a: 72, 80, 199
binding magnesium ion: 58, 76
binding thymine: 20, 21, 38, 39
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
35% identity, 29% coverage: 143:201/201 of query aligns to 81:145/294 of 4mzuF

query
sites
4mzuF

N
|
N

D
|
D

N
x
K

R

Q

P

P

-

R

-

S

-

K

-

I

-

K

-

T

V

I

I

V

G

K

D

K

N

G

V

A

T

S

L

I

G
|
G

V
x
A

N

N

V

S

T

T

I

I

I
x
L

G

P

G

G

V

I

I

L

V

I

G

G

N

E

N

N

V

A

I

M

I

V

G
|
G

A
|
A

G

G

S

A

V

V

V

I

V
x
T

K
|
K

D

N

I

V

P

P

D

D

N

N

S

A

V

I

A

V

V
x
I

G

G

N
|
N

P

P

C

G

R
|
R

V

I

I

T

K

G

T

Y

I

V

E

E

binding coenzyme a: N81 (= N143), D82 (= D144), K83 (≠ N145), G100 (= G156), A101 (≠ V157), L106 (≠ I162), G118 (= G174), A119 (= A175), T124 (≠ V180), K125 (= K181), I134 (≠ V190), N136 (= N192), R139 (= R195)

Sites not aligning to the query:

binding thymine: 20, 21, 38
binding thymidine-5'-diphosphate: 189, 203, 205, 270

3igjC Crystal structure of maltose o-acetyltransferase complexed with acetyl coenzyme a from bacillus anthracis
42% identity, 37% coverage: 127:201/201 of query aligns to 112:187/188 of 3igjC

query
sites
3igjC

F
x
Y

S

T

C
x
A

R

T

H
|
H

L

P

T

L

T

H

L

P

G

V

N

E

K

R

A

N

D

S

G

G

D

K

N

E

D

Y

N

G

R

K

P

P

V

V

-

K

I

I

G

G

D

N

N

N

V

V

T

W

L

V

G
|
G

V

G

N

G

V

A

T

I

I

I

I
x
N

G

P

G

G

V

V

I

S

V

I

G

G

N

D

N

N

V

A

I

V

I

I

G
x
A

A
x
S

G

G

S

A

V

V

V
x
T

K

K

D

D

I

V

P

P

D

N

N

N

S

V

V

V

A

V

V

G

G

G

N
|
N

P

P

C

A

R

K

V

V

I

I

K

K

T

T

I

I

E

E

binding acetyl coenzyme *a: Y112 (≠ F127), A114 (≠ C129), H116 (= H131), G142 (= G156), N148 (≠ I162), A160 (≠ G174), S161 (≠ A175), T166 (≠ V180), N178 (= N192)

Sites not aligning to the query:

binding acetyl coenzyme *a: 84, 106

Query Sequence

>349921 FitnessBrowser__Btheta:349921
MNKRKIRDIGRLTFSLLFFGLYIPHIFLYLFKNSLRKSINADLERRKEKNEVRMNKCLMF
LYYIHTDRYFRNLFYHRVGAVVGALIGWWRLGDRSFVISKTTKIGEGAYFAHPFACEINA
KSIGKNFSCRHLTTLGNKADGDNDNRPVIGDNVTLGVNVTIIGGVIVGNNVIIGAGSVVV
KDIPDNSVAVGNPCRVIKTIE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory