SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 350849 FitnessBrowser__Btheta:350849 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0AAH0 Phosphate import ATP-binding protein PstB; ABC phosphate transporter; Phosphate-transporting ATPase; EC 7.3.2.1 from Escherichia coli (strain K12) (see paper)
60% identity, 98% coverage: 5:252/252 of query aligns to 10:257/257 of P0AAH0

query
sites
P0AAH0

K

K

I

I

D

Q

T

V

R

R

D

N

V

L

N

N

F

F

W

Y

Y

Y

G

G

D

K

F

F

H

H

A

A

L

L

K

K

G

N

I

I

S

N

M

L

Q

D

I

I

E

A

E

K

K

N

S

Q

V

V

V

T

A

A

F

F

I

I

G

G

P

P

S

S

G

G

C

C

G
|
G

K
|
K

S

S

T

T

F

L

L

L

R

R

L

T

F

F

N

N

R

K

M

M

N

F

D

E

L

L

I

Y

P

P

A

E

T

Q

R

R

L

A

E

E

G

G

E

E

I

I

R

L

I

L

D

D

G

G

H

D

N

N

I

I

Y

L

A

T

K

N

G

S

V

Q

E

D

V

I

D

A

E

L

L

L

R

R

K

A

N

K

V

V

G

G

M

M

V

V

F

F

Q

Q

R

K

P

P

N

T

P

P

F

F

P

P

K

M

S

S

I

I

F

Y

E

D

N

N

V

I

A

A

Y

F

G

G

L

V

R

R

V

L

N

F

G

E

V

K

K

L

D

S

N

R

A

A

F

D

I

M

R

D

Q

E

R

R

V

V

E

Q

E

W

T

A

L

L

K

T

G

K

A

A

L

L

W

W

D

N

E

E

V

T

K

K

D

D

K

K

L

L

K

H

E

Q

S

S

A

G

Y

Y

A

S

L

L

S

S

G

G

Q

Q

R

R

L

L

C

C

I

I

A

A

R

R

A

G

M

I

A

A

V

I

S

R

P

P

S

E

V

V

L

L

M

L

D

D

E

E

P

P

A

C

S

S

A

A

L

L

D

D

P

P

I

I

S

S

T

T

A

G

K

R

V

I

E

E

L

L

I

I

H

T

E

E

L

L

K

K

K

Q

D

D

Y

Y

T

T

I

V

V

V

I

I

V

V

T

T

H

H

N

N

M

M

Q

Q

A

A

R

R

V

C

S

S

D

D

K

H

T

T

A

A

F

F

F

M

Y

Y

L

L

G

G

E

E

M

L

V

I

E

E

Y

F

D

S

D

N

T

T

K

D

I

L

F

F

T

T

N

K

P

P

E

A

K

K

E

K

A

Q

T

T

Q

E

N

D

Y

Y

I

I

T

T

G

G

R

R

F

Y

G

G

G48 (= G43) mutation to I: Loss of phosphate transport.
K49 (= K44) mutation to Q: Loss of phosphate transport.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

3c4jA Abc protein artp in complex with atp-gamma-s
40% identity, 98% coverage: 6:251/252 of query aligns to 4:242/242 of 3c4jA

query
sites
3c4jA

I

I

D

D

T

V

R

H

D

Q

V

L

N

K

F

K

W

S

Y
x
F

G

G

D

S

F

L

H

E

A
x
V

L

L

K

K

G

G

I

I

S

N

M

V

Q

H

I

I

E

R

E

E

K

G

S

E

V

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

L

C

F

L

N

N

R

L

M

L

N

E

D

D

L

F

I

D

P

-

A

-

T

-

R

-

L

-

E

E

G

G

E

E

I

I

R

I

I

I

D

D

G

G

H

I

N

N

I

L

Y

K

A

A

K

K

G

D

V

T

E

N

V

L

D

N

E

K

L

V

R

R

K

E

N

E

V

V

G

G

M

M

V

V

F

F

Q

Q

R

R

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

F

L

E

N

N

N

V

I

A

T

Y

L

G

A

L

-

R

-

V

-

N

-

G

P

V

M

K

K

D

V

N

R

A

K

F

W

I

P

R

R

Q

E

R

K

V

A

E

E

E

A

T

-

L

-

K

K

G

A

A

M

A

E

L

L

W

L

D

D

E

K

V

V

K

G

D

L

K

K

L

D

K

K

E

A

S

H

A

A

Y

Y

-

P

-

D

A

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

M

L

A

A

V

M

S

E

P

P

S

K

V

I

L

M

L

L

M

F

D

D

E

E

P

P

A

T

S

S

A

A

L

L

D

D

P

P

I

E

S

M

T

V

A

G

K

E

V

V

E

L

E

S

L

V

I

M

H

K

E

Q

L

L

K

A

K

N

D

E

-

G

Y

M

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

D

D

K

R

T

V

A

L

F

F

F

M

Y

D

L

G

G

G

E

Y

M

I

V

I

E

E

Y

E

D

G

D

K

T

P

K

E

K

D

I

L

F

F

T

D

N

R

P

P

E

Q

K

H

E

E

A

R

T

T

Q

K

N

A

Y

F

I

L

T

S

G

K

R

V

F

F

binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y15), V18 (≠ A20), S38 (= S40), G39 (= G41), S40 (≠ C42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)

3c41J Abc protein artp in complex with amp-pnp/mg2+
40% identity, 98% coverage: 6:251/252 of query aligns to 4:242/242 of 3c41J

query
sites
3c41J

I

I

D

D

T

V

R

H

D

Q

V

L

N

K

F

K

W

S

Y
x
F

G

G

D

S

F

L

H

E

A
x
V

L

L

K

K

G

G

I

I

S

N

M

V

Q

H

I

I

E

R

E

E

K

G

S

E

V

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

L

C

F

L

N

N

R

L

M

L

N

E

D

D

L

F

I

D

P

-

A

-

T

-

R

-

L

-

E

E

G

G

E

E

I

I

R

I

I

I

D

D

G

G

H

I

N

N

I

L

Y

K

A

A

K

K

G

D

V

T

E

N

V

L

D

N

E

K

L

V

R

R

K

E

N

E

V

V

G

G

M

M

V

V

F

F

Q

Q

R

R

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

F

L

E

N

N

N

V

I

A

T

Y

L

G

A

L

-

R

-

V

-

N

-

G

P

V

M

K

K

D

V

N

R

A

K

F

W

I

P

R

R

Q

E

R

K

V

A

E

E

E

A

T

-

L

-

K

K

G

A

A

M

A

E

L

L

W

L

D

D

E

K

V

V

K

G

D

L

K

K

L

D

K

K

E

A

S

H

A

A

Y

Y

-

P

-

D

A

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

M

L

A

A

V

M

S

E

P

P

S

K

V

I

L

M

L

L

M

F

D
|
D

E

E

P

P

A

T

S

S

A

A

L

L

D

D

P

P

I

E

S

M

T

V

A

G

K

E

V

V

E

L

E

S

L

V

I

M

H

K

E

Q

L

L

K

A

K

N

D

E

-

G

Y

M

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

D

D

K

R

T

V

A

L

F

F

F

M

Y

D

L

G

G

G

E

Y

M

I

V

I

E

E

Y

E

D

G

D

K

T

P

K

E

K

D

I

L

F

F

T

D

N

R

P

P

E

Q

K

H

E

E

A

R

T

T

Q

K

N

A

Y

F

I

L

T

S

G

K

R

V

F

F

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y15), V18 (≠ A20), S38 (= S40), G39 (= G41), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)
binding magnesium ion: S43 (= S45), D163 (= D173)

2olkA Abc protein artp in complex with adp-beta-s
40% identity, 98% coverage: 6:251/252 of query aligns to 4:242/242 of 2olkA

query
sites
2olkA

I

I

D

D

T

V

R

H

D

Q

V

L

N

K

F

K

W

S

Y
x
F

G

G

D

S

F

L

H

E

A
x
V

L

L

K

K

G

G

I

I

S

N

M

V

Q

H

I

I

E

R

E

E

K

G

S

E

V

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

L

C

F

L

N

N

R

L

M

L

N

E

D

D

L

F

I

D

P

-

A

-

T

-

R

-

L

-

E

E

G

G

E

E

I

I

R

I

I

I

D

D

G

G

H

I

N

N

I

L

Y

K

A

A

K

K

G

D

V

T

E

N

V

L

D

N

E

K

L

V

R

R

K

E

N

E

V

V

G

G

M

M

V

V

F

F

Q

Q

R

R

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

F

L

E

N

N

N

V

I

A

T

Y

L

G

A

L

-

R

-

V

-

N

-

G

P

V

M

K

K

D

V

N

R

A

K

F

W

I

P

R

R

Q

E

R

K

V

A

E

E

E

A

T

-

L

-

K

K

G

A

A

M

A

E

L

L

W

L

D

D

E

K

V

V

K

G

D

L

K

K

L

D

K

K

E

A

S

H

A

A

Y

Y

-

P

-

D

A

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

M

L

A

A

V

M

S

E

P

P

S

K

V

I

L

M

L

L

M

F

D

D

E

E

P

P

A

T

S

S

A

A

L

L

D

D

P

P

I

E

S

M

T

V

A

G

K

E

V

V

E

L

E

S

L

V

I

M

H

K

E

Q

L

L

K

A

K

N

D

E

-

G

Y

M

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

D

D

K

R

T

V

A

L

F

F

F

M

Y

D

L

G

G

G

E

Y

M

I

V

I

E

E

Y

E

D

G

D

K

T

P

K

E

K

D

I

L

F

F

T

D

N

R

P

P

E

Q

K

H

E

E

A

R

T

T

Q

K

N

A

Y

F

I

L

T

S

G

K

R

V

F

F

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y15), V18 (≠ A20), S38 (= S40), G39 (= G41), S40 (≠ C42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)

2oljA Abc protein artp in complex with adp/mg2+
40% identity, 98% coverage: 6:251/252 of query aligns to 4:242/242 of 2oljA

query
sites
2oljA

I

I

D

D

T

V

R

H

D

Q

V

L

N

K

F

K

W

S

Y
x
F

G

G

D

S

F

L

H

E

A
x
V

L

L

K

K

G

G

I

I

S

N

M

V

Q

H

I

I

E

R

E

E

K

G

S

E

V

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

L

C

F

L

N

N

R

L

M

L

N

E

D

D

L

F

I

D

P

-

A

-

T

-

R

-

L

-

E

E

G

G

E

E

I

I

R

I

I

I

D

D

G

G

H

I

N

N

I

L

Y

K

A

A

K

K

G

D

V

T

E

N

V

L

D

N

E

K

L

V

R

R

K

E

N

E

V

V

G

G

M

M

V

V

F

F

Q

Q

R

R

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

F

L

E

N

N

N

V

I

A

T

Y

L

G

A

L

-

R

-

V

-

N

-

G

P

V

M

K

K

D

V

N

R

A

K

F

W

I

P

R

R

Q

E

R

K

V

A

E

E

E

A

T

-

L

-

K

K

G

A

A

M

A

E

L

L

W

L

D

D

E

K

V

V

K

G

D

L

K

K

L

D

K

K

E

A

S

H

A

A

Y

Y

-

P

-

D

A

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

M

L

A

A

V

M

S

E

P

P

S

K

V

I

L

M

L

L

M

F

D

D

E

E

P

P

A

T

S

S

A

A

L

L

D

D

P

P

I

E

S

M

T

V

A

G

K

E

V

V

E

L

E

S

L

V

I

M

H

K

E

Q

L

L

K

A

K

N

D

E

-

G

Y

M

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

D

D

K

R

T

V

A

L

F

F

F

M

Y

D

L

G

G

G

E

Y

M

I

V

I

E

E

Y

E

D

G

D

K

T

P

K

E

K

D

I

L

F

F

T

D

N

R

P

P

E

Q

K

H

E

E

A

R

T

T

Q

K

N

A

Y

F

I

L

T

S

G

K

R

V

F

F

binding adenosine-5'-diphosphate: F13 (≠ Y15), V18 (≠ A20), S38 (= S40), G39 (= G41), S40 (≠ C42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
38% identity, 96% coverage: 6:247/252 of query aligns to 3:236/241 of 4u00A

query
sites
4u00A

I

I

D

R

T

I

R

R

D

N

V

L

N

H

F

K

W

W

Y
x
F

G

G

D

P

F

L

H

H

A
x
V

L

L

K

K

G

G

I

I

S

H

M

L

Q

E

I

V

E

A

E

P

K

G

S

E

V

K

V

L

A

V

F

I

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

L

T

F

I

N

N

R

R

M

L

N

E

D

D

L

F

I

-

P

-

A

-

T

-

R

-

L

Q

E

E

G

G

E

E

I

V

R

V

I

V

D

D

G

G

H

L

N

S

I

V

Y

K

A

D

K

D

G

R

V

A

E

-

V

L

D

R

E

E

L

I

R

R

K

R

N

E

V

V

G

G

M

M

V

V

F

F

Q

Q

R

Q

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

F

L

E

E

N

N

V

V

A

T

Y

L

G

A

-

P

L

M

R

R

V

V

N

R

G

-

V

-

K

-

D

-

N

-

A

R

F

W

I

P

R

R

Q

E

R

K

V

A

E

E

E

K

T

-

L

-

K

K

G

A

A

L

A

E

L

L

W

L

D

E

E

R

V

V

K

G

-

I

-

L

D

D

K

Q

L

A

K

R

E

K

S

Y

A

P

Y

A

A

Q

L

L

S

S

G

G

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

M

L

A

A

V

M

S

E

P

P

S

K

V

I

L

M

L

L

M

F

D

D

E

E

P

P

A

T

S

S

A

A

L

L

D

D

P

P

I

E

S

M

T

V

A

G

K

E

V

V

E

L

E

D

L

V

I

M

H

R

E

D

L

L

K

A

K

Q

-

G

D

G

Y

M

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

A

D

D

K

R

T

V

A

V

F

F

F

M

Y

D

L

G

G

G

E

Q

M

I

V

V

E

E

Y

E

D

G

D

R

T

P

K

E

I

I

F

F

T

T

N

R

P

P

E

K

K

E

E

E

A

R

T

T

Q

R

N

S

Y

F

I

L

binding adenosine-5'-diphosphate: F12 (≠ Y15), V17 (≠ A20), S37 (= S40), G38 (= G41), S39 (≠ C42), G40 (= G43), K41 (= K44), S42 (= S45), T43 (= T46)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
37% identity, 96% coverage: 6:248/252 of query aligns to 2:237/240 of 4ymuJ

query
sites
4ymuJ

I

I

D

F

T

V

R

N

D

D

V

V

N

Y

F

K

W

N

Y
x
F

G

G

D

S

F

L

H

E

A
x
V

L

L

K

K

G

G

I

V

S

T

M

L

Q

K

I

V

E

N

E

K

K

G

S

E

V

V

A

V

F

I

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

R

L

C

F

I

N

N

R

L

M

L

N

E

D

E

L

-

I

-

P

P

A

T

T

-

R

-

L

-

E

K

G

G

E

E

I

V

R

F

I

I

D

D

G

G

H

V

N

K

I

I

Y

N

A

N

K

G

G

K

V

V

E

N

V

I

D

N

E

K

L

V

R

R

K

Q

N

K

V

V

G

G

M

M

V

V

F

F

Q

Q

R

H

P

F

N

N

P

L

F

F

P

P

K

H

-

L

S

T

I

A

F

I

E

E

N

N

V

I

A

T

Y

L

G

A

L

-

R

-

V

-

N

-

G

P

V

V

K

K

D

V

N

K

A

K

F

M

I

N

R

K

Q

K

R

E

V

A

E

E

T

L

L

A

K

V

G

D

A

L

L

A

W

K

D

V

E

G

V

L

K

L

D

D

K

K

L

K

K

D

E

Q

S

Y

A

P

Y

I

A

K

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

L

C

A

I

I

A

A

R

R

A

A

M

L

A

A

V

M

S

Q

P

P

S

E

V

V

L

M

L

L

M

F

D

D

E
|
E

P

P

A

T

S

S

A

A

L

L

D

D

P

P

I

E

S

M

T

V

A

K

K

E

V

V

E

L

E

N

L

V

I

M

H

K

E

Q

L

L

K

A

K

N

D

E

-

G

Y

M

T

T

I

M

V

V

I

V

V

V

T

T

H
|
H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

D

D

K

R

T

V

A

I

F

F

F

M

Y

D

L

D

G

G

E

V

M

I

V

V

E

E

Y

E

D

G

D

T

T

P

K

E

I

I

F

F

T

Y

N

R

P

A

E

K

K

N

E

E

A

R

T

T

Q

R

N

E

Y

F

I

L

T

S

binding adenosine-5'-triphosphate: F11 (≠ Y15), V16 (≠ A20), S36 (= S40), G37 (= G41), S38 (≠ C42), G39 (= G43), K40 (= K44), S41 (= S45), T42 (= T46), E162 (= E174), H194 (= H205)
binding magnesium ion: S41 (= S45), E162 (= E174)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
35% identity, 91% coverage: 18:247/252 of query aligns to 18:241/343 of P30750

query
sites
P30750

F

I

H

Q

A

A

L

L

K

N

G

N

I

V

S

S

M

L

Q

H

I

V

E

P

E

A

K

G

S

Q

V

I

V

Y

A

G

F

V

I

I

G

G

P

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

L

C

F

V

N

N

R

L

M

L

N

E

D

R

L

-

I

-

P

P

A

T

T

-

R

-

L

-

E

E

G

G

E

S

I

V

R

L

I

V

D

D

G

G

H

Q

N

E

I

L

Y

T

A

T

K

L

G

S

-

E

V

S

E

E

V

L

D

T

E

K

L

A

R

R

K

R

N

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

R

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

S

T

I

V

F

F

E

G

N

N

V

V

A

A

Y

L

G

P

L

L

R

E

V

L

-

D

N

N

G

T

V

P

K

K

D

D

N

E

A

-

F

-

I

V

R

K

Q

R

R

R

V

V

E

T

E

E

T

L

L

L

K

S

G

L

A

V

A

G

L

L

W

G

D

D

E

-

V

-

K

-

D

-

K

-

L

-

K

K

E

H

S

D

A

S

Y

Y

A

P

-

S

-

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

M

L

A

A

V

S

S

N

P

P

S

K

V

V

L

L

M

C

D

D

E
|
E

P

A

A

T

S

S

A

A

L

L

D

D

P

P

I

A

S

T

T

T

A

R

K

S

V

I

E

L

E

E

L

L

I

L

H

K

E

D

L

I

K

N

K

R

-

R

-

L

D

G

Y

L

T

T

I

I

V

L

I

L

V

I

T

T

H

H

N

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

D

D

K

C

T

V

A

A

F

V

F

I

Y

S

L

N

G

G

E

E

M

L

V

I

E

E

Y

Q

D

D

D

T

T

V

K

S

K

E

I

V

F

F

T

S

N

H

P

P

E

K

K

T

E

P

A

L

T

A

Q

Q

N

K

Y

F

I

I

40:46 (vs. 40:46, 86% identical) binding ATP
E166 (= E174) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
35% identity, 91% coverage: 18:247/252 of query aligns to 19:242/344 of 6cvlD

query
sites
6cvlD

F
x
I

H

Q

A

A

L

L

K

N

G

N

I

V

S

S

M

L

Q

H

I

V

E

P

E

A

K

G

S

Q

V

I

V

Y

A

G

F

V

I

I

G

G

P

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

L

C

F

V

N

N

R

L

M

L

N

E

D

R

L

-

I

-

P

P

A

T

T

-

R

-

L

-

E

E

G

G

E

S

I

V

R

L

I

V

D

D

G

G

H

Q

N

E

I

L

Y

T

A

T

K

L

G

S

-

E

V

S

E

E

V

L

D

T

E

K

L

A

R

R

K

R

N

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

R

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

S

T

I

V

F

F

E

G

N

N

V

V

A

A

Y

L

G

P

L

L

R

E

V

L

-

D

N

N

G

T

V

P

K

K

D

D

N

E

A

-

F

-

I

V

R

K

Q

R

R

R

V

V

E

T

E

E

T

L

L

L

K

S

G

L

A

V

A

G

L

L

W

G

D

D

E

-

V

-

K

-

D

-

K

-

L

-

K

K

E

H

S

D

A

S

Y

Y

A

P

-

S

-
x
N

L

L

S
|
S

G

G

Q
|
Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

M

L

A

A

V

S

S

N

P

P

S

K

V

V

L

L

M

C

D

D

E

Q

P

A

A

T

S

S

A

A

L

L

D

D

P

P

I

A

S

T

T

T

A

R

K

S

V

I

E

L

E

E

L

L

I

L

H

K

E

D

L

I

K

N

K

R

-

R

-

L

D

G

Y

L

T

T

I

I

V

L

I

L

V

I

T

T

H
|
H

N

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

D

D

K

C

T

V

A

A

F

V

F

I

Y

S

L

N

G

G

E

E

M

L

V

I

E

E

Y

Q

D

D

D

T

T

V

K

S

K

E

I

V

F

F

T

S

N

H

P

P

E

K

K

T

E

P

A

L

T

A

Q

Q

N

K

Y

F

I

I

binding phosphothiophosphoric acid-adenylate ester: I19 (≠ F18), S41 (= S40), G42 (= G41), A43 (≠ C42), G44 (= G43), K45 (= K44), S46 (= S45), T47 (= T46), N141 (vs. gap), S143 (= S150), Q146 (= Q153), H200 (= H205)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12, 14

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
35% identity, 91% coverage: 18:247/252 of query aligns to 19:242/344 of 3tuzC

query
sites
3tuzC

F

I

H

Q

A

A

L

L

K

N

G

N

I

V

S

S

M

L

Q

H

I

V

E

P

E

A

K

G

S

Q

V

I

V

Y

A

G

F

V

I

I

G

G

P

A

S

S

G
|
G

C

A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

L

C

F

V

N

N

R

L

M

L

N

E

D

R

L

-

I

-

P

P

A

T

T

-

R

-

L

-

E

E

G

G

E

S

I

V

R

L

I

V

D

D

G

G

H

Q

N

E

I

L

Y

T

A

T

K

L

G

S

-

E

V

S

E

E

V

L

D

T

E

K

L

A

R

R

K

R

N

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

R

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

S

T

I

V

F

F

E

G

N

N

V

V

A

A

Y

L

G

P

L

L

R

E

V

L

-

D

N

N

G

T

V

P

K

K

D

D

N

E

A

-

F

-

I

V

R

K

Q

R

R

R

V

V

E

T

E

E

T

L

L

L

K

S

G

L

A

V

A

G

L

L

W

G

D

D

E

-

V

-

K

-

D

-

K

-

L

-

K

K

E

H

S

D

A

S

Y

Y

A

P

-

S

-

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

M

L

A

A

V

S

S

N

P

P

S

K

V

V

L

L

M

C

D

D

E

Q

P

A

A

T

S

S

A

A

L

L

D

D

P

P

I

A

S

T

T

T

A

R

K

S

V

I

E

L

E

E

L

L

I

L

H

K

E

D

L

I

K

N

K

R

-

R

-

L

D

G

Y

L

T

T

I

I

V

L

I

L

V

I

T

T

H

H

N

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

D

D

K

C

T

V

A

A

F

V

F

I

Y

S

L

N

G

G

E

E

M

L

V

I

E

E

Y

Q

D

D

D

T

T

V

K

S

K

E

I

V

F

F

T

S

N

H

P

P

E

K

K

T

E

P

A

L

T

A

Q

Q

N

K

Y

F

I

I

binding adenosine-5'-diphosphate: G42 (= G41), G44 (= G43), K45 (= K44), S46 (= S45), T47 (= T46)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 14
binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
35% identity, 91% coverage: 18:247/252 of query aligns to 19:242/344 of 3tuiC

query
sites
3tuiC

F

I

H

Q

A

A

L

L

K

N

G

N

I

V

S

S

M

L

Q

H

I

V

E

P

E

A

K

G

S

Q

V

I

V

Y

A

G

F

V

I

I

G

G

P

A

S

S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

L

C

F

V

N

N

R

L

M

L

N

E

D

R

L

-

I

-

P

P

A

T

T

-

R

-

L

-

E

E

G

G

E

S

I

V

R

L

I

V

D

D

G

G

H

Q

N

E

I

L

Y

T

A

T

K

L

G

S

-

E

V

S

E

E

V

L

D

T

E

K

L

A

R

R

K

R

N

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

R

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

S

T

I

V

F

F

E

G

N

N

V

V

A

A

Y

L

G

P

L

L

R

E

V

L

-

D

N

N

G

T

V

P

K

K

D

D

N

E

A

-

F

-

I

V

R

K

Q

R

R

R

V

V

E

T

E

E

T

L

L

L

K

S

G

L

A

V

A

G

L

L

W

G

D

D

E

-

V

-

K

-

D

-

K

-

L

-

K

K

E

H

S

D

A

S

Y

Y

A

P

-

S

-

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

M

L

A

A

V

S

S

N

P

P

S

K

V

V

L

L

M

C

D

D

E

Q

P

A

A

T

S

S

A

A

L

L

D

D

P

P

I

A

S

T

T

T

A

R

K

S

V

I

E

L

E

E

L

L

I

L

H

K

E

D

L

I

K

N

K

R

-

R

-

L

D

G

Y

L

T

T

I

I

V

L

I

L

V

I

T

T

H

H

N

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

D

D

K

C

T

V

A

A

F

V

F

I

Y

S

L

N

G

G

E

E

M

L

V

I

E

E

Y

Q

D

D

D

T

T

V

K

S

K

E

I

V

F

F

T

S

N

H

P

P

E

K

K

T

E

P

A

L

T

A

Q

Q

N

K

Y

F

I

I

binding adenosine-5'-diphosphate: G42 (= G41), A43 (≠ C42), G44 (= G43), K45 (= K44), S46 (= S45), T47 (= T46)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
37% identity, 88% coverage: 25:247/252 of query aligns to 46:263/382 of 7ahhC

query
sites
7ahhC

S

N

M

F

Q

E

I

I

E

N

E

E

K

G

S

E

V

I

V

F

A

V

F

I

I

M

G

G

P

L

S

S

G
|
G

C

S

G
|
G

K
|
K

S

S

T

T

F

L

L

L

R

R

L

L

F

L

N

N

R

R

M

-

N

-

D

-

L

L

I

I

P

E

A

P

T

T

R

-

L

-

E

S

G

G

E

K

I

I

R

F

I

I

D

D

G

N

H

Q

N

D

I

V

Y

A

A

T

K

L

G

N

V

K

E

E

-

D

-

L

V

L

D

Q

E

V

L

R

R

R

K

K

N

T

V

M

G

S

M

M

V

V

F

F

Q

Q

R

N

P

F

N

G

P

L

F

F

P

P

-

H

K

R

S

T

I

I

F

L

E

E

N

N

V

T

A

E

Y

Y

G

G

L

L

R

E

V

V

N

Q

G

N

V

V

K

P

D

K

N

E

A

E

F

R

I

-

R

R

Q

K

R

R

V

A

E

E

E

K

T

A

L

L

K

D

G

N

A

A

A

N

L

L

W

L

D

D

E

-

V

F

K

K

D

D

K

Q

L

Y

K

P

E

K

S

Q

A

-

Y

-

A

-

L

L

S

S

G

G

Q

M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

M

L

A

A

V

N

S

D

P

P

S

E

V

I

L

L

M

M

D
|
D

E
|
E

P

A

A

F

S

S

A

A

L

L

D

D

P

P

I

L

S

I

T

R

A

R

K

E

V

M

E

Q

E

D

L

E

I

L

H

L

E

E

L

L

K

Q

K

A

D

K

Y

F

-

Q

-

K

T

T

I

I

V

I

I

F

V

V

T

S

H

H

N

D

M

L

Q

N

Q

E

A

A

A

L

R

R

V

I

S

G

D

D

K

R

T

I

A

A

F

I

F

M

Y

K

L

D

G

G

E

K

M

I

V

M

E

Q

Y

I

D

G

D

T

T

G

K

E

I

I

F

L

T

T

N

N

P

P

E

A

K

N

E

D

A

Y

T

V

Q

K

N

T

Y

F

I

V

binding phosphoaminophosphonic acid-adenylate ester: G62 (= G41), G64 (= G43), K65 (= K44), D187 (= D173), E188 (= E174)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
37% identity, 88% coverage: 25:247/252 of query aligns to 46:263/382 of 7aheC

query
sites
7aheC

S

N

M

F

Q

E

I

I

E

N

E

E

K

G

S

E

V

I

V

F

A

V

F

I

I

M

G

G

P

L

S

S

G

G

C

S

G

G

K

K

S

S

T

T

F

L

L

L

R

R

L

L

F

L

N

N

R

R

M

-

N

-

D

-

L

L

I

I

P

E

A

P

T

T

R

-

L

-

E

S

G

G

E

K

I

I

R

F

I

I

D

D

G

N

H

Q

N

D

I

V

Y

A

A

T

K

L

G

N

V

K

E

E

-

D

-

L

V

L

D

Q

E

V

L

R

R

R

K

K

N

T

V

M

G

S

M

M

V

V

F

F

Q

Q

R

N

P

F

N

G

P

L

F

F

P

P

-

H

K

R

S

T

I

I

F

L

E

E

N

N

V

T

A

E

Y

Y

G

G

L

L

R

E

V

V

N

Q

G

N

V

V

K

P

D

K

N

E

A

E

F

R

I

-

R

R

Q

K

R

R

V

A

E

E

E

K

T

A

L

L

K

D

G

N

A

A

A

N

L

L

W

L

D

D

E

-

V

F

K

K

D

D

K

Q

L

Y

K

P

E

K

S

Q

A

-

Y

-

A

-

L

L

S

S

G

G

Q

M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

M

L

A

A

V

N

S

D

P

P

S

E

V

I

L

L

M

M

D

D

E

E

P

A

A

F

S

S

A

A

L

L

D

D

P

P

I

L

S

I

T

R

A

R

K

E

V

M

E

Q

E

D

L

E

I

L

H

L

E

E

L

L

K

Q

K

A

D

K

Y

F

-

Q

-

K

T

T

I

I

V

I

I

F

V

V

T

S

H

H

N

D

M

L

Q

N

Q

E

A

A

A

L

R

R

V

I

S

G

D

D

K

R

T

I

A

A

F

I

F

M

Y

K

L

D

G

G

E

K

M

I

V

M

E

Q

Y

I

D

G

D

T

T

G

K

E

I

I

F

L

T

T

N

N

P

P

E

A

K

N

E

D

A

Y

T

V

Q

K

N

T

Y

F

I

V

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298

7ahdC Opua (e190q) occluded (see paper)
38% identity, 85% coverage: 25:238/252 of query aligns to 46:254/260 of 7ahdC

query
sites
7ahdC

S

N

M

F

Q

E

I

I

E

N

E

E

K

G

S

E

V

I

V

F

A

V

F

I

I

M

G

G

P

L

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

R

L

L

F

L

N

N

R

R

M

-

N

-

D

-

L

L

I

I

P

E

A

P

T

T

R

-

L

-

E

S

G

G

E

K

I

I

R

F

I

I

D

D

G

N

H

Q

N

D

I

V

Y

A

A

T

K

L

G

N

V

K

E

E

-

D

-

L

V

L

D

Q

E

V

L

R

R

R

K

K

N

T

V

M

G

S

M

M

V

V

F

F

Q
|
Q

R

N

P

F

N

G

P

L

F

F

P

P

-

H

K

R

S

T

I

I

F

L

E

E

N

N

V

T

A

E

Y

Y

G

G

L

L

R

E

V

V

N

Q

G

N

V

V

K

P

D

K

N

E

A

E

F

R

I

-

R

R

Q

K

R

R

V

A

E

E

E

K

T

A

L

L

K

D

G

N

A

A

A

N

L

L

W

L

D

D

E

-

V

F

K

K

D

D

K

Q

L

Y

K

P

E
x
K

S
x
Q

A

-

Y

-

A

-

L

L

S
|
S

G

G

G
|
G

Q
x
M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

M

L

A

A

V

N

S

D

P

P

S

E

V

I

L

L

M

M

D

D

E
x
Q

P

A

A

F

S

S

A

A

L

L

D

D

P

P

I

L

S

I

T

R

A

R

K

E

V

M

E

Q

E

D

L

E

I

L

H

L

E

E

L

L

K

Q

K

A

D

K

Y

F

-

Q

-

K

T

T

I

I

V

I

I

F

V

V

T

S

H
|
H

N

D

M

L

Q

N

Q

E

A

A

A

L

R

R

V

I

S

G

D

D

K

R

T

I

A

A

F

I

F

M

Y

K

L

D

G

G

E

K

M

I

V

M

E

Q

Y

I

D

G

D

T

T

G

K

E

I

I

F

L

T

T

N

N

P

P

binding adenosine-5'-triphosphate: S61 (= S40), G62 (= G41), G64 (= G43), K65 (= K44), S66 (= S45), T67 (= T46), Q111 (= Q93), K161 (≠ E144), Q162 (≠ S145), S164 (= S150), G166 (= G152), M167 (≠ Q153), Q188 (≠ E174), H221 (= H205)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 39

P21439 Phosphatidylcholine translocator ABCB4; ATP-binding cassette sub-family B member 4; Multidrug resistance protein 3; P-glycoprotein 3; EC 7.6.2.1 from Homo sapiens (Human) (see 22 papers)
35% identity, 90% coverage: 2:227/252 of query aligns to 388:610/1286 of P21439

query
sites
P21439

D

D

T

S

V

I

K

K

-

G

-

N

I

L

D

E

T

F

R

N

D

D

V

V

N

H

F

F

W

S

Y
|
Y

-

P

-

S

-
x
R

G

A

D

N

F

V

H

K

A

I

L

L

K

K

G

G

I

L

S

N

M

L

Q

K

I

V

E

Q

E

S

K

G

S

Q

V

T

V

V

A

A

F

L

I

V

G

G

P

S

S

S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

F

T

L

V

R

Q

L

L

F

I

N

Q

R

R

M

L

N

Y

D

D

L

-

I

-

P

P

A

D

T

-

R

-

L

-

E
|
E

G

G

E

T

I

I

R

N

I

I

D

D

G

G

H

Q

N
x
D

I

I

Y

-

A

-

K

R

G

N

V

F

E

N

V

V

D

N

E

Y

L

L

R

R

K

E

N

I

V

I

G

G

M

V

V

V

F

S

Q
|
Q

R

E

P
|
P

N

V

P
x
L

F

F

P

S

K

T

S

T

I

I

F

A

E

E

N

N

V

I

A

C

Y

Y

G

G

L

-

R

R

V

G

N

N

G

V

V

T

K

M

D

D

N

E

A

-

F

-

I

I

R

K

Q

K

R

A

V

V

E

K

E

E

T

A

-

N

-
x
A

-

Y

-

E

-

F

-

I

L

M

K

K

G

L

A

P

A

Q

L

K

W

F

D

D

E

T

V

L

K

-

D

-

K

-

L

V

K

G

E
|
E

S

R

A

G

Y

A

A

Q

L

L

S

S

G
|
G

Q

Q

Q

K

Q

Q

R

R

L
x
I

C

A

I

I

A

A

R

R

A
|
A

M

L

A

V

V

R

S

N

P

P

S

K

V

I

L

L

M

L

D

D

E
|
E

P

A

A

T

S

S

A

A

L

L

D

D

P

T

I

E

S

S

T

E

A

A

K

E

V

V

E

Q

E

A

L

A

I

L

H

D

E

K

L

A

K

R

K

E

D

G

Y

R

T

T

I

T

V

I

I

V

V

I

T

A

H
|
H

N
x
R

M

L

Q

-

Q

S

A

T

A

V

R

R

V

N

S

A

D

D

K

V

T

I

A

A

F

G

F

F

Y

E

L

D

G

G

E

V

M

I

V

V

E

E

Y403 (= Y15) to H: in PFIC3; does not alter cytoplasmic and cell membrane location; inhibits efflux activity for PC and cholesterol; dbSNP:rs121918443
R406 (vs. gap) binding ATP; to Q: found in patients with cholangitis; uncertain significance; dbSNP:rs763807769
GCGKST 432:437 (= GCGKST 41:46) binding ATP
K435 (= K44) mutation to M: Inhibits efflux activity for PC and cholesterol, but does not alter glycosylation and surface expression in the presence of taurocholate.
E450 (= E64) to G: in dbSNP:rs1189003716
D459 (≠ N73) to H: in PFIC3; retained in the reticulum endoplasmic; greatly reduced expression
Q477 (= Q93) binding ATP
P479 (= P95) to L: in PFIC3; greatly reduced expression; alters efflux activity for PC; dbSNP:rs748657435
L481 (≠ P97) to R: in PFIC3; does not alter cytoplasmic and cell membrane location; inhibits efflux activity for PC and cholesterol
A511 (vs. gap) to T: in PFIC3 and GBD1; dbSNP:rs1257887155
E528 (= E144) to D: in GBD1; uncertain significance; moderate decrease of phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs8187797
G535 (= G151) to D: in PFIC3; reduced phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs1810849139
G536 (= G152) binding ATP; to R: in GBD1; loss of phosphatidylcholine transporter activity; does not alter plasma membrane location
I541 (≠ L157) to F: in PFIC3 and GBD1; dbSNP:rs66904256
A546 (= A162) to D: in ICP3; disruption of protein trafficking with subsequent lack of functional protein at the cell surface; dbSNP:rs121918441
E558 (= E174) mutation to Q: Loss of floppase activity. Strongly reduce the ATPase activity.
H589 (= H205) to T: in GBD1; requires 2 nucleotide substitutions
R590 (≠ N206) to Q: found in patients with gallbladder and cholestasis; uncertain significance; dbSNP:rs45575636

Sites not aligning to the query:

34 modified: Phosphothreonine; T → M: in GBD1; reduces efflux activity for PC in a phosphorylation-dependent manner; dbSNP:rs142794414; T→D: Does not inhibit efflux activity for PC.
44 T→A: Reduces efflux activity for PC. Does not alter apical membrane location.
47 R → G: in GBD1; partly retained intracellularly; reduces efflux activity for PC in a phosphorylation-dependent manner; R → Q: found in patients with cholangitis; uncertain significance; dbSNP:rs372685632
49 S→A: Reduces efflux activity for PC. Does not alter apical membrane location.
68 G → R: in PFIC3; retained in the reticulum endoplasmic; greatly reduced expression; dbSNP:rs1343667900
73 L → V: in PFIC3 and GBD1; dbSNP:rs8187788
87 natural variant: D -> E
95 P → S: in dbSNP:rs377268767
175 T → A: found in patients with gallbladder and cholestasis; uncertain significance; dbSNP:rs58238559
201 T → M: in PFIC3; greatly reduced expression; alters efflux activity for PC; dbSNP:rs753318087
238 L → V: in dbSNP:rs45596335
263 I → V: in dbSNP:rs45547936
286 A → V: in PFIC3 and GBD1; does not alter plasma membrane location; inhibits efflux activity for PC; dbSNP:rs765478923
320 S → F: in ICP3, GBD1 and PFIC3; uncertain significance; does not alter plasma membrane location; does not inhibit efflux activity for PC; dbSNP:rs72552778
367 I → V: in dbSNP:rs1168923653
651 T → N: in dbSNP:rs45476795
652 R → G: in dbSNP:rs2230028
726 P → L: in GBD1; loss of phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs141677867
742 natural variant: G -> S
764 I → L: in a heterozygous patient with risperidone-induced cholestasis
775 T → M: found in patients with cholangitis; uncertain significance; dbSNP:rs148052192
788 R → Q: in GBD1; benign; dbSNP:rs8187801
934 A → T: found in patients with gallbladder and cholestasis; uncertain significance; dbSNP:rs61730509
953 A→D: Accumulates predominantly in intracellular compartments with only a small fraction at the plasma membrane and inhibits partially the efflux activity for PC.
964 V → T: found in patients with cholangitis; uncertain significance; requires 2 nucleotide substitutions
978 S → P: in PFIC3; alters efflux activity for PC; dbSNP:rs1051861187
985 V→M: Significantly reduces phosphatidylcholine floppase activity; when associated with Q-989 and V-990.
989 H→Q: Significantly reduces phosphatidylcholine floppase activity; when associated with M-985 and V-990.
990 A→V: Significantly reduces phosphatidylcholine floppase activity; when associated with M-985 and Q-989.
1046 binding ATP
1071:1077 binding ATP
1075 K→M: Inhibits efflux activity for PC and cholesterol, but does not alter glycosylation and surface expression in the presence of taurocholate.
1082 L → Q: in a heterozygous patient with amoxicillin/clavulanic acid-induced cholestasis; dbSNP:rs1214110864
1124 binding ATP
1125 E → K: in PFIC3; alters efflux activity for PC
1168 P → S: in GBD1; reduced phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs121918442
1183 S → L: in GBD1; severely reduced phosphatidylcholine transporter activity; does not alter plasma membrane location
1184:1186 binding ATP
1185 G → S: in GBD1; loss of phosphatidylcholine transporter activity; does not alter plasma membrane location

7niwA Nanodisc reconstituted human abcb4 in complex with 4b1-fab (posaconazole-bound, inward-open conformation) (see paper)
35% identity, 90% coverage: 2:227/252 of query aligns to 337:559/1140 of 7niwA

query
sites
7niwA

D

D

T

S

V

I

K

K

-

G

-

N

I

L

D

E

T

F

R

N

D

D

V

V

N

H

F

F

W

S

Y

Y

-

P

-

S

-

R

G

A

D

N

F

V

H

K

A

I

L

L

K

K

G

G

I

L

S

N

M

L

Q

K

I

V

E

Q

E

S

K

G

S

Q

V

T

V

V

A

A

F

L

I

V

G

G

P

S

S

S

G

G

C

C

G

G

K

K

S

S

T

T

F

T

L

V

R

Q

L

L

F

I

N

Q

R

R

M

L

N

Y

D

D

L

-

I

-

P

P

A

D

T

-

R

-

L

-

E

E

G

G

E

T

I

I

R

N

I

I

D

D

G

G

H

Q

N

D

I

I

Y

-

A

-

K

R

G

N

V

F

E

N

V

V

D

N

E

Y

L

L

R

R

K

E

N

I

V

I

G

G

M

V

V

V

F

S

Q

Q

R

E

P

P

N

V

P

L

F

F

P

S

K

T

S

T

I

I

F

A

E

E

N

N

V

I

A

C

Y

Y

G

G

L

-

R

R

V

G

N

N

G

V

V

T

K

M

D

D

N

E

A

-

F

-

I

I

R

K

Q

K

R

A

V

V

E

K

E

E

T

A

-

N

-

A

-

Y

-

E

-

F

-

I

L

M

K

K

G

L

A

P

A

Q

L

K

W

F

D

D

E

T

V

L

K

-

D

-

K

-

L

V

K

G

E

E

S

R

A

G

Y

A

A

Q

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

A

A

M

L

A

V

V

R

S

N

P

P

S

K

V

I

L

L

M

L

D

D

E

Q

P

A

A

T

S

S

A

A

L

L

D

D

P

T

I

E

S

S

T

E

A

A

K

E

V

V

E

Q

E

A

L

A

I

L

H

D

E

K

L

A

K

R

K

E

D

G

Y

R

T

T

I

T

V

I

I

V

V

I

T

A

H

H

N

R

M

L

Q

-

Q

S

A

T

A

V

R

R

V

N

S

A

D

D

K

V

T

I

A

A

F

G

F

F

Y

E

L

D

G

G

E

V

M

I

V

V

E

E

Sites not aligning to the query:

binding cholesterol: 70, 152, 160, 266, 641, 645, 648, 839
binding 1,2-dilinoleoyl-sn-glycero-3-phosphocholine: 173, 183, 250, 254, 257, 261, 286, 294, 298, 617, 882
binding posaconazole: 250, 291, 294, 658, 661, 726, 841, 871, 874, 878, 882

6s7pA Nucleotide bound abcb4 (see paper)
35% identity, 90% coverage: 2:227/252 of query aligns to 327:549/1128 of 6s7pA

query
sites
6s7pA

D

D

T

S

V

I

K

K

-

G

-

N

I

L

D

E

T

F

R

N

D

D

V

V

N

H

F

F

W

S

Y
|
Y

-

P

-
x
S

-
x
R

G

A

D

N

F

V

H

K

A

I

L

L

K

K

G

G

I

L

S

N

M

L

Q

K

I

V

E

Q

E

S

K

G

S

Q

V

T

V

V

A

A

F

L

I

V

G

G

P

S

S

S

G
|
G

C

C

G
|
G

K
|
K

S
|
S

T

T

F

T

L

V

R

Q

L

L

F

I

N

Q

R

R

M

L

N

Y

D

D

L

-

I

-

P

P

A

D

T

-

R

-

L

-

E

E

G

G

E

T

I

I

R

N

I

I

D

D

G

G

H

Q

N

D

I

I

Y

-

A

-

K

R

G

N

V

F

E

N

V

V

D

N

E

Y

L

L

R

R

K

E

N

I

V

I

G

G

M

V

V

V

F

S

Q
|
Q

R

E

P

P

N

V

P

L

F

F

P

S

K

T

S

T

I

I

F

A

E

E

N

N

V

I

A

C

Y

Y

G

G

L

-

R

R

V

G

N

N

G

V

V

T

K

M

D

D

N

E

A

-

F

-

I

I

R

K

Q

K

R

A

V

V

E

K

E

E

T

A

-

N

-

A

-

Y

-

E

-

F

-

I

L

M

K

K

G

L

A

P

A

Q

L

K

W

F

D

D

E

T

V

L

K

-

D

-

K

-

L

V

K

G

E

E

S

R

A

G

Y

A

A
x
Q

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

A

A

M

L

A

V

V

R

S

N

P

P

S

K

V

I

L

L

M

L

D

D

E

Q

P

A

A

T

S

S

A

A

L

L

D

D

P

T

I

E

S

S

T

E

A

A

K

E

V

V

E

Q

E

A

L

A

I

L

H

D

E

K

L

A

K

R

K

E

D

G

Y

R

T

T

I

T

V

I

I

V

V

I

T

A

H

H

N

R

M

L

Q

-

Q

S

A

T

A

V

R

R

V

N

S

A

D

D

K

V

T

I

A

A

F

G

F

F

Y

E

L

D

G

G

E

V

M

I

V

V

E

E

binding adenosine-5'-triphosphate: Y342 (= Y15), S344 (vs. gap), R345 (vs. gap), G371 (= G41), G373 (= G43), K374 (= K44), S375 (= S45), Q416 (= Q93), Q471 (≠ A148), S473 (= S150), G475 (= G152), Q476 (= Q153)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 920, 922, 923, 948, 949, 951, 952, 953, 954, 994, 1033, 1053, 1054, 1056
binding cholesterol: 25, 32, 35, 65, 824, 834, 834, 838
binding magnesium ion: 953, 994

P02915 Histidine/lysine/arginine/ornithine transport ATP-binding protein HisP; EC 7.4.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
35% identity, 100% coverage: 1:251/252 of query aligns to 2:257/258 of P02915

query
sites
P02915

M

M

D

S

T

E

V

N

K

K

I

L

D

H

T

V

R

I

D

D

V

L

N

H

F

K

W

R

Y

Y

G

G

D

G

F

H

H

E

A

V

L

L

K

K

G

G

I

V

S

S

M

L

Q

Q

I

A

E

R

E

A

K

G

S

D

V

V

V

I

A

S

F

I

I

I

G

G

P

S

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

L

C

F

I

N

N

R

F

M

L

N

E

D

K

L

-

I

-

P

P

A

S

T

-

R

-

L

-

E

E

G

G

E

A

I

I

R

I

I

V

D

N

G

G

H

Q

N

N

I

I

-

N

-

L

-

V

-

R

-

D

-

K

-

D

-

G

-

Q

-

L

-

K

Y

V

A

A

K

D

G

K

V

N

E

Q

V

L

D

R

E

L

L

L

R

R

K

T

N

R

V

L

G

T

M

M

V

V

F

F

Q

Q

R

H

P

F

N

N

P

L

F

W

P

S

K

H

-

M

S

T

I

V

F

L

E

E

N

N

V

V

A

M

Y

E

G

A

-

P

L

I

R

Q

V

V

N

L

G

G

V

L

-

S

K

K

D

H

N

D

A

A

F

-

I

-

R

R

Q

E

R

R

V

A

E

L

E

K

T

Y

L

L

K

A

G

K

A

V

A

G

L

I

W

D

D

E

E

R

V

A

K

Q

D

G

K

K

L

Y

K

-

E

-

S

-

A

P

Y

V

A

H

L

L

S

S

G

G

Q

Q

R

R

L

V

C

S

I

I

A

A

R

R

A

A

M

L

A

A

V

M

S

E

P

P

S

D

V

V

L

L

M

F

D

D

E

E

P

P

A

T

S

S

A

A

L

L

D

D

P

P

I

E

S

L

T

V

A

G

K

E

V

V

E

L

E

R

L

I

I

M

H

Q

E

Q

L

L

K

A

K

E

D

E

-

G

Y

K

T

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

H

V

V

S

S

D

S

K

H

T

V

A

I

F

F

F

L

Y

H

L

Q

G

G

E

K

M

I

V

E

E

E

Y

E

D

G

D

D

T

P

K

E

K

Q

I

V

F

F

T

G

N

N

P

P

E

Q

K

S

E

P

A

R

T

L

Q

Q

N

Q

Y

F

I

L

T

K

G

G

R

S

F

L

S41 (= S40) binding ATP
G42 (= G41) binding ATP
G44 (= G43) binding ATP
K45 (= K44) binding ATP
S46 (= S45) binding ATP
T47 (= T46) binding ATP

6qexA Nanodisc reconstituted human abcb1 in complex with uic2 fab and taxol (see paper)
35% identity, 90% coverage: 2:227/252 of query aligns to 355:577/1182 of 6qexA

query
sites
6qexA

D

D

T

N

V

I

K

K

-

G

-

N

I

L

D

E

T

F

R

R

D

N

V

V

N

H

F

F

W

S

Y

Y

-

P

-

S

-

R

G

K

D

E

F

V

H

K

A

I

L

L

K

K

G

G

I

L

S

N

M

L

Q

K

I

V

E

Q

E

S

K

G

S

Q

V

T

V

V

A

A

F

L

I

V

G

G

P

N

S

S

G

G

C

C

G

G

K

K

S

S

T

T

F

T

L

V

R

Q

L

L

F

M

N

Q

R

R

M

L

N

Y

D

D

L

-

I

-

P

P

A

T

T

-

R

-

L

-

E

E

G

G

E

M

I

V

R

S

I

V

D

D

G

G

H

Q

N

D

I

I

Y

-

A

-

K

R

G

T

V

I

E

N

V

V

D

R

E

F

L

L

R

R

K

E

N

I

V

I

G

G

M

V

V

V

F

S

Q

Q

R

E

P

P

N

V

P

L

F

F

P

A

K

T

S

T

I

I

F

A

E

E

N

N

V

I

A

R

Y

Y

G

G

L

-

R

R

V

E

N

N

G

V

V

T

K

M

D

D

N

-

A

-

F

-

I

-

R

-

Q

-

R

E

V

I

E

E

E

K

T

A

L

V

K

K

G

E

A

A

A

N

L

A

W

Y

D

D

E

F

V

I

K

M

-

K

-

L

-

P

-

H

-

K

-

F

D

D

K

T

L

L

-

V

K

G

E

E

S

R

A

G

Y

A

A

Q

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

I

C

A

I

I

A

A

R

R

A

A

M

L

A

V

V

R

S

N

P

P

S

K

V

I

L

L

M

L

D

D

E

E

P

A

A

T

S

S

A

A

L

L

D

D

P

T

I

E

S

S

T

E

A

A

K

V

V

V

E

Q

E

V

L

A

I

L

H

D

E

K

L

A

K

R

K

K

D

G

Y

R

T

T

I

T

V

I

I

V

V

I

T

A

H

H

N

R

M

L

Q

-

Q

S

A

T

A

V

R

R

V

N

S

A

D

D

K

V

T

I

A

A

F

G

F

F

Y

D

L

D

G

G

E

V

M

I

V

V

E

E

Sites not aligning to the query:

binding cholesterol: 15, 18, 36, 43, 81, 86, 89, 189, 281, 745, 749, 751, 755, 757, 761, 762, 765, 769, 772, 775, 854, 857, 861, 864, 865
binding taxol: 38, 276, 279, 305, 312, 313, 316, 631, 852, 859, 889, 892, 896

1b0uA Atp-binding subunit of the histidine permease from salmonella typhimurium (see paper)
36% identity, 97% coverage: 5:249/252 of query aligns to 2:251/258 of 1b0uA

query
sites
1b0uA

K

K

I

L

D

H

T

V

R

I

D

D

V

L

N

H

F

K

W

R

Y
|
Y

G

G

D

G

F
x
H

H

E

A

V

L

L

K

K

G

G

I

V

S

S

M

L

Q

Q

I

A

E

R

E

A

K

G

S

D

V

V

V

I

A

S

F

I

I

I

G

G

P

S

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

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binding adenosine-5'-triphosphate: Y12 (= Y15), H15 (≠ F18), S37 (= S40), G38 (= G41), G40 (= G43), K41 (= K44), S42 (= S45), T43 (= T46)

Query Sequence

>350849 FitnessBrowser__Btheta:350849
MDTVKIDTRDVNFWYGDFHALKGISMQIEEKSVVAFIGPSGCGKSTFLRLFNRMNDLIPA
TRLEGEIRIDGHNIYAKGVEVDELRKNVGMVFQRPNPFPKSIFENVAYGLRVNGVKDNAF
IRQRVEETLKGAALWDEVKDKLKESAYALSGGQQQRLCIARAMAVSPSVLLMDEPASALD
PISTAKVEELIHELKKDYTIVIVTHNMQQAARVSDKTAFFYLGEMVEYDDTKKIFTNPEK
EATQNYITGRFG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory