SitesBLAST

D10 (= D14) mutation to N: Loss of catalytic activity.
E17 (= E21) mutation to A: 40% to 80% decreased catalytic activity with xylulose-1,5-bisphosphate, but no effect on activity with ribulose-1,5-bisphosphate.
H20 (≠ Y24) mutation to A: 40% to 80% decreased catalytic activity with xylulose-1,5-bisphosphate, but no effect on activity with ribulose-1,5-bisphosphate.
Y42 (≠ G32) mutation to A: 40% to 80% decreased catalytic activity with xylulose-1,5-bisphosphate, but no effect on activity with ribulose-1,5-bisphosphate.
R54 (≠ D40) mutation to A: 97% decreased catalytic activity with xylulose-1,5-bisphosphate, but no effect on activity with ribulose-1,5-bisphosphate.
K78 (≠ Q65) mutation to A: 40% to 80% decreased catalytic activity with xylulose-1,5-bisphosphate, but no effect on activity with ribulose-1,5-bisphosphate.

4uauA Crystal structure of cbby (mutant d10n) from rhodobacter sphaeroides in complex with xylulose-(1,5)bisphosphate, crystal form ii (see paper)
27% identity, 92% coverage: 8:205/215 of query aligns to 4:217/226 of 4uauA

query
sites
4uauA

A

A

A

I

L

L

F

F

D
|
D

F
x
V

D
x
N

G

G

V

T

I

L

M

A

D

E

T
|
T

E
|
E

T

E

Q

L

Y
x
H

T

R

V

R

-

A

-

F

-

N

-

E

-

T

-

F

-

A

-

L

-

G

-

V

-

D

-

W

F

F

W

W

D

D

-

R

E

E

Q

E

G

Y

R

R

K

E

Y

L

L

L

-
x
T

-

T

-
x
T

-
x
G

N
x
G

E

K

E

E

D
x
R

F

I

G

A

R

R

R

F

I

L

K

R

G

H

Q

Q

T

K

L

-

T

G

Q

D

I

P

Y

A

E

P

K

L

Y

P

F

I

S

A

D

D

L

I

P
x
H

E

R

A

A

Q
x
K

Q

T

E

E

I

R

T

F

A

V

G

A

L

L

N

-

I

M

Y

A

E

E

K

G

S

E

M

I

S

A

Y

L

E

R

Y

-

I

-

P

P

G

G

V

I

E

A

A

D

F

L

I

I

A

A

D

E

L

A

R

K

K

R

H

A

G

G

A

I

K

R

I

L

A

A

V

V

V

A

T
|
T

S
x
T

-
x
S

-

L

-

P

N

N

E

V

E

E

K

A

M

L

Q

C

N

R

V

A

Y

C

N

F

A

G

H

H

P

P

E

A

F

-

K

R

G

E

M

I

V

F

D

D

R

V

I

I

L

A

T

A

G

G

E

D

M

M

F

V

A

A

R

E

S

K

K
|
K

P

P

A

S

P

P

D

D

C

I

F

Y

L

R

L

L

G

A

M

L

E

R

I

E

F

L

G

D

A

V

T

P

P

P

E

E

N

R

S

A

Y

V

V

A

F

L

E
|
E

D
|
D

S

S

F

L

H

N

G

G

L

L

Q

R

A

A

G

A

M

K

T

G

S

A

G

G

A

L

T

R

V

C

I

I

G

V

L

S

A

P

T

G

T

F

N

Y

T

T

R

R

E

H

A

E

I

E

T

F

G

A

K

G

A

A

H

D

Y

R

I

L

D

D

D

S

F

F

S

A

E

E

M

L

active site: D8 (= D12), V9 (≠ F13), N10 (≠ D14), T16 (= T20), T47 (vs. gap), T115 (= T105), T116 (≠ S106), K151 (= K139), E175 (= E163), D176 (= D164)
binding magnesium ion: D8 (= D12), N10 (≠ D14), D176 (= D164)
binding xylulose-1,5-bisphosphate: D8 (= D12), V9 (≠ F13), N10 (≠ D14), E17 (= E21), H20 (≠ Y24), T49 (vs. gap), G50 (vs. gap), G51 (≠ N37), R54 (≠ D40), H75 (≠ P62), K78 (≠ Q65), T115 (= T105), T116 (≠ S106), T117 (≠ S107), S118 (vs. gap)

Q8VZ10 Protein SUPPRESSOR OF QUENCHING 1, chloroplastic; EC 3.1.3.- from Arabidopsis thaliana (Mouse-ear cress) (see paper)
27% identity, 98% coverage: 2:211/215 of query aligns to 70:290/1055 of Q8VZ10

query
sites
Q8VZ10

D

D

T

W

T

G

K

K

T

V

I

S

A

A

A

V

L

L

F

F

D
|
D

F

M

D

D

G

G

V

V

I

L

M

C

D

N

T

S

E

E

T

D

-

L

-

S

-

R

-

A

-

V

-

D

-

V

-

F

-

T

-

E

-

M

-

G

-

V

-

E

-

V

-

T

-

V

-

D

Q

D

Y

F

T

V

V

P

F

F

W

M

D

G

E

T

Q

G

G

E

R

A

K

K

Y

F

L

L

N

G

E

G

E

V

D

A

F

S

G

V

R

K

R

E

I

V

K

K

G

G

Q

F

T

D

L

P

T

D

Q

A

I

A

Y

K

E

E

K

R

Y

F

F

F

S

-

D

-

L

-

P

-

E

-

A

-

Q

-

E

E

I

I

T

Y

A

-

G

-

L

L

N

D

I

K

Y

Y

E

A

K

K

S

P

M

E

S

S

Y

G

E

I

Y

G

I

F

P

P

G

G

V

A

E

L

A

E

F

L

I

V

A

T

D

E

L

C

R

K

K

N

H

K

G

G

A

L

K

K

I

V

A

A

V

V

V

A

T

S

S

S

S

A

N

D

E

R

E

I

K

K

M

V

Q

D

N

A

V

N

Y

L

N

K

A

A

H

A

P

G

E

L

F

S

K

L

G

T

M

M

V

F

D

D

R

A

I

I

L

V

T

S

G

A

E

D

M

A

F

F

A

E

R

N

S

L

K

K

P

P

A

A

P

P

D

D

C

I

F

F

L

L

L

A

G

A

M

A

E

K

I

I

F

L

G

G

A

V

T

P

P

T

E

S

N

E

S

C

Y

V

V

V

F

I

E

E

D

D

S

A

F

L

H

A

G

G

L

V

Q

Q

A

A

G

A

M

Q

T

A

S

A

G

N

A

M

T

R

V

C

I

I

G

A

L

V

A

K

T

T

N

-

T

L

R

S

E

E

A

A

I

I

T

L

G

K

K

D

A

A

-

G

-

P

H

S

Y

M

I

I

I

R

D

D

F

I

S

G

E

N

M

I

T

S

F

I

E

N

H

D

L

I

L

L

D80 (= D12) mutation to N: Complete rescue in complementation test of the nonphotochemical quenching (NPQ) phenotype observed in disrupted plants.

Sites not aligning to the query:

431:434 CINC→SINS: No rescue in complementation test of the nonphotochemical quenching (NPQ) phenotype observed in disrupted plants.
859 E→K: In soq1-2; high light intensity-dependent and irreversible nonphotochemical quenching (NPQ) due to a decrease in chlorophyll excited-state lifetime.

4c4sA Structure of beta-phosphoglucomutase in complex with an alpha- fluorophosphonate analogue of beta-glucose-1-phosphate and magnesium trifluoride (see paper)
30% identity, 94% coverage: 8:210/215 of query aligns to 4:209/215 of 4c4sA

query
sites
4c4sA

A

A

A

V

L

L

F

F

D
|
D

F
x
L

D
|
D

G

G

V

V

I

I

M

T

D

D

T
|
T

E

A

T

E

Q

Y

Y
x
H

T

F

V

R

F

A

W
|
W

D

-

E

-

Q

-

G

-

R

-

K

K

Y

A

L

L

N

A

E

E

-

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

D

Q

F

F

G

N

R

E

R

Q

I
x
L

K
|
K

G
|
G

-
x
V

-

S

-
x
R

-

E

Q

D

T

S

L

L

T

Q

Q

K

I

I

Y

L

E

D

-

L

-

K

-

V

-

S

-

A

K

E

Y

E

F

F

S

K

D

E

L

L

P

-

E

-

A

A

Q

K

Q

R

E

K

I

N

T

D

A

N

G

Y

L

V

N

K

I

M

Y

I

E

Q

K

D

S

V

M

S

S

P

Y

A

E

D

Y

V

I

Y

P

P

G

G

V

I

E

L

A

Q

F

L

I

L

A

K

D

D

L

L

R

R

K

S

H

N

G

K

A

I

K

K

I

I

A

A

V

L

V

A

T
x
S

S
x
A

S
|
S

N

K

E

N

-

G

-

P

-

F

-

L

E

E

K

R

M

M

Q

N

N

-

V

-

Y

-

N

-

A

-

H

-

P

-

E

-

F

L

K

T

G

G

M

Y

V

F

D

D

R

A

I

I

L

A

T

D

G

P

E

A

M

E

F

V

A

A

R

A

S

S

K
|
K

P

P

A

A

P

P

D

D

C

I

F

F

L

I

L

A

G

A

M

A

E

H

I

A

F

V

G

G

A

V

T

A

P

P

E

S

N

E

S

S

Y

I

V

G

F

L

E
|
E

D
|
D

S

S

F

Q

H

A

G

G

L

I

Q

Q

A

A

G

I

M

K

T

D

S

S

G

G

A

A

T

L

V

P

I

I

G

G

L

V

A

G

T

-

N

-

T

-

R

R

E

P

A

E

I

D

T

L

G

G

K

D

A

D

H

I

Y

V

I

I

I

V

D

P

D

D

F

T

S

S

E

H

M

Y

T

T

F

L

E

E

H

F

L

L

active site: D8 (= D12), L9 (≠ F13), D10 (= D14), T16 (= T20), K45 (= K46), S111 (≠ T105), A112 (≠ S106), K142 (= K139), E166 (= E163), D167 (= D164)
binding (1R)-1,5-anhydro-1-[(S)-fluoro(phosphono)methyl]-D-glucitol: D10 (= D14), H20 (≠ Y24), W24 (= W28), L44 (≠ I45), G46 (= G47), V47 (vs. gap), R49 (vs. gap), S113 (= S107)
binding magnesium ion: D8 (= D12), D10 (= D14), D167 (= D164)
binding trifluoromagnesate: D8 (= D12), L9 (≠ F13), D10 (= D14), S111 (≠ T105), A112 (≠ S106), K142 (= K139)

5olwA 5-fluorotryptophan labeled beta-phosphoglucomutase in an open conformation (see paper)
30% identity, 94% coverage: 8:210/215 of query aligns to 4:212/224 of 5olwA

query
sites
5olwA

A

A

A

V

L

L

F

F

D
|
D

F
x
L

D
|
D

G

G

V

V

I

I

M

T

D

D

T
|
T

E

A

T

E

Q

Y

Y

H

T

F

V

R

F

A

W

W

D

-

E

-

Q

-

G

-

R

-

K

K

Y

A

L

L

N

A

E

E

-

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

D

Q

F

F

G

N

R

E

R

Q

I

L

K
|
K

G

G

-

V

-

S

-

R

-

E

-

D

-

S

-

L

Q

Q

T

K

L

I

T

L

Q

D

I

L

Y

A

E

D

K

K

Y

K

F

V

S

S

D

-

L

-

P

A

E

E

A

E

Q

F

Q

K

E

E

I

L

T

A

A

K

G

R

L

K

N

N

-

D

-

N

-

Y

-

V

-

K

I

M

Y

I

E

Q

K

D

S

V

M

S

S
x
P

Y

A

E

D

Y
x
V

I

Y

P

P

G

G

V

I

E

L

A

Q

F

L

I

L

A

K

D

D

L

L

R

R

K

S

H

N

G

K

A

I

K

K

I

I

A

A

V

L

V

A

T
x
S

S
x
A

S

S

N

K

E

N

-

G

-

P

-

F

-

L

E
|
E

K

R

M

M

Q
x
N

N

-

V

-

Y

-

N

-

A

-

H

-

P

-

E

-

F

L

K

T

G

G

M

Y

V

F

D

D

R

A

I

I

L

A

T

D

G

P

E

A

M

E

F

V

A

A

R

A

S

S

K
|
K

P

P

A

A

P

P

D

D

C

I

F

F

L

I

L

A

G

A

M

A

E

H

I

A

F

V

G

G

A

V

T

A

P

P

E

S

N

E

S

S

Y

I

V

G

F

L

E
|
E

D
|
D

S
|
S

F

Q

H

A

G

G

L

I

Q

Q

A

A

G

I

M

K

T

D

S

S

G

G

A

A

T

L

V

P

I

I

G

G

L

V

A

G

T

-

N

-

T

-

R

R

E

P

A

E

I

D

T

L

G

G

K

D

A

D

H

I

Y

V

I

I

I

V

D

P

D

D

F

T

S

S

E

H

M

Y

T

T

F

L

E

E

H

F

L

L

active site: D8 (= D12), L9 (≠ F13), D10 (= D14), T16 (= T20), K45 (= K46), S114 (≠ T105), A115 (≠ S106), K145 (= K139), E169 (= E163), D170 (= D164)
binding calcium ion: D8 (= D12), D10 (= D14), P89 (≠ S80), V92 (≠ Y83), E124 (= E110), N127 (≠ Q113), E169 (= E163), D170 (= D164), S171 (= S165)

6h91A Phosphorylated beta-phosphoglucomutase from lactococcus lactis in an open conformer to 2.4 a
30% identity, 94% coverage: 8:210/215 of query aligns to 4:212/218 of 6h91A

query
sites
6h91A

A

A

A

V

L

L

F

F

D
|
D

F

L

D
|
D

G

G

V

V

I

I

M

T

D

D

T

T

E

A

T

E

Q

Y

Y

H

T

F

V

R

F

A

W

W

D

-

E

-

Q

-

G

-

R

-

K

K

Y

A

L

L

N

A

E

E

-

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

D

Q

F

F

G

N

R

E

R

Q

I

L

K

K

G

G

-

V

-

S

-
x
R

-

E

-

D

-

S

-

L

Q

Q

T

K

L

I

T

L

Q

D

I

L

Y

A

E

D

K

K

Y

K

F

V

S

S

D

-

L

-

P

A

E

E

A

E

Q

F

Q

K

E

E

I

L

T

A

A

K

G

R

L

K

N

N

-

D

-

N

-

Y

-

V

-

K

I

M

Y

I

E

Q

K

D

S

V

M

S

S

P

Y

A

E

D

Y

V

I

Y

P

P

G

G

V

I

E

L

A

Q

F

L

I

L

A

K

D

D

L

L

R

R

K

S

H

N

G

K

A

I

K

K

I

I

A

A

V

L

V

A

T

S

S

A

S

S

N
x
K

E

N

-

G

-

P

-

F

-

L

E

E

K

R

M

M

Q

N

N

-

V

-

Y

-

N

-

A

-

H

-

P

-

E

-

F

L

K

T

G

G

M

Y

V

F

D

D

R

A

I

I

L

A

T

D

G

P

E

A

M

E

F

V

A

A

R

A

S

S

K

K

P

P

A

A

P

P

D

D

C

I

F

F

L

I

L

A

G

A

M

A

E

H

I

A

F

V

G

G

A

V

T

A

P

P

E

S

N

E

S

S

Y

I

V

G

F

L

E

E

D
|
D

S

S

F

Q

H

A

G

G

L

I

Q

Q

A

A

G

I

M

K

T

D

S

S

G

G

A

A

T

L

V

P

I

I

G

G

L

V

A

G

T

-

N

-

T

-

R

R

E

P

A

E

I

D

T

L

G

G

K

D

A

D

H

I

Y

V

I

I

I

V

D

P

D

D

F

T

S

S

E

H

M

Y

T

T

F

L

E

E

H

F

L

L

binding magnesium ion: D8 (= D12), D10 (= D14), D170 (= D164)
binding acetylphosphate: R49 (vs. gap), K117 (≠ N108)

4c4rA Structure of beta-phosphoglucomutase in complex with a phosphonate analogue of beta-glucose-1-phosphate and magnesium trifluoride (see paper)
30% identity, 94% coverage: 8:210/215 of query aligns to 4:212/218 of 4c4rA

query
sites
4c4rA

A

A

A

V

L

L

F

F

D
|
D

F
x
L

D
|
D

G

G

V

V

I

I

M

T

D

D

T
|
T

E

A

T

E

Q

Y

Y
x
H

T

F

V

R

F

A

W
|
W

D

-

E

-

Q

-

G

-

R

-

K

K

Y

A

L

L

N

A

E

E

-

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

D

Q

F

F

G

N

R

E

R

Q

I
x
L

K
|
K

G
|
G

-
x
V

-

S

-
x
R

-

E

-

D

-
x
S

-

L

Q

Q

T

K

L

I

T

L

Q

D

I

L

Y

A

E

D

K

K

Y

K

F

V

S

S

D

-

L

-

P

A

E

E

A

E

Q

F

Q

K

E

E

I

L

T

A

A

K

G

R

L

K

N

N

-

D

-

N

-

Y

-

V

-

K

I

M

Y

I

E

Q

K

D

S

V

M

S

S

P

Y

A

E

D

Y

V

I

Y

P

P

G

G

V

I

E

L

A

Q

F

L

I

L

A

K

D

D

L

L

R

R

K

S

H

N

G

K

A

I

K

K

I

I

A

A

V

L

V

A

T
x
S

S
x
A

S
|
S

N
x
K

E

N

-

G

-

P

-

F

-

L

E

E

K

R

M

M

Q

N

N

-

V

-

Y

-

N

-

A

-

H

-

P

-

E

-

F

L

K

T

G

G

M

Y

V

F

D

D

R

A

I

I

L

A

T

D

G

P

E

A

M

E

F

V

A

A

R

A

S

S

K
|
K

P

P

A

A

P

P

D

D

C

I

F

F

L

I

L

A

G

A

M

A

E

H

I

A

F

V

G

G

A

V

T

A

P

P

E

S

N

E

S

S

Y

I

V

G

F

L

E
|
E

D
|
D

S

S

F

Q

H

A

G

G

L

I

Q

Q

A

A

G

I

M

K

T

D

S

S

G

G

A

A

T

L

V

P

I

I

G

G

L

V

A

G

T

-

N

-

T

-

R

R

E

P

A

E

I

D

T

L

G

G

K

D

A

D

H

I

Y

V

I

I

I

V

D

P

D

D

F

T

S

S

E

H

M

Y

T

T

F

L

E

E

H

F

L

L

active site: D8 (= D12), L9 (≠ F13), D10 (= D14), T16 (= T20), K45 (= K46), S114 (≠ T105), A115 (≠ S106), K145 (= K139), E169 (= E163), D170 (= D164)
binding magnesium ion: D8 (= D12), D10 (= D14), D170 (= D164)
binding trifluoromagnesate: D8 (= D12), L9 (≠ F13), D10 (= D14), S114 (≠ T105), A115 (≠ S106), K145 (= K139)
binding (1R)-1,5-anhydro-1-(phosphonomethyl)-D-glucitol: D10 (= D14), H20 (≠ Y24), W24 (= W28), L44 (≠ I45), G46 (= G47), V47 (vs. gap), R49 (vs. gap), S52 (vs. gap), S116 (= S107), K117 (≠ N108)

3zi4A The structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate and scandium tetrafluoride
30% identity, 94% coverage: 8:210/215 of query aligns to 4:212/218 of 3zi4A

query
sites
3zi4A

A

A

A

V

L

L

F

F

D
|
D

F
x
L

D
|
D

G

G

V

V

I

I

M

T

D

D

T
|
T

E

A

T

E

Q

Y

Y
x
H

T

F

V

R

F

A

W

W

D

-

E

-

Q

-

G

-

R

-

K

K

Y

A

L

L

N

A

E

E

-

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

D

Q

F

F

G

N

R

E

R

Q

I

L

K
|
K

G
|
G

-
x
V

-

S

-
x
R

-

E

-

D

-

S

-

L

Q

Q

T

K

L

I

T

L

Q

D

I

L

Y

A

E

D

K

K

Y

K

F

V

S

S

D

-

L

-

P

A

E

E

A

E

Q

F

Q

K

E

E

I

L

T

A

A

K

G

R

L

K

N

N

-

D

-

N

-

Y

-

V

-

K

I

M

Y

I

E

Q

K

D

S

V

M

S

S

P

Y

A

E

D

Y

V

I

Y

P

P

G

G

V

I

E

L

A

Q

F

L

I

L

A

K

D

D

L

L

R

R

K

S

H

N

G

K

A

I

K

K

I

I

A

A

V

L

V

A

T
x
S

S
x
A

S
|
S

N
x
K

E

N

-

G

-

P

-

F

-

L

E

E

K

R

M

M

Q

N

N

-

V

-

Y

-

N

-

A

-

H

-

P

-

E

-

F

L

K

T

G

G

M

Y

V

F

D

D

R

A

I

I

L

A

T

D

G

P

E

A

M

E

F

V

A

A

R

A

S

S

K
|
K

P

P

A

A

P

P

D

D

C

I

F

F

L

I

L

A

G

A

M

A

E

H

I

A

F

V

G

G

A

V

T

A

P

P

E

S

N

E

S

S

Y

I

V

G

F

L

E
|
E

D
|
D

S

S

F

Q

H

A

G

G

L

I

Q

Q

A

A

G

I

M

K

T

D

S

S

G

G

A

A

T

L

V

P

I

I

G

G

L

V

A

G

T

-

N

-

T

-

R

R

E

P

A

E

I

D

T

L

G

G

K

D

A

D

H

I

Y

V

I

I

I

V

D

P

D

D

F

T

S

S

E

H

M

Y

T

T

F

L

E

E

H

F

L

L

active site: D8 (= D12), L9 (≠ F13), D10 (= D14), T16 (= T20), K45 (= K46), S114 (≠ T105), A115 (≠ S106), K145 (= K139), E169 (= E163), D170 (= D164)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D14), H20 (≠ Y24), G46 (= G47), V47 (vs. gap), R49 (vs. gap), S116 (= S107), K117 (≠ N108)
binding magnesium ion: D8 (= D12), D10 (= D14), D170 (= D164)
binding Scandium Tetrafluoride: D8 (= D12), L9 (≠ F13), D10 (= D14), S114 (≠ T105), A115 (≠ S106), K145 (= K139)

2wf8A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate, glucose-1-phosphate and beryllium trifluoride (see paper)
30% identity, 94% coverage: 8:210/215 of query aligns to 4:212/218 of 2wf8A

query
sites
2wf8A

A

A

A

V

L

L

F

F

D
|
D

F
x
L

D
|
D

G

G

V

V

I

I

M

T

D

D

T
|
T

E

A

T

E

Q

Y

Y
x
H

T

F

V

R

F

A

W
|
W

D

-

E

-

Q

-

G

-

R

-

K

K

Y

A

L

L

N

A

E

E

-

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

D

Q

F

F

G

N

R

E

R

Q

I
x
L

K
|
K

G
|
G

-
x
V

-

S

-
x
R

-

E

-

D

-
x
S

-

L

Q

Q

T

K

L

I

T

L

Q

D

I

L

Y

A

E

D

K

K

Y

K

F

V

S

S

D

-

L

-

P

A

E

E

A

E

Q

F

Q

K

E

E

I

L

T

A

A

K

G

R

L

K

N

N

-

D

-

N

-

Y

-

V

-

K

I

M

Y

I

E

Q

K

D

S

V

M

S

S

P

Y

A

E

D

Y

V

I

Y

P

P

G

G

V

I

E

L

A

Q

F

L

I

L

A

K

D

D

L

L

R

R

K

S

H

N

G

K

A

I

K

K

I

I

A

A

V

L

V

A

T
x
S

S
x
A

S
|
S

N
x
K

E

N

-

G

-

P

-

F

-

L

E

E

K

R

M

M

Q

N

N

-

V

-

Y

-

N

-

A

-

H

-

P

-

E

-

F

L

K

T

G

G

M

Y

V

F

D

D

R

A

I

I

L

A

T

D

G

P

E

A

M

E

F

V

A

A

R

A

S

S

K
|
K

P

P

A

A

P

P

D

D

C

I

F

F

L

I

L

A

G

A

M

A

E

H

I

A

F

V

G

G

A

V

T

A

P

P

E

S

N

E

S

S

Y

I

V

G

F

L

E
|
E

D
|
D

S

S

F

Q

H

A

G

G

L

I

Q

Q

A

A

G

I

M

K

T

D

S

S

G

G

A

A

T

L

V

P

I

I

G

G

L

V

A

G

T

-

N

-

T

-

R

R

E

P

A

E

I

D

T

L

G

G

K

D

A

D

H

I

Y

V

I

I

I

V

D

P

D

D

F

T

S

S

E

H

M

Y

T

T

F

L

E

E

H

F

L

L

active site: D8 (= D12), L9 (≠ F13), D10 (= D14), T16 (= T20), K45 (= K46), S114 (≠ T105), A115 (≠ S106), K145 (= K139), E169 (= E163), D170 (= D164)
binding beryllium trifluoride ion: D8 (= D12), L9 (≠ F13), D10 (= D14), S114 (≠ T105), A115 (≠ S106), K145 (= K139)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D14), H20 (≠ Y24), G46 (= G47), V47 (vs. gap), R49 (vs. gap), A115 (≠ S106), S116 (= S107), K117 (≠ N108)
binding 1-O-phosphono-alpha-D-glucopyranose: D10 (= D14), H20 (≠ Y24), W24 (= W28), L44 (≠ I45), G46 (= G47), V47 (vs. gap), R49 (vs. gap), S52 (vs. gap), A115 (≠ S106), S116 (= S107), K117 (≠ N108)
binding magnesium ion: D8 (= D12), D10 (= D14), D170 (= D164)

2wf7A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphonate and aluminium tetrafluoride (see paper)
30% identity, 94% coverage: 8:210/215 of query aligns to 4:212/218 of 2wf7A

query
sites
2wf7A

A

A

A

V

L

L

F

F

D
|
D

F
x
L

D
|
D

G

G

V

V

I

I

M

T

D

D

T
|
T

E

A

T

E

Q

Y

Y

H

T

F

V

R

F

A

W

W

D

-

E

-

Q

-

G

-

R

-

K

K

Y

A

L

L

N

A

E

E

-

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

D

Q

F

F

G

N

R

E

R

Q

I

L

K
|
K

G
|
G

-
x
V

-

S

-
x
R

-

E

-

D

-

S

-

L

Q

Q

T

K

L

I

T

L

Q

D

I

L

Y

A

E

D

K

K

Y

K

F

V

S

S

D

-

L

-

P

A

E

E

A

E

Q

F

Q

K

E

E

I

L

T

A

A

K

G

R

L

K

N

N

-

D

-

N

-

Y

-

V

-

K

I

M

Y

I

E

Q

K

D

S

V

M

S

S

P

Y

A

E

D

Y

V

I

Y

P

P

G

G

V

I

E

L

A

Q

F

L

I

L

A

K

D

D

L

L

R

R

K

S

H

N

G

K

A

I

K

K

I

I

A

A

V

L

V

A

T
x
S

S
x
A

S
|
S

N
x
K

E
x
N

-

G

-

P

-

F

-

L

E

E

K

R

M

M

Q

N

N

-

V

-

Y

-

N

-

A

-

H

-

P

-

E

-

F

L

K

T

G

G

M

Y

V

F

D

D

R

A

I

I

L

A

T

D

G

P

E

A

M

E

F

V

A

A

R

A

S

S

K
|
K

P

P

A

A

P

P

D

D

C

I

F

F

L

I

L

A

G

A

M

A

E

H

I

A

F

V

G

G

A

V

T

A

P

P

E

S

N

E

S

S

Y

I

V

G

F

L

E
|
E

D
|
D

S

S

F

Q

H

A

G

G

L

I

Q

Q

A

A

G

I

M

K

T

D

S

S

G

G

A

A

T

L

V

P

I

I

G

G

L

V

A

G

T

-

N

-

T

-

R

R

E

P

A

E

I

D

T

L

G

G

K

D

A

D

H

I

Y

V

I

I

I

V

D

P

D

D

F

T

S

S

E

H

M

Y

T

T

F

L

E

E

H

F

L

L

active site: D8 (= D12), L9 (≠ F13), D10 (= D14), T16 (= T20), K45 (= K46), S114 (≠ T105), A115 (≠ S106), K145 (= K139), E169 (= E163), D170 (= D164)
binding tetrafluoroaluminate ion: D8 (= D12), L9 (≠ F13), D10 (= D14), S114 (≠ T105), K145 (= K139)
binding 6,7-dideoxy-7-phosphono-beta-D-gluco-heptopyranose: D10 (= D14), G46 (= G47), V47 (vs. gap), R49 (vs. gap), S116 (= S107), K117 (≠ N108), N118 (≠ E109)
binding magnesium ion: D8 (= D12), D10 (= D14), D170 (= D164)

2wf6A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate and aluminium tetrafluoride (see paper)
30% identity, 94% coverage: 8:210/215 of query aligns to 4:212/218 of 2wf6A

query
sites
2wf6A

A

A

A

V

L

L

F

F

D
|
D

F
x
L

D
|
D

G

G

V

V

I

I

M

T

D

D

T
|
T

E

A

T

E

Q

Y

Y

H

T

F

V

R

F

A

W

W

D

-

E

-

Q

-

G

-

R

-

K

K

Y

A

L

L

N

A

E

E

-

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

D

Q

F

F

G

N

R

E

R

Q

I

L

K
|
K

G
|
G

-
x
V

-

S

-
x
R

-

E

-

D

-

S

-

L

Q

Q

T

K

L

I

T

L

Q

D

I

L

Y

A

E

D

K

K

Y

K

F

V

S

S

D

-

L

-

P

A

E

E

A

E

Q

F

Q

K

E

E

I

L

T

A

A

K

G

R

L

K

N

N

-

D

-

N

-

Y

-

V

-

K

I

M

Y

I

E

Q

K

D

S

V

M

S

S

P

Y

A

E

D

Y

V

I

Y

P

P

G

G

V

I

E

L

A

Q

F

L

I

L

A

K

D

D

L

L

R

R

K

S

H

N

G

K

A

I

K

K

I

I

A

A

V

L

V

A

T
x
S

S
x
A

S
|
S

N
x
K

E

N

-

G

-

P

-

F

-

L

E

E

K

R

M

M

Q

N

N

-

V

-

Y

-

N

-

A

-

H

-

P

-

E

-

F

L

K

T

G

G

M

Y

V

F

D

D

R

A

I

I

L

A

T

D

G

P

E

A

M

E

F

V

A

A

R

A

S

S

K
|
K

P

P

A

A

P

P

D

D

C

I

F

F

L

I

L

A

G

A

M

A

E

H

I

A

F

V

G

G

A

V

T

A

P

P

E

S

N

E

S

S

Y

I

V

G

F

L

E
|
E

D
|
D

S

S

F

Q

H

A

G

G

L

I

Q

Q

A

A

G

I

M

K

T

D

S

S

G

G

A

A

T

L

V

P

I

I

G

G

L

V

A

G

T

-

N

-

T

-

R

R

E

P

A

E

I

D

T

L

G

G

K

D

A

D

H

I

Y

V

I

I

I

V

D

P

D

D

F

T

S

S

E

H

M

Y

T

T

F

L

E

E

H

F

L

L

active site: D8 (= D12), L9 (≠ F13), D10 (= D14), T16 (= T20), K45 (= K46), S114 (≠ T105), A115 (≠ S106), K145 (= K139), E169 (= E163), D170 (= D164)
binding tetrafluoroaluminate ion: D8 (= D12), L9 (≠ F13), D10 (= D14), S114 (≠ T105), K145 (= K139)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D14), G46 (= G47), V47 (vs. gap), R49 (vs. gap), S116 (= S107), K117 (≠ N108)
binding magnesium ion: D8 (= D12), D10 (= D14), D170 (= D164)

2wf5A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate and trifluoromagnesate (see paper)
30% identity, 94% coverage: 8:210/215 of query aligns to 4:212/218 of 2wf5A

query
sites
2wf5A

A

A

A

V

L

L

F

F

D
|
D

F
x
L

D
|
D

G

G

V

V

I

I

M

T

D

D

T
|
T

E

A

T

E

Q

Y

Y
x
H

T

F

V

R

F

A

W

W

D

-

E

-

Q

-

G

-

R

-

K

K

Y

A

L

L

N

A

E

E

-

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

D

Q

F

F

G

N

R

E

R

Q

I

L

K
|
K

G
|
G

-
x
V

-

S

-
x
R

-

E

-

D

-

S

-

L

Q

Q

T

K

L

I

T

L

Q

D

I

L

Y

A

E

D

K

K

Y

K

F

V

S

S

D

-

L

-

P

A

E

E

A

E

Q

F

Q

K

E

E

I

L

T

A

A

K

G

R

L

K

N

N

-

D

-

N

-

Y

-

V

-

K

I

M

Y

I

E

Q

K

D

S

V

M

S

S

P

Y

A

E

D

Y

V

I

Y

P

P

G

G

V

I

E

L

A

Q

F

L

I

L

A

K

D

D

L

L

R

R

K

S

H

N

G

K

A

I

K

K

I

I

A

A

V

L

V

A

T
x
S

S
x
A

S
|
S

N

K

E

N

-

G

-

P

-

F

-

L

E

E

K

R

M

M

Q

N

N

-

V

-

Y

-

N

-

A

-

H

-

P

-

E

-

F

L

K

T

G

G

M

Y

V

F

D

D

R

A

I

I

L

A

T

D

G

P

E

A

M

E

F

V

A

A

R

A

S

S

K
|
K

P

P

A

A

P

P

D

D

C

I

F

F

L

I

L

A

G

A

M

A

E

H

I

A

F

V

G

G

A

V

T

A

P

P

E

S

N

E

S

S

Y

I

V

G

F

L

E
|
E

D
|
D

S

S

F

Q

H

A

G

G

L

I

Q

Q

A

A

G

I

M

K

T

D

S

S

G

G

A

A

T

L

V

P

I

I

G

G

L

V

A

G

T

-

N

-

T

-

R

R

E

P

A

E

I

D

T

L

G

G

K

D

A

D

H

I

Y

V

I

I

I

V

D

P

D

D

F

T

S

S

E

H

M

Y

T

T

F

L

E

E

H

F

L

L

active site: D8 (= D12), L9 (≠ F13), D10 (= D14), T16 (= T20), K45 (= K46), S114 (≠ T105), A115 (≠ S106), K145 (= K139), E169 (= E163), D170 (= D164)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D14), H20 (≠ Y24), G46 (= G47), V47 (vs. gap), R49 (vs. gap), A115 (≠ S106), S116 (= S107)
binding magnesium ion: D8 (= D12), D10 (= D14), D170 (= D164)
binding trifluoromagnesate: D8 (= D12), L9 (≠ F13), D10 (= D14), S114 (≠ T105), A115 (≠ S106), K145 (= K139)

2wf9A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate, and beryllium trifluoride, crystal form 2 (see paper)
30% identity, 94% coverage: 8:210/215 of query aligns to 4:212/221 of 2wf9A

query
sites
2wf9A

A

A

A

V

L

L

F

F

D
|
D

F
x
L

D
|
D

G

G

V

V

I

I

M

T

D

D

T
|
T

E

A

T

E

Q

Y

Y
x
H

T

F

V

R

F

A

W

W

D

-

E

-

Q

-

G

-

R

-

K

K

Y

A

L

L

N

A

E

E

-

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

D

Q

F

F

G

N

R

E

R

Q

I

L

K
|
K

G
|
G

-
x
V

-

S

-
x
R

-

E

-

D

-

S

-

L

Q

Q

T

K

L

I

T

L

Q

D

I

L

Y

A

E

D

K

K

Y

K

F

V

S

S

D

-

L

-

P

A

E

E

A

E

Q

F

Q

K

E

E

I

L

T

A

A

K

G

R

L

K

N

N

-

D

-

N

-

Y

-

V

-

K

I

M

Y

I

E

Q

K

D

S

V

M

S

S

P

Y

A

E

D

Y

V

I

Y

P

P

G

G

V

I

E

L

A

Q

F

L

I

L

A

K

D

D

L

L

R

R

K

S

H

N

G

K

A

I

K

K

I

I

A

A

V

L

V

A

T
x
S

S
x
A

S
|
S

N
x
K

E
x
N

-

G

-

P

-

F

-

L

E

E

K

R

M

M

Q

N

N

-

V

-

Y

-

N

-

A

-

H

-

P

-

E

-

F

L

K

T

G

G

M

Y

V

F

D

D

R

A

I

I

L

A

T

D

G

P

E

A

M

E

F

V

A

A

R

A

S

S

K
|
K

P

P

A

A

P

P

D

D

C

I

F

F

L

I

L

A

G

A

M

A

E

H

I

A

F

V

G

G

A

V

T

A

P

P

E

S

N

E

S

S

Y

I

V

G

F

L

E
|
E

D
|
D

S

S

F

Q

H

A

G

G

L

I

Q

Q

A

A

G

I

M

K

T

D

S

S

G

G

A

A

T

L

V

P

I

I

G

G

L

V

A

G

T

-

N

-

T

-

R

R

E

P

A

E

I

D

T

L

G

G

K

D

A

D

H

I

Y

V

I

I

I

V

D

P

D

D

F

T

S

S

E

H

M

Y

T

T

F

L

E

E

H

F

L

L

active site: D8 (= D12), L9 (≠ F13), D10 (= D14), T16 (= T20), K45 (= K46), S114 (≠ T105), A115 (≠ S106), K145 (= K139), E169 (= E163), D170 (= D164)
binding beryllium trifluoride ion: D8 (= D12), L9 (≠ F13), D10 (= D14), S114 (≠ T105), A115 (≠ S106), K145 (= K139)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D14), H20 (≠ Y24), G46 (= G47), V47 (vs. gap), R49 (vs. gap), S116 (= S107), K117 (≠ N108), N118 (≠ E109)
binding 6-O-phosphono-alpha-D-glucopyranose: D10 (= D14), H20 (≠ Y24), G46 (= G47), V47 (vs. gap), R49 (vs. gap), A115 (≠ S106), S116 (= S107), K117 (≠ N108), N118 (≠ E109)
binding magnesium ion: D8 (= D12), D10 (= D14), D170 (= D164)

1o03A Structure of pentavalent phosphorous intermediate of an enzyme catalyzed phosphoryl transfer reaction observed on cocrystallization with glucose 6-phosphate (see paper)
30% identity, 94% coverage: 8:210/215 of query aligns to 4:212/221 of 1o03A

query
sites
1o03A

A

A

A

V

L

L

F

F

D
|
D

F
x
L

D
|
D

G

G

V

V

I

I

M

T

D

D

T
|
T

E

A

T

E

Q

Y

Y
x
H

T

F

V

R

F

A

W

W

D

-

E

-

Q

-

G

-

R

-

K

K

Y

A

L

L

N

A

E

E

-

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

D

Q

F

F

G

N

R

E

R

Q

I

L

K
|
K

G
|
G

-
x
V

-

S

-
x
R

-

E

-

D

-

S

-

L

Q

Q

T

K

L

I

T

L

Q

D

I

L

Y

A

E

D

K

K

Y

K

F

V

S

S

D

-

L

-

P

A

E

E

A

E

Q

F

Q

K

E

E

I

L

T

A

A

K

G

R

L

K

N

N

-

D

-

N

-

Y

-

V

-

K

I

M

Y

I

E

Q

K

D

S

V

M

S

S

P

Y

A

E

D

Y

V

I

Y

P

P

G

G

V

I

E

L

A

Q

F

L

I

L

A

K

D

D

L

L

R

R

K

S

H

N

G

K

A

I

K

K

I

I

A

A

V

L

V

A

T
x
S

S
x
A

S
|
S

N
x
K

E

N

-

G

-

P

-

F

-

L

E

E

K

R

M

M

Q

N

N

-

V

-

Y

-

N

-

A

-

H

-

P

-

E

-

F

L

K

T

G

G

M

Y

V

F

D

D

R

A

I

I

L

A

T

D

G

P

E

A

M

E

F

V

A

A

R

A

S

S

K
|
K

P

P

A

A

P

P

D

D

C

I

F

F

L

I

L

A

G

A

M

A

E

H

I

A

F

V

G

G

A

V

T

A

P

P

E

S

N

E

S

S

Y

I

V

G

F

L

E
|
E

D
|
D

S

S

F

Q

H

A

G

G

L

I

Q

Q

A

A

G

I

M

K

T

D

S

S

G

G

A

A

T

L

V

P

I

I

G

G

L

V

A

G

T

-

N

-

T

-

R

R

E

P

A

E

I

D

T

L

G

G

K

D

A

D

H

I

Y

V

I

I

I

V

D

P

D

D

F

T

S

S

E

H

M

Y

T

T

F

L

E

E

H

F

L

L

active site: D8 (= D12), L9 (≠ F13), D10 (= D14), T16 (= T20), K45 (= K46), S114 (≠ T105), A115 (≠ S106), K145 (= K139), E169 (= E163), D170 (= D164)
binding 1,6-di-O-phosphono-alpha-D-glucopyranose: D8 (= D12), L9 (≠ F13), D10 (= D14), H20 (≠ Y24), G46 (= G47), V47 (vs. gap), R49 (vs. gap), S114 (≠ T105), A115 (≠ S106), S116 (= S107), K117 (≠ N108), K145 (= K139)
binding magnesium ion: D8 (= D12), D10 (= D14), D170 (= D164)

1lvhA The structure of phosphorylated beta-phosphoglucomutase from lactoccocus lactis to 2.3 angstrom resolution (see paper)
30% identity, 94% coverage: 8:210/215 of query aligns to 4:212/221 of 1lvhA

query
sites
1lvhA

A

A

A

V

L

L

F

F

D
|
D

F
x
L

D
|
D

G

G

V

V

I

I

M

T

D

D

T
|
T

E

A

T

E

Q

Y

Y

H

T

F

V

R

F

A

W

W

D

-

E

-

Q

-

G

-

R

-

K

K

Y

A

L

L

N

A

E

E

-

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

D

Q

F

F

G

N

R

E

R

Q

I

L

K
|
K

G

G

-

V

-

S

-

R

-

E

-

D

-

S

-

L

Q

Q

T

K

L

I

T

L

Q

D

I

L

Y

A

E

D

K

K

Y

K

F

V

S

S

D

-

L

-

P

A

E

E

A

E

Q

F

Q

K

E

E

I

L

T

A

A

K

G

R

L

K

N

N

-

D

-

N

-

Y

-

V

-

K

I

M

Y

I

E

Q

K

D

S

V

M

S

S

P

Y

A

E

D

Y

V

I

Y

P

P

G

G

V

I

E

L

A

Q

F

L

I

L

A

K

D

D

L

L

R

R

K

S

H

N

G

K

A

I

K

K

I

I

A

A

V

L

V

A

T
x
S

S
x
A

S

S

N

K

E

N

-

G

-

P

-

F

-

L

E

E

K

R

M

M

Q

N

N

-

V

-

Y

-

N

-

A

-

H

-

P

-

E

-

F

L

K

T

G

G

M

Y

V

F

D

D

R

A

I

I

L

A

T

D

G

P

E

A

M

E

F

V

A

A

R

A

S

S

K
|
K

P

P

A

A

P

P

D

D

C

I

F

F

L

I

L

A

G

A

M

A

E

H

I

A

F

V

G

G

A

V

T

A

P

P

E

S

N

E

S

S

Y

I

V

G

F

L

E
|
E

D
|
D

S

S

F

Q

H

A

G

G

L

I

Q

Q

A

A

G

I

M

K

T

D

S

S

G

G

A

A

T

L

V

P

I

I

G

G

L

V

A

G

T

-

N

-

T

-

R

R

E

P

A

E

I

D

T

L

G

G

K

D

A

D

H

I

Y

V

I

I

I

V

D

P

D

D

F

T

S

S

E

H

M

Y

T

T

F

L

E

E

H

F

L

L

active site: D8 (= D12), L9 (≠ F13), D10 (= D14), T16 (= T20), K45 (= K46), S114 (≠ T105), A115 (≠ S106), K145 (= K139), E169 (= E163), D170 (= D164)
binding magnesium ion: D8 (= D12), D10 (= D14), D170 (= D164)

P71447 Beta-phosphoglucomutase; Beta-PGM; EC 5.4.2.6 from Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) (see 3 papers)
30% identity, 94% coverage: 8:210/215 of query aligns to 4:212/221 of P71447

query
sites
P71447

A

A

A

V

L

L

F

F

D
|
D

F

L

D
|
D

G

G

V

V

I

I

M

T

D

D

T
|
T

E

A

T

E

Q

Y

Y
x
H

T

F

V

R

F

A

W

W

D

-

E

-

Q

-

G

-

R

-

K

K

Y

A

L

L

N

A

E

E

-

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

D

Q

F

F

G

N

R

E

R

Q

I

L

K
|
K

G
|
G

-

V

-

S

-
x
R

-

E

-

D

-
x
S

-

L

Q

Q

T

K

L

I

T

L

Q

D

I

L

Y

A

E

D

K

K

Y

K

F

V

S

S

D

-

L

-

P

A

E

E

A

E

Q

F

Q

K

E

E

I

L

T

A

A

K

G

R

L
x
K

N

N

-

D

-

N

-

Y

-

V

-

K

I

M

Y

I

E

Q

K

D

S

V

M

S

S

P

Y

A

E

D

Y

V

I

Y

P

P

G

G

V

I

E

L

A

Q

F

L

I

L

A

K

D

D

L

L

R

R

K

S

H

N

G

K

A

I

K

K

I

I

A

A

V

L

V

A

T

S

S

A

S

S

N

K

E

N

-

G

-

P

-

F

-

L

E

E

K

K

M

M

Q

N

N

-

V

-

Y

-

N

-

A

-

H

-

P

-

E

-

F

L

K

T

G

G

M

Y

V

F

D

D

R

A

I

I

L

A

T

D

G

P

E

A

M

E

F

V

A

A

R

A

S

S

K

K

P

P

A

A

P

P

D

D

C

I

F

F

L

I

L

A

G

A

M

A

E

H

I

A

F

V

G

G

A

V

T

A

P

P

E

S

N

E

S

S

Y

I

V

G

F

L

E

E

D
|
D

S

S

F

Q

H

A

G

G

L

I

Q

Q

A

A

G

I

M

K

T

D

S

S

G

G

A

A

T

L

V

P

I

I

G

G

L

V

A

G

T

-

N

-

T

-

R

R

E

P

A

E

I

D

T

L

G

G

K

D

A

D

H

I

Y

V

I

I

I

V

D

P

D

D

F

T

S

S

E

Y

M

Y

T

T

F

L

E

E

H

F

L

L

D8 (= D12) modified: 4-aspartylphosphate; mutation to A: Inactive.; mutation to E: Inactive.
D10 (= D14) mutation to A: Inactive.; mutation to E: Inactive.; mutation to N: Inactive.; mutation to S: Inactive.
T16 (= T20) mutation to P: 500-fold reduction in the rate constant for Asp-8 phosphorylation by beta-G1,6bisP. 6,700-fold reduction in the apparent rate constant for cycling of the phosphorylated enzyme to convert beta-G1P to G6P. 13-fold increase in the estimated rate constant for phosphoryl transfer from the phospho-Asp8 to water.
H20 (≠ Y24) mutation to A: Impairs Asp-8 phosphorylation by beta-G1,6bisP and phosphoryl transfer from the phospho-Asp8 to the substrate beta-G1P.; mutation to N: 300-fold reduction in the conversion of beta-G1P to G6P in the presence of beta-G1,6bisP.; mutation to Q: 8-fold reduction in the conversion of beta-G1P to G6P in the presence of beta-G1,6bisP.
K45 (= K46) mutation to A: 20'000-fold decrease in kcat/KM.
G46 (= G47) mutation to A: 1'000'000-fold decrease in kcat/KM.; mutation to P: 100'000-fold decrease in kcat/KM.; mutation to V: 10'000-fold decrease in kcat/KM.
R49 (vs. gap) mutation to K: 1'000'000-fold decrease in kcat/KM.
S52 (vs. gap) mutation to A: Wild-type activity.
K76 (≠ L72) mutation to A: 100-fold reduction in the conversion of beta-G1P to G6P in the presence of beta-G1,6bisP.
D170 (= D164) mutation to A: Impaired, but active with an increase in the affinity for G1P.

6qzgA Beta-glucose 1,6-bisphosphonate bound to wild type beta- phosphoglucomutse in an open conformation.
30% identity, 94% coverage: 8:210/215 of query aligns to 4:212/219 of 6qzgA

query
sites
6qzgA

A

A

A

V

L

L

F

F

D
|
D

F
x
L

D
|
D

G

G

V

V

I

I

M

T

D

D

T

T

E

A

T

E

Q

Y

Y
x
H

T

F

V

R

F

A

W

W

D

-

E

-

Q

-

G

-

R

-

K

K

Y

A

L

L

N

A

E

E

-

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

D

Q

F

F

G

N

R

E

R

Q

I

L

K

K

G
|
G

-

V

-

S

-

R

-

E

-

D

-

S

-

L

Q

Q

T

K

L

I

T

L

Q

D

I

L

Y

A

E

D

K

K

Y

K

F

V

S

S

D

-

L

-

P

A

E

E

A

E

Q

F

Q

K

E

E

I

L

T

A

A

K

G

R

L

K

N

N

-

D

-

N

-

Y

-

V

-

K

I

M

Y

I

E

Q

K

D

S

V

M

S

S

P

Y

A

E

D

Y

V

I

Y

P

P

G

G

V

I

E

L

A

Q

F

L

I

L

A

K

D

D

L

L

R

R

K

S

H

N

G

K

A

I

K

K

I

I

A

A

V

L

V

A

T
x
S

S
x
A

S
|
S

N
x
K

E

N

-

G

-

P

-

F

-

L

E

E

K

R

M

M

Q

N

N

-

V

-

Y

-

N

-

A

-

H

-

P

-

E

-

F

L

K

T

G

G

M

Y

V

F

D

D

R

A

I

I

L

A

T

D

G

P

E

A

M

E

F

V

A

A

R

A

S

S

K
|
K

P

P

A

A

P

P

D

D

C

I

F

F

L

I

L

A

G

A

M

A

E

H

I

A

F

V

G

G

A

V

T

A

P

P

E

S

N

E

S

S

Y

I

V

G

F

L

E

E

D
|
D

S

S

F

Q

H

A

G

G

L

I

Q

Q

A

A

G

I

M

K

T

D

S

S

G

G

A

A

T

L

V

P

I

I

G

G

L

V

A

G

T

-

N

-

T

-

R

R

E

P

A

E

I

D

T

L

G

G

K

D

A

D

H

I

Y

V

I

I

I

V

D

P

D

D

F

T

S

S

E

H

M

Y

T

T

F

L

E

E

H

F

L

L

binding 3,7-anhydro-1,2,8-trideoxy-1,8-diphosphono-D-glycero-D-gulo-octitol: D8 (= D12), L9 (≠ F13), D10 (= D14), H20 (≠ Y24), G46 (= G47), S114 (≠ T105), A115 (≠ S106), S116 (= S107), K117 (≠ N108), K145 (= K139)
binding magnesium ion: D8 (= D12), D10 (= D14), D170 (= D164)

1z4nA Structure of beta-phosphoglucomutase with inhibitor bound alpha- galactose 1-phosphate cocrystallized with fluoride (see paper)
30% identity, 94% coverage: 8:210/215 of query aligns to 4:212/219 of 1z4nA

query
sites
1z4nA

A

A

A

V

L

L

F

F

D
|
D

F
x
L

D
|
D

G

G

V

V

I

I

M

T

D

D

T
|
T

E

A

T

E

Q

Y

Y
x
H

T

F

V

R

F

A

W
|
W

D

-

E

-

Q

-

G

-

R

-

K

K

Y

A

L

L

N

A

E

E

-

I

-

G

-

I

-

N

-

G

-

V

-

D

-

R

D

Q

F

F

G

N

R

E

R

Q

I

L

K
|
K

G

G

-
x
V

-

S

-
x
R

-

E

-

D

-

S

-

L

Q

Q

T

K

L

I

T

L

Q

D

I

L

Y

A

E

D

K

K

Y

K

F

V

S

S

D

-

L

-

P

A

E

E

A

E

Q

F

Q

K

E

E

I

L

T

A

A

K

G

R

L

K

N

N

-

D

-

N

-

Y

-

V

-

K

I

M

Y

I

E

Q

K

D

S

V

M

S

S

P

Y

A

E

D

Y

V

I

Y

P

P

G

G

V

I

E

L

A

Q

F

L

I

L

A

K

D

D

L

L

R

R

K

S

H

N

G

K

A

I

K

K

I

I

A

A

V

L

V

A

T
x
S

S
x
A

S
|
S

N
x
K

E
x
N

-

G

-

P

-

F

-

L

E

E

K

R

M

M

Q

N

N

-

V

-

Y

-

N

-

A

-

H

-

P

-

E

-

F

L

K

T

G

G

M

Y

V

F

D

D

R

A

I

I

L

A

T

D

G

P

E

A

M

E

F

V

A

A

R

A

S

S

K
|
K

P

P

A

A

P

P

D

D

C

I

F

F

L

I

L

A

G

A

M

A

E

H

I

A

F

V

G

G

A

V

T

A

P

P

E

S

N

E

S

S

Y

I

V

G

F

L

E
|
E

D
|
D

S

S

F

Q

H

A

G

G

L

I

Q

Q

A

A

G

I

M

K

T

D

S

S

G

G

A

A

T

L

V

P

I

I

G

G

L

V

A

G

T

-

N

-

T

-

R

R

E

P

A

E

I

D

T

L

G

G

K

D

A

D

H

I

Y

V

I

I

I

V

D

P

D

D

F

T

S

S

E

H

M

Y

T

T

F

L

E

E

H

F

L

L

active site: D8 (= D12), L9 (≠ F13), D10 (= D14), T16 (= T20), K45 (= K46), S114 (≠ T105), A115 (≠ S106), K145 (= K139), E169 (= E163), D170 (= D164)
binding 1-O-phosphono-alpha-D-galactopyranose: H20 (≠ Y24), W24 (= W28), V47 (vs. gap), R49 (vs. gap), S116 (= S107), K117 (≠ N108), N118 (≠ E109)
binding magnesium ion: D8 (= D12), D10 (= D14), E169 (= E163), D170 (= D164)

Query Sequence

>350225 FitnessBrowser__Btheta:350225
MDTTKTIAALFDFDGVIMDTETQYTVFWDEQGRKYLNEEDFGRRIKGQTLTQIYEKYFSD
LPEAQQEITAGLNIYEKSMSYEYIPGVEAFIADLRKHGAKIAVVTSSNEEKMQNVYNAHP
EFKGMVDRILTGEMFARSKPAPDCFLLGMEIFGATPENSYVFEDSFHGLQAGMTSGATVI
GLATTNTREAITGKAHYIIDDFSEMTFEHLLALHR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory