SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 349937 FitnessBrowser__Btheta:349937 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9P7B4 NADP-dependent 3-hydroxy acid dehydrogenase; L-allo-threonine dehydrogenase; EC 1.1.1.381 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
46% identity, 100% coverage: 1:255/256 of query aligns to 4:259/259 of Q9P7B4

query
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Q9P7B4

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S42 (= S38) modified: Phosphoserine
T43 (≠ K39) modified: Phosphothreonine

3asvA The closed form of serine dehydrogenase complexed with NADP+ (see paper)
44% identity, 98% coverage: 5:254/256 of query aligns to 2:248/248 of 3asvA

query
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3asvA

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binding nadp nicotinamide-adenine-dinucleotide phosphate: G7 (= G10), T9 (≠ S12), G11 (= G14), F12 (≠ I15), R32 (= R35), R33 (≠ T36), D54 (= D61), V55 (= V62), N81 (= N88), G83 (= G90), I132 (= I139), S134 (= S141), Y147 (= Y154), K151 (= K158), G178 (= G185), V180 (= V187), T183 (= T189), E184 (≠ N190), F185 (= F191)
binding phosphate ion: S134 (= S141), Y147 (= Y154), G178 (= G185), F185 (= F191)

6ixjA The crystal structure of sulfoacetaldehyde reductase from klebsiella oxytoca (see paper)
47% identity, 95% coverage: 3:245/256 of query aligns to 1:242/251 of 6ixjA

query
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binding 2-hydroxyethylsulfonic acid: S138 (= S141), Y145 (= Y148), Y151 (= Y154)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), T10 (≠ S12), S11 (= S13), G12 (= G14), F13 (≠ I15), R33 (= R35), R34 (≠ T36), D57 (= D61), V58 (= V62), N84 (= N88), A85 (= A89), G86 (= G90), T108 (= T112), I136 (= I139), S138 (= S141), Y151 (= Y154), K155 (= K158), P181 (= P184), G182 (= G185), A184 (≠ V187), T186 (= T189), E187 (≠ N190), F188 (= F191)

Q05016 NADP-dependent 3-hydroxy acid dehydrogenase; L-allo-threonine dehydrogenase; EC 1.1.1.-; EC 1.1.1.381 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
43% identity, 95% coverage: 4:246/256 of query aligns to 14:260/267 of Q05016

query
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Q05016

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N102 (= N88) binding
Y168 (= Y154) binding
K172 (= K158) binding

3rkuA Substrate fingerprint and the structure of NADP+ dependent serine dehydrogenase from saccharomyces cerevisiae complexed with NADP+
43% identity, 95% coverage: 4:246/256 of query aligns to 15:261/268 of 3rkuA

query
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T

S

V

L

V

V

R

R

Y

Y

R

R

G

G

D
x
N

K

E

Q

E

A

Q

A

A

D

K

N

N

F

V

Y

Y

K

K

G

D

I

T

R

T

P

P

L

L

T

M

G

A

D

D

I

V

A

A

E

D

T

L

V

I

Y

V

Y

Y

A

A

A

T

S

S

A

R

P

K

A

Q

H

N

I

T

Q

V

I

I

A

A

E

D

V

T

L

L

L

I

M

F

P

P

T

T

Y

N

Q

Q

A

A

T

S

active site: A107 (≠ V92), N128 (= N113), S156 (= S141), Y169 (= Y154), K173 (= K158), N214 (≠ D199)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G21 (= G10), S23 (= S12), G25 (= G14), I26 (= I15), R49 (= R35), R50 (≠ T36), D76 (= D61), I77 (≠ V62), N103 (= N88), A104 (= A89), G105 (= G90), K106 (≠ L91), S156 (= S141), Y169 (= Y154), K173 (= K158), P199 (= P184), G200 (= G185), V202 (= V187), T204 (= T189), E205 (≠ N190), F206 (= F191)

1xg5C Structure of human putative dehydrogenase mgc4172 in complex with nadp
37% identity, 94% coverage: 2:242/256 of query aligns to 9:255/257 of 1xg5C

query
sites
1xg5C

K

R

A

D

K

R

I

L

V

A

F

L

I

V

T

T

G
|
G

A

A

S
|
S

S

G

G
|
G

I
|
I

G

G

E

A

G

A

C

V

A

A

R

R

K

A

F

L

A

V

K

Q

E

Q

G

G

W

L

N

K

L

V

I

V

L

G

N

C

A
|
A

R
|
R

T
|
T

V

V

S

G

K

N

L

I

E

E

L

L

K

A

A

A

E

E

L

C

E

-

K

K

E

S

Y

A

G

G

I

Y

Q

P

V

G

Y

T

V

L

L

I

P

P

F

Y

-

R

-

C

D
|
D

V
x
L

R

S

D

N

R

E

K

E

Q

D

A

I

A

L

A

S

A

M

L

F

E

S

A

A

L

I

P

R

E

S

E

Q

W

H

K

S

S

G

I

V

D

D

V

I

L

C

I

I

N

N

N
|
N

A

A

G
|
G

L

L

V

A

I

-

G

R

V

P

D

D

K

T

E

L

F

L

E

S

G

G

S

S

L

T

D

S

E

G

W

W

D

K

I

D

M

M

I

F

D

N

T

V

N

N

I

V

R

L

G

A

L

L

L

S

A

I

M

C

T

T

R

R

L

E

V

A

V

Y

P

Q

G

S

M

M

V

K

E

E

R

R

G

N

C

V

-

D

G

G

H

H

I

I

N

N

I
|
I

G

N

S
|
S

I

M

A

S

G

G

D

H

A

R

A

V

Y

L

P

P

G

L

G

S

-

V

-

T

S

H

V

F

Y
|
Y

C

S

A

A

T

T

K
|
K

A

Y

A

A

V

V

K

T

A

A

L

L

S

T

D

E

G

G

L

L

R

R

I

Q

D

E

L

L

-

R

-

E

V

A

D

Q

T

T

P

H

L

I

R

R

V

A

T

T

N

C

I

I

K

S

P

P

G
|
G

M

V

V
|
V

E

E

T
|
T

N

Q

F
|
F

T

A

V

F

V
x
K

R

L

Y

H

R

D

G

K

D

D

K

P

Q

E

A

K

A

A

D

A

N

A

F

T

Y

Y

K

E

G

Q

I

M

R

K

P

C

L

L

T

K

G

P

D

E

D

D

I

V

A

A

E

E

T

A

V

V

Y

I

Y

Y

A

V

A

L

S

S

A

T

P

P

A

A

H

H

I

I

Q

Q

I

I

A

G

E

D

V

I

L

Q

L

M

M

R

P

P

T

T

active site: S150 (= S141), Y165 (= Y154), K169 (= K158)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G17 (= G10), S19 (= S12), G21 (= G14), I22 (= I15), A41 (= A34), R42 (= R35), T43 (= T36), D69 (= D61), L70 (≠ V62), N96 (= N88), G98 (= G90), I148 (= I139), S150 (= S141), Y165 (= Y154), K169 (= K158), G198 (= G185), V200 (= V187), T202 (= T189), F204 (= F191), K207 (≠ V194)

A0A1U8QWA2 Glycine betaine reductase ATRR; Nonribosomal peptide synthetase-like protein ATRR; EC 1.2.1.-; EC 1.1.1.- from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
35% identity, 94% coverage: 1:241/256 of query aligns to 1027:1265/1270 of A0A1U8QWA2

query
sites
A0A1U8QWA2

M
x
L

K
x
S

A
x
G

K
|
K

I
x
V

V
x
A

F
x
V

I
x
V

T
|
T

G
|
G

A
|
A

S
|
S

G
|
G

I
|
I

G
|
G

E
x
A

G
x
A

C
x
V

A
|
A

R
x
T

K
x
A

F
x
L

A
|
A

K
x
R

E
|
E

G
|
G

W
x
A

N
x
H

L
x
V

I
x
A

L
|
L

N
x
G

A
|
A

R
|
R

T
x
R

V
x
L

S
x
D

K
x
A

L
|
L

E
|
E

E
x
S

L
|
L

K
|
K

A
x
E

E
x
K

L
|
L

E
x
S

K
x
A

E
x
S

Y

-

G
|
G

I
x
V

Q
x
K

V
|
V

Y
x
V

V
x
T

L
x
C

P
x
K

F
x
T

D
|
D

V
|
V

R
x
T

D
|
D

R
|
R

K
|
K

Q
|
Q

A
x
V

A
x
E

A
x
G

A
x
L

L
x
V

E
x
K

A
|
A

L
x
A

P
x
T

E
|
E

W
x
L

K
x
G

S
x
P

I
x
V

D
|
D

V
x
I

L
|
L

I
x
V

N
x
A

N
x
C

A
|
A

G
|
G

L

-

V
|
V

I
x
M

G
x
Y

V
x
F

D
x
T

K
x
M

E
x
M

F
x
A

E
x
N

G
x
T

S
x
Q

L
x
M

D
|
D

E
|
E

W
|
W

D
x
E

I
x
R

M
x
T

I
x
V

D
|
D

T
x
V

N
|
N

I
x
C

R
x
K

G
|
G

L
x
I

L
|
L

A
x
N

M
x
S

T
x
L

R
x
A

L
x
S

V
x
T

V
|
V

P
|
P

G
|
G

M
|
M

V
x
L

E
x
A

R
|
R

G
|
G

C
x
K

G
|
G

H
|
H

I
x
V

N
x
A

I
|
I

G
x
S

S
|
S

I
x
D

A
|
A

G
|
G

D
x
R

A
x
K

A
x
V

Y
x
F

P
|
P

G
|
G

G
x
L

S
x
G

V
|
V

Y
|
Y

C
x
S

A
|
A

T
x
S

K
|
K

A
x
F

V
|
V

K
x
E

A
|
A

L
x
T

S
x
L

D
x
Q

G
x
A

L
|
L

R
|
R

I
x
L

D
x
E

L
x
T

V
x
A

D
x
G

T
x
Q

P
x
G

L
|
L

R
|
R

V
|
V

T
|
T

N
x
A

I
x
V

K
x
Q

P
|
P

G
|
G

M
x
N

V
x
T

E
x
A

T
|
T

N
x
D

F
x
L

T

-

V

-

V
x
L

R
x
G

Y
x
M

R
x
S

G
x
T

D
|
D

K
x
A

Q
x
E

A
|
A

A
x
I

D
x
K

N
x
K

F
x
Y

-
x
G

-
x
E

Y
x
P

K
x
S

G
|
G

I
x
A

R
x
Q

P
x
I

L
|
L

T
x
D

G
x
P

D
x
E

D
|
D

I
x
V

A
|
A

E
x
N

T
x
S

V
x
I

Y
x
I

Y
|
Y

A
|
A

A
x
L

S
x
R

A
x
Q

P
|
P

A
x
E

H
|
H

I
x
V

Q

A

I

M

A

N

E

E

V

I

L

L

L

I

M

E

P

P

G1036 (= G10) mutation to A: Compromises binding of the cosubstrate NADPH to aldehyde reductase domain R2. Decreases the aldehyde reductase activity by 4,000-fold; when associated with F-1178.
Y1178 (= Y154) mutation to F: Does not substantially affect carboxylic acid reductase activity but results to a 150-fold loss of aldehyde reductase activity and the accumulation of glycine betaine aldehyde intermediate. Further decreases the aldehyde reductase activity by 4,000-fold; when associated with F-1178.

Sites not aligning to the query:

14:418 Adenylation (A) domain
643:937 Carboxylic acid reductase domain R1
812 Y→F: Abolishes overall carboxylic acid reductase activity but does nor affect aldehyde reductase activity.
1026:1256 Aldehyde reductase domain R2

3p19A Improved NADPH-dependent blue fluorescent protein (see paper)
36% identity, 94% coverage: 4:244/256 of query aligns to 3:237/239 of 3p19A

query
sites
3p19A

K

K

I

L

V

V

F

V

I

I

T

T

G
|
G

A

A

S
|
S

G
|
G

I
|
I

G

G

E

E

G

A

C

I

A

A

R

R

K

R

F

F

A

S

K

E

E

E

G

G

W

H

N

P

L

L

I

L

L

L

N

L

A
|
A

R
|
R

T
x
R

V

V

S

E

K

R

L

L

E

K

E

A

L

L

K

N

A

-

E

-

L

L

E

P

K

N

E

T

Y

L

G

C

I

A

Q

Q

V

V

Y

-

V

-

L

-

P

-

F

-

D
|
D

V
|
V

R

T

D

D

R

K

K

Y

Q

T

A

F

A

D

A

T

A

A

L

I

E

T

A

R

L

A

P

E

E

K

E

I

W

Y

K

G

S

P

I

A

D

D

V

A

L

I

I

V

N

N

N
|
N

A
|
A

G
|
G

L

M

V

M

I

L

-

L

-

G

G

Q

V

I

D

D

K

T

E

Q

F

-

E

-

G

-

S

E

L

A

D

N

E

E

W

W

D

Q

I

R

M

M

I

F

D

D

T

V

N

N

I

V

R

L

G

G

L

L

A

N

M

G

T

M

R

Q

L

A

V

V

V

L

P

A

G

P

M

M

V

K

E

A

R

R

G

N

C

C

G

G

H

T

I

I

N

N

I
|
I

G

S

S
|
S

I

I

A

A

G

G

D

K

A

K

A

T

Y

F

P

P

G

D

G

H

S

A

V

A

Y
|
Y

C

C

A

G

T

T

K
|
K

A

F

A

A

V

V

K

H

A

A

L

I

S

S

D

E

G

N

L

V

R

R

I

E

D

E

L

V

V

A

D

A

T

S

P

N

L

V

R

R

V

V

T

M

N

T

I

I

K

A

P
|
P

G

S

M
x
A

V
|
V

E

K

T
|
T

N
x
E

F
x
L

T

-

V

-

V

L

R

S

Y

H

R

T

G

T

D

S

K

Q

Q

Q

A

I

A

K

D

D

N

G

F

Y

-

D

-

A

-

W

-

R

-

V

-

D

Y

M

K

G

G

G

I

V

R

-

P

-

L

L

T

A

G

A

D

D

I

V

A

A

E

R

T

A

V

V

Y

L

Y

F

A

A

A

Y

S

Q

A

Q

P

P

A

Q

H

N

I

V

Q

C

I

I

A

R

E

E

V

I

L

A

L

L

M

A

P

P

T

T

Y

K

Q

Q

active site: S132 (= S141), Y145 (= Y154), K149 (= K158)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G10), S11 (= S12), S12 (= S13), G13 (= G14), I14 (= I15), A33 (= A34), R34 (= R35), R35 (≠ T36), D53 (= D61), V54 (= V62), N80 (= N88), A81 (= A89), G82 (= G90), I130 (= I139), S132 (= S141), Y145 (= Y154), K149 (= K158), P175 (= P184), A177 (≠ M186), V178 (= V187), T180 (= T189), E181 (≠ N190), L182 (≠ F191)

2japA Clavulanic acid dehydrogenase: structural and biochemical analysis of the final step in the biosynthesis of the beta- lactamase inhibitor clavulanic acid (see paper)
39% identity, 95% coverage: 1:244/256 of query aligns to 3:244/245 of 2japA

query
sites
2japA

M

L

K

Q

A

G

K

K

I

V

V

A

F

L

I

I

T

T

G
|
G

A

A

S
|
S

G
|
G

I
|
I

G

G

E

E

G

A

C

T

A

A

R

R

K

A

F

L

A

A

K

A

E

E

G

G

W

A

N

A

L

V

I

A

L

I

N

A

A
|
A

R
|
R

T
x
R

V

V

S

E

K

K

L

L

E

R

E

A

L

L

K

G

A

D

E

E

L

L

E

-

K

T

E

A

Y

A

G

G

I

A

Q

K

V

V

Y

H

V

V

L

L

P

E

F
x
L

D
|
D

V
|
V

R

A

D

D

R

R

K

Q

Q

G

A

V

A

D

A

A

L

V

E

A

A

S

L

T

P

V

E

E

E

A

W

L

K

G

S

G

I

L

D

D

V

I

L

L

I

V

N

N

N
|
N

A
|
A

G
|
G

L

I

V
x
M

I

L

G

L

V

G

D

P

K

V

E

E

F

D

E

A

G

D

S

T

L

T

D

D

E

-

W

W

D

T

I

R

M

M

I

I

D

D

T
|
T

N

N

I

L

R

L

G

G

L

L

L

M

A

Y

M

M

T

T

R

R

L

A

V

A

V

L

P

P

G

H

M

L

V

L

E

-

R

R

G

S

C

K

G

G

H

T

I

V

N

Q

I
x
M

G
x
S

S
|
S

I

I

A
|
A

G

G

D

R

A

V

A

N

Y

V

P

R

G

N

G

A

S

A

V

V

Y
|
Y

C

Q

A

A

T

T

K
|
K

A

F

A

G

V

V

K

N

A

A

L

F

S

S

D

E

G

T

L

L

R

R

I

Q

D

E

L

V

V

T

D

E

T

R

P

G

L

V

R

R

V

V

T

V

N

V

I

I

K

E

P
|
P

G

G

M
x
T

V
x
T

E

D

T
|
T

N
x
E

F
x
L

T

R

-

G

V

H

V

I

R

T

Y

H

R

T

G
x
A

D

T

K

K

Q

E

A

M

A
x
Y

D

E

N

Q

F
x
R

Y

I

K

S

G

Q

I

I

R

R

P

K

L

L

T

Q

G

A

D

Q

D

D

I

I

A

A

E

E

T

A

V

V

Y

R

Y

Y

A

A

A

V

S

T

A

A

P

P

A

H

H

H

I

A

Q

T

I

V

A

H

E

E

V

I

L

F

L

I

M

R

P

P

T

T

Y

D

Q

Q

active site: S140 (= S141), Y153 (= Y154), K157 (= K158), A198 (≠ G198)
binding (2r,3z,5r)-3-(2-hydroxyethylidene)-7-oxo-4-oxa-1-azabicyclo[3.2.0]heptane-2-carboxylic acid: M93 (≠ V92), S140 (= S141), A142 (= A143), Y153 (= Y154), T185 (≠ M186), Y203 (≠ A203), R206 (≠ F206)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G10), S14 (= S12), S15 (= S13), G16 (= G14), I17 (= I15), A36 (= A34), R37 (= R35), R38 (≠ T36), L61 (≠ F60), D62 (= D61), V63 (= V62), N89 (= N88), A90 (= A89), G91 (= G90), T112 (= T112), M138 (≠ I139), S139 (≠ G140), S140 (= S141), Y153 (= Y154), K157 (= K158), P183 (= P184), T185 (≠ M186), T186 (≠ V187), T188 (= T189), E189 (≠ N190), L190 (≠ F191)

2jahC Biochemical and structural analysis of the clavulanic acid dehydeogenase (cad) from streptomyces clavuligerus (see paper)
39% identity, 95% coverage: 1:244/256 of query aligns to 4:245/246 of 2jahC

query
sites
2jahC

M

L

K

Q

A

G

K

K

I

V

V

A

F

L

I

I

T

T

G
|
G

A

A

S
|
S

G
|
G

I
|
I

G

G

E

E

G

A

C

T

A

A

R

R

K

A

F

L

A

A

K

A

E

E

G

G

W

A

N

A

L

V

I

A

L

I

N

A

A
|
A

R
|
R

T
x
R

V

V

S

E

K

K

L

L

E

R

E

A

L

L

K

G

A

D

E

E

L

L

E

T

K

A

E

A

Y

-

G

G

I

A

Q

K

V

V

Y

H

V

V

L

L

P

E

F

L

D
|
D

V
|
V

R

A

D

D

R

R

K

Q

Q

G

A

V

A

D

A

A

L

V

E

A

A

S

L

T

P

V

E

E

E

A

W

L

K

G

S

G

I

L

D

D

V

I

L

L

I

V

N

N

N
|
N

A
|
A

G
|
G

L

I

V

M

I

L

G

L

V

G

D

P

K

V

E

E

F

D

E

A

G

D

S

T

L

T

D

D

E

-

W

W

D

T

I

R

M

M

I

I

D

D

T
|
T

N

N

I

L

R

L

G

G

L

L

L

M

A

Y

M

M

T

T

R

R

L

A

V

A

V

L

P

P

G

H

M

L

V

L

E

-

R

R

G

S

C

K

G

G

H

T

I

V

N

Q

I
x
M

G

S

S
|
S

I

I

A

A

G

G

D

R

A

V

A

N

Y

V

P

R

G

N

G

A

S

A

V

V

Y
|
Y

C

Q

A

A

T

T

K
|
K

A

F

A

G

V

V

K

N

A

A

L

F

S

S

D

E

G

T

L

L

R

R

I

Q

D

E

L

V

V

T

D

E

T

R

P

G

L

V

R

R

V

V

T

V

N

V

I

I

K

E

P
|
P

G
|
G

M
x
T

V
x
T

E

D

T
|
T

N
x
E

F
x
L

T

R

-

G

V

H

V

I

R

T

Y

H

R

T

G
x
A

D

T

K

K

Q

E

A

M

A

Y

D

E

N

Q

F

R

Y

I

K

S

G

Q

I

I

R

R

P

K

L

L

T

Q

G

A

D

Q

D

D

I

I

A

A

E

E

T

A

V

V

Y

R

Y

Y

A

A

A

V

S

T

A

A

P

P

A

H

H

H

I

A

Q

T

I

V

A

H

E

E

V

I

L

F

L

I

M

R

P

P

T

T

Y

D

Q

Q

active site: S141 (= S141), Y154 (= Y154), K158 (= K158), A199 (≠ G198)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G13 (= G10), S15 (= S12), S16 (= S13), G17 (= G14), I18 (= I15), A37 (= A34), R38 (= R35), R39 (≠ T36), D63 (= D61), V64 (= V62), N90 (= N88), A91 (= A89), G92 (= G90), T113 (= T112), M139 (≠ I139), S141 (= S141), Y154 (= Y154), K158 (= K158), P184 (= P184), G185 (= G185), T186 (≠ M186), T187 (≠ V187), T189 (= T189), E190 (≠ N190), L191 (≠ F191)

2ehdB Crystal structure analysis of oxidoreductase
35% identity, 93% coverage: 6:242/256 of query aligns to 7:213/213 of 2ehdB

query
sites
2ehdB

V

V

F

L

I

I

T

T

G

G

A

A

S

S

S

R

G

G

I

I

G

G

E

E

G

A

C

T

A

A

R

R

K

L

F

L

A

H

K

A

E

K

G

G

W

Y

N

R

L

V

I

G

L

L

N

M

A

A

R
|
R

T
x
D

V

E

S

K

K

R

L

L

E

Q

E

A

L

L

K

A

A

A

E

E

L

L

E

E

K

G

E

-

Y

-

G

-

I

-

Q

-

V

A

Y

L

V

P

L

L

P

P

F

G

D

D

V

V

R

R

D

E

R

E

K

G

Q

D

A

W

A

A

A

R

A

A

L

V

E

A

A

A

L

M

P

E

E

E

E

A

W

F

K

G

S

E

I

L

D

S

V

A

L

L

I

V

N

N

A

A

G

G

L

-

V

-

I

V

G

G

V

V

D

M

K

K

E

P

-

V

F

H

E

E

G

L

S

T

L

L

D

E

E

E

W

W

D

R

I

L

M

V

I

L

D

D

T

T

N

N

I

L

R

T

G

G

L

A

L

F

A

L

M

G

T

I

R

R

L

H

V

A

V

V

P

P

G

A

M

L

V

L

E

R

R

R

G

G

C

G

G

G

H

T

I

I

I

V

N

N

I

V

G
|
G

S

S

I

L

A

A

G

G

D

K

A

N

A

P

Y

F

P

K

G

G

S

A

V

A

Y
|
Y

C

N

A

A

T

S

K
|
K

A

F

A

G

V

L

K

L

A

G

L

L

S

A

D

G

G

A

L

A

R

M

I

L

D

D

L

L

V

R

D

E

T

A

P

N

L

V

R

R

V

V

T

V

N

N

I

V

K

L

P

P

G

G

M

S

V

V

E

K

T

-

N

-

F

-

T

-

V

-

R

-

Y

-

R

-

G

-

D

-

K

-

Q

-

A

-

D

-

N

-

F

-

Y

-

K

-

G

-

I

L

R

K

P

P

L

-

T

-

G

-

D

E

D

D

I

V

A

A

E

Q

T

A

V

V

Y

L

Y

F

A

A

A

L

S

E

A

M

P

P

A

G

H

H

I

A

Q

M

I

V

A

S

E

E

V

I

L

E

L

L

M

R

P

P

T

T

active site: G135 (= G140), Y149 (= Y154), K153 (= K158)
binding cobalt (ii) ion: R36 (= R35), D37 (≠ T36)

2cfcA Structural basis for stereo selectivity in the (r)- and (s)-hydroxypropylethane thiosulfonate dehydrogenases (see paper)
32% identity, 77% coverage: 3:200/256 of query aligns to 2:199/250 of 2cfcA

query
sites
2cfcA

A

S

K

R

I

V

V

A

F

I

I

V

T

T

G
|
G

A

A

S

S

S
|
S

G
|
G

I
x
N

G

G

E

L

G

A

C

I

A

A

R

T

K

R

F

F

A

L

K

A

E

R

G

G

W

D

N

R

L

V

I

A

L

A

N

L

A
x
D

R
x
L

T

S

V

A

S

E

K

T

L

L

E

E

L

T

K

A

A

R

E

T

L

H

E

W

K

H

E

A

Y

Y

G

A

I

D

Q

K

V

V

Y

L

V

R

L

V

P

R

F
x
A

D
|
D

V
|
V

R

A

D

D

R

E

K

G

Q

D

A

V

A

N

A

A

L

I

E

A

A

A

L

T

P

M

E

E

E

Q

W

F

K

G

S

A

I

I

D

D

V

V

L

L

I

V

N

N

N
|
N

A
|
A

G
|
G

L

I

V

T

I

G

G

N

V

S

D

E

K

A

E

G

F

V

E

L

G

H

S

T

-

T

-

P

L

V

D

E

E

Q

W

F

D

D

I

K

M

V

I

M

D

A

T

V

N

N

I

V

R

R

G

G

L

I

L

F

A

L

M

G

T

C

R

R

L

A

V

V

V

L

P

P

G

H

M

M

V

L

E

L

R

Q

G

G

C

A

G

G

H

V

I

I

I

V

N

N

I
|
I

G

A

S
|
S

I

V

A

A

G

S

D

L

A

V

A

A

Y
x
F

P

P

G

G

G
x
R

S

S

V

A

Y
|
Y

C

T

A

T

T

S

K
|
K

A

G

A

A

V

V

K

L

A

Q

L

L

S

T

D

K

G

S

L

V

R

A

I

V

D

D

L

Y

V

A

D

G

T

S

P

G

L

I

R

R

V

C

T

N

N

A

I

V

K

C

P
|
P

G
|
G

M
|
M

V
x
I

E

E

T
|
T

N
x
P

F
x
M

T
|
T

V

-

R

Q

Y
x
W

R
|
R

G

L

D

D

K

Q

active site: G13 (= G14), S142 (= S141), Y155 (= Y154), K159 (= K158)
binding (2-[2-ketopropylthio]ethanesulfonate: F149 (≠ Y148), R152 (≠ G151), Y155 (= Y154), W195 (≠ Y196), R196 (= R197)
binding nicotinamide-adenine-dinucleotide: G9 (= G10), S12 (= S13), G13 (= G14), N14 (≠ I15), D33 (≠ A34), L34 (≠ R35), A59 (≠ F60), D60 (= D61), V61 (= V62), N87 (= N88), A88 (= A89), G89 (= G90), I140 (= I139), P185 (= P184), G186 (= G185), M187 (= M186), I188 (≠ V187), T190 (= T189), P191 (≠ N190), M192 (≠ F191), T193 (= T192)

Q56840 2-(R)-hydroxypropyl-CoM dehydrogenase; R-HPCDH; 2-[(R)-2-hydroxypropylthio]ethanesulfonate dehydrogenase; Aliphatic epoxide carboxylation component III; Epoxide carboxylase component III; RHPCDH1; EC 1.1.1.268 from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) (see 4 papers)
32% identity, 77% coverage: 3:200/256 of query aligns to 2:199/250 of Q56840

query
sites
Q56840

A

S

K

R

I

V

V

A

F

I

I

V

T

T

G

G

A

A

S

S

S
|
S

G
|
G

I
x
N

G

G

E

L

G

A

C

I

A

A

R

T

K

R

F

F

A

L

K

A

E

R

G

G

W

D

N

R

L

V

I

A

L

A

N

L

A
x
D

R

L

T

S

V

A

S

E

K

T

L

L

E

E

L

T

K

A

A

R

E

T

L

H

E

W

K

H

E

A

Y

Y

G

A

I

D

Q

K

V

V

Y

L

V

R

L

V

P

R

F

A

D
|
D

V
|
V

R

A

D

D

R

E

K

G

Q

D

A

V

A

N

A

A

L

I

E

A

A

A

L

T

P

M

E

E

E

Q

W

F

K

G

S

A

I

I

D

D

V

V

L

L

I

V

N

N

N
|
N

A

A

G

G

L

I

V

T

I

G

G

N

V

S

D

E

K

A

E

G

F

V

E

L

G

H

S

T

-

T

-

P

L

V

D

E

E

Q

W

F

D

D

I

K

M

V

I

M

D

A

T

V

N

N

I

V

R

R

G

G

L

I

L

F

A

L

M

G

T

C

R

R

L

A

V

V

V

L

P

P

G

H

M

M

V

L

E

L

R

Q

G

G

C

A

G

G

H

V

I

I

I

V

N

N

I

I

G

A

S
|
S

I

V

A

A

G

S

D

L

A

V

A

A

Y

F

P

P

G

G

G
x
R

S

S

V

A

Y
|
Y

C

T

A

T

T

S

K
|
K

A

G

A

A

V

V

K

L

A

Q

L

L

S

T

D

K

G

S

L

V

R

A

I

V

D

D

L

Y

V

A

D

G

T

S

P

G

L

I

R
|
R

V

C

T

N

N

A

I

V

K

C

P

P

G

G

M

M

V
x
I

E
|
E

T
|
T

N
x
P

F
x
M

T

T

V

-

R

Q

Y
x
W

R
|
R

G

L

D

D

K

Q

SGN 12:14 (≠ SGI 13:15) binding
D33 (≠ A34) binding
DV 60:61 (= DV 61:62) binding
N87 (= N88) binding
S142 (= S141) mutation to A: Retains weak activity. 120-fold decrease in kcat.; mutation to C: Loss of activity.
R152 (≠ G151) binding ; mutation to A: Almost loss of activity with the natural substrate 2-KPC, but does not affect activity with 2-butanone as substrate.
Y155 (= Y154) mutation Y->E,F: Loss of activity.
K159 (= K158) mutation to A: Loss of activity.
R179 (= R178) mutation to A: Loss of activity.
IETPM 188:192 (≠ VETNF 187:191) binding
WR 195:196 (≠ YR 196:197) binding
R196 (= R197) mutation to A: Almost loss of activity with the natural substrate 2-KPC, but does not affect activity with 2-butanone as substrate.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
203 R→A: Slight decrease in catalytic efficiency.
209 R→A: Does not affect catalytic efficiency.

4qecA Elxo with NADP bound (see paper)
27% identity, 92% coverage: 4:238/256 of query aligns to 3:239/248 of 4qecA

query
sites
4qecA

K

K

I

N

V

V

F

L

I

I

T

T

G

G

A

G

S

F

S
x
K

G
|
G

I
|
I

G

G

E

K

G

Q

C

V

A

A

R

L

K

E

F

F

A

L

K

K

E

N

G

D

W

Y

N

H

L

V

I

C

L

I

N

T

A
x
S

R
|
R

T

Y

V

F

S

E

K
|
K

L

-

E

E

E

K

L

R

K

I

A

P

E

H

L

L

E

F

K

S

E

S

Y

Y

G

E

I

E

Q

N

V

I

Y

S

V

F

L

Y

P

Q

F

L

D
|
D

V
|
V

R

T

D

D

R

E

K

E

Q

Q

A

V

A

N

A

E

A

I

L

I

E

N

A

K

L

I

P

V

E

K

W

F

K

G

S

R

I

L

D

D

V

V

L

L

I

V

N

N

N
|
N

A
|
A

G
|
G

L

I

V

S

I

L

G

S

V

D

D

G

K

L

E

L

F

T

E

E

G

T

S

K

L

T

D

T

E

D

W

F

D

N

I

K

M

M

I

I

D

N

T

T

N
|
N

I

I

R

L

G

G

L

T

L

Y

A

F

M

C

T

M

R

K

L

Y

V

A

V

L

P

K

G

H

M

M

V

Q

E

K

R

V

G

S

C

C

G

G

H

A

I

I

I

V

N

N

I
|
I

G

S

S
|
S

I

I

A

T

G

G

D

L

A

S

A

G

Y

F

P

P

G

Y

G

S

S

I

V

L

Y
|
Y

C

G

A

S

T

T

K
|
K

A

H

A

A

V

V

K

I

A

G

L

L

S

T

D

K

G

G

L

A

R

A

I

V

D

E

L

F

V

A

D

D

T

K

P

G

L

I

R

K

V

I

T

N

N

A

I

V

K

A

P
|
P

G

G

M

I

V
x
I

E

K

T

T

N

E

F

-

T

T

V

L

V

Q

R

K

Y

E

R

I

G

D

D

S

K

G

Q

E

A

F

A

S

D

E

N

D

F

S

Y

I

K

S

G

S

I

I

R

H

P

P

L

M

-

Q

-

K

-

L

-

G

T

T

G

T

D

L

D

D

I

V

A

A

E

K

T

G

V

I

Y

Y

Y

F

A

L

A

A

S

N

A

E

P

D

A

N

H

N

I

F

Q

I

I

T

A

G

E

H

V

V

L

L

active site: G13 (= G14), N111 (= N113), S139 (= S141), Y152 (= Y154), K156 (= K158)
binding nadp nicotinamide-adenine-dinucleotide phosphate: K12 (≠ S13), G13 (= G14), I14 (= I15), S33 (≠ A34), R34 (= R35), K38 (= K39), D59 (= D61), V60 (= V62), N86 (= N88), A87 (= A89), G88 (= G90), I137 (= I139), Y152 (= Y154), K156 (= K158), P182 (= P184), I185 (≠ V187)

4bmvC Short-chain dehydrogenase from sphingobium yanoikuyae in complex with NADPH
39% identity, 73% coverage: 6:191/256 of query aligns to 6:189/258 of 4bmvC

query
sites
4bmvC

V

V

F

L

I

I

T

T

G
|
G

A

A

S
|
S

G
|
G

I
|
I

G

G

E

A

G

T

C

Y

A

A

R

E

K

R

F

F

A

A

K

R

E

R

G

G

W

H

N

D

L

L

I

V

L

L

N

V

A

A

R
|
R

T
x
D

V

K

S

V

K

R

L

L

E

D

E

A

L

L

K

A

A

A

E

R

L

L

E

R

K

D

E

E

Y

S

G

G

I

V

Q

A

V

V

Y

E

V

A

L

L

P

Q

F
x
A

D
|
D

V

L

R

-

D

T

R

R

K

P

Q

A

A

D

A

L

A

A

L

V

E

E

A

I

L

R

P

L

E

R

E

E

W

D

K

A

S

R

I

I

D

G

V

I

L

L

I

I

N

N

N
|
N

A

A

G
|
G

L

M

V

A

I

Q

G

S

-

G

-

F

V

V

D

Q

K

Q

E

T

F

A

E

E

G

G

S

-

L

-

D

-

E

-

W

I

D

E

I

R

M

L

I

I

D

T

T
x
L

N
|
N

I

T

R

T

G

A

L

L

L

T

A

R

M

L

T

A

R

A

L

A

V

V

V

A

P

P

G

R

M

F

V

V

E

Q

R

S

G

G

C

T

G

G

H

A

I

I

I

V

N

N

I
|
I

G

G

S
|
S

I

V

A

V

G

G

D

F

A

A

A

P

Y

E

P

F

G

G

G

M

S

S

V

I

Y
|
Y

C

G

A

A

T

T

K
|
K

A

A

A

F

V

V

K

L

A

F

L

L

S

S

D

Q

G

G

L

L

R

N

I

L

D

E

L

L

V

S

D

P

T

S

P

G

L

I

R

Y

V

V

T

Q

N

A

I

V

K

L

P
|
P

G

A

M

A

V
x
T

E

R

T
|
T

N
x
E

F
x
I

active site: N111 (= N113), S139 (= S141), Y152 (= Y154), K156 (= K158)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G10), S12 (= S12), S13 (= S13), G14 (= G14), I15 (= I15), R35 (= R35), D36 (≠ T36), A60 (≠ F60), D61 (= D61), N87 (= N88), G89 (= G90), L110 (≠ T112), I137 (= I139), S139 (= S141), Y152 (= Y154), K156 (= K158), P182 (= P184), T185 (≠ V187), T187 (= T189), E188 (≠ N190), I189 (≠ F191)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 190

3ai2A The crystal structure of l-sorbose reductase from gluconobacter frateurii complexed with NADPH (see paper)
33% identity, 74% coverage: 1:189/256 of query aligns to 5:192/263 of 3ai2A

query
sites
3ai2A

M

I

K

S

A

G

K

K

I

V

V

A

F

V

I

I

T

T

G
|
G

A

S

S
|
S

G
|
G

I
|
I

G

G

E

L

G

A

C

I

A

A

R

E

K

G

F

F

A

A

K

K

E

E

G

G

W

A

N

H

L

I

I

V

L

L

N

V

A
|
A

R
|
R

T
x
Q

V

V

S

D

K

R

L

L

E

H

E

E

L

A

K

A

A

R

E

S

L

L

E

K

K

E

E

K

Y

F

G

G

I

V

Q

R

V

V

Y

L

V

E

L

V

P

A

F
x
V

D
|
D

V
|
V

R

A

D

T

R

P

K

E

Q

G

A

V

A

D

A

A

A

V

L

V

E

E

A

S

L

V

P

R

E

S

W

F

K

G

S

G

I

A

D

D

V

I

L

L

I

V

N

N

N
|
N

A

A

G
|
G

L

-

V

-

I

T

G

G

V

S

D

N

K

E

E

T

F

I

E

M

G

E

S

A

L

A

D

D

E

E

-

K

W

W

D

Q

I

F

M

Y

I

W

D

E

T

L

N

H

I

V

R

M

G

A

L

A

L

V

A

R

M

L

T

A

R

R

L

G

V

L

V

V

P

P

G

G

M

M

V

R

E

A

R

R

G

G

C

G

G

G

H

A

I

I

N

H

I
x
N

G

A

S
|
S

I

I

A

C

G

A

D

V

A

Q

A

P

Y

L

P

W

G

Y

G

E

S

P

V

I

Y
|
Y

C

N

A

V

T

T

K
|
K

A

A

V

L

K

M

A

M

L

F

S

S

D

K

G

T

L

L

R

A

I

T

D

E

L

V

V

I

D

K

T

D

P

N

L

I

R

R

V

V

T

N

N

C

I

I

K

N

P
|
P

G

G

M

L

V
x
I

E

L

T
|
T

active site: G18 (= G14), S144 (= S141), Y157 (= Y154), K161 (= K158)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G14 (= G10), S16 (= S12), S17 (= S13), G18 (= G14), I19 (= I15), A38 (= A34), R39 (= R35), Q40 (≠ T36), V64 (≠ F60), D65 (= D61), V66 (= V62), N92 (= N88), G94 (= G90), N142 (≠ I139), Y157 (= Y154), K161 (= K158), P187 (= P184), I190 (≠ V187), T192 (= T189)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 195

3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
31% identity, 74% coverage: 4:192/256 of query aligns to 2:189/239 of 3sj7A

query
sites
3sj7A

K

K

I

S

V

A

F

L

I

V

T

T

G
|
G

A

A

S
|
S

S
x
R

G
|
G

I
|
I

G

G

E

R

G

S

C

I

A

A

R

L

K

Q

F

L

A

A

K

E

E

E

G

G

W

Y

N

N

L

V

I

A

L

V

N
|
N

A
x
Y

R
x
A

T
x
G

V
x
S

S

K

K

E

L

K

E

A

E

E

L

A

K

V

A

V

E

E

L

E

E

I

K

K

E

A

Y

K

G

G

I

V

Q

D

V

S

Y

F

V

A

L

I

P

Q

F
x
A

D
x
N

V
|
V

R

A

D

D

R

A

K

D

Q

E

A

V

A

K

A

A

A

M

L

I

E

K

A

E

L

V

P

V

E

S

E

Q

W

F

K

G

S

S

I

L

D

D

V

V

L

L

I

V

N

N

N
|
N

A
|
A

G

G

L

I

V

T

I

R

G

D

V

-

D

N

K

L

E

L

F

M

E

R

G

M

S

K

L

E

D

Q

E

E

W

W

D

D

I

D

M

V

I

I

D

D

T
|
T

N

N

I

L

R

K

G

G

L

V

L

F

A

N

M

C

T

I

R

Q

L

K

V

A

V

T

P

P

G

Q

M

M

V

L

E

R

R

Q

G

R

C

S

G

G

H

A

I

I

N

N

I

L

G

S

S
|
S

I

V

A

V

G

G

D

A

A

V

A

G

Y

N

P

P

G

G

G
x
Q

S

A

V

N

Y
|
Y

C

V

A

A

T

T

K
|
K

A

A

A

G

V

V

K

I

A

G

L

L

S

T

D

K

G

S

L

A

R

A

I

R

D

E

L

L

V

A

D

S

T

R

P

G

L

I

R

T

V

V

T

N

N

A

I

V

K

A

P
|
P

G
|
G

M

F

V

I

E

V

T

S

N

D

F

M

T

T

active site: G12 (= G14), S138 (= S141), Q148 (≠ G151), Y151 (= Y154), K155 (= K158)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), S10 (= S12), R11 (≠ S13), I13 (= I15), N31 (= N33), Y32 (≠ A34), A33 (≠ R35), G34 (≠ T36), S35 (≠ V37), A58 (≠ F60), N59 (≠ D61), V60 (= V62), N86 (= N88), A87 (= A89), T109 (= T112), S138 (= S141), Y151 (= Y154), K155 (= K158), P181 (= P184), G182 (= G185)

3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
32% identity, 74% coverage: 4:192/256 of query aligns to 5:192/246 of 3osuA

query
sites
3osuA

K

K

I

S

V

A

F

L

I

V

T

T

G

G

A

A

S

S

S

R

G
|
G

I

I

G

G

E

R

G

S

C

I

A

A

R

L

K

Q

F

L

A

A

K

E

E

E

G

G

W

Y

N

N

L

V

I

A

L

V

N

N

-

Y

A

A

R

G

T

S

V

K

S

E

K

K

L

A

E

E

E

A

L

V

K

V

A

E

E

E

L

I

E

-

K

K

E

A

Y

K

G

G

I

V

Q

D

V

S

Y

F

V

A

L

I

P

Q

F

A

D

N

V

V

R

A

D

D

R

A

K

D

Q

E

A

V

A

K

A

A

A

M

L

I

E

K

A

E

L

V

P

V

E

S

E

Q

W

F

K

G

S

S

I

L

D

D

V

V

L

L

I

V

N

N

N
|
N

A

A

G

G

L

I

V

T

I

R

G

D

V

-

D

N

K

L

E

L

F

M

E

R

G

M

S

K

L

E

D

Q

E

E

W

W

D

D

I

D

M

V

I

I

D

D

T

T

N

N

I

L

R

K

G

G

L

V

L

F

A

N

M

C

T

I

R

Q

L

K

V

A

V

T

P

P

G

Q

M

M

V

L

E

R

R

Q

G

R

C

S

G

G

H

A

I

I

N

N

I

L

G

S

S
|
S

I

V

A

V

G

G

D

A

A

V

A

G

Y

N

P

P

G

G

G
x
Q

S

A

V

N

Y
|
Y

C

V

A

A

T

T

K
|
K

A

A

A

G

V

V

K

I

A

G

L

L

S

T

D

K

G

S

L

A

R

A

I

R

D

E

L

L

V

A

D

S

T

R

P

G

L

I

R

T

V

V

T

N

N

A

I

V

K

A

P

P

G

G

M

F

V

I

E

V

T

S

N

D

F

M

T

T

active site: G15 (= G14), S141 (= S141), Q151 (≠ G151), Y154 (= Y154), K158 (= K158)
binding magnesium ion: N89 (= N88), K158 (= K158)

3ai3C The crystal structure of l-sorbose reductase from gluconobacter frateurii complexed with NADPH and l-sorbose (see paper)
33% identity, 74% coverage: 1:189/256 of query aligns to 5:192/263 of 3ai3C

query
sites
3ai3C

M

I

K

S

A

G

K

K

I

V

V

A

F

V

I

I

T

T

G
|
G

A

S

S
|
S

G
|
G

I
|
I

G

G

E

L

G

A

C

I

A

A

R

E

K

G

F

F

A

A

K

K

E

E

G

G

W

A

N

H

L

I

I

V

L

L

N

V

A
|
A

R
|
R

T
x
Q

V

V

S

D

K
x
R

L

L

E

H

E

E

L

A

K

A

A

R

E

S

L

L

E

K

K

E

E

K

Y

F

G

G

I

V

Q

R

V

V

Y

L

V

E

L

V

P

A

F

V

D
|
D

V
|
V

R

A

D

T

R

P

K

E

Q

G

A

V

A

D

A

A

A

V

L

V

E

E

A

S

L

V

P

R

E

S

W

F

K

G

S

G

I

A

D

D

V

I

L

L

I

V

N

N

N
|
N

A

A

G
|
G

L

-

V

-

I

T

G
|
G

V

S

D

N

K

E

E

T

F

I

E

M

G

E

S

A

L

A

D

D

E

E

-

K

-

W

-

Q

-

F

-

Y

W

W

D

E

I

L

M

L

I

V

D

M

T

A

N

A

I

V

R

R

G

-

L

-

A

-

M

L

T

A

R

R

L

G

V

L

V

V

P

P

G

G

M

M

V

R

E

A

R

R

G

G

C

G

G

G

H

A

I

I

N

H

I
x
N

G

A

S
|
S

I

I

A

C

G

A

D

V

A

Q

A

P

Y

L

P

W

G

Y

G
x
E

S

P

V

I

Y
|
Y

C

N

A

V

T

T

K
|
K

A

A

V

L

K

M

A

M

L

F

S

S

D

K

G

T

L

L

R

A

I

T

D

E

L

V

V

I

D

K

T

D

P

N

L

I

R

R

V

V

T

N

N

C

I

I

K

N

P
|
P

G
|
G

M

L

V
x
I

E

L

T
|
T

active site: G18 (= G14), S144 (= S141), Y157 (= Y154), K161 (= K158)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G14 (= G10), S16 (= S12), S17 (= S13), G18 (= G14), I19 (= I15), A38 (= A34), R39 (= R35), Q40 (≠ T36), R43 (≠ K39), D65 (= D61), V66 (= V62), N92 (= N88), G94 (= G90), N142 (≠ I139), Y157 (= Y154), K161 (= K158), P187 (= P184), G188 (= G185), I190 (≠ V187), T192 (= T189)
binding L-sorbose: G96 (= G94), E154 (≠ G151), Y157 (= Y154)

Sites not aligning to the query:

3ai3A The crystal structure of l-sorbose reductase from gluconobacter frateurii complexed with NADPH and l-sorbose (see paper)
33% identity, 74% coverage: 1:189/256 of query aligns to 5:192/263 of 3ai3A

query
sites
3ai3A

M

I

K

S

A

G

K

K

I

V

V

A

F

V

I

I

T

T

G
|
G

A

S

S
|
S

G
|
G

I
|
I

G

G

E

L

G

A

C

I

A

A

R

E

K

G

F

F

A

A

K

K

E

E

G

G

W

A

N

H

L

I

I

V

L

L

N

V

A
|
A

R
|
R

T
x
Q

V

V

S

D

K
x
R

L

L

E

H

E

E

L

A

K

A

A

R

E

S

L

L

E

K

K

E

E

K

Y

F

G

G

I

V

Q

R

V

V

Y

L

V

E

L

V

P

A

F
x
V

D
|
D

V
|
V

R

A

D

T

R

P

K

E

Q

G

A

V

A

D

A

A

A

V

L

V

E

E

A

S

L

V

P

R

E

S

W

F

K

G

S

G

I

A

D

D

V

I

L

L

I

V

N

N

N
|
N

A

A

G
|
G

L

-

V

-

I

T

G
|
G

V

S

D

N

K

E

E

T

F

I

E

M

G

E

S

A

L

A

D

D

E

E

-

K

-

W

-

Q

-

F

-

Y

W

W

D

E

I

L

M

L

I

V

D

M

T

A

N

A

I

V

R

R

G

-

L

-

A

-

M

L

T

A

R

R

L

G

V

L

V

V

P

P

G

G

M

M

V

R

E

A

R

R

G

G

C

G

G

G

H

A

I

I

N

H

I
x
N

G

A

S
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S

I

I

A

C

G

A

D

V

A

Q

A

P

Y
x
L

P

W

G

Y

G
x
E

S

P

V

I

Y
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Y

C

N

A

V

T

T

K
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K

A

A

V

L

K

M

A

M

L

F

S

S

D

K

G

T

L

L

R

A

I

T

D

E

L

V

V

I

D

K

T

D

P

N

L

I

R

R

V

V

T

N

N

C

I

I

K

N

P
|
P

G
|
G

M

L

V
x
I

E

L

T
|
T

active site: G18 (= G14), S144 (= S141), Y157 (= Y154), K161 (= K158)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G14 (= G10), S16 (= S12), S17 (= S13), G18 (= G14), I19 (= I15), A38 (= A34), R39 (= R35), Q40 (≠ T36), R43 (≠ K39), V64 (≠ F60), D65 (= D61), V66 (= V62), N92 (= N88), G94 (= G90), N142 (≠ I139), S144 (= S141), Y157 (= Y154), K161 (= K158), P187 (= P184), G188 (= G185), I190 (≠ V187), T192 (= T189)
binding L-sorbose: G96 (= G94), S144 (= S141), L151 (≠ Y148), E154 (≠ G151), Y157 (= Y154), G188 (= G185)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 195

Query Sequence

>349937 FitnessBrowser__Btheta:349937
MKAKIVFITGASSGIGEGCARKFAKEGWNLILNARTVSKLEELKAELEKEYGIQVYVLPF
DVRDRKQAAAALEALPEEWKSIDVLINNAGLVIGVDKEFEGSLDEWDIMIDTNIRGLLAM
TRLVVPGMVERGCGHIINIGSIAGDAAYPGGSVYCATKAAVKALSDGLRIDLVDTPLRVT
NIKPGMVETNFTVVRYRGDKQAADNFYKGIRPLTGDDIAETVYYAASAPAHIQIAEVLLM
PTYQATGTISYKKKAE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory