SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 350176 FitnessBrowser__Btheta:350176 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
50% identity, 95% coverage: 2:219/230 of query aligns to 10:232/271 of Q72J02

query
sites
Q72J02

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C192 (vs. gap) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.

Sites not aligning to the query:

268 C→S: Still able to form a thioester complex with TtuB.

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
42% identity, 95% coverage: 2:219/230 of query aligns to 71:288/453 of Q9VLJ8

query
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Q9VLJ8

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Sites not aligning to the query:

62 modified: Phosphothreonine

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 5 papers)
44% identity, 95% coverage: 1:219/230 of query aligns to 61:279/460 of O95396

query
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O95396

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158:238 (vs. 98:186, 36% identical) Interaction with NFS1
C239 (≠ I187) mutation to A: Impairs sulfurtransferase activity.

Sites not aligning to the query:

316 modified: Disulfide link with 324; C→A: Does not affect sulfurtransferase activity.
324 modified: Disulfide link with 316; C→A: Does not affect sulfurtransferase activity.
365 C→A: Does not affect sulfurtransferase activity.
412 active site, Cysteine persulfide intermediate; for sulfurtransferase activity; modified: Cysteine persulfide; C→A: Abolishes sulfurtransferase activity.
413 K→R: Does not affect sulfurtransferase specificity and activity.
414 L→K: Does not affect sulfurtransferase specificity and activity.
415 G→A: Does not affect sulfurtransferase specificity and activity.
416 N→V: Does not affect sulfurtransferase specificity and activity.
417 D→R: Results in 470-fold increased activity.; D→T: Results in 90-fold increased activity.
458 P→G: Does not affect sulfurtransferase specificity and activity.
460 Y→A: Does not affect sulfurtransferase specificity and activity.

1zud3 Structure of this-thif protein complex (see paper)
40% identity, 100% coverage: 1:230/230 of query aligns to 7:229/240 of 1zud3

query
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1zud3

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active site: R11 (= R5), D127 (= D121)
binding calcium ion: L14 (= L8), D16 (≠ P10)
binding zinc ion: C169 (≠ S163)

Sites not aligning to the query:

binding zinc ion: 235, 238

1zfnA Structural analysis of escherichia coli thif (see paper)
40% identity, 100% coverage: 1:230/230 of query aligns to 7:234/244 of 1zfnA

query
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1zfnA

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active site: R11 (= R5), D127 (= D121)
binding adenosine-5'-triphosphate: I34 (≠ V28), G35 (= G29), G37 (= G31), G38 (= G32), D59 (= D53), R70 (= R64), Q71 (= Q65), K83 (= K77), T126 (≠ C120), D127 (= D121), T131 (= T125)
binding zinc ion: C169 (≠ S163), C172 (≠ D166)

Sites not aligning to the query:

binding zinc ion: 240, 243

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
40% identity, 100% coverage: 1:230/230 of query aligns to 7:234/251 of P30138

query
sites
P30138

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C169 (≠ S163) binding
C172 (≠ D166) binding
W174 (≠ Y168) mutation to A: No adenylation of ThiS.
C184 (≠ P178) mutation to S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.

Sites not aligning to the query:

240 binding
243 binding

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
38% identity, 98% coverage: 2:226/230 of query aligns to 68:314/482 of O59954

query
sites
O59954

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G82 (= G16) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (= G34) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R64) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (≠ G119) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.
E215 (= E149) mutation to K: In cnxF119; impairs molybdopterin biosynthesis.
G264 (≠ A190) mutation to S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
42% identity, 89% coverage: 2:206/230 of query aligns to 46:252/440 of P38820

query
sites
P38820

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C225 (vs. gap) mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.

Sites not aligning to the query:

397 mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
38% identity, 100% coverage: 1:230/230 of query aligns to 10:238/249 of P12282

query
sites
P12282

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R14 (= R5) mutation R->A,K: No effect.; mutation to A: No activity; when associated with A-73.
C44 (≠ S35) mutation to A: No effect.
R73 (= R64) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (≠ G119) mutation to A: No effect.; mutation to Y: No activity.
D130 (= D121) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (= C133) mutation to A: No effect.
C172 (≠ S163) mutation to A: No zinc bound and no enzyme activity.
C175 (≠ D166) mutation to A: No zinc bound and no enzyme activity.
C187 (≠ P179) mutation to A: No effect.
C231 (≠ L223) mutation to A: No effect.

Sites not aligning to the query:

244 C→A: No zinc bound and almost no enzyme activity.
247 C→A: No zinc bound and almost no enzyme activity.

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
38% identity, 100% coverage: 1:230/230 of query aligns to 9:230/240 of 1jwbB

query
sites
1jwbB

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active site: R13 (= R5), D129 (= D121)
binding adenosine monophosphate: G37 (= G29), G39 (= G31), G40 (= G32), D61 (= D53), F62 (≠ D54), K85 (= K77), L108 (≠ R100), C127 (≠ G119), T128 (≠ C120), D129 (= D121), N130 (= N122), V133 (≠ T125)
binding zinc ion: C171 (≠ S163)

Sites not aligning to the query:

binding zinc ion: 236, 239

1jw9B Structure of the native moeb-moad protein complex (see paper)
38% identity, 100% coverage: 1:230/230 of query aligns to 9:230/240 of 1jw9B

query
sites
1jw9B

M

L

R

R

Y

Y

D

N

R
|
R

Q

Q

M

I

L

I

L

L

P

R

E

G

I

F

G

D

E

F

D

D

G

G

Q

Q

Q

E

K

A

L

L

K

K

Q

D

A

S

K

R

V

V

L

L

I

I

V

V

G

G

V

L

G

G

L

L

G

G

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C

P

A

I

A

A

S

L

Q

Y

Y

L

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A

G

S

A

A

G

G

V

V

G

G

C

N

I

L

G

T

L

L

V

L

D

D

D

F

D

D

V

T

V

V

S

S

I

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

T

L

L

Y

H

S

S

E

D

K

A

E

T

L

V

G

G

K

Q

P

P

K

K

A

V

I

E

C

S

A

A

A

R

E

D

R

A

L

L

S

T

A

R

L

I

N

N

S

P

E

H

I

I

T

A

I

I

R

T

T

P

Y

V

P

N

T

A

R

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L

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T

D

E

D

E

A

N

E

A

L

Q

A

E

A

I

L

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A

Q

E

Y

H

D

D

I

L

V

V

V

L

D

D

G

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C

T

D
|
D

N

N

F

V

S

A

T

V

R

R

Y

N

L

Q

I

L

N

N

D

A

I

G

C

C

A

F

E

A

M

A

G

K

K

V

V

P

Y

L

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V

Y

S

G

G

A

A

I

A

C

I

G

R

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M

E

E

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G

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Q

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T

V

V

F

F

H

T

Y

Y

G

Q

E

D

E

G

K

E

K

P

S
x
C

Y

Y

R

R

D

C

L

L

Y

S

P

R

D

L

E

F

E

G

E

E

M

-

R

-

M

-

P

-

K

A

G

G

V

V

M

M

G

A

I

P

T

L

P

I

A

G

V

V

T

I

G

G

S

S

I

L

E

Q

A

A

T

M

E

E

V

A

L

I

K

K

I

M

I

L

C

A

G

G

F

Y

G

G

E

K

V

P

L

A

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S

G

G

K

K

L

I

W

V

T

M

I

Y

D

D

L

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R

M

T

T

L

C

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Q

S

F

N

R

K

E

F

M

S

K

L

L

active site: R13 (= R5), D129 (= D121)
binding zinc ion: C171 (≠ S163)

Sites not aligning to the query:

binding zinc ion: 236, 239

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
36% identity, 100% coverage: 1:230/230 of query aligns to 9:215/217 of 1jwaB

query
sites
1jwaB

M

L

R

R

Y

Y

D

N

R
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R

Q

Q

M

I

L

I

L

L

P

R

E

G

I

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G

D

E

F

D

D

G

G

Q

Q

Q

E

K

A

L

L

K

K

Q

D

A

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K

R

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V

L

L

I

I

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V

G

G

V

L

G
|
G

L

L

G

G

S

C

P

A

I

A

A

S

L

Q

Y

Y

L

L

T

A

G

S

A

A

G

G

V

V

G

G

C

N

I

L

G

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D
|
D

D
x
F

D

D

V

T

V

V

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S

I

L

S

S

N

N

L

L

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Q

R
|
R

Q

Q

V

T

L

L

Y

H

S

S

E

D

K

A

E

T

L

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G

G

K

Q

P

P

K
|
K

A

V

I

E

C

S

A

A

A

R

E

D

R

A

L

L

S

T

A

R

L

I

N

N

S

P

E

H

I

I

T

A

I

I

R

T

T

P

Y

V

P

N

T

A

R
x
L

L

L

T

D

E

D

E

A

N

E

A

L

Q

A

E

A

I

L

I

I

S

A

Q

E

Y

H

D

D

I

L

V

V

V

L

D

D

G

C

C

T

D
|
D

N
|
N

F

V

S

A

T
x
V

R

R

Y

N

L

Q

I

L

N

N

D

A

I

G

C

C

A

F

E

A

M

A

G

K

K

V

V

P

Y

L

V

V

Y

S

G

G

A

A

I

A

C

I

G

R

F

M

E

E

G

G

Q

Q

V

I

S

T

V

V

F

F

H

T

Y

Y

G

-

E

-

K

-

S

-

Y

-

R

-

D

-

L

-

Y

-

P

-

D

-

E

-

M

-

R

-

M

-

P

-

P

E

K

A

G

G

V

V

M

M

G

A

I

P

T

L

P

I

A

G

V

V

T

I

G

G

S

S

I

L

E

Q

A

A

T

M

E

E

V

A

L

I

K

K

I

M

I

L

C

A

G

G

F

Y

G

G

E

K

V

P

L

A

S

S

G

G

K

K

L

I

W

V

T

M

I

Y

D

D

L

A

R

M

T

T

L

C

Q

Q

S

F

N

R

K

E

F

M

S

K

L

L

active site: R13 (= R5), D129 (= D121)
binding adenosine-5'-triphosphate: G39 (= G31), G40 (= G32), D61 (= D53), F62 (≠ D54), R72 (= R64), K85 (= K77), L108 (≠ R100), D129 (= D121), N130 (= N122), V133 (≠ T125)

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
41% identity, 95% coverage: 2:219/230 of query aligns to 12:228/270 of D4GSF3

query
sites
D4GSF3

R

R

Y

Y

D

S

R

R

Q

H

M

I

L

I

L

M

P

D

E

E

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A

S

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I

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A

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A

A

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Y

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A

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A

A

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G

G

C

E

I

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G

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L

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D

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E

I

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S

N

N

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L

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Q

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Q

Q

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D

E

D

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V

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G

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A

A

I

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A

A

E

A

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F

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R

A

G

L

L

N

N

S

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E

D

I

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S

I

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R

E

T

P

Y

V

P

E

T

A

R

R

L

V

T

D

E

K

E

S

N

N

A

V

Q

H

E

E

I

V

S

A

Q

G

Y

S

D

D

I

V

V

V

D

D

G

A

C

S

D

D

N

N

F

F

S

P

T

T

R

R

Y

Y

L

L

I

L

N

N

D

D

I

V

C

C

A

R

E

F

M

E

G

G

K

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P

Y

L

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V

Y

H

G

G

A

A

I

I

C

Y

G

K

F

F

E

E

G

G

Q

Q

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A

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T

F

L

H

V

Y

-

G

-

E

P

E

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K

G

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A

E

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E

M

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G

R

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M

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P

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P

D

-
x
C

P

A

P

T

K

T

G

G

V

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M

L

G

G

I

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T

L

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A

G

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C

I

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E

Q

A

A

T

T

E

E

V

A

L

M

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K

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C

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D

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E

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G

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A

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G

G

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L

W

L

T

F

I

Y

D

D

C188 (vs. gap) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
40% identity, 88% coverage: 2:204/230 of query aligns to 14:212/289 of 6yubB

query
sites
6yubB

R

R

Y

Y

D

S

R

R

Q

Q

M

M

L

I

L

V

P

P

E

G

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G

G

E

K

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E

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G

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K

M

Q

N

A

A

K

K

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L

I

I

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I

G

G

V

A

G

G

L

L

G

G

S

C

P

P

I

A

A

A

L

Q

Y

Y

L

L

T

A

G

G

A

A

G

G

V

V

G

G

C

T

I

I

G

G

L

I

V

V

D

D

D

G

D

D

V

S

V

V

S

E

I

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

L

A

Y

H

S

A

E

T

K

K

E

R

L

V

G

G

K

M

P

L

K

K

A

V

I

D

C

S

A

L

A

I

E

T

R

H

L

L

S

I

A

E

L

I

N

N

S

P

E

L

I

P

T

V

I

Y

R

V

T

P

Y

Y

P

R

T

F

R

D

L

L

T

T

E

P

E

Q

N

N

A

A

Q

A

E

Q

I

I

S

K

Q

P

Y

W

D

D

I

V

V

I

V

L

D

D

G

C

C

T

D

D

N

N

F

P

S

A

T

T

R

R

Y

Y

L

L

I

I

N

S

D

D

I

V

C

C

A

V

E

L

M

L

G

G

K

K

V

P

Y

L

V

V

Y

S

G

A

A

A

I

S

C

V

G

Q

F

K

E

S

G

G

Q

Q

V

L

S

I

V

V

F

L

H

N

Y

C

G

P

E

P

-

T

-

P

-

Q

-

G

-

V

E

N

K

K

S

A

-

A

-

P

-
x
C

Y

Y

R

R

D
x
C

L

C

Y

F

P

-

D

-

E

-

M

-

R

-

M

-

P

-

K

K

G

G

V

I

M

M

G

G

I

P

T

V

P

V

A

G

V

M

T

M

G

G

S

V

I

A

E

Q

A

A

T

G

E

E

V

A

L

I

K

K

I

I

I

L

binding zinc ion: C184 (vs. gap), C187 (≠ D166)

Sites not aligning to the query:

binding zinc ion: 265

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
40% identity, 88% coverage: 2:204/230 of query aligns to 13:213/423 of 6yubA

query
sites
6yubA

R

R

Y

Y

D

S

R

R

Q

Q

M

M

L

I

L

V

P

P

E

G

I

M

G

G

E

K

D

E

G

G

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Q

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K

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L

L

K

M

Q

N

A

A

K

K

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V

L

L

I

I

V

I

G

G

V

A

G

G

L

L

G

G

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C

P

P

I

A

A

A

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Y

Y

L

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T

A

G

G

A

A

G

G

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V

G

G

C

T

I

I

G

G

L

I

V

V

D

D

D

G

D

D

V

S

V

V

S

E

I

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

L

A

Y

H

S

A

E

T

K

K

E

R

L

V

G

G

K

M

P

L

K

K

A

V

I

D

C

S

A

L

A

I

E

T

R

H

L

L

S

I

A

E

L

I

N

N

S

P

E

L

I

P

T

V

I

Y

R

V

T

P

Y

Y

P

R

T

F

R

D

L

L

T

T

E

P

E

Q

N

N

A

A

Q

A

E

Q

I

I

S

K

Q

P

Y

W

D

D

I

V

V

I

V

L

D

D

G

C

C

T

D

D

N

N

F

P

S

A

T

T

R

R

Y

Y

L

L

I

I

N

S

D

D

I

V

C

C

A

V

E

L

M

L

G

G

K

K

V

P

Y

L

V

V

Y

S

G

A

A

A

I

S

C

V

G

Q

F

K

E

S

G

G

Q

Q

V

L

S

I

V

V

F

L

H

N

Y

C

G

P

E

P

E

T

K

P

K

Q

S

G

Y

V

-

V

-

N

R

K

D

K

L

A

Y

A

P

P

D
x
C

E

Y

E

R

E
x
C

M

C

R

F

R

K

M

K

P

P

P

-

K

-

G

G

V

I

M

M

G

G

I

P

T

V

P

V

A

G

V

M

T

M

G

G

S

V

I

A

E

Q

A

A

T

G

E

E

V

A

L

I

K

K

I

I

I

L

binding zinc ion: C183 (≠ D170), C186 (≠ E173)

Sites not aligning to the query:

binding zinc ion: 266, 269

O42939 Ubiquitin-activating enzyme E1-like; Pmt3-activating enzyme subunit 2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
33% identity, 62% coverage: 18:159/230 of query aligns to 21:163/628 of O42939

query
sites
O42939

Q

R

K

N

L

F

K

K

Q

S

A

A

K

K

V

V

L

L

I

L

V

V

G

G

V

A

G

G

L

I

G

G

S

C

P

E

I

L

A

L

L

K

Y

N

L

L

T

L

G

M

A

S

G

G

V

V

G

K

C

E

I

V

G

H

L

I

V

I

D

D

D

L

D

D

V

T

V

I

S

D

I

L

S

S

N

N

L

L

Q

N

R

R

Q

Q

V

F

L

L

Y

F

S

R

E

K

K

K

E

H

L

V

G

K

K

Q

P

P

K

K

A

A

I

I

C

V

A

A

E

K

R

T

L

A

S

S

A

S

L

F

N

N

S

P

E

N

I

V

T

K

I

L

R

E

T

A

Y

Y

P

H

T

A

R

N

L

I

T

K

E

E

E

D

N

R

A

F

Q

N

-

V

E

A

I

W

I

F

S

R

Q

Q

Y

F

D

D

I

L

V

V

V

F

D

N

G

A

C

L

D

D

N

N

F

L

S

D

T

A

R

R

Y

R

L

H

I

V

N

N

D

K

I

Q

C

C

A

L

E

L

M

A

G

S

K

V

V

P

Y

L

V

I

Y

E

G

S

A

G

I

T

C

T

G

G

F

F

E

L

G

G

Q

Q

V

V

S

Q

V

V

F

I

H

I

Y

H

G

G

E

K

Sites not aligning to the query:

559 modified: Phosphoserine
563 modified: Phosphoserine

3h5aC Crystal structure of e. Coli mccb (see paper)
28% identity, 91% coverage: 17:226/230 of query aligns to 110:336/358 of 3h5aC

query
sites
3h5aC

Q

Q

Q

D

K

K

L

L

K

K

Q

N

A

A

K

K

V

V

L

V

I

I

V

L

G

G

V

C

G

G

L

I

G

G

S

N

P

H

I

V

A

S

L

V

Y

I

L

L

T

A

G

T

A

S

G

G

V

I

G

G

C

E

I

I

G

I

L

L

V

I

D

D

D

N

D

D

V

Q

V

I

S

E

I

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

V

V

L

L

Y

F

S

S

E

E

K

D

E

D

L

V

G

G

K

K

P

N

K

K

A

T

I

E

C

V

A

I

A

K

E

R

R

E

L

L

S

L

A

K

L

R

N

N

S

S

E

E

I

I

T

S

I

V

R

S

T

E

Y

I

P

A

T

L

R

N

L

I

T

N

E

D

E

Y

N

T

A

D

Q

L

E

H

I

K

I

V

S

P

Q

E

Y

A

D

D

I

I

V

W

V

V

D

V

G

S

C

A

D

D

N

H

-

P

F

F

S

N

T

L

R

I

Y

N

L

W

I

V

N

N

D

K

I

Y

C

C

A

V

E

R

M

A

G

N

K

Q

V

P

Y

Y

V

I

Y

N

G

A

A

-

I

-

C

-

G

G

F

Y

E

V

G

N

Q

D

V

I

S

A

V

V

F

F

-

G

-

P

-

L

-

Y

-

V

-

P

-

G

H

K

Y

T

G

G

-
x
C

-

Y

E

E

E
x
C

K

Q

K

K

S

V

Y

V

R

A

D

D

L

L

Y

Y

P

G

D

S

E

E

E

K

E

E

-

N

-

I

-

D

-

H

-

K

-

I

-

K

-

L

-

I

M

N

R

S

R

R

M

F

P

K

P

P

P

-

K

-

G

A

V

T

M

F

G

A

I

P

T

V

P

N

A

N

V

V

T

A

G

A

S

A

I

L

E

C

A

A

T

A

E

D

V

V

L

I

K

K

I

F

I

I

C

G

G

K

F

Y

G

S

E

E

V

P

L

L

S

S

G

L

K

N

-

K

-

R

-

I

-

G

L

I

W

W

T

S

I

D

D

E

L

I

R

K

T

I

L

H

Q

S

S

Q

N

N

active site: R157 (= R64)
binding zinc ion: C257 (vs. gap), C260 (≠ E160)

Sites not aligning to the query:

binding zinc ion: 343

6om4B The structure of microcin c7 biosynthetic enzyme mccb in complex with n-formylated mcca (see paper)
28% identity, 91% coverage: 17:226/230 of query aligns to 107:333/344 of 6om4B

query
sites
6om4B

Q

Q

Q

D

K

K

L

L

K

K

Q

N

A

A

K

K

V

V

L

V

I

I

V

L

G

G

V

C

G
|
G

G

G

L
x
I

G

G

S

N

P

H

I

V

A

S

L

V

Y

I

L

L

T

A

G

T

A

S

G

G

V

I

G

G

C

E

I

I

G

I

L

L

V

I

D
|
D

D
x
N

D
|
D

V

Q

V

I

S
x
E

I

N

S

T

N
|
N

L

L

Q

T

R
|
R

Q
|
Q

V

V

L

L

Y

F

S

S

E

E

K

D

E

D

L

V

G

G

K

K

P

N

K
|
K

A

T

I

E

C

V

A

I

A

K

E

R

R

E

L

L

S

L

A

K

L

R

N

N

S

S

E

E

I

I

T

S

I

V

R

S

T

E

Y

I

P

A

T

L

R

N

L
x
I

T

N

E

D

E

Y

N

T

A

D

Q

L

E

H

I

K

I

V

S

P

Q

E

Y

A

D

D

I

I

V

W

V

V

D

V

G
x
S

C
x
A

D
|
D

N
x
H

-

P

F

F

S

N

T

L

R

I

Y

N

L

W

I

V

N

N

D

K

I

Y

C

C

A

V

E

R

M

A

G

N

K

Q

V

P

Y

Y

V

I

Y
x
N

G
x
A

A

-

I

-

C

-

G
|
G

F
x
Y

E
x
V

G

N

Q

D

V

I

S

A

V
|
V

F

F

-

G

-

P

-

L

-

Y

-

V

-

P

-

G

H

K

Y

T

G

G

-
x
C

-

Y

E

E

E
x
C

K

Q

K

K

S

V

Y
x
V

R
x
A

D

D

L

L

Y
|
Y

P

G

D

A

E

E

E

K

E

E

-

N

-

I

-

D

-

H

-

K

-

I

-

K

-

L

-

I

M

N

R

S

R

R

M

F

P

K

P

P

P

-

K

-

G

A

V

T

M

F

G

A

I

P

T

V

P

N

A

N

V

V

T

A

G

A

S

A

I

L

E

C

A

A

T

A

E

D

V

V

L

I

K

K

I

F

I

I

C

G

G

K

F

Y

G

S

E

E

V

P

L

L

S

S

G

L

K

N

-

K

-
x
R

-

I

-
x
G

L

I

W
|
W

T

S

I

D

D

E

L

I

R

K

T

I

L

H

Q

S

S
x
Q

N

N

binding magnesium ion: D145 (= D55), E148 (≠ S58)
binding 5'-O-[(S)-amino(hydroxy)phosphoryl]adenosine: G121 (= G31), D143 (= D53), N144 (≠ D54), K167 (= K77), I191 (≠ L101), S209 (≠ G119), A210 (≠ C120), D211 (= D121), H212 (≠ N122)
binding pyrophosphate 2-: N151 (= N61), R154 (= R64), Q155 (= Q65), K167 (= K77)
binding zinc ion: C254 (vs. gap), C257 (≠ E160)
binding : I123 (≠ L33), S209 (≠ G119), A210 (≠ C120), D211 (= D121), N233 (≠ Y142), A234 (≠ G143), G235 (= G147), Y236 (≠ F148), V237 (≠ E149), V242 (= V154), V261 (≠ Y164), A262 (≠ R165), Y265 (= Y168), R319 (vs. gap), G321 (vs. gap), W323 (= W216), Q332 (≠ S225)

Sites not aligning to the query:

binding zinc ion: 340, 343
binding : 10, 14, 23, 26

6om4A The structure of microcin c7 biosynthetic enzyme mccb in complex with n-formylated mcca (see paper)
28% identity, 91% coverage: 17:226/230 of query aligns to 107:333/345 of 6om4A

query
sites
6om4A

Q

Q

Q

D

K

K

L

L

K

K

Q

N

A

A

K

K

V

V

L

V

I

I

V

L

G

G

V

C

G
|
G

G

G

L
x
I

G

G

S

N

P

H

I

V

A

S

L

V

Y

I

L

L

T

A

G

T

A

S

G

G

V

I

G

G

C

E

I

I

G

I

L

L

V

I

D
|
D

D

N

D

D

V

Q

V

I

S

E

I

N

S

T

N
|
N

L

L

Q

T

R
|
R

Q

Q

V

V

L

L

Y

F

S

S

E

E

K

D

E

D

L

V

G

G

K

K

P

N

K
|
K

A

T

I

E

C

V

A

I

A

K

E

R

R

E

L

L

S

L

A

K

L

R

N

N

S

S

E

E

I

I

T

S

I

V

R

S

T

E

Y

I

P

A

T

L

R
x
N

L
x
I

T

N

E

D

E

Y

N

T

A

D

Q

L

E

H

I

K

I

V

S

P

Q

E

Y

A

D

D

I

I

V

W

V

V

D

V

G
x
S

C
x
A

D
|
D

N
x
H

-

P

F

F

S

N

T

L

R

I

Y

N

L

W

I

V

N

N

D

K

I

Y

C

C

A

V

E

R

M

A

G

N

K

Q

V

P

Y

Y

V

I

Y
x
N

G
x
A

A

-

I

-

C

-

G
|
G

F
x
Y

E
x
V

G

N

Q

D

V

I

S

A

V

V

F

F

-

G

-

P

-

L

-

Y

-

V

-

P

-

G

H

K

Y

T

G

G

-
x
C

-

Y

E

E

E
x
C

K

Q

K

K

S

V

Y
x
V

R
x
A

D

D

L

L

Y
|
Y

P

G

D

A

E

E

E

K

E

E

-

N

-

I

-

D

-

H

-

K

-

I

-

K

-

L

-

I

M

N

R

S

R

R

M

F

P

K

P

P

P

-

K

-

G

A

V

T

M

F

G

A

I

P

T

V

P

N

A

N

V

V

T

A

G

A

S

A

I

L

E

C

A

A

T

A

E

D

V

V

L

I

K

K

I

F

I

I

C

G

G

K

F

Y

G

S

E

E

V

P

L

L

S

S

G

L

K

N

-

K

-
x
R

-

I

-
x
G

L

I

W
|
W

T

S

I

D

D

E

L

I

R

K

T

I

L

H

Q

S

S
x
Q

N

N

binding 5'-O-[(S)-amino(hydroxy)phosphoryl]adenosine: G121 (= G31), D143 (= D53), K167 (= K77), N190 (≠ R100), I191 (≠ L101), S209 (≠ G119), A210 (≠ C120), D211 (= D121), H212 (≠ N122)
binding pyrophosphate 2-: N151 (= N61), R154 (= R64), K167 (= K77)
binding zinc ion: C254 (vs. gap), C257 (≠ E160)
binding : I123 (≠ L33), S209 (≠ G119), A210 (≠ C120), D211 (= D121), N233 (≠ Y142), A234 (≠ G143), G235 (= G147), Y236 (≠ F148), V237 (≠ E149), V261 (≠ Y164), A262 (≠ R165), Y265 (= Y168), R319 (vs. gap), G321 (vs. gap), W323 (= W216), Q332 (≠ S225)

Sites not aligning to the query:

binding zinc ion: 340, 343
binding : 10, 19, 23, 26

3h9jA Crystal structure of e. Coli mccb + ampcpp + semet mcca (see paper)
28% identity, 91% coverage: 17:226/230 of query aligns to 110:327/339 of 3h9jA

query
sites
3h9jA

Q

Q

Q

D

K

K

L

L

K

K

Q

N

A

A

K

K

V

V

L

V

I

I

V

L

G

G

V

C

G
|
G

L

I

G

G

S

N

P

H

I

V

A

S

L

V

Y

I

L

L

T

A

G

T

A

S

G

G

V

I

G

G

C

E

I

I

G

I

L

L

V

I

D
|
D

D

N

D
|
D

V

Q

V

I

S

E

I

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

V

V

L

L

Y

F

S

S

E

E

K

D

E

D

L

V

G

G

K

K

P

N

K
|
K

A

T

I

E

C

V

A

I

A

K

E

R

R

E

L

L

S

L

A

K

L

R

N

N

S

S

E

E

I

I

T

S

I

V

R

S

T

E

Y

I

P

A

T

L

R
x
N

L
x
I

T

N

E

D

E

Y

N

T

A

D

Q

L

E

H

I

K

I

V

S

P

Q

E

Y

A

D

D

I

I

V

W

V

V

D

V

G

S

C
x
A

D
|
D

N
x
H

-

P

F

F

S

N

T

L

R

I

Y

N

L

W

I

V

N

N

D

K

I

Y

C

C

A

V

E

R

M

A

G

N

K

Q

V

P

Y

Y

V

I

Y

N

G

A

A

-

I

-

C

-

G

G

F

Y

E

V

G

N

Q

D

V

I

S

A

V

V

F

F

H

G

-

P

-

L

-

Y

-

V

-

P

-

G

-

K

-

T

-

G

-
x
C

Y

Y

G

E

E

C

E

Q

K

K

S

E

Y

N

R

I

D

D

L

H

Y

K

P

I

D

K

E

L

E

I

E

N

M

S

R

R

R

F

M

K

P

P

P

A

P

T

P

F

K

A

G

P

V

V

M

N

G

N

I

V

T

A

P

A

A

A

V

L

T

C

G

-

S

-

I

-

E

-

A

A

T

A

E

D

V

V

L

I

K

K

I

F

I

I

C

G

G

K

F

Y

G

S

E

E

V

P

L

L

S

S

G

L

K

N

-

K

-
x
R

-

I

-
x
G

L

I

W

W

T

S

I

D

D

E

L

I

R

K

T

I

L

H

Q

S

S

Q

N

N

active site: R157 (= R64)
binding diphosphomethylphosphonic acid adenosyl ester: G124 (= G31), G125 (= G32), D146 (= D53), D148 (= D55), R157 (= R64), K170 (= K77), N193 (≠ R100), I194 (≠ L101), A213 (≠ C120), D214 (= D121), H215 (≠ N122)
binding zinc ion: C257 (vs. gap)
binding : R313 (vs. gap), G315 (vs. gap)

Sites not aligning to the query:

binding zinc ion: 334, 337

Query Sequence

>350176 FitnessBrowser__Btheta:350176
MRYDRQMLLPEIGEDGQQKLKQAKVLIVGVGGLGSPIALYLTGAGVGCIGLVDDDVVSIS
NLQRQVLYSEKELGKPKAICAAERLSALNSEITIRTYPTRLTEENAQEIISQYDIVVDGC
DNFSTRYLINDICAEMGKVYVYGAICGFEGQVSVFHYGEEKKSYRDLYPDEEEMRRMPPP
PKGVMGITPAVTGSIEATEVLKIICGFGEVLSGKLWTIDLRTLQSNKFSL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory