SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 351108 FitnessBrowser__Btheta:351108 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 6 hits to proteins with known functional sites (download)

2c21A Specificity of the trypanothione-dependednt leishmania major glyoxalase i: structure and biochemical comparison with the human enzyme (see paper)
30% identity, 95% coverage: 6:125/126 of query aligns to 3:123/139 of 2c21A

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active site: H6 (= H9), E57 (= E58), H75 (= H77), E118 (= E120)
binding nickel (ii) ion: H6 (= H9), E57 (= E58), H75 (= H77), E118 (= E120)

P0AC81 Lactoylglutathione lyase; Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase; EC 4.4.1.5 from Escherichia coli (strain K12) (see paper)
33% identity, 95% coverage: 6:125/126 of query aligns to 2:127/135 of P0AC81

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H5 (= H9) binding
E56 (= E58) binding
H74 (= H77) binding in other chain
E122 (= E120) binding in other chain

4mttA Ni- and zn-bound gloa2 at low resolution (see paper)
24% identity, 94% coverage: 6:124/126 of query aligns to 2:126/128 of 4mttA

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4mttA

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active site: H5 (= H9), E56 (= E58), H74 (= H77), E122 (= E120)
binding zinc ion: H5 (= H9), E56 (= E58)

1fa6A Crystal structure of the co(ii)-bound glyoxalase i of escherichia coli (see paper)
30% identity, 94% coverage: 6:124/126 of query aligns to 2:126/128 of 1fa6A

query
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1fa6A

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active site: H5 (= H9), E56 (≠ L56), H74 (= H77), E122 (= E120)
binding cobalt (ii) ion: H5 (= H9), E56 (≠ L56), H74 (= H77), E122 (= E120)

1fa5A Crystal structure of the zn(ii)-bound glyoxalase i of escherichia coli (see paper)
30% identity, 94% coverage: 6:124/126 of query aligns to 2:126/128 of 1fa5A

query
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1fa5A

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active site: H5 (= H9), E56 (≠ L56), H74 (= H77), E122 (= E120)
binding zinc ion: H5 (= H9), E56 (≠ L56), H74 (= H77), E122 (= E120)

P50107 Glyoxalase I; Glx I; Aldoketomutase; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase; actoylglutathione lyase; EC 4.4.1.5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
26% identity, 97% coverage: 3:124/126 of query aligns to 179:322/326 of P50107

query
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P50107

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E318 (= E120) active site, Proton donor/acceptor

Sites not aligning to the query:

163 active site, Proton donor/acceptor

Query Sequence

>351108 FitnessBrowser__Btheta:351108
MEIKSRFDHFNINVTNLERSIAFYEKALGLKEHSRKEASDGSFILVYLTDNETGFLLELT
WLKDHTSPYELGENESHLCFRVAGDYDAIRQYHKEMNCVCFENTAMGLYFINDPDDYWIE
ILPQKK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory