SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 350049 FitnessBrowser__Btheta:350049 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3nz2J Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
38% identity, 52% coverage: 89:195/207 of query aligns to 77:183/185 of 3nz2J

query
sites
3nz2J

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binding acetyl coenzyme *a: G104 (= G121), Y110 (≠ Q127), H114 (≠ K131), W138 (= W150), G140 (= G152), A158 (= A170), A159 (= A171), N164 (≠ S176), G174 (≠ A186), T176 (≠ C188), P177 (= P189), R182 (≠ K194)

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
38% identity, 52% coverage: 89:195/207 of query aligns to 74:180/183 of 3nz2C

query
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3nz2C

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binding acetyl coenzyme *a: Y107 (≠ Q127), H111 (≠ K131), G137 (= G152), A155 (= A170), A156 (= A171), N161 (≠ S176), G171 (≠ A186), T173 (≠ C188), P174 (= P189), L178 (≠ I193), R179 (≠ K194)
binding magnesium ion: N157 (≠ G172), T173 (≠ C188)

3ectA Crystal structure of the hexapeptide-repeat containing- acetyltransferase vca0836 from vibrio cholerae
38% identity, 52% coverage: 89:195/207 of query aligns to 67:173/176 of 3ectA

query
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3ectA

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binding calcium ion: G138 (= G160), D156 (≠ S178)

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
34% identity, 56% coverage: 88:203/207 of query aligns to 90:192/203 of P07464

query
sites
P07464

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D93 (= D91) binding
H115 (= H124) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A153) binding in other chain
A160 (= A171) binding in other chain
TK 165:166 (≠ SR 176:177) binding
R180 (≠ K191) binding
R183 (≠ K194) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding
71 binding
85 binding in other chain; binding in other chain

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
34% identity, 56% coverage: 88:203/207 of query aligns to 89:191/200 of 1krrA

query
sites
1krrA

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binding acetyl coenzyme *a: I103 (≠ L113), A104 (= A114), P105 (≠ Q115), T112 (≠ L122), H114 (= H124), G140 (= G152), S141 (≠ A153), N146 (≠ L158), G158 (≠ A170), A159 (= A171), A174 (= A186), P177 (= P189), R182 (≠ K194)

Sites not aligning to the query:

binding acetyl coenzyme *a: 84

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
34% identity, 56% coverage: 88:203/207 of query aligns to 89:191/201 of 1krvA

query
sites
1krvA

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binding 4-nitrophenyl beta-D-galactopyranoside: V90 (≠ I89), D92 (= D91), M126 (≠ V138)
binding coenzyme a: A104 (= A114), S110 (≠ T120), T112 (≠ L122), W138 (= W150), G140 (= G152), S141 (≠ A153), N146 (≠ L158), A159 (= A171), P177 (= P189), R179 (≠ K191), R182 (≠ K194)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25, 70, 82, 84

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
34% identity, 56% coverage: 88:203/207 of query aligns to 89:191/201 of 1kruA

query
sites
1kruA

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binding coenzyme a: H114 (= H124), W138 (= W150), G140 (= G152), S141 (≠ A153), G158 (≠ A170), A159 (= A171), P177 (= P189), R182 (≠ K194)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: V90 (≠ I89), D92 (= D91), L102 (≠ N112), H114 (= H124), M126 (≠ V138)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25, 62, 70, 82, 82, 84

3igjC Crystal structure of maltose o-acetyltransferase complexed with acetyl coenzyme a from bacillus anthracis
31% identity, 62% coverage: 69:197/207 of query aligns to 61:187/188 of 3igjC

query
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3igjC

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binding acetyl coenzyme *a: F84 (≠ I89), A106 (= A114), Y112 (≠ T120), A114 (≠ L122), H116 (= H124), G142 (= G152), N148 (≠ L158), A160 (= A170), S161 (≠ A171), T166 (≠ S176), N178 (≠ C188)

4isxA The crystal structure of maltose o-acetyltransferase from clostridium difficile 630 in complex with acetyl-coa
36% identity, 43% coverage: 105:194/207 of query aligns to 95:182/186 of 4isxA

query
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binding acetyl coenzyme *a: A104 (= A114), W138 (= W150), G140 (= G152), G141 (≠ A153), A159 (= A171), V174 (≠ A186), K182 (= K194)

Sites not aligning to the query:

binding acetyl coenzyme *a: 84

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
33% identity, 46% coverage: 103:197/207 of query aligns to 93:185/190 of 5u2kA

query
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5u2kA

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F

L

I

A

G

Q

P

N

N

I

C

T

G

V

F

T
x
Y

G

T

L
x
A

N

T

H
|
H

-

P

-

L

N

N

Y

F

Q

H

D

H

A

R

E

N

K

E

S

G

I

F

D

E

E

K

Q

A

G

G

V

-

S

-

T

-

Q

-

P

P

V

I

T

H

I

I

E

G

D

S

D

N

V

T

W

W

V

F

G

G

A

G

N

H

S

V

V

A

I

V

L
|
L

P

P

G

G

V

V

T

T

L

I

G

G

K

E

H

G

C

S

V

V

V

I

A

G

A

A

G

G

S

S

V

V

S
x
T

R
x
K

S

D

I

I

P

P

A

P

Y

H

S

S

I

L

C

A

A

V

G

G

C

N

P

P

A

C

K

K

V

V

I

V

K

R

S

K

Y

I

D

D

binding coenzyme a: Y110 (≠ T120), A112 (≠ L122), H114 (= H124), L146 (= L158), T164 (≠ S176), K165 (≠ R177)

Sites not aligning to the query:

binding coenzyme a: 90

4hurA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with acetyl coenzyme a (see paper)
31% identity, 58% coverage: 84:203/207 of query aligns to 56:175/211 of 4hurA

query
sites
4hurA

V

I

G

G

D

D

-

K

L

L

I

I

G

G

D

R

Y

F

T

C

R
x
S

I
|
I

G
|
G

L

P

G

G

N

T

T

T

I

F

I

I

G

-

P

-

V

M

R

N

I

G

G
x
A

N
|
N

H

H

V

R

N

M

L

D

A

G

Q

S

N

T

I

Y

T

P

V

F

T

H

G

L

L

F

N

R

H

M

N

G

Y

W

Q

E

D

K

A

Y

E

M

K

P

S

S

I

L

D

K

E

D

Q

L

G

P

V

L

S
x
K

T

G

Q

D

P

-

V

I

T

E

I

I

E

G

D

N

D

D

V

V

W
|
W

V
x
I

G
|
G

A
x
R

N

D

S

V

V

T

I

I

L
x
M

P

P

G

G

V

V

T

K

L

I

G

G

K

D

H

G

C

A

V

I

A
|
A

G

E

S

A

V

V

S
x
T

R

K

S

N

I

V

P

A

A

P

Y

Y

S

S

I

I

C

V

A
x
G

G

G

C

N

P
|
P

A

L

K

K

V

F

I
|
I

K
x
R

S

K

-

R

-

F

Y

S

D

D

F

G

A

V

T

I

K

E

E

E

W

W

binding acetyl coenzyme *a: S67 (≠ R94), I68 (= I95), G69 (= G96), A79 (≠ G108), N80 (= N109), K110 (≠ S139), W120 (= W150), I121 (≠ V151), G122 (= G152), R123 (≠ A153), M128 (≠ L158), A140 (= A170), A141 (= A171), T146 (≠ S176), G156 (≠ A186), P159 (= P189), I163 (= I193), R164 (≠ K194)

Sites not aligning to the query:

binding acetyl coenzyme *a: 51

6x3cA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
31% identity, 58% coverage: 84:203/207 of query aligns to 56:175/207 of 6x3cA

query
sites
6x3cA

V

I

G

G

D

D

-

K

L

L

I

I

G

G

D

R

Y

F

T

C

R

S

I

I

G
|
G

L

P

G

G

N

T

T

T

I

F

I

I

G

-

P

-

V

M

R

N

I
x
G

G

A

N
|
N

H
|
H

V

R

N

M

L

D

A

G

Q

S

N

T

I

Y

T

P

V

F

T
x
H

G
x
L

L

F

N

R

H

M

N

G

Y

W

Q

E

D

K

A

Y

E
x
M

K
x
P

S

S

I
x
L

D

K

E

D

Q

L

G

P

V

L

S

K

T

G

Q

D

P

-

V

I

T

E

I

I

E

G

D

N

D

D

V

V

W
|
W

V

I

G
|
G

A
x
R

N

D

S

V

V

T

I

I

L
x
M

P

P

G

G

V

V

T

K

L

I

G

G

K

D

H

G

C

A

V

I

A
|
A

G

E

S

A

V

V

S

T

R

K

S

N

I

V

P

A

A

P

Y

Y

S

S

I

I

C

V

A

G

G

G

C

N

P

P

A

L

K

K

V

F

I

I

K

R

S

K

-

R

-

F

Y

S

D

D

F

G

A

V

T

I

K

E

E

E

W

W

binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: G78 (≠ I107), N80 (= N109), H81 (= H110), H91 (≠ T120), L92 (≠ G121), M101 (≠ E130), P102 (≠ K131), L104 (≠ I133)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (= G96), N80 (= N109), H81 (= H110), W120 (= W150), G122 (= G152), R123 (≠ A153), M128 (≠ L158), A140 (= A170), A141 (= A171)

Sites not aligning to the query:

4husA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with virginiamycin m1 (see paper)
31% identity, 58% coverage: 84:203/207 of query aligns to 56:175/212 of 4husA

query
sites
4husA

V

I

G

G

D

D

-

K

L

L

I

I

G

G

D

R

Y

F

T

C

R

S

I

I

G

G

L

P

G

G

N

T

T

T

I

F

I

I

G

-

P

-

V

M

R

N

I

G

G

A

N

N

H

H

V

R

N

M

L

D

A

G

Q

S

N

T

I

Y

T

P

V

F

T

H

G

L

L

F

N

R

H

M

N

G

Y

W

Q

E

D

K

A

Y

E

M

K

P

S

S

I

L

D

K

E

D

Q

L

G

P

V

L

S

K

T

G

Q

D

P

-

V

I

T

E

I

I

E

G

D

N

D

D

V

V

W

W

V

I

G

G

A

R

N

D

S

V

V

T

I

I

L

M

P

P

G

G

V

V

T

K

L

I

G

G

K

D

H

G

C

A

V

I

A

A

G

E

S

A

V

V

S

T

R

K

S

N

I

V

P

A

A

P

Y

Y

S

S

I

I

C

V

A

G

G

G

C

N

P

P

A

L

K

K

V

F

I

I

K

R

S

K

-

R

-

F

Y

S

D

D

F

G

A

V

T

I

K

E

E

E

W

W

Sites not aligning to the query:

binding virginiamycin m1: 17, 36, 38

6x3jA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f0224 (46) (see paper)
31% identity, 58% coverage: 84:203/207 of query aligns to 56:175/206 of 6x3jA

query
sites
6x3jA

V

I

G

G

D

D

-

K

L

L

I

I

G

G

D

R

Y

F

T

C

R

S

I

I

G
|
G

L

P

G

G

N

T

T

T

I

F

I

I

G

-

P

-

V

M

R

N

I

G

G
x
A

N

N

H
|
H

V

R

N

M

L

D

A

G

Q

S

N

T

I

Y

T

P

V

F

T
x
H

G
x
L

L

F

N

R

H

M

N

G

Y

W

Q

E

D

K

A

Y

E
x
M

K
x
P

S

S

I

L

D

K

E

D

Q
x
L

G

P

V

L

S

K

T

G

Q

D

P

-

V

I

T

E

I

I

E

G

D

N

D

D

V

V

W
|
W

V

I

G
|
G

A

R

N

D

S

V

V

T

I

I

L

M

P

P

G

G

V

V

T

K

L

I

G

G

K

D

H

G

C

A

V

I

A

A

G

E

S

A

V

V

S

T

R

K

S

N

I

V

P

A

A

P

Y

Y

S

S

I

I

C

V

A

G

G

G

C

N

P

P

A

L

K

K

V

F

I

I

K

R

S

K

-

R

-

F

Y

S

D

D

F

G

A

V

T

I

K

E

E

E

W

W

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: H91 (≠ T120), L92 (≠ G121), M101 (≠ E130), P102 (≠ K131), L107 (≠ Q136)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (= G96), A79 (≠ G108), H81 (= H110), W120 (= W150), G122 (= G152)

Sites not aligning to the query:

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: 51, 53, 55

6x3cE Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
31% identity, 58% coverage: 84:203/207 of query aligns to 56:175/203 of 6x3cE

query
sites
6x3cE

V

I

G

G

D

D

-

K

L

L

I

I

G

G

D

R

Y

F

T

C

R

S

I

I

G
|
G

L

P

G

G

N

T

T

T

I

F

I

I

G

-

P

-

V

M

R

N

I
x
G

G

A

N
|
N

H
|
H

V

R

N
x
M

L

D

A

G

Q

S

N

T

I

Y

T

P

V

F

T
x
H

G
x
L

L

F

N

R

H

M

N

G

Y

W

Q

E

D

K

A

Y

E
x
M

K
x
P

S

S

I

L

D

K

E

D

Q

L

G

P

V

L

S

K

T

G

Q

D

P

-

V

I

T

E

I

I

E

G

D

N

D

D

V

V

W
|
W

V

I

G
|
G

A

R

N

D

S

V

V

T

I

I

L

M

P

P

G

G

V

V

T

K

L

I

G

G

K

D

H

G

C

A

V

I

A
|
A

G

E

S

A

V

V

S

T

R

K

S

N

I

V

P

A

A

P

Y

Y

S

S

I

I

C

V

A

G

G

G

C
x
N

P
|
P

A

L

K

K

V

F

I

I

K

R

S

K

-

R

-

F

Y

S

D

D

F

G

A

V

T

I

K

E

E

E

W

W

binding magnesium ion: N158 (≠ C188), P159 (= P189)
binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: G78 (≠ I107), N80 (= N109), H81 (= H110), H91 (≠ T120), L92 (≠ G121), M101 (≠ E130), P102 (≠ K131)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (= G96), H81 (= H110), M83 (≠ N112), W120 (= W150), G122 (= G152), A140 (= A170), A141 (= A171)

Sites not aligning to the query:

P50870 Streptogramin A acetyltransferase; Virginiamycin acetyltransferase D; Vat(D); EC 2.3.1.- from Enterococcus faecium (Streptococcus faecium) (see paper)
33% identity, 53% coverage: 87:195/207 of query aligns to 61:166/209 of P50870

query
sites
P50870

L

L

I

K

I

I

G

G

D

K

Y

F

T

C

R

S

I

I

G

G

L

P

G

G

N

V

T

T

I

I

G

-

P

-

V

M

R

N

I

G

G

A

N

N

H
|
H

V

R

N

M

L

D

A

G

Q

S

N

T

I

Y

T

P

V

F

T

N

G

L

L

F

N

G

H

N

N

G

Y

W

Q

E

D

K

A

H

E

M

K

P

S

K

I

L

D

D

E

Q

Q

L

G

P

V

I

S

K

T

G

Q

D

P

T

V

I

T

-

I

I

E

G

D

N

D

D

V

V

W

W

V

I

G

G

A

K

N

D

S

V

V

V

I

I

L

M

P

P

G

G

V

V

T

K

L

I

G

G

K

D

H

G

C

A

V

I

V

V

A

A

G

N

S

S

V

V

S

V

R

K

S

D

I

I

P

A

A

P

Y

Y

S

M

I

L

C

A

A

G

G

G

C

N

P

P

A

A

K

N

V

E

I

I

K

K

S

Q

H82 (= H110) mutation to A: 105-fold decrease in activity.

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
31% identity, 55% coverage: 79:192/207 of query aligns to 38:139/290 of 4mzuB

query
sites
4mzuB

C
|
C

L
x
A

N

N

N

S

A

L

V

I

-

E

G

N

D

D

L

V

I

V

I

I

G

G

D

D

Y

N

T

V

R

T

I

I

G

K

L

S

G
|
G

N

V

T

Q

I

I

I

W

G

D

P

G

V

I

R

H

I

I

G

Q

N

D

H

D

V

V

N
x
F

L

I

A

G

Q

P

N
|
N

I

V

T

T

V

F

T
|
T

G

-

L

-

N
|
N

H
x
D

N
x
K

Y

Q

Q
x
P

D

R

A

S

E

L

K

K

S

T

I

I

D

V

E

K

Q

K

G

G

V

A

S

S

T

-

Q

-

P

-

V

-

T

-

I

-

E

-

D

-

V

-

W

-

V

I

G
|
G

A
|
A

N

N

S

S

V

T

I

I

L

L

P

P

G

G

V

I

T

L

L

I

G

G

K

E

H

N

C

A

V

M

V

V

A
x
G

A
|
A

G

G

S

A

V

V

V

I

S
x
T

R
x
K

S

N

I

V

P

P

A

D

Y

N

S

A

I

I

C

V

A
x
I

G

G

C

N

P

P

A

G

K
x
R

V

I

binding coenzyme a: F72 (≠ N112), T80 (= T120), N81 (= N123), D82 (≠ H124), K83 (≠ N125), P85 (≠ Q127), G99 (= G152), A100 (= A153), G117 (≠ A170), A118 (= A171), T123 (≠ S176), K124 (≠ R177), I133 (≠ A186), R138 (≠ K191)
binding magnesium ion: G58 (= G98), N76 (= N116)
binding thymine: C38 (= C79), A39 (≠ L80)

Sites not aligning to the query:

binding coenzyme a: 199
binding thymine: 20, 21
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

3dhoA Structure of streptogramin acetyltransferase in complex with an inhibitor
33% identity, 53% coverage: 87:195/207 of query aligns to 61:166/203 of 3dhoA

query
sites
3dhoA

L

L

I

K

I

I

G

G

D

K

Y

F

T

C

R

S

I

I

G

G

L

P

G

G

N

V

T

T

I

I

G

-

P

-

V

M

R

N

I

G

G

A

N

N

H

H

V

R

N
x
M

L

D

A

G

Q

S

N

T

I

Y

T

P

V

F

T

N

G

L

L

F

N

G

H

N

N

G

Y

W

Q

E

D

K

A

H

E

M

K

P

S

K

I

L

D

D

E

Q

Q

L

G

P

V

I

S

K

T

G

Q

D

P

T

V

I

T

-

I

I

E

G

D

N

D

D

V

V

W

W

V

I

G

G

A

K

N

D

S

V

V

V

I

I

L

M

P

P

G

G

V

V

T

K

L

I

G

G

K

D

H

G

C

A

V
x
I

V

V

A
|
A

A

A

G

N

S

S

V

V

S
x
V

R

K

S

D

I

I

P

A

A

P

Y

Y

S

M

I
x
L

C

A

A
x
G

G

G

C

N

P
|
P

A

A

K

N

V

E

I
|
I

K

K

S

Q

binding 6-bromo-N'-[(1Z)-(3,5-dichloro-2-hydroxyphenyl)methylidene]-2-methylquinoline-4-carbohydrazide: M84 (≠ N112), I139 (≠ V168), A141 (= A170), V147 (≠ S176), L155 (≠ I184), G157 (≠ A186), P160 (= P189), I164 (= I193)

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
30% identity, 55% coverage: 79:192/207 of query aligns to 38:140/294 of 4mzuF

query
sites
4mzuF

C
|
C

L

A

N

N

N

S

A

L

V

I

-

E

G

N

D

D

L

V

I

V

I

I

G

G

D

D

Y

N

T

V

R

T

I

I

G

K

L

S

G

G

N

V

T

Q

I

I

I

W

G

D

P

G

V

I

R

H

I

I

G

Q

N

D

H

D

V

V

N

F

L

I

A

G

Q

P

N

N

I

V

T

T

V

F

T

T

G
x
N

L

-

N

-

H

-

N

-

Y

-

Q

-

D

-

A

-

E

-

K

-

S

-

I

-

D
|
D

E
x
K

Q

Q

G

P

V

R

S

S

T

K

Q

I

P

K

V

T

T

I

I

V

E

K

D

K

D

G

V

A

W

S

V

I

G
|
G

A
|
A

N

N

S

S

V

T

I

I

L
|
L

P

P

G

G

V

I

T

L

L

I

G

G

K

E

H

N

C

A

V

M

V

V

A
x
G

A
|
A

G

G

S

A

V

V

V

I

S
x
T

R
x
K

S

N

I

V

P

P

A

D

Y

N

S

A

I

I

C

V

A
x
I

G

G

C
x
N

P

P

A

G

K
x
R

V

I

binding coenzyme a: N81 (≠ G121), D82 (= D134), K83 (≠ E135), G100 (= G152), A101 (= A153), L106 (= L158), G118 (≠ A170), A119 (= A171), T124 (≠ S176), K125 (≠ R177), I134 (≠ A186), N136 (≠ C188), R139 (≠ K191)
binding thymine: C38 (= C79)

Sites not aligning to the query:

1mrlA Crystal structure of streptogramin a acetyltransferase with dalfopristin (see paper)
33% identity, 53% coverage: 87:195/207 of query aligns to 61:166/204 of 1mrlA

query
sites
1mrlA

L

L

I

K

I

I

G

G

D

K

Y

F

T

C

R

S

I

I

G

G

L

P

G

G

N

V

T

T

I

I

G

-

P

-

V

M

R

N

I

G

G

A

N
|
N

H
|
H

V

R

N

M

L

D

A

G

Q

S

N

T

I

Y

T

P

V

F

T

N

G
x
L

L

F

N

G

H

N

N

G

Y

W

Q

E

D

K

A

H

E
x
M

K

P

S

K

I

L

D

D

E

Q

Q
x
L

G

P

V

I

S

K

T

G

Q

D

P

T

V

I

T

-

I

I

E

G

D

N

D

D

V

V

W

W

V

I

G

G

A

K

N

D

S

V

V

V

I

I

L

M

P

P

G

G

V

V

T

K

L

I

G

G

K

D

H

G

C

A

V

I

V

V

A

A

G

N

S

S

V

V

S

V

R

K

S

D

I

I

P

A

A

P

Y

Y

S

M

I

L

C

A

A

G

G

G

C

N

P

P

A

A

K

N

V

E

I

I

K

K

S

Q

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: N81 (= N109), H82 (= H110), L93 (≠ G121), M102 (≠ E130), L108 (≠ Q136)

Sites not aligning to the query:

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: 37, 39, 54, 56

Query Sequence

>350049 FitnessBrowser__Btheta:350049
MEKRTLKQRIKQNPALKQAVHRFIMHPVKTRPNWWIRLFYFVYLKRGKGSVIYRSVRKDL
PPFNQFSLGRYSVVEDFSCLNNAVGDLIIGDYTRIGLGNTIIGPVRIGNHVNLAQNITVT
GLNHNYQDAEKSIDEQGVSTQPVTIEDDVWVGANSVILPGVTLGKHCVVAAGSVVSRSIP
AYSICAGCPAKVIKSYDFATKEWKKVK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory