SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 350804 FitnessBrowser__Btheta:350804 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

2qlxA Crystal structure of rhamnose mutarotase rhau of rhizobium leguminosarum in complex with l-rhamnose (see paper)
28% identity, 76% coverage: 29:127/130 of query aligns to 15:107/108 of 2qlxA

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2qlxA

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binding beta-L-rhamnopyranose: Y22 (= Y36), H26 (= H40), Y45 (≠ M61), W80 (= W98), W81 (≠ E99)

Sites not aligning to the query:

binding beta-L-rhamnopyranose: 12

2qlwB Crystal structure of rhamnose mutarotase rhau of rhizobium leguminosarum (see paper)
28% identity, 76% coverage: 29:127/130 of query aligns to 15:107/108 of 2qlwB

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2qlwB

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binding magnesium ion: A85 (≠ S103), M88 (≠ Q106), T90 (≠ A108)

Query Sequence

>350804 FitnessBrowser__Btheta:350804
METPQTGYQVKTYNVPVKRYCQTLDLRDSPELIAEYRKRHSQAEVWPEILAGIREVGILE
MEIYILGTRLFMIVETPVDFDWDTAMARLNTLPRQQEWEEYMSILQQAAPGMSSAEKWIP
MERMFHLYNT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory