SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 351213 FitnessBrowser__Btheta:351213 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 10 hits to proteins with known functional sites (download)

3oa4A Crystal structure of hypothetical protein bh1468 from bacillus halodurans c-125
45% identity, 99% coverage: 2:133/134 of query aligns to 1:131/138 of 3oa4A

query
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3oa4A

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active site: H7 (= H8), I43 (≠ T44), E54 (= E55), H78 (= H79), E128 (= E130)
binding zinc ion: H7 (= H8), E54 (= E55), H78 (= H79), E128 (= E130)

6wfhA Streptomyces coelicolor methylmalonyl-coa epimerase substrate complex (see paper)
38% identity, 97% coverage: 2:131/134 of query aligns to 1:135/139 of 6wfhA

query
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6wfhA

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active site: H7 (= H8), E43 (≠ T44), Q60 (≠ E55), H84 (= H79), E134 (= E130)
binding cobalt (ii) ion: H7 (= H8), Q60 (≠ E55), H84 (= H79), E134 (= E130)
binding (3S,5R,9R,19E)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9,19-tetrahydroxy-8,8,20-trimethyl-10,14-dioxo-2,4,6-trioxa-18-thia-11,15-diaza-3,5-diphosphahenicos-19-en-21-oic acid 3,5-dioxide (non-preferred name): H7 (= H8), Q39 (= Q40), Q60 (≠ E55), A70 (≠ T65), K73 (= K68), W74 (≠ F69), H83 (= H78), H84 (= H79), L107 (≠ I103), G114 (= G110), S115 (≠ A111), F122 (= F118), P125 (= P121), K126 (= K122), L132 (= L128), E134 (= E130)

6wf6A Streptomyces coelicolor methylmalonyl-coa epimerase (see paper)
38% identity, 97% coverage: 2:131/134 of query aligns to 1:135/141 of 6wf6A

query
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active site: H7 (= H8), E43 (≠ T44), Q60 (≠ E55), H84 (= H79), E134 (= E130)
binding cobalt (ii) ion: H7 (= H8), Q60 (≠ E55), H84 (= H79), E134 (= E130)

6xbqA Streptomyces coelicolor methylmalonyl-coa epimerase in complex with carboxy-carba(dethia)-coa
38% identity, 97% coverage: 2:131/134 of query aligns to 1:135/144 of 6xbqA

query
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6xbqA

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active site: H7 (= H8), E43 (≠ T44), Q60 (≠ E55), H84 (= H79), E134 (= E130)
binding carboxymethyldethia coenzyme *a: Q39 (= Q40), Q60 (≠ E55), A70 (≠ T65), K73 (= K68), W74 (≠ F69), H83 (= H78), H84 (= H79), L107 (≠ I103), F122 (= F118), P125 (= P121), K126 (= K122), L132 (= L128)
binding cobalt (ii) ion: H7 (= H8), Q60 (≠ E55), H84 (= H79), E134 (= E130)

6wfiA Methylmalonyl-coa epimerase in complex with 2-nitronate-propionyl-coa (see paper)
38% identity, 97% coverage: 2:131/134 of query aligns to 1:135/144 of 6wfiA

query
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6wfiA

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active site: H7 (= H8), E43 (≠ T44), Q60 (≠ E55), H84 (= H79), E134 (= E130)
binding cobalt (ii) ion: H7 (= H8), Q60 (≠ E55), H84 (= H79), E134 (= E130)
binding [1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanylidene-propan-2-ylidene]-bis(oxidanidyl)azanium: H7 (= H8), Q39 (= Q40), Q60 (≠ E55), A70 (≠ T65), K73 (= K68), W74 (≠ F69), H83 (= H78), H84 (= H79), L107 (≠ I103), G114 (= G110), S115 (≠ A111), I120 (= I116), F122 (= F118), P125 (= P121), L132 (= L128), E134 (= E130)

6wf7A Methylmalonyl-coa epimerase in complex with methylmalonyl-coa and nh4+ (see paper)
38% identity, 97% coverage: 2:131/134 of query aligns to 1:135/144 of 6wf7A

query
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6wf7A

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active site: H7 (= H8), E43 (≠ T44), Q60 (≠ E55), H84 (= H79), E134 (= E130)
binding (S)-Methylmalonyl-Coenzyme A: Q39 (= Q40), E43 (≠ T44), Q60 (≠ E55), A70 (≠ T65), W74 (≠ F69), H83 (= H78), H84 (= H79), L107 (≠ I103), G114 (= G110), S115 (≠ A111), F122 (= F118), P125 (= P121), K126 (= K122), L132 (= L128), E134 (= E130)
binding methylmalonyl-coenzyme a: Q39 (= Q40), Q60 (≠ E55), A70 (≠ T65), W74 (≠ F69), H83 (= H78), H84 (= H79), L107 (≠ I103), G114 (= G110), S115 (≠ A111), I120 (= I116), F122 (= F118), P125 (= P121), K126 (= K122), L132 (= L128), E134 (= E130)

6xbtA Streptomyces coelicolor methylmalonyl-coa epimerase (q60a) in complex with 2-nitronate-propionyl-coa
38% identity, 97% coverage: 2:131/134 of query aligns to 1:135/140 of 6xbtA

query
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6xbtA

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active site: H7 (= H8), E43 (≠ T44), A60 (≠ E55), H84 (= H79), E134 (= E130)
binding cobalt (ii) ion: H7 (= H8), H84 (= H79), E134 (= E130)
binding coenzyme a: Q39 (= Q40), A70 (≠ T65), K73 (= K68), W74 (≠ F69), K77 (≠ N72), H83 (= H78), L107 (≠ I103), F122 (= F118), P125 (= P121), K126 (= K122), L132 (= L128)
binding [1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanylidene-propan-2-ylidene]-bis(oxidanidyl)azanium: H7 (= H8), Q39 (= Q40), A70 (≠ T65), K73 (= K68), W74 (≠ F69), H83 (= H78), H84 (= H79), L107 (≠ I103), G114 (= G110), S115 (≠ A111), P125 (= P121), K126 (= K122), L132 (= L128), E134 (= E130)

Q96PE7 Methylmalonyl-CoA epimerase, mitochondrial; DL-methylmalonyl-CoA racemase; EC 5.1.99.1 from Homo sapiens (Human) (see paper)
36% identity, 98% coverage: 1:131/134 of query aligns to 43:173/176 of Q96PE7

query
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H50 (= H8) binding
R104 (≠ P62) to L: in dbSNP:rs6748672
H122 (= H79) binding
E172 (= E130) binding

6qh4C Crystal structure of human methylmalonyl-coa epimerase (mcee) p.Arg143cys variant
35% identity, 96% coverage: 3:131/134 of query aligns to 9:135/138 of 6qh4C

query
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active site: H14 (= H8), V50 (≠ T44), E61 (= E55), H86 (= H79), E134 (= E130)
binding cobalt (ii) ion: H14 (= H8), H86 (= H79), E134 (= E130)

2qh0A Crystal structure of a glyoxalase from clostridium acetobutylicum
31% identity, 96% coverage: 6:133/134 of query aligns to 4:128/129 of 2qh0A

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binding zinc ion: H6 (= H8), E53 (= E55), H76 (= H79), E125 (= E130)

Query Sequence

>351213 FitnessBrowser__Btheta:351213
MKISHIEHLGIAVKSIEEALPYYENVLGLKCYNIETVEDQKVRTAFLKVGETKIELLEPT
CPESTIAKFIENKGAGVHHVAFAVEDGVANALAEAESKEIRLIDKAPRKGAEGLNIAFLH
PKSTLGVLTELCEH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory