SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 349991 FitnessBrowser__Btheta:349991 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4ho9A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with udp-galactose and utp
68% identity, 98% coverage: 1:288/295 of query aligns to 1:285/294 of 4ho9A

query
sites
4ho9A

M

M

K

K

G

G

I

I

V

I

L
|
L

A

S

G
|
G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

I

L

T

T

K

K

G

V

V

V

S

S

K

K

Q

Q

L

L

P

P

I

V

F

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

I

L

S

S

V

V

L

L

M

M

L
|
L

A

A

G

G

I

I

R

K

E

D

I

I

L

L

I

I

S

S

T

T

P

P

Y

E

D

D

L

T

P

P

G

R

F

F

K

E

R

Q

L

L

G

G

D

G

G

G

S

S

D

E

Y

L

G

G

V

I

H

S

F

L

E

S

Y

Y

A

A

E

V

Q
|
Q

P

S

S

S

P
|
P

D

D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

N

D

D

D

S

N

V

V

C

A

L

L

V

V

L
|
L

G

G

D
|
D

N

N

I

I

F

F

Y

Y

G
|
G

Q
x
H

G
|
G

F

F

T
|
T

H

E

M

L

L

L

K

Q

E

R

A

A

V

-

H

-

T

-

A

A

E

N

E

R

E

K

N

S

K

G

A

A

T

T

V

I

F

F

G

G

Y

Y

W

N

V

V

A

K

D

D

P

P

E

Q

R

R

Y

F

G
|
G

V

V

A

V

E

E

F

F

D

D

K

E

I

K

G

G

N

K

V

V

L

I

S

S

I

I

E

E

E
|
E

K
|
K

P

P

I

E

Q

E

P

P

K

K

S

S

N

S

Y

Y

A

A

V
|
V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

N

K

R

V

V

E

D

V

I

A

A

K

K

N

N

I

I

T

T

P

P

S

S

S

A

R
|
R

G

G

E

E

L

L

E

E

I

I

T

T

A

D

V

V

N

N

Q

K

Q

A

F

Y

L

L

N

E

D

L

Q

G

E

E

L

L

K

H

V

V

Q

E

L

L

G
|
G

R
|
R

G
|
G

F

F

A

A

W
|
W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

S

S

L

L

S

L

E

Q

A

A

S

S

T

Q

F

F

I

I

E

E

V

T

I

I

E

E

K

K

R

R

Q

Q

G

S

L

L

K

K

V

V

A

A

C

C

L

L

E

E

G

E

I
|
I

A

A

L

Y

R

R

H

M

G

G

W

Y

I

I

T

S

T

R

D

E

K

Q

M

L

R

I

E

K

L

L

A

A

Q

E

P

P

M

L

L

M

K

K

N

N

Q

E

Y

Y

G

G

Q

Q

Y

Y

L

L

L

M

K

N

V

L

binding galactose-uridine-5'-diphosphate: L6 (= L6), G8 (= G8), G9 (= G9), Q80 (= Q80), P83 (= P83), G85 (= G85), L86 (= L86), L106 (= L106), D108 (= D108), G144 (= G147), E159 (= E162), K160 (= K163), V170 (= V173), R192 (= R195), W221 (= W224)
binding uridine 5'-triphosphate: L43 (= L43), G113 (= G113), H114 (≠ Q114), G115 (= G115), T117 (= T117), G216 (= G219), R217 (= R220), G218 (= G221), I254 (= I257)

4ho6A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with udp-glucose and utp
68% identity, 98% coverage: 1:288/295 of query aligns to 1:285/288 of 4ho6A

query
sites
4ho6A

M

M

K

K

G

G

I

I

V

I

L
|
L

A

S

G
|
G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

I

L

T

T

K

K

G

V

V

V

S

S

K

K

Q

Q

L

L

P

P

I

V

F

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

I

L

S

S

V

V

L

L

M

M

L
|
L

A

A

G

G

I

I

R

K

E

D

I

I

L

L

I

I

S

S

T

T

P

P

Y

E

D

D

L

T

P

P

G

R

F

F

K

E

R

Q

L

L

G

G

D

G

G

G

S

S

D

E

Y

L

G

G

V

I

H

S

F

L

E

S

Y

Y

A

A

E

V

Q
|
Q

P

S

S

S

P
|
P

D

D

G
|
G

L

L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

N

D

D

D

S

N

V

V

C

A

L

L

V

V

L

L

G

G

D
|
D

N

N

I

I

F

F

Y
|
Y

G
|
G

Q
x
H

G
|
G

F

F

T
|
T

H

E

M

L

L

L

K

Q

E

R

A

A

V

-

H

-

T

-

A

A

E

N

E

R

E

K

N

S

K

G

A

A

T

T

V

I

F

F

G

G

Y

Y

W

N

V

V

A

K

D

D

P

P

E

Q

R

R

Y

F

G
|
G

V

V

A

V

E

E

F

F

D

D

K

E

I

K

G

G

N

K

V

V

L

I

S

S

I

I

E

E

E
|
E

K
|
K

P

P

I

E

Q

E

P

P

K

K

S

S

N

S

Y

Y

A

A

V
|
V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

N

K

R

V

V

E

D

V

I

A

A

K

K

N

N

I

I

T

T

P

P

S

S

S

A

R
|
R

G

G

E

E

L

L

E

E

I

I

T

T

A

D

V

V

N

N

Q

K

Q

A

F

Y

L

L

N

E

D

L

Q

G

E

E

L

L

K

H

V

V

Q

E

L

L

G

G

R

R

G

G

F

F

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

S

S

L

L

S

L

E

Q

A

A

S

S

T

Q

F

F

I

I

E

E

V

T

I

I

E

E

K

K

R

R

Q

Q

G

S

L

L

K

K

V

V

A

A

C

C

L

L

E

E

G
x
E

I
|
I

A

A

L

Y

R

R

H

M

G

G

W

Y

I

I

T

S

T

R

D

E

K

Q

M

L

R

I

E

K

L

L

A

A

Q

E

P

P

M

L

L

M

K

K

N

N

Q

E

Y

Y

G

G

Q

Q

Y

Y

L

L

L

M

K

N

V

L

binding uridine-5'-diphosphate-glucose: L6 (= L6), G8 (= G8), G9 (= G9), Q80 (= Q80), P83 (= P83), G85 (= G85), D108 (= D108), G144 (= G147), E159 (= E162), K160 (= K163), V170 (= V173), R192 (= R195)
binding uridine 5'-triphosphate: L43 (= L43), Y112 (= Y112), G113 (= G113), H114 (≠ Q114), G115 (= G115), T117 (= T117), E253 (≠ G256), I254 (= I257)

4ho5A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with tdp-glucose
68% identity, 98% coverage: 1:288/295 of query aligns to 1:285/288 of 4ho5A

query
sites
4ho5A

M

M

K

K

G

G

I

I

V

I

L
|
L

A

S

G
|
G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

I

L

T

T

K

K

G

V

V

V

S

S

K

K

Q
|
Q

L

L

P

P

I

V

F

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

I

L

S

S

V

V

L

L

M

M

L

L

A

A

G

G

I

I

R

K

E

D

I

I

L

L

I

I

S

S

T

T

P

P

Y

E

D

D

L

T

P

P

G

R

F

F

K

E

R

Q

L

L

G

G

D

G

G

G

S

S

D

E

Y

L

G

G

V

I

H

S

F

L

E

S

Y

Y

A

A

E

V

Q
|
Q

P

S

S

S

P
|
P

D

D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

N

D

D

D

S

N

V

V

C

A

L

L

V

V

L
|
L

G

G

D
|
D

N

N

I

I

F

F

Y
|
Y

G
|
G

Q
x
H

G
|
G

F
|
F

T
|
T

H

E

M

L

L

L

K

Q

E

R

A

A

V

-

H

-

T

-

A

A

E

N

E

R

E

K

N

S

K

G

A

A

T

T

V

I

F

F

G

G

Y

Y

W

N

V

V

A

K

D

D

P

P

E

Q

R

R

Y
x
F

G
|
G

V

V

A

V

E

E

F

F

D

D

K

E

I

K

G

G

N

K

V

V

L

I

S

S

I

I

E

E

E
|
E

K
|
K

P

P

I

E

Q

E

P

P

K

K

S

S

N

S

Y

Y

A

A

V
|
V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

N

K

R

V

V

E

D

V

I

A

A

K

K

N

N

I

I

T

T

P

P

S

S

S

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

A

D

V

V

N

N

Q

K

Q

A

F

Y

L

L

N

E

D

L

Q

G

E

E

L

L

K

H

V

V

Q

E

L

L

G
|
G

R
|
R

G
|
G

F

F

A

A

W
|
W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

S

S

L

L

S

L

E

Q

A

A

S

S

T

Q

F

F

I

I

E

E

V

T

I

I

E

E

K

K

R

R

Q

Q

G

S

L

L

K

K

V

V

A

A

C

C

L

L

E

E

G
x
E

I
|
I

A

A

L

Y

R

R

H

M

G

G

W

Y

I

I

T

S

T

R

D

E

K

Q

M

L

R

I

E

K

L

L

A

A

Q

E

P

P

M

L

L

M

K

K

N

N

Q

E

Y

Y

G

G

Q

Q

Y

Y

L

L

L

M

K

N

V

L

binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: L6 (= L6), G8 (= G8), G9 (= G9), Q24 (= Q24), Q80 (= Q80), P83 (= P83), G85 (= G85), L86 (= L86), L106 (= L106), D108 (= D108), Y112 (= Y112), G113 (= G113), H114 (≠ Q114), G115 (= G115), F116 (= F116), T117 (= T117), F143 (≠ Y146), G144 (= G147), E159 (= E162), K160 (= K163), V170 (= V173), G216 (= G219), R217 (= R220), G218 (= G221), W221 (= W224), E253 (≠ G256), I254 (= I257)

4ho3A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with thymidine triphosphate
68% identity, 98% coverage: 1:288/295 of query aligns to 1:285/288 of 4ho3A

query
sites
4ho3A

M

M

K

K

G

G

I

I

V

I

L
|
L

A

S

G
|
G

S

S

G
|
G

T
|
T

R
|
R

L

L

Y

Y

P

P

I

L

T

T

K

K

G

V

V

V

S

S

K

K

Q
|
Q

L

L

P

P

I

V

F

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

I

L

S

S

V

V

L

L

M

M

L
|
L

A

A

G

G

I

I

R

K

E

D

I

I

L

L

I

I

S

S

T

T

P

P

Y

E

D

D

L

T

P

P

G

R

F

F

K

E

R

Q

L

L

G

G

D

G

G

G

S

S

D

E

Y

L

G

G

V

I

H

S

F

L

E

S

Y

Y

A

A

E

V

Q
|
Q

P

S

S

S

P
|
P

D

D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

N

D

D

D

S

N

V

V

C

A

L

L

V

V

L

L

G

G

D
|
D

N

N

I

I

F

F

Y
|
Y

G
|
G

Q
x
H

G
|
G

F
|
F

T
|
T

H

E

M

L

L

L

K

Q

E

R

A

A

V

-

H

-

T

-

A

A

E

N

E

R

E

K

N

S

K

G

A

A

T

T

V

I

F

F

G

G

Y

Y

W

N

V

V

A

K

D

D

P

P

E

Q

R

R

Y

F

G

G

V

V

A

V

E

E

F

F

D

D

K

E

I

K

G

G

N

K

V

V

L

I

S

S

I

I

E

E

K

K

P

P

I

E

Q

E

P

P

K

K

S

S

N

S

Y

Y

A

A

V

V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

N

K

R

V

V

E

D

V

I

A

A

K

K

N

N

I

I

T

T

P

P

S

S

S

A

R

R

G

G

E
|
E

L

L

E

E

I

I

T

T

A

D

V

V

N

N

Q

K

Q

A

F

Y

L

L

N

E

D

L

Q

G

E

E

L

L

K

H

V

V

Q

E

L

L

G

G

R

R

G

G

F

F

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

S

S

L

L

S

L

E

Q

A

A

S

S

T

Q

F

F

I

I

E

E

V

T

I

I

E

E

K

K

R

R

Q

Q

G

S

L

L

K

K

V

V

A

A

C

C

L

L

E

E

G
x
E

I
|
I

A

A

L

Y

R
|
R

H

M

G

G

W

Y

I

I

T

S

T

R

D

E

K

Q

M

L

R

I

E

K

L

L

A

A

Q

E

P

P

M

L

L

M

K

K

N

N

Q

E

Y

Y

G

G

Q

Q

Y

Y

L

L

L

M

K

N

V

L

binding thymidine-5'-triphosphate: L6 (= L6), G8 (= G8), G9 (= G9), G11 (= G11), T12 (= T12), R13 (= R13), Q24 (= Q24), L43 (= L43), Q80 (= Q80), P83 (= P83), G85 (= G85), L86 (= L86), D108 (= D108), Y112 (= Y112), G113 (= G113), H114 (≠ Q114), G115 (= G115), F116 (= F116), T117 (= T117), E194 (= E197), E253 (≠ G256), I254 (= I257), R257 (= R260)

4ho4A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with thymidine and glucose-1-phosphate
68% identity, 98% coverage: 1:288/295 of query aligns to 1:285/289 of 4ho4A

query
sites
4ho4A

M

M

K

K

G

G

I

I

V

I

L

L

A

S

G

G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

I

L

T

T

K

K

G

V

V

V

S

S

K

K

Q

Q

L

L

P

P

I

V

F

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

I

L

S

S

V

V

L

L

M

M

L

L

A

A

G

G

I

I

R

K

E

D

I

I

L

L

I

I

S

S

T

T

P

P

Y

E

D

D

L

T

P

P

G

R

F

F

K

E

R

Q

L

L

G

G

D

G

G

G

S

S

D

E

Y

L

G

G

V

I

H

S

F

L

E

S

Y

Y

A

A

E

V

Q

Q

P

S

S

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

N

D

D

D

S

N

V

V

C

A

L

L

V

V

L
|
L

G

G

D

D

N

N

I

I

F

F

Y

Y

G

G

Q

H

G

G

F

F

T

T

H

E

M

L

L

L

K

Q

E

R

A

A

V

-

H

-

T

-

A

A

E

N

E

R

E

K

N

S

K

G

A

A

T

T

V

I

F

F

G

G

Y

Y

W

N

V

V

A

K

D

D

P

P

E

Q

R

R

Y
x
F

G
|
G

V

V

A

V

E

E

F

F

D

D

K

E

I

K

G

G

N

K

V

V

L

I

S

S

I

I

E

E

E
|
E

K
|
K

P

P

I

E

Q

E

P

P

K

K

S

S

N

S

Y

Y

A

A

V
|
V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

N

K

R

V

V

E

D

V

I

A

A

K

K

N

N

I

I

T

T

P

P

S

S

S

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

A

D

V

V

N

N

Q

K

Q

A

F

Y

L

L

N

E

D

L

Q

G

E

E

L

L

K

H

V

V

Q

E

L

L

G

G

R

R

G

G

F

F

A

A

W
|
W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

S

S

L

L

S

L

E

Q

A

A

S

S

T

Q

F

F

I

I

E

E

V

T

I

I

E

E

K

K

R

R

Q

Q

G

S

L

L

K

K

V

V

A

A

C

C

L

L

E

E

G

E

I

I

A

A

L

Y

R

R

H

M

G

G

W

Y

I

I

T

S

T

R

D

E

K

Q

M

L

R

I

E

K

L

L

A

A

Q

E

P

P

M

L

L

M

K

K

N

N

Q

E

Y

Y

G

G

Q

Q

Y

Y

L

L

L

M

K

N

V

L

binding 1-O-phosphono-alpha-D-glucopyranose: L106 (= L106), F143 (≠ Y146), G144 (= G147), E159 (= E162), K160 (= K163), V170 (= V173), W221 (= W224)

4hocA Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with udp-n- acetylglucosamine
68% identity, 98% coverage: 1:288/295 of query aligns to 1:283/286 of 4hocA

query
sites
4hocA

M

M

K

K

G

G

I

I

V

I

L
|
L

A

S

G
|
G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

I

L

T

T

K

K

G

V

V

V

S

S

K

K

Q

Q

L

L

P

P

I

V

F

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

I

L

S

S

V

V

L

L

M

M

L

L

A

A

G

G

I

I

R

K

E

D

I

I

L

L

I

I

S

S

T

T

P

P

Y

E

D

D

L

T

P

P

G

R

F

F

K

E

R

Q

L

L

G

G

D

G

G

G

S

S

D

E

Y

L

G

G

V

I

H

S

F

L

E

S

Y

Y

A

A

E

V

Q
|
Q

P

S

S

S

P
|
P

D

D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

N

D

D

D

S

N

V

V

C

A

L

L

V

V

L

L

G

G

D
|
D

N

N

I

I

F

F

Y

Y

G

G

Q

H

G

G

F

F

T

T

H

E

M

L

L

L

K

Q

E

R

A

A

V

-

H

-

T

-

A

A

E

N

E

R

E

K

N

S

K

G

A

A

T

T

V

I

F

F

G

G

Y

Y

W

N

V

V

A

K

D

D

P

P

E

Q

R

R

Y

F

G
|
G

V

V

A

V

E

E

F

F

D

D

K

E

I

K

G

G

N

K

V

V

L

I

S

S

I

I

E

E

E
|
E

K
|
K

P

P

I

E

Q

E

P

P

K

K

S

S

N

S

Y

Y

A

A

V
|
V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

N

K

R

V

V

E

D

V

I

A

A

K

K

N

N

I

I

T

T

P

P

S

S

S

-

R

-

G

G

E

E

L

L

E

E

I

I

T

T

A

D

V

V

N

N

Q

K

Q

A

F

Y

L

L

N

E

D

L

Q

G

E

E

L

L

K

H

V

V

Q

E

L

L

G

G

R

R

G

G

F

F

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

E

S

S

L

L

S

L

E

Q

A

A

S

S

T

Q

F

F

I

I

E

E

V

T

I

I

E

E

K

K

R

R

Q

Q

G

S

L

L

K

K

V

V

A

A

C

C

L

L

E

E

G

E

I

I

A

A

L

Y

R

R

H

M

G

G

W

Y

I

I

T

S

T

R

D

E

K

Q

M

L

R

I

E

K

L

L

A

A

Q

E

P

P

M

L

L

M

K

K

N

N

Q

E

Y

Y

G

G

Q

Q

Y

Y

L

L

L

M

K

N

V

L

binding uridine-diphosphate-n-acetylglucosamine: L6 (= L6), G8 (= G8), G9 (= G9), Q80 (= Q80), P83 (= P83), G85 (= G85), L86 (= L86), D108 (= D108), G144 (= G147), E159 (= E162), K160 (= K163), V170 (= V173)

1mc3A Crystal structure of rffh (see paper)
67% identity, 98% coverage: 1:289/295 of query aligns to 2:287/291 of 1mc3A

query
sites
1mc3A

M

M

K

K

G

G

I

I

V

I

L
|
L

A

A

G
|
G

S

S

G
|
G

T
|
T

R
|
R

L

L

Y

H

P

P

I

I

T

T

K

R

G

G

V

V

S

S

K
|
K

Q
|
Q

L

L

P

P

I

I

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

V

L

L

M

M

L

L

A

A

G

G

I

I

R

R

E

E

I

I

L

L

I

I

S

T

T

T

P

P

Y

E

D

D

L

K

P

G

G

Y

F

F

K

Q

R

R

L

L

G

G

D

D

G

G

S

S

D

E

Y

F

G

G

V

I

H

Q

F

L

E

E

Y

Y

A

A

E

E

Q
|
Q

P

P

S

S

P
|
P

D

D

G

G

L
|
L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

E

T

F

F

I

L

G

N

N

G

D

E

S

P

V

S

C

C

L

L

V

V

L

L

G

G

D
|
D

N

N

I

I

F

F

Y

F

G

G

Q

Q

G

G

F

F

T

S

H

P

M

K

L

L

K

R

E

-

A

-

V

-

H

H

T

V

A

A

E

A

E

R

E

T

N

E

K

G

A

A

T

T

V

V

F

F

G

G

Y

Y

W

Q

V

V

A

M

D

D

P

P

E

E

R

R

Y

F

G

G

V

V

A

V

E

E

F

F

D

D

K

D

I

N

G

F

N

R

V

A

L

I

S

S

I

L

E

E

K

K

P

P

I

K

Q

Q

P

P

K

K

S

S

N

N

Y

W

A

A

V

V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

S

K

K

V

V

E

E

V

Y

A

A

K

K

N

Q

I

V

T

K

P

P

S

S

S

E

R

R

G

G

E

E

L

L

E

E

I

I

T

T

A

S

V

I

N

N

Q

Q

Q

M

F

Y

L

L

N

E

D

A

Q

G

E

N

L

L

K

T

V

V

Q

E

L

L

G

G

R

R

G

G

F

F

A

A

W

W

L

L

D
|
D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

S

I

E

E

A

A

S

S

T

T

F

F

I

V

E

Q

V

T

I

V

E

E

K

K

R

R

Q

Q

G

G

L

F

K

K

V

I

A

A

C

C

L

L

E

E

G

E

I

I

A

A

L

W

R

R

H

N

G

G

W

W

I

L

T

D

D

E

K

G

M

V

R

K

E

R

L

A

A

A

Q

S

P

S

M

L

L

A

K

K

N

T

Q

G

Y

Y

G

G

Q

Q

Y

Y

L

L

K

E

V

L

I

L

binding magnesium ion: D109 (= D108), D224 (= D226)
binding thymidine-5'-triphosphate: L7 (= L6), G9 (= G8), G10 (= G9), G12 (= G11), T13 (= T12), R14 (= R13), K24 (= K23), Q25 (= Q24), Q81 (= Q80), P84 (= P83), L87 (= L86), D109 (= D108)

P61887 Glucose-1-phosphate thymidylyltransferase 2; G1P-TT 2; dTDP-glucose pyrophosphorylase 2; dTDP-glucose synthase 2; EC 2.7.7.24 from Escherichia coli (strain K12) (see paper)
67% identity, 98% coverage: 1:289/295 of query aligns to 1:286/293 of P61887

query
sites
P61887

M

M

K

K

G

G

I

I

V

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

Y

H

P

P

I

I

T

T

K

R

G

G

V

V

S

S

K

K

Q

Q

L

L

P

P

I

I

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

V

L

L

M

M

L

L

A

A

G

G

I

I

R

R

E

E

I

I

L

L

I

I

S

T

T

T

P

P

Y

E

D

D

L

K

P

G

G

Y

F

F

K

Q

R

R

L

L

G

G

D

D

G

G

S

S

D

E

Y

F

G

G

V

I

H

Q

F

L

E

E

Y

Y

A

A

E

E

Q

Q

P

P

S

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

E

T

F

F

I

L

G

N

N

G

D

E

S

P

V

S

C

C

L

L

V

V

L

L

G

G

D
|
D

N

N

I

I

F

F

Y

F

G

G

Q

Q

G

G

F

F

T

S

H

P

M

K

L

L

K

R

E

-

A

-

V

-

H

H

T

V

A

A

E

A

E

R

E

T

N

E

K

G

A

A

T

T

V

V

F

F

G

G

Y

Y

W

Q

V

V

A

M

D

D

P

P

E

E

R

R

Y

F

G

G

V

V

A

V

E

E

F

F

D

D

K

D

I

N

G

F

N

R

V

A

L

I

S

S

I

L

E

E

K

K

P

P

I

K

Q

Q

P

P

K

K

S

S

N

N

Y

W

A

A

V

V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

S

K

K

V

V

E

E

V

Y

A

A

K

K

N

Q

I

V

T

K

P

P

S

S

S

E

R

R

G

G

E

E

L

L

E

E

I

I

T

T

A

S

V

I

N

N

Q

Q

Q

M

F

Y

L

L

N

E

D

A

Q

G

E

N

L

L

K

T

V

V

Q

E

L

L

G

G

R

R

G

G

F

F

A

A

W

W

L

L

D
|
D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

S

I

E

E

A

A

S

S

T

T

F

F

I

V

E

Q

V

T

I

V

E

E

K

K

R

R

Q

Q

G

G

L

F

K

K

V

I

A

A

C

C

L

L

E

E

G

E

I

I

A

A

L

W

R

R

H

N

G

G

W

W

I

L

T

D

D

E

K

G

M

V

R

K

E

R

L

A

A

A

Q

S

P

S

M

L

L

A

K

K

N

T

Q

G

Y

Y

G

G

Q

Q

Y

Y

L

L

K

E

V

L

I

L

D108 (= D108) binding
D223 (= D226) binding

1h5sB Thymidylyltransferase complexed with tmp (see paper)
66% identity, 97% coverage: 2:287/295 of query aligns to 5:287/291 of 1h5sB

query
sites
1h5sB

K

K

G

G

I

I

V

I

L
|
L

A

A

G
|
G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

I

V

T

T

K

M

G

A

V

V

S

S

K

K

Q

Q

L

L

P

P

I

I

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

T

L

L

M

M

L

L

A

A

G

G

I

I

R

R

E

D

I

I

L

L

I

I

S

S

T

T

P

P

Y

Q

D

D

L

T

P

P

G

R

F

F

K

Q

R

Q

L

L

G

G

D

D

G

G

S

S

D

Q

Y

W

G

G

V

L

H

N

F

L

E

Q

Y

Y

A

K

E

V

Q
|
Q

P

P

S

S

P
|
P

D
|
D

G
|
G

L

L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

N

G

D

D

S

D

V

C

C

A

L

L

V

V

L

L

G

G

D
|
D

N

N

I

I

F

F

Y
|
Y

G
|
G

Q
x
H

G
x
D

F

L

T

P

H

K

M

L

L

M

K

E

E

A

A

A

V

V

H

N

T

-

A

-

E

-

E

K

E

E

N

S

K

G

A

A

T

T

V

V

F

F

G

A

Y

Y

W

H

V

V

A

N

D

D

P

P

E

E

R

R

Y

Y

G

G

V

V

A

V

E

E

F

F

D

D

K

K

I

N

G

G

N

T

V

A

L

I

S

S

I

L

E

E

K

K

P

P

I

L

Q

E

P

P

K

K

S

S

N

N

Y

Y

A

A

V

V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

N

K

D

V

V

E

Q

V

M

A

A

K

K

N

N

I

L

T

K

P

P

S

S

S

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

A

D

V

I

N

N

Q

R

Q

I

F

Y

L

L

N

E

D

Q

Q

G

E

R

L

L

K

S

V

V

Q

A

L

L

L

M

G
|
G

R
|
R

G
|
G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

Q

S

S

L

L

S

I

E

E

A

A

S

S

T

N

F

F

I

I

E

A

V

T

I

I

E

E

K

E

R

R

Q

Q

G

G

L

L

K

K

V

V

A
x
S

C

C

L

P

E

E

G
x
E

I
|
I

A

A

L

F

R
|
R

H

K

G

G

W

F

I

I

T

D

T

V

D

E

K

Q

M

V

R

R

E

K

L

L

A

A

Q

V

P

P

M

L

L

I

K

K

N

N

Q

N

Y

Y

G

G

Q

Q

Y

Y

L

L

L

Y

K

K

binding thymidine-5'-phosphate: L9 (= L6), G11 (= G8), G12 (= G9), Q83 (= Q80), P86 (= P83), D87 (= D84), G88 (= G85), D111 (= D108), Y115 (= Y112), G116 (= G113), H117 (≠ Q114), D118 (≠ G115), G219 (= G219), R220 (= R220), G221 (= G221), S252 (≠ A252), E256 (≠ G256), I257 (= I257), R260 (= R260)

1h5tA Thymidylyltransferase complexed with thymidylyldiphosphate-glucose (see paper)
65% identity, 98% coverage: 2:291/295 of query aligns to 4:290/290 of 1h5tA

query
sites
1h5tA

K

K

G

G

I

I

V

I

L
|
L

A

A

G
|
G

G

G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

I

V

T

T

K

M

G

A

V

V

S

S

K

K

Q

Q

L

L

P

P

I

I

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

T

L

L

M

M

L

L

A

A

G

G

I

I

R

R

E

D

I

I

L

L

I

I

S

S

T

T

P

P

Y

Q

D

D

L

T

P

P

G

R

F

F

K

Q

R

Q

L

L

G

G

D

D

G

G

S

S

D

Q

Y

W

G

G

V

L

H

N

F

L

E

Q

Y

Y

A

K

E

V

Q
|
Q

P

P

S

S

P
|
P

D

D

G
|
G

L

L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

N

G

D

D

S

D

V

C

C

A

L

L

V

V

L
|
L

G

G

D
|
D

N

N

I

I

F

F

Y
|
Y

G
|
G

Q
x
H

G
x
D

F

L

T

P

H

K

M

L

L

M

K

E

E

A

A

A

V

V

H

N

T

-

A

-

E

-

E

K

E

E

N

S

K

G

A

A

T

T

V

V

F

F

G

A

Y

Y

W

H

V

V

A

N

D

D

P

P

E

E

R

R

Y
|
Y

G
|
G

V

V

A

V

E

E

F

F

D

D

K

K

I

N

G

G

N

T

V

A

L

I

S

S

I

L

E

E

E
|
E

K
|
K

P

P

I

L

Q

E

P

P

K

K

S

S

N

N

Y

Y

A

A

V
|
V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

N

K

D

V

V

E

Q

V

M

A

A

K

K

N

N

I

L

T

K

P

P

S

S

S

A

R
|
R

G

G

E

E

L

L

E

E

I

I

T

T

A

D

V

I

N

N

Q

R

Q

I

F

Y

L

L

N

E

D

Q

Q

G

E

R

L

L

K

S

V

V

Q

A

L

M

G

G

R
|
R

G
|
G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

Q

S

S

L

L

S

I

E

E

A

A

S

S

T

N

F

F

I

I

E

A

V

T

I

I

E

E

K

E

R

R

Q

Q

G

G

L

L

K

K

V
|
V

A
x
S

C

C

L

P

E

E

G

E

I

I

A

A

L

F

R

R

H

K

G

G

W

F

I

I

T

D

T

V

D

E

K

Q

M

V

R

R

E

K

L

L

A

A

Q

V

P

P

M

L

L

I

K

K

N

N

Q

N

Y

Y

G

G

Q

Q

Y

Y

L

L

L

Y

K

K

V

M

I

T

D

K

E

D

binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: L8 (= L6), G10 (= G8), Q82 (= Q80), P85 (= P83), G87 (= G85), L108 (= L106), D110 (= D108), Y145 (= Y146), G146 (= G147), E161 (= E162), K162 (= K163), V172 (= V173), R194 (= R195)
binding thymidine-5'-diphosphate: Y114 (= Y112), G115 (= G113), H116 (≠ Q114), D117 (≠ G115), R219 (= R220), G220 (= G221), V250 (= V251), S251 (≠ A252)

1h5rA Thymidylyltransferase complexed with thimidine and glucose-1-phospate (see paper)
65% identity, 98% coverage: 2:291/295 of query aligns to 4:290/290 of 1h5rA

query
sites
1h5rA

K

K

G

G

I

I

V

I

L
|
L

A

A

G
|
G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

I

V

T

T

K

M

G

A

V

V

S

S

K

K

Q

Q

L

L

P

P

I

I

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

T

L

L

M

M

L

L

A

A

G

G

I

I

R

R

E

D

I

I

L

L

I

I

S

S

T

T

P

P

Y

Q

D

D

L

T

P

P

G

R

F

F

K

Q

R

Q

L

L

G

G

D

D

G

G

S

S

D

Q

Y

W

G

G

V

L

H

N

F

L

E

Q

Y

Y

A

K

E

V

Q
|
Q

P

P

S

S

P
|
P

D

D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

N

G

D

D

S

D

V

C

C

A

L

L

V

V

L

L

G

G

D
|
D

N

N

I

I

F

F

Y
|
Y

G
|
G

Q

H

G

D

F

L

T

P

H

K

M

L

L

M

K

E

E

A

A

A

V

V

H

N

T

-

A

-

E

-

E

K

E

E

N

S

K

G

A

A

T

T

V

V

F

F

G

A

Y

Y

W

H

V

V

A

N

D

D

P

P

E

E

R

R

Y
|
Y

G
|
G

V

V

A

V

E

E

F

F

D

D

K

K

I

N

G

G

N

T

V

A

L

I

S

S

I

L

E

E

E
|
E

K
|
K

P

P

I

L

Q

E

P

P

K

K

S

S

N

N

Y

Y

A

A

V
|
V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

N

K

D

V

V

E

Q

V

M

A

A

K

K

N

N

I

L

T

K

P

P

S

S

S

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

A

D

V

I

N

N

Q

R

Q

I

F

Y

L

L

N

E

D

Q

Q

G

E

R

L

L

K

S

V

V

Q

A

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

Q

S

S

L

L

S

I

E

E

A

A

S

S

T

N

F

F

I

I

E

A

V

T

I

I

E

E

K

E

R

R

Q

Q

G

G

L

L

K

K

V
|
V

A
x
S

C

C

L

P

E

E

G
x
E

I
|
I

A

A

L

F

R

R

H

K

G

G

W

F

I

I

T

D

T

V

D

E

K

Q

M

V

R

R

E

K

L

L

A

A

Q

V

P

P

M

L

L

I

K

K

N

N

Q

N

Y

Y

G

G

Q

Q

Y

Y

L

L

L

Y

K

K

V

M

I

T

D

K

E

D

binding 1-O-phosphono-alpha-D-glucopyranose: L88 (= L86), Y145 (= Y146), G146 (= G147), E161 (= E162), K162 (= K163), V172 (= V173)
binding thymidine: L8 (= L6), G10 (= G8), G11 (= G9), Q82 (= Q80), P85 (= P83), G87 (= G85), L88 (= L86), D110 (= D108), Y114 (= Y112), G115 (= G113), V250 (= V251), S251 (≠ A252), E255 (≠ G256), I256 (= I257)

P26393 Glucose-1-phosphate thymidylyltransferase; dTDP-glucose pyrophosphorylase; Ep; dTDP-glucose synthase; EC 2.7.7.24 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
65% identity, 99% coverage: 2:292/295 of query aligns to 5:292/292 of P26393

query
sites
P26393

K

K

G

G

I

I

V

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

I

V

T

T

K

M

G

A

V

V

S

S

K

K

Q

Q

L

L

P

P

I

I

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

T

L

L

M

M

L

L

A

A

G

G

I

I

R

R

E

D

I

I

L

L

I

I

S

S

T

T

P

P

Y

Q

D

D

L

T

P

P

G

R

F

F

K

Q

R

Q

L

L

G

G

D

D

G

G

S

S

D

Q

Y

W

G

G

V

L

H

N

F

L

E

Q

Y

Y

A

K

E

V

Q

Q

P

P

S

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

N

H

D

D

S

D

V

C

C

A

L

L

V

V

L

L

G

G

D

D

N

N

I

I

F

F

Y

Y

G

G

Q

H

G

D

F

L

T

P

H

K

M

L

L

M

K

E

E

A

A

A

V

V

H

N

T

-

A

-

E

-

E

K

E

E

N

S

K

G

A

A

T

T

V

V

F

F

G

A

Y

Y

W

H

V

V

A

N

D

D

P

P

E

E

R

R

Y

Y

G

G

V

V

A

V

E

E

F

F

D

D

K

Q

I

K

G

G

N

T

V

A

L

V

S

S

I

L

E

E

K

K

P

P

I

L

Q

Q

P

P

K

K

S

S

N

N

Y

Y

A

A

V

V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

N

K

S

V

V

E

E

V

M

A

A

K

K

N

N

I

L

T

K

P

P

S

S

S

A

R

R

G

G

E

E

L

L

E

E

I

I

T
|
T

A

D

V

I

N

N

Q

R

Q

I

F

Y

L

M

N

E

D

Q

Q

G

E

R

L

L

K

S

V

V

Q

A

L

M

G

G

R

R

G

G

F

Y

A

A

W
|
W

L

L

D

D

T

T

G

G

T

T

H

H

D

Q

S

S

L

L

S

I

E

E

A

A

S

S

T

N

F

F

I

I

E

A

V

T

I

I

E

E

K

E

R

R

Q

Q

G

G

L

L

K

K

V

V

A

S

C

C

L

P

E

E

G

E

I

I

A

A

L

F

R

R

H

K

G

N

W

F

I

I

T

N

T

A

D

Q

K

Q

M

V

R

I

E

E

L

L

A

A

Q

G

P

P

M

L

L

S

K

K

N

N

Q

D

Y

Y

G

G

Q

K

Y

Y

L

L

K

K

V

M

I

V

D

K

E

G

L

L

T201 (= T201) mutation to A: Two-fold increase in the conversion of 2-acetamido-2-deoxy-alpha-D-glucopyranosyl phosphate.
W224 (= W224) mutation to H: Is able to convert both 6-acetamido-6-deoxy-alpha-D-glucopyranosyl phosphate and alpha-D-glucopyranuronic acid 1-(dihydrogen phosphate), which are not accepted by the wild-type.

1iinA Thymidylyltransferase complexed with udp-glucose (see paper)
65% identity, 98% coverage: 2:289/295 of query aligns to 5:289/289 of 1iinA

query
sites
1iinA

K

K

G

G

I

I

V

I

L
|
L

A

A

G
|
G

S

S

G

G

T

T

R
|
R

L

L

Y

Y

P

P

I

V

T

T

K

M

G

A

V

V

S

S

K

Q

Q

Q

L

L

P

P

I

I

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

T

L

L

M

M

L

L

A

A

G

G

I

I

R

R

E

D

I

I

L

L

I

I

S

S

T

T

P

P

Y

Q

D

D

L

T

P

P

G

R

F

F

K

Q

R

Q

L

L

G

G

D

D

G

G

S

S

D

Q

Y

W

G

G

V

L

H

N

F

L

E

Q

Y

Y

A

K

E

V

Q
|
Q

P

P

S

S

P
|
P

D
|
D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

N

H

D

D

S

D

V

C

C

A

L

L

V

V

L
|
L

G

G

D
|
D

N

N

I

I

F

F

Y

Y

G

G

Q

H

G

D

F

L

T

P

H

K

M

L

L

M

K

E

E

A

A

A

V

V

H

N

T

-

A

-

E

-

E

K

E

E

N

S

K

G

A

A

T

T

V

V

F

F

G

A

Y

Y

W

H

V

V

A

N

D

D

P

P

E

E

R

R

Y
|
Y

G
|
G

V

V

A

V

E

E

F

F

D

D

K

Q

I

K

G

G

N

T

V

A

L

V

S

S

I

L

E

E

E
|
E

K
|
K

P

P

I

L

Q

Q

P

P

K

K

S

S

N

N

Y

Y

A

A

V
|
V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

N

K

S

V

V

E

E

V

M

A

A

K

K

N

N

I

L

T

K

P

P

S

S

S

A

R
|
R

G

G

E

E

L

L

E

E

I

I

T

T

A

D

V

I

N

N

Q

R

Q

I

F

Y

L

M

N

E

D

Q

Q

G

E

R

L

L

K

S

V

V

Q

A

L

M

G

G

R

R

G

G

F

Y

A

A

W
|
W

L

L

D

D

T

T

G

G

T

T

H

H

D

Q

S

S

L

L

S

I

E

E

A

A

S

S

T

N

F

F

I

I

E

A

V

T

I

I

E

E

K

E

R

R

Q

Q

G

G

L

L

K

K

V

V

A

S

C

C

L

P

E

E

G

E

I

I

A

A

L

F

R

R

H

K

G

N

W

F

I

I

T

N

T

A

D

Q

K

Q

M

V

R

I

E

E

L

L

A

A

Q

G

P

P

M

L

L

S

K

K

N

N

Q

D

Y

Y

G

G

Q

K

Y

Y

L

L

K

K

V

M

I

V

binding uridine-5'-diphosphate-glucose: L9 (= L6), G11 (= G8), G12 (= G9), R16 (= R13), Q83 (= Q80), P86 (= P83), D87 (= D84), G88 (= G85), L89 (= L86), L109 (= L106), D111 (= D108), Y146 (= Y146), G147 (= G147), E162 (= E162), K163 (= K163), V173 (= V173), R195 (= R195), W224 (= W224)

1iimA Thymidylyltransferase complexed with ttp (see paper)
65% identity, 98% coverage: 2:289/295 of query aligns to 5:289/289 of 1iimA

query
sites
1iimA

K

K

G

G

I

I

V

I

L
|
L

A

A

G
|
G

S
|
S

G
|
G

T
|
T

R
|
R

L

L

Y

Y

P

P

I

V

T

T

K

M

G

A

V

V

S

S

K

Q

Q
|
Q

L

L

P

P

I

I

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

T

L

L

M

M

L

L

A

A

G

G

I

I

R

R

E

D

I

I

L

L

I

I

S

S

T

T

P

P

Y

Q

D

D

L

T

P

P

G

R

F

F

K

Q

R

Q

L

L

G

G

D

D

G

G

S

S

D

Q

Y

W

G

G

V

L

H

N

F

L

E

Q

Y

Y

A

K

E

V

Q
|
Q

P

P

S

S

P
|
P

D

D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

N

H

D

D

S

D

V

C

C

A

L

L

V

V

L

L

G

G

D
|
D

N

N

I

I

F

F

Y
|
Y

G
|
G

Q
x
H

G

D

F

L

T
x
P

H

K

M

L

L

M

K

E

E

A

A

A

V

V

H

N

T

-

A

-

E

-

E

K

E

E

N

S

K

G

A

A

T

T

V

V

F

F

G

A

Y

Y

W

H

V

V

A

N

D

D

P

P

E

E

R

R

Y

Y

G

G

V

V

A

V

E

E

F

F

D

D

K

Q

I

K

G

G

N

T

V

A

L

V

S

S

I

L

E

E

K

K

P

P

I

L

Q

Q

P

P

K

K

S

S

N

N

Y

Y

A

A

V

V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

N

K

S

V

V

E

E

V

M

A

A

K

K

N

N

I

L

T

K

P

P

S

S

S

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

A

D

V

I

N

N

Q

R

Q

I

F

Y

L

M

N

E

D

Q

Q

G

E

R

L

L

K

S

V

V

Q

A

L

M

G
|
G

R
|
R

G
|
G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

Q

S

S

L

L

S

I

E

E

A

A

S

S

T

N

F

F

I

I

E

A

V

T

I

I

E

E

K

E

R

R

Q

Q

G

G

L

L

K

K

V

V

A
x
S

C

C

L

P

E

E

G
x
E

I
|
I

A

A

L

F

R

R

H

K

G

N

W

F

I

I

T

N

T

A

D

Q

K

Q

M

V

R

I

E

E

L

L

A

A

Q

G

P

P

M

L

L

S

K

K

N

N

Q

D

Y

Y

G

G

Q

K

Y

Y

L

L

K

K

V

M

I

V

binding thymidine-5'-triphosphate: L9 (= L6), G11 (= G8), G12 (= G9), S13 (= S10), G14 (= G11), T15 (= T12), R16 (= R13), Q27 (= Q24), Q83 (= Q80), P86 (= P83), G88 (= G85), L89 (= L86), D111 (= D108), Y115 (= Y112), G116 (= G113), H117 (≠ Q114), P120 (≠ T117), G219 (= G219), R220 (= R220), G221 (= G221), S252 (≠ A252), E256 (≠ G256), I257 (= I257)

3pkpA Q83s variant of s. Enterica rmla with datp (see paper)
65% identity, 98% coverage: 2:289/295 of query aligns to 5:289/290 of 3pkpA

query
sites
3pkpA

K

K

G

G

I

I

V

I

L

L

A

A

G
|
G

S
|
S

G
|
G

T
|
T

R
|
R

L

L

Y

Y

P

P

I

V

T

T

K

M

G

A

V

V

S

S

K
|
K

Q
|
Q

L

L

P

P

I

I

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

T

L

L

M

M

L

L

A

A

G

G

I

I

R

R

E

D

I

I

L

L

I

I

S

S

T

T

P

P

Y

Q

D

D

L

T

P

P

G

R

F

F

K

Q

R

Q

L

L

G

G

D

D

G

G

S

S

D

Q

Y

W

G

G

V

L

H

N

F

L

E

Q

Y

Y

A

K

E

V

Q
x
S

P

P

S

S

P
|
P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

N

H

D

D

S

D

V

C

C

A

L

L

V

V

L

L

G
|
G

D
|
D

N

N

I

I

F

F

Y

Y

G

G

Q

H

G

D

F

L

T

P

H

K

M

L

L

M

K

E

E

A

A

A

V

V

H

N

T

-

A

-

E

-

E

K

E

E

N

S

K

G

A

A

T

T

V

V

F

F

G

A

Y

Y

W

H

V

V

A

N

D

D

P

P

E

E

R

R

Y

Y

G

G

V

V

A

V

E

E

F

F

D

D

K

Q

I

K

G

G

N

T

V

A

L

V

S

S

I

L

E

E

K

K

P

P

I

L

Q

Q

P

P

K

K

S

S

N

N

Y

Y

A

A

V

V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

N

K

S

V

V

E

E

V

M

A

A

K

K

N

N

I

L

T

K

P

P

S

S

S

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

A

D

V

I

N

N

Q

R

Q

I

F

Y

L

M

N

E

D

Q

Q

G

E

R

L

L

K

S

V

V

Q

A

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

Q

S

S

L

L

S

I

E

E

A

A

S

S

T

N

F

F

I

I

E

A

V

T

I

I

E

E

K

E

R

R

Q

Q

G

G

L

L

K

K

V

V

A

S

C

C

L

P

E

E

G

E

I

I

A

A

L

F

R

R

H

K

G

N

W

F

I

I

T

N

T

A

D

Q

K

Q

M

V

R

I

E

E

L

L

A

A

Q

G

P

P

M

L

L

S

K

K

N

N

Q

D

Y

Y

G

G

Q

K

Y

Y

L

L

K

K

V

M

I

V

binding 2'-deoxyadenosine 5'-triphosphate: G11 (= G8), G12 (= G9), S13 (= S10), G14 (= G11), T15 (= T12), R16 (= R13), K26 (= K23), Q27 (= Q24), S83 (≠ Q80), P86 (= P83), G110 (= G107), D111 (= D108)

3pkpB Q83s variant of s. Enterica rmla with datp (see paper)
65% identity, 98% coverage: 2:289/295 of query aligns to 4:288/289 of 3pkpB

query
sites
3pkpB

K

K

G

G

I

I

V

I

L

L

A

A

G
|
G

S
|
S

G
|
G

T
|
T

R
|
R

L

L

Y

Y

P

P

I

V

T

T

K

M

G

A

V

V

S

S

K
|
K

Q
|
Q

L

L

P

P

I

I

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

T

L

L

M

M

L

L

A

A

G

G

I

I

R

R

E

D

I

I

L

L

I

I

S

S

T

T

P

P

Y

Q

D

D

L

T

P

P

G

R

F

F

K

Q

R

Q

L

L

G

G

D

D

G

G

S

S

D

Q

Y

W

G

G

V

L

H

N

F

L

E

Q

Y

Y

A

K

E

V

Q
x
S

P

P

S

S

P
|
P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

N

H

D

D

S

D

V

C

C

A

L

L

V

V

L

L

G
|
G

D
|
D

N

N

I

I

F

F

Y

Y

G

G

Q

H

G

D

F

L

T

P

H

K

M

L

L

M

K

E

E

A

A

A

V

V

H

N

T

-

A

-

E

-

E

K

E

E

N

S

K

G

A

A

T

T

V

V

F

F

G

A

Y

Y

W

H

V

V

A

N

D

D

P

P

E

E

R

R

Y

Y

G

G

V

V

A

V

E

E

F

F

D

D

K

Q

I

K

G

G

N

T

V

A

L

V

S

S

I

L

E

E

K

K

P

P

I

L

Q

Q

P

P

K

K

S

S

N

N

Y

Y

A

A

V

V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

N

K

S

V

V

E

E

V

M

A

A

K

K

N

N

I

L

T

K

P

P

S

S

S

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

A

D

V

I

N

N

Q

R

Q

I

F

Y

L

M

N

E

D

Q

Q

G

E

R

L

L

K

S

V

V

Q

A

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D
|
D

T

T

G

G

T

T

H

H

D

Q

S

S

L

L

S

I

E

E

A

A

S

S

T

N

F

F

I

I

E

A

V

T

I

I

E

E

K

E

R

R

Q

Q

G

G

L

L

K

K

V

V

A

S

C

C

L

P

E

E

G

E

I

I

A

A

L

F

R

R

H

K

G

N

W

F

I

I

T

N

T

A

D

Q

K

Q

M

V

R

I

E

E

L

L

A

A

Q

G

P

P

M

L

L

S

K

K

N

N

Q

D

Y

Y

G

G

Q

K

Y

Y

L

L

K

K

V

M

I

V

binding 2'-deoxyadenosine 5'-triphosphate: G10 (= G8), G11 (= G9), S12 (= S10), G13 (= G11), T14 (= T12), R15 (= R13), K25 (= K23), Q26 (= Q24), S82 (≠ Q80), P85 (= P83), G109 (= G107), D110 (= D108)
binding magnesium ion: D110 (= D108), D225 (= D226)

3pkqA Q83d variant of s. Enterica rmla with dgtp (see paper)
65% identity, 98% coverage: 2:289/295 of query aligns to 3:284/285 of 3pkqA

query
sites
3pkqA

K

K

G

G

I

I

V

I

L
|
L

A

A

G
|
G

S
|
S

G
|
G

T
|
T

R
|
R

L

L

Y

Y

P

P

I

V

T

T

K

M

G

A

V

V

S

S

K

K

Q
|
Q

L

L

P

P

I

I

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V

T

L

L

M

M

L

L

A

A

G

G

I

I

R

R

E

D

I

I

L

L

I

I

S

S

T

T

P

P

Y

Q

D

D

L

T

P

P

G

R

F

F

K

Q

R

Q

L

L

G

G

D

D

G

G

S

S

D

Q

Y

W

G

G

V

L

H

N

F

L

E

Q

Y

Y

A

K

E

V

Q
x
D

P

P

S
|
S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

I

G

G

E

E

F

F

I

I

G

G

N

H

D

D

S

D

V

C

C

A

L

L

V

V

L
|
L

G
|
G

D
|
D

N

N

I

I

F

F

Y

Y

G

G

Q

H

G

D

F

L

T

P

H

K

M

L

L

M

K

E

E

A

A

A

V

V

H

N

T

-

A

-

E

-

E

K

E

E

N

S

K

G

A

A

T

T

V

V

F

F

G

A

Y

Y

W

H

V

V

A

N

D

D

P

P

E

E

R

R

Y

Y

G

G

V

V

A

V

E

E

F

F

D

D

K

-

I

-

G

-

N

T

V

A

L

V

S

S

I

L

E

E

K

K

P

P

I

L

Q

Q

P

P

K

K

S

S

N

N

Y

Y

A

A

V

V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

N

K

S

V

V

E

E

V

M

A

A

K

K

N

N

I

L

T

K

P

P

S

S

S

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

A

D

V

I

N

N

Q

R

Q

I

F

Y

L

M

N

E

D

Q

Q

G

E

R

L

L

K

S

V

V

Q

A

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D
|
D

T

T

G

G

T

T

H

H

D

Q

S

S

L

L

S

I

E

E

A

A

S

S

T

N

F

F

I

I

E

A

V

T

I

I

E

E

K

E

R

R

Q

Q

G

G

L

L

K

K

V

V

A

S

C

C

L

P

E

E

G

E

I

I

A

A

L

F

R

R

H

K

G

N

W

F

I

I

T

N

T

A

D

Q

K

Q

M

V

R

I

E

E

L

L

A

A

Q

G

P

P

M

L

L

S

K

K

N

N

Q

D

Y

Y

G

G

Q

K

Y

Y

L

L

K

K

V

M

I

V

binding 2'-deoxyguanosine-5'-triphosphate: L7 (= L6), G9 (= G8), G10 (= G9), S11 (= S10), G12 (= G11), T13 (= T12), R14 (= R13), Q25 (= Q24), D81 (≠ Q80), S83 (= S82), L107 (= L106), G108 (= G107), D109 (= D108)
binding magnesium ion: D109 (= D108), D221 (= D226)

5fu8A Pseudomonas aeruginosa rmla in complex with allosteric inhibitor
62% identity, 98% coverage: 2:291/295 of query aligns to 8:294/297 of 5fu8A

query
sites
5fu8A

K

K

G

G

I

I

V

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

Y

H

P

P

I

A

T

T

K

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

I

V

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S
|
S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

E

E

I

I

L

L

I

I

S

S

T

T

P

P

Y

Q

D

D

L

T

P

P

G

R

F

F

K

Q

R

Q

L

L

G

G

D

D

G

G

S

S

D

N

Y

W

G

G

V

L

H

D

F

L

E

Q

Y

Y

A

A

E

V

Q

Q

P

P

S

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

E

S

F

F

I

I

G

G

N

N

D

D

S

L

V

S

C

A

L

L

V

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

Y
|
Y

G
|
G

Q

H

G

D

F

F

T
x
H

H

E

M

L

L

L

K

G

E

S

A

A

V

-

H

-

T

-

A

S

E

Q

E

R

E

Q

N

T

K

G

A

A

T

S

V

V

F

F

G

A

Y

Y

W

H

V

V

A

L

D

D

P

P

E

E

R

R

Y

Y

G

G

V

V

A

V

E

E

F

F

D

D

K

Q

I

G

G

G

N

K

V

A

L

I

S

S

I

L

E

E

K

K

P

P

I

L

Q

E

P

P

K

K

S

S

N

N

Y

Y

A

A

V

V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

Q

K

Q

V

V

E

D

V

I

A

A

K

R

N

D

I

L

T

K

P

P

S

S

S

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

A

D

V

V

N

N

Q

R

Q

A

F

Y

L

L

N

E

D

R

Q

G

E

Q

L

L

K

S

V

V

Q

E

L

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

S

L

E

E

A

A

S

G

T

Q

F

F

I

I

E

A

V

T

I

L

E

E

K

N

R

R

Q

Q

G

G

L

L

K

K

V
|
V

A

A

C

C

L

P

E

E

G
x
E

I

I

A

A

L

Y

R
|
R

H
x
Q

G

K

W

W

I

I

T

D

T

A

D

A

K

Q

M

L

R

E

E

K

L

L

A

A

Q

A

P

P

M

L

L

A

K

K

N

N

Q

G

Y

Y

G

G

Q

Q

Y

Y

L

L

L

K

K

R

V

L

I

L

D

T

E

E

binding N-(6-amino-1-(4-bromo-3-methylbenzyl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbenzenesulfonamide: S45 (= S39), L49 (= L43), Y117 (≠ F111), Y118 (= Y112), G119 (= G113), H123 (≠ T117), V254 (= V251), E259 (≠ G256), R263 (= R260), Q264 (≠ H261)

4b2wA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
62% identity, 98% coverage: 2:291/295 of query aligns to 8:294/297 of 4b2wA

query
sites
4b2wA

K

K

G

G

I

I

V

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

Y

H

P

P

I

A

T

T

K

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

I

V

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S
|
S

V

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

E

E

I

I

L

L

I

I

S

S

T

T

P

P

Y

Q

D

D

L

T

P

P

G

R

F

F

K

Q

R

Q

L

L

G

G

D

D

G

G

S

S

D

N

Y

W

G

G

V

L

H

D

F

L

E

Q

Y

Y

A

A

E

V

Q

Q

P

P

S

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

E

S

F

F

I

I

G

G

N

N

D

D

S

L

V

S

C

A

L

L

V

V

L

L

G

G

D

D

N

N

I

L

F

Y

Y

Y

G

G

Q

H

G

D

F

F

T

H

H

E

M

L

L

L

K

G

E

S

A

A

V

-

H

-

T

-

A

S

E

Q

E

R

E

Q

N

T

K

G

A

A

T

S

V

V

F

F

G

A

Y

Y

W

H

V

V

A

L

D

D

P

P

E

E

R

R

Y

Y

G

G

V

V

A

V

E

E

F

F

D

D

K

Q

I

G

G

G

N

K

V

A

L

I

S

S

I

L

E

E

K

K

P

P

I

L

Q

E

P

P

K

K

S

S

N

N

Y

Y

A

A

V

V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

Q

K

Q

V

V

E

D

V

I

A

A

K

R

N

D

I

L

T

K

P

P

S

S

S

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

A

D

V

V

N

N

Q

R

Q

A

F

Y

L

L

N

E

D

R

Q

G

E

Q

L

L

K

S

V

V

Q

E

L

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

S

L

E

E

A

A

S

G

T

Q

F

F

I

I

E

A

V

T

I

L

E

E

K

N

R

R

Q

Q

G

G

L

L

K

K

V

V

A

A

C

C

L

P

E

E

G
x
E

I
|
I

A

A

L

Y

R
|
R

H

Q

G

K

W

W

I

I

T

D

T

A

D

A

K

Q

M

L

R

E

E

K

L

L

A

A

Q

A

P

P

M

L

L

A

K

K

N

N

Q

G

Y

Y

G

G

Q

Q

Y

Y

L

L

L

K

K

R

V

L

I

L

D

T

E

E

binding N-(6-amino-1-benzyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)benzamide: S45 (= S39), L49 (= L43), E259 (≠ G256), I260 (= I257), R263 (= R260)

5fuhA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor
62% identity, 98% coverage: 2:291/295 of query aligns to 9:295/298 of 5fuhA

query
sites
5fuhA

K

K

G

G

I

I

V

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

Y

H

P

P

I

A

T

T

K

L

G

A

V

I

S

S

K

K

Q

Q

L

L

P

P

I

V

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

I

L

S

S

V
x
T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

E

E

I

I

L

L

I

I

S

S

T

T

P

P

Y

Q

D

D

L

T

P

P

G

R

F

F

K

Q

R

Q

L

L

G

G

D

D

G

G

S

S

D

N

Y

W

G

G

V

L

H

D

F

L

E

Q

Y

Y

A

A

E

V

Q

Q

P

P

S

S

P

P

D

D

G

G

L

L

A

A

Q

Q

A

A

F

F

I

L

I

I

G

G

E

E

E

S

F

F

I

I

G

G

N

N

D

D

S

L

V

S

C

A

L

L

V

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

Y

Y

G
|
G

Q

H

G

D

F
|
F

T

H

H

E

M

L

L

L

K

G

E

S

A

A

V

-

H

-

T

-

A

S

E

Q

E

R

E

Q

N

T

K

G

A

A

T

S

V

V

F

F

G

A

Y

Y

W

H

V

V

A

L

D

D

P

P

E

E

R

R

Y

Y

G

G

V

V

A

V

E

E

F

F

D

D

K

Q

I

G

G

G

N

K

V

A

L

I

S

S

I

L

E

E

K

K

P

P

I

L

Q

E

P

P

K

K

S

S

N

N

Y

Y

A

A

V

V

V

T

G

G

L

L

Y

Y

F

F

Y

Y

P

D

N

Q

K

Q

V

V

E

D

V

I

A

A

K

R

N

D

I

L

T

K

P

P

S

S

S

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

A

D

V

V

N

N

Q

R

Q

A

F

Y

L

L

N

E

D

R

Q

G

E

Q

L

L

K

S

V

V

Q

E

L

I

L

M

G

G

R

R

G

G

F

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

D

D

S

S

L

L

S

L

E

E

A

A

S

G

T

Q

F

F

I

I

E

A

V

T

I

L

E

E

K

N

R

R

Q

Q

G

G

L

L

K

K

V

V

A
|
A

C

C

L

P

E

E

G

E

I

I

A

A

L

Y

R
|
R

H
x
Q

G

K

W

W

I

I

T

D

T

A

D

A

K

Q

M

L

R

E

E

K

L

L

A

A

Q

A

P

P

M

L

L

A

K

K

N

N

Q

G

Y

Y

G

G

Q

Q

Y

Y

L

L

L

K

K

R

V

L

I

L

D

T

E

E

binding N-[6-amino-1-(3-bromobenzyl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl]-N-methylbenzenesulfonamide: T47 (≠ V40), L50 (= L43), Y118 (≠ F111), G120 (= G113), F123 (= F116), A256 (= A252), R264 (= R260), Q265 (≠ H261)

Query Sequence

>349991 FitnessBrowser__Btheta:349991
MKGIVLAGGSGTRLYPITKGVSKQLLPIFDKPMIYYPISVLMLAGIREILIISTPYDLPG
FKRLLGDGSDYGVHFEYAEQPSPDGLAQAFIIGEEFIGNDSVCLVLGDNIFYGQGFTHML
KEAVHTAEEENKATVFGYWVADPERYGVAEFDKIGNVLSIEEKPIQPKSNYAVVGLYFYP
NKVVEVAKNITPSSRGELEITAVNQQFLNDQELKVQLLGRGFAWLDTGTHDSLSEASTFI
EVIEKRQGLKVACLEGIALRHGWITTDKMRELAQPMLKNQYGQYLLKVIDELTQK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory