SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing 351279 BT1751 Glycine betaine transport ATP-binding protein (NCBI ptt file) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
50% identity, 94% coverage: 3:387/408 of query aligns to 2:377/382 of 7ahhC

query
sites
7ahhC

K

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G

C

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x
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Y

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binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: I272 (≠ V280), P273 (≠ K282), L275 (≠ I284), E295 (≠ N304), V296 (≠ L305), S297 (≠ T306), S298 (≠ V307), P357 (= P367), V370 (= V380), I372 (≠ P382)
binding phosphoaminophosphonic acid-adenylate ester: F12 (= F13), K29 (= K30), T39 (= T40), V40 (= V41), G41 (≠ A42), S61 (= S62), G62 (= G63), S63 (= S64), G64 (= G65), K65 (= K66), S66 (= S67), T67 (= T68), D187 (= D188), E188 (= E189)

7aheC Opua inhibited inward facing (see paper)
50% identity, 94% coverage: 3:387/408 of query aligns to 2:377/382 of 7aheC

query
sites
7aheC

K

K

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K

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D

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F

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G

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A

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M

M

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K

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K

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G

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E

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L

L

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K

A

K

T

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G

C

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Y

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D

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N

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E

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G

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E

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G

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binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: L275 (≠ I284), E295 (≠ N304), V296 (≠ L305), S297 (≠ T306), S298 (≠ V307), P357 (= P367), I372 (≠ P382)

7ahdC Opua (e190q) occluded (see paper)
62% identity, 63% coverage: 3:261/408 of query aligns to 2:260/260 of 7ahdC

query
sites
7ahdC

K

K

I

I

E

K

I

I

K

E

D

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L

L

Y

T

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Q

K

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A

A

L

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M

M

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K

E

E

K

G

G

K

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T

P

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K

S

N

E

E

I

I

L

L

K

K

A

K

T

T

G

G

C

A

T
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T

V

V

A

G

V

V

K

Y

D

D

A

T

N

N

L

F

S

E

I

I

N

N

E

E

G

G

E

E

I

I

F

F

V

V

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I

M

M

G

G

L

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S

G
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G

S
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S

G
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G

K
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K

S
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T

L

L

R

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C

L

I

L

N

N

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L

I

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F

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Q

N

N

F

F

G

G

L

L

L

F

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P

H

H

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R

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T

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I

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L

H

E

N

N

I

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A

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Y

G

G

L

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Q

G

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K

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Y

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Y

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S
x
K

E
x
Q

L

L

S
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S

G

G

G
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G

M
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M

Q

Q

R

R

V

V

G

G

L

L

A

A

R

R

A

A

L

L

A

A

N

N

N

D

P

P

E

E

V

I

L

L

M

M

D

D

E
x
Q

A

A

F

F

S

S

A

A

L

L

D

D

P

P

L

L

I

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R

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M

M

Q

Q

D

D

E

E

L

L

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L

L

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A

K

K

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F

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K

K

T

T

I

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H
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H

D

D

L

L

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N

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E

A

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I

I

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E

I

I

L

L

T

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N

P

P

A

A

N

N

A

D

Y

Y

V

V

E

K

binding adenosine-5'-triphosphate: F12 (= F13), K29 (= K30), T39 (= T40), S61 (= S62), G62 (= G63), S63 (= S64), G64 (= G65), K65 (= K66), S66 (= S67), T67 (= T68), Q111 (= Q112), K161 (≠ S162), Q162 (≠ E163), S164 (= S165), G166 (= G167), M167 (= M168), Q188 (≠ E189), H221 (= H222)

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
42% identity, 57% coverage: 33:264/408 of query aligns to 12:243/375 of 2d62A

query
sites
2d62A

I

I

L

W

K

K

A

R

T

F

G

G

C

D

T

V

V

T

A

A

V

V

K

K

D

D

A

L

N

S

L

L

S

E

I

I

N

K

E

D

G

G

E

E

I

F

F

L

V

V

I

L

M

L

G

G

L
x
P

S
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S

G
|
G

S
x
C

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

L

R

R

C

M

I

I

N

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R

G

L

L

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D

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A

A

M

M

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V

F

F

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F

Y

G

A

L

L

L

Y

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P

H

H

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M

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T

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V

L

Y

H

D

N

N

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E

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M

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A

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L

A

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L

Q

Q

S

R

K

Q

M

L

K

G

K

V

T

T

I

T

V

I

F

Y

I

V

T

T

H

H

D

D

L

Q

S

V

E

E

A

A

I

M

K

T

L

M

G

G

D

D

R

R

I

I

A

A

I

V

M

M

K

N

D

K

G

G

E

E

I

L

V

Q

Q

Q

I

V

G

G

T

T

S

P

E

D

E

E

I

V

L

Y

T

Y

E

K

P

P

A

V

N

N

A

T

Y

F

V

V

E

A

R

G

F

F

V

I

binding pyrophosphate 2-: P40 (≠ L61), S41 (= S62), G42 (= G63), C43 (≠ S64), G44 (= G65), K45 (= K66), T46 (≠ S67), T47 (= T68)

1g291 Malk (see paper)
39% identity, 57% coverage: 32:264/408 of query aligns to 8:240/372 of 1g291

query
sites
1g291

E

D

I

V

L

W

K

K

A

V

T

F

G

G

C

E

T

V

V

T

A

A

V

V

K

R

D

E

A

M

N

S

L

L

S

E

I

V

N

K

E

D

G

G

E

E

I

F

F

M

V

I

I

L

M

L

G

G

L
x
P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

L

R

R

C

M

I

I

N

A

R

G

L

L

I

E

R

E

P

P

T

S

S

R

G

G

E

Q

V

I

I

Y

I

I

N

G

G

D

T

K

D

L

I

V

A

A

K
x
D

V

P

S
x
E

D
x
K

K

G

E

I

L

F

L

V

Q

P

I

P

R
x
K

R
x
D

K

R

E

D

L

I

A

A

M

M

V

V

F

F

Q

Q

N

S

F

Y

G

A

L

L

L

Y

P

P

H

H

R

M

S

T

V

V

L

Y

H

D

N

N

I

I

A

A

F

F

G

P

L

L

E

K

L

L

Q

R

G

K

V

V

K

P

K

R

G

Q

E

E

R

I

E

D

Q

Q

K

R

A

V

M

R

E

E

S

V

M

A

Q

E

L

L

V

L

G

G

L

L

K

T

G

E

Y

L

E

L

N

N

Q

R

M

K

V

P

S

R

E

E

L

L

S

S

G

G

M

Q

Q

R

Q

Q

R

R

V

V

G

A

L

L

A

G

R

R

A

A

L

I

A

V

N

R

N

K

P

P

E

Q

V

V

L

F

L

L

M

M

D

D

E

E

A

P

F

L

S

S

A

N

L

L

D

D

P

A

L

K

I

L

R

R

V

V

Q

R

M

M

Q

R

D

A

E

E

L

L

L

K

T

K

L

L

Q

Q

S

R

K

Q

M

L

K

G

K

V

T

T

I

T

V

I

F

Y

I

V

T

T

H

H

D

D

L

Q

S

V

E

E

A

A

I

M

K

T

L

M

G

G

D

D

R

R

I

I

A

A

I

V

M

M

K

N

D

R

G

G

E

V

I

L

V

Q

Q

Q

I

V

G

G

T

S

S

P

E

D

E

E

I

V

L
x
Y

T
x
D

E

K

P

P

A

A

N

N

A

T

Y

F

V

V

E

A

R

G

F

F

V

I

binding magnesium ion: D69 (≠ K93), E71 (≠ S95), K72 (≠ D96), K79 (≠ R103), D80 (≠ R104), Y228 (≠ L252), D229 (≠ T253)
binding pyrophosphate 2-: P37 (≠ L61), S38 (= S62), G39 (= G63), C40 (≠ S64), G41 (= G65), K42 (= K66), T43 (≠ S67), T44 (= T68)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 341, 342, 359

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
43% identity, 53% coverage: 47:264/408 of query aligns to 37:248/378 of P69874

query
sites
P69874

N

D

L

L

S

T

I

I

N

N

E

N

G

G

E

E

I
x
F

F

L

V

T

I

L

M

L

G

G

L

P

S

S

G

G

S
x
C

G

G

K

K

S

T

T

T

L

V

L
|
L

R

R

C

L

I

I

N

A

R

G

L

L

I

E

R

T

P

V

T

D

S

S

G

G

E

R

V

I

I

M

I
x
L

N

D

G

N

T

E

D

D

I

I

A

T

K

H

V

V

S

P

D

A

K

E

E

-

L

-

Q

-

I

-

R

-

R

N

K

R

E

Y

L

V

A

N

M

T

V

V

F

F

Q

Q

N

S

F

Y

G

A

L

L

L

F

P

P

H

H

R

M

S

T

V

V

L

F

H

E

N

N

I

V

A

A

F

F

G

G

L

L

E

R

L

M

Q

Q

G

K

V

T

K

P

K

A

G

A

E

E

R

I

E

T

Q

P

K

R

A

V

M

M

E

E

S

A

M

L

Q

R

L

M

V
|
V

G

Q

L

L

K

E

G

T

Y

F

E

A

N

Q

Q

R

M

K

V

P

S

H

E

Q

L

L

S

S

G

G

M

Q

Q

Q

R

R

V

V

G

A

L

I

A

A

R

R

A

A

L

V

A

V

N

N

N

K

P

P

E

R

V

L

L

L

M

L

D
|
D

E

E

A

S

F

L

S

S

A

A

L

L

D

D

P

Y

L

K

I

L

R

R

V

K

Q

Q

M

M

Q

Q

D

N

E

E

L

L

L

K

T

A

L

L

Q

Q

S

R

K

K

M

L

K

G

K

I

T

T

I

F

V

V

F

F

I

V

T

T

H

H

D

D

L

Q

S

E

E

E

A

A

I

L

K

T

L

M

G

S

D

D

R

R

I

I

A

V

I

V

M

M

K

R

D

D

G

G

E

R

I

I

V

E

Q

Q

I

D

G

G

T

T

S

P

E

R

E

E

I

I

L

Y

T

E

E

E

P

P

A

K

N

N

A

L

Y

F

V

V

E

A

R

G

F

F

V

I

F45 (≠ I55) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ S64) mutation to T: Loss of ATPase activity and transport.
L60 (= L70) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ I86) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V151) mutation to M: Loss of ATPase activity and transport.
D172 (= D188) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

26 C→A: Lower ATPase activity and transport efficiency.
27 F→L: Lower ATPase activity and transport efficiency.
276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
38% identity, 55% coverage: 42:267/408 of query aligns to 20:244/343 of P30750

query
sites
P30750

A

A

V

L

K

N

D

N

A

V

N

S

L

L

S

H

I

V

N

P

E

A

G

G

E

Q

I

I

F

Y

V

G

I

V

M

I

G

G

L

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

C

C

I

V

N

N

R

L

L

L

I

E

R

R

P

P

T

T

S

E

G

G

E

S

V

V

I

L

I

V

N

D

G

G

T

Q

D

E

I

L

A

T

K

T

V

L

S

S

D

E

K

S

E

E

L

L

L

T

Q

K

I

A

R

R

K

-

E

Q

L

I

A

G

M

M

V

I

F

F

Q

Q

N

H

F

F

G

N

L

L

P

S

H

S

R

R

S

T

V

V

L

F

H

G

N

N

I

V

A

A

F

L

G

P

L

L

E

E

L

L

Q

D

G

N

V

T

K

P

K

K

G

D

E

E

R

V

E

K

Q

R

K

R

A

V

M

T

E

E

S

L

M

L

Q

S

L

L

V

V

G

G

L

L

K

G

G

D

Y

K

E

H

N

D

Q

S

M

Y

V

P

S

S

E

N

L

L

S

S

G

G

M

Q

Q

K

Q

Q

R

R

V

V

G

A

L

I

A

A

R

R

A

A

L

L

A

A

N

S

N

N

P

P

E

K

V

V

L

L

M

C

D

D

E
|
E

A

A

F

T

S

S

A

A

L

L

D

D

P

P

L

A

I

T

R

T

V

R

Q

S

M

I

Q

L

D

E

E

L

L

L

L

K

T

D

L

I

Q

N

S

R

K

R

M

L

K

G

K

L

T

T

I

I

V

L

F

L

I

I

T

T

H

H

D

E

L

M

S

D

E

V

A

V

I

K

K

R

L

I

G

C

D

D

R

C

I

V

A

A

I

V

M

I

K

S

D

N

G

G

E

E

I

L

V

I

Q

E

I

Q

G

D

T

T

S

V

E

S

E

E

I

V

L

F

T

S

E

H

P

P

A

K

N

T

A

P

Y

L

V

A

E

Q

R

K

F

F

V

I

E

Q

N

S

V

T

40:46 (vs. 62:68, 86% identical) binding
E166 (= E189) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
38% identity, 55% coverage: 42:267/408 of query aligns to 21:245/344 of 3tuzC

query
sites
3tuzC

A
|
A

V

L

K

N

D

N

A

V

N

S

L

L

S

H

I

V

N

P

E

A

G

G

E

Q

I

I

F

Y

V

G

I

V

M

I

G

G

L

A

S

S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

C

C

I

V

N

N

R

L

L

L

I

E

R

R

P

P

T

T

S

E

G

G

E

S

V

V

I

L

I

V

N

D

G

G

T

Q

D

E

I

L

A

T

K

T

V

L

S

S

D

E

K

S

E

E

L

L

L

T

Q

K

I

A

R

R

K

-

E

Q

L

I

A

G

M

M

V

I

F

F

Q

Q

N

H

F

F

G

N

L

L

P

S

H

S

R

R

S

T

V

V

L

F

H

G

N

N

I

V

A

A

F

L

G

P

L

L

E

E

L

L

Q

D

G

N

V

T

K

P

K

K

G

D

E

E

R

V

E

K

Q

R

K

R

A

V

M

T

E

E

S

L

M

L

Q

S

L

L

V

V

G

G

L

L

K

G

G

D

Y

K

E

H

N

D

Q

S

M

Y

V

P

S

S

E

N

L

L

S

S

G

G

M

Q

Q

K

Q

Q

R

R

V

V

G

A

L

I

A

A

R

R

A

A

L

L

A

A

N

S

N

N

P

P

E

K

V

V

L

L

M

C

D

D

E

Q

A

A

F

T

S

S

A

A

L

L

D

D

P

P

L

A

I

T

R

T

V

R

Q

S

M

I

Q

L

D

E

E

L

L

L

L

K

T

D

L

I

Q

N

S

R

K

R

M

L

K

G

K

L

T

T

I

I

V

L

F

L

I

I

T

T

H

H

D

E

L

M

S

D

E

V

A

V

I

K

K

R

L

I

G

C

D

D

R

C

I

V

A

A

I

V

M

I

K

S

D

N

G

G

E

E

I

L

V

I

Q

E

I

Q

G

D

T

T

S

V

E

S

E

E

I

V

L

F

T

S

E

H

P

P

A

K

N

T

A

P

Y

L

V

A

E

Q

R

K

F

F

V

I

E

Q

N

S

V

T

binding adenosine-5'-diphosphate: A21 (= A42), G42 (= G63), A43 (≠ S64), G44 (= G65), K45 (= K66), S46 (= S67), T47 (= T68)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 14
binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 312, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
38% identity, 55% coverage: 42:267/408 of query aligns to 21:245/344 of 3tuiC

query
sites
3tuiC

A

A

V

L

K

N

D

N

A

V

N

S

L

L

S

H

I

V

N

P

E

A

G

G

E

Q

I

I

F

Y

V

G

I

V

M

I

G

G

L

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

C

C

I

V

N

N

R

L

L

L

I

E

R

R

P

P

T

T

S

E

G

G

E

S

V

V

I

L

I

V

N

D

G

G

T

Q

D

E

I

L

A

T

K

T

V

L

S

S

D

E

K

S

E

E

L

L

L

T

Q

K

I

A

R

R

K

-

E

Q

L

I

A

G

M

M

V

I

F

F

Q

Q

N

H

F

F

G

N

L

L

P

S

H

S

R

R

S

T

V

V

L

F

H

G

N

N

I

V

A

A

F

L

G

P

L

L

E

E

L

L

Q

D

G

N

V

T

K

P

K

K

G

D

E

E

R

V

E

K

Q

R

K

R

A

V

M

T

E

E

S

L

M

L

Q

S

L

L

V

V

G

G

L

L

K

G

G

D

Y

K

E

H

N

D

Q

S

M

Y

V

P

S

S

E

N

L

L

S

S

G

G

M

Q

Q

K

Q

Q

R

R

V

V

G

A

L

I

A

A

R

R

A

A

L

L

A

A

N

S

N

N

P

P

E

K

V

V

L

L

M

C

D

D

E

Q

A

A

F

T

S

S

A

A

L

L

D

D

P

P

L

A

I

T

R

T

V

R

Q

S

M

I

Q

L

D

E

E

L

L

L

L

K

T

D

L

I

Q

N

S

R

K

R

M

L

K

G

K

L

T

T

I

I

V

L

F

L

I

I

T

T

H

H

D

E

L

M

S

D

E

V

A

V

I

K

K

R

L

I

G

C

D

D

R

C

I

V

A

A

I

V

M

I

K

S

D

N

G

G

E

E

I

L

V

I

Q

E

I

Q

G

D

T

T

S

V

E

S

E

E

I

V

L

F

T

S

E

H

P

P

A

K

N

T

A

P

Y

L

V

A

E

Q

R

K

F

F

V

I

E

Q

N

S

V

T

binding adenosine-5'-diphosphate: S41 (= S62), G42 (= G63), A43 (≠ S64), G44 (= G65), K45 (= K66), S46 (= S67), T47 (= T68)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 14

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
38% identity, 55% coverage: 42:267/408 of query aligns to 21:245/344 of 6cvlD

query
sites
6cvlD

A

A

V

L

K

N

D

N

A

V

N

S

L

L

S

H

I

V

N

P

E

A

G

G

E

Q

I

I

F

Y

V

G

I

V

M

I

G

G

L

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

C

C

I

V

N

N

R

L

L

L

I

E

R

R

P

P

T

T

S

E

G

G

E

S

V

V

I

L

I

V

N

D

G

G

T

Q

D

E

I

L

A

T

K

T

V

L

S

S

D

E

K

S

E

E

L

L

L

T

Q

K

I

A

R

R

K

-

E

Q

L

I

A

G

M

M

V

I

F

F

Q

Q

N

H

F

F

G

N

L

L

P

S

H

S

R

R

S

T

V

V

L

F

H

G

N

N

I

V

A

A

F

L

G

P

L

L

E

E

L

L

Q

D

G

N

V

T

K

P

K

K

G

D

E

E

R

V

E

K

Q

R

K

R

A

V

M

T

E

E

S

L

M

L

Q

S

L

L

V

V

G

G

L

L

K

G

G

D

Y

K

E

H

N

D

Q

S

M

Y

V

P

S

S

E
x
N

L
|
L

S
|
S

G
|
G

M
x
Q

Q

K

Q

Q

R

R

V

V

G

A

L

I

A

A

R

R

A

A

L

L

A

A

N

S

N

N

P

P

E

K

V

V

L

L

M

C

D

D

E

Q

A

A

F

T

S

S

A

A

L

L

D

D

P

P

L

A

I

T

R

T

V

R

Q

S

M

I

Q

L

D

E

E

L

L

L

L

K

T

D

L

I

Q

N

S

R

K

R

M

L

K

G

K

L

T

T

I

I

V

L

F

L

I

I

T

T

H
|
H

D

E

L

M

S

D

E

V

A

V

I

K

K

R

L

I

G

C

D

D

R

C

I

V

A

A

I

V

M

I

K

S

D

N

G

G

E

E

I

L

V

I

Q

E

I

Q

G

D

T

T

S

V

E

S

E

E

I

V

L

F

T

S

E

H

P

P

A

K

N

T

A

P

Y

L

V

A

E

Q

R

K

F

F

V

I

E

Q

N

S

V

T

binding phosphothiophosphoric acid-adenylate ester: S41 (= S62), G42 (= G63), A43 (≠ S64), G44 (= G65), K45 (= K66), S46 (= S67), T47 (= T68), N141 (≠ E163), L142 (= L164), S143 (= S165), G144 (= G166), G145 (= G167), Q146 (≠ M168), H200 (= H222)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12, 14, 19

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
41% identity, 55% coverage: 42:264/408 of query aligns to 19:235/393 of P9WQI3

query
sites
P9WQI3

A

A

V

V

K

R

D

D

A

L

N

N

L

L

S

T

I

I

N

A

E

D

G

G

E

E

I

F

F

L

V

I

I

L

M

V

G

G

L

P

S

S

G

G

S

C

G

G

K

K

S

T

T

T

L

T

L

L

R

N

C

M

I

I

N

A

R

G

L

L

I

E

R

D

P

I

T

S

S

S

G

G

E

E

V

L

I

R

I

I

N

A

G

G

T

E

D

R

I

V

A

N

K

E

V

K

S

A

D

P

K

K

E

D

L

-

Q

-

I

-

R

-

K

R

E

D

L

I

A

A

M

M

V

V

F

F

Q

Q

N

S

F

Y

G

A

L

L

L

Y

P

P

H

H

R

M

S

T

V

V

L

R

H

Q

N

N

I

I

A

A

F

F

G

P

L

L

E

T

L

L

Q

A

G

K

V

M

K

R

K

K

G

A

E

D

R

I

E

A

Q

Q

K

K

A

V

M

S

E

E

S

T

M

A

Q

K

L

I

V

L

G

D

L

L

K

T

G

N

Y

L

E

L

N

D

Q

R

M

K

V

P

S

S

E

Q

L

L

S

S

G

G

M

Q

Q

R

Q

Q

R

R

V

V

G

A

L

M

A

G

R

R

A

A

L

I

A

V

N

R

N

H

P

P

E

K

V

A

L

F

L

L

M

M

D

D

E

E

A

P

F

L

S

S

A

N

L

L

D

D

P

A

L

K

I

L

R

R

V

V

Q

Q

M

M

Q

R

D

G

E

E

L

I

L

A

T

Q

L

L

Q

Q

S

R

K

R

M

L

K

G

K

T

T

T

I

T

V

V

F

Y

I

V

T

T

H
|
H

D

D

L

Q

S

T

E

E

A

A

I

M

K

T

L

L

G

G

D

D

R

R

I

V

A

V

I

V

M

M

K

Y

D

G

G

G

E

I

I

A

V

Q

Q

Q

I

I

G

G

T

T

S

P

E

E

I

L

L

Y

T

E

E

R

P

P

A

A

N

N

A

L

Y

F

V

V

E

A

R

G

F

F

V

I

H193 (= H222) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

7cagC Mycobacterium smegmatis lpqy-sugabc complex in the catalytic intermediate state (see paper)
41% identity, 55% coverage: 41:264/408 of query aligns to 14:231/362 of 7cagC

query
sites
7cagC

V
x
A

A

A

V

V

K

K

D

E

A

F

N

S

L

M

S

T

I

I

N

A

E

D

G

G

E

E

I

F

F

I

V

I

I

L

M

V

G

G

L
x
P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

T

L

L

R

N

C

M

I

I

N

A

R

G

L

L

I

E

R

E

P

I

T

T

S

S

G

G

E

E

V

L

I

R

I

I

N

G

G

G

T

E

D

R

I

V

A

N

K

E

V

K

S

A

D

P

K

K

E

D

L

-

Q

-

I

-

R

-

K

R

E

D

L

I

A

A

M

M

V

V

F

F

Q
|
Q

N

S

F

Y

G

A

L

L

L

Y

P

P

H

H

R

M

S

T

V

V

L

R

H

Q

N

N

I

I

A

A

F

F

G

P

L

L

E

T

L

L

Q

A

G

K

V

V

K

P

K

K

G

A

E

E

R

I

E

A

Q

A

K

K

A

V

M

E

E

E

S

T

M

A

Q

K

L

I

V

L

G

D

L

L

K

S

G

E

Y
x
L

E

L

N

D

Q
x
R

M

K

V

P

S

G

E
x
Q

L

L

S
|
S

G
|
G

M
x
Q

Q

R

Q

Q

R

R

V

V

G

A

L

M

A

G

R

R

A

A

L

I

A

V

N

R

N

S

P

P

E

K

V

A

L

F

L

L

M

M

D

D

E

Q

A

P

F

L

S

S

A

N

L

L

D

D

P

A

L

K

I

L

R

R

V

V

Q

Q

M

M

Q

R

D

A

E

E

L

I

L

S

T

R

L

L

Q

Q

S

D

K

R

M

L

K

G

K

T

T

T

I

T

V

V

F

Y

I

V

T

T

H

H

D

D

L

Q

S

T

E

E

A

A

I

M

K

T

L

L

G

G

D

D

R

R

I

V

A

V

I

V

M

M

K

L

D

A

G

G

E

E

I

V

V

Q

Q

Q

I

I

G

G

T

T

S

P

E

D

E

E

I

L

L

Y

T

S

E

S

P

P

A

A

N

N

A

L

Y

F

V

V

E

A

R

G

F

F

V

I

binding adenosine-5'-triphosphate: A14 (≠ V41), P34 (≠ L61), S35 (= S62), G36 (= G63), C37 (≠ S64), G38 (= G65), K39 (= K66), S40 (= S67), T41 (= T68), Q79 (= Q112), L123 (≠ Y156), R126 (≠ Q159), Q130 (≠ E163), S132 (= S165), G133 (= G166), G134 (= G167), Q135 (≠ M168)
binding magnesium ion: S40 (= S67), Q79 (= Q112)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
40% identity, 58% coverage: 31:267/408 of query aligns to 5:239/240 of 4ymuJ

query
sites
4ymuJ

S

N

E

D

I

V

L

Y

K

K

A

N

T
x
F

G

G

C

S

T
x
L

V

E

A
x
V

V

L

K

K

D

G

A

V

N

T

L

L

S

K

I

V

N

N

E

K

G

G

E

E

I

V

F

V

V

V

I

I

M

I

G

G

L

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

C

C

I

I

N

N

R

L

L

L

I
x
E

R

E

P

P

T

T

S

K

G

G

E

E

V

V

I

F

I

I

N

D

G

G

T

V

D

K

I

I

-

N

A

G

K

K

V

V

S

N

D

I

K

N

E

K

L

V

L

-

Q

-

I

-

R

-

R

R

K

Q

E

K

L

V

A

G

M

M

V

V

F

F

Q

Q

N

H

F

F

G

N

L

L

L

F

P

P

H

H

R

L

S

T

V

A

L

I

H

E

N

N

I

I

A

T

F

L

G

A

-

P

L

V

E

K

L

V

Q

K

G

K

V

M

K

N

K

K

G

K

E

E

R

A

E

E

Q

E

K

L

A

A

M

V

E

D

S

L

M

L

Q

A

L

K

V

V

G

G

L

L

K

L

G

D

Y

K

E

K

N

D

Q

Q

M

Y

V

P

S

I

E

K

L

L

S

S

G

G

M

Q

Q

K

Q

Q

R

R

V

L

G

A

L

I

A

A

R

R

A

A

L

L

A

A

N

M

N

Q

P

P

E

E

V

V

L

M

L

L

M

F

D

D

E
|
E

A

P

F

T

S

S

A

A

L

L

D

D

P

P

L

-

I

-

R

-

V

-

Q

E

M

M

Q

V

D

K

E

E

L

V

L

L

T

N

L

V

Q

M

S

K

K

Q

M

L

K

A

K

N

-

E

-

G

-

M

T

T

I

M

V

V

F

V

I

V

T

T

H
|
H

D

E

L

M

S

G

E

F

A

A

I

R

K

E

L

V

G

G

D

D

R

R

I

V

A

I

I

F

M

M

K

D

D

D

G

G

E

V

I

I

V

V

Q

E

I

E

G

G

T

T

S

P

E

E

I

I

L

F

T

Y

E

R

P

A

A

K

N

N

A

E

Y

R

V

T

E

R

R

E

F

F

V

L

E

S

N

K

V

I

binding adenosine-5'-triphosphate: F11 (≠ T37), L14 (≠ T40), V16 (≠ A42), S36 (= S62), G37 (= G63), S38 (= S64), G39 (= G65), K40 (= K66), S41 (= S67), T42 (= T68), E51 (≠ I77), E162 (= E189), H194 (= H222)
binding magnesium ion: S41 (= S67), E162 (= E189)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
38% identity, 57% coverage: 33:264/408 of query aligns to 9:234/369 of P19566

query
sites
P19566

I

V

L

T

K

K

A

A

T

W

G

G

C

D

T

V

V

V

A

V

V

S

K

K

D

D

A

I

N

N

L

L

S

D

I

I

N

H

E

D

G

G

E

E

I

F

F

V

V

V

I

F

M

V

G

G

L

P

S

S

G

G

S

C

G

G

K

K

S

S

T

T

L

L

R

R

C

M

I

I

N

A

R

G

L

L

I

E

R

T

P

I

T

T

S

S

G

G

E

D

V

L

I

F

I

I

N

G

G

E

T

T

D

R

I

M

A

N

K

D

V

I

S

P

D

P

K

A

E

E

L

-

Q

-

I

-

R

-

K

R

E

G

L

V

A

G

M

M

V

V

F

F

Q

Q

N

S

F

Y

G

A

L
|
L

L

Y

P

P

H

H

R

L

S

S

V

V

L

A

H

E

N

N

I

M

A

S

F

F

G

G

L

L

E

K

L

L

Q

A

G

G

V

A

K

K

G

E

E

V

R

M

E

N

Q

Q

K

R

A

V

M

N

E

Q

S

V

M

A

Q

E

L

V

V

L

G

Q

L

L

K

A

G

H

Y

L

E

L

N

E

Q

R

M

K

V

P

S

K

E

A

L

L

S

S

G

G

M

Q

Q

R

Q

Q

R

R

V

V

G

A

L

I

A

G

R

R

A

T

L

L

A

V

N

A

N

E

P

P

E

R

V

V

L

F

L

L

M

L

D

D

E

E

A
x
P

F

L

S

S

A

N

L

L

D
|
D

P

A

L

A

I

L

R

R

V

V

Q

Q

M

M

Q

R

D

I

E

E

L

I

L

S

T

R

L

L

Q

H

S

K

K

R

M

L

K

G

K

R

T

T

I

M

V

I

F

Y

I

V

T

T

H

H

D

D

L

Q

S

V

E

E

A

A

I

M

K

T

L

L

G

A

D

D

R

K

I

I

A

V

I

V

M

L

K

D

D

A

G

G

E

R

I

V

V

A

Q

Q

I

V

G

G

T

K

S

P

E

L

E

E

I

L

L

Y

T

H

E

Y

P

P

A

A

N

D

A

R

Y

F

V

V

E

A

R

G

F

F

V

I

L86 (= L116) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (≠ A190) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D195) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
38% identity, 57% coverage: 33:264/408 of query aligns to 6:231/367 of 1q12A

query
sites
1q12A

I

V

L

T

K

K

A

A

T
x
W

G

G

C

E

T

V

V

V

A
x
V

V

S

K

K

D

D

A

I

N

N

L

L

S

D

I

I

N

H

E

E

G

G

E

E

I

F

F

V

V

V

I

F

M

V

G

G

L

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

C

M

I

I

N

A

R

G

L

L

I

E

R

T

P

I

T

T

S

S

G

G

E

D

V

L

I

F

I

I

N

G

G

E

T

K

D

R

I

M

A

N

K

D

V

T

S

P

D

P

K

A

E

E

L

-

Q

-

I

-

R

-

K

R

E

G

L

V

A

G

M

M

V

V

F

F

Q

Q

N

S

F

Y

G

A

L

L

L

Y

P

P

H

H

R

L

S

S

V

V

L

A

H

E

N

N

I

M

A

S

F

F

G

G

L

L

E

K

L

L

Q

A

G

G

V

A

K

K

G

E

E

V

R

I

E

N

Q

Q

K

R

A

V

M

N

E

Q

S

V

M

A

Q

E

L

V

V

L

G

Q

L

L

K

A

G

H

Y

L

E

L

N

D

Q
x
R

M

K

V

P

S
x
K

E
x
A

L
|
L

S
|
S

G
|
G

M
x
Q

Q

R

Q

Q

R

R

V

V

G

A

L

I

A

G

R

R

A

T

L

L

A

V

N

A

N

E

P

P

E

S

V

V

L

F

L

L

M

L

D

D

E

E

A

P

F

L

S

S

A
x
N

L

L

D

D

P

A

L

A

I

L

R

R

V

V

Q

Q

M

M

Q

R

D

I

E

E

L

I

L

S

T

R

L

L

Q

H

S

K

K

R

M

L

K

G

K

R

T

T

I

M

V

I

F

Y

I

V

T

T

H
|
H

D

D

L

Q

S

V

E

E

A

A

I

M

K

T

L

L

G

A

D

D

R

K

I

I

A

V

I

V

M

L

K

D

D

A

G

G

E

R

I

V

V

A

Q

Q

I

V

G

G

T

K

S

P

E

L

E

E

I

L

L

Y

T

H

E

Y

P

P

A

A

N

D

A

R

Y

F

V

V

E

A

R

G

F

F

V

I

binding adenosine-5'-triphosphate: W10 (≠ T37), V15 (≠ A42), S35 (= S62), G36 (= G63), C37 (≠ S64), G38 (= G65), K39 (= K66), S40 (= S67), T41 (= T68), R126 (≠ Q159), K129 (≠ S162), A130 (≠ E163), L131 (= L164), S132 (= S165), G133 (= G166), G134 (= G167), Q135 (≠ M168), N160 (≠ A193), H189 (= H222)

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
38% identity, 57% coverage: 33:264/408 of query aligns to 8:233/374 of 2awnB

query
sites
2awnB

I

V

L

T

K

K

A

A

T
x
W

G

G

C

E

T

V

V

V

A
x
V

V

S

K

K

D

D

A

I

N

N

L

L

S

D

I

I

N

H

E

E

G

G

E

E

I

F

F

V

V

V

I

F

M

V

G

G

L

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

C

M

I

I

N

A

R

G

L

L

I

E

R

T

P

I

T

T

S

S

G

G

E

D

V

L

I

F

I

I

N

G

G

E

T

K

D

R

I

M

A

N

K

D

V

T

S

P

D

P

K

A

E

E

L

-

Q

-

I

-

R

-

K

R

E

G

L

V

A

G

M

M

V

V

F

F

Q

Q

N

S

F

Y

G

A

L

L

L

Y

P

P

H

H

R

L

S

S

V

V

L

A

H

E

N

N

I

M

A

S

F

F

G

G

L

L

E

K

L

L

Q

A

G

G

V

A

K

K

G

E

E

V

R

I

E

N

Q

Q

K

R

A

V

M

N

E

Q

S

V

M

A

Q

E

L

V

V

L

G

Q

L

L

K

A

G

H

Y

L

E

L

N

D

Q

R

M

K

V

P

S

K

E

A

L

L

S

S

G

G

M

Q

Q

R

Q

Q

R

R

V

V

G

A

L

I

A

G

R

R

A

T

L

L

A

V

N

A

N

E

P

P

E

S

V

V

L

F

L

L

M

L

D

D

E

E

A

P

F

L

S

S

A

N

L

L

D

D

P

A

L

A

I

L

R

R

V

V

Q

Q

M

M

Q

R

D

I

E

E

L

I

L

S

T

R

L

L

Q

H

S

K

K

R

M

L

K

G

K

R

T

T

I

M

V

I

F

Y

I

V

T

T

H

H

D

D

L

Q

S

V

E

E

A

A

I

M

K

T

L

L

G

A

D

D

R

K

I

I

A

V

I

V

M

L

K

D

D

A

G

G

E

R

I

V

V

A

Q

Q

I

V

G

G

T

K

S

P

E

L

E

E

I

L

L

Y

T

H

E

Y

P

P

A

A

N

D

A

R

Y

F

V

V

E

A

R

G

F

F

V

I

binding adenosine-5'-diphosphate: W12 (≠ T37), V17 (≠ A42), S37 (= S62), G38 (= G63), C39 (≠ S64), G40 (= G65), K41 (= K66), S42 (= S67), T43 (= T68)

2awoA Crystal structure of the adp-mg-bound e. Coli malk (crystallized with adp-mg) (see paper)
38% identity, 57% coverage: 33:264/408 of query aligns to 8:233/372 of 2awoA

query
sites
2awoA

I

V

L

T

K

K

A

A

T
x
W

G

G

C

E

T

V

V

V

A
x
V

V

S

K

K

D

D

A

I

N

N

L

L

S

D

I

I

N

H

E

E

G

G

E

E

I

F

F

V

V

V

I

F

M

V

G

G

L

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

C

M

I

I

N

A

R

G

L

L

I

E

R

T

P

I

T

T

S

S

G

G

E

D

V

L

I

F

I

I

N

G

G

E

T

K

D

R

I

M

A

N

K

D

V

T

S

P

D

P

K

A

E

E

L

-

Q

-

I

-

R

-

K

R

E

G

L

V

A

G

M

M

V

V

F

F

Q

Q

N

S

F

Y

G

A

L

L

L

Y

P

P

H

H

R

L

S

S

V

V

L

A

H

E

N

N

I

M

A

S

F

F

G

G

L

L

E

K

L

L

Q

A

G

G

V

A

K

K

G

E

E

V

R

I

E

N

Q

Q

K

R

A

V

M

N

E

Q

S

V

M

A

Q

E

L

V

V

L

G

Q

L

L

K

A

G

H

Y

L

E

L

N

D

Q

R

M

K

V

P

S

K

E

A

L

L

S

S

G

G

M

Q

Q

R

Q

Q

R

R

V

V

G

A

L

I

A

G

R

R

A

T

L

L

A

V

N

A

N

E

P

P

E

S

V

V

L

F

L

L

M

L

D
|
D

E

E

A

P

F

L

S

S

A

N

L

L

D

D

P

A

L

A

I

L

R

R

V

V

Q

Q

M

M

Q

R

D

I

E

E

L

I

L

S

T

R

L

L

Q

H

S

K

K

R

M

L

K

G

K

R

T

T

I

M

V

I

F

Y

I

V

T

T

H

H

D

D

L

Q

S

V

E

E

A

A

I

M

K

T

L

L

G

A

D

D

R

K

I

I

A

V

I

V

M

L

K

D

D

A

G

G

E

R

I

V

V

A

Q

Q

I

V

G

G

T

K

S

P

E

L

E

E

I

L

L

Y

T

H

E

Y

P

P

A

A

N

D

A

R

Y

F

V

V

E

A

R

G

F

F

V

I

binding adenosine-5'-diphosphate: W12 (≠ T37), V17 (≠ A42), S37 (= S62), G38 (= G63), C39 (≠ S64), G40 (= G65), K41 (= K66), S42 (= S67), T43 (= T68)
binding magnesium ion: S42 (= S67), D157 (= D188)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
38% identity, 57% coverage: 33:264/408 of query aligns to 9:234/371 of P68187

query
sites
P68187

I

V

L

T

K

K

A

A

T

W

G

G

C

E

T

V

V

V

A

V

V

S

K

K

D

D

A

I

N

N

L

L

S

D

I

I

N

H

E

E

G

G

E

E

I

F

F

V

V

V

I

F

M

V

G

G

L

P

S

S

G

G

S

C

G

G

K

K

S

S

T

T

L

L

R

R

C

M

I

I

N

A

R

G

L

L

I

E

R

T

P

I

T

T

S

S

G

G

E

D

V

L

I

F

I

I

N

G

G

E

T

K

D

R

I

M

A

N

K

D

V

T

S

P

D

P

K

A

E

E

L

-

Q

-

I

-

R

-

K

R

E

G

L

V

A

G

M

M

V

V

F

F

Q

Q

N

S

F

Y

G
x
A

L

L

L

Y

P

P

H

H

R

L

S

S

V

V

L

A

H

E

N

N

I

M

A

S

F

F

G

G

L

L

E

K

L

L

Q

A

G

G

V

A

K
|
K

K

K

G

E