SitesBLAST
Comparing 350819 FitnessBrowser__Btheta:350819 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
48% identity, 69% coverage: 9:326/463 of query aligns to 17:344/378 of P69874
- C26 (≠ F18) mutation to A: Lower ATPase activity and transport efficiency.
- F27 (= F19) mutation to L: Lower ATPase activity and transport efficiency.
- F45 (= F37) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (= C46) mutation to T: Loss of ATPase activity and transport.
- L60 (= L52) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (≠ I68) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (= V127) mutation to M: Loss of ATPase activity and transport.
- D172 (= D164) mutation to N: Loss of ATPase activity and transport.
- C276 (= C265) mutation to A: Lower ATPase activity and transport efficiency.
- E297 (= E283) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.
8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
48% identity, 69% coverage: 9:326/463 of query aligns to 2:329/358 of 8y5iA
2d62A Crystal structure of multiple sugar binding transport atp-binding protein
46% identity, 60% coverage: 10:286/463 of query aligns to 7:298/375 of 2d62A
1g291 Malk (see paper)
46% identity, 60% coverage: 10:286/463 of query aligns to 4:295/372 of 1g291
- binding magnesium ion: D69 (≠ Q75), E71 (vs. gap), K72 (vs. gap), K79 (≠ H79), D80 (≠ K80), E292 (= E283), D293 (= D284)
- binding pyrophosphate 2-: S38 (= S44), G39 (= G45), C40 (= C46), G41 (= G47), K42 (= K48), T43 (= T49), T44 (= T50)
Sites not aligning to the query:
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
46% identity, 51% coverage: 10:245/463 of query aligns to 3:238/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F19), S37 (= S44), G38 (= G45), C39 (= C46), G40 (= G47), K41 (= K48), S42 (≠ T49), T43 (= T50), Q81 (= Q88), R128 (= R135), A132 (≠ S139), S134 (= S141), G136 (= G143), Q137 (= Q144), E158 (= E165), H191 (= H198)
- binding magnesium ion: S42 (≠ T49), Q81 (= Q88)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
46% identity, 51% coverage: 10:245/463 of query aligns to 3:238/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ F19), G38 (= G45), C39 (= C46), G40 (= G47), K41 (= K48), S42 (≠ T49), T43 (= T50), R128 (= R135), S134 (= S141), Q137 (= Q144)
- binding beryllium trifluoride ion: S37 (= S44), G38 (= G45), K41 (= K48), Q81 (= Q88), S134 (= S141), G136 (= G143), H191 (= H198)
- binding magnesium ion: S42 (≠ T49), Q81 (= Q88)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
46% identity, 51% coverage: 10:245/463 of query aligns to 3:238/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ F19), V17 (≠ A24), G38 (= G45), C39 (= C46), G40 (= G47), K41 (= K48), S42 (≠ T49), T43 (= T50), R128 (= R135), A132 (≠ S139), S134 (= S141), Q137 (= Q144)
- binding tetrafluoroaluminate ion: S37 (= S44), G38 (= G45), K41 (= K48), Q81 (= Q88), S134 (= S141), G135 (= G142), G136 (= G143), E158 (= E165), H191 (= H198)
- binding magnesium ion: S42 (≠ T49), Q81 (= Q88)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
46% identity, 51% coverage: 10:245/463 of query aligns to 3:238/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ F19), V17 (≠ A24), G38 (= G45), C39 (= C46), G40 (= G47), K41 (= K48), S42 (≠ T49), T43 (= T50), R128 (= R135), A132 (≠ S139), S134 (= S141), Q137 (= Q144)
- binding magnesium ion: S42 (≠ T49), Q81 (= Q88)
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
46% identity, 51% coverage: 10:245/463 of query aligns to 1:236/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ F19), S35 (= S44), G36 (= G45), C37 (= C46), G38 (= G47), K39 (= K48), S40 (≠ T49), T41 (= T50), R126 (= R135), A130 (≠ S139), S132 (= S141), G134 (= G143), Q135 (= Q144)
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
46% identity, 51% coverage: 10:245/463 of query aligns to 4:239/371 of P68187
- A85 (= A91) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ P112) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (= V120) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ A123) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ K125) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ T130) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G143) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D164) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (≠ S234) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
46% identity, 51% coverage: 10:245/463 of query aligns to 3:238/374 of 2awnB
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
45% identity, 51% coverage: 10:245/463 of query aligns to 4:239/369 of P19566
- L86 (= L92) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P166) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D171) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
49% identity, 50% coverage: 10:241/463 of query aligns to 4:236/393 of P9WQI3
- H193 (= H198) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.
8hprC Lpqy-sugabc in state 4 (see paper)
46% identity, 50% coverage: 10:241/463 of query aligns to 3:235/363 of 8hprC
- binding adenosine-5'-triphosphate: Y12 (≠ F19), S38 (= S44), G39 (= G45), G41 (= G47), K42 (= K48), S43 (≠ T49), Q82 (= Q88), Q133 (≠ S139), G136 (= G142), G137 (= G143), Q138 (= Q144), H192 (= H198)
- binding magnesium ion: S43 (≠ T49), Q82 (= Q88)
8hprD Lpqy-sugabc in state 4 (see paper)
46% identity, 50% coverage: 10:241/463 of query aligns to 3:235/362 of 8hprD
- binding adenosine-5'-triphosphate: Y12 (≠ F19), S38 (= S44), C40 (= C46), G41 (= G47), K42 (= K48), S43 (≠ T49), T44 (= T50), Q82 (= Q88), R129 (= R135), Q133 (≠ S139), S135 (= S141), G136 (= G142), G137 (= G143), Q159 (≠ E165), H192 (= H198)
- binding magnesium ion: S43 (≠ T49), Q82 (= Q88)
8hplC Lpqy-sugabc in state 1 (see paper)
46% identity, 50% coverage: 10:241/463 of query aligns to 3:233/384 of 8hplC
1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
48% identity, 51% coverage: 10:246/463 of query aligns to 7:237/353 of 1vciA
2awnC Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
44% identity, 48% coverage: 23:245/463 of query aligns to 9:208/344 of 2awnC