SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 350423 FitnessBrowser__Btheta:350423 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9X1K9 4-hydroxy-tetrahydrodipicolinate synthase; HTPA synthase; EC 4.3.3.7 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
45% identity, 97% coverage: 6:292/297 of query aligns to 2:290/294 of Q9X1K9

query
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Q9X1K9

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DID 166:168 (≠ NIT 171:173) mutation to AAA: Exists as a monomer in solution. Decreased activity and substrate affinity. Reduced thermal stability.

1o5kA Crystal structure of dihydrodipicolinate synthase (tm1521) from thermotoga maritima at 1.80 a resolution
45% identity, 97% coverage: 6:292/297 of query aligns to 3:291/295 of 1o5kA

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1o5kA

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active site: T44 (= T48), Y107 (= Y111), Y133 (= Y137), R138 (= R142), K162 (= K166), I207 (= I208)
binding calcium ion: E65 (≠ D69), D68 (≠ N72)

4dxvA Crystal structure of dihydrodipicolinate synthase from acinetobacter baumannii complexed with mg and cl ions at 1.80 a resolution
41% identity, 98% coverage: 6:295/297 of query aligns to 2:290/291 of 4dxvA

query
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active site: T44 (= T48), Y107 (= Y111), Y133 (= Y137), R138 (= R142), K161 (= K166), I203 (= I208)
binding magnesium ion: T104 (≠ V108), N134 (= N138), V135 (= V139), P136 (= P140), T139 (= T143)

3u8gA Crystal structure of the complex of dihydrodipicolinate synthase from acinetobacter baumannii with oxalic acid at 1.80 a resolution
41% identity, 98% coverage: 6:295/297 of query aligns to 2:290/291 of 3u8gA

query
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3u8gA

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active site: T44 (= T48), Y107 (= Y111), Y133 (= Y137), R138 (= R142), K161 (= K166), I203 (= I208)
binding oxalate ion: A8 (= A12), G43 (= G47), T44 (= T48), T45 (= T49), L101 (= L105), Y133 (= Y137), K161 (= K166)

3tdfA Crystal structure of the complex of dihydrodipicolinate synthase from acinetobacter baumannii with 2-ketobutanoic acid at 1.99 a resolution
41% identity, 98% coverage: 6:295/297 of query aligns to 2:290/291 of 3tdfA

query
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3tdfA

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V

A

N

N

G

K

R

R

I

I

P

P

I

I

L

I

L

A

G

G

V

T

G

G

G

A

N

N

N

S

T

T

R

R

A

E

I

A

V

I

E

E

T

L

L

T

K

K

N

A

D

A

D

K

F

D

T

L

G

G

V

A

D

D

A

A

I

A

L

L

S

L

V

V

V

T

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

S

T

Q

Q

E

E

G

G

I

L

Y

Y

Q

Q

H

H

Y

Y

K

K

A

A

I

I

S

A

E

E

A

A

T

V

E

E

L

L

P

P

I

L

V

I

L

L

Y
|
Y

N

N

V

V

P

P

G

G

R
|
R

T

T

G

G

V

V

N

D

M

L

T

S

A

N

E

D

T

T

T

A

L

V

R

R

I

L

A

A

R

E

N

-

F

I

S

P

N

N

V

I

I

V

A

G

I

I

K
|
K

E

D

A

A

S

T

G

G

N

D

I

V

T

P

Q

R

M

G

D

K

D

A

I

L

I

I

K

D

N

A

K

L

P

N

D

G

N

K

F

M

N

A

V

V

I

Y

S

S

G

G

D

D

G

E

I

T

T

A

F

W

P

E

L

L

I

M

T

L

L

L

G

G

A

A

V

D

G

G

V

N

I
|
I

S

S

V

V

I

T

G

A

N

N

A

I

F

A

P

P

R

K

E

A

F

M

S

S

R

E

M

V

T

C

R

A

L

V

A

A

L

I

Q

A

G

K

D

D

F

E

A

Q

N

Q

A

A

L

K

T

T

I

L

H

N

R

K

F

I

T

A

E

N

L

L

F

H

D

N

L

I

L

L

F

F

V

C

D

E

G

S

N

N

P

P

A

I

G

P

V

V

K

K

S

W

M

A

L

L

N

H

A

E

M

M

G

G

M

L

I

I

E

D

N

T

K

G

L

I

R

R

L

L

P

P

L

L

V

T

P

P

T

L

R

A

I

E

T

Q

T

Y

F

R

E

E

A

P

I

L

R

R

K

N

V

A

L

L

N

K

E

D

L

A

N

G

I

I

active site: T44 (= T48), Y107 (= Y111), Y133 (= Y137), R138 (= R142), K161 (= K166), I203 (= I208)
binding 2-ketobutyric acid: A8 (= A12), T44 (= T48), T45 (= T49), Y133 (= Y137), K161 (= K166), I203 (= I208)

3tceA Crystal structure of the complex of dihydrodipicolinate synthase from acinetobacter baumannii with 5-hydroxylysine at 2.6 a resolution
41% identity, 98% coverage: 6:295/297 of query aligns to 2:290/291 of 3tceA

query
sites
3tceA

L

I

K

Q

G

G

M

S

G

I

V

V

A

A

L

I

I

V

T

T

P

P

F

M

K

L

E

K

D

D

E

G

S

G

V

V

D

D

Y

W

D

K

A

S

L

L

M

E

R

K

L

L

V

V

D

E

Y

W

L

H

L

I

Q

E

N

Q

N

G

A

T

D

N

F

S

L

I

C

V

V

A

L

V

G

G

T
|
T

T

T

A

G

E

E

T

A

P
x
S

T

T

L
|
L

T

S

E

M

E

E

K
x
H

K

T

T

Q

I

V

K

I

K

K

M

E

V

I

I

I

D

R

R

V

V

A

N

N

G

K

R

R

I

I

P

P

I

I

L

I

L

A

G

G

V

T

G

G

G

A

N

N

N

S

T

T

R

R

A

E

I

A

V

I

E

E

T

L

L

T

K

K

N

A

D

A

D

K

F

D

T

L

G

G

V

A

D

D

A

A

I

A

L

L

S

L

V

V

V

T

P

P

Y
|
Y

N

N

K

K

P

P

S

T

Q

Q

E

E

G

G

I

L

Y

Y

Q

Q

H

H

Y

Y

K

K

A

A

I

I

S

A

E

E

A

A

T

V

E

E

L

L

P

P

I

L

V

I

L

L

Y
|
Y

N

N

V

V

P

P

G

G

R
|
R

T

T

G

G

V

V

N

D

M

L

T

S

A

N

E

D

T

T

T

A

L

V

R

R

I

L

A

A

R

E

N

-

F

I

S

P

N

N

V

I

I

V

A

G

I

I

K
|
K

E

D

A

A

S

T

G

G

N

D

I

V

T

P

Q

R

M

G

D

K

D

A

I

L

I

I

K

D

N

A

K

L

P

N

D

G

N

K

F

M

N

A

V

V

I

Y

S

S

G

G

D

D

G

E

I

T

T

A

F

W

P

E

L

L

I

M

T

L

L

L

G

G

A

A

V

D

G

G

V

N

I
|
I

S

S

V

V

I

T

G

A

N

N

A

I

F

A

P

P

R

K

E

A

F

M

S

S

R

E

M

V

T

C

R

A

L

V

A

A

L

I

Q

A

G

K

D

D

F

E

A

Q

N

Q

A

A

L

K

T

T

I

L

H

N

R

K

F

I

T

A

E

N

L

L

F

H

D

N

L

I

L

L

F

F

V

C

D

E

G

S

N

N

P

P

A

I

G

P

V

V

K

K

S

W

M

A

L

L

N

H

A

E

M

M

G

G

M

L

I

I

E

D

N

T

K

G

L

I

R

R

L

L

P

P

L

L

V

T

P

P

T

L

R

A

I

E

T

Q

T

Y

F

R

E

E

A

P

I

L

R

R

K

N

V

A

L

L

N

K

E

D

L

A

N

G

I

I

active site: T44 (= T48), Y107 (= Y111), Y133 (= Y137), R138 (= R142), K161 (= K166), I203 (= I208)
binding 5-hydroxylysine: S49 (≠ P53), L51 (= L55), H56 (≠ K60), Y106 (= Y110)

3rk8A Crystal structure of the chloride inhibited dihydrodipicolinate synthase from acinetobacter baumannii complexed with pyruvate at 1.8 a resolution
41% identity, 98% coverage: 6:295/297 of query aligns to 2:290/291 of 3rk8A

query
sites
3rk8A

L

I

K

Q

G

G

M

S

G

I

V

V

A

A

L

I

I

V

T

T

P

P

F

M

K

L

E

K

D

D

E

G

S

G

V

V

D

D

Y

W

D

K

A

S

L

L

M

E

R

K

L

L

V

V

D

E

Y

W

L

H

L

I

Q

E

N

Q

N

G

A

T

D

N

F

S

L

I

C

V

V

A

L

V

G

G

T
|
T

T

T

A

G

E

E

T

A

P

S

T

T

L

L

T

S

E

M

E

E

K

H

K

T

T

Q

I

V

K

I

K

K

M

E

V

I

I

I

D

R

R

V

V

A

N

N

G

K

R

R

I

I

P

P

I

I

L

I

L

A

G

G

V

T

G

G

G

A

N

N

N

S

T

T

R

R

A

E

I

A

V

I

E

E

T

L

L

T

K

K

N

A

D

A

D

K

F

D

T

L

G

G

V

A

D

D

A

A

I

A

L

L

S

L

V

V

V

T

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

S

T

Q

Q

E

E

G

G

I

L

Y

Y

Q

Q

H

H

Y

Y

K

K

A

A

I

I

S

A

E

E

A

A

T

V

E

E

L

L

P

P

I

L

V

I

L

L

Y
|
Y

N

N

V

V

P

P

G

G

R
|
R

T

T

G

G

V

V

N

D

M

L

T

S

A

N

E

D

T

T

T

A

L

V

R

R

I

L

A

A

R

E

N

-

F

I

S

P

N

N

V

I

I

V

A

G

I

I

K
|
K

E

D

A

A

S

T

G

G

N

D

I

V

T

P

Q

R

M

G

D

K

D

A

I

L

I

I

K

D

N

A

K

L

P

N

D

G

N

K

F

M

N

A

V

V

I

Y

S

S

G
|
G

D

D

G

E

I

T

T

A

F

W

P

E

L

L

I

M

T

L

L

L

G

G

A

A

V

D

G

G

V

N

I
|
I

S

S

V

V

I

T

G

A

N

N

A

I

F

A

P

P

R

K

E

A

F

M

S

S

R

E

M

V

T

C

R

A

L

V

A

A

L

I

Q

A

G

K

D

D

F

E

A

Q

N

Q

A

A

L

K

T

T

I

L

H

N

R

K

F

I

T

A

E

N

L

L

F

H

D

N

L

I

L

L

F
|
F

V

C

D

E

G

S

N

N

P

P

A

I

G

P

V

V

K

K

S

W

M

A

L

L

N

H

A

E

M

M

G

G

M

L

I

I

E

D

N

T

K

G

L

I

R

R

L

L

P

P

L

L

V

T

P

P

T

L

R

A

I

E

T

Q

T

Y

F

R

E

E

A

P

I

L

R

R

K

N

V

A

L

L

N

K

E

D

L

A

N

G

I

I

active site: T44 (= T48), Y107 (= Y111), Y133 (= Y137), R138 (= R142), K161 (= K166), I203 (= I208)
binding pyruvic acid: R138 (= R142), K161 (= K166), G186 (= G191), F244 (= F249)

3pueB Crystal structure of the complex of dhydrodipicolinate synthase from acinetobacter baumannii with lysine at 2.6a resolution
41% identity, 98% coverage: 6:295/297 of query aligns to 2:290/291 of 3pueB

query
sites
3pueB

L

I

K

Q

G

G

M

S

G

I

V

V

A

A

L

I

I

V

T

T

P

P

F

M

K

L

E

K

D

D

E

G

S

G

V

V

D

D

Y

W

D

K

A

S

L

L

M

E

R

K

L

L

V

V

D

E

Y

W

L

H

L

I

Q

E

N

Q

N

G

A

T

D

N

F

S

L

I

C

V

V

A

L

V

G

G

T
|
T

T

T

A

G

E

E

T

A

P

S

T

T

L

L

T

S

E

M

E

E

K

H

K

T

T

Q

I

V

K

I

K

K

M

E

V

I

I

I

D

R

R

V

V

A

N

N

G

K

R

R

I

I

P

P

I

I

L

I

L

A

G

G

V

T

G

G

G

A

N

N

N

S

T

T

R

R

A

E

I

A

V

I

E

E

T

L

L

T

K

K

N

A

D

A

D

K

F

D

T

L

G

G

V

A

D

D

A

A

I

A

L

L

S

L

V

V

V

T

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

S

T

Q

Q

E

E

G

G

I

L

Y

Y

Q

Q

H

H

Y

Y

K

K

A

A

I

I

S

A

E

E

A

A

T

V

E

E

L

L

P

P

I

L

V

I

L

L

Y
|
Y

N

N

V

V

P

P

G

G

R
|
R

T

T

G

G

V

V

N

D

M

L

T

S

A

N

E

D

T

T

T

A

L

V

R

R

I

L

A

A

R

E

N

-

F

I

S

P

N

N

V

I

I

V

A

G

I

I

K
|
K

E

D

A

A

S

T

G

G

N

D

I

V

T

P

Q

R

M

G

D

K

D

A

I

L

I

I

K

D

N

A

K

L

P

N

D

G

N

K

F

M

N

A

V

V

I

Y

S

S

G

G

D

D

G

E

I

T

T

A

F

W

P

E

L

L

I

M

T

L

L

L

G

G

A

A

V

D

G

G

V

N

I
|
I

S

S

V

V

I

T

G

A

N

N

A

I

F

A

P

P

R

K

E

A

F

M

S

S

R

E

M

V

T

C

R

A

L

V

A

A

L

I

Q

A

G

K

D

D

F

E

A

Q

N

Q

A

A

L

K

T

T

I

L

H

N

R

K

F

I

T

A

E

N

L

L

F

H

D

N

L

I

L

L

F
|
F

V

C

D

E

G

S

N
|
N

P

P

A

I

G

P

V

V

K

K

S

W

M

A

L

L

N

H

A

E

M

M

G

G

M

L

I

I

E

D

N

T

K

G

L

I

R

R

L

L

P

P

L

L

V

T

P

P

T

L

R

A

I

E

T

Q

T

Y

F

R

E

E

A

P

I

L

R

R

K

N

V

A

L

L

N

K

E

D

L

A

N

G

I

I

active site: T44 (= T48), Y107 (= Y111), Y133 (= Y137), R138 (= R142), K161 (= K166), I203 (= I208)
binding lysine: R138 (= R142), F244 (= F249), N248 (= N253)

4fhaA Structure of dihydrodipicolinate synthase from streptococcus pneumoniae,bound to pyruvate and lysine
43% identity, 94% coverage: 12:290/297 of query aligns to 13:290/307 of 4fhaA

query
sites
4fhaA

A

A

L

F

I

I

T

T

P

P

F

F

K

H

E

E

D

D

E

G

S

S

V

I

D

N

Y

F

D

D

A

A

L

I

M

P

R

A

L

L

V

I

D

E

Y

H

L

L

Q

A

N

H

A

T

D

D

F

G

L

I

C

L

V

L

L

A

G

G

T

T

A

A

E

E

T
x
S

P
|
P

T

T

L
|
L

T

T

E
x
H

E

D

E

E

K
x
E

K

L

T

E

I

L

K

F

K

A

M

A

V

V

I

Q

D

K

R

V

V

V

N

N

G

G

R

R

I

V

P

P

I

L

L

I

L

A

G

G

V

V

G

G

G

T

N

N

N

D

T

T

R

R

A

D

I

S

V

I

E

E

T

F

L

V

K

K

N

E

-

V

D

A

D

E

F

F

T

G

G

G

V

F

D

A

A

A

I

G

L

L

S

A

V

I

V

V

P

P

Y
|
Y

Y

Y

N

N

K

K

P

P

S

S

Q

Q

E

E

G

G

I

M

Y

Y

Q

Q

H

H

Y

F

K

K

A

A

I

I

S

A

E

D

A

A

T

S

E

D

L

L

P

P

I

I

V

I

L

I

Y

Y

N

N

V

I

P

P

G

G

R

R

T

V

G

V

V

V

N

E

M

L

T

T

A

P

E

E

T

T

T

M

L

L

R

R

I

L

A

A

R

-

N

D

F

H

S

P

N

N

V

I

I

I

A

G

I

V

K

K

E

E

A

C

S

T

G

-

N

S

I

L

T

A

Q

N

M

M

D

A

D

Y

I

L

I

I

K

E

N

H

K

K

P

P

D

E

N

E

F

F

N

L

V

I

I

Y

S

T

G

G

D

E

D

D

G

G

I

D

T

A

F

F

P

H

L

A

I

M

T

N

L

L

G

G

A

A

V

D

G

G

V

V

I

I

S

S

V

V

I

A

G

S

N

H

A

T

F

N

P

G

R

D

E

E

F

M

S

H

R

E

M

M

T

F

R

T

L

A

A

I

L

A

Q

E

G

S

D

D

F

M

A

K

N

K

A

A

L

A

T

A

I

I

H

Q

H

R

R

K

F

F

T

I

E

P

L

K

F

V

D

N

L

A

L

L

F

F

V

S

D

Y

G

P

N

S

P

P

A

A

G

P

V

V

K

K

S

A

M

I

L

L

N

N

A

Y

M

M

G

G

M

F

I

E

E

A

N

G

K

P

L

T

R

R

L

L

P

P

L

L

V

V

P

P

T

A

R

P

I

E

T

E

T

D

F

V

E

K

A

R

I

I

R

I

K

K

V

V

L

V

binding lysine: S53 (≠ T52), P54 (= P53), L56 (= L55), H58 (≠ E57), E61 (≠ K60), Y112 (= Y110)

2atsA Dihydrodipicolinate synthase co-crystallised with (s)-lysine
39% identity, 96% coverage: 11:295/297 of query aligns to 7:291/292 of 2atsA

query
sites
2atsA

V

V

A

A

L

I

I

V

T

T

P

P

F

M

K

D

E

E

D

K

E

G

S

N

V

V

D

C

Y

R

D

A

A

S

L

L

M

K

R

K

L

L

V

I

D

D

Y

Y

L

H

L

V

Q

A

N

S

N

G

A

T

D

S

F

A

L

I

C

V

V

S

L

V

G

G

T
|
T

T

T

A

G

E

E

T

S

P
x
A

T

T

L
|
L

T

N

E
x
H

E

D

E

E

K
x
H

K

A

T

D

I

V

K

V

K

M

M

M

V

T

I

L

D

D

R

L

V

A

N

D

G

G

R

R

I

I

P

P

I

V

L

I

L

A

G

G

V

T

G

G

G

A

N
|
N

N

A

T

T

R

A

A
x
E

I

A

V

I

E

S

T

L

L

T

K

Q

N

R

D

F

D

N

F

D

T

S

G

G

V

I

D

V

A

G

I

C

L

L

S

T

V

V

V

T

P

P

Y
|
Y

N

N

K

R

P

P

S

S

Q

Q

E

E

G

G

I

L

Y

Y

Q

Q

H

H

Y

F

K

K

A

A

I

I

S

A

E

E

A

H

T

T

E

D

L

L

P

P

I

Q

V

I

L

L

Y
|
Y

N

N

V

V

P

P

G

S

R
|
R

T

T

G

G

V

C

N

D

M

L

T

L

A

P

E

E

T

T

T

V

L

G

R

R

I

L

A

A

R

K

N

-

F

V

S

K

N

N

V

I

I

I

A

G

I

I

K
|
K

E

E

A

A

S

T

G

G

N

N

I

L

T

T

Q

R

M

V

D

N

D

Q

I

I

I

K

K

E

N

L

K

V

P

S

D

D

N

D

F

F

N

V

V

L

I

L

S

S

G

G

D

D

G

A

I

S

T

A

F

L

P

D

L

F

I

M

T

Q

L

L

G

G

A

G

V

H

G

G

V

V

I
|
I

S

S

V

V

I

T

G

A

N

N

A

V

F

A

P

A

R

R

E

D

F

M

S

A

R

Q

M

M

T

C

R

K

L

L

A

A

L

A

Q

E

G

G

D

H

F

F

A

A

N

E

A

A

L

R

T

V

I

I

H

N

H

Q

R

R

F

L

T

M

E

P

L

L

F

H

D

N

L

K

L

L

F

F

V

V

D

E

G

P

N

N

P

P

A

I

G

P

V

V

K

K

S

W

M

A

L

C

N

K

A

E

M

L

G

G

M

L

I

V

E

A

-

T

N

D

K

T

L

L

R

R

L

L

P

P

L

M

V

T

P

P

T

I

R

T

I

D

T

S

T

G

F

R

E

E

A

T

I

V

R

R

K

A

V

A

L

L

N

K

E

H

L

A

N

G

I

L

active site: T44 (= T48), Y107 (= Y111), Y133 (= Y137), R138 (= R142), K161 (= K166), I203 (= I208)
binding d-lysine: A49 (≠ P53), L51 (= L55), H53 (≠ E57), H56 (≠ K60), N80 (= N84), E84 (≠ A88), Y106 (= Y110)

P0A6L2 4-hydroxy-tetrahydrodipicolinate synthase; HTPA synthase; EC 4.3.3.7 from Escherichia coli (strain K12) (see 7 papers)
39% identity, 96% coverage: 11:295/297 of query aligns to 7:291/292 of P0A6L2

query
sites
P0A6L2

V

V

A

A

L

I

I

V

T

T

P

P

F

M

K

D

E

E

D

K

E

G

S

N

V

V

D

C

Y

R

D

A

A

S

L

L

M

K

R

K

L

L

V

I

D

D

Y

Y

L

H

L

V

Q

A

N

S

N

G

A

T

D

S

F

A

L

I

C

V

V

S

L

V

G

G

T
|
T

A

G

E

E

T

S

P

A

T

T

L

L

T

N

E

H

E

D

E

E

K

H

K

A

T

D

I

V

K

V

K

M

M

M

V

T

I

L

D

D

R

L

V

A

N

D

G

G

R

R

I

I

P

P

I

V

L

I

L

A

G

G

V

T

G

G

G

A

N

N

N

A

T

T

R

A

A

E

I

A

V

I

E

S

T

L

L

T

K

Q

N

R

D

F

D

N

F

D

T

S

G

G

V

I

D

V

A

G

I

C

L

L

S

T

V

V

V

T

P

P

Y

Y

Y
|
Y

N

N

K

R

P

P

S

S

Q

Q

E

E

G

G

I

L

Y

Y

Q

Q

H

H

Y

F

K

K

A

A

I

I

S

A

E

E

A

H

T

T

E

D

L

L

P

P

I

Q

V

I

L

L

Y
|
Y

N

N

V

V

P

P

G

S

R
|
R

T

T

G

G

V

C

N

D

M

L

T

L

A

P

E

E

T

T

T

V

L

G

R

R

I

L

A

A

R

K

N

-

F

V

S

K

N

N

V

I

I

I

A

G

I

I

K
|
K

E

E

A

A

S

T

G

G

N

N

I

L

T

T

Q

R

M

V

D

N

D

Q

I

I

I

K

K

E

N

L

K

V

P

S

D

D

N

D

F

F

N

V

V

L

I

L

S

S

G

G

D

D

G

A

I

S

T

A

F

L

P

D

L

F

I

M

T

Q

L
|
L

G

G

A

G

V

H

G

G

V

V

I
|
I

S

S

V

V

I

T

G

A

N

N

A

V

F

A

P

A

R

R

E

D

F

M

S

A

R

Q

M

M

T

C

R

K

L

L

A

A

L

A

Q

E

G

G

D

H

F

F

A

A

N

E

A

A

L

R

T

V

I

I

H

N

H

Q

R

R

F

L

T

M

E

P

L

L

F

H

D

N

L

K

L

L

F

F

V

V

D

E

G

P

N

N

P

P

A

I

G

P

V

V

K

K

S

W

M

A

L

C

N

K

A

E

M

L

G

G

M

L

I

V

E

A

-

T

N

D

K

T

L

L

R

R

L

L

P

P

L

M

V

T

P

P

T

I

R

T

I

D

T

S

T

G

F

R

E

E

A

T

I

V

R

R

K

A

V

A

L

L

N

K

E

H

L

A

N

G

I

L

T44 (= T48) mutation to S: 8% of wild-type activity. 4-fold decrease in affinity for pyruvate, but nearly no change in that for (S)-ASA.; mutation to V: Reduced kcat by 99.9%.
T45 (= T49) binding pyruvate
Y107 (= Y111) mutation to F: Reduced kcat by 90%.; mutation to W: Reduced activity by 95%. Reduced affinity for both substrates. Exists as a mixture of monomer, dimer and tetramer in solution. Has significantly lower thermal stability than the wild-type enzyme.
Y133 (= Y137) mutation to F: Reduced kcat by 99.7%. Reduced affinity for both substrates.
R138 (= R142) mutation R->A,H: Strongly increased KM for L-aspartate 4-semialdehyde. No effect on KM for pyruvate. Reduced activity by 99.7%.
K161 (= K166) mutation to A: 0.1% of wild-type activity. 3-fold decrease in affinity for pyruvate, and 2-fold decrease in that for (S)-ASA.; mutation to R: 0.35% of wild-type activity. 3-fold decrease in affinity for pyruvate, but nearly no change in that for (S)-ASA.
L197 (= L202) mutation L->Y,D: 1.4 to 2.5% of wild-type activity. Decrease in affinity for pyruvate, but nearly no change in that for (S)-ASA. Exists as a dimer in solution.
I203 (= I208) binding pyruvate

5t25A Kinetic, spectral and structural characterization of the slow binding inhibitor acetopyruvate with dihydrodipicolinate synthase from escherichia coli.
39% identity, 96% coverage: 11:295/297 of query aligns to 8:292/293 of 5t25A

query
sites
5t25A

V

V

A

A

L

I

I

V

T

T

P

P

F

M

K

D

E

E

D

K

E

G

S

N

V

V

D

C

Y

R

D

A

A

S

L

L

M

K

R

K

L

L

V

I

D

D

Y

Y

L

H

L

V

Q

A

N

S

N

G

A

T

D

S

F

A

L

I

C

V

V

S

L

V

G

G

T
|
T

T

T

A

G

E

E

T

S

P
x
A

T

T

L
|
L

T

N

E
x
H

E

D

E

E

K

H

K

A

T

D

I

V

K

V

K

M

M

M

V

T

I

L

D

D

R

L

V

A

N

D

G

G

R

R

I

I

P

P

I

V

L

I

L

A

G

G

V

T

G

G

G

A

N
|
N

N

A

T

T

R

A

A
x
E

I

A

V

I

E

S

T

L

L

T

K

Q

N

R

D

F

D

N

F

D

T

S

G

G

V

I

D

V

A

G

I

C

L

L

S

T

V

V

V

T

P

P

Y
|
Y

N

N

K

R

P

P

S

S

Q

Q

E

E

G

G

I

L

Y

Y

Q

Q

H

H

Y

F

K

K

A

A

I

I

S

A

E

E

A

H

T

T

E

D

L

L

P

P

I

Q

V

I

L

L

Y
|
Y

N

N

V

V

P

P

G

S

R
|
R

T

T

G

G

V

C

N

D

M

L

T

L

A

P

E

E

T

T

T

V

L

G

R

R

I

L

A

A

R

K

N

-

F

V

S

K

N

N

V

I

I

I

A

G

I

I

K
|
K

E

E

A

A

S

T

G

G

N

N

I

L

T

T

Q

R

M

V

D

N

D

Q

I

I

I

K

K

E

N

L

K

V

P

S

D

D

N

D

F

F

N

V

V

L

I

L

S

S

G

G

D

D

G

A

I

S

T

A

F

L

P

D

L

F

I

M

T

Q

L

L

G

G

A

G

V

H

G

G

V

V

I
|
I

S

S

V

V

I

T

G

A

N

N

A

V

F

A

P

A

R

R

E

D

F

M

S

A

R

Q

M

M

T

C

R

K

L

L

A

A

L

A

Q

E

G

G

D

H

F

F

A

A

N

E

A

A

L

R

T

V

I

I

H

N

H

Q

R

R

F

L

T

M

E

P

L

L

F

H

D

N

L

K

L

L

F

F

V

V

D

E

G

P

N

N

P

P

A

I

G

P

V

V

K

K

S

W

M

A

L

C

N

K

A

E

M

L

G

G

M

L

I

V

E

A

-

T

N

D

K

T

L

L

R

R

L

L

P

P

L

M

V

T

P

P

T

I

R

T

I

D

T

S

T

G

F

R

E

E

A

T

I

V

R

R

K

A

V

A

L

L

N

K

E

H

L

A

N

G

I

L

active site: T45 (= T48), Y108 (= Y111), Y134 (= Y137), R139 (= R142), K162 (= K166), I204 (= I208)
binding lysine: A50 (≠ P53), L52 (= L55), H54 (≠ E57), N81 (= N84), E85 (≠ A88), Y107 (= Y110)

3i7sA Dihydrodipicolinate synthase mutant - k161a - with the substrate pyruvate bound in the active site. (see paper)
39% identity, 96% coverage: 11:295/297 of query aligns to 7:291/292 of 3i7sA

query
sites
3i7sA

V

V

A
|
A

L

I

I

V

T

T

P

P

F

M

K

D

E

E

D

K

E

G

S

N

V

V

D

C

Y

R

D

A

A

S

L

L

M

K

R

K

L

L

V

I

D

D

Y

Y

L

H

L

V

Q

A

N

S

N

G

A

T

D

S

F

A

L

I

C

V

V

S

L

V

G

G

T
|
T

A

G

E

E

T

S

P

A

T

T

L

L

T

N

E

H

E

D

E

E

K

H

K

A

T

D

I

V

K

V

K

M

M

M

V

T

I

L

D

D

R

L

V

A

N

D

G

G

R

R

I

I

P

P

I

V

L

I

L

A

G

G

V

T

G

G

G

A

N

N

N

A

T

T

R

A

A

E

I

A

V

I

E

S

T

L

L

T

K

Q

N

R

D

F

D

N

F

D

T

S

G

G

V

I

D

V

A

G

I

C

L

L

S

T

V

V

V

T

P

P

Y

Y

Y
|
Y

N

N

K

R

P

P

S

S

Q

Q

E

E

G

G

I

L

Y

Y

Q

Q

H

H

Y

F

K

K

A

A

I

I

S

A

E

E

A

H

T

T

E

D

L

L

P

P

I

Q

V

I

L

L

Y
|
Y

N

N

V

V

P

P

G

S

R
|
R

T

T

G

G

V

C

N

D

M

L

T

L

A

P

E

E

T

T

T

V

L

G

R

R

I

L

A

A

R

K

N

-

F

V

S

K

N

N

V

I

I

I

A

G

I

I

K
x
R

E

E

A

A

S

T

G

G

N

N

I

L

T

T

Q

R

M

V

D

N

D

Q

I

I

I

K

K

E

N

L

K

V

P

S

D

D

N

D

F

F

N

V

V

L

I

L

S

S

G

G

D

D

G

A

I

S

T

A

F

L

P

D

L

F

I

M

T

Q

L

L

G

G

A

G

V

H

G

G

V

V

I
|
I

S

S

V

V

I

T

G

A

N

N

A

V

F

A

P

A

R

R

E

D

F

M

S

A

R

Q

M

M

T

C

R

K

L

L

A

A

L

A

Q

E

G

G

D

H

F

F

A

A

N

E

A

A

L

R

T

V

I

I

H

N

H

Q

R

R

F

L

T

M

E

P

L

L

F

H

D

N

L

K

L

L

F

F

V

V

D

E

G

P

N

N

P

P

A

I

G

P

V

V

K

K

S

W

M

A

L

C

N

K

A

E

M

L

G

G

M

L

I

V

E

A

-

T

N

D

K

T

L

L

R

R

L

L

P

P

L

M

V

T

P

P

T

I

R

T

I

D

T

S

T

G

F

R

E

E

A

T

I

V

R

R

K

A

V

A

L

L

N

K

E

H

L

A

N

G

I

L

active site: T44 (= T48), Y107 (= Y111), Y133 (= Y137), R138 (= R142), R161 (≠ K166), I203 (= I208)
binding pyruvic acid: A8 (= A12), T44 (= T48), T45 (= T49), Y133 (= Y137)

7kg2A Dihydrodipicolinate synthase (dhdps) from c.Jejuni, h59k mutant with pyruvate bound in the active site and l-histidine bound at the allosteric site
38% identity, 97% coverage: 8:296/297 of query aligns to 6:294/296 of 7kg2A

query
sites
7kg2A

G

G

M

A

G

M

V

T

A

A

L

L

I

I

T

T

P

P

F

F

K

K

E

N

D

G

E

K

S

-

V

V

D

D

Y

E

D

Q

A

S

L

Y

M

A

R

R

L

L

V

I

D

K

Y

R

L

Q

L

I

Q

E

N

N

N

G

A

I

D

D

F

A

L

V

C

V

V

P

L

V

G

G

T

T

A

G

E

E

T
x
S

P
x
A

T

T

L
|
L

T

T

E

H

E

E

K
|
K

K

R

T

T

I

C

K

I

K

E

M

I

V

A

I

V

D

E

R

T

V

C

N

K

G

G

-

T

R

K

I

V

P

K

I

V

L

L

L

A

G

G

V

A

G

G

G

S

N
|
N

N

A

T

T

R

H

A
x
E

I

A

V

V

E

G

T

L

L

A

K

K

N

F

D

A

D

K

F

E

T

H

G

G

V

A

D

D

A

G

I

I

L

L

S

S

V

V

V

A

P

P

Y
|
Y

Y

Y

N

N

K

K

P

P

S

T

Q

Q

E

Q

G

G

I

L

Y

Y

Q

E

H

H

Y

Y

K

K

A

A

I

I

S

A

E

Q

A

S

T

V

E

D

L

I

P

P

I

V

V

L

L

L

Y

Y

N

N

V

V

P

P

G

G

R

R

T

T

G

G

V

C

N

E

M

I

T

S

A

T

E

D

T

T

T

I

L

I

R

K

I

L

A

F

R

R

N

D

F

C

S

E

N

N

V

I

I

Y

A

G

I

V

K

K

E

E

A

A

S

S

G

G

N

N

I

I

T

D

Q

K

M

C

D

V

D

D

I

L

K

A

N

H

K

E

P

P

D

-

N

R

F

M

N

M

V

L

I

I

S

S

G

G

D

E

D

D

G

A

I

I

T

N

F

Y

P

P

L

I

I

L

T

S

L

N

G

G

A

G

V

K

G

G

V

V

I

I

S

S

V

V

I

T

G

S

N

N

A

L

F

L

P

P

R

D

E

M

F

I

S

S

R

A

M

L

T

T

R

H

L

F

A

A

L

L

Q

D

G

E

D

N

F

Y

A

K

N

E

A

A

L

K

T

K

I

I

H

N

H

D

R

E

F

L

T

Y

E

N

L

I

F

N

D

K

L

I

L

L

F

F

V

C

D

E

G

S

N

N

P

P

A

I

G

P

V

I

K

K

S

T

M

A

L

M

N

Y

A

L

M

A

G

G

M

L

I

I

E

E

N

S

-

L

K

E

L

F

R

R

L

L

P

P

L

L

V

C

P

S

T

P

R

S

I

K

T

E

T

N

F

F

E

A

A

K

I

I

R

E

K

E

V

V

L

M

N

K

E

K

L

Y

N

K

I

I

K

K

binding histidine: S49 (≠ T52), A50 (≠ P53), L52 (= L55), K57 (= K60), N82 (= N84), E86 (≠ A88), Y108 (= Y110)

5ktlA Dihydrodipicolinate synthase from the industrial and evolutionarily important cyanobacteria anabaena variabilis. (see paper)
39% identity, 96% coverage: 12:295/297 of query aligns to 11:294/295 of 5ktlA

query
sites
5ktlA

A

A

L

M

I

I

T

T

P

P

F

F

K

K

E

A

D

D

E

G

S

S

V

V

D

N

Y

Y

D

A

A

V

L

A

M

A

R

E

L

L

V

A

D

A

Y

H

L

L

L

V

Q

D

N

N

N

G

A

T

D

D

F

T

L

L

C

V

V

V

L

C

G

G

T
|
T

T

T

A

G

E

E

T
x
S

P

P

T

T

L
|
L

T

S

E

W

E

D

E

E

K
x
E

K

Y

T

N

I

L

K

F

K

V

M
x
E

V

V

I

L

D
x
Q

R

T

V

V

N

A

G

G

R

K

I

A

P

K

I

V

L

I

L

A

G

G

V

C

G

G

G

S

N

N

N

S

T

T

R

K

A

E

I

A

V

I

E

A

T

A

L

T

K

Q

N

K

D

A

F

K

T

I

G

G

V

V

D

H

A

G

I

T

L

L

S

Q

V

V

P

P

Y

Y

Y
|
Y

N

N

K

K

P

P

S

P

Q

Q

E

A

G

G

I

L

Y

Y

Q

Q

H

H

Y

F

K

Q

A

A

I

I

S

A

E

Q

A

A

T

C

-

P

E

D

L

L

P

P

I

L

V

L

L

L

Y
|
Y

N

N

V

V

P

P

G

G

R
|
R

T

T

G

G

V

Q

N

N

M

L

T

S

A

P

E

E

T

T

T

V

L

V

R

R

I

L

A

A

R

E

N

-

F

I

S

D

N

N

V

I

I

I

A

G

I

V

K
|
K

E

E

A

A

S

S

G

G

N

N

I

L

T

D

Q

Q

M

A

D

G

D

E

I

I

I

R

K

R

N

S

K

T

P

P

D

K

N

E

F

F

N

Q

V

I

I

Y

S

A

G

G

D

D

G

S

I

L

T

T

F

L

P

P

L

L

I

L

T

A

L

I

G

G

A

A

V

K

G

G

V

V

I
x
V

S

S

V

V

I

A

G

S

N

H

A

L

F

V

P

G

R

N

E

Q

F

L

S

Q

R

Q

M

M

T

I

R

Q

L

A

A

F

L

N

Q

S

G

G

D

Q

F

V

A

T

N

V

A

A

L

S

T

D

I

I

H

H

H

L

R

R

F

L

T

L

E

P

L

L

F

F

D

K

L

A

L

L

F

F

V

I

D

T

G

T

N

N

P

P

A

I

G

P

V

I

K

K

S

Q

M

A

L

L

N

K

A

L

M

Q

G

G

M

W

I

E

E

V

N

G

K

S

L

T

R

R

L

P

P

P

L

L

V

S

P

D

T

A

R

D

I

A

T

E

T

V

F

S

E

Q

A

K

I

L

R

E

K

A

V

V

L

M

N

K

E

H

L

L

N

N

I

L

active site: T47 (= T48), Y110 (= Y111), Y137 (= Y137), R142 (= R142), K165 (= K166), V207 (≠ I208)
binding manganese (ii) ion: S51 (≠ T52), L54 (= L55), E59 (≠ K60), E65 (≠ M66), Q68 (≠ D69)

4m19A Dihydrodipicolinate synthase from c. Jejuni with pyruvate bound to the active site and lysine bound to allosteric site (see paper)
38% identity, 97% coverage: 8:296/297 of query aligns to 6:294/296 of 4m19A

query
sites
4m19A

G

G

M

A

G

M

V

T

A

A

L

L

I

I

T

T

P

P

F

F

K

K

E

N

D

G

E

K

S

-

V

V

D

D

Y

E

D

Q

A

S

L

Y

M

A

R

R

L

L

V

I

D

K

Y

R

L

Q

L

I

Q

E

N

N

N

G

A

I

D

D

F

A

L

V

C

V

V

P

L

V

G

G

T
|
T

T

T

A

G

E

E

T
x
S

P
x
A

T

T

L
|
L

T

T

E
x
H

E

E

K
x
H

K

R

T

T

I

C

K

I

K

E

M

I

V

A

I

V

D

E

R

T

V

C

N

K

G

G

-

T

R

K

I

V

P

K

I

V

L

L

L

A

G

G

V

A

G

G

G

S

N
|
N

N

A

T

T

R

H

A
x
E

I

A

V

V

E

G

T

L

L

A

K

K

N

F

D

A

D

K

F

E

T

H

G

G

V

A

D

D

A

G

I

I

L

L

S

S

V

V

V

A

P

P

Y
|
Y

N

N

K

K

P

P

S

T

Q

Q

E

Q

G

G

I

L

Y

Y

Q

E

H

H

Y

Y

K

K

A

A

I

I

S

A

E

Q

A

S

T

V

E

D

L

I

P

P

I

V

V

L

L

L

Y
|
Y

N

N

V

V

P

P

G

G

R
|
R

T

T

G

G

V

C

N

E

M

I

T

S

A

T

E

D

T

T

T

I

L

I

R

K

I

L

A

F

R

R

N

D

F

C

S

E

N

N

V

I

I

Y

A

G

I

V

K
|
K

E

E

A

A

S

S

G

G